AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280008866.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008866.01.T01 MTR_2g449850 56.863 102 12 1 1 70 362 463 2.93e-29 108
MsG0280008866.01.T01 MTR_3g434850 52.427 103 17 1 1 71 354 456 1.29e-27 103
MsG0280008866.01.T01 MTR_3g498755 70.175 57 17 0 15 71 391 447 2.73e-25 97.1
MsG0280008866.01.T01 MTR_1g040370 52.475 101 16 1 1 69 315 415 5.96e-25 95.9
MsG0280008866.01.T01 MTR_8g099930 68.421 57 18 0 13 69 406 462 6.23e-24 93.6
MsG0280008866.01.T01 MTR_7g021130 67.273 55 18 0 15 69 403 457 3.26e-23 91.7
MsG0280008866.01.T01 MTR_1g102130 44.554 101 24 1 1 69 390 490 2.44e-22 89.4
MsG0280008866.01.T01 MTR_3g090150 53.571 56 26 0 14 69 408 463 1.51e-18 78.6
MsG0280008866.01.T01 MTR_3g090130 50.000 60 30 0 14 73 401 460 1.20e-17 75.9
MsG0280008866.01.T01 MTR_5g072570 51.724 58 28 0 15 72 407 464 3.30e-17 74.7
MsG0280008866.01.T01 MTR_2g039800 66.667 45 15 0 24 68 50 94 7.10e-16 66.6
MsG0280008866.01.T01 MTR_2g062990 46.667 60 31 1 12 71 406 464 1.97e-14 66.6
MsG0280008866.01.T01 MTR_2g062980 47.368 57 29 1 15 71 409 464 2.37e-14 66.6
MsG0280008866.01.T01 MTR_2g063000 45.000 60 32 1 12 71 376 434 1.39e-13 64.3
MsG0280008866.01.T01 MTR_2g063000 45.000 60 32 1 12 71 407 465 1.40e-13 64.3
MsG0280008866.01.T01 MTR_2g081620 45.000 60 32 1 12 71 407 465 2.67e-13 63.5
MsG0280008866.01.T01 MTR_7g013790 55.556 54 23 1 18 71 414 466 1.77e-12 61.2
MsG0280008866.01.T01 MTR_2g012860 48.148 54 27 1 18 71 423 475 4.35e-12 60.1
MsG0280008866.01.T01 MTR_3g079620 43.860 57 31 1 14 70 444 499 3.07e-11 57.8
MsG0280008866.01.T01 MTR_1g095560 37.705 61 37 1 12 72 400 459 3.17e-11 57.8
MsG0280008866.01.T01 MTR_1g095540 35.593 59 37 1 12 70 400 457 6.47e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008866.01.T01 AT2G33530 66.129 62 20 1 9 69 401 462 5.67e-22 88.2
MsG0280008866.01.T01 AT1G28110 62.903 62 22 1 9 69 397 458 1.35e-21 87.0
MsG0280008866.01.T01 AT1G28110 62.903 62 22 1 9 69 397 458 1.35e-21 87.0
MsG0280008866.01.T01 AT5G42240 56.364 55 24 0 15 69 410 464 1.62e-17 75.5
MsG0280008866.01.T01 AT1G43780 52.459 61 29 0 12 72 413 473 1.83e-16 72.8
MsG0280008866.01.T01 AT5G42230 50.847 59 29 0 15 73 406 464 2.07e-16 72.4
MsG0280008866.01.T01 AT5G42230 50.847 59 29 0 15 73 406 464 2.07e-16 72.4
MsG0280008866.01.T01 AT2G12480 41.791 67 39 0 13 79 401 467 3.06e-16 72.0
MsG0280008866.01.T01 AT2G12480 41.791 67 39 0 13 79 377 443 3.69e-16 71.6
MsG0280008866.01.T01 AT2G12480 41.791 67 39 0 13 79 375 441 3.76e-16 71.6
MsG0280008866.01.T01 AT4G30610 55.769 52 22 1 18 69 412 462 1.19e-14 67.4
MsG0280008866.01.T01 AT2G12480 41.176 68 39 1 13 79 375 442 1.61e-14 67.0
MsG0280008866.01.T01 AT2G24010 59.615 52 20 1 18 69 412 462 2.58e-14 66.6
MsG0280008866.01.T01 AT2G24010 59.615 52 20 1 18 69 416 466 2.63e-14 66.6
MsG0280008866.01.T01 AT2G24010 59.615 52 20 1 18 69 401 451 3.21e-14 66.2
MsG0280008866.01.T01 AT4G30810 44.286 70 37 2 14 83 408 475 7.27e-13 62.4
MsG0280008866.01.T01 AT5G08260 44.615 65 35 1 7 70 413 477 7.94e-13 62.4
MsG0280008866.01.T01 AT2G05850 41.818 55 32 0 15 69 430 484 2.80e-12 60.8
MsG0280008866.01.T01 AT3G63470 45.902 61 33 0 14 74 442 502 3.51e-12 60.5
MsG0280008866.01.T01 AT2G24000 52.941 51 23 1 18 68 348 397 4.68e-12 60.1
MsG0280008866.01.T01 AT2G24000 52.941 51 23 1 18 68 411 460 7.05e-12 59.7
MsG0280008866.01.T01 AT3G52010 41.379 58 34 0 12 69 427 484 8.35e-12 59.3
MsG0280008866.01.T01 AT1G11080 48.214 56 28 1 14 69 435 489 1.39e-11 58.9
MsG0280008866.01.T01 AT1G11080 48.214 56 28 1 14 69 435 489 1.46e-11 58.5
MsG0280008866.01.T01 AT3G52000 41.379 58 34 0 12 69 422 479 2.39e-11 58.2
MsG0280008866.01.T01 AT3G17180 40.580 69 36 1 2 70 413 476 5.33e-11 57.0
MsG0280008866.01.T01 AT1G61130 46.429 56 29 1 14 69 406 460 5.42e-11 57.0
MsG0280008866.01.T01 AT3G02110 50.877 57 27 1 13 69 416 471 7.79e-11 56.6

Find 31 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCGCGCAGGCTGAGAAAA+TGG 0.282066 2:+40191138 None:intergenic
CACTTGTTAAATCAGGGGTT+CGG 0.299311 2:-40191576 MsG0280008866.01.T01:CDS
TTCTCAGCCTGCGCGAGCTT+TGG 0.334267 2:-40191133 MsG0280008866.01.T01:CDS
TTTATCATTTGCAACAATAA+GGG 0.347974 2:-40191178 MsG0280008866.01.T01:CDS
TTAGGGCCACTTGTTAAATC+AGG 0.357193 2:-40191583 MsG0280008866.01.T01:CDS
AACCCTACAATCTCTATGTT+AGG 0.358096 2:-40191601 MsG0280008866.01.T01:exon
CCAACTATTAGTGAATTTGT+TGG 0.379336 2:-40191075 MsG0280008866.01.T01:CDS
GGGTTCGGGTTCTAGCTTAC+AGG 0.380620 2:-40191561 MsG0280008866.01.T01:intron
GCAGGATGGACGGAGGGTTA+TGG 0.411732 2:-40191207 MsG0280008866.01.T01:CDS
CCAACAAATTCACTAATAGT+TGG 0.411985 2:+40191075 None:intergenic
TATTTAATTGTTCAGGTGGC+AGG 0.438998 2:-40191225 MsG0280008866.01.T01:intron
GCGAGCTTTGGTACTATTCA+AGG 0.441740 2:-40191121 MsG0280008866.01.T01:CDS
ACTTGTTAAATCAGGGGTTC+GGG 0.495035 2:-40191575 MsG0280008866.01.T01:CDS
GGTACTATTCAAGGCATTTG+TGG 0.519304 2:-40191112 MsG0280008866.01.T01:CDS
ACCCTACAATCTCTATGTTA+GGG 0.521246 2:-40191600 MsG0280008866.01.T01:exon
GGCCCTAACATAGAGATTGT+AGG 0.521971 2:+40191598 None:intergenic
GCCCTAACATAGAGATTGTA+GGG 0.527268 2:+40191599 None:intergenic
CTATTCAAGGCATTTGTGGA+AGG 0.529889 2:-40191108 MsG0280008866.01.T01:CDS
CTCGTATAGAGTTTGGTATG+AGG 0.542677 2:-40191346 MsG0280008866.01.T01:intron
TAGGGCCACTTGTTAAATCA+GGG 0.543896 2:-40191582 MsG0280008866.01.T01:CDS
CGAACCCCTGATTTAACAAG+TGG 0.550763 2:+40191577 None:intergenic
AGTTTGGTATGAGGGTAGCC+AGG 0.551338 2:-40191337 MsG0280008866.01.T01:intron
TTATCATTTGCAACAATAAG+GGG 0.561572 2:-40191177 MsG0280008866.01.T01:CDS
TGTTCAGGTGGCAGGATGGA+CGG 0.564756 2:-40191217 MsG0280008866.01.T01:intron
TCGTATAGAGTTTGGTATGA+GGG 0.567116 2:-40191345 MsG0280008866.01.T01:intron
TATTCAAGGCATTTGTGGAA+GGG 0.571085 2:-40191107 MsG0280008866.01.T01:CDS
TCAGGTGGCAGGATGGACGG+AGG 0.589632 2:-40191214 MsG0280008866.01.T01:intron
CAGGTGGCAGGATGGACGGA+GGG 0.591184 2:-40191213 MsG0280008866.01.T01:CDS
AATAGTACCAAAGCTCGCGC+AGG 0.591962 2:+40191126 None:intergenic
AGGGCCACTTGTTAAATCAG+GGG 0.612046 2:-40191581 MsG0280008866.01.T01:CDS
TAATTGTTCAGGTGGCAGGA+TGG 0.638374 2:-40191221 MsG0280008866.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTGCTAAAGAATAAAA+TGG - Chr2:40191189-40191208 MsG0280008866.01.T01:CDS 15.0%
!!! ATTTTGATATTTAATTGTTC+AGG - Chr2:40191824-40191843 MsG0280008866.01.T01:intron 15.0%
!! ATGTATATAAGTAACTAAGT+TGG + Chr2:40191540-40191559 None:intergenic 20.0%
!! TATCCTATTAAATATTTACC+TGG + Chr2:40191740-40191759 None:intergenic 20.0%
!! TTATTTACACTTGTAATTAG+TGG + Chr2:40191255-40191274 None:intergenic 20.0%
!! TTTATCATTTGCAACAATAA+GGG - Chr2:40191878-40191897 MsG0280008866.01.T01:intron 20.0%
!!! TTTTATCATTTGCAACAATA+AGG - Chr2:40191877-40191896 MsG0280008866.01.T01:intron 20.0%
! CTTATAGAAAAATCGAGAAA+TGG - Chr2:40191078-40191097 MsG0280008866.01.T01:CDS 25.0%
! GAGAAAAAAACAAAAAGCTT+TGG + Chr2:40191597-40191616 None:intergenic 25.0%
! TAGCCAGGTAAATATTTAAT+AGG - Chr2:40191734-40191753 MsG0280008866.01.T01:intron 25.0%
! TTATCATTTGCAACAATAAG+GGG - Chr2:40191879-40191898 MsG0280008866.01.T01:intron 25.0%
! TTGATATTTAATTGTTCAGG+TGG - Chr2:40191827-40191846 MsG0280008866.01.T01:intron 25.0%
!! ATATGACATTGATTGATAGT+AGG + Chr2:40191569-40191588 None:intergenic 25.0%
!! TATATAAGTAACTAAGTTGG+AGG + Chr2:40191537-40191556 None:intergenic 25.0%
AATGGTTAAGGAAAGAGAAA+TGG - Chr2:40191096-40191115 MsG0280008866.01.T01:CDS 30.0%
ACTGTAACTAATTGTTGAAG+TGG - Chr2:40191289-40191308 MsG0280008866.01.T01:intron 30.0%
CCAACAAATTCACTAATAGT+TGG + Chr2:40191984-40192003 None:intergenic 30.0%
CCAACTATTAGTGAATTTGT+TGG - Chr2:40191981-40192000 MsG0280008866.01.T01:five_prime_UTR 30.0%
GAAAAATCGAGAAATGGTTA+AGG - Chr2:40191084-40191103 MsG0280008866.01.T01:CDS 30.0%
! CTCTAAAAGACGGAAAATAT+TGG + Chr2:40191406-40191425 None:intergenic 30.0%
! GGTATACGATTTTCAAAATC+TGG - Chr2:40191430-40191449 MsG0280008866.01.T01:intron 30.0%
! TATACCAGAACTCTAAAAGA+CGG + Chr2:40191416-40191435 None:intergenic 30.0%
AACCCTACAATCTCTATGTT+AGG - Chr2:40191455-40191474 MsG0280008866.01.T01:intron 35.0%
ACCCTACAATCTCTATGTTA+GGG - Chr2:40191456-40191475 MsG0280008866.01.T01:intron 35.0%
TATTTAATTGTTCAGGTGGC+AGG - Chr2:40191831-40191850 MsG0280008866.01.T01:intron 35.0%
TCGTATAGAGTTTGGTATGA+GGG - Chr2:40191711-40191730 MsG0280008866.01.T01:intron 35.0%
TCTAAACCTGTACATCAACA+TGG + Chr2:40191148-40191167 None:intergenic 35.0%
! TATTCAAGGCATTTGTGGAA+GGG - Chr2:40191949-40191968 MsG0280008866.01.T01:intron 35.0%
!!! AAATACCCTTTTTTGCGACT+CGG - Chr2:40191641-40191660 MsG0280008866.01.T01:intron 35.0%
!!! TTTTCCGTCTTTTAGAGTTC+TGG - Chr2:40191409-40191428 MsG0280008866.01.T01:intron 35.0%
CAACAGACTCGTATAGAGTT+TGG - Chr2:40191703-40191722 MsG0280008866.01.T01:intron 40.0%
CTCGTATAGAGTTTGGTATG+AGG - Chr2:40191710-40191729 MsG0280008866.01.T01:intron 40.0%
CTGATACCATGTTGATGTAC+AGG - Chr2:40191139-40191158 MsG0280008866.01.T01:CDS 40.0%
GCCCTAACATAGAGATTGTA+GGG + Chr2:40191460-40191479 None:intergenic 40.0%
TCGGTCGTTAATAAAGGGAT+TGG - Chr2:40191660-40191679 MsG0280008866.01.T01:intron 40.0%
! ACTTGTTAAATCAGGGGTTC+GGG - Chr2:40191481-40191500 MsG0280008866.01.T01:intron 40.0%
! CACTTGTTAAATCAGGGGTT+CGG - Chr2:40191480-40191499 MsG0280008866.01.T01:intron 40.0%
! CTATTCAAGGCATTTGTGGA+AGG - Chr2:40191948-40191967 MsG0280008866.01.T01:intron 40.0%
! GGTACTATTCAAGGCATTTG+TGG - Chr2:40191944-40191963 MsG0280008866.01.T01:intron 40.0%
! GTTAATAAAGGGATTGGCGA+AGG - Chr2:40191666-40191685 MsG0280008866.01.T01:intron 40.0%
! TAGGGCCACTTGTTAAATCA+GGG - Chr2:40191474-40191493 MsG0280008866.01.T01:intron 40.0%
! TTAGGGCCACTTGTTAAATC+AGG - Chr2:40191473-40191492 MsG0280008866.01.T01:intron 40.0%
!!! TTGTTTTTTTCTCCCTGCAG+TGG - Chr2:40191604-40191623 MsG0280008866.01.T01:exon 40.0%
AACGACCGAGTCGCAAAAAA+GGG + Chr2:40191649-40191668 None:intergenic 45.0%
CGAACCCCTGATTTAACAAG+TGG + Chr2:40191482-40191501 None:intergenic 45.0%
GGCCCTAACATAGAGATTGT+AGG + Chr2:40191461-40191480 None:intergenic 45.0%
TAACGACCGAGTCGCAAAAA+AGG + Chr2:40191650-40191669 None:intergenic 45.0%
TAATTGTTCAGGTGGCAGGA+TGG - Chr2:40191835-40191854 MsG0280008866.01.T01:intron 45.0%
! AGGGCCACTTGTTAAATCAG+GGG - Chr2:40191475-40191494 MsG0280008866.01.T01:intron 45.0%
! GCGAGCTTTGGTACTATTCA+AGG - Chr2:40191935-40191954 MsG0280008866.01.T01:intron 45.0%
! TATCAGAGCTCATCTAGCTC+TGG + Chr2:40191125-40191144 None:intergenic 45.0%
!! GCGACTCGGTCGTTAATAAA+GGG - Chr2:40191655-40191674 MsG0280008866.01.T01:intron 45.0%
!! TGCGACTCGGTCGTTAATAA+AGG - Chr2:40191654-40191673 MsG0280008866.01.T01:intron 45.0%
AATAGTACCAAAGCTCGCGC+AGG + Chr2:40191933-40191952 None:intergenic 50.0%
AGTTTGGTATGAGGGTAGCC+AGG - Chr2:40191719-40191738 MsG0280008866.01.T01:intron 50.0%
GAGTCTGTTGCAGTCAATCC+AGG + Chr2:40191693-40191712 None:intergenic 50.0%
AGAGTCCTGATCTCCACTGC+AGG + Chr2:40191620-40191639 None:intergenic 55.0%
GAGTCCTGATCTCCACTGCA+GGG + Chr2:40191619-40191638 None:intergenic 55.0%
TGTTCAGGTGGCAGGATGGA+CGG - Chr2:40191839-40191858 MsG0280008866.01.T01:intron 55.0%
TTCTCCCTGCAGTGGAGATC+AGG - Chr2:40191612-40191631 MsG0280008866.01.T01:five_prime_UTR 55.0%
! AAAGGGATTGGCGAAGGACC+TGG - Chr2:40191672-40191691 MsG0280008866.01.T01:intron 55.0%
!! GGGTTCGGGTTCTAGCTTAC+AGG - Chr2:40191495-40191514 MsG0280008866.01.T01:intron 55.0%
GCAGGATGGACGGAGGGTTA+TGG - Chr2:40191849-40191868 MsG0280008866.01.T01:intron 60.0%
GCTCGCGCAGGCTGAGAAAA+TGG + Chr2:40191921-40191940 None:intergenic 60.0%
TTCTCAGCCTGCGCGAGCTT+TGG - Chr2:40191923-40191942 MsG0280008866.01.T01:intron 60.0%
CAGGTGGCAGGATGGACGGA+GGG - Chr2:40191843-40191862 MsG0280008866.01.T01:intron 65.0%
TCAGGTGGCAGGATGGACGG+AGG - Chr2:40191842-40191861 MsG0280008866.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 40191050 40192028 40191050 ID=MsG0280008866.01;Name=MsG0280008866.01
Chr2 mRNA 40191050 40192028 40191050 ID=MsG0280008866.01.T01;Parent=MsG0280008866.01;Name=MsG0280008866.01.T01;_AED=0.50;_eAED=0.66;_QI=96|0|0|1|0.33|0.5|4|0|83
Chr2 exon 40191979 40192028 40191979 ID=MsG0280008866.01.T01:exon:1714;Parent=MsG0280008866.01.T01
Chr2 exon 40191562 40191654 40191562 ID=MsG0280008866.01.T01:exon:1713;Parent=MsG0280008866.01.T01
Chr2 exon 40191338 40191359 40191338 ID=MsG0280008866.01.T01:exon:1712;Parent=MsG0280008866.01.T01
Chr2 exon 40191050 40191232 40191050 ID=MsG0280008866.01.T01:exon:1711;Parent=MsG0280008866.01.T01
Chr2 five_prime_UTR 40191979 40192028 40191979 ID=MsG0280008866.01.T01:five_prime_utr;Parent=MsG0280008866.01.T01
Chr2 five_prime_UTR 40191609 40191654 40191609 ID=MsG0280008866.01.T01:five_prime_utr;Parent=MsG0280008866.01.T01
Chr2 CDS 40191562 40191608 40191562 ID=MsG0280008866.01.T01:cds;Parent=MsG0280008866.01.T01
Chr2 CDS 40191338 40191359 40191338 ID=MsG0280008866.01.T01:cds;Parent=MsG0280008866.01.T01
Chr2 CDS 40191050 40191232 40191050 ID=MsG0280008866.01.T01:cds;Parent=MsG0280008866.01.T01
Gene Sequence

>MsG0280008866.01.T01

ATGTTAGGGCCACTTGTTAAATCAGGGGTTCGGGTTCTAGCTTACAGAGTTTGGTATGAGGGTAGCCAGGTGGCAGGATGGACGGAGGGTTATGGTGAGTTTTTATCATTTGCAACAATAAGGGGAGCAGCTCATGCTGCTCCATTTTCTCAGCCTGCGCGAGCTTTGGTACTATTCAAGGCATTTGTGGAAGGGAAGTCTCTTCCAACTATTAGTGAATTTGTTGGTAAACTTGATAGAACTGAACTGTAA

Protein sequence

>MsG0280008866.01.T01

MLGPLVKSGVRVLAYRVWYEGSQVAGWTEGYGEFLSFATIRGAAHAAPFSQPARALVLFKAFVEGKSLPTISEFVGKLDRTEL*