Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0021980 | Msa0265630 | 0.817840 | 2.676022e-52 | -8.615850e-47 |
| Msa0047230 | Msa0265630 | 0.895520 | 8.863082e-76 | -8.615850e-47 |
| Msa0053960 | Msa0265630 | 0.808074 | 3.703592e-50 | -8.615850e-47 |
| Msa0130100 | Msa0265630 | 0.809089 | 2.248097e-50 | -8.615850e-47 |
| Msa0153440 | Msa0265630 | 0.842298 | 2.817270e-58 | -8.615850e-47 |
| Msa0181410 | Msa0265630 | 0.815625 | 8.398166e-52 | -8.615850e-47 |
| Msa0208390 | Msa0265630 | 0.801525 | 8.640497e-49 | -8.615850e-47 |
| Msa0264940 | Msa0265630 | -0.829927 | 3.914612e-55 | -8.615850e-47 |
| Msa0265620 | Msa0265630 | 0.839864 | 1.227484e-57 | -8.615850e-47 |
| Msa0265630 | Msa0272420 | 0.817143 | 3.842601e-52 | -8.615850e-47 |
| Msa0265630 | Msa0302990 | -0.831713 | 1.428196e-55 | -8.615850e-47 |
| Msa0265630 | Msa0331590 | 0.800776 | 1.229704e-48 | -8.615850e-47 |
| Msa0265630 | Msa0340530 | 0.912807 | 1.274653e-83 | -8.615850e-47 |
| Msa0265630 | Msa0389050 | 0.823537 | 1.313178e-53 | -8.615850e-47 |
| Msa0265630 | Msa0457370 | 0.804956 | 1.683762e-49 | -8.615850e-47 |
| Msa0265630 | Msa0481320 | 0.800261 | 1.566138e-48 | -8.615850e-47 |
| Msa0265630 | Msa0535020 | 0.805101 | 1.570360e-49 | -8.615850e-47 |
| Msa0265630 | Msa0535050 | 0.801170 | 1.021767e-48 | -8.615850e-47 |
| Msa0265630 | Msa0563140 | 0.801986 | 6.951542e-49 | -8.615850e-47 |
| Msa0265630 | Msa0589610 | 0.805049 | 1.610380e-49 | -8.615850e-47 |
| Msa0265630 | Msa0591780 | 0.801088 | 1.062033e-48 | -8.615850e-47 |
| Msa0265630 | Msa0599250 | 0.818393 | 2.006379e-52 | -8.615850e-47 |
| Msa0265630 | Msa0613480 | 0.801016 | 1.098540e-48 | -8.615850e-47 |
| Msa0265630 | Msa0641020 | 0.801956 | 7.050553e-49 | -8.615850e-47 |
| Msa0265630 | Msa0664020 | 0.806308 | 8.763087e-50 | -8.615850e-47 |
| Msa0265630 | Msa0672850 | 0.811235 | 7.749359e-51 | -8.615850e-47 |
| Msa0265630 | Msa0678240 | 0.807216 | 5.633079e-50 | -8.615850e-47 |
| Msa0265630 | Msa0715310 | 0.823108 | 1.654492e-53 | -8.615850e-47 |
| Msa0265630 | Msa0722930 | 0.826656 | 2.407298e-54 | -8.615850e-47 |
| Msa0265630 | Msa0723140 | 0.839567 | 1.466404e-57 | -8.615850e-47 |
| Msa0265630 | Msa0744500 | 0.822976 | 1.775613e-53 | -8.615850e-47 |
| Msa0265630 | Msa0748610 | 0.804523 | 2.073367e-49 | -8.615850e-47 |
| Msa0265630 | Msa0752580 | 0.805279 | 1.441211e-49 | -8.615850e-47 |
| Msa0265630 | Msa0754500 | 0.804983 | 1.662128e-49 | -8.615850e-47 |
| Msa0265630 | Msa0788360 | 0.824485 | 7.867844e-54 | -8.615850e-47 |
| Msa0265630 | Msa0792880 | 0.804038 | 2.616256e-49 | -8.615850e-47 |
| Msa0265630 | Msa0806210 | 0.802371 | 5.792083e-49 | -8.615850e-47 |
| Msa0265630 | Msa0826650 | 0.832983 | 6.924574e-56 | -8.615850e-47 |
| Msa0265630 | Msa0828570 | 0.801444 | 8.978399e-49 | -8.615850e-47 |
| Msa0265630 | Msa0828900 | 0.807680 | 4.491240e-50 | -8.615850e-47 |
| Msa0265630 | Msa0836680 | 0.805652 | 1.203689e-49 | -8.615850e-47 |
| Msa0265630 | Msa0842050 | 0.803825 | 2.896897e-49 | -8.615850e-47 |
| Msa0265630 | Msa0843280 | 0.817463 | 3.254846e-52 | -8.615850e-47 |
| Msa0265630 | Msa0864690 | 0.833248 | 5.948128e-56 | -8.615850e-47 |
| Msa0265630 | Msa0883660 | 0.828470 | 8.830855e-55 | -8.615850e-47 |
| Msa0265630 | Msa0883670 | 0.801968 | 7.007857e-49 | -8.615850e-47 |
| Msa0265630 | Msa0887000 | 0.809467 | 1.865148e-50 | -8.615850e-47 |
| Msa0265630 | Msa0902980 | 0.825886 | 3.671221e-54 | -8.615850e-47 |
| Msa0265630 | Msa0951720 | 0.809495 | 1.839990e-50 | -8.615850e-47 |
| Msa0265630 | Msa0988500 | 0.802691 | 4.975451e-49 | -8.615850e-47 |
| Msa0265630 | Msa1008340 | 0.828380 | 9.287038e-55 | -8.615850e-47 |
| Msa0265630 | Msa1049950 | 0.833987 | 3.888258e-56 | -8.615850e-47 |
| Msa0265630 | Msa1113210 | 0.821491 | 3.925738e-53 | -8.615850e-47 |
| Msa0265630 | Msa1113280 | 0.812599 | 3.909990e-51 | -8.615850e-47 |
| Msa0265630 | Msa1138450 | 0.823716 | 1.192218e-53 | -8.615850e-47 |
| Msa0265630 | Msa1138480 | 0.812137 | 4.931142e-51 | -8.615850e-47 |
| Msa0265630 | Msa1139240 | 0.819188 | 1.324388e-52 | -8.615850e-47 |
| Msa0265630 | Msa1182830 | 0.828730 | 7.642239e-55 | -8.615850e-47 |
| Msa0265630 | Msa1221200 | -0.804406 | 2.194099e-49 | -8.615850e-47 |
| Msa0265630 | Msa1237260 | 0.802988 | 4.319713e-49 | -8.615850e-47 |
| Msa0265630 | Msa1258280 | 0.832929 | 7.141622e-56 | -8.615850e-47 |
| Msa0265630 | Msa1298290 | 0.824521 | 7.715415e-54 | -8.615850e-47 |
| Msa0265630 | Msa1304850 | 0.824718 | 6.933226e-54 | -8.615850e-47 |
| Msa0265630 | Msa1311150 | 0.801886 | 7.286936e-49 | -8.615850e-47 |
| Msa0265630 | Msa1328600 | 0.823353 | 1.450001e-53 | -8.615850e-47 |
| Msa0265630 | Msa1340530 | 0.829404 | 5.245986e-55 | -8.615850e-47 |
| Msa0265630 | Msa1386020 | 0.815629 | 8.383115e-52 | -8.615850e-47 |
| Msa0265630 | Msa1457070 | 0.803637 | 3.170542e-49 | -8.615850e-47 |
| Msa0265630 | Msa1460310 | -0.831648 | 1.481947e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0265630 | MtrunA17_Chr8g0347741 | 57.895 | 57 | 3 | 1 | 15 | 50 | 345 | 401 | 7.61e-11 | 55.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCCAACGGCATTATTAT+AGG | 0.241535 | 2_2:+65971035 | Msa0265630:CDS |
| AACCCAACGGCATTATTATA+GGG | 0.336990 | 2_2:+65971036 | Msa0265630:CDS |
| AGGGCTACAGAGGAAGAAAA+TGG | 0.427836 | 2_2:+65970999 | Msa0265630:CDS |
| GAAGCTGGAAAACACTCAAT+TGG | 0.435156 | 2_2:+65970938 | Msa0265630:CDS |
| AACCCTATAATAATGCCGTT+GGG | 0.507995 | 2_2:-65971038 | None:intergenic |
| CAAAGCATGGACACAGACAC+TGG | 0.509651 | 2_2:+65970735 | None:intergenic |
| TAACCCTATAATAATGCCGT+TGG | 0.527045 | 2_2:-65971039 | None:intergenic |
| TGTGAACGGCATTGACAAGA+GGG | 0.593449 | 2_2:+65970980 | Msa0265630:CDS |
| CGACATTGACACATCATCAC+CGG | 0.607876 | 2_2:+65970761 | Msa0265630:CDS |
| TTGTGAACGGCATTGACAAG+AGG | 0.625018 | 2_2:+65970979 | Msa0265630:CDS |
| CATTGACAAGAGGGCTACAG+AGG | 0.633228 | 2_2:+65970989 | Msa0265630:CDS |
| AAAGCATGGACACAGACACT+GGG | 0.645560 | 2_2:+65970736 | None:intergenic |
| TTCTGTGAGAACAAACCCAA+CGG | 0.657855 | 2_2:+65971023 | Msa0265630:CDS |
| ACAGAAGAGATAATTGTGAA+CGG | 0.661430 | 2_2:+65970966 | Msa0265630:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAGTCAAAATTATCTATAAT+TGG | - | chr2_2:65970914-65970933 | None:intergenic | 20.0% |
| !!! | ATTATAGATAATTTTGACTC+AGG | + | chr2_2:65970914-65970933 | Msa0265630:intron | 20.0% |
| ! | CTATTTAGTCAAATTATCAC+CGG | - | chr2_2:65970783-65970802 | None:intergenic | 25.0% |
| ACAGAAGAGATAATTGTGAA+CGG | + | chr2_2:65970966-65970985 | Msa0265630:CDS | 30.0% | |
| AAACCCAACGGCATTATTAT+AGG | + | chr2_2:65971035-65971054 | Msa0265630:CDS | 35.0% | |
| AACCCAACGGCATTATTATA+GGG | + | chr2_2:65971036-65971055 | Msa0265630:CDS | 35.0% | |
| AACCCTATAATAATGCCGTT+GGG | - | chr2_2:65971041-65971060 | None:intergenic | 35.0% | |
| TAACCCTATAATAATGCCGT+TGG | - | chr2_2:65971042-65971061 | None:intergenic | 35.0% | |
| !!! | AATTTTGACTCAGGAGAAGC+TGG | + | chr2_2:65970923-65970942 | Msa0265630:intron | 40.0% |
| GAAGCTGGAAAACACTCAAT+TGG | + | chr2_2:65970938-65970957 | Msa0265630:CDS | 40.0% | |
| TTCTGTGAGAACAAACCCAA+CGG | + | chr2_2:65971023-65971042 | Msa0265630:CDS | 40.0% | |
| CGACATTGACACATCATCAC+CGG | + | chr2_2:65970761-65970780 | Msa0265630:CDS | 45.0% | |
| TACAAATGTCAATGCCGTGC+TGG | + | chr2_2:65970819-65970838 | Msa0265630:intron | 45.0% | |
| !! | TTGTGAACGGCATTGACAAG+AGG | + | chr2_2:65970979-65970998 | Msa0265630:CDS | 45.0% |
| !! | TGTGAACGGCATTGACAAGA+GGG | + | chr2_2:65970980-65970999 | Msa0265630:CDS | 45.0% |
| AGGGCTACAGAGGAAGAAAA+TGG | + | chr2_2:65970999-65971018 | Msa0265630:CDS | 45.0% | |
| TCATGTGTCTGACACCAGCA+CGG | - | chr2_2:65970836-65970855 | None:intergenic | 50.0% | |
| CATTGACAAGAGGGCTACAG+AGG | + | chr2_2:65970989-65971008 | Msa0265630:CDS | 50.0% | |
| GCAGCGACGCGTCATATTGA+AGG | - | chr2_2:65970866-65970885 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_2 | gene | 65970741 | 65971067 | 65970741 | ID=Msa0265630;Name=Msa0265630 |
| chr2_2 | mRNA | 65970741 | 65971067 | 65970741 | ID=Msa0265630-mRNA-1;Parent=Msa0265630;Name=Msa0265630-mRNA-1;_AED=0.47;_eAED=0.72;_QI=0|0|0|1|1|1|2|0|57 |
| chr2_2 | exon | 65970741 | 65970782 | 65970741 | ID=Msa0265630-mRNA-1:exon:20812;Parent=Msa0265630-mRNA-1 |
| chr2_2 | exon | 65970936 | 65971067 | 65970936 | ID=Msa0265630-mRNA-1:exon:20813;Parent=Msa0265630-mRNA-1 |
| chr2_2 | CDS | 65970741 | 65970782 | 65970741 | ID=Msa0265630-mRNA-1:cds;Parent=Msa0265630-mRNA-1 |
| chr2_2 | CDS | 65970936 | 65971067 | 65970936 | ID=Msa0265630-mRNA-1:cds;Parent=Msa0265630-mRNA-1 |
| Gene Sequence |
| Protein sequence |
