AlfalfaGEDB | Msa0625570

M. sativa cultivar zhongmu-4 / Msa0625570

KEGG: K02960
GO: GO:0000462 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005840 GO:0005844 GO:0005911 GO:0006139 GO:0006364 GO:0006396 GO:0006412 GO:0006518 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0015935 GO:0016020 GO:0016070 GO:0016072 GO:0019538 GO:0022613 GO:0022626 GO:0022627 GO:0030054 GO:0030312 GO:0030490 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034645 GO:0034660 GO:0042254 GO:0042274 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046483 GO:0055044 GO:0070013 GO:0071704 GO:0071840 GO:0071944 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00380.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0625570	XP_003631001.1	99.286	140	1	0	1	140	1	140	2.61e-96	283

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0625570	sp\|P46293\|RS16_GOSHI	93.571	140	9	0	1	140	6	145	1.09e-92	267

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0625570	G7LBJ0	99.286	140	1	0	1	140	1	140	1.25e-96	283

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0049690	Msa0625570	0.997535	2.688922e-244	-8.615850e-47
Msa0604960	Msa0625570	0.802211	6.247297e-49	-8.615850e-47
Msa0625570	Msa0631250	0.807804	4.225674e-50	-8.615850e-47
Msa0625570	Msa0712610	0.812550	4.007190e-51	-8.615850e-47
Msa0625570	Msa0729200	0.814218	1.723545e-51	-8.615850e-47
Msa0625570	Msa0769590	0.808045	3.755996e-50	-8.615850e-47
Msa0625570	Msa0792420	0.828927	6.848800e-55	-8.615850e-47
Msa0625570	Msa0888810	0.827486	1.523613e-54	-8.615850e-47
Msa0625570	Msa0901360	0.800967	1.124215e-48	-8.615850e-47
Msa0625570	Msa0915540	0.803994	2.671851e-49	-8.615850e-47
Msa0625570	Msa0945480	0.828818	7.274991e-55	-8.615850e-47
Msa0625570	Msa1037290	0.814492	1.498777e-51	-8.615850e-47
Msa0625570	Msa1039520	0.807384	5.190444e-50	-8.615850e-47
Msa0625570	Msa1078870	0.813867	2.059040e-51	-8.615850e-47
Msa0625570	Msa1256840	0.832567	8.784242e-56	-8.615850e-47
Msa0625570	Msa1311400	0.816909	4.336610e-52	-8.615850e-47
Msa0625570	Msa1321790	0.842267	2.870462e-58	-8.615850e-47
Msa0625570	Msa1329300	0.800317	1.525418e-48	-8.615850e-47
Msa0625570	Msa1332250	0.809932	1.482079e-50	-8.615850e-47
Msa0625570	Msa1338190	0.815755	7.856390e-52	-8.615850e-47
Msa0625570	Msa1349570	0.803197	3.910046e-49	-8.615850e-47
Msa0625570	Msa1356790	0.826573	2.519321e-54	-8.615850e-47
Msa0625570	Msa1362790	0.813323	2.712517e-51	-8.615850e-47
Msa0625570	Msa1368920	0.811780	5.897950e-51	-8.615850e-47
Msa0625570	Msa1369190	0.822556	2.223408e-53	-8.615850e-47
Msa0625570	Msa1374240	0.813696	2.245912e-51	-8.615850e-47
Msa0625570	Msa1426120	0.811454	6.944227e-51	-8.615850e-47
Msa0625570	Msa1437280	0.997014	1.474602e-235	-8.615850e-47
Msa0625570	Msa1456050	0.810297	1.236423e-50	-8.615850e-47
Msa0221030	Msa0625570	0.807711	4.422847e-50	-8.615850e-47
Msa0443390	Msa0625570	0.804190	2.433340e-49	-8.615850e-47
Msa0257450	Msa0625570	0.802167	6.379141e-49	-8.615850e-47
Msa0269180	Msa0625570	0.814380	1.586808e-51	-8.615850e-47
Msa0297830	Msa0625570	0.815268	1.008584e-51	-8.615850e-47
Msa0300350	Msa0625570	0.801733	7.835097e-49	-8.615850e-47
Msa0310080	Msa0625570	0.839211	1.814276e-57	-8.615850e-47
Msa0335840	Msa0625570	0.822961	1.790225e-53	-8.615850e-47
Msa0584030	Msa0625570	0.810641	1.042126e-50	-8.615850e-47
Msa0590940	Msa0625570	0.811647	6.305857e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0625570	MtrunA17_Chr8g0392251	99.286	140	1	1	140	1	140	2.40e-100	283
Msa0625570	MtrunA17_Chr8g0392191	99.286	140	1	1	140	1	140	2.40e-100	283
Msa0625570	MtrunA17_Chr5g0432551	98.571	140	2	1	140	1	140	6.11e-100	282
Msa0625570	MtrunA17_Chr4g0044571	94.286	140	8	1	140	4	143	7.79e-96	272

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0625570	AT2G09990.1	89.209	139	15	2	140	8	146	1.92e-91	261
Msa0625570	AT5G18380.1	88.489	139	16	2	140	8	146	3.25e-91	261
Msa0625570	AT3G04230.1	83.453	139	23	2	140	8	146	6.19e-85	245
Msa0625570	AT5G18380.2	86.400	125	17	2	126	8	132	1.67e-78	229
Msa0625570	AT5G18380.3	86.111	108	15	2	109	8	115	5.55e-67	199

Find 38 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCTAACGATAAGATTTC+TGG	0.210396	4_3:-4721279	None:intergenic
CCGCAACGGCGTTCTTCTTC+CGG	0.308388	4_3:-4720898	None:intergenic
GCCTGAGATTCTTCGTTTCA+AGG	0.344740	4_3:+4720985	Msa0625570:CDS
CGCTGTGAGCCTAAGAAGTT+TGG	0.359075	4_3:+4721232	Msa0625570:CDS
TCAGAGTATTGCTAAGGCTT+TGG	0.386737	4_3:+4721111	Msa0625570:CDS
CCACGACCACCAAACTTCTT+AGG	0.436009	4_3:-4721241	None:intergenic
TACGAACCAATCCTCCTTCT+CGG	0.444217	4_3:+4721010	Msa0625570:CDS
GTTTCGCCGGCGTTGATATG+AGG	0.478501	4_3:+4721041	Msa0625570:CDS
GCCTTGAAACGAAGAATCTC+AGG	0.478888	4_3:-4720986	None:intergenic
CTCGGAAGACACCGTTTCGC+CGG	0.484088	4_3:+4721028	Msa0625570:CDS
ATGGAACAAGTGCAATGCTT+TGG	0.487256	4_3:+4720875	Msa0625570:CDS
CAACGGCTGTCCAATCGAAC+TGG	0.488470	4_3:+4720958	Msa0625570:CDS
GTGGTCGTGGTGCTCGTGCT+AGG	0.502073	4_3:+4721254	Msa0625570:CDS
ACAGAGTAAGAAAGAGATCA+AGG	0.505686	4_3:+4721162	Msa0625570:CDS
CCTAAGAAGTTTGGTGGTCG+TGG	0.511400	4_3:+4721241	Msa0625570:CDS
AGGATCCGTGTGAAGGGAGG+AGG	0.521481	4_3:+4721061	Msa0625570:CDS
TCATATCAACGCCGGCGAAA+CGG	0.524265	4_3:-4721039	None:intergenic
TCAGGCTCAACCAGTTCGAT+TGG	0.524396	4_3:-4720968	None:intergenic
AACAAGTGCAATGCTTTGGC+CGG	0.546867	4_3:+4720879	Msa0625570:CDS
AACGGTGTCTTCCGAGAAGG+AGG	0.549270	4_3:-4721021	None:intergenic
TGTGAGCCTAAGAAGTTTGG+TGG	0.558448	4_3:+4721235	Msa0625570:CDS
GACCGCGACCTCTCTTGCAG+TGG	0.558828	4_3:-4720925	None:intergenic
ACCCACTGCAAGAGAGGTCG+CGG	0.562945	4_3:+4720923	Msa0625570:CDS
CATTCGTCAGAGTATTGCTA+AGG	0.576627	4_3:+4721105	Msa0625570:CDS
TGATATGAGGATCCGTGTGA+AGG	0.579448	4_3:+4721054	Msa0625570:CDS
GCGGTAACCCACTGCAAGAG+AGG	0.580471	4_3:+4720917	Msa0625570:CDS
TTCTTAGGCTCACAGCGTCT+AGG	0.590494	4_3:-4721226	None:intergenic
TGTCTTCCGAGAAGGAGGAT+TGG	0.592227	4_3:-4721016	None:intergenic
GATATGAGGATCCGTGTGAA+GGG	0.594162	4_3:+4721055	Msa0625570:CDS
CTTGCAGTGGGTTACCGCAA+CGG	0.599991	4_3:-4720912	None:intergenic
ACCGCGACCTCTCTTGCAGT+GGG	0.603590	4_3:-4720924	None:intergenic
CGCGGTCTGATCAAGATCAA+CGG	0.609302	4_3:+4720941	Msa0625570:CDS
CTTAGCAATACTCTGACGAA+TGG	0.614188	4_3:-4721104	None:intergenic
ATGAGGATCCGTGTGAAGGG+AGG	0.615322	4_3:+4721058	Msa0625570:CDS
CGAAACGGTGTCTTCCGAGA+AGG	0.622095	4_3:-4721024	None:intergenic
TGTGACCTCCTCCCTTCACA+CGG	0.629480	4_3:-4721066	None:intergenic
CCGGAAGAAGAACGCCGTTG+CGG	0.651449	4_3:+4720898	Msa0625570:CDS
ACGGATCCTCATATCAACGC+CGG	0.685813	4_3:-4721047	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACAGAGTAAGAAAGAGATCA+AGG	+	chr4_3:4721162-4721181	Msa0625570:CDS	35.0%
	ATGGAACAAGTGCAATGCTT+TGG	+	chr4_3:4720875-4720894	Msa0625570:CDS	40.0%
	CTTAGCAATACTCTGACGAA+TGG	-	chr4_3:4721107-4721126	None:intergenic	40.0%
!!	CATTCGTCAGAGTATTGCTA+AGG	+	chr4_3:4721105-4721124	Msa0625570:CDS	40.0%
!!	TCAGAGTATTGCTAAGGCTT+TGG	+	chr4_3:4721111-4721130	Msa0625570:CDS	40.0%
	AACAAGTGCAATGCTTTGGC+CGG	+	chr4_3:4720879-4720898	Msa0625570:CDS	45.0%
	GCCTGAGATTCTTCGTTTCA+AGG	+	chr4_3:4720985-4721004	Msa0625570:CDS	45.0%
	GCCTTGAAACGAAGAATCTC+AGG	-	chr4_3:4720989-4721008	None:intergenic	45.0%
	TACGAACCAATCCTCCTTCT+CGG	+	chr4_3:4721010-4721029	Msa0625570:CDS	45.0%
	TGATATGAGGATCCGTGTGA+AGG	+	chr4_3:4721054-4721073	Msa0625570:CDS	45.0%
	GATATGAGGATCCGTGTGAA+GGG	+	chr4_3:4721055-4721074	Msa0625570:CDS	45.0%
	TGTGAGCCTAAGAAGTTTGG+TGG	+	chr4_3:4721235-4721254	Msa0625570:CDS	45.0%
	CGCGGTCTGATCAAGATCAA+CGG	+	chr4_3:4720941-4720960	Msa0625570:CDS	50.0%
	TCAGGCTCAACCAGTTCGAT+TGG	-	chr4_3:4720971-4720990	None:intergenic	50.0%
	TGTCTTCCGAGAAGGAGGAT+TGG	-	chr4_3:4721019-4721038	None:intergenic	50.0%
	TCATATCAACGCCGGCGAAA+CGG	-	chr4_3:4721042-4721061	None:intergenic	50.0%
	ACGGATCCTCATATCAACGC+CGG	-	chr4_3:4721050-4721069	None:intergenic	50.0%
	TTCTTAGGCTCACAGCGTCT+AGG	-	chr4_3:4721229-4721248	None:intergenic	50.0%
	CGCTGTGAGCCTAAGAAGTT+TGG	+	chr4_3:4721232-4721251	Msa0625570:CDS	50.0%
	CCACGACCACCAAACTTCTT+AGG	-	chr4_3:4721244-4721263	None:intergenic	50.0%
!	CCTAAGAAGTTTGGTGGTCG+TGG	+	chr4_3:4721241-4721260	Msa0625570:CDS	50.0%
!!	CTTGCAGTGGGTTACCGCAA+CGG	-	chr4_3:4720915-4720934	None:intergenic	55.0%
	CAACGGCTGTCCAATCGAAC+TGG	+	chr4_3:4720958-4720977	Msa0625570:CDS	55.0%
	AACGGTGTCTTCCGAGAAGG+AGG	-	chr4_3:4721024-4721043	None:intergenic	55.0%
	CGAAACGGTGTCTTCCGAGA+AGG	-	chr4_3:4721027-4721046	None:intergenic	55.0%
!!	GTTTCGCCGGCGTTGATATG+AGG	+	chr4_3:4721041-4721060	Msa0625570:CDS	55.0%
	ATGAGGATCCGTGTGAAGGG+AGG	+	chr4_3:4721058-4721077	Msa0625570:CDS	55.0%
	TGTGACCTCCTCCCTTCACA+CGG	-	chr4_3:4721069-4721088	None:intergenic	55.0%
	CCGCAACGGCGTTCTTCTTC+CGG	-	chr4_3:4720901-4720920	None:intergenic	60.0%
	CCGGAAGAAGAACGCCGTTG+CGG	+	chr4_3:4720898-4720917	Msa0625570:CDS	60.0%
	GCGGTAACCCACTGCAAGAG+AGG	+	chr4_3:4720917-4720936	Msa0625570:CDS	60.0%
	ACCCACTGCAAGAGAGGTCG+CGG	+	chr4_3:4720923-4720942	Msa0625570:CDS	60.0%
	ACCGCGACCTCTCTTGCAGT+GGG	-	chr4_3:4720927-4720946	None:intergenic	60.0%
	CTCGGAAGACACCGTTTCGC+CGG	+	chr4_3:4721028-4721047	Msa0625570:CDS	60.0%
	AGGATCCGTGTGAAGGGAGG+AGG	+	chr4_3:4721061-4721080	Msa0625570:CDS	60.0%
	GACCGCGACCTCTCTTGCAG+TGG	-	chr4_3:4720928-4720947	None:intergenic	65.0%
	GTGGTCGTGGTGCTCGTGCT+AGG	+	chr4_3:4721254-4721273	Msa0625570:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	4720875	4721297	4720875	ID=Msa0625570;Name=Msa0625570
chr4_3	mRNA	4720875	4721297	4720875	ID=Msa0625570-mRNA-1;Parent=Msa0625570;Name=Msa0625570-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|140
chr4_3	exon	4720875	4721297	4720875	ID=Msa0625570-mRNA-1:exon:1304;Parent=Msa0625570-mRNA-1
chr4_3	CDS	4720875	4721297	4720875	ID=Msa0625570-mRNA-1:cds;Parent=Msa0625570-mRNA-1

Gene Sequence

>Msa0625570

ATGGAACAAGTGCAATGCTTTGGCCGGAAGAAGAACGCCGTTGCGGTAACCCACTGCAAGAGAGGTCGCGGTCTGATCAAGATCAACGGCTGTCCAATCGAACTGGTTGAGCCTGAGATTCTTCGTTTCAAGGCATACGAACCAATCCTCCTTCTCGGAAGACACCGTTTCGCCGGCGTTGATATGAGGATCCGTGTGAAGGGAGGAGGTCACACTTCTCAGATCTACGCCATTCGTCAGAGTATTGCTAAGGCTTTGGTTGCTTACTATCAGAAATTTGTTGATGAACAGAGTAAGAAAGAGATCAAGGATATTCTTGTTCGTTATGATCGTACTCTTCTTGTTGCTGATCCTAGACGCTGTGAGCCTAAGAAGTTTGGTGGTCGTGGTGCTCGTGCTAGGTTCCAGAAATCTTATCGTTAG

Protein sequence

>Msa0625570

MEQVQCFGRKKNAVAVTHCKRGRGLIKINGCPIELVEPEILRFKAYEPILLLGRHRFAGVDMRIRVKGGGHTSQIYAIRQSIAKALVAYYQKFVDEQSKKEIKDILVRYDRTLLVADPRRCEPKKFGGRGARARFQKSYR*