AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0310080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0310080 MtrunA17_Chr7g0263251 100.000 105 0 0 1 105 1 105 1.24e-71 208
Msa0310080 MtrunA17_Chr4g0050721 100.000 105 0 0 1 105 1 105 1.24e-71 208
Msa0310080 MtrunA17_Chr2g0278691 100.000 105 0 0 1 105 1 105 1.24e-71 208
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0310080 AT3G23390.1 92.381 105 8 0 1 105 1 105 1.05e-66 196
Msa0310080 AT4G14320.1 92.381 105 8 0 1 105 1 105 1.05e-66 196
Msa0310080 AT4G14320.2 91.398 93 8 0 1 93 1 93 3.44e-57 174

Find 58 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAAACTAGTGTGGAATTT+AGG 0.185779 2_4:-4931711 Msa0310080:three_prime_UTR
TCTCTTCTAGGTGGAGAATT+AGG 0.245518 2_4:-4932021 Msa0310080:exon
ATCTTTACCCTTCTTGTATT+GGG 0.247979 2_4:+4932887 None:intergenic
CCATTGTTGTTGAAATGTTT+TGG 0.281419 2_4:-4931973 Msa0310080:three_prime_UTR
TATCTTTACCCTTCTTGTAT+TGG 0.312780 2_4:+4932886 None:intergenic
AATGGTTTATGTGTATTGTT+GGG 0.345449 2_4:-4931592 Msa0310080:three_prime_UTR
CACATATGTTTGTAGGGAAA+TGG 0.350911 2_4:-4931610 Msa0310080:three_prime_UTR
TTGGGGAAATCAAGGTGATT+GGG 0.367665 2_4:-4931574 Msa0310080:three_prime_UTR
AAATGGTTTATGTGTATTGT+TGG 0.380502 2_4:-4931593 Msa0310080:three_prime_UTR
CAAGGGAACTTCTCTCTTCT+AGG 0.380957 2_4:-4932033 Msa0310080:exon
TGTTGAACACATATGTTTGT+AGG 0.381230 2_4:-4931617 Msa0310080:three_prime_UTR
GATCGCAAACAGTCTGGTTA+TGG 0.387570 2_4:-4932834 Msa0310080:CDS
TGTTCTGAGGTTGCAATGCC+AGG 0.411690 2_4:-4932244 Msa0310080:CDS
AAAATCATCAAAGGAATTCA+AGG 0.412835 2_4:+4931862 None:intergenic
AATGGAGCGATGAAGCAAAA+TGG 0.417462 2_4:+4933118 None:intergenic
CATGGTGACGGCGCGTGGAT+TGG 0.425073 2_4:+4933063 None:intergenic
TCATTTCCTCTAGTTTGTGC+TGG 0.426931 2_4:-4931774 Msa0310080:three_prime_UTR
GTTGGGGAAATCAAGGTGAT+TGG 0.436724 2_4:-4931575 Msa0310080:three_prime_UTR
GCATTCCTTGCTCTTGCAGT+AGG 0.441918 2_4:+4932935 None:intergenic
TTTAAGTTTCTGTGTTAATG+TGG 0.446453 2_4:-4931688 Msa0310080:three_prime_UTR
TGAGATTGGTGGTGATAAGA+AGG 0.451732 2_4:-4932057 Msa0310080:CDS
GAGAAACATGCTTGCAGCCC+TGG 0.453437 2_4:+4932226 None:intergenic
GGGAAAGTCGGTGATTGAAT+TGG 0.463252 2_4:-4931666 Msa0310080:three_prime_UTR
CGTTACGATCGCAAACAGTC+TGG 0.464861 2_4:-4932840 Msa0310080:CDS
TTAAGTTTCTGTGTTAATGT+GGG 0.465340 2_4:-4931687 Msa0310080:three_prime_UTR
TGTTTCTCAACATGCAATCA+AGG 0.468902 2_4:-4932211 Msa0310080:intron
GTTGAACACATATGTTTGTA+GGG 0.469153 2_4:-4931616 Msa0310080:three_prime_UTR
TGGGGAAATCAAGGTGATTG+GGG 0.489090 2_4:-4931573 Msa0310080:three_prime_UTR
GTTACCTTCTTGTGAAAGAC+GGG 0.495280 2_4:+4932798 None:intergenic
GTGTATTGTTGGGGAAATCA+AGG 0.511221 2_4:-4931582 Msa0310080:three_prime_UTR
GAGATTGGTGGTGATAAGAA+GGG 0.526133 2_4:-4932056 Msa0310080:CDS
CAAGGTTACCCAATACAAGA+AGG 0.553515 2_4:-4932895 Msa0310080:CDS
CGCAAACAGTCTGGTTATGG+TGG 0.554816 2_4:-4932831 Msa0310080:CDS
TGGTGGTGATAAGAAGGGCA+AGG 0.556388 2_4:-4932051 Msa0310080:CDS
CCAAAACATTTCAACAACAA+TGG 0.558543 2_4:+4931973 None:intergenic
TAAGTTTCTGTGTTAATGTG+GGG 0.560887 2_4:-4931686 Msa0310080:three_prime_UTR
TTTCAATTTACCGAACATTG+TGG 0.561609 2_4:+4931728 None:intergenic
GTTACCGTCTCCACAATGTT+CGG 0.561933 2_4:-4931738 Msa0310080:three_prime_UTR
ATTACCATGGTGACGGCGCG+TGG 0.569167 2_4:+4933058 None:intergenic
AAAGATAGCATCGCCGCTCA+AGG 0.571046 2_4:-4932870 Msa0310080:CDS
AGTTACCTTCTTGTGAAAGA+CGG 0.572609 2_4:+4932797 None:intergenic
CAAGAAGCATACACTTCACA+AGG 0.596444 2_4:-4932913 Msa0310080:CDS
TGTGTTAATGTGGGGAAAGT+CGG 0.596962 2_4:-4931678 Msa0310080:three_prime_UTR
TTTACCGAACATTGTGGAGA+CGG 0.600929 2_4:+4931734 None:intergenic
GAAAACCTACTGCAAGAGCA+AGG 0.601503 2_4:-4932940 Msa0310080:CDS
CAATCCACGCGCCGTCACCA+TGG 0.602128 2_4:-4933062 Msa0310080:intron
CCACTAAGAAAATTGTTCTG+AGG 0.602363 2_4:-4932257 Msa0310080:CDS
TAAGCCCGTCTTTCACAAGA+AGG 0.606865 2_4:-4932802 Msa0310080:intron
ATGGTTTATGTGTATTGTTG+GGG 0.618518 2_4:-4931591 Msa0310080:three_prime_UTR
TCGGTAAATTGAAACTAGTG+TGG 0.628118 2_4:-4931719 Msa0310080:three_prime_UTR
AAGGTTACCCAATACAAGAA+GGG 0.630634 2_4:-4932894 Msa0310080:CDS
GTTCTGAGGTTGCAATGCCA+GGG 0.630775 2_4:-4932243 Msa0310080:CDS
GGTGGTGATAAGAAGGGCAA+GGG 0.630826 2_4:-4932050 Msa0310080:CDS
GTAACGACGCTTACCTTGAG+CGG 0.638526 2_4:+4932857 None:intergenic
GGAGATGATTACCATGGTGA+CGG 0.643982 2_4:+4933051 None:intergenic
TTAACACCAGCACAAACTAG+AGG 0.663142 2_4:+4931768 None:intergenic
GGGAACTTCTCTCTTCTAGG+TGG 0.678633 2_4:-4932030 Msa0310080:exon
GCGCGTGGATTGGATCTGAG+AGG 0.710537 2_4:+4933073 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGATTTTTTTTTAATTGAA+TGG - chr2_4:4932379-4932398 Msa0310080:intron 10.0%
!!! TTGATTTTTTTTTAATTGAA+TGG - chr2_4:4932379-4932398 Msa0310080:intron 10.0%
!!! TGTATTTGATTTTATTGTAT+AGG - chr2_4:4932423-4932442 Msa0310080:intron 15.0%
!!! AAGATTTTTAAAATCATCAA+AGG + chr2_4:4932859-4932878 None:intergenic 15.0%
!!! TTTAAAAATCTTTTATCATC+TGG - chr2_4:4932867-4932886 Msa0310080:CDS 15.0%
!!! TTAAAAATCTTTTATCATCT+GGG - chr2_4:4932868-4932887 Msa0310080:CDS 15.0%
!!! ATCTAATTTTATCATAACAA+TGG + chr2_4:4932909-4932928 None:intergenic 15.0%
!!! TGTATTTGATTTTATTGTAT+AGG - chr2_4:4932423-4932442 Msa0310080:intron 15.0%
!!! AAGATTTTTAAAATCATCAA+AGG + chr2_4:4932859-4932878 None:intergenic 15.0%
!!! TTTAAAAATCTTTTATCATC+TGG - chr2_4:4932867-4932886 Msa0310080:CDS 15.0%
!!! TTAAAAATCTTTTATCATCT+GGG - chr2_4:4932868-4932887 Msa0310080:CDS 15.0%
!!! ATCTAATTTTATCATAACAA+TGG + chr2_4:4932909-4932928 None:intergenic 15.0%
!! AAATGTATAAAAGTAATGAG+GGG + chr2_4:4931949-4931968 None:intergenic 20.0%
!! GAAATGTATAAAAGTAATGA+GGG + chr2_4:4931950-4931969 None:intergenic 20.0%
!! TGAAATGTATAAAAGTAATG+AGG + chr2_4:4931951-4931970 None:intergenic 20.0%
!! CTTACAATCAACTAAAAAAA+TGG + chr2_4:4931993-4932012 None:intergenic 20.0%
!!! CAGAAATTTTATTTTGTAGA+TGG - chr2_4:4932561-4932580 Msa0310080:intron 20.0%
!!! TTTTATTTTGTAGATGGATA+TGG - chr2_4:4932567-4932586 Msa0310080:intron 20.0%
!! AAATGTATAAAAGTAATGAG+GGG + chr2_4:4931949-4931968 None:intergenic 20.0%
!! GAAATGTATAAAAGTAATGA+GGG + chr2_4:4931950-4931969 None:intergenic 20.0%
!! TGAAATGTATAAAAGTAATG+AGG + chr2_4:4931951-4931970 None:intergenic 20.0%
!! CTTACAATCAACTAAAAAAA+TGG + chr2_4:4931993-4932012 None:intergenic 20.0%
!!! CAGAAATTTTATTTTGTAGA+TGG - chr2_4:4932561-4932580 Msa0310080:intron 20.0%
!!! TTTTATTTTGTAGATGGATA+TGG - chr2_4:4932567-4932586 Msa0310080:intron 20.0%
! TGATATAATTACGATTACGT+AGG + chr2_4:4931696-4931715 None:intergenic 25.0%
! GAAAATCCAACATCAAAAAA+GGG + chr2_4:4931727-4931746 None:intergenic 25.0%
! TGAAAATCCAACATCAAAAA+AGG + chr2_4:4931728-4931747 None:intergenic 25.0%
!!! TTTTTTGATGTTGGATTTTC+AGG - chr2_4:4931727-4931746 Msa0310080:three_prime_UTR 25.0%
! AATGTATAAAAGTAATGAGG+GGG + chr2_4:4931948-4931967 None:intergenic 25.0%
!!! TTAGCTTTATGCAAATGATT+AGG - chr2_4:4932074-4932093 Msa0310080:CDS 25.0%
!!! TAGCTTTATGCAAATGATTA+GGG - chr2_4:4932075-4932094 Msa0310080:intron 25.0%
!!! TCAAGGTTAGTTTGTTTATT+TGG - chr2_4:4932515-4932534 Msa0310080:intron 25.0%
! ATATGGTTGTTTGTATCTAA+TGG - chr2_4:4932584-4932603 Msa0310080:intron 25.0%
! AAAATCATCAAAGGAATTCA+AGG + chr2_4:4932850-4932869 None:intergenic 25.0%
!! TTTAAGTTTCTGTGTTAATG+TGG - chr2_4:4933021-4933040 Msa0310080:intron 25.0%
! TTAAGTTTCTGTGTTAATGT+GGG - chr2_4:4933022-4933041 Msa0310080:intron 25.0%
! AAATGGTTTATGTGTATTGT+TGG - chr2_4:4933116-4933135 Msa0310080:five_prime_UTR 25.0%
! AATGGTTTATGTGTATTGTT+GGG - chr2_4:4933117-4933136 Msa0310080:five_prime_UTR 25.0%
! TGATATAATTACGATTACGT+AGG + chr2_4:4931696-4931715 None:intergenic 25.0%
! GAAAATCCAACATCAAAAAA+GGG + chr2_4:4931727-4931746 None:intergenic 25.0%
! TGAAAATCCAACATCAAAAA+AGG + chr2_4:4931728-4931747 None:intergenic 25.0%
!!! TTTTTTGATGTTGGATTTTC+AGG - chr2_4:4931727-4931746 Msa0310080:three_prime_UTR 25.0%
! AATGTATAAAAGTAATGAGG+GGG + chr2_4:4931948-4931967 None:intergenic 25.0%
!!! TTAGCTTTATGCAAATGATT+AGG - chr2_4:4932074-4932093 Msa0310080:CDS 25.0%
!!! TAGCTTTATGCAAATGATTA+GGG - chr2_4:4932075-4932094 Msa0310080:intron 25.0%
!!! TCAAGGTTAGTTTGTTTATT+TGG - chr2_4:4932515-4932534 Msa0310080:intron 25.0%
! ATATGGTTGTTTGTATCTAA+TGG - chr2_4:4932584-4932603 Msa0310080:intron 25.0%
! AAAATCATCAAAGGAATTCA+AGG + chr2_4:4932850-4932869 None:intergenic 25.0%
!! TTTAAGTTTCTGTGTTAATG+TGG - chr2_4:4933021-4933040 Msa0310080:intron 25.0%
! TTAAGTTTCTGTGTTAATGT+GGG - chr2_4:4933022-4933041 Msa0310080:intron 25.0%
! AAATGGTTTATGTGTATTGT+TGG - chr2_4:4933116-4933135 Msa0310080:five_prime_UTR 25.0%
! AATGGTTTATGTGTATTGTT+GGG - chr2_4:4933117-4933136 Msa0310080:five_prime_UTR 25.0%
!! GCAAAATGGTTTGTGTTTTA+TGG + chr2_4:4931580-4931599 None:intergenic 30.0%
ATCTTTACCCTTCTTGTATT+GGG + chr2_4:4931825-4931844 None:intergenic 30.0%
TATCTTTACCCTTCTTGTAT+TGG + chr2_4:4931826-4931845 None:intergenic 30.0%
AGGGAAGATTTAGAGATTAA+TGG - chr2_4:4932094-4932113 Msa0310080:intron 30.0%
GGGAAGATTTAGAGATTAAT+GGG - chr2_4:4932095-4932114 Msa0310080:intron 30.0%
! AGATTTAGAGATTAATGGGT+TGG - chr2_4:4932099-4932118 Msa0310080:intron 30.0%
! TTTTGAACTGAATGCGAAAT+TGG - chr2_4:4932217-4932236 Msa0310080:CDS 30.0%
CCAAAACATTTCAACAACAA+TGG + chr2_4:4932739-4932758 None:intergenic 30.0%
!! CCATTGTTGTTGAAATGTTT+TGG - chr2_4:4932736-4932755 Msa0310080:intron 30.0%
!!! TGAAATGTTTTGGTTCTCTA+TGG - chr2_4:4932746-4932765 Msa0310080:intron 30.0%
TTTCAATTTACCGAACATTG+TGG + chr2_4:4932984-4933003 None:intergenic 30.0%
TTGAAACTAGTGTGGAATTT+AGG - chr2_4:4932998-4933017 Msa0310080:intron 30.0%
TAAGTTTCTGTGTTAATGTG+GGG - chr2_4:4933023-4933042 Msa0310080:intron 30.0%
TGTTGAACACATATGTTTGT+AGG - chr2_4:4933092-4933111 Msa0310080:five_prime_UTR 30.0%
GTTGAACACATATGTTTGTA+GGG - chr2_4:4933093-4933112 Msa0310080:five_prime_UTR 30.0%
ATGGTTTATGTGTATTGTTG+GGG - chr2_4:4933118-4933137 Msa0310080:five_prime_UTR 30.0%
!! GCAAAATGGTTTGTGTTTTA+TGG + chr2_4:4931580-4931599 None:intergenic 30.0%
ATCTTTACCCTTCTTGTATT+GGG + chr2_4:4931825-4931844 None:intergenic 30.0%
TATCTTTACCCTTCTTGTAT+TGG + chr2_4:4931826-4931845 None:intergenic 30.0%
AGGGAAGATTTAGAGATTAA+TGG - chr2_4:4932094-4932113 Msa0310080:intron 30.0%
GGGAAGATTTAGAGATTAAT+GGG - chr2_4:4932095-4932114 Msa0310080:intron 30.0%
! AGATTTAGAGATTAATGGGT+TGG - chr2_4:4932099-4932118 Msa0310080:intron 30.0%
! TTTTGAACTGAATGCGAAAT+TGG - chr2_4:4932217-4932236 Msa0310080:CDS 30.0%
CCAAAACATTTCAACAACAA+TGG + chr2_4:4932739-4932758 None:intergenic 30.0%
!! CCATTGTTGTTGAAATGTTT+TGG - chr2_4:4932736-4932755 Msa0310080:intron 30.0%
!!! TGAAATGTTTTGGTTCTCTA+TGG - chr2_4:4932746-4932765 Msa0310080:intron 30.0%
TTTCAATTTACCGAACATTG+TGG + chr2_4:4932984-4933003 None:intergenic 30.0%
TTGAAACTAGTGTGGAATTT+AGG - chr2_4:4932998-4933017 Msa0310080:intron 30.0%
TAAGTTTCTGTGTTAATGTG+GGG - chr2_4:4933023-4933042 Msa0310080:intron 30.0%
TGTTGAACACATATGTTTGT+AGG - chr2_4:4933092-4933111 Msa0310080:five_prime_UTR 30.0%
GTTGAACACATATGTTTGTA+GGG - chr2_4:4933093-4933112 Msa0310080:five_prime_UTR 30.0%
ATGGTTTATGTGTATTGTTG+GGG - chr2_4:4933118-4933137 Msa0310080:five_prime_UTR 30.0%
!!! GCTAAACCCTTTTTTGATGT+TGG - chr2_4:4931718-4931737 Msa0310080:three_prime_UTR 35.0%
! CAGTAGGTTTTCTTTGTCTT+TGG + chr2_4:4931761-4931780 None:intergenic 35.0%
AAGGTTACCCAATACAAGAA+GGG - chr2_4:4931815-4931834 Msa0310080:three_prime_UTR 35.0%
AGTTACCTTCTTGTGAAAGA+CGG + chr2_4:4931915-4931934 None:intergenic 35.0%
! TGGGTTGGATTCATCAAATA+TGG - chr2_4:4932114-4932133 Msa0310080:intron 35.0%
! CCTCAGAACAATTTTCTTAG+TGG + chr2_4:4932455-4932474 None:intergenic 35.0%
CCACTAAGAAAATTGTTCTG+AGG - chr2_4:4932452-4932471 Msa0310080:intron 35.0%
TGTTTCTCAACATGCAATCA+AGG - chr2_4:4932498-4932517 Msa0310080:intron 35.0%
TCGGTAAATTGAAACTAGTG+TGG - chr2_4:4932990-4933009 Msa0310080:intron 35.0%
CACATATGTTTGTAGGGAAA+TGG - chr2_4:4933099-4933118 Msa0310080:five_prime_UTR 35.0%
!!! GCTAAACCCTTTTTTGATGT+TGG - chr2_4:4931718-4931737 Msa0310080:three_prime_UTR 35.0%
! CAGTAGGTTTTCTTTGTCTT+TGG + chr2_4:4931761-4931780 None:intergenic 35.0%
AAGGTTACCCAATACAAGAA+GGG - chr2_4:4931815-4931834 Msa0310080:three_prime_UTR 35.0%
AGTTACCTTCTTGTGAAAGA+CGG + chr2_4:4931915-4931934 None:intergenic 35.0%
! TGGGTTGGATTCATCAAATA+TGG - chr2_4:4932114-4932133 Msa0310080:intron 35.0%
! CCTCAGAACAATTTTCTTAG+TGG + chr2_4:4932455-4932474 None:intergenic 35.0%
CCACTAAGAAAATTGTTCTG+AGG - chr2_4:4932452-4932471 Msa0310080:intron 35.0%
TGTTTCTCAACATGCAATCA+AGG - chr2_4:4932498-4932517 Msa0310080:intron 35.0%
TCGGTAAATTGAAACTAGTG+TGG - chr2_4:4932990-4933009 Msa0310080:intron 35.0%
CACATATGTTTGTAGGGAAA+TGG - chr2_4:4933099-4933118 Msa0310080:five_prime_UTR 35.0%
AATGGAGCGATGAAGCAAAA+TGG + chr2_4:4931594-4931613 None:intergenic 40.0%
!! AGTTTTGTGCGCAGTGAAAA+TGG + chr2_4:4931612-4931631 None:intergenic 40.0%
! CAAGAAGCATACACTTCACA+AGG - chr2_4:4931796-4931815 Msa0310080:three_prime_UTR 40.0%
CAAGGTTACCCAATACAAGA+AGG - chr2_4:4931814-4931833 Msa0310080:three_prime_UTR 40.0%
GTTACCTTCTTGTGAAAGAC+GGG + chr2_4:4931914-4931933 None:intergenic 40.0%
! AAGAAGCCTTATACCACTGT+GGG + chr2_4:4932174-4932193 None:intergenic 40.0%
! GTTGTGTTTCTGTTATGCAG+AGG - chr2_4:4932618-4932637 Msa0310080:intron 40.0%
!!! AGGTGCAAGCATTTTGAGAT+TGG - chr2_4:4932638-4932657 Msa0310080:intron 40.0%
!!! TGCAAGCATTTTGAGATTGG+TGG - chr2_4:4932641-4932660 Msa0310080:intron 40.0%
!! TGAGATTGGTGGTGATAAGA+AGG - chr2_4:4932652-4932671 Msa0310080:intron 40.0%
!! GAGATTGGTGGTGATAAGAA+GGG - chr2_4:4932653-4932672 Msa0310080:intron 40.0%
TCTCTTCTAGGTGGAGAATT+AGG - chr2_4:4932688-4932707 Msa0310080:intron 40.0%
! TCATTTCCTCTAGTTTGTGC+TGG - chr2_4:4932935-4932954 Msa0310080:CDS 40.0%
TTAACACCAGCACAAACTAG+AGG + chr2_4:4932944-4932963 None:intergenic 40.0%
TTTACCGAACATTGTGGAGA+CGG + chr2_4:4932978-4932997 None:intergenic 40.0%
TGTGTTAATGTGGGGAAAGT+CGG - chr2_4:4933031-4933050 Msa0310080:intron 40.0%
GTGTATTGTTGGGGAAATCA+AGG - chr2_4:4933127-4933146 Msa0310080:five_prime_UTR 40.0%
TTGGGGAAATCAAGGTGATT+GGG - chr2_4:4933135-4933154 Msa0310080:five_prime_UTR 40.0%
AATGGAGCGATGAAGCAAAA+TGG + chr2_4:4931594-4931613 None:intergenic 40.0%
!! AGTTTTGTGCGCAGTGAAAA+TGG + chr2_4:4931612-4931631 None:intergenic 40.0%
! CAAGAAGCATACACTTCACA+AGG - chr2_4:4931796-4931815 Msa0310080:three_prime_UTR 40.0%
CAAGGTTACCCAATACAAGA+AGG - chr2_4:4931814-4931833 Msa0310080:three_prime_UTR 40.0%
GTTACCTTCTTGTGAAAGAC+GGG + chr2_4:4931914-4931933 None:intergenic 40.0%
! AAGAAGCCTTATACCACTGT+GGG + chr2_4:4932174-4932193 None:intergenic 40.0%
! GTTGTGTTTCTGTTATGCAG+AGG - chr2_4:4932618-4932637 Msa0310080:intron 40.0%
!!! AGGTGCAAGCATTTTGAGAT+TGG - chr2_4:4932638-4932657 Msa0310080:intron 40.0%
!!! TGCAAGCATTTTGAGATTGG+TGG - chr2_4:4932641-4932660 Msa0310080:intron 40.0%
!! TGAGATTGGTGGTGATAAGA+AGG - chr2_4:4932652-4932671 Msa0310080:intron 40.0%
!! GAGATTGGTGGTGATAAGAA+GGG - chr2_4:4932653-4932672 Msa0310080:intron 40.0%
TCTCTTCTAGGTGGAGAATT+AGG - chr2_4:4932688-4932707 Msa0310080:intron 40.0%
! TCATTTCCTCTAGTTTGTGC+TGG - chr2_4:4932935-4932954 Msa0310080:CDS 40.0%
TTAACACCAGCACAAACTAG+AGG + chr2_4:4932944-4932963 None:intergenic 40.0%
TTTACCGAACATTGTGGAGA+CGG + chr2_4:4932978-4932997 None:intergenic 40.0%
TGTGTTAATGTGGGGAAAGT+CGG - chr2_4:4933031-4933050 Msa0310080:intron 40.0%
GTGTATTGTTGGGGAAATCA+AGG - chr2_4:4933127-4933146 Msa0310080:five_prime_UTR 40.0%
TTGGGGAAATCAAGGTGATT+GGG - chr2_4:4933135-4933154 Msa0310080:five_prime_UTR 40.0%
GGAGATGATTACCATGGTGA+CGG + chr2_4:4931661-4931680 None:intergenic 45.0%
CGATTACGTAGGAACAGACA+CGG + chr2_4:4931685-4931704 None:intergenic 45.0%
GAAAACCTACTGCAAGAGCA+AGG - chr2_4:4931769-4931788 Msa0310080:three_prime_UTR 45.0%
GATCGCAAACAGTCTGGTTA+TGG - chr2_4:4931875-4931894 Msa0310080:three_prime_UTR 45.0%
TAAGCCCGTCTTTCACAAGA+AGG - chr2_4:4931907-4931926 Msa0310080:three_prime_UTR 45.0%
AAGTAATGAGGGGGTGAAGT+GGG + chr2_4:4931939-4931958 None:intergenic 45.0%
AAAGTAATGAGGGGGTGAAG+TGG + chr2_4:4931940-4931959 None:intergenic 45.0%
! CAAGAAGCCTTATACCACTG+TGG + chr2_4:4932175-4932194 None:intergenic 45.0%
CAAGGGAACTTCTCTCTTCT+AGG - chr2_4:4932676-4932695 Msa0310080:intron 45.0%
GTTACCGTCTCCACAATGTT+CGG - chr2_4:4932971-4932990 Msa0310080:intron 45.0%
GGGAAAGTCGGTGATTGAAT+TGG - chr2_4:4933043-4933062 Msa0310080:intron 45.0%
GTTGGGGAAATCAAGGTGAT+TGG - chr2_4:4933134-4933153 Msa0310080:five_prime_UTR 45.0%
TGGGGAAATCAAGGTGATTG+GGG - chr2_4:4933136-4933155 Msa0310080:five_prime_UTR 45.0%
GGAGATGATTACCATGGTGA+CGG + chr2_4:4931661-4931680 None:intergenic 45.0%
CGATTACGTAGGAACAGACA+CGG + chr2_4:4931685-4931704 None:intergenic 45.0%
GAAAACCTACTGCAAGAGCA+AGG - chr2_4:4931769-4931788 Msa0310080:three_prime_UTR 45.0%
GATCGCAAACAGTCTGGTTA+TGG - chr2_4:4931875-4931894 Msa0310080:three_prime_UTR 45.0%
TAAGCCCGTCTTTCACAAGA+AGG - chr2_4:4931907-4931926 Msa0310080:three_prime_UTR 45.0%
AAGTAATGAGGGGGTGAAGT+GGG + chr2_4:4931939-4931958 None:intergenic 45.0%
AAAGTAATGAGGGGGTGAAG+TGG + chr2_4:4931940-4931959 None:intergenic 45.0%
! CAAGAAGCCTTATACCACTG+TGG + chr2_4:4932175-4932194 None:intergenic 45.0%
CAAGGGAACTTCTCTCTTCT+AGG - chr2_4:4932676-4932695 Msa0310080:intron 45.0%
GTTACCGTCTCCACAATGTT+CGG - chr2_4:4932971-4932990 Msa0310080:intron 45.0%
GGGAAAGTCGGTGATTGAAT+TGG - chr2_4:4933043-4933062 Msa0310080:intron 45.0%
GTTGGGGAAATCAAGGTGAT+TGG - chr2_4:4933134-4933153 Msa0310080:five_prime_UTR 45.0%
CACGGTGGAGATGATTACCA+TGG + chr2_4:4931667-4931686 None:intergenic 50.0%
TTACGTAGGAACAGACACGG+TGG + chr2_4:4931682-4931701 None:intergenic 50.0%
GCATTCCTTGCTCTTGCAGT+AGG + chr2_4:4931777-4931796 None:intergenic 50.0%
AAAGATAGCATCGCCGCTCA+AGG - chr2_4:4931839-4931858 Msa0310080:three_prime_UTR 50.0%
GTAACGACGCTTACCTTGAG+CGG + chr2_4:4931855-4931874 None:intergenic 50.0%
CGTTACGATCGCAAACAGTC+TGG - chr2_4:4931869-4931888 Msa0310080:three_prime_UTR 50.0%
CGCAAACAGTCTGGTTATGG+TGG - chr2_4:4931878-4931897 Msa0310080:three_prime_UTR 50.0%
TCATGTAGCCAAACCCACAG+TGG - chr2_4:4932158-4932177 Msa0310080:intron 50.0%
GCCAAACCCACAGTGGTATA+AGG - chr2_4:4932165-4932184 Msa0310080:intron 50.0%
GCCTTATACCACTGTGGGTT+TGG + chr2_4:4932169-4932188 None:intergenic 50.0%
!! TGTTCTGAGGTTGCAATGCC+AGG - chr2_4:4932465-4932484 Msa0310080:intron 50.0%
!! GTTCTGAGGTTGCAATGCCA+GGG - chr2_4:4932466-4932485 Msa0310080:intron 50.0%
! TGGTGGTGATAAGAAGGGCA+AGG - chr2_4:4932658-4932677 Msa0310080:intron 50.0%
GGTGGTGATAAGAAGGGCAA+GGG - chr2_4:4932659-4932678 Msa0310080:intron 50.0%
GGGAACTTCTCTCTTCTAGG+TGG - chr2_4:4932679-4932698 Msa0310080:intron 50.0%
CACGGTGGAGATGATTACCA+TGG + chr2_4:4931667-4931686 None:intergenic 50.0%
TTACGTAGGAACAGACACGG+TGG + chr2_4:4931682-4931701 None:intergenic 50.0%
GCATTCCTTGCTCTTGCAGT+AGG + chr2_4:4931777-4931796 None:intergenic 50.0%
AAAGATAGCATCGCCGCTCA+AGG - chr2_4:4931839-4931858 Msa0310080:three_prime_UTR 50.0%
GTAACGACGCTTACCTTGAG+CGG + chr2_4:4931855-4931874 None:intergenic 50.0%
CGTTACGATCGCAAACAGTC+TGG - chr2_4:4931869-4931888 Msa0310080:three_prime_UTR 50.0%
CGCAAACAGTCTGGTTATGG+TGG - chr2_4:4931878-4931897 Msa0310080:three_prime_UTR 50.0%
TCATGTAGCCAAACCCACAG+TGG - chr2_4:4932158-4932177 Msa0310080:intron 50.0%
GCCAAACCCACAGTGGTATA+AGG - chr2_4:4932165-4932184 Msa0310080:intron 50.0%
GCCTTATACCACTGTGGGTT+TGG + chr2_4:4932169-4932188 None:intergenic 50.0%
!! TGTTCTGAGGTTGCAATGCC+AGG - chr2_4:4932465-4932484 Msa0310080:intron 50.0%
!! GTTCTGAGGTTGCAATGCCA+GGG - chr2_4:4932466-4932485 Msa0310080:intron 50.0%
! TGGTGGTGATAAGAAGGGCA+AGG - chr2_4:4932658-4932677 Msa0310080:intron 50.0%
GGTGGTGATAAGAAGGGCAA+GGG - chr2_4:4932659-4932678 Msa0310080:intron 50.0%
GGGAACTTCTCTCTTCTAGG+TGG - chr2_4:4932679-4932698 Msa0310080:intron 50.0%
GAGAAACATGCTTGCAGCCC+TGG + chr2_4:4932486-4932505 None:intergenic 55.0%
GAGAAACATGCTTGCAGCCC+TGG + chr2_4:4932486-4932505 None:intergenic 55.0%
!! GCGCGTGGATTGGATCTGAG+AGG + chr2_4:4931639-4931658 None:intergenic 60.0%
!! ATTACCATGGTGACGGCGCG+TGG + chr2_4:4931654-4931673 None:intergenic 60.0%
!! GCGCGTGGATTGGATCTGAG+AGG + chr2_4:4931639-4931658 None:intergenic 60.0%
!! ATTACCATGGTGACGGCGCG+TGG + chr2_4:4931654-4931673 None:intergenic 60.0%
!! CATGGTGACGGCGCGTGGAT+TGG + chr2_4:4931649-4931668 None:intergenic 65.0%
CAATCCACGCGCCGTCACCA+TGG - chr2_4:4931647-4931666 Msa0310080:three_prime_UTR 65.0%
!! CATGGTGACGGCGCGTGGAT+TGG + chr2_4:4931649-4931668 None:intergenic 65.0%
CAATCCACGCGCCGTCACCA+TGG - chr2_4:4931647-4931666 Msa0310080:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 4931573 4933158 4931573 ID=Msa0310080;Name=Msa0310080
chr2_4 mRNA 4931573 4933158 4931573 ID=Msa0310080-mRNA-1;Parent=Msa0310080;Name=Msa0310080-mRNA-1;_AED=0.05;_eAED=0.05;_QI=93|1|1|1|0.66|0.75|4|461|105
chr2_4 exon 4931573 4932093 4931573 ID=Msa0310080-mRNA-1:exon:1687;Parent=Msa0310080-mRNA-1
chr2_4 exon 4932212 4932286 4932212 ID=Msa0310080-mRNA-1:exon:1686;Parent=Msa0310080-mRNA-1
chr2_4 exon 4932803 4932982 4932803 ID=Msa0310080-mRNA-1:exon:1685;Parent=Msa0310080-mRNA-1
chr2_4 exon 4933063 4933158 4933063 ID=Msa0310080-mRNA-1:exon:1684;Parent=Msa0310080-mRNA-1
chr2_4 five_prime_UTR 4933066 4933158 4933066 ID=Msa0310080-mRNA-1:five_prime_utr;Parent=Msa0310080-mRNA-1
chr2_4 CDS 4933063 4933065 4933063 ID=Msa0310080-mRNA-1:cds;Parent=Msa0310080-mRNA-1
chr2_4 CDS 4932803 4932982 4932803 ID=Msa0310080-mRNA-1:cds;Parent=Msa0310080-mRNA-1
chr2_4 CDS 4932212 4932286 4932212 ID=Msa0310080-mRNA-1:cds;Parent=Msa0310080-mRNA-1
chr2_4 CDS 4932034 4932093 4932034 ID=Msa0310080-mRNA-1:cds;Parent=Msa0310080-mRNA-1
chr2_4 three_prime_UTR 4931573 4932033 4931573 ID=Msa0310080-mRNA-1:three_prime_utr;Parent=Msa0310080-mRNA-1
Gene Sequence

>Msa0310080

ATGGTGAACGTTCCAAAGACAAAGAAAACCTACTGCAAGAGCAAGGAATGCAAGAAGCATACACTTCACAAGGTTACCCAATACAAGAAGGGTAAAGATAGCATCGCCGCTCAAGGTAAGCGTCGTTACGATCGCAAACAGTCTGGTTATGGTGGTCAGACTAAGCCCGTCTTTCACAAGAAGGCAAAAACCACTAAGAAAATTGTTCTGAGGTTGCAATGCCAGGGCTGCAAGCATGTTTCTCAACATGCAATCAAGAGGTGCAAGCATTTTGAGATTGGTGGTGATAAGAAGGGCAAGGGAACTTCTCTCTTCTAG
Protein sequence

>Msa0310080

MVNVPKTKKTYCKSKECKKHTLHKVTQYKKGKDSIAAQGKRRYDRKQSGYGGQTKPVFHKKAKTTKKIVLRLQCQGCKHVSQHAIKRCKHFEIGGDKKGKGTSLF*