AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0199240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0199240 MtrunA17_Chr8g0381871 97.902 143 3 0 1 143 1 143 3.68e-94 289
Msa0199240 MtrunA17_Chr2g0296441 75.862 145 29 3 1 143 1 141 8.71e-67 216
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0199240 AT5G44635.1 70.139 144 42 1 1 143 1 144 2.54e-67 218
Msa0199240 AT5G44635.2 70.139 144 42 1 1 143 1 144 2.63e-67 218

Find 57 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAGGTCATTGGATGTTATT+TGG 0.220922 2_1:-18155108 Msa0199240:three_prime_UTR
GGAAGCATCCGGCGGTTATT+TGG 0.247769 2_1:-18156229 Msa0199240:CDS
GCAACATTGATATCCTTGTT+AGG 0.308947 2_1:+18155719 None:intergenic
TTCAACCGATTCAGCGAAAA+TGG 0.322211 2_1:-18156250 Msa0199240:exon
GTCTTGTAGGTTAAGGGAAT+TGG 0.342010 2_1:-18155526 Msa0199240:intron
CTCCATAACAAACCTCTTGC+AGG 0.345294 2_1:+18155772 None:intergenic
TGGCTCTCTGCCTAGGTCAT+TGG 0.347819 2_1:-18155119 Msa0199240:three_prime_UTR
GACAATTGGAATGTTATAGA+AGG 0.359993 2_1:+18155697 None:intergenic
TGCATACACCTCTTGACAAT+TGG 0.367245 2_1:+18155683 None:intergenic
TGGATCGCCAGTTTCAAATC+TGG 0.367808 2_1:-18156059 Msa0199240:intron
TTCGATGTCTTGTAGGTTAA+GGG 0.372915 2_1:-18155532 Msa0199240:intron
GGTAGGCTTGTATCTGTAAC+AGG 0.380034 2_1:-18155489 Msa0199240:CDS
AAGAGAGAAAATTTACTGAT+TGG 0.389776 2_1:-18155183 Msa0199240:three_prime_UTR
TGCCTGCAAGAGGTTTGTTA+TGG 0.403641 2_1:-18155774 Msa0199240:CDS
CTGTTGTTACATGTAGCATT+TGG 0.405390 2_1:+18155224 None:intergenic
CAACATTGATATCCTTGTTA+GGG 0.416801 2_1:+18155720 None:intergenic
TTGCAGGCATTCTGCAAGTA+AGG 0.418482 2_1:+18155788 None:intergenic
CTAGACACTTGAAAGTTCCT+TGG 0.427198 2_1:+18155427 None:intergenic
AAAATCAATGAACATGGTGT+TGG 0.455706 2_1:+18155980 None:intergenic
AAAGTTCCTTGGAGAAGCTC+AGG 0.467629 2_1:+18155438 None:intergenic
GTAGCATTTGGACAAATTGT+TGG 0.476872 2_1:+18155236 None:intergenic
CTATCTCTGATGAATATTTG+AGG 0.491103 2_1:-18155920 Msa0199240:intron
GGTTGATGAGAAAGCCGTTA+GGG 0.498327 2_1:-18156208 Msa0199240:CDS
AAAATTTACTGATTGGCAAA+GGG 0.499023 2_1:-18155176 Msa0199240:three_prime_UTR
TGGCTACATCAGAGATTGGT+AGG 0.504042 2_1:-18155506 Msa0199240:CDS
TCAAGTGTCTAGAATGTGGT+GGG 0.506639 2_1:-18155416 Msa0199240:three_prime_UTR
TGGTTGATGAGAAAGCCGTT+AGG 0.508472 2_1:-18156209 Msa0199240:CDS
GAAAATTTACTGATTGGCAA+AGG 0.509024 2_1:-18155177 Msa0199240:three_prime_UTR
CGGAAAACGACGTCGGAGCA+AGG 0.528567 2_1:+18156291 None:intergenic
AGAGCCAACAGGTATCTCAT+TGG 0.528774 2_1:+18155135 None:intergenic
GAATTGGCTACATCAGAGAT+TGG 0.532287 2_1:-18155510 Msa0199240:CDS
TTGGCAAAGGGTCAGAATGC+AGG 0.537253 2_1:-18155164 Msa0199240:three_prime_UTR
TACCTGTTGGCTCTCTGCCT+AGG 0.538550 2_1:-18155126 Msa0199240:three_prime_UTR
GACCTAGGCAGAGAGCCAAC+AGG 0.539412 2_1:+18155124 None:intergenic
TCAGCGAAAATGGAAGCATC+CGG 0.548544 2_1:-18156240 Msa0199240:exon
ACTTGCAGAATGCCTGCAAG+AGG 0.569621 2_1:-18155784 Msa0199240:CDS
CTGTAACAGGAGTGGTAACA+AGG 0.572546 2_1:-18155476 Msa0199240:CDS
CTCATCAACCAAATAACCGC+CGG 0.579562 2_1:+18156221 None:intergenic
AAGCTTCTCTGAAACCTAGC+CGG 0.579851 2_1:+18156271 None:intergenic
GTTTGACCTGAGCTTCTCCA+AGG 0.579931 2_1:-18155444 Msa0199240:exon
GGAAAACGACGTCGGAGCAA+GGG 0.580284 2_1:+18156292 None:intergenic
ATAACATTCCAATTGTCAAG+AGG 0.586980 2_1:-18155691 Msa0199240:intron
CAAATAACATCCAATGACCT+AGG 0.587771 2_1:+18155109 None:intergenic
TTCAAGTGTCTAGAATGTGG+TGG 0.616955 2_1:-18155417 Msa0199240:three_prime_UTR
ACGTCCAATGAGATACCTGT+TGG 0.635018 2_1:-18155139 Msa0199240:three_prime_UTR
CAAGTGTCTAGAATGTGGTG+GGG 0.638582 2_1:-18155415 Msa0199240:three_prime_UTR
CCAGTTTCAAATCTGGACAA+AGG 0.643556 2_1:-18156052 Msa0199240:intron
TTATCATCAGAAATGAATGT+CGG 0.648409 2_1:+18155743 None:intergenic
GCTTGTATCTGTAACAGGAG+TGG 0.649403 2_1:-18155484 Msa0199240:CDS
ACTTTCAAGTGTCTAGAATG+TGG 0.654394 2_1:-18155420 Msa0199240:three_prime_UTR
ACGTTCAAAATCAATGAACA+TGG 0.655310 2_1:+18155974 None:intergenic
TTCTGATGATAACCCTAACA+AGG 0.660674 2_1:-18155732 Msa0199240:CDS
AGTGGTAACAAGGACAAGTG+AGG 0.673592 2_1:-18155466 Msa0199240:CDS
GAATGAACTCTATTACGAAG+CGG 0.674567 2_1:-18156030 Msa0199240:CDS
GCGAAAATGGAAGCATCCGG+CGG 0.694331 2_1:-18156237 Msa0199240:exon
ACCTAGCCGGAAAACGACGT+CGG 0.701293 2_1:+18156284 None:intergenic
ACAGCAATTCAAGTATACTG+AGG 0.742350 2_1:-18155376 Msa0199240:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTCAATAAAAATCCAAT+TGG + chr2_1:18155496-18155515 None:intergenic 15.0%
!!! CAAAATAATACCAAATTTTA+GGG + chr2_1:18155526-18155545 None:intergenic 15.0%
!!! CTAAAATTTGGTATTATTTT+GGG - chr2_1:18155525-18155544 Msa0199240:intron 15.0%
!! TTAAACAAAAATCAATGAAA+CGG + chr2_1:18155744-18155763 None:intergenic 15.0%
!! AATTTCAATAAAAATCCAAT+TGG + chr2_1:18155496-18155515 None:intergenic 15.0%
!!! CAAAATAATACCAAATTTTA+GGG + chr2_1:18155526-18155545 None:intergenic 15.0%
!!! CTAAAATTTGGTATTATTTT+GGG - chr2_1:18155525-18155544 Msa0199240:intron 15.0%
!! TTAAACAAAAATCAATGAAA+CGG + chr2_1:18155744-18155763 None:intergenic 15.0%
!!! CCAAAATAATACCAAATTTT+AGG + chr2_1:18155527-18155546 None:intergenic 20.0%
!!! CCTAAAATTTGGTATTATTT+TGG - chr2_1:18155524-18155543 Msa0199240:intron 20.0%
!!! GTGTTTTTAATTGAAATTTG+AGG - chr2_1:18155765-18155784 Msa0199240:CDS 20.0%
!!! CCAAAATAATACCAAATTTT+AGG + chr2_1:18155527-18155546 None:intergenic 20.0%
!!! CCTAAAATTTGGTATTATTT+TGG - chr2_1:18155524-18155543 Msa0199240:intron 20.0%
!!! GTGTTTTTAATTGAAATTTG+AGG - chr2_1:18155765-18155784 Msa0199240:CDS 20.0%
!! CTTTCTTAGATTTTCTCAAA+AGG - chr2_1:18155204-18155223 Msa0199240:three_prime_UTR 25.0%
!!! CAATTGTATCTGTTTTGTTT+TGG - chr2_1:18155301-18155320 Msa0199240:intron 25.0%
!!! TGAGGTATTTTTTTTCCAAT+TGG - chr2_1:18155478-18155497 Msa0199240:CDS 25.0%
! ATTACTGAAACCCTAAAATT+TGG - chr2_1:18155513-18155532 Msa0199240:CDS 25.0%
! TTATCATCAGAAATGAATGT+CGG + chr2_1:18155640-18155659 None:intergenic 25.0%
! AATGAAACGGAAACAAAAAT+TGG + chr2_1:18155731-18155750 None:intergenic 25.0%
!! AATCAGTAAATTTTCTCTCT+TGG + chr2_1:18156198-18156217 None:intergenic 25.0%
! AAGAGAGAAAATTTACTGAT+TGG - chr2_1:18156197-18156216 Msa0199240:CDS 25.0%
!! AAAATTTACTGATTGGCAAA+GGG - chr2_1:18156204-18156223 Msa0199240:CDS 25.0%
!! CTTTCTTAGATTTTCTCAAA+AGG - chr2_1:18155204-18155223 Msa0199240:three_prime_UTR 25.0%
!!! CAATTGTATCTGTTTTGTTT+TGG - chr2_1:18155301-18155320 Msa0199240:intron 25.0%
!!! TGAGGTATTTTTTTTCCAAT+TGG - chr2_1:18155478-18155497 Msa0199240:CDS 25.0%
! ATTACTGAAACCCTAAAATT+TGG - chr2_1:18155513-18155532 Msa0199240:CDS 25.0%
! TTATCATCAGAAATGAATGT+CGG + chr2_1:18155640-18155659 None:intergenic 25.0%
! AATGAAACGGAAACAAAAAT+TGG + chr2_1:18155731-18155750 None:intergenic 25.0%
!! AATCAGTAAATTTTCTCTCT+TGG + chr2_1:18156198-18156217 None:intergenic 25.0%
! AAGAGAGAAAATTTACTGAT+TGG - chr2_1:18156197-18156216 Msa0199240:CDS 25.0%
!! AAAATTTACTGATTGGCAAA+GGG - chr2_1:18156204-18156223 Msa0199240:CDS 25.0%
AAAATCAATGAACATGGTGT+TGG + chr2_1:18155403-18155422 None:intergenic 30.0%
ACGTTCAAAATCAATGAACA+TGG + chr2_1:18155409-18155428 None:intergenic 30.0%
CTATCTCTGATGAATATTTG+AGG - chr2_1:18155460-18155479 Msa0199240:exon 30.0%
!! TTTGTGTTGTTGTTGATGAT+AGG - chr2_1:18155563-18155582 Msa0199240:intron 30.0%
CAACATTGATATCCTTGTTA+GGG + chr2_1:18155663-18155682 None:intergenic 30.0%
GACAATTGGAATGTTATAGA+AGG + chr2_1:18155686-18155705 None:intergenic 30.0%
ATAACATTCCAATTGTCAAG+AGG - chr2_1:18155689-18155708 Msa0199240:intron 30.0%
!! TGTAGTTGTGATTTAGTTTG+TGG - chr2_1:18155791-18155810 Msa0199240:CDS 30.0%
!! GTAGTTGTGATTTAGTTTGT+GGG - chr2_1:18155792-18155811 Msa0199240:CDS 30.0%
! GAAAATTTACTGATTGGCAA+AGG - chr2_1:18156203-18156222 Msa0199240:CDS 30.0%
AAAATCAATGAACATGGTGT+TGG + chr2_1:18155403-18155422 None:intergenic 30.0%
ACGTTCAAAATCAATGAACA+TGG + chr2_1:18155409-18155428 None:intergenic 30.0%
CTATCTCTGATGAATATTTG+AGG - chr2_1:18155460-18155479 Msa0199240:exon 30.0%
!! TTTGTGTTGTTGTTGATGAT+AGG - chr2_1:18155563-18155582 Msa0199240:intron 30.0%
CAACATTGATATCCTTGTTA+GGG + chr2_1:18155663-18155682 None:intergenic 30.0%
GACAATTGGAATGTTATAGA+AGG + chr2_1:18155686-18155705 None:intergenic 30.0%
ATAACATTCCAATTGTCAAG+AGG - chr2_1:18155689-18155708 Msa0199240:intron 30.0%
!! TGTAGTTGTGATTTAGTTTG+TGG - chr2_1:18155791-18155810 Msa0199240:CDS 30.0%
!! GTAGTTGTGATTTAGTTTGT+GGG - chr2_1:18155792-18155811 Msa0199240:CDS 30.0%
! GAAAATTTACTGATTGGCAA+AGG - chr2_1:18156203-18156222 Msa0199240:CDS 30.0%
AATGAAGAACGAGAAGATGA+AGG + chr2_1:18155253-18155272 None:intergenic 35.0%
GAATGAACTCTATTACGAAG+CGG - chr2_1:18155350-18155369 Msa0199240:intron 35.0%
TTCTGATGATAACCCTAACA+AGG - chr2_1:18155648-18155667 Msa0199240:intron 35.0%
GCAACATTGATATCCTTGTT+AGG + chr2_1:18155664-18155683 None:intergenic 35.0%
!! TGATTTAGTTTGTGGGTGTA+AGG - chr2_1:18155799-18155818 Msa0199240:intron 35.0%
!! GATTTAGTTTGTGGGTGTAA+GGG - chr2_1:18155800-18155819 Msa0199240:intron 35.0%
TTCGATGTCTTGTAGGTTAA+GGG - chr2_1:18155848-18155867 Msa0199240:intron 35.0%
! ACTTTCAAGTGTCTAGAATG+TGG - chr2_1:18155960-18155979 Msa0199240:CDS 35.0%
ACAGCAATTCAAGTATACTG+AGG - chr2_1:18156004-18156023 Msa0199240:CDS 35.0%
GTAGCATTTGGACAAATTGT+TGG + chr2_1:18156147-18156166 None:intergenic 35.0%
CTGTTGTTACATGTAGCATT+TGG + chr2_1:18156159-18156178 None:intergenic 35.0%
! TAAATTTTCTCTCTTGGCGA+AGG + chr2_1:18156192-18156211 None:intergenic 35.0%
CAAATAACATCCAATGACCT+AGG + chr2_1:18156274-18156293 None:intergenic 35.0%
CTAGGTCATTGGATGTTATT+TGG - chr2_1:18156272-18156291 Msa0199240:five_prime_UTR 35.0%
AATGAAGAACGAGAAGATGA+AGG + chr2_1:18155253-18155272 None:intergenic 35.0%
GAATGAACTCTATTACGAAG+CGG - chr2_1:18155350-18155369 Msa0199240:intron 35.0%
TTCTGATGATAACCCTAACA+AGG - chr2_1:18155648-18155667 Msa0199240:intron 35.0%
GCAACATTGATATCCTTGTT+AGG + chr2_1:18155664-18155683 None:intergenic 35.0%
!! TGATTTAGTTTGTGGGTGTA+AGG - chr2_1:18155799-18155818 Msa0199240:intron 35.0%
!! GATTTAGTTTGTGGGTGTAA+GGG - chr2_1:18155800-18155819 Msa0199240:intron 35.0%
TTCGATGTCTTGTAGGTTAA+GGG - chr2_1:18155848-18155867 Msa0199240:intron 35.0%
! ACTTTCAAGTGTCTAGAATG+TGG - chr2_1:18155960-18155979 Msa0199240:CDS 35.0%
ACAGCAATTCAAGTATACTG+AGG - chr2_1:18156004-18156023 Msa0199240:CDS 35.0%
GTAGCATTTGGACAAATTGT+TGG + chr2_1:18156147-18156166 None:intergenic 35.0%
CTGTTGTTACATGTAGCATT+TGG + chr2_1:18156159-18156178 None:intergenic 35.0%
! TAAATTTTCTCTCTTGGCGA+AGG + chr2_1:18156192-18156211 None:intergenic 35.0%
CAAATAACATCCAATGACCT+AGG + chr2_1:18156274-18156293 None:intergenic 35.0%
CTAGGTCATTGGATGTTATT+TGG - chr2_1:18156272-18156291 Msa0199240:five_prime_UTR 35.0%
! TTCAACCGATTCAGCGAAAA+TGG - chr2_1:18155130-18155149 Msa0199240:three_prime_UTR 40.0%
! TGCTTCCATTTTCGCTGAAT+CGG + chr2_1:18155138-18155157 None:intergenic 40.0%
!!! GAAAGCGTTTTCAACCCTAA+CGG + chr2_1:18155189-18155208 None:intergenic 40.0%
! CCTTTGTCCAGATTTGAAAC+TGG + chr2_1:18155331-18155350 None:intergenic 40.0%
CCAGTTTCAAATCTGGACAA+AGG - chr2_1:18155328-18155347 Msa0199240:intron 40.0%
TGCATACACCTCTTGACAAT+TGG + chr2_1:18155700-18155719 None:intergenic 40.0%
GTATGTGTTCGATGTCTTGT+AGG - chr2_1:18155841-18155860 Msa0199240:intron 40.0%
GTTCGATGTCTTGTAGGTTA+AGG - chr2_1:18155847-18155866 Msa0199240:intron 40.0%
GTCTTGTAGGTTAAGGGAAT+TGG - chr2_1:18155854-18155873 Msa0199240:intron 40.0%
GAATTGGCTACATCAGAGAT+TGG - chr2_1:18155870-18155889 Msa0199240:intron 40.0%
CTAGACACTTGAAAGTTCCT+TGG + chr2_1:18155956-18155975 None:intergenic 40.0%
! TTCAAGTGTCTAGAATGTGG+TGG - chr2_1:18155963-18155982 Msa0199240:CDS 40.0%
! TCAAGTGTCTAGAATGTGGT+GGG - chr2_1:18155964-18155983 Msa0199240:CDS 40.0%
! TTCAACCGATTCAGCGAAAA+TGG - chr2_1:18155130-18155149 Msa0199240:three_prime_UTR 40.0%
! TGCTTCCATTTTCGCTGAAT+CGG + chr2_1:18155138-18155157 None:intergenic 40.0%
!!! GAAAGCGTTTTCAACCCTAA+CGG + chr2_1:18155189-18155208 None:intergenic 40.0%
! CCTTTGTCCAGATTTGAAAC+TGG + chr2_1:18155331-18155350 None:intergenic 40.0%
CCAGTTTCAAATCTGGACAA+AGG - chr2_1:18155328-18155347 Msa0199240:intron 40.0%
TGCATACACCTCTTGACAAT+TGG + chr2_1:18155700-18155719 None:intergenic 40.0%
GTATGTGTTCGATGTCTTGT+AGG - chr2_1:18155841-18155860 Msa0199240:intron 40.0%
GTTCGATGTCTTGTAGGTTA+AGG - chr2_1:18155847-18155866 Msa0199240:intron 40.0%
GTCTTGTAGGTTAAGGGAAT+TGG - chr2_1:18155854-18155873 Msa0199240:intron 40.0%
GAATTGGCTACATCAGAGAT+TGG - chr2_1:18155870-18155889 Msa0199240:intron 40.0%
CTAGACACTTGAAAGTTCCT+TGG + chr2_1:18155956-18155975 None:intergenic 40.0%
! TTCAAGTGTCTAGAATGTGG+TGG - chr2_1:18155963-18155982 Msa0199240:CDS 40.0%
! TCAAGTGTCTAGAATGTGGT+GGG - chr2_1:18155964-18155983 Msa0199240:CDS 40.0%
!! AAGCTTCTCTGAAACCTAGC+CGG + chr2_1:18155112-18155131 None:intergenic 45.0%
!! TCAGCGAAAATGGAAGCATC+CGG - chr2_1:18155140-18155159 Msa0199240:three_prime_UTR 45.0%
CTCATCAACCAAATAACCGC+CGG + chr2_1:18155162-18155181 None:intergenic 45.0%
!! TGGTTGATGAGAAAGCCGTT+AGG - chr2_1:18155171-18155190 Msa0199240:three_prime_UTR 45.0%
!! GGTTGATGAGAAAGCCGTTA+GGG - chr2_1:18155172-18155191 Msa0199240:three_prime_UTR 45.0%
TGGATCGCCAGTTTCAAATC+TGG - chr2_1:18155321-18155340 Msa0199240:intron 45.0%
TTGCAGGCATTCTGCAAGTA+AGG + chr2_1:18155595-18155614 None:intergenic 45.0%
!! TGCCTGCAAGAGGTTTGTTA+TGG - chr2_1:18155606-18155625 Msa0199240:intron 45.0%
CTCCATAACAAACCTCTTGC+AGG + chr2_1:18155611-18155630 None:intergenic 45.0%
TGGCTACATCAGAGATTGGT+AGG - chr2_1:18155874-18155893 Msa0199240:intron 45.0%
GGTAGGCTTGTATCTGTAAC+AGG - chr2_1:18155891-18155910 Msa0199240:intron 45.0%
GCTTGTATCTGTAACAGGAG+TGG - chr2_1:18155896-18155915 Msa0199240:intron 45.0%
CTGTAACAGGAGTGGTAACA+AGG - chr2_1:18155904-18155923 Msa0199240:intron 45.0%
AGTGGTAACAAGGACAAGTG+AGG - chr2_1:18155914-18155933 Msa0199240:intron 45.0%
AAAGTTCCTTGGAGAAGCTC+AGG + chr2_1:18155945-18155964 None:intergenic 45.0%
! CAAGTGTCTAGAATGTGGTG+GGG - chr2_1:18155965-18155984 Msa0199240:CDS 45.0%
ACGTCCAATGAGATACCTGT+TGG - chr2_1:18156241-18156260 Msa0199240:exon 45.0%
AGAGCCAACAGGTATCTCAT+TGG + chr2_1:18156248-18156267 None:intergenic 45.0%
!! AAGCTTCTCTGAAACCTAGC+CGG + chr2_1:18155112-18155131 None:intergenic 45.0%
!! TCAGCGAAAATGGAAGCATC+CGG - chr2_1:18155140-18155159 Msa0199240:three_prime_UTR 45.0%
CTCATCAACCAAATAACCGC+CGG + chr2_1:18155162-18155181 None:intergenic 45.0%
!! TGGTTGATGAGAAAGCCGTT+AGG - chr2_1:18155171-18155190 Msa0199240:three_prime_UTR 45.0%
!! GGTTGATGAGAAAGCCGTTA+GGG - chr2_1:18155172-18155191 Msa0199240:three_prime_UTR 45.0%
TGGATCGCCAGTTTCAAATC+TGG - chr2_1:18155321-18155340 Msa0199240:intron 45.0%
TTGCAGGCATTCTGCAAGTA+AGG + chr2_1:18155595-18155614 None:intergenic 45.0%
!! TGCCTGCAAGAGGTTTGTTA+TGG - chr2_1:18155606-18155625 Msa0199240:intron 45.0%
CTCCATAACAAACCTCTTGC+AGG + chr2_1:18155611-18155630 None:intergenic 45.0%
TGGCTACATCAGAGATTGGT+AGG - chr2_1:18155874-18155893 Msa0199240:intron 45.0%
GGTAGGCTTGTATCTGTAAC+AGG - chr2_1:18155891-18155910 Msa0199240:intron 45.0%
GCTTGTATCTGTAACAGGAG+TGG - chr2_1:18155896-18155915 Msa0199240:intron 45.0%
CTGTAACAGGAGTGGTAACA+AGG - chr2_1:18155904-18155923 Msa0199240:intron 45.0%
AGTGGTAACAAGGACAAGTG+AGG - chr2_1:18155914-18155933 Msa0199240:intron 45.0%
AAAGTTCCTTGGAGAAGCTC+AGG + chr2_1:18155945-18155964 None:intergenic 45.0%
! CAAGTGTCTAGAATGTGGTG+GGG - chr2_1:18155965-18155984 Msa0199240:CDS 45.0%
ACGTCCAATGAGATACCTGT+TGG - chr2_1:18156241-18156260 Msa0199240:exon 45.0%
AGAGCCAACAGGTATCTCAT+TGG + chr2_1:18156248-18156267 None:intergenic 45.0%
ACTTGCAGAATGCCTGCAAG+AGG - chr2_1:18155596-18155615 Msa0199240:intron 50.0%
GTTTGACCTGAGCTTCTCCA+AGG - chr2_1:18155936-18155955 Msa0199240:CDS 50.0%
TTGGCAAAGGGTCAGAATGC+AGG - chr2_1:18156216-18156235 Msa0199240:CDS 50.0%
ACTTGCAGAATGCCTGCAAG+AGG - chr2_1:18155596-18155615 Msa0199240:intron 50.0%
GTTTGACCTGAGCTTCTCCA+AGG - chr2_1:18155936-18155955 Msa0199240:CDS 50.0%
TTGGCAAAGGGTCAGAATGC+AGG - chr2_1:18156216-18156235 Msa0199240:CDS 50.0%
GGAAAACGACGTCGGAGCAA+GGG + chr2_1:18155091-18155110 None:intergenic 55.0%
!! CTTGCTCCGACGTCGTTTTC+CGG - chr2_1:18155090-18155109 Msa0199240:three_prime_UTR 55.0%
ACCTAGCCGGAAAACGACGT+CGG + chr2_1:18155099-18155118 None:intergenic 55.0%
!! GCGAAAATGGAAGCATCCGG+CGG - chr2_1:18155143-18155162 Msa0199240:three_prime_UTR 55.0%
!! GGAAGCATCCGGCGGTTATT+TGG - chr2_1:18155151-18155170 Msa0199240:three_prime_UTR 55.0%
!! TACCTGTTGGCTCTCTGCCT+AGG - chr2_1:18156254-18156273 Msa0199240:five_prime_UTR 55.0%
TGGCTCTCTGCCTAGGTCAT+TGG - chr2_1:18156261-18156280 Msa0199240:five_prime_UTR 55.0%
GGAAAACGACGTCGGAGCAA+GGG + chr2_1:18155091-18155110 None:intergenic 55.0%
!! CTTGCTCCGACGTCGTTTTC+CGG - chr2_1:18155090-18155109 Msa0199240:three_prime_UTR 55.0%
ACCTAGCCGGAAAACGACGT+CGG + chr2_1:18155099-18155118 None:intergenic 55.0%
!! GCGAAAATGGAAGCATCCGG+CGG - chr2_1:18155143-18155162 Msa0199240:three_prime_UTR 55.0%
!! GGAAGCATCCGGCGGTTATT+TGG - chr2_1:18155151-18155170 Msa0199240:three_prime_UTR 55.0%
!! TACCTGTTGGCTCTCTGCCT+AGG - chr2_1:18156254-18156273 Msa0199240:five_prime_UTR 55.0%
TGGCTCTCTGCCTAGGTCAT+TGG - chr2_1:18156261-18156280 Msa0199240:five_prime_UTR 55.0%
CGGAAAACGACGTCGGAGCA+AGG + chr2_1:18155092-18155111 None:intergenic 60.0%
!! TCCGACGTCGTTTTCCGGCT+AGG - chr2_1:18155095-18155114 Msa0199240:three_prime_UTR 60.0%
GACCTAGGCAGAGAGCCAAC+AGG + chr2_1:18156259-18156278 None:intergenic 60.0%
CGGAAAACGACGTCGGAGCA+AGG + chr2_1:18155092-18155111 None:intergenic 60.0%
!! TCCGACGTCGTTTTCCGGCT+AGG - chr2_1:18155095-18155114 Msa0199240:three_prime_UTR 60.0%
GACCTAGGCAGAGAGCCAAC+AGG + chr2_1:18156259-18156278 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 18155087 18156315 18155087 ID=Msa0199240;Name=Msa0199240
chr2_1 mRNA 18155087 18156315 18155087 ID=Msa0199240-mRNA-1;Parent=Msa0199240;Name=Msa0199240-mRNA-1;_AED=0.00;_eAED=0.00;_QI=62|1|1|1|1|0.6|5|256|143
chr2_1 exon 18155087 18155258 18155087 ID=Msa0199240-mRNA-1:exon:7693;Parent=Msa0199240-mRNA-1
chr2_1 exon 18155377 18155539 18155377 ID=Msa0199240-mRNA-1:exon:7692;Parent=Msa0199240-mRNA-1
chr2_1 exon 18155692 18155817 18155692 ID=Msa0199240-mRNA-1:exon:7691;Parent=Msa0199240-mRNA-1
chr2_1 exon 18155921 18156070 18155921 ID=Msa0199240-mRNA-1:exon:7690;Parent=Msa0199240-mRNA-1
chr2_1 exon 18156177 18156315 18156177 ID=Msa0199240-mRNA-1:exon:7689;Parent=Msa0199240-mRNA-1
chr2_1 five_prime_UTR 18156254 18156315 18156254 ID=Msa0199240-mRNA-1:five_prime_utr;Parent=Msa0199240-mRNA-1
chr2_1 CDS 18156177 18156253 18156177 ID=Msa0199240-mRNA-1:cds;Parent=Msa0199240-mRNA-1
chr2_1 CDS 18155921 18156070 18155921 ID=Msa0199240-mRNA-1:cds;Parent=Msa0199240-mRNA-1
chr2_1 CDS 18155692 18155817 18155692 ID=Msa0199240-mRNA-1:cds;Parent=Msa0199240-mRNA-1
chr2_1 CDS 18155461 18155539 18155461 ID=Msa0199240-mRNA-1:cds;Parent=Msa0199240-mRNA-1
chr2_1 three_prime_UTR 18155377 18155460 18155377 ID=Msa0199240-mRNA-1:three_prime_utr;Parent=Msa0199240-mRNA-1
chr2_1 three_prime_UTR 18155087 18155258 18155087 ID=Msa0199240-mRNA-1:three_prime_utr;Parent=Msa0199240-mRNA-1
Gene Sequence

>Msa0199240

ATGGAAGCATCCGGCGGTTATTTGGTTGATGAGAAAGCCGTTAGGGTTGAAAACGCTTTCTTAGATTTTCTCAAAAGTTTCAAATCTGGACAAAGGAATGAACTCTATTACGAAGCGGAGATTGAAGTCATGAGAGCTAACGAATCCAACACCATGTTCATTGATTTTGAACGTGTGATTAGATTCAGTGATGTTCTTCAGAAAGCTATCTCTGATGAATATTTGAGGTTTGAACCTTACTTGCAGAATGCCTGCAAGAGGTTTGTTATGGAGCTTAAGCCGACATTCATTTCTGATGATAACCCTAACAAGGATATCAATGTTGCCTTCTATAACATTCCAATTGTCAAGAGGTTAAGGGAATTGGCTACATCAGAGATTGGTAGGCTTGTATCTGTAACAGGAGTGGTAACAAGGACAAGTGAGGTTTGA
Protein sequence

>Msa0199240

MEASGGYLVDEKAVRVENAFLDFLKSFKSGQRNELYYEAEIEVMRANESNTMFIDFERVIRFSDVLQKAISDEYLRFEPYLQNACKRFVMELKPTFISDDNPNKDINVAFYNIPIVKRLRELATSEIGRLVSVTGVVTRTSEV*