AlfalfaGEDB | Msa1437280

M. sativa cultivar zhongmu-4 / Msa1437280

KEGG: K02960
GO: GO:0000462 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005840 GO:0005844 GO:0005911 GO:0006139 GO:0006364 GO:0006396 GO:0006412 GO:0006518 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0015935 GO:0016020 GO:0016070 GO:0016072 GO:0019538 GO:0022613 GO:0022626 GO:0022627 GO:0030054 GO:0030312 GO:0030490 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034645 GO:0034660 GO:0042254 GO:0042274 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046483 GO:0055044 GO:0070013 GO:0071704 GO:0071840 GO:0071944 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00380.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1437280	XP_003631001.1	99.286	140	1	0	1	140	1	140	2.61e-96	283

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1437280	sp\|P46293\|RS16_GOSHI	93.571	140	9	0	1	140	6	145	1.09e-92	267

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1437280	G7LBJ0	99.286	140	1	0	1	140	1	140	1.25e-96	283

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0049690	Msa1437280	0.998282	9.655506e-261	-8.615850e-47
Msa1349570	Msa1437280	0.812568	3.971775e-51	-8.615850e-47
Msa1356790	Msa1437280	0.836369	9.744585e-57	-8.615850e-47
Msa1362790	Msa1437280	0.825150	5.484826e-54	-8.615850e-47
Msa1368920	Msa1437280	0.814400	1.570614e-51	-8.615850e-47
Msa1369190	Msa1437280	0.828912	6.906753e-55	-8.615850e-47
Msa1374240	Msa1437280	0.815758	7.846239e-52	-8.615850e-47
Msa1426120	Msa1437280	0.819182	1.328128e-52	-8.615850e-47
Msa1426490	Msa1437280	0.807726	4.390266e-50	-8.615850e-47
Msa1437280	Msa1450280	0.801937	7.114542e-49	-8.615850e-47
Msa1437280	Msa1456050	0.822259	2.606500e-53	-8.615850e-47
Msa0604960	Msa1437280	0.806662	7.377476e-50	-8.615850e-47
Msa0625570	Msa1437280	0.997014	1.474602e-235	-8.615850e-47
Msa0631250	Msa1437280	0.808224	3.440607e-50	-8.615850e-47
Msa0712610	Msa1437280	0.823205	1.570052e-53	-8.615850e-47
Msa0729200	Msa1437280	0.818890	1.547738e-52	-8.615850e-47
Msa0769590	Msa1437280	0.812972	3.239483e-51	-8.615850e-47
Msa0792420	Msa1437280	0.844589	6.883493e-59	-8.615850e-47
Msa0806880	Msa1437280	0.801080	1.065814e-48	-8.615850e-47
Msa0221030	Msa1437280	0.819768	9.765082e-53	-8.615850e-47
Msa1256840	Msa1437280	0.841989	3.400956e-58	-8.615850e-47
Msa1311400	Msa1437280	0.824675	7.099186e-54	-8.615850e-47
Msa1321790	Msa1437280	0.856500	3.085081e-62	-8.615850e-47
Msa1329300	Msa1437280	0.800569	1.355415e-48	-8.615850e-47
Msa1332250	Msa1437280	0.818560	1.839097e-52	-8.615850e-47
Msa1338190	Msa1437280	0.831109	2.010633e-55	-8.615850e-47
Msa0443390	Msa1437280	0.810979	8.802634e-51	-8.615850e-47
Msa1019260	Msa1437280	0.802532	5.364649e-49	-8.615850e-47
Msa1037290	Msa1437280	0.817265	3.606199e-52	-8.615850e-47
Msa1039520	Msa1437280	0.811716	6.090845e-51	-8.615850e-47
Msa1078870	Msa1437280	0.822292	2.561286e-53	-8.615850e-47
Msa1080350	Msa1437280	0.812434	4.247807e-51	-8.615850e-47
Msa0257450	Msa1437280	0.812393	4.337549e-51	-8.615850e-47
Msa0269180	Msa1437280	0.813875	2.051159e-51	-8.615850e-47
Msa0297830	Msa1437280	0.817728	2.837348e-52	-8.615850e-47
Msa0300350	Msa1437280	0.805369	1.380375e-49	-8.615850e-47
Msa0310080	Msa1437280	0.836850	7.348318e-57	-8.615850e-47
Msa0335840	Msa1437280	0.826117	3.234889e-54	-8.615850e-47
Msa1125860	Msa1437280	0.806796	6.913653e-50	-8.615850e-47
Msa1164150	Msa1437280	0.801494	8.767212e-49	-8.615850e-47
Msa0534750	Msa1437280	0.800884	1.169041e-48	-8.615850e-47
Msa0584030	Msa1437280	0.817983	2.484185e-52	-8.615850e-47
Msa0590940	Msa1437280	0.822641	2.125180e-53	-8.615850e-47
Msa0888810	Msa1437280	0.831112	2.007771e-55	-8.615850e-47
Msa0915540	Msa1437280	0.808853	2.524616e-50	-8.615850e-47
Msa0945480	Msa1437280	0.836732	7.877482e-57	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa1437280	MtrunA17_Chr8g0392251	99.286	140	1	1	140	1	140	2.40e-100	283
Msa1437280	MtrunA17_Chr8g0392191	99.286	140	1	1	140	1	140	2.40e-100	283
Msa1437280	MtrunA17_Chr5g0432551	98.571	140	2	1	140	1	140	6.11e-100	282
Msa1437280	MtrunA17_Chr4g0044571	94.286	140	8	1	140	4	143	7.79e-96	272

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa1437280	AT2G09990.1	89.209	139	15	2	140	8	146	1.92e-91	261
Msa1437280	AT5G18380.1	88.489	139	16	2	140	8	146	3.25e-91	261
Msa1437280	AT3G04230.1	83.453	139	23	2	140	8	146	6.19e-85	245
Msa1437280	AT5G18380.2	86.400	125	17	2	126	8	132	1.67e-78	229
Msa1437280	AT5G18380.3	86.111	108	15	2	109	8	115	5.55e-67	199

Find 38 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCTAACGATAAGATTTC+TGG	0.210396	tig0028256:-34308	None:intergenic
CCGCAACGGCGTTCTTCTTC+CGG	0.308388	tig0028256:-33927	None:intergenic
GCCTGAGATTCTTCGTTTCA+AGG	0.344740	tig0028256:+34014	Msa1437280:CDS
CGCTGTGAGCCTAAGAAGTT+TGG	0.359075	tig0028256:+34261	Msa1437280:CDS
TCAGAGTATTGCTAAGGCTT+TGG	0.386737	tig0028256:+34140	Msa1437280:CDS
CCACGACCACCAAACTTCTT+AGG	0.436009	tig0028256:-34270	None:intergenic
TACGAACCAATCCTCCTTCT+CGG	0.444217	tig0028256:+34039	Msa1437280:CDS
GTTTCGCCGGCGTTGATATG+AGG	0.478501	tig0028256:+34070	Msa1437280:CDS
GCCTTGAAACGAAGAATCTC+AGG	0.478888	tig0028256:-34015	None:intergenic
CTCGGAAGACACCGTTTCGC+CGG	0.484088	tig0028256:+34057	Msa1437280:CDS
ATGGAACAAGTGCAATGCTT+TGG	0.487256	tig0028256:+33904	Msa1437280:CDS
CAACGGCTGTCCAATCGAAC+TGG	0.488470	tig0028256:+33987	Msa1437280:CDS
GTGGTCGTGGTGCTCGTGCT+AGG	0.502073	tig0028256:+34283	Msa1437280:CDS
ACAGAGTAAGAAAGAGATCA+AGG	0.505686	tig0028256:+34191	Msa1437280:CDS
CCTAAGAAGTTTGGTGGTCG+TGG	0.511400	tig0028256:+34270	Msa1437280:CDS
AGGATCCGTGTGAAGGGAGG+AGG	0.521481	tig0028256:+34090	Msa1437280:CDS
TCATATCAACGCCGGCGAAA+CGG	0.524265	tig0028256:-34068	None:intergenic
TCAGGCTCAACCAGTTCGAT+TGG	0.524396	tig0028256:-33997	None:intergenic
AACAAGTGCAATGCTTTGGC+CGG	0.546867	tig0028256:+33908	Msa1437280:CDS
AACGGTGTCTTCCGAGAAGG+AGG	0.549270	tig0028256:-34050	None:intergenic
TGTGAGCCTAAGAAGTTTGG+TGG	0.558448	tig0028256:+34264	Msa1437280:CDS
GACCGCGACCTCTCTTGCAG+TGG	0.558828	tig0028256:-33954	None:intergenic
ACCCACTGCAAGAGAGGTCG+CGG	0.562945	tig0028256:+33952	Msa1437280:CDS
CATTCGTCAGAGTATTGCTA+AGG	0.576627	tig0028256:+34134	Msa1437280:CDS
TGATATGAGGATCCGTGTGA+AGG	0.579448	tig0028256:+34083	Msa1437280:CDS
GCGGTAACCCACTGCAAGAG+AGG	0.580471	tig0028256:+33946	Msa1437280:CDS
TTCTTAGGCTCACAGCGTCT+AGG	0.590494	tig0028256:-34255	None:intergenic
TGTCTTCCGAGAAGGAGGAT+TGG	0.592227	tig0028256:-34045	None:intergenic
GATATGAGGATCCGTGTGAA+GGG	0.594162	tig0028256:+34084	Msa1437280:CDS
CTTGCAGTGGGTTACCGCAA+CGG	0.599991	tig0028256:-33941	None:intergenic
ACCGCGACCTCTCTTGCAGT+GGG	0.603590	tig0028256:-33953	None:intergenic
CGCGGTCTGATCAAGATCAA+CGG	0.609302	tig0028256:+33970	Msa1437280:CDS
CTTAGCAATACTCTGACGAA+TGG	0.614188	tig0028256:-34133	None:intergenic
ATGAGGATCCGTGTGAAGGG+AGG	0.615322	tig0028256:+34087	Msa1437280:CDS
CGAAACGGTGTCTTCCGAGA+AGG	0.622095	tig0028256:-34053	None:intergenic
TGTGACCTCCTCCCTTCACA+CGG	0.629480	tig0028256:-34095	None:intergenic
CCGGAAGAAGAACGCCGTTG+CGG	0.651449	tig0028256:+33927	Msa1437280:CDS
ACGGATCCTCATATCAACGC+CGG	0.685813	tig0028256:-34076	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACAGAGTAAGAAAGAGATCA+AGG	+	tig0028256:34191-34210	Msa1437280:CDS	35.0%
	ATGGAACAAGTGCAATGCTT+TGG	+	tig0028256:33904-33923	Msa1437280:CDS	40.0%
	CTTAGCAATACTCTGACGAA+TGG	-	tig0028256:34136-34155	None:intergenic	40.0%
!!	CATTCGTCAGAGTATTGCTA+AGG	+	tig0028256:34134-34153	Msa1437280:CDS	40.0%
!!	TCAGAGTATTGCTAAGGCTT+TGG	+	tig0028256:34140-34159	Msa1437280:CDS	40.0%
	AACAAGTGCAATGCTTTGGC+CGG	+	tig0028256:33908-33927	Msa1437280:CDS	45.0%
	GCCTGAGATTCTTCGTTTCA+AGG	+	tig0028256:34014-34033	Msa1437280:CDS	45.0%
	GCCTTGAAACGAAGAATCTC+AGG	-	tig0028256:34018-34037	None:intergenic	45.0%
	TACGAACCAATCCTCCTTCT+CGG	+	tig0028256:34039-34058	Msa1437280:CDS	45.0%
	TGATATGAGGATCCGTGTGA+AGG	+	tig0028256:34083-34102	Msa1437280:CDS	45.0%
	GATATGAGGATCCGTGTGAA+GGG	+	tig0028256:34084-34103	Msa1437280:CDS	45.0%
	TGTGAGCCTAAGAAGTTTGG+TGG	+	tig0028256:34264-34283	Msa1437280:CDS	45.0%
	CGCGGTCTGATCAAGATCAA+CGG	+	tig0028256:33970-33989	Msa1437280:CDS	50.0%
	TCAGGCTCAACCAGTTCGAT+TGG	-	tig0028256:34000-34019	None:intergenic	50.0%
	TGTCTTCCGAGAAGGAGGAT+TGG	-	tig0028256:34048-34067	None:intergenic	50.0%
	TCATATCAACGCCGGCGAAA+CGG	-	tig0028256:34071-34090	None:intergenic	50.0%
	ACGGATCCTCATATCAACGC+CGG	-	tig0028256:34079-34098	None:intergenic	50.0%
	TTCTTAGGCTCACAGCGTCT+AGG	-	tig0028256:34258-34277	None:intergenic	50.0%
	CGCTGTGAGCCTAAGAAGTT+TGG	+	tig0028256:34261-34280	Msa1437280:CDS	50.0%
	CCACGACCACCAAACTTCTT+AGG	-	tig0028256:34273-34292	None:intergenic	50.0%
!	CCTAAGAAGTTTGGTGGTCG+TGG	+	tig0028256:34270-34289	Msa1437280:CDS	50.0%
!!	CTTGCAGTGGGTTACCGCAA+CGG	-	tig0028256:33944-33963	None:intergenic	55.0%
	CAACGGCTGTCCAATCGAAC+TGG	+	tig0028256:33987-34006	Msa1437280:CDS	55.0%
	AACGGTGTCTTCCGAGAAGG+AGG	-	tig0028256:34053-34072	None:intergenic	55.0%
	CGAAACGGTGTCTTCCGAGA+AGG	-	tig0028256:34056-34075	None:intergenic	55.0%
!!	GTTTCGCCGGCGTTGATATG+AGG	+	tig0028256:34070-34089	Msa1437280:CDS	55.0%
	ATGAGGATCCGTGTGAAGGG+AGG	+	tig0028256:34087-34106	Msa1437280:CDS	55.0%
	TGTGACCTCCTCCCTTCACA+CGG	-	tig0028256:34098-34117	None:intergenic	55.0%
	CCGCAACGGCGTTCTTCTTC+CGG	-	tig0028256:33930-33949	None:intergenic	60.0%
	CCGGAAGAAGAACGCCGTTG+CGG	+	tig0028256:33927-33946	Msa1437280:CDS	60.0%
	GCGGTAACCCACTGCAAGAG+AGG	+	tig0028256:33946-33965	Msa1437280:CDS	60.0%
	ACCCACTGCAAGAGAGGTCG+CGG	+	tig0028256:33952-33971	Msa1437280:CDS	60.0%
	ACCGCGACCTCTCTTGCAGT+GGG	-	tig0028256:33956-33975	None:intergenic	60.0%
	CTCGGAAGACACCGTTTCGC+CGG	+	tig0028256:34057-34076	Msa1437280:CDS	60.0%
	AGGATCCGTGTGAAGGGAGG+AGG	+	tig0028256:34090-34109	Msa1437280:CDS	60.0%
	GACCGCGACCTCTCTTGCAG+TGG	-	tig0028256:33957-33976	None:intergenic	65.0%
	GTGGTCGTGGTGCTCGTGCT+AGG	+	tig0028256:34283-34302	Msa1437280:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
tig0028256	gene	33904	34326	33904	ID=Msa1437280;Name=Msa1437280
tig0028256	mRNA	33904	34326	33904	ID=Msa1437280-mRNA-1;Parent=Msa1437280;Name=Msa1437280-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|140
tig0028256	exon	33904	34326	33904	ID=Msa1437280-mRNA-1:exon:10599;Parent=Msa1437280-mRNA-1
tig0028256	CDS	33904	34326	33904	ID=Msa1437280-mRNA-1:cds;Parent=Msa1437280-mRNA-1

Gene Sequence

>Msa1437280

ATGGAACAAGTGCAATGCTTTGGCCGGAAGAAGAACGCCGTTGCGGTAACCCACTGCAAGAGAGGTCGCGGTCTGATCAAGATCAACGGCTGTCCAATCGAACTGGTTGAGCCTGAGATTCTTCGTTTCAAGGCATACGAACCAATCCTCCTTCTCGGAAGACACCGTTTCGCCGGCGTTGATATGAGGATCCGTGTGAAGGGAGGAGGTCACACTTCTCAGATCTACGCCATTCGTCAGAGTATTGCTAAGGCTTTGGTTGCTTACTATCAGAAATTTGTTGATGAACAGAGTAAGAAAGAGATCAAGGATATTCTTGTTCGTTATGATCGTACTCTTCTTGTTGCTGATCCTAGACGCTGTGAGCCTAAGAAGTTTGGTGGTCGTGGTGCTCGTGCTAGGTTCCAGAAATCTTATCGTTAG

Protein sequence

>Msa1437280

MEQVQCFGRKKNAVAVTHCKRGRGLIKINGCPIELVEPEILRFKAYEPILLLGRHRFAGVDMRIRVKGGGHTSQIYAIRQSIAKALVAYYQKFVDEQSKKEIKDILVRYDRTLLVADPRRCEPKKFGGRGARARFQKSYR*