AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1369430


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1369430 MtrunA17_Chr2g0320884 73.913 115 20 3 1 105 163 277 6.85e-21 85.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 13 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTAGGCCTCTTCTTTCTT+TGG 0.167682 tig0018782:+133488 None:intergenic
GAAGATTGTGATGATGAAAT+TGG 0.327410 tig0018782:-133951 Msa1369430:CDS
CCTTTAGAACATTATCTTCA+AGG 0.416307 tig0018782:-133308 Msa1369430:intron
AATGCCACTCCTGAGAAAAC+TGG 0.441679 tig0018782:-133427 Msa1369430:intron
CTTTAGAACATTATCTTCAA+GGG 0.524266 tig0018782:-133307 Msa1369430:intron
TGTGTCATTACCTTCTTCAC+AGG 0.526680 tig0018782:+133599 None:intergenic
GGAAGAGACTCCTGTGAAGA+AGG 0.538391 tig0018782:-133609 Msa1369430:intron
TGTGAAGGTTGTTGATCTAA+AGG 0.545160 tig0018782:-133985 Msa1369430:CDS
TGAGAGTGATGAAGATGACG+AGG 0.555243 tig0018782:-133630 Msa1369430:CDS
AGGTGGACCAAAGAAAGAAG+AGG 0.653905 tig0018782:-133495 Msa1369430:CDS
TGCACCTGCAAAACATGTGA+AGG 0.659451 tig0018782:-134000 Msa1369430:CDS
TTATCTTCAAGGGCATTGTG+AGG 0.660935 tig0018782:-133297 Msa1369430:CDS
AATTGGAAGCTCTGATGATG+AGG 0.661842 tig0018782:-133934 Msa1369430:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTACAATATATAATACAACT+TGG + tig0018782:133895-133914 None:intergenic 15.0%
!! TTACAATATATAATACAACT+TGG + tig0018782:133895-133914 None:intergenic 15.0%
!!! ATAAATTTGTTAAAAGTGCT+AGG - tig0018782:133479-133498 Msa1369430:CDS 20.0%
!!! TAAATTTGTTAAAAGTGCTA+GGG - tig0018782:133480-133499 Msa1369430:CDS 20.0%
!!! TTAGTATCTTTTTGTCTAAT+TGG - tig0018782:133925-133944 Msa1369430:intron 20.0%
!!! ATAAATTTGTTAAAAGTGCT+AGG - tig0018782:133479-133498 Msa1369430:CDS 20.0%
!!! TAAATTTGTTAAAAGTGCTA+GGG - tig0018782:133480-133499 Msa1369430:CDS 20.0%
!!! TTAGTATCTTTTTGTCTAAT+TGG - tig0018782:133925-133944 Msa1369430:intron 20.0%
!!! TTTTTAGCATCAGCTTTTTT+AGG + tig0018782:133268-133287 None:intergenic 25.0%
! CTTCGTATATATTAGATTGA+AGG + tig0018782:133449-133468 None:intergenic 25.0%
!!! AAATTTGTTAAAAGTGCTAG+GGG - tig0018782:133481-133500 Msa1369430:CDS 25.0%
! TATATTTACTGAAGATGAGA+AGG + tig0018782:133541-133560 None:intergenic 25.0%
!! TTGATCTTGATAATTGTAGA+TGG - tig0018782:133607-133626 Msa1369430:intron 25.0%
!!! CTTTAGAACATTATCTTCAA+GGG - tig0018782:133984-134003 Msa1369430:CDS 25.0%
!!! TTTTTAGCATCAGCTTTTTT+AGG + tig0018782:133268-133287 None:intergenic 25.0%
! CTTCGTATATATTAGATTGA+AGG + tig0018782:133449-133468 None:intergenic 25.0%
!!! AAATTTGTTAAAAGTGCTAG+GGG - tig0018782:133481-133500 Msa1369430:CDS 25.0%
! TATATTTACTGAAGATGAGA+AGG + tig0018782:133541-133560 None:intergenic 25.0%
!! TTGATCTTGATAATTGTAGA+TGG - tig0018782:133607-133626 Msa1369430:intron 25.0%
!!! CTTTAGAACATTATCTTCAA+GGG - tig0018782:133984-134003 Msa1369430:CDS 25.0%
! GAAGATTGTGATGATGAAAT+TGG - tig0018782:133340-133359 Msa1369430:intron 30.0%
! TGCTATAAGTTGTCCATTTT+TGG - tig0018782:133411-133430 Msa1369430:intron 30.0%
!! AATTTGTTAAAAGTGCTAGG+GGG - tig0018782:133482-133501 Msa1369430:CDS 30.0%
!! AACTTGTCATTCTGTTTTGA+AGG - tig0018782:133776-133795 Msa1369430:intron 30.0%
!!! GTTTTTGAAGCAGATTCATT+AGG + tig0018782:133822-133841 None:intergenic 30.0%
CCTTGAAGATAATGTTCTAA+AGG + tig0018782:133986-134005 None:intergenic 30.0%
!! CCTTTAGAACATTATCTTCA+AGG - tig0018782:133983-134002 Msa1369430:CDS 30.0%
AGTATGTATGCAACCAAAAA+TGG - tig0018782:134026-134045 Msa1369430:CDS 30.0%
GTATGTATGCAACCAAAAAT+GGG - tig0018782:134027-134046 Msa1369430:CDS 30.0%
!! ATGTCCATTTTTCCCATTTT+TGG + tig0018782:134042-134061 None:intergenic 30.0%
! GAAGATTGTGATGATGAAAT+TGG - tig0018782:133340-133359 Msa1369430:intron 30.0%
! TGCTATAAGTTGTCCATTTT+TGG - tig0018782:133411-133430 Msa1369430:intron 30.0%
!! AATTTGTTAAAAGTGCTAGG+GGG - tig0018782:133482-133501 Msa1369430:CDS 30.0%
!! AACTTGTCATTCTGTTTTGA+AGG - tig0018782:133776-133795 Msa1369430:intron 30.0%
!!! GTTTTTGAAGCAGATTCATT+AGG + tig0018782:133822-133841 None:intergenic 30.0%
CCTTGAAGATAATGTTCTAA+AGG + tig0018782:133986-134005 None:intergenic 30.0%
!! CCTTTAGAACATTATCTTCA+AGG - tig0018782:133983-134002 Msa1369430:CDS 30.0%
AGTATGTATGCAACCAAAAA+TGG - tig0018782:134026-134045 Msa1369430:CDS 30.0%
GTATGTATGCAACCAAAAAT+GGG - tig0018782:134027-134046 Msa1369430:CDS 30.0%
!! ATGTCCATTTTTCCCATTTT+TGG + tig0018782:134042-134061 None:intergenic 30.0%
TGTGAAGGTTGTTGATCTAA+AGG - tig0018782:133306-133325 Msa1369430:intron 35.0%
GACAGTATGACTACCAAAAA+TGG + tig0018782:133427-133446 None:intergenic 35.0%
TCATATAGTGTTCTCAGCTT+AGG - tig0018782:133508-133527 Msa1369430:intron 35.0%
!!! TTGTCATTCTGTTTTGAAGG+TGG - tig0018782:133779-133798 Msa1369430:intron 35.0%
GCAACCAAAAATGGGAAAAA+TGG - tig0018782:134035-134054 Msa1369430:CDS 35.0%
TGTGAAGGTTGTTGATCTAA+AGG - tig0018782:133306-133325 Msa1369430:intron 35.0%
GACAGTATGACTACCAAAAA+TGG + tig0018782:133427-133446 None:intergenic 35.0%
TCATATAGTGTTCTCAGCTT+AGG - tig0018782:133508-133527 Msa1369430:intron 35.0%
!!! TTGTCATTCTGTTTTGAAGG+TGG - tig0018782:133779-133798 Msa1369430:intron 35.0%
GCAACCAAAAATGGGAAAAA+TGG - tig0018782:134035-134054 Msa1369430:CDS 35.0%
! ACAACCTTCACATGTTTTGC+AGG + tig0018782:133298-133317 None:intergenic 40.0%
! AATTGGAAGCTCTGATGATG+AGG - tig0018782:133357-133376 Msa1369430:intron 40.0%
! TGTGTCATTACCTTCTTCAC+AGG + tig0018782:133695-133714 None:intergenic 40.0%
CATTAGGCCTCTTCTTTCTT+TGG + tig0018782:133806-133825 None:intergenic 40.0%
!! TTATCTTCAAGGGCATTGTG+AGG - tig0018782:133994-134013 Msa1369430:CDS 40.0%
! ACAACCTTCACATGTTTTGC+AGG + tig0018782:133298-133317 None:intergenic 40.0%
! AATTGGAAGCTCTGATGATG+AGG - tig0018782:133357-133376 Msa1369430:intron 40.0%
! TGTGTCATTACCTTCTTCAC+AGG + tig0018782:133695-133714 None:intergenic 40.0%
CATTAGGCCTCTTCTTTCTT+TGG + tig0018782:133806-133825 None:intergenic 40.0%
!! TTATCTTCAAGGGCATTGTG+AGG - tig0018782:133994-134013 Msa1369430:CDS 40.0%
TGCACCTGCAAAACATGTGA+AGG - tig0018782:133291-133310 Msa1369430:CDS 45.0%
TGAGAGTGATGAAGATGACG+AGG - tig0018782:133661-133680 Msa1369430:CDS 45.0%
AGGTGGACCAAAGAAAGAAG+AGG - tig0018782:133796-133815 Msa1369430:intron 45.0%
AATGCCACTCCTGAGAAAAC+TGG - tig0018782:133864-133883 Msa1369430:intron 45.0%
! TTGGTCTCACCAGTTTTCTC+AGG + tig0018782:133876-133895 None:intergenic 45.0%
TGCACCTGCAAAACATGTGA+AGG - tig0018782:133291-133310 Msa1369430:CDS 45.0%
TGAGAGTGATGAAGATGACG+AGG - tig0018782:133661-133680 Msa1369430:CDS 45.0%
AGGTGGACCAAAGAAAGAAG+AGG - tig0018782:133796-133815 Msa1369430:intron 45.0%
AATGCCACTCCTGAGAAAAC+TGG - tig0018782:133864-133883 Msa1369430:intron 45.0%
! TTGGTCTCACCAGTTTTCTC+AGG + tig0018782:133876-133895 None:intergenic 45.0%
GGAAGAGACTCCTGTGAAGA+AGG - tig0018782:133682-133701 Msa1369430:intron 50.0%
! CTCACCAGTTTTCTCAGGAG+TGG + tig0018782:133871-133890 None:intergenic 50.0%
GGAAGAGACTCCTGTGAAGA+AGG - tig0018782:133682-133701 Msa1369430:intron 50.0%
! CTCACCAGTTTTCTCAGGAG+TGG + tig0018782:133871-133890 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
tig0018782 gene 133250 134063 133250 ID=Msa1369430;Name=Msa1369430
tig0018782 mRNA 133250 134063 133250 ID=Msa1369430-mRNA-1;Parent=Msa1369430;Name=Msa1369430-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0|0|0|1|1|1|4|0|122
tig0018782 exon 133935 134063 133935 ID=Msa1369430-mRNA-1:exon:1129;Parent=Msa1369430-mRNA-1
tig0018782 exon 133610 133687 133610 ID=Msa1369430-mRNA-1:exon:1128;Parent=Msa1369430-mRNA-1
tig0018782 exon 133428 133515 133428 ID=Msa1369430-mRNA-1:exon:1127;Parent=Msa1369430-mRNA-1
tig0018782 exon 133250 133323 133250 ID=Msa1369430-mRNA-1:exon:1126;Parent=Msa1369430-mRNA-1
tig0018782 CDS 133935 134063 133935 ID=Msa1369430-mRNA-1:cds;Parent=Msa1369430-mRNA-1
tig0018782 CDS 133610 133687 133610 ID=Msa1369430-mRNA-1:cds;Parent=Msa1369430-mRNA-1
tig0018782 CDS 133428 133515 133428 ID=Msa1369430-mRNA-1:cds;Parent=Msa1369430-mRNA-1
tig0018782 CDS 133250 133323 133250 ID=Msa1369430-mRNA-1:cds;Parent=Msa1369430-mRNA-1
Gene Sequence

>Msa1369430

TTGAAGGTTTCTGAACCTAAAAAAGCTGATGCTAAAAATGCTGCACCTGCAAAACATGTGAAGGTTGTTGATCTAAAGGATGTGAAAGATGAAGATTGTGATGATGAAATTGGAAGCTCTGATGATGAGATGGAGAATTCTGATAGTGATAGTCAAGATGAAGATGAGAGTGATGAAGATGACGAGGAAGAGACTCCTGTGAAGAAGGTGGACCAAAGAAAGAAGAGGCCTAATGAATCTGCTTCAAAAACTCTAGTTTCTAAGAAAACAAAAAATGCCACTCCTGAGAAAACTGAACATTATCTTCAAGGGCATTGTGAGGTTTCGAAAAAGTATGTATGCAACCAAAAATGGGAAAAATGGACATAG

Protein sequence

>Msa1369430

LKVSEPKKADAKNAAPAKHVKVVDLKDVKDEDCDDEIGSSDDEMENSDSDSQDEDESDEDDEEETPVKKVDQRKKRPNESASKTLVSKKTKNATPEKTEHYLQGHCEVSKKYVCNQKWEKWT*