AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0130100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0130100 MtrunA17_Chr1g0197391 97.018 570 17 0 1 570 1 570 0.0 1133
Msa0130100 MtrunA17_Chr4g0028411 43.534 549 295 7 24 566 3 542 4.06e-151 445
Msa0130100 MtrunA17_Chr1g0157401 45.471 552 280 9 22 559 9 553 7.01e-151 445
Msa0130100 MtrunA17_Chr2g0327341 40.402 547 314 6 23 566 2 539 2.27e-143 425
Msa0130100 MtrunA17_Chr5g0409771 44.853 544 276 12 25 558 18 547 4.08e-141 420
Msa0130100 MtrunA17_Chr4g0039791 45.422 557 272 12 27 570 24 561 1.06e-140 419
Msa0130100 MtrunA17_Chr5g0409791 43.942 553 276 11 27 558 21 560 7.19e-137 409
Msa0130100 MtrunA17_Chr3g0116271 45.020 502 256 7 67 559 61 551 1.39e-133 400
Msa0130100 MtrunA17_Chr1g0180541 42.966 526 284 10 45 559 21 541 3.92e-131 394
Msa0130100 MtrunA17_Chr8g0353341 43.599 539 269 14 12 529 2 526 9.90e-128 385
Msa0130100 MtrunA17_Chr3g0116251 43.662 497 267 8 67 559 62 549 3.02e-126 382
Msa0130100 MtrunA17_Chr1g0180551 41.513 542 294 10 45 570 90 624 1.24e-123 377
Msa0130100 MtrunA17_Chr3g0102411 38.031 518 306 6 46 556 22 531 3.67e-114 350
Msa0130100 MtrunA17_Chr1g0186071 40.631 539 306 8 22 559 24 549 6.90e-114 350
Msa0130100 MtrunA17_Chr4g0072561 38.993 536 302 9 45 567 26 549 4.13e-109 337
Msa0130100 MtrunA17_Chr5g0395431 39.250 507 286 7 66 558 81 579 3.61e-102 320
Msa0130100 MtrunA17_Chr4g0000461 39.357 498 290 6 70 558 40 534 1.02e-100 315
Msa0130100 MtrunA17_Chr2g0334131 47.122 278 140 3 286 558 1 276 2.68e-79 251
Msa0130100 MtrunA17_Chr1g0157431 39.714 350 191 7 22 357 9 352 1.38e-64 220
Msa0130100 MtrunA17_Chr6g0471921 29.039 489 317 14 83 556 32 505 1.81e-37 145
Msa0130100 MtrunA17_Chr8g0370701 25.631 515 332 14 84 563 83 581 5.07e-36 142
Msa0130100 MtrunA17_Chr2g0301671 28.232 379 234 11 209 563 192 556 3.34e-34 136
Msa0130100 MtrunA17_Chr5g0433241 27.043 514 316 16 84 555 41 537 6.92e-34 135
Msa0130100 MtrunA17_Chr3g0109731 25.660 530 335 16 82 568 43 556 2.66e-33 134
Msa0130100 MtrunA17_Chr5g0396311 26.488 521 334 15 82 570 39 542 5.76e-33 132
Msa0130100 MtrunA17_Chr4g0068151 27.394 376 241 12 209 563 192 556 1.75e-30 125
Msa0130100 MtrunA17_Chr4g0028451 51.887 106 42 1 425 530 11 107 2.26e-29 112
Msa0130100 MtrunA17_Chr8g0370731 26.016 492 304 14 111 563 20 490 3.74e-29 121
Msa0130100 MtrunA17_Chr5g0396331 25.869 518 335 19 84 570 41 540 4.86e-29 121
Msa0130100 MtrunA17_Chr5g0433231 25.203 492 319 13 105 558 61 541 8.89e-28 117
Msa0130100 MtrunA17_Chr2g0292471 27.389 471 270 16 80 498 25 475 3.90e-26 112
Msa0130100 MtrunA17_Chr6g0471311 25.150 501 310 19 105 556 61 545 1.92e-24 107
Msa0130100 MtrunA17_Chr5g0433221 23.636 495 323 15 105 556 61 543 4.32e-22 100
Msa0130100 MtrunA17_Chr5g0406681 23.855 524 325 20 78 555 63 558 3.41e-20 94.4
Msa0130100 MtrunA17_Chr7g0252691 23.145 566 372 19 53 566 173 727 5.52e-19 90.9
Msa0130100 MtrunA17_Chr7g0270221 25.676 518 269 21 47 493 36 508 8.59e-18 87.0
Msa0130100 MtrunA17_Chr4g0004841 32.609 184 110 5 387 558 151 332 1.26e-17 84.3
Msa0130100 MtrunA17_Chr4g0004101 31.169 154 99 2 412 558 170 323 1.90e-17 84.0
Msa0130100 MtrunA17_Chr8g0351251 22.885 520 345 12 82 559 117 622 7.39e-14 75.1
Msa0130100 MtrunA17_Chr2g0334121 31.319 182 104 5 59 229 36 207 1.93e-13 69.7
Msa0130100 MtrunA17_Chr2g0329161 27.616 344 199 16 194 499 657 988 3.45e-13 73.2
Msa0130100 MtrunA17_Chr3g0121951 29.412 204 111 5 396 566 507 710 4.54e-13 72.0
Msa0130100 MtrunA17_Chr3g0121931 35.294 136 75 3 396 518 511 646 5.17e-12 68.9
Msa0130100 MtrunA17_Chr8g0377991 31.818 154 89 4 396 536 514 664 1.44e-11 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0130100 AT1G20510.1 45.438 548 282 8 18 559 1 537 3.77e-155 456
Msa0130100 AT1G20500.1 43.566 544 284 9 28 559 11 543 2.71e-146 433
Msa0130100 AT4G05160.1 42.650 551 299 6 24 566 3 544 1.25e-145 431
Msa0130100 AT4G19010.1 43.407 546 279 10 27 558 25 554 4.16e-139 415
Msa0130100 AT5G38120.1 42.463 544 289 9 28 559 12 543 5.15e-137 409
Msa0130100 AT1G20480.1 42.831 551 286 12 22 558 22 557 3.13e-135 405
Msa0130100 AT5G63380.1 42.287 551 292 11 22 558 12 550 2.46e-133 400
Msa0130100 AT1G20510.2 43.388 484 257 8 18 495 1 473 3.86e-122 369
Msa0130100 AT5G38120.3 40.971 515 280 9 28 530 12 514 1.65e-119 363
Msa0130100 AT1G20510.3 41.775 462 252 8 18 473 1 451 2.97e-109 335
Msa0130100 AT1G65060.1 41.309 489 276 5 78 558 71 556 1.06e-107 334
Msa0130100 AT5G38120.4 40.208 480 263 9 28 495 12 479 4.67e-106 327
Msa0130100 AT5G38120.2 40.208 480 263 9 28 495 12 479 4.67e-106 327
Msa0130100 AT3G21240.1 39.479 499 271 8 79 558 60 546 2.81e-105 328
Msa0130100 AT1G62940.1 40.927 496 280 6 71 558 42 532 6.89e-105 326
Msa0130100 AT3G21230.1 40.157 508 287 6 65 558 56 560 1.03e-102 322
Msa0130100 AT1G51680.1 39.087 504 289 7 70 558 53 553 8.15e-97 306
Msa0130100 AT3G21230.3 39.375 480 274 6 65 530 56 532 2.20e-90 289
Msa0130100 AT1G51680.3 38.235 476 276 7 70 530 53 525 2.39e-84 273
Msa0130100 AT1G20490.2 37.123 431 244 10 28 443 11 429 3.14e-79 256
Msa0130100 AT1G20490.1 37.123 431 244 10 28 443 11 429 3.14e-79 256
Msa0130100 AT3G21240.2 37.642 441 244 8 73 494 54 482 1.25e-78 256
Msa0130100 AT3G21240.3 37.642 441 244 8 73 494 54 482 1.25e-78 256
Msa0130100 AT3G21230.2 38.876 445 255 6 64 494 55 496 3.48e-78 255
Msa0130100 AT1G65060.2 38.670 406 238 5 78 475 71 473 2.16e-73 243
Msa0130100 AT1G51680.2 37.500 440 257 7 70 494 53 489 2.09e-71 237
Msa0130100 AT3G21230.4 34.584 373 227 6 65 423 56 425 4.64e-43 160
Msa0130100 AT3G48990.1 29.857 489 315 12 82 556 30 504 1.02e-40 155
Msa0130100 AT1G65880.1 27.920 548 323 17 82 569 32 567 2.11e-39 152
Msa0130100 AT1G30520.2 27.531 563 325 18 70 568 14 557 1.93e-38 149
Msa0130100 AT1G30520.1 27.531 563 325 18 70 568 14 557 1.93e-38 149
Msa0130100 AT1G65890.1 27.032 529 322 16 82 558 32 548 1.10e-36 144
Msa0130100 AT1G21540.1 27.606 518 319 17 79 555 37 539 5.03e-36 142
Msa0130100 AT1G20560.1 26.437 522 333 15 84 570 43 548 1.33e-33 135
Msa0130100 AT1G21530.2 25.769 520 325 17 78 555 38 538 3.21e-33 134
Msa0130100 AT1G77240.1 26.306 517 327 17 77 555 34 534 3.99e-33 133
Msa0130100 AT1G21530.1 26.004 523 318 18 78 555 38 536 9.34e-33 132
Msa0130100 AT1G66120.1 27.342 523 321 18 82 558 32 541 3.37e-32 131
Msa0130100 AT5G16370.1 25.098 510 337 15 79 555 37 534 6.47e-31 127
Msa0130100 AT1G30520.3 27.292 469 271 16 70 490 14 460 1.05e-29 122
Msa0130100 AT1G30520.4 27.292 469 271 16 70 490 14 460 1.05e-29 122
Msa0130100 AT1G20560.2 26.400 375 252 9 209 570 107 470 1.45e-29 122
Msa0130100 AT3G16910.1 28.042 378 236 14 209 563 195 559 1.08e-28 120
Msa0130100 AT1G75960.1 25.490 510 331 13 84 558 42 537 1.18e-28 120
Msa0130100 AT5G16340.1 24.165 509 343 14 79 555 37 534 4.81e-28 118
Msa0130100 AT3G16170.1 26.862 376 236 11 212 558 235 600 6.26e-27 115
Msa0130100 AT2G17650.1 25.000 520 325 17 84 555 88 590 6.83e-25 109
Msa0130100 AT1G68270.1 25.468 534 294 20 82 558 32 518 3.34e-24 107
Msa0130100 AT1G76290.1 23.917 531 346 17 73 563 23 535 2.65e-23 104
Msa0130100 AT5G27600.1 26.730 318 175 11 212 481 262 569 5.09e-18 88.2
Msa0130100 AT5G36880.2 23.475 541 352 17 76 566 191 719 5.66e-18 88.2
Msa0130100 AT5G36880.6 23.475 541 352 17 76 566 141 669 6.74e-18 87.8
Msa0130100 AT5G36880.3 23.475 541 352 17 76 566 141 669 6.74e-18 87.8
Msa0130100 AT5G36880.5 23.475 541 352 17 76 566 141 669 6.74e-18 87.8
Msa0130100 AT5G36880.1 23.475 541 352 17 76 566 141 669 6.74e-18 87.8
Msa0130100 AT1G49430.1 26.197 355 192 14 196 493 210 551 2.33e-16 83.2
Msa0130100 AT5G35930.4 23.495 515 335 16 84 553 59 559 4.67e-12 69.7
Msa0130100 AT5G35930.1 23.640 478 317 14 110 553 4 467 7.21e-11 65.9

Find 141 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCCAATGTTTCATGTCTA+TGG 0.176321 1_3:+60156869 Msa0130100:CDS
ACCCTTCTCACTTTCTTATA+TGG 0.212821 1_3:-60158276 None:intergenic
ATCAGCTTTCCACATGCTTC+TGG 0.219682 1_3:-60157235 None:intergenic
AAATAAAGCTATGGTGTAAT+TGG 0.225021 1_3:+60158514 Msa0130100:three_prime_UTR
GGACTGATGTTCTTTGGATT+TGG 0.232726 1_3:+60156890 Msa0130100:CDS
GTTGAACTAAGGGCGGTTTA+TGG 0.238290 1_3:+60157151 Msa0130100:CDS
GAATGGTAAATTCCTGTTCT+TGG 0.248189 1_3:-60156173 None:intergenic
GTCTATGGACTGATGTTCTT+TGG 0.250992 1_3:+60156884 Msa0130100:CDS
GTTGAGTACAAGATTGAGTT+TGG 0.273727 1_3:-60156434 None:intergenic
TAACTGCTGCATCAACAATA+AGG 0.276654 1_3:-60157704 None:intergenic
CCAGCAGAGCAGTTTCATTA+TGG 0.284701 1_3:-60156801 None:intergenic
ACCAATTTGTGTAACTCTTC+TGG 0.296211 1_3:-60156575 None:intergenic
GACTGATGTTCTTTGGATTT+GGG 0.298826 1_3:+60156891 Msa0130100:CDS
CTTTGTTGGGATTTCATGTT+TGG 0.301287 1_3:-60156213 None:intergenic
CTCTGTCTCCATTTGATAAC+AGG 0.330041 1_3:+60156138 Msa0130100:CDS
CATGTCATTCAATTTGTTGC+AGG 0.341335 1_3:+60157885 Msa0130100:CDS
CACCATAGCTTTATTTCTTT+AGG 0.341674 1_3:-60158507 None:intergenic
AATCAAGCACAAAGGGTATC+AGG 0.343261 1_3:+60157483 Msa0130100:CDS
CTACGTTTCACTCTCTTTGC+CGG 0.344130 1_3:+60155867 Msa0130100:five_prime_UTR
GCTGCTATACTTTACTCTTC+AGG 0.349123 1_3:+60156728 Msa0130100:CDS
AAAGTGATAGACATTGAAAC+AGG 0.356914 1_3:+60157274 Msa0130100:CDS
AAATGTCAGGAGAGGAAGTT+TGG 0.364108 1_3:+60156087 Msa0130100:exon
CTACATAGTTGAACGAATAA+AGG 0.367598 1_3:+60157456 Msa0130100:CDS
AACTGCTGCATCAACAATAA+GGG 0.374049 1_3:-60157703 None:intergenic
TCAGGTAGCTCCTGCAGAAC+TGG 0.384556 1_3:+60157659 Msa0130100:intron
ACCAGAAGAGTTACACAAAT+TGG 0.386896 1_3:+60156574 Msa0130100:CDS
TCAGTTGAAGAGTTAATAGA+AGG 0.392562 1_3:+60156653 Msa0130100:CDS
GCGAAGCAAGTACATGATTC+AGG 0.393921 1_3:+60156530 Msa0130100:CDS
AGGGTTGAAGATGAAGAAAC+TGG 0.395025 1_3:+60157807 Msa0130100:intron
CACCTGAGCCAACCCTTCTT+AGG 0.395579 1_3:-60157075 None:intergenic
CTTTACTCTTCAGGGACTAC+TGG 0.405377 1_3:+60156737 Msa0130100:CDS
TATTTCCTTGGGTTGAACTA+AGG 0.410515 1_3:+60157140 Msa0130100:CDS
GTTCAGAAAGCTATTTCCTT+GGG 0.412932 1_3:+60157129 Msa0130100:CDS
TGTTGAGATCTGGCTTTGTT+GGG 0.419272 1_3:-60156226 None:intergenic
TCAATTTGTCAGTAAAACTT+TGG 0.420873 1_3:+60158585 Msa0130100:three_prime_UTR
CTGCTATACTTTACTCTTCA+GGG 0.434338 1_3:+60156729 Msa0130100:CDS
CCATAATGAAACTGCTCTGC+TGG 0.435438 1_3:+60156801 Msa0130100:CDS
GTTCCTACAATTCTCACCTC+TGG 0.439013 1_3:+60156608 Msa0130100:CDS
GTGTTGAGATCTGGCTTTGT+TGG 0.439500 1_3:-60156227 None:intergenic
AGCGGCTACGATCCAAGAAC+AGG 0.444190 1_3:+60156161 Msa0130100:CDS
CATTCAATTTGTTGCAGGCC+AGG 0.446682 1_3:+60157890 Msa0130100:CDS
GGATTTGGGTTGTTGTGTGT+TGG 0.451406 1_3:+60156905 Msa0130100:CDS
TTTCTAAGTTGTGAGTAACT+TGG 0.452000 1_3:+60158382 Msa0130100:exon
TTTCAAGCTTAACAAGAGAA+TGG 0.452313 1_3:-60156190 None:intergenic
TTCACCAATGAGTGGATAAC+TGG 0.452438 1_3:-60157019 None:intergenic
GCTATTATGAAAGAATATCT+AGG 0.453902 1_3:+60157346 Msa0130100:CDS
GTGATTTGTCTAGCCTAAGA+AGG 0.457731 1_3:+60157062 Msa0130100:CDS
AGCACAGATTCCAGTTCTGC+AGG 0.460066 1_3:-60157669 None:intergenic
TGGATAACTGGTGGCACTGC+TGG 0.462633 1_3:-60157007 None:intergenic
AGAGTTGCAGTGTTGAGATC+TGG 0.462977 1_3:-60156236 None:intergenic
CTCACCTCTGGGACATCTGA+TGG 0.463000 1_3:+60156620 Msa0130100:CDS
GTCCCAGAGGTGAGAATTGT+AGG 0.467301 1_3:-60156611 None:intergenic
TTACACAAATTGGTCCCAAC+TGG 0.471916 1_3:+60156584 Msa0130100:CDS
GCATATGTGGTGAGGGCAGC+TGG 0.475126 1_3:+60157843 Msa0130100:CDS
CAACAATTGATTCAGAAGGT+TGG 0.475182 1_3:+60157389 Msa0130100:CDS
TTCTAAGTTGTGAGTAACTT+GGG 0.479667 1_3:+60158383 Msa0130100:exon
ATGGACTAACAGAAAGTTGT+GGG 0.484025 1_3:+60157170 Msa0130100:CDS
TAGCCGCTCCTGTTATCAAA+TGG 0.484875 1_3:-60156146 None:intergenic
TGGTTGAGGACAGGTGATCT+TGG 0.485306 1_3:+60157409 Msa0130100:CDS
GACATGAAACATTGGAATGA+AGG 0.489639 1_3:-60156864 None:intergenic
AGTTCAGAAAGCTATTTCCT+TGG 0.490058 1_3:+60157128 Msa0130100:CDS
AGGTGCTGCACCTTTGAGTA+AGG 0.492711 1_3:+60157093 Msa0130100:CDS
ATTTCCTTGGGTTGAACTAA+GGG 0.492805 1_3:+60157141 Msa0130100:CDS
CCTTCCTTGAGTGGAGGCAA+AGG 0.493651 1_3:-60157301 None:intergenic
CTTGGTTATATCGATGAGAA+TGG 0.496399 1_3:+60157427 Msa0130100:CDS
CATGGACTTGAGATTAGAAA+AGG 0.499367 1_3:+60156389 Msa0130100:CDS
TTCACCAGATTCTTCTTCAA+CGG 0.499514 1_3:-60156114 None:intergenic
AGCCTAAGAAGGGTTGGCTC+AGG 0.509742 1_3:+60157073 Msa0130100:CDS
AGGAGCTAATCAAGCACAAA+GGG 0.511589 1_3:+60157476 Msa0130100:CDS
TGACTTAGTATTGAGAGGGT+TGG 0.511924 1_3:-60156501 None:intergenic
CACTCAAGGAAGGAGAGTTA+TGG 0.515549 1_3:+60157311 Msa0130100:CDS
TCATCCGTTGAAGAAGAATC+TGG 0.516179 1_3:+60156110 Msa0130100:CDS
GCCACCAGTTATCCACTCAT+TGG 0.516190 1_3:+60157015 Msa0130100:CDS
TGCTTAGCTGTGTTATCAGT+TGG 0.518737 1_3:+60156461 Msa0130100:CDS
GTGAAACATGCTAGCAAAGA+TGG 0.522574 1_3:+60157037 Msa0130100:CDS
TTGATTCAGAAGGTTGGTTG+AGG 0.524384 1_3:+60157395 Msa0130100:CDS
TATCAGCAAGATCTCCTTGT+GGG 0.530117 1_3:-60156274 None:intergenic
TCAGAAGGTTGGTTGAGGAC+AGG 0.530736 1_3:+60157400 Msa0130100:CDS
TGATAACACAGCTAAGCATA+TGG 0.534053 1_3:-60156456 None:intergenic
GAAGCCTTTGCCTCCACTCA+AGG 0.535824 1_3:+60157297 Msa0130100:CDS
TTCCTACAATTCTCACCTCT+GGG 0.539856 1_3:+60156609 Msa0130100:CDS
TTTCTGACTTAGTATTGAGA+GGG 0.544569 1_3:-60156505 None:intergenic
ACTAATCATAGTGTAACTTA+CGG 0.545558 1_3:+60156323 Msa0130100:CDS
CATGGTACAAAGCTGTTGCA+AGG 0.547277 1_3:-60156370 None:intergenic
AGAATTGTAGGAACTCCAGT+TGG 0.555282 1_3:-60156599 None:intergenic
AGTATACCGAGGTCAGCTGC+TGG 0.555861 1_3:+60158312 Msa0130100:CDS
CATAACTCTCCTTCCTTGAG+TGG 0.558671 1_3:-60157310 None:intergenic
TTTGAGTAAGGAAATGTCAC+TGG 0.559342 1_3:+60157105 Msa0130100:CDS
TGGTTTAGTATAAACTGAGT+TGG 0.560230 1_3:+60158605 Msa0130100:three_prime_UTR
ACCAATGGCATATGTGGTGA+GGG 0.561041 1_3:+60157836 Msa0130100:CDS
AGCCTAAAGAAATAAAGCTA+TGG 0.561824 1_3:+60158505 Msa0130100:three_prime_UTR
GCCCTCACCACATATGCCAT+TGG 0.565672 1_3:-60157837 None:intergenic
TTGTCTAGCCTAAGAAGGGT+TGG 0.570054 1_3:+60157067 Msa0130100:CDS
GAATTGTAGGAACTCCAGTT+GGG 0.571691 1_3:-60156598 None:intergenic
AGAATATCTAGGAAATATTG+AGG 0.571721 1_3:+60157357 Msa0130100:CDS
CTCACAACTTAGAAACAAGC+TGG 0.574328 1_3:-60158374 None:intergenic
AGAAGCCGCCTTATATCAGA+AGG 0.579500 1_3:+60158464 Msa0130100:three_prime_UTR
ATTTCTGACTTAGTATTGAG+AGG 0.580354 1_3:-60156506 None:intergenic
CCTTTGCCTCCACTCAAGGA+AGG 0.582843 1_3:+60157301 Msa0130100:CDS
CTTGCAACAGCTTTGTACCA+TGG 0.587018 1_3:+60156371 Msa0130100:CDS
TGATTTGTCTAGCCTAAGAA+GGG 0.587869 1_3:+60157063 Msa0130100:CDS
ATCTTGCCAGCAGCTGACCT+CGG 0.591787 1_3:-60158318 None:intergenic
TCCATATAAGAAAGTGAGAA+GGG 0.594862 1_3:+60158275 Msa0130100:CDS
CTCCATATAAGAAAGTGAGA+AGG 0.595321 1_3:+60158274 Msa0130100:CDS
TACCAATGGCATATGTGGTG+AGG 0.595660 1_3:+60157835 Msa0130100:CDS
ACTGCAACAATTGATTCAGA+AGG 0.598082 1_3:+60157385 Msa0130100:CDS
AGTATTGAGAGGGTTGGCTG+TGG 0.599920 1_3:-60156495 None:intergenic
ACTGCTGCATCAACAATAAG+GGG 0.600356 1_3:-60157702 None:intergenic
AGTGACATTTCCTTACTCAA+AGG 0.602475 1_3:-60157103 None:intergenic
AACTCTCCTTCCTTGAGTGG+AGG 0.608300 1_3:-60157307 None:intergenic
TCCTTGGGTTGAACTAAGGG+CGG 0.609969 1_3:+60157144 Msa0130100:CDS
GCAGATACCAATGGCATATG+TGG 0.613364 1_3:+60157830 Msa0130100:CDS
AGTCCATAGACATGAAACAT+TGG 0.615670 1_3:-60156872 None:intergenic
AAGGAGCTAATCAAGCACAA+AGG 0.617428 1_3:+60157475 Msa0130100:CDS
TATGGACTAACAGAAAGTTG+TGG 0.620986 1_3:+60157169 Msa0130100:CDS
TTACCCCTTCTGATATAAGG+CGG 0.622594 1_3:-60158469 None:intergenic
ATGGTACAAAGCTGTTGCAA+GGG 0.627880 1_3:-60156369 None:intergenic
GGGACTACTGGAGTAAGCAA+AGG 0.641617 1_3:+60156749 Msa0130100:CDS
ACCGCCCTTAGTTCAACCCA+AGG 0.642066 1_3:-60157145 None:intergenic
AGAGTTGCCACATGTTCATG+TGG 0.646575 1_3:+60156691 Msa0130100:CDS
GTGCTATCACAGTTCCCACA+AGG 0.647014 1_3:+60156260 Msa0130100:CDS
ACCAATGAGTGGATAACTGG+TGG 0.649415 1_3:-60157016 None:intergenic
GGGTTGAAGATGAAGAAACT+GGG 0.650548 1_3:+60157808 Msa0130100:intron
AAAGCTCATCCAGAAGCATG+TGG 0.657133 1_3:+60157226 Msa0130100:CDS
GAAACATTGGAATGAAGGCG+AGG 0.657962 1_3:-60156859 None:intergenic
AAGCCGCCTTATATCAGAAG+GGG 0.658392 1_3:+60158466 Msa0130100:three_prime_UTR
AGAAACTGGGCAGATACCAA+TGG 0.659161 1_3:+60157821 Msa0130100:CDS
CTGCAACAAATTGAATGACA+TGG 0.661163 1_3:-60157884 None:intergenic
TAGCATGTTTCACCAATGAG+TGG 0.661752 1_3:-60157027 None:intergenic
TCTCCATTTGATAACAGGAG+CGG 0.663420 1_3:+60156143 Msa0130100:CDS
TGGACTAACAGAAAGTTGTG+GGG 0.664005 1_3:+60157171 Msa0130100:CDS
CTTCTCGATAGCGACAAGCA+TGG 0.668123 1_3:-60156966 None:intergenic
GAAGCCGCCTTATATCAGAA+GGG 0.670001 1_3:+60158465 Msa0130100:three_prime_UTR
GGACTAACAGAAAGTTGTGG+GGG 0.674978 1_3:+60157172 Msa0130100:CDS
GATTGCTCCACATGAACATG+TGG 0.679529 1_3:-60156698 None:intergenic
GTATCAGCAAGATCTCCTTG+TGG 0.682118 1_3:-60156275 None:intergenic
ATGAAGTGGAGTTCAAAGCG+AGG 0.696930 1_3:+60158441 Msa0130100:three_prime_UTR
GGAGAGTTATGGTTGAAAAG+CGG 0.697534 1_3:+60157322 Msa0130100:CDS
AAGTGATAGACATTGAAACA+GGG 0.705961 1_3:+60157275 Msa0130100:CDS
ATGTCAATGCTAATGTAGTG+TGG 0.718143 1_3:-60156042 None:intergenic
CTTTCCATCAGATGTCCCAG+AGG 0.738092 1_3:-60156624 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTTAACTTAAATAATC+AGG - chr1_3:60158033-60158052 None:intergenic 10.0%
!! AGTAAATTACTTTAATTCTA+AGG + chr1_3:60157577-60157596 Msa0130100:intron 15.0%
!!! AGTTTTTATTAATACATATG+TGG + chr1_3:60157626-60157645 Msa0130100:intron 15.0%
!!! TTTGTATTTTAAACCTAATT+AGG - chr1_3:60158076-60158095 None:intergenic 15.0%
!! ATTGAAACTGAAACTATTTA+TGG - chr1_3:60157518-60157537 None:intergenic 20.0%
!! ATAAATAGTTTCAGTTTCAA+TGG + chr1_3:60157517-60157536 Msa0130100:intron 20.0%
!! ATGAAAATAAGAGTGAAAAT+AGG - chr1_3:60157749-60157768 None:intergenic 20.0%
!! AGAGAAATTAAAATGACTAT+AGG - chr1_3:60158210-60158229 None:intergenic 20.0%
!!! AAATAAATCTGAGAATTTTC+AGG + chr1_3:60158248-60158267 Msa0130100:intron 20.0%
!!! TTTTTCTTCAATTAATGAAG+TGG + chr1_3:60158427-60158446 Msa0130100:three_prime_UTR 20.0%
!! TTTTAGTCCTGAAATTTTTG+TGG - chr1_3:60155893-60155912 None:intergenic 25.0%
!! ACATATCTATCAATTTTGCA+GGG + chr1_3:60156061-60156080 Msa0130100:five_prime_UTR 25.0%
! ACTAATCATAGTGTAACTTA+CGG + chr1_3:60156323-60156342 Msa0130100:CDS 25.0%
! GCTATTATGAAAGAATATCT+AGG + chr1_3:60157346-60157365 Msa0130100:CDS 25.0%
! AGAATATCTAGGAAATATTG+AGG + chr1_3:60157357-60157376 Msa0130100:CDS 25.0%
! AAAATAAGAGTGAAAATAGG+CGG - chr1_3:60157746-60157765 None:intergenic 25.0%
!!! TTATTTTACTGCATGAAATC+AGG - chr1_3:60157932-60157951 None:intergenic 25.0%
!! TTGTAATCATTGTTGCTTAT+TGG + chr1_3:60157962-60157981 Msa0130100:intron 25.0%
! AAATAAAGCTATGGTGTAAT+TGG + chr1_3:60158514-60158533 Msa0130100:three_prime_UTR 25.0%
! TCAATTTGTCAGTAAAACTT+TGG + chr1_3:60158585-60158604 Msa0130100:three_prime_UTR 25.0%
ACTACTATGTCAGTTGTATA+AGG - chr1_3:60155933-60155952 None:intergenic 30.0%
!! GATTCTTTTCGTAAAGTACT+AGG - chr1_3:60155972-60155991 None:intergenic 30.0%
CAGCATCTATAGTATATACA+AGG - chr1_3:60155994-60156013 None:intergenic 30.0%
! GACATATCTATCAATTTTGC+AGG + chr1_3:60156060-60156079 Msa0130100:five_prime_UTR 30.0%
TTTCAAGCTTAACAAGAGAA+TGG - chr1_3:60156193-60156212 None:intergenic 30.0%
! TTTCTGACTTAGTATTGAGA+GGG - chr1_3:60156508-60156527 None:intergenic 30.0%
! ATTTCTGACTTAGTATTGAG+AGG - chr1_3:60156509-60156528 None:intergenic 30.0%
TCAGTTGAAGAGTTAATAGA+AGG + chr1_3:60156653-60156672 Msa0130100:CDS 30.0%
ATTCCAATGTTTCATGTCTA+TGG + chr1_3:60156869-60156888 Msa0130100:CDS 30.0%
!! ATCACTTTTGCACAAAAAGT+TGG - chr1_3:60157262-60157281 None:intergenic 30.0%
AAAGTGATAGACATTGAAAC+AGG + chr1_3:60157274-60157293 Msa0130100:CDS 30.0%
AAGTGATAGACATTGAAACA+GGG + chr1_3:60157275-60157294 Msa0130100:CDS 30.0%
CTACATAGTTGAACGAATAA+AGG + chr1_3:60157456-60157475 Msa0130100:CDS 30.0%
! CTAAGGTATCAAATTGAGTT+TGG + chr1_3:60157594-60157613 Msa0130100:intron 30.0%
GTATCAAATTGAGTTTGGAA+CGG + chr1_3:60157599-60157618 Msa0130100:intron 30.0%
! AATCATTGTTGCTTATTGGT+TGG + chr1_3:60157966-60157985 Msa0130100:intron 30.0%
TATTCTGACTTCACCTAATT+AGG + chr1_3:60158060-60158079 Msa0130100:intron 30.0%
ATTGTTGGATGAATGTCATA+TGG - chr1_3:60158112-60158131 None:intergenic 30.0%
AGTTATTGCATCTAGATTGT+TGG - chr1_3:60158127-60158146 None:intergenic 30.0%
!! TAAATCTGAGAATTTTCAGG+TGG + chr1_3:60158251-60158270 Msa0130100:intron 30.0%
TCCATATAAGAAAGTGAGAA+GGG + chr1_3:60158275-60158294 Msa0130100:CDS 30.0%
TTTCTAAGTTGTGAGTAACT+TGG + chr1_3:60158382-60158401 Msa0130100:exon 30.0%
TTCTAAGTTGTGAGTAACTT+GGG + chr1_3:60158383-60158402 Msa0130100:exon 30.0%
! ATTTTACCCCTTCTGATATA+AGG - chr1_3:60158475-60158494 None:intergenic 30.0%
AGCCTAAAGAAATAAAGCTA+TGG + chr1_3:60158505-60158524 Msa0130100:three_prime_UTR 30.0%
!! CACCATAGCTTTATTTCTTT+AGG - chr1_3:60158510-60158529 None:intergenic 30.0%
TGGTTTAGTATAAACTGAGT+TGG + chr1_3:60158605-60158624 Msa0130100:three_prime_UTR 30.0%
ATGTCAATGCTAATGTAGTG+TGG - chr1_3:60156045-60156064 None:intergenic 35.0%
! TTTTGCAGGGAAAAAATGTC+AGG + chr1_3:60156074-60156093 Msa0130100:exon 35.0%
TTCACCAGATTCTTCTTCAA+CGG - chr1_3:60156117-60156136 None:intergenic 35.0%
GAATGGTAAATTCCTGTTCT+TGG - chr1_3:60156176-60156195 None:intergenic 35.0%
CTTTGTTGGGATTTCATGTT+TGG - chr1_3:60156216-60156235 None:intergenic 35.0%
! CTTTTCTAATCTCAAGTCCA+TGG - chr1_3:60156391-60156410 None:intergenic 35.0%
CATGGACTTGAGATTAGAAA+AGG + chr1_3:60156389-60156408 Msa0130100:CDS 35.0%
GTTGAGTACAAGATTGAGTT+TGG - chr1_3:60156437-60156456 None:intergenic 35.0%
TGATAACACAGCTAAGCATA+TGG - chr1_3:60156459-60156478 None:intergenic 35.0%
ACCAGAAGAGTTACACAAAT+TGG + chr1_3:60156574-60156593 Msa0130100:CDS 35.0%
ACCAATTTGTGTAACTCTTC+TGG - chr1_3:60156578-60156597 None:intergenic 35.0%
CTGCTATACTTTACTCTTCA+GGG + chr1_3:60156729-60156748 Msa0130100:CDS 35.0%
GACATGAAACATTGGAATGA+AGG - chr1_3:60156867-60156886 None:intergenic 35.0%
AGTCCATAGACATGAAACAT+TGG - chr1_3:60156875-60156894 None:intergenic 35.0%
GACTGATGTTCTTTGGATTT+GGG + chr1_3:60156891-60156910 Msa0130100:CDS 35.0%
! TGATTTGTCTAGCCTAAGAA+GGG + chr1_3:60157063-60157082 Msa0130100:CDS 35.0%
AGTGACATTTCCTTACTCAA+AGG - chr1_3:60157106-60157125 None:intergenic 35.0%
TTTGAGTAAGGAAATGTCAC+TGG + chr1_3:60157105-60157124 Msa0130100:CDS 35.0%
! AGTTCAGAAAGCTATTTCCT+TGG + chr1_3:60157128-60157147 Msa0130100:CDS 35.0%
! GTTCAGAAAGCTATTTCCTT+GGG + chr1_3:60157129-60157148 Msa0130100:CDS 35.0%
!! TATTTCCTTGGGTTGAACTA+AGG + chr1_3:60157140-60157159 Msa0130100:CDS 35.0%
!! ATTTCCTTGGGTTGAACTAA+GGG + chr1_3:60157141-60157160 Msa0130100:CDS 35.0%
! TATGGACTAACAGAAAGTTG+TGG + chr1_3:60157169-60157188 Msa0130100:CDS 35.0%
! ATGGACTAACAGAAAGTTGT+GGG + chr1_3:60157170-60157189 Msa0130100:CDS 35.0%
! ACTGCAACAATTGATTCAGA+AGG + chr1_3:60157385-60157404 Msa0130100:CDS 35.0%
! CAACAATTGATTCAGAAGGT+TGG + chr1_3:60157389-60157408 Msa0130100:CDS 35.0%
CTTGGTTATATCGATGAGAA+TGG + chr1_3:60157427-60157446 Msa0130100:CDS 35.0%
AACTGCTGCATCAACAATAA+GGG - chr1_3:60157706-60157725 None:intergenic 35.0%
TAACTGCTGCATCAACAATA+AGG - chr1_3:60157707-60157726 None:intergenic 35.0%
ATGTGGACAAAAAATGTTGC+AGG + chr1_3:60157787-60157806 Msa0130100:intron 35.0%
TGTGGACAAAAAATGTTGCA+GGG + chr1_3:60157788-60157807 Msa0130100:intron 35.0%
CTGCAACAAATTGAATGACA+TGG - chr1_3:60157887-60157906 None:intergenic 35.0%
!! CATGTCATTCAATTTGTTGC+AGG + chr1_3:60157885-60157904 Msa0130100:CDS 35.0%
CTCCATATAAGAAAGTGAGA+AGG + chr1_3:60158274-60158293 Msa0130100:CDS 35.0%
ACCCTTCTCACTTTCTTATA+TGG - chr1_3:60158279-60158298 None:intergenic 35.0%
!! GTTTTATTGACAGTATACCG+AGG + chr1_3:60158301-60158320 Msa0130100:CDS 35.0%
! AGTCCTGAAATTTTTGTGGC+CGG - chr1_3:60155889-60155908 None:intergenic 40.0%
AAATGTCAGGAGAGGAAGTT+TGG + chr1_3:60156087-60156106 Msa0130100:exon 40.0%
TCATCCGTTGAAGAAGAATC+TGG + chr1_3:60156110-60156129 Msa0130100:CDS 40.0%
! CTCTGTCTCCATTTGATAAC+AGG + chr1_3:60156138-60156157 Msa0130100:CDS 40.0%
! TCTCCATTTGATAACAGGAG+CGG + chr1_3:60156143-60156162 Msa0130100:CDS 40.0%
!! TGTTGAGATCTGGCTTTGTT+GGG - chr1_3:60156229-60156248 None:intergenic 40.0%
TATCAGCAAGATCTCCTTGT+GGG - chr1_3:60156277-60156296 None:intergenic 40.0%
ATGGTACAAAGCTGTTGCAA+GGG - chr1_3:60156372-60156391 None:intergenic 40.0%
TGCTTAGCTGTGTTATCAGT+TGG + chr1_3:60156461-60156480 Msa0130100:CDS 40.0%
! TGACTTAGTATTGAGAGGGT+TGG - chr1_3:60156504-60156523 None:intergenic 40.0%
TTACACAAATTGGTCCCAAC+TGG + chr1_3:60156584-60156603 Msa0130100:CDS 40.0%
GAATTGTAGGAACTCCAGTT+GGG - chr1_3:60156601-60156620 None:intergenic 40.0%
AGAATTGTAGGAACTCCAGT+TGG - chr1_3:60156602-60156621 None:intergenic 40.0%
TTCCTACAATTCTCACCTCT+GGG + chr1_3:60156609-60156628 Msa0130100:CDS 40.0%
GCTGCTATACTTTACTCTTC+AGG + chr1_3:60156728-60156747 Msa0130100:CDS 40.0%
! GTCTATGGACTGATGTTCTT+TGG + chr1_3:60156884-60156903 Msa0130100:CDS 40.0%
GGACTGATGTTCTTTGGATT+TGG + chr1_3:60156890-60156909 Msa0130100:CDS 40.0%
TTCACCAATGAGTGGATAAC+TGG - chr1_3:60157022-60157041 None:intergenic 40.0%
TAGCATGTTTCACCAATGAG+TGG - chr1_3:60157030-60157049 None:intergenic 40.0%
GTGAAACATGCTAGCAAAGA+TGG + chr1_3:60157037-60157056 Msa0130100:CDS 40.0%
! GTGATTTGTCTAGCCTAAGA+AGG + chr1_3:60157062-60157081 Msa0130100:CDS 40.0%
TGGACTAACAGAAAGTTGTG+GGG + chr1_3:60157171-60157190 Msa0130100:CDS 40.0%
GGAGAGTTATGGTTGAAAAG+CGG + chr1_3:60157322-60157341 Msa0130100:CDS 40.0%
! TTGATTCAGAAGGTTGGTTG+AGG + chr1_3:60157395-60157414 Msa0130100:CDS 40.0%
AAGGAGCTAATCAAGCACAA+AGG + chr1_3:60157475-60157494 Msa0130100:CDS 40.0%
AGGAGCTAATCAAGCACAAA+GGG + chr1_3:60157476-60157495 Msa0130100:CDS 40.0%
!! AATCAAGCACAAAGGGTATC+AGG + chr1_3:60157483-60157502 Msa0130100:CDS 40.0%
! ATATGTGGATTGCTTGTGTC+AGG + chr1_3:60157641-60157660 Msa0130100:intron 40.0%
ACTGCTGCATCAACAATAAG+GGG - chr1_3:60157705-60157724 None:intergenic 40.0%
TAAGAGTGAAAATAGGCGGA+GGG - chr1_3:60157742-60157761 None:intergenic 40.0%
ATAAGAGTGAAAATAGGCGG+AGG - chr1_3:60157743-60157762 None:intergenic 40.0%
CTCTCTGTGACTTTCTTATG+TGG + chr1_3:60157770-60157789 Msa0130100:intron 40.0%
AGGGTTGAAGATGAAGAAAC+TGG + chr1_3:60157807-60157826 Msa0130100:intron 40.0%
GGGTTGAAGATGAAGAAACT+GGG + chr1_3:60157808-60157827 Msa0130100:intron 40.0%
CTATAGGAAAACGAGAAAGC+AGG - chr1_3:60158194-60158213 None:intergenic 40.0%
CTCACAACTTAGAAACAAGC+TGG - chr1_3:60158377-60158396 None:intergenic 40.0%
TTACCCCTTCTGATATAAGG+CGG - chr1_3:60158472-60158491 None:intergenic 40.0%
CTACGTTTCACTCTCTTTGC+CGG + chr1_3:60155867-60155886 Msa0130100:five_prime_UTR 45.0%
TTGCCGGCCACAAAAATTTC+AGG + chr1_3:60155883-60155902 Msa0130100:five_prime_UTR 45.0%
GTGTGGCTGTGGCAAAATAA+GGG - chr1_3:60156028-60156047 None:intergenic 45.0%
AGTGTGGCTGTGGCAAAATA+AGG - chr1_3:60156029-60156048 None:intergenic 45.0%
ATGCTAATGTAGTGTGGCTG+TGG - chr1_3:60156039-60156058 None:intergenic 45.0%
CAGGGAAAAAATGTCAGGAG+AGG + chr1_3:60156079-60156098 Msa0130100:exon 45.0%
TAGCCGCTCCTGTTATCAAA+TGG - chr1_3:60156149-60156168 None:intergenic 45.0%
!! GTGTTGAGATCTGGCTTTGT+TGG - chr1_3:60156230-60156249 None:intergenic 45.0%
! AGAGTTGCAGTGTTGAGATC+TGG - chr1_3:60156239-60156258 None:intergenic 45.0%
GTATCAGCAAGATCTCCTTG+TGG - chr1_3:60156278-60156297 None:intergenic 45.0%
CATGGTACAAAGCTGTTGCA+AGG - chr1_3:60156373-60156392 None:intergenic 45.0%
! CTTGCAACAGCTTTGTACCA+TGG + chr1_3:60156371-60156390 Msa0130100:CDS 45.0%
GCGAAGCAAGTACATGATTC+AGG + chr1_3:60156530-60156549 Msa0130100:CDS 45.0%
GTTCCTACAATTCTCACCTC+TGG + chr1_3:60156608-60156627 Msa0130100:CDS 45.0%
AGAGTTGCCACATGTTCATG+TGG + chr1_3:60156691-60156710 Msa0130100:CDS 45.0%
GATTGCTCCACATGAACATG+TGG - chr1_3:60156701-60156720 None:intergenic 45.0%
CTTTACTCTTCAGGGACTAC+TGG + chr1_3:60156737-60156756 Msa0130100:CDS 45.0%
CCAGCAGAGCAGTTTCATTA+TGG - chr1_3:60156804-60156823 None:intergenic 45.0%
CCATAATGAAACTGCTCTGC+TGG + chr1_3:60156801-60156820 Msa0130100:CDS 45.0%
GAAACATTGGAATGAAGGCG+AGG - chr1_3:60156862-60156881 None:intergenic 45.0%
!! GGATTTGGGTTGTTGTGTGT+TGG + chr1_3:60156905-60156924 Msa0130100:CDS 45.0%
ACCAATGAGTGGATAACTGG+TGG - chr1_3:60157019-60157038 None:intergenic 45.0%
TTGTCTAGCCTAAGAAGGGT+TGG + chr1_3:60157067-60157086 Msa0130100:CDS 45.0%
GTTGAACTAAGGGCGGTTTA+TGG + chr1_3:60157151-60157170 Msa0130100:CDS 45.0%
GGACTAACAGAAAGTTGTGG+GGG + chr1_3:60157172-60157191 Msa0130100:CDS 45.0%
! AAAGCTCATCCAGAAGCATG+TGG + chr1_3:60157226-60157245 Msa0130100:CDS 45.0%
ATCAGCTTTCCACATGCTTC+TGG - chr1_3:60157238-60157257 None:intergenic 45.0%
CATAACTCTCCTTCCTTGAG+TGG - chr1_3:60157313-60157332 None:intergenic 45.0%
CACTCAAGGAAGGAGAGTTA+TGG + chr1_3:60157311-60157330 Msa0130100:CDS 45.0%
AAGAGTGAAAATAGGCGGAG+GGG - chr1_3:60157741-60157760 None:intergenic 45.0%
AGAAACTGGGCAGATACCAA+TGG + chr1_3:60157821-60157840 Msa0130100:CDS 45.0%
GCAGATACCAATGGCATATG+TGG + chr1_3:60157830-60157849 Msa0130100:CDS 45.0%
TACCAATGGCATATGTGGTG+AGG + chr1_3:60157835-60157854 Msa0130100:CDS 45.0%
ACCAATGGCATATGTGGTGA+GGG + chr1_3:60157836-60157855 Msa0130100:CDS 45.0%
!! CATTCAATTTGTTGCAGGCC+AGG + chr1_3:60157890-60157909 Msa0130100:CDS 45.0%
ATGAAATCAGGAGCCAAGAG+TGG - chr1_3:60157920-60157939 None:intergenic 45.0%
GGAGCTAAGAGAAGCAGAAA+TGG - chr1_3:60158173-60158192 None:intergenic 45.0%
ATGAAGTGGAGTTCAAAGCG+AGG + chr1_3:60158441-60158460 Msa0130100:three_prime_UTR 45.0%
AGAAGCCGCCTTATATCAGA+AGG + chr1_3:60158464-60158483 Msa0130100:three_prime_UTR 45.0%
GAAGCCGCCTTATATCAGAA+GGG + chr1_3:60158465-60158484 Msa0130100:three_prime_UTR 45.0%
AAGCCGCCTTATATCAGAAG+GGG + chr1_3:60158466-60158485 Msa0130100:three_prime_UTR 45.0%
GTGCTATCACAGTTCCCACA+AGG + chr1_3:60156260-60156279 Msa0130100:CDS 50.0%
!! AGTATTGAGAGGGTTGGCTG+TGG - chr1_3:60156498-60156517 None:intergenic 50.0%
GTCCCAGAGGTGAGAATTGT+AGG - chr1_3:60156614-60156633 None:intergenic 50.0%
CTTTCCATCAGATGTCCCAG+AGG - chr1_3:60156627-60156646 None:intergenic 50.0%
GGGACTACTGGAGTAAGCAA+AGG + chr1_3:60156749-60156768 Msa0130100:CDS 50.0%
CTTCTCGATAGCGACAAGCA+TGG - chr1_3:60156969-60156988 None:intergenic 50.0%
GCCACCAGTTATCCACTCAT+TGG + chr1_3:60157015-60157034 Msa0130100:CDS 50.0%
! AGGTGCTGCACCTTTGAGTA+AGG + chr1_3:60157093-60157112 Msa0130100:CDS 50.0%
!! TCCTTGGGTTGAACTAAGGG+CGG + chr1_3:60157144-60157163 Msa0130100:CDS 50.0%
! TGTGGGGGAGCTACGTTTTT+CGG + chr1_3:60157187-60157206 Msa0130100:CDS 50.0%
AACTCTCCTTCCTTGAGTGG+AGG - chr1_3:60157310-60157329 None:intergenic 50.0%
! TCAGAAGGTTGGTTGAGGAC+AGG + chr1_3:60157400-60157419 Msa0130100:CDS 50.0%
TGGTTGAGGACAGGTGATCT+TGG + chr1_3:60157409-60157428 Msa0130100:CDS 50.0%
AGCACAGATTCCAGTTCTGC+AGG - chr1_3:60157672-60157691 None:intergenic 50.0%
!! CAATTTGTTGCAGGCCAGGT+AGG + chr1_3:60157894-60157913 Msa0130100:intron 50.0%
AGCGGCTACGATCCAAGAAC+AGG + chr1_3:60156161-60156180 Msa0130100:CDS 55.0%
!! CTCACCTCTGGGACATCTGA+TGG + chr1_3:60156620-60156639 Msa0130100:CDS 55.0%
TGGATAACTGGTGGCACTGC+TGG - chr1_3:60157010-60157029 None:intergenic 55.0%
!! AGCCTAAGAAGGGTTGGCTC+AGG + chr1_3:60157073-60157092 Msa0130100:CDS 55.0%
CACCTGAGCCAACCCTTCTT+AGG - chr1_3:60157078-60157097 None:intergenic 55.0%
ACCGCCCTTAGTTCAACCCA+AGG - chr1_3:60157148-60157167 None:intergenic 55.0%
! GTGGGGGAGCTACGTTTTTC+GGG + chr1_3:60157188-60157207 Msa0130100:CDS 55.0%
!!! GGGAGCTACGTTTTTCGGGT+CGG + chr1_3:60157192-60157211 Msa0130100:CDS 55.0%
GAAGCCTTTGCCTCCACTCA+AGG + chr1_3:60157297-60157316 Msa0130100:CDS 55.0%
CCTTCCTTGAGTGGAGGCAA+AGG - chr1_3:60157304-60157323 None:intergenic 55.0%
CCTTTGCCTCCACTCAAGGA+AGG + chr1_3:60157301-60157320 Msa0130100:CDS 55.0%
TCAGGTAGCTCCTGCAGAAC+TGG + chr1_3:60157659-60157678 Msa0130100:intron 55.0%
AATAGGCGGAGGGGATCGTA+CGG - chr1_3:60157732-60157751 None:intergenic 55.0%
GCCCTCACCACATATGCCAT+TGG - chr1_3:60157840-60157859 None:intergenic 55.0%
AGTATACCGAGGTCAGCTGC+TGG + chr1_3:60158312-60158331 Msa0130100:CDS 55.0%
ATCTTGCCAGCAGCTGACCT+CGG - chr1_3:60158321-60158340 None:intergenic 55.0%
GCATATGTGGTGAGGGCAGC+TGG + chr1_3:60157843-60157862 Msa0130100:CDS 60.0%
CAGGCCAGGTAGGCCACTCT+TGG + chr1_3:60157904-60157923 Msa0130100:intron 65.0%
GGAGCCAAGAGTGGCCTACC+TGG - chr1_3:60157911-60157930 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 60155848 60158637 60155848 ID=Msa0130100;Name=Msa0130100
chr1_3 mRNA 60155848 60158637 60155848 ID=Msa0130100-mRNA-1;Parent=Msa0130100;Name=Msa0130100-mRNA-1;_AED=0.06;_eAED=0.06;_QI=135|1|1|1|0.75|0.6|5|242|570
chr1_3 exon 60155848 60155935 60155848 ID=Msa0130100-mRNA-1:exon:19704;Parent=Msa0130100-mRNA-1
chr1_3 exon 60156042 60157504 60156042 ID=Msa0130100-mRNA-1:exon:19705;Parent=Msa0130100-mRNA-1
chr1_3 exon 60157663 60157730 60157663 ID=Msa0130100-mRNA-1:exon:19706;Parent=Msa0130100-mRNA-1
chr1_3 exon 60157809 60157911 60157809 ID=Msa0130100-mRNA-1:exon:19707;Parent=Msa0130100-mRNA-1
chr1_3 exon 60158270 60158637 60158270 ID=Msa0130100-mRNA-1:exon:19708;Parent=Msa0130100-mRNA-1
chr1_3 five_prime_UTR 60155848 60155935 60155848 ID=Msa0130100-mRNA-1:five_prime_utr;Parent=Msa0130100-mRNA-1
chr1_3 five_prime_UTR 60156042 60156088 60156042 ID=Msa0130100-mRNA-1:five_prime_utr;Parent=Msa0130100-mRNA-1
chr1_3 CDS 60156089 60157504 60156089 ID=Msa0130100-mRNA-1:cds;Parent=Msa0130100-mRNA-1
chr1_3 CDS 60157663 60157730 60157663 ID=Msa0130100-mRNA-1:cds;Parent=Msa0130100-mRNA-1
chr1_3 CDS 60157809 60157911 60157809 ID=Msa0130100-mRNA-1:cds;Parent=Msa0130100-mRNA-1
chr1_3 CDS 60158270 60158395 60158270 ID=Msa0130100-mRNA-1:cds;Parent=Msa0130100-mRNA-1
chr1_3 three_prime_UTR 60158396 60158637 60158396 ID=Msa0130100-mRNA-1:three_prime_utr;Parent=Msa0130100-mRNA-1
Gene Sequence

>Msa0130100

ATGTCAGGAGAGGAAGTTTGGTCATCCGTTGAAGAAGAATCTGGTGAATCTCTGTCTCCATTTGATAACAGGAGCGGCTACGATCCAAGAACAGGAATTTACCATTCTCTTGTTAAGCTTGAAACCAAACATGAAATCCCAACAAAGCCAGATCTCAACACTGCAACTCTTGTGCTATCACAGTTCCCACAAGGAGATCTTGCTGATACAAGAATTGCATTCATTGACTTAAATACTAATCATAGTGTAACTTACGGTGAGATTCGTCGATCAGTTTACTCCCTTGCAACAGCTTTGTACCATGGACTTGAGATTAGAAAAGGCGACGTCGTGTTTTTATTGTCTCCAAACTCAATCTTGTACTCAACCATATGCTTAGCTGTGTTATCAGTTGGTGCAATTCTTACCACAGCCAACCCTCTCAATACTAAGTCAGAAATCGCGAAGCAAGTACATGATTCAGGTGCTAAACTAGCTATCTCAGCACCAGAAGAGTTACACAAATTGGTCCCAACTGGAGTTCCTACAATTCTCACCTCTGGGACATCTGATGGAAAGTTTTTATCAGTTGAAGAGTTAATAGAAGGCTGCTATGATTCACAAGAGTTGCCACATGTTCATGTGGAGCAATCAGACACTGCTGCTATACTTTACTCTTCAGGGACTACTGGAGTAAGCAAAGGTGTTGTTTTGACACATGCAAATCTCATAACCATAATGAAACTGCTCTGCTGGTCTGCTGATGTATCTACAGCACAAGACGATGTTTTCCTCGCCTTCATTCCAATGTTTCATGTCTATGGACTGATGTTCTTTGGATTTGGGTTGTTGTGTGTTGGTGTTACGACAGTTTTGATGCAAAAATATGATTTTCAAGCCATGCTTGTCGCTATCGAGAAGCACAAAGTTAATAACATACCAGCAGTGCCACCAGTTATCCACTCATTGGTGAAACATGCTAGCAAAGATGGCTGTGATTTGTCTAGCCTAAGAAGGGTTGGCTCAGGTGCTGCACCTTTGAGTAAGGAAATGTCACTGGAGTTCAGAAAGCTATTTCCTTGGGTTGAACTAAGGGCGGTTTATGGACTAACAGAAAGTTGTGGGGGAGCTACGTTTTTCGGGTCGGATAAAGATGCTAAAGCTCATCCAGAAGCATGTGGAAAGCTGATTCCAACTTTTTGTGCAAAAGTGATAGACATTGAAACAGGGAAGCCTTTGCCTCCACTCAAGGAAGGAGAGTTATGGTTGAAAAGCGGTGCTATTATGAAAGAATATCTAGGAAATATTGAGGCAACAACTGCAACAATTGATTCAGAAGGTTGGTTGAGGACAGGTGATCTTGGTTATATCGATGAGAATGGAATTGTCTACATAGTTGAACGAATAAAGGAGCTAATCAAGCACAAAGGGTATCAGGTAGCTCCTGCAGAACTGGAATCTGTGCTGCTAAGTCATCCCCTTATTGTTGATGCAGCAGTTATTCCGGTTGAAGATGAAGAAACTGGGCAGATACCAATGGCATATGTGGTGAGGGCAGCTGGTTCTCAACTATCAGAAGACCATGTCATTCAATTTGTTGCAGGCCAGGTGGCTCCATATAAGAAAGTGAGAAGGGTGAGTTTTATTGACAGTATACCGAGGTCAGCTGCTGGCAAGATATTGCGAAAAGATCTTGTTTTGCAAAGCAAATACCAGCTTGTTTCTAAGTTGTGA
Protein sequence

>Msa0130100

MSGEEVWSSVEEESGESLSPFDNRSGYDPRTGIYHSLVKLETKHEIPTKPDLNTATLVLSQFPQGDLADTRIAFIDLNTNHSVTYGEIRRSVYSLATALYHGLEIRKGDVVFLLSPNSILYSTICLAVLSVGAILTTANPLNTKSEIAKQVHDSGAKLAISAPEELHKLVPTGVPTILTSGTSDGKFLSVEELIEGCYDSQELPHVHVEQSDTAAILYSSGTTGVSKGVVLTHANLITIMKLLCWSADVSTAQDDVFLAFIPMFHVYGLMFFGFGLLCVGVTTVLMQKYDFQAMLVAIEKHKVNNIPAVPPVIHSLVKHASKDGCDLSSLRRVGSGAAPLSKEMSLEFRKLFPWVELRAVYGLTESCGGATFFGSDKDAKAHPEACGKLIPTFCAKVIDIETGKPLPPLKEGELWLKSGAIMKEYLGNIEATTATIDSEGWLRTGDLGYIDENGIVYIVERIKELIKHKGYQVAPAELESVLLSHPLIVDAAVIPVEDEETGQIPMAYVVRAAGSQLSEDHVIQFVAGQVAPYKKVRRVSFIDSIPRSAAGKILRKDLVLQSKYQLVSKL*