AlfalfaGEDB | Msa0049690

M. sativa cultivar zhongmu-4 / Msa0049690

KEGG: K02960
GO: GO:0000462 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005840 GO:0005844 GO:0005911 GO:0006139 GO:0006364 GO:0006396 GO:0006412 GO:0006518 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0015935 GO:0016020 GO:0016070 GO:0016072 GO:0019538 GO:0022613 GO:0022626 GO:0022627 GO:0030054 GO:0030312 GO:0030490 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034645 GO:0034660 GO:0042254 GO:0042274 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046483 GO:0055044 GO:0070013 GO:0071704 GO:0071840 GO:0071944 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00380.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0049690	XP_003631001.1	99.286	140	1	0	1	140	1	140	2.61e-96	283

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0049690	sp\|P46293\|RS16_GOSHI	93.571	140	9	0	1	140	6	145	1.09e-92	267

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0049690	G7LBJ0	99.286	140	1	0	1	140	1	140	1.25e-96	283

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0049690	Msa0221030	0.811794	5.857144e-51	-8.615850e-47
Msa0049690	Msa0257450	0.807487	4.935133e-50	-8.615850e-47
Msa0049690	Msa0269180	0.807360	5.251439e-50	-8.615850e-47
Msa0049690	Msa0297830	0.812516	4.076374e-51	-8.615850e-47
Msa0049690	Msa0310080	0.834361	3.133159e-56	-8.615850e-47
Msa0049690	Msa0335840	0.820929	5.289392e-53	-8.615850e-47
Msa0049690	Msa0443390	0.803556	3.294734e-49	-8.615850e-47
Msa0049690	Msa0584030	0.814079	1.849292e-51	-8.615850e-47
Msa0049690	Msa0590940	0.820065	8.350316e-53	-8.615850e-47
Msa0049690	Msa0604960	0.805229	1.476667e-49	-8.615850e-47
Msa0049690	Msa0625570	0.997535	2.688922e-244	-8.615850e-47
Msa0049690	Msa0631250	0.804182	2.442168e-49	-8.615850e-47
Msa0049690	Msa0712610	0.814419	1.555581e-51	-8.615850e-47
Msa0049690	Msa0729200	0.811996	5.294194e-51	-8.615850e-47
Msa0049690	Msa0769590	0.805636	1.213384e-49	-8.615850e-47
Msa0049690	Msa0792420	0.839476	1.549007e-57	-8.615850e-47
Msa0049690	Msa0888810	0.826584	2.503688e-54	-8.615850e-47
Msa0049690	Msa0901360	0.800060	1.720509e-48	-8.615850e-47
Msa0049690	Msa0915540	0.805638	1.211908e-49	-8.615850e-47
Msa0049690	Msa0945480	0.828502	8.674551e-55	-8.615850e-47
Msa0049690	Msa1037290	0.813501	2.479387e-51	-8.615850e-47
Msa0049690	Msa1039520	0.805632	1.215602e-49	-8.615850e-47
Msa0049690	Msa1078870	0.817753	2.800458e-52	-8.615850e-47
Msa0049690	Msa1080350	0.805012	1.639136e-49	-8.615850e-47
Msa0049690	Msa1256840	0.838768	2.363172e-57	-8.615850e-47
Msa0049690	Msa1311400	0.819622	1.054485e-52	-8.615850e-47
Msa0049690	Msa1321790	0.854039	1.604806e-61	-8.615850e-47
Msa0049690	Msa1332250	0.813379	2.637533e-51	-8.615850e-47
Msa0049690	Msa1338190	0.825312	5.020487e-54	-8.615850e-47
Msa0049690	Msa1349570	0.805472	1.313250e-49	-8.615850e-47
Msa0049690	Msa1356790	0.827909	1.205639e-54	-8.615850e-47
Msa0049690	Msa1362790	0.818482	1.915616e-52	-8.615850e-47
Msa0049690	Msa1368920	0.810692	1.015679e-50	-8.615850e-47
Msa0049690	Msa1369190	0.822168	2.735865e-53	-8.615850e-47
Msa0049690	Msa1374240	0.814457	1.525939e-51	-8.615850e-47
Msa0049690	Msa1426120	0.815114	1.091054e-51	-8.615850e-47
Msa0049690	Msa1426490	0.800425	1.449890e-48	-8.615850e-47
Msa0049690	Msa1437280	0.998282	9.655506e-261	-8.615850e-47
Msa0049690	Msa1456050	0.818078	2.365008e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0049690	MtrunA17_Chr8g0392251	99.286	140	1	1	140	1	140	2.40e-100	283
Msa0049690	MtrunA17_Chr8g0392191	99.286	140	1	1	140	1	140	2.40e-100	283
Msa0049690	MtrunA17_Chr5g0432551	98.571	140	2	1	140	1	140	6.11e-100	282
Msa0049690	MtrunA17_Chr4g0044571	94.286	140	8	1	140	4	143	7.79e-96	272

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0049690	AT2G09990.1	89.209	139	15	2	140	8	146	1.92e-91	261
Msa0049690	AT5G18380.1	88.489	139	16	2	140	8	146	3.25e-91	261
Msa0049690	AT3G04230.1	83.453	139	23	2	140	8	146	6.19e-85	245
Msa0049690	AT5G18380.2	86.400	125	17	2	126	8	132	1.67e-78	229
Msa0049690	AT5G18380.3	86.111	108	15	2	109	8	115	5.55e-67	199

Find 38 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCTAACGATAAGATTTC+TGG	0.210396	1_2:-3163377	None:intergenic
CCGCAACGGCGTTCTTCTTC+CGG	0.308388	1_2:-3162996	None:intergenic
GCCTGAGATTCTTCGTTTCA+AGG	0.344740	1_2:+3163083	Msa0049690:CDS
CGCTGTGAGCCTAAGAAGTT+TGG	0.359075	1_2:+3163330	Msa0049690:CDS
TCAGAGTATTGCTAAGGCTT+TGG	0.386737	1_2:+3163209	Msa0049690:CDS
CCACGACCACCAAACTTCTT+AGG	0.436009	1_2:-3163339	None:intergenic
TACGAACCAATCCTCCTTCT+CGG	0.444217	1_2:+3163108	Msa0049690:CDS
GTTTCGCCGGCGTTGATATG+AGG	0.478501	1_2:+3163139	Msa0049690:CDS
GCCTTGAAACGAAGAATCTC+AGG	0.478888	1_2:-3163084	None:intergenic
CTCGGAAGACACCGTTTCGC+CGG	0.484088	1_2:+3163126	Msa0049690:CDS
ATGGAACAAGTGCAATGCTT+TGG	0.487256	1_2:+3162973	Msa0049690:CDS
CAACGGCTGTCCAATCGAAC+TGG	0.488470	1_2:+3163056	Msa0049690:CDS
GTGGTCGTGGTGCTCGTGCT+AGG	0.502073	1_2:+3163352	Msa0049690:CDS
ACAGAGTAAGAAAGAGATCA+AGG	0.505686	1_2:+3163260	Msa0049690:CDS
CCTAAGAAGTTTGGTGGTCG+TGG	0.511400	1_2:+3163339	Msa0049690:CDS
AGGATCCGTGTGAAGGGAGG+AGG	0.521481	1_2:+3163159	Msa0049690:CDS
TCATATCAACGCCGGCGAAA+CGG	0.524265	1_2:-3163137	None:intergenic
TCAGGCTCAACCAGTTCGAT+TGG	0.524396	1_2:-3163066	None:intergenic
AACAAGTGCAATGCTTTGGC+CGG	0.546867	1_2:+3162977	Msa0049690:CDS
AACGGTGTCTTCCGAGAAGG+AGG	0.549270	1_2:-3163119	None:intergenic
TGTGAGCCTAAGAAGTTTGG+TGG	0.558448	1_2:+3163333	Msa0049690:CDS
GACCGCGACCTCTCTTGCAG+TGG	0.558828	1_2:-3163023	None:intergenic
ACCCACTGCAAGAGAGGTCG+CGG	0.562945	1_2:+3163021	Msa0049690:CDS
CATTCGTCAGAGTATTGCTA+AGG	0.576627	1_2:+3163203	Msa0049690:CDS
TGATATGAGGATCCGTGTGA+AGG	0.579448	1_2:+3163152	Msa0049690:CDS
GCGGTAACCCACTGCAAGAG+AGG	0.580471	1_2:+3163015	Msa0049690:CDS
TTCTTAGGCTCACAGCGTCT+AGG	0.590494	1_2:-3163324	None:intergenic
TGTCTTCCGAGAAGGAGGAT+TGG	0.592227	1_2:-3163114	None:intergenic
GATATGAGGATCCGTGTGAA+GGG	0.594162	1_2:+3163153	Msa0049690:CDS
CTTGCAGTGGGTTACCGCAA+CGG	0.599991	1_2:-3163010	None:intergenic
ACCGCGACCTCTCTTGCAGT+GGG	0.603590	1_2:-3163022	None:intergenic
CGCGGTCTGATCAAGATCAA+CGG	0.609302	1_2:+3163039	Msa0049690:CDS
CTTAGCAATACTCTGACGAA+TGG	0.614188	1_2:-3163202	None:intergenic
ATGAGGATCCGTGTGAAGGG+AGG	0.615322	1_2:+3163156	Msa0049690:CDS
CGAAACGGTGTCTTCCGAGA+AGG	0.622095	1_2:-3163122	None:intergenic
TGTGACCTCCTCCCTTCACA+CGG	0.629480	1_2:-3163164	None:intergenic
CCGGAAGAAGAACGCCGTTG+CGG	0.651449	1_2:+3162996	Msa0049690:CDS
ACGGATCCTCATATCAACGC+CGG	0.685813	1_2:-3163145	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACAGAGTAAGAAAGAGATCA+AGG	+	chr1_2:3163260-3163279	Msa0049690:CDS	35.0%
	ATGGAACAAGTGCAATGCTT+TGG	+	chr1_2:3162973-3162992	Msa0049690:CDS	40.0%
	CTTAGCAATACTCTGACGAA+TGG	-	chr1_2:3163205-3163224	None:intergenic	40.0%
!!	CATTCGTCAGAGTATTGCTA+AGG	+	chr1_2:3163203-3163222	Msa0049690:CDS	40.0%
!!	TCAGAGTATTGCTAAGGCTT+TGG	+	chr1_2:3163209-3163228	Msa0049690:CDS	40.0%
	AACAAGTGCAATGCTTTGGC+CGG	+	chr1_2:3162977-3162996	Msa0049690:CDS	45.0%
	GCCTGAGATTCTTCGTTTCA+AGG	+	chr1_2:3163083-3163102	Msa0049690:CDS	45.0%
	GCCTTGAAACGAAGAATCTC+AGG	-	chr1_2:3163087-3163106	None:intergenic	45.0%
	TACGAACCAATCCTCCTTCT+CGG	+	chr1_2:3163108-3163127	Msa0049690:CDS	45.0%
	TGATATGAGGATCCGTGTGA+AGG	+	chr1_2:3163152-3163171	Msa0049690:CDS	45.0%
	GATATGAGGATCCGTGTGAA+GGG	+	chr1_2:3163153-3163172	Msa0049690:CDS	45.0%
	TGTGAGCCTAAGAAGTTTGG+TGG	+	chr1_2:3163333-3163352	Msa0049690:CDS	45.0%
	CGCGGTCTGATCAAGATCAA+CGG	+	chr1_2:3163039-3163058	Msa0049690:CDS	50.0%
	TCAGGCTCAACCAGTTCGAT+TGG	-	chr1_2:3163069-3163088	None:intergenic	50.0%
	TGTCTTCCGAGAAGGAGGAT+TGG	-	chr1_2:3163117-3163136	None:intergenic	50.0%
	TCATATCAACGCCGGCGAAA+CGG	-	chr1_2:3163140-3163159	None:intergenic	50.0%
	ACGGATCCTCATATCAACGC+CGG	-	chr1_2:3163148-3163167	None:intergenic	50.0%
	TTCTTAGGCTCACAGCGTCT+AGG	-	chr1_2:3163327-3163346	None:intergenic	50.0%
	CGCTGTGAGCCTAAGAAGTT+TGG	+	chr1_2:3163330-3163349	Msa0049690:CDS	50.0%
	CCACGACCACCAAACTTCTT+AGG	-	chr1_2:3163342-3163361	None:intergenic	50.0%
!	CCTAAGAAGTTTGGTGGTCG+TGG	+	chr1_2:3163339-3163358	Msa0049690:CDS	50.0%
!!	CTTGCAGTGGGTTACCGCAA+CGG	-	chr1_2:3163013-3163032	None:intergenic	55.0%
	CAACGGCTGTCCAATCGAAC+TGG	+	chr1_2:3163056-3163075	Msa0049690:CDS	55.0%
	AACGGTGTCTTCCGAGAAGG+AGG	-	chr1_2:3163122-3163141	None:intergenic	55.0%
	CGAAACGGTGTCTTCCGAGA+AGG	-	chr1_2:3163125-3163144	None:intergenic	55.0%
!!	GTTTCGCCGGCGTTGATATG+AGG	+	chr1_2:3163139-3163158	Msa0049690:CDS	55.0%
	ATGAGGATCCGTGTGAAGGG+AGG	+	chr1_2:3163156-3163175	Msa0049690:CDS	55.0%
	TGTGACCTCCTCCCTTCACA+CGG	-	chr1_2:3163167-3163186	None:intergenic	55.0%
	CCGCAACGGCGTTCTTCTTC+CGG	-	chr1_2:3162999-3163018	None:intergenic	60.0%
	CCGGAAGAAGAACGCCGTTG+CGG	+	chr1_2:3162996-3163015	Msa0049690:CDS	60.0%
	GCGGTAACCCACTGCAAGAG+AGG	+	chr1_2:3163015-3163034	Msa0049690:CDS	60.0%
	ACCCACTGCAAGAGAGGTCG+CGG	+	chr1_2:3163021-3163040	Msa0049690:CDS	60.0%
	ACCGCGACCTCTCTTGCAGT+GGG	-	chr1_2:3163025-3163044	None:intergenic	60.0%
	CTCGGAAGACACCGTTTCGC+CGG	+	chr1_2:3163126-3163145	Msa0049690:CDS	60.0%
	AGGATCCGTGTGAAGGGAGG+AGG	+	chr1_2:3163159-3163178	Msa0049690:CDS	60.0%
	GACCGCGACCTCTCTTGCAG+TGG	-	chr1_2:3163026-3163045	None:intergenic	65.0%
	GTGGTCGTGGTGCTCGTGCT+AGG	+	chr1_2:3163352-3163371	Msa0049690:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	3162973	3163395	3162973	ID=Msa0049690;Name=Msa0049690
chr1_2	mRNA	3162973	3163395	3162973	ID=Msa0049690-mRNA-1;Parent=Msa0049690;Name=Msa0049690-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|140
chr1_2	exon	3162973	3163395	3162973	ID=Msa0049690-mRNA-1:exon:732;Parent=Msa0049690-mRNA-1
chr1_2	CDS	3162973	3163395	3162973	ID=Msa0049690-mRNA-1:cds;Parent=Msa0049690-mRNA-1

Gene Sequence

>Msa0049690

ATGGAACAAGTGCAATGCTTTGGCCGGAAGAAGAACGCCGTTGCGGTAACCCACTGCAAGAGAGGTCGCGGTCTGATCAAGATCAACGGCTGTCCAATCGAACTGGTTGAGCCTGAGATTCTTCGTTTCAAGGCATACGAACCAATCCTCCTTCTCGGAAGACACCGTTTCGCCGGCGTTGATATGAGGATCCGTGTGAAGGGAGGAGGTCACACTTCTCAGATCTACGCCATTCGTCAGAGTATTGCTAAGGCTTTGGTTGCTTACTATCAGAAATTTGTTGATGAACAGAGTAAGAAAGAGATCAAGGATATTCTTGTTCGTTATGATCGTACTCTTCTTGTTGCTGATCCTAGACGCTGTGAGCCTAAGAAGTTTGGTGGTCGTGGTGCTCGTGCTAGGTTCCAGAAATCTTATCGTTAG

Protein sequence

>Msa0049690

MEQVQCFGRKKNAVAVTHCKRGRGLIKINGCPIELVEPEILRFKAYEPILLLGRHRFAGVDMRIRVKGGGHTSQIYAIRQSIAKALVAYYQKFVDEQSKKEIKDILVRYDRTLLVADPRRCEPKKFGGRGARARFQKSYR*