Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0014330 | Msa0111510 | 0.800419 | 1.454206e-48 | -8.615850e-47 |
| Msa0014330 | Msa0227480 | 0.820440 | 6.853200e-53 | -8.615850e-47 |
| Msa0014330 | Msa0232850 | 0.819723 | 9.996579e-53 | -8.615850e-47 |
| Msa0014330 | Msa0267000 | 0.802816 | 4.687822e-49 | -8.615850e-47 |
| Msa0014330 | Msa0267010 | 0.809152 | 2.179015e-50 | -8.615850e-47 |
| Msa0014330 | Msa0342240 | 0.814853 | 1.246709e-51 | -8.615850e-47 |
| Msa0014330 | Msa0360510 | 0.802635 | 5.108729e-49 | -8.615850e-47 |
| Msa0014330 | Msa0384800 | 0.842489 | 2.507139e-58 | -8.615850e-47 |
| Msa0014330 | Msa0414420 | 0.813180 | 2.915735e-51 | -8.615850e-47 |
| Msa0014330 | Msa0435230 | 0.815382 | 9.513156e-52 | -8.615850e-47 |
| Msa0014330 | Msa0452900 | 0.827166 | 1.817267e-54 | -8.615850e-47 |
| Msa0014330 | Msa0479340 | 0.805878 | 1.079179e-49 | -8.615850e-47 |
| Msa0014330 | Msa0598780 | 0.806965 | 6.366987e-50 | -8.615850e-47 |
| Msa0014330 | Msa0639610 | 0.801373 | 9.286448e-49 | -8.615850e-47 |
| Msa0014330 | Msa0726140 | 0.833631 | 4.772415e-56 | -8.615850e-47 |
| Msa0014330 | Msa0735080 | 0.801523 | 8.650407e-49 | -8.615850e-47 |
| Msa0014330 | Msa0743810 | 0.831051 | 2.078205e-55 | -8.615850e-47 |
| Msa0014330 | Msa0745060 | 0.819501 | 1.123414e-52 | -8.615850e-47 |
| Msa0014330 | Msa0783160 | 0.819241 | 1.287927e-52 | -8.615850e-47 |
| Msa0014330 | Msa0825860 | 0.826328 | 2.880727e-54 | -8.615850e-47 |
| Msa0014330 | Msa0846350 | 0.809845 | 1.546971e-50 | -8.615850e-47 |
| Msa0014330 | Msa0865320 | 0.806796 | 6.913453e-50 | -8.615850e-47 |
| Msa0014330 | Msa0907260 | 0.841360 | 4.982488e-58 | -8.615850e-47 |
| Msa0014330 | Msa0956270 | 0.837066 | 6.472728e-57 | -8.615850e-47 |
| Msa0014330 | Msa0957860 | 0.807395 | 5.162740e-50 | -8.615850e-47 |
| Msa0014330 | Msa0957960 | 0.808154 | 3.560512e-50 | -8.615850e-47 |
| Msa0014330 | Msa0989060 | 0.827425 | 1.575495e-54 | -8.615850e-47 |
| Msa0014330 | Msa0998690 | 0.807336 | 5.311839e-50 | -8.615850e-47 |
| Msa0014330 | Msa1016280 | 0.831571 | 1.547718e-55 | -8.615850e-47 |
| Msa0014330 | Msa1025360 | 0.812523 | 4.061829e-51 | -8.615850e-47 |
| Msa0014330 | Msa1025370 | 0.804099 | 2.541111e-49 | -8.615850e-47 |
| Msa0014330 | Msa1044830 | 0.804623 | 1.976331e-49 | -8.615850e-47 |
| Msa0014330 | Msa1044840 | 0.816348 | 5.793787e-52 | -8.615850e-47 |
| Msa0014330 | Msa1092990 | 0.815746 | 7.894936e-52 | -8.615850e-47 |
| Msa0014330 | Msa1100980 | 0.822856 | 1.893770e-53 | -8.615850e-47 |
| Msa0014330 | Msa1109440 | 0.804969 | 1.673248e-49 | -8.615850e-47 |
| Msa0014330 | Msa1135740 | 0.800546 | 1.370201e-48 | -8.615850e-47 |
| Msa0014330 | Msa1136090 | 0.808391 | 3.170128e-50 | -8.615850e-47 |
| Msa0014330 | Msa1182090 | 0.810503 | 1.116174e-50 | -8.615850e-47 |
| Msa0014330 | Msa1187950 | 0.804377 | 2.223841e-49 | -8.615850e-47 |
| Msa0014330 | Msa1192640 | 0.806790 | 6.931832e-50 | -8.615850e-47 |
| Msa0014330 | Msa1269670 | 0.824490 | 7.846012e-54 | -8.615850e-47 |
| Msa0014330 | Msa1270230 | 0.808413 | 3.135747e-50 | -8.615850e-47 |
| Msa0014330 | Msa1270240 | 0.817791 | 2.745143e-52 | -8.615850e-47 |
| Msa0014330 | Msa1273230 | 0.810581 | 1.073549e-50 | -8.615850e-47 |
| Msa0014330 | Msa1283330 | 0.828271 | 9.862901e-55 | -8.615850e-47 |
| Msa0014330 | Msa1285930 | 0.826428 | 2.727489e-54 | -8.615850e-47 |
| Msa0014330 | Msa1287800 | 0.807788 | 4.260427e-50 | -8.615850e-47 |
| Msa0014330 | Msa1287810 | 0.824126 | 9.558341e-54 | -8.615850e-47 |
| Msa0014330 | Msa1289430 | 0.803397 | 3.554997e-49 | -8.615850e-47 |
| Msa0014330 | Msa1369430 | 0.805815 | 1.112524e-49 | -8.615850e-47 |
| Msa0014330 | Msa1386330 | 0.800995 | 1.109444e-48 | -8.615850e-47 |
| Msa0014330 | Msa1404000 | 0.841570 | 4.384990e-58 | -8.615850e-47 |
| Msa0014330 | Msa1422180 | 0.812837 | 3.467651e-51 | -8.615850e-47 |
| Msa0014330 | Msa1433120 | 0.805363 | 1.384349e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGCACATGTTTGAGGATT+TGG | 0.219887 | 1_1:+22706271 | Msa0014330:CDS |
| GTGCACATGTTTGAGGATTT+GGG | 0.340939 | 1_1:+22706272 | Msa0014330:CDS |
| GTGTTGACGTTGGCGAGAAT+TGG | 0.358791 | 1_1:+22706205 | Msa0014330:CDS |
| TGTTGACGTTGGCGAGAATT+GGG | 0.392391 | 1_1:+22706206 | Msa0014330:CDS |
| ACCAACAGTGCACATGTTTG+AGG | 0.441066 | 1_1:+22706265 | Msa0014330:CDS |
| CAGTGGCGCGTGAATATGAC+GGG | 0.497291 | 1_1:+22706151 | Msa0014330:CDS |
| TCCTCAAACATGTGCACTGT+TGG | 0.514518 | 1_1:-22706266 | Msa0014320:intron |
| GCAGAATTACTTCTCTTCCA+TGG | 0.524122 | 1_1:-22706233 | Msa0014320:intron |
| GGCGAGAATTGGGGTTGCCA+TGG | 0.537039 | 1_1:+22706216 | Msa0014330:CDS |
| CATATGAGAAGTACGAGGTA+AGG | 0.545942 | 1_1:-22706303 | Msa0014320:CDS |
| ACGTCTACAAAGAGTGATAG+TGG | 0.552529 | 1_1:+22706183 | Msa0014330:CDS |
| GCAGTGGCGCGTGAATATGA+CGG | 0.576805 | 1_1:+22706150 | Msa0014330:CDS |
| CGGAAGGAGACTTATGGCAG+TGG | 0.599116 | 1_1:+22706134 | None:intergenic |
| GTTGACGTTGGCGAGAATTG+GGG | 0.622074 | 1_1:+22706207 | Msa0014330:CDS |
| GCGCGTGAATATGACGGGGA+CGG | 0.631699 | 1_1:+22706156 | Msa0014330:CDS |
| AGTGATAGTGGTGTTGACGT+TGG | 0.695592 | 1_1:+22706195 | Msa0014330:CDS |
| ATCATCATATGAGAAGTACG+AGG | 0.768520 | 1_1:-22706308 | Msa0014320:CDS |
| AGTGGCGCGTGAATATGACG+GGG | 0.771284 | 1_1:+22706152 | Msa0014330:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACGTCTACAAAGAGTGATAG+TGG | + | chr1_1:22706183-22706202 | Msa0014330:CDS | 40.0% | |
| GCAGAATTACTTCTCTTCCA+TGG | - | chr1_1:22706236-22706255 | Msa0014320:intron | 40.0% | |
| AGTGCACATGTTTGAGGATT+TGG | + | chr1_1:22706271-22706290 | Msa0014330:CDS | 40.0% | |
| GTGCACATGTTTGAGGATTT+GGG | + | chr1_1:22706272-22706291 | Msa0014330:CDS | 40.0% | |
| ! | CATATGAGAAGTACGAGGTA+AGG | - | chr1_1:22706306-22706325 | Msa0014320:CDS | 40.0% |
| !! | AGTGATAGTGGTGTTGACGT+TGG | + | chr1_1:22706195-22706214 | Msa0014330:CDS | 45.0% |
| TGTTGACGTTGGCGAGAATT+GGG | + | chr1_1:22706206-22706225 | Msa0014330:CDS | 45.0% | |
| ACCAACAGTGCACATGTTTG+AGG | + | chr1_1:22706265-22706284 | Msa0014330:CDS | 45.0% | |
| TCCTCAAACATGTGCACTGT+TGG | - | chr1_1:22706269-22706288 | Msa0014320:CDS | 45.0% | |
| ! | GTGTTGACGTTGGCGAGAAT+TGG | + | chr1_1:22706205-22706224 | Msa0014330:CDS | 50.0% |
| GTTGACGTTGGCGAGAATTG+GGG | + | chr1_1:22706207-22706226 | Msa0014330:CDS | 50.0% | |
| GCAGTGGCGCGTGAATATGA+CGG | + | chr1_1:22706150-22706169 | Msa0014330:CDS | 55.0% | |
| CAGTGGCGCGTGAATATGAC+GGG | + | chr1_1:22706151-22706170 | Msa0014330:CDS | 55.0% | |
| AGTGGCGCGTGAATATGACG+GGG | + | chr1_1:22706152-22706171 | Msa0014330:CDS | 55.0% | |
| ! | GCGCGTGAATATGACGGGGA+CGG | + | chr1_1:22706156-22706175 | Msa0014330:CDS | 60.0% |
| GGCGAGAATTGGGGTTGCCA+TGG | + | chr1_1:22706216-22706235 | Msa0014330:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 22706147 | 22706326 | 22706147 | ID=Msa0014330;Name=Msa0014330 |
| chr1_1 | mRNA | 22706147 | 22706326 | 22706147 | ID=Msa0014330-mRNA-1;Parent=Msa0014330;Name=Msa0014330-mRNA-1;_AED=0.46;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|59 |
| chr1_1 | exon | 22706147 | 22706326 | 22706147 | ID=Msa0014330-mRNA-1:exon:7976;Parent=Msa0014330-mRNA-1 |
| chr1_1 | CDS | 22706147 | 22706326 | 22706147 | ID=Msa0014330-mRNA-1:cds;Parent=Msa0014330-mRNA-1 |
| Gene Sequence |
| Protein sequence |
