AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0824600


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 20 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCGTTTGTTAAATCATTT+AGG 0.281266 5_3:+64026515 Msa0824600:CDS
CGAGCTGCTAGATTAGGTTT+TGG 0.331839 5_3:+64026424 Msa0824600:CDS
GCTGTTTGGGAACTAGTTAA+TGG 0.356582 5_3:+64025864 Msa0824600:CDS
CAAGATTGAGATGGCTGTTT+GGG 0.360205 5_3:+64025851 None:intergenic
CCACAGTGTTTACTAATAGA+TGG 0.393695 5_3:+64025952 Msa0824600:CDS
TCCTAGCTAGAAAGGTGATA+TGG 0.410532 5_3:-64025896 None:intergenic
TCACTGCGAGCTGCTAGATT+AGG 0.430552 5_3:+64026418 Msa0824600:CDS
TCAAGATTGAGATGGCTGTT+TGG 0.443129 5_3:+64025850 None:intergenic
CGGATAAGATGGAAGATAAG+AGG 0.483166 5_3:-64026464 None:intergenic
TAGCATAATAACGGATAAGA+TGG 0.485732 5_3:-64026475 None:intergenic
ACCATATCACCTTTCTAGCT+AGG 0.492797 5_3:+64025895 Msa0824600:CDS
TCTTATCCGTTATTATGCTA+TGG 0.498677 5_3:+64026478 Msa0824600:CDS
CTAGAAAGGTGATATGGTAA+AGG 0.506004 5_3:-64025890 None:intergenic
ATCAGCATTCCTAGCTAGAA+AGG 0.535370 5_3:-64025904 None:intergenic
AGCTTGCCATAGCATAATAA+CGG 0.565019 5_3:-64026484 None:intergenic
AAGATGGAAGATAAGAGGAT+CGG 0.605242 5_3:-64026459 None:intergenic
TGTTTACTAATAGATGGATG+AGG 0.629998 5_3:+64025958 Msa0824600:CDS
TACTAATAGATGGATGAGGA+AGG 0.630665 5_3:+64025962 Msa0824600:CDS
ATGATTTAACAAACGAACGC+AGG 0.657550 5_3:-64026510 None:intergenic
CCATCTATTAGTAAACACTG+TGG 0.792095 5_3:-64025952 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGCAAATAATATTTTTGTTA+GGG - chr5_3:64026229-64026248 None:intergenic 15.0%
!!! AAGCAAATAATATTTTTGTT+AGG - chr5_3:64026230-64026249 None:intergenic 15.0%
!!! GCAAATAATATTTTTGTTAG+GGG - chr5_3:64026228-64026247 None:intergenic 20.0%
! ATAGACTGAATTTACATAAC+TGG - chr5_3:64026279-64026298 None:intergenic 25.0%
! TGTACTCTAAATCATATAGA+AGG - chr5_3:64026328-64026347 None:intergenic 25.0%
!! GGTTCTTGTTTACATAATTA+CGG + chr5_3:64026357-64026376 Msa0824600:intron 25.0%
!! GTTCTTGTTTACATAATTAC+GGG + chr5_3:64026358-64026377 Msa0824600:intron 25.0%
!! GTTCGTTTGTTAAATCATTT+AGG + chr5_3:64026515-64026534 Msa0824600:CDS 25.0%
TGTTTACTAATAGATGGATG+AGG + chr5_3:64025958-64025977 Msa0824600:CDS 30.0%
GCAAAATAAGAAAGACTGAA+AGG - chr5_3:64026053-64026072 None:intergenic 30.0%
!!! AGTTTTTTGTCCAACGAAAA+AGG + chr5_3:64026162-64026181 Msa0824600:intron 30.0%
!!! GTTTTTTGTCCAACGAAAAA+GGG + chr5_3:64026163-64026182 Msa0824600:intron 30.0%
!!! TTTTTTGTCCAACGAAAAAG+GGG + chr5_3:64026164-64026183 Msa0824600:intron 30.0%
ACTGAATTTACATAACTGGA+AGG - chr5_3:64026275-64026294 None:intergenic 30.0%
! ATTTAGAGTACATGCTCATT+TGG + chr5_3:64026336-64026355 Msa0824600:intron 30.0%
!!! TTTGTTTGCTTGTTTTGTTC+AGG + chr5_3:64026384-64026403 Msa0824600:intron 30.0%
!! TAGCATAATAACGGATAAGA+TGG - chr5_3:64026478-64026497 None:intergenic 30.0%
TCTTATCCGTTATTATGCTA+TGG + chr5_3:64026478-64026497 Msa0824600:CDS 30.0%
CTAGAAAGGTGATATGGTAA+AGG - chr5_3:64025893-64025912 None:intergenic 35.0%
CCATCTATTAGTAAACACTG+TGG - chr5_3:64025955-64025974 None:intergenic 35.0%
CCACAGTGTTTACTAATAGA+TGG + chr5_3:64025952-64025971 Msa0824600:CDS 35.0%
TACTAATAGATGGATGAGGA+AGG + chr5_3:64025962-64025981 Msa0824600:CDS 35.0%
GAATCAAAAGTAGCGATGTT+TGG + chr5_3:64025997-64026016 Msa0824600:intron 35.0%
TCAGATGCAGAGAAACATAT+TGG - chr5_3:64026117-64026136 None:intergenic 35.0%
AAGATGGAAGATAAGAGGAT+CGG - chr5_3:64026462-64026481 None:intergenic 35.0%
AGCTTGCCATAGCATAATAA+CGG - chr5_3:64026487-64026506 None:intergenic 35.0%
ATGATTTAACAAACGAACGC+AGG - chr5_3:64026513-64026532 None:intergenic 35.0%
GCTGTTTGGGAACTAGTTAA+TGG + chr5_3:64025864-64025883 Msa0824600:CDS 40.0%
!! ACCATATCACCTTTCTAGCT+AGG + chr5_3:64025895-64025914 Msa0824600:CDS 40.0%
TCCTAGCTAGAAAGGTGATA+TGG - chr5_3:64025899-64025918 None:intergenic 40.0%
ATCAGCATTCCTAGCTAGAA+AGG - chr5_3:64025907-64025926 None:intergenic 40.0%
ATAGATGGATGAGGAAGGTT+CGG + chr5_3:64025967-64025986 Msa0824600:intron 40.0%
GCTCTCAAGAATCAAGGTAA+AGG - chr5_3:64026306-64026325 None:intergenic 40.0%
TAGAAGGCTCTCAAGAATCA+AGG - chr5_3:64026312-64026331 None:intergenic 40.0%
!!! CTTGTTTTGTTCAGGTACGT+TGG + chr5_3:64026392-64026411 Msa0824600:intron 40.0%
CGGATAAGATGGAAGATAAG+AGG - chr5_3:64026467-64026486 None:intergenic 40.0%
GTCCAACGAAAAAGGGGATA+AGG + chr5_3:64026170-64026189 Msa0824600:intron 45.0%
!! AGCCTTATCCCCTTTTTCGT+TGG - chr5_3:64026175-64026194 None:intergenic 45.0%
! CGAGCTGCTAGATTAGGTTT+TGG + chr5_3:64026424-64026443 Msa0824600:CDS 45.0%
ATGAGGAAGGTTCGGAGAGT+CGG + chr5_3:64025975-64025994 Msa0824600:intron 50.0%
TCACTGCGAGCTGCTAGATT+AGG + chr5_3:64026418-64026437 Msa0824600:CDS 50.0%
AAGGCTAACCTCCCATGCAG+TGG + chr5_3:64026189-64026208 Msa0824600:intron 55.0%
AGGCTAACCTCCCATGCAGT+GGG + chr5_3:64026190-64026209 Msa0824600:intron 55.0%
AGCTAACACCCCCACTGCAT+GGG - chr5_3:64026203-64026222 None:intergenic 55.0%
TAGCTAACACCCCCACTGCA+TGG - chr5_3:64026204-64026223 None:intergenic 55.0%
GGCTAACCTCCCATGCAGTG+GGG + chr5_3:64026191-64026210 Msa0824600:intron 60.0%
GCTAACCTCCCATGCAGTGG+GGG + chr5_3:64026192-64026211 Msa0824600:intron 60.0%
TAACACCCCCACTGCATGGG+AGG - chr5_3:64026200-64026219 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 64025861 64026549 64025861 ID=Msa0824600;Name=Msa0824600
chr5_3 mRNA 64025861 64026549 64025861 ID=Msa0824600-mRNA-1;Parent=Msa0824600;Name=Msa0824600-mRNA-1;_AED=0.64;_eAED=0.65;_QI=0|0|0|0.5|1|1|2|0|88
chr5_3 exon 64025861 64025983 64025861 ID=Msa0824600-mRNA-1:exon:19974;Parent=Msa0824600-mRNA-1
chr5_3 exon 64026406 64026549 64026406 ID=Msa0824600-mRNA-1:exon:19975;Parent=Msa0824600-mRNA-1
chr5_3 CDS 64025861 64025983 64025861 ID=Msa0824600-mRNA-1:cds;Parent=Msa0824600-mRNA-1
chr5_3 CDS 64026406 64026549 64026406 ID=Msa0824600-mRNA-1:cds;Parent=Msa0824600-mRNA-1
Gene Sequence

>Msa0824600

ATGGCTGTTTGGGAACTAGTTAATGGAGTCCTTTACCATATCACCTTTCTAGCTAGGAATGCTGATGAGAAGAAAAATGAAACTTTTGAAGCCACAGTGTTTACTAATAGATGGATGAGGAAGGTACGTTGGCACTCACTGCGAGCTGCTAGATTAGGTTTTGGATCTCATGCAATCCGATCCTCTTATCTTCCATCTTATCCGTTATTATGCTATGGCAAGCTGCACCTGCGTTCGTTTGTTAAATCATTTAGGAAGTTCTGCTAG

Protein sequence

>Msa0824600

MAVWELVNGVLYHITFLARNADEKKNETFEATVFTNRWMRKVRWHSLRAARLGFGSHAIRSSYLPSYPLLCYGKLHLRSFVKSFRKFC*