AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0251790

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0251790 MtrunA17_Chr2g0310331 89.862 434 44 0 32 465 158 591 0.0 817
Msa0251790 MtrunA17_Chr8g0357411 66.958 457 149 2 4 459 133 588 0.0 623
Msa0251790 MtrunA17_Chr2g0310401 70.824 425 123 1 36 459 7 431 0.0 610
Msa0251790 MtrunA17_Chr4g0023911 49.650 429 213 3 32 458 152 579 3.81e-138 409
Msa0251790 MtrunA17_Chr8g0357231 68.992 258 79 1 203 459 1 258 2.36e-131 380
Msa0251790 MtrunA17_Chr2g0323151 39.540 435 259 3 26 459 48 479 1.36e-100 309
Msa0251790 MtrunA17_Chr2g0323131 38.303 436 264 4 26 459 132 564 1.53e-98 306
Msa0251790 MtrunA17_Chr4g0042111 36.782 435 267 5 28 460 134 562 8.82e-93 291
Msa0251790 MtrunA17_Chr6g0465441 34.038 426 277 4 37 460 130 553 8.77e-87 276
Msa0251790 MtrunA17_Chr7g0237011 34.353 425 277 2 37 460 75 498 1.22e-84 268
Msa0251790 MtrunA17_Chr7g0236961 34.353 425 277 2 37 460 75 498 1.38e-84 268
Msa0251790 MtrunA17_Chr4g0024691 35.446 426 271 4 37 460 138 561 1.55e-84 270
Msa0251790 MtrunA17_Chr7g0236991 34.353 425 277 2 37 460 130 553 6.31e-84 268
Msa0251790 MtrunA17_Chr7g0237001 34.353 425 277 2 37 460 130 553 6.38e-84 268
Msa0251790 MtrunA17_Chr7g0237041 34.353 425 277 2 37 460 130 553 6.38e-84 268
Msa0251790 MtrunA17_Chr7g0237081 34.353 425 277 2 37 460 130 553 6.38e-84 268
Msa0251790 MtrunA17_Chr7g0237051 34.353 425 277 2 37 460 130 553 6.38e-84 268
Msa0251790 MtrunA17_Chr6g0451711 33.482 448 281 5 15 460 125 557 9.50e-84 268
Msa0251790 MtrunA17_Chr6g0465851 32.727 440 290 3 26 460 80 518 1.70e-83 266
Msa0251790 MtrunA17_Chr6g0465911 32.727 440 290 3 26 460 115 553 4.47e-83 266
Msa0251790 MtrunA17_Chr6g0466051 32.727 440 290 3 26 460 115 553 4.92e-83 266
Msa0251790 MtrunA17_Chr3g0082691 33.111 450 295 3 16 460 105 553 2.88e-81 261
Msa0251790 MtrunA17_Chr7g0236971 33.831 402 264 2 60 460 1 401 5.05e-79 251
Msa0251790 MtrunA17_Chr5g0425371 32.706 425 284 2 37 460 130 553 6.98e-78 253
Msa0251790 MtrunA17_Chr7g0217361 41.391 302 171 4 160 457 8 307 1.67e-77 244
Msa0251790 MtrunA17_Chr2g0319051 33.953 430 275 7 36 460 89 514 7.70e-77 249
Msa0251790 MtrunA17_Chr3g0105071 32.629 426 283 4 37 460 134 557 2.29e-76 249
Msa0251790 MtrunA17_Chr8g0336181 33.875 431 276 7 36 460 129 556 1.38e-75 247
Msa0251790 MtrunA17_Chr5g0431571 33.099 426 281 4 37 460 126 549 1.71e-74 244
Msa0251790 MtrunA17_Chr2g0319041 32.037 437 288 5 28 460 126 557 2.15e-74 244
Msa0251790 MtrunA17_Chr5g0431651 31.690 426 287 4 37 460 129 552 1.20e-70 233
Msa0251790 MtrunA17_Chr6g0477121 33.498 406 263 6 35 434 8 412 4.00e-69 226
Msa0251790 MtrunA17_Chr4g0048921 35.484 403 251 5 43 440 155 553 3.32e-68 228
Msa0251790 MtrunA17_Chr3g0108941 33.838 396 247 7 49 434 105 495 5.79e-67 223
Msa0251790 MtrunA17_Chr6g0477111 33.673 392 253 6 49 434 79 469 1.45e-66 221
Msa0251790 MtrunA17_Chr4g0048911 34.314 408 258 5 37 440 141 542 2.99e-66 223
Msa0251790 MtrunA17_Chr3g0105691 33.586 396 248 7 49 434 137 527 3.96e-66 222
Msa0251790 MtrunA17_Chr2g0280581 32.340 470 291 8 10 454 50 517 1.74e-65 219
Msa0251790 MtrunA17_Chr3g0109001 33.333 396 249 7 49 434 105 495 3.17e-65 218
Msa0251790 MtrunA17_Chr3g0109011 33.333 396 249 7 49 434 79 469 4.29e-65 218
Msa0251790 MtrunA17_Chr3g0109021 33.162 389 245 7 56 434 111 494 1.62e-64 216
Msa0251790 MtrunA17_Chr3g0109031 33.247 385 250 6 56 434 111 494 2.47e-64 216
Msa0251790 MtrunA17_Chr3g0108971 33.081 396 250 7 49 434 79 469 6.06e-64 214
Msa0251790 MtrunA17_Chr6g0465931 41.313 259 147 2 26 280 80 337 6.47e-64 210
Msa0251790 MtrunA17_Chr3g0108981 32.481 391 259 4 49 434 80 470 7.98e-64 214
Msa0251790 MtrunA17_Chr3g0108961 32.481 391 259 4 49 434 80 470 7.98e-64 214
Msa0251790 MtrunA17_Chr6g0466111 41.313 259 147 2 26 280 80 337 9.09e-64 209
Msa0251790 MtrunA17_Chr3g0108991 33.081 396 250 7 49 434 104 494 9.45e-64 214
Msa0251790 MtrunA17_Chr3g0108951 33.081 396 250 7 49 434 104 494 9.45e-64 214
Msa0251790 MtrunA17_Chr6g0465981 41.313 259 147 2 26 280 115 372 1.32e-63 210
Msa0251790 MtrunA17_Chr6g0465871 41.313 259 147 2 26 280 115 372 1.32e-63 210
Msa0251790 MtrunA17_Chr6g0466081 41.313 259 147 2 26 280 115 372 1.32e-63 210
Msa0251790 MtrunA17_Chr3g0099031 33.854 384 249 4 56 434 111 494 1.66e-63 214
Msa0251790 MtrunA17_Chr4g0008621 37.884 293 179 2 32 322 134 425 6.08e-63 210
Msa0251790 MtrunA17_Chr7g0237031 43.621 243 136 1 37 279 75 316 1.12e-62 206
Msa0251790 MtrunA17_Chr7g0236951 43.621 243 136 1 37 279 75 316 1.37e-62 206
Msa0251790 MtrunA17_Chr5g0445511 41.667 228 131 2 55 281 1 227 1.97e-59 195
Msa0251790 MtrunA17_Chr8g0357221 59.748 159 63 1 4 162 133 290 1.26e-54 184
Msa0251790 MtrunA17_Chr2g0319071 26.536 407 254 8 56 458 122 487 5.86e-39 147
Msa0251790 MtrunA17_Chr2g0319081 29.373 303 194 7 163 460 140 427 8.44e-38 143
Msa0251790 MtrunA17_Chr3g0101681 29.064 406 246 14 26 398 323 719 1.13e-34 137
Msa0251790 MtrunA17_Chr6g0466031 26.154 260 191 1 202 460 1 260 1.09e-33 127
Msa0251790 MtrunA17_Chr2g0310121 28.610 374 236 11 26 372 322 691 1.32e-33 134
Msa0251790 MtrunA17_Chr2g0281651 33.193 238 150 6 138 372 43 274 1.28e-31 125
Msa0251790 MtrunA17_Chr7g0237061 27.692 260 164 2 202 460 1 237 1.27e-30 118
Msa0251790 MtrunA17_Chr7g0233861 42.963 135 68 1 101 235 33 158 1.15e-28 111
Msa0251790 MtrunA17_Chr7g0237071 43.411 129 72 1 37 165 75 202 2.25e-25 103
Msa0251790 MtrunA17_Chr7g0217351 34.211 152 91 5 17 163 126 273 3.18e-16 79.3
Msa0251790 MtrunA17_Chr5g0445501 27.168 173 123 3 290 460 1 172 5.59e-14 70.1
Msa0251790 MtrunA17_Chr5g0445401 45.238 84 45 1 74 156 5 88 1.67e-13 66.2
Msa0251790 MtrunA17_Chr7g0237021 23.256 172 131 1 290 460 1 172 1.95e-13 68.6
Msa0251790 MtrunA17_Chr7g0236941 23.256 172 131 1 290 460 1 172 1.95e-13 68.6
Msa0251790 MtrunA17_Chr7g0218001 27.962 211 125 5 26 210 398 607 2.86e-13 72.4
Msa0251790 MtrunA17_Chr7g0259881 25.210 238 145 6 26 237 281 511 2.95e-13 72.0
Msa0251790 MtrunA17_Chr7g0218041 26.360 239 148 6 26 237 1 238 5.96e-13 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0251790 AT3G25820.1 48.182 440 214 7 32 460 158 594 3.17e-141 418
Msa0251790 AT3G25830.1 48.182 440 214 7 32 460 158 594 3.17e-141 418
Msa0251790 AT3G25810.1 46.444 450 221 9 28 460 151 597 2.88e-135 402
Msa0251790 AT4G16730.2 43.723 462 247 5 12 460 32 493 2.16e-134 397
Msa0251790 AT4G16730.1 43.723 462 247 5 12 460 78 539 3.92e-134 397
Msa0251790 AT2G24210.1 44.612 464 238 10 13 460 130 590 4.94e-132 394
Msa0251790 AT4G16740.1 45.122 410 217 5 56 460 158 564 1.44e-126 379
Msa0251790 AT3G25820.2 46.595 279 141 4 32 305 158 433 4.18e-84 266
Msa0251790 AT5G23960.2 33.179 431 275 6 36 460 122 545 6.31e-72 237
Msa0251790 AT5G23960.1 33.487 433 273 7 36 460 122 547 4.13e-71 235
Msa0251790 AT1G61680.2 34.085 399 251 5 50 437 152 549 1.09e-70 234
Msa0251790 AT1G61680.1 33.744 406 262 4 38 437 152 556 1.44e-70 234
Msa0251790 AT4G16740.2 46.288 229 119 2 56 280 158 386 2.14e-68 224
Msa0251790 AT1G70080.1 32.802 439 283 7 26 453 167 604 6.79e-63 215
Msa0251790 AT1G31950.1 29.279 444 293 8 31 460 170 606 4.59e-61 210
Msa0251790 AT1G31950.3 29.279 444 293 8 31 460 171 607 5.07e-61 209
Msa0251790 AT3G14520.1 29.186 442 296 5 31 460 168 604 1.42e-60 208
Msa0251790 AT3G25820.4 48.325 209 100 4 32 235 158 363 1.61e-59 200
Msa0251790 AT3G25820.3 48.325 209 100 4 32 235 158 363 1.61e-59 200
Msa0251790 AT3G29410.2 29.412 442 292 8 31 460 65 498 1.21e-57 198
Msa0251790 AT3G29410.1 29.412 442 292 8 31 460 170 603 8.22e-57 198
Msa0251790 AT3G14540.1 28.348 448 292 8 31 460 165 601 5.98e-56 196
Msa0251790 AT1G33750.1 28.409 440 298 7 31 460 171 603 2.63e-54 191
Msa0251790 AT3G32030.1 27.752 436 309 5 31 460 169 604 6.79e-53 187
Msa0251790 AT3G29190.1 29.885 435 299 5 32 460 167 601 7.55e-52 185
Msa0251790 AT4G16740.4 44.390 205 110 2 56 256 158 362 3.87e-51 177
Msa0251790 AT4G16740.3 44.390 205 110 2 56 256 158 362 3.87e-51 177
Msa0251790 AT3G14490.1 27.778 432 296 7 39 460 176 601 1.85e-50 181
Msa0251790 AT4G15870.1 27.934 426 291 5 32 453 178 591 3.06e-50 180
Msa0251790 AT3G29110.1 29.577 426 296 4 32 453 165 590 1.62e-49 178
Msa0251790 AT1G31950.2 30.145 345 224 6 31 364 171 509 9.17e-47 169
Msa0251790 AT4G20230.2 27.506 429 304 6 32 453 182 610 1.47e-45 168
Msa0251790 AT4G20230.1 27.506 429 304 6 32 453 170 598 1.57e-45 167
Msa0251790 AT4G20200.1 27.397 438 308 7 28 460 172 604 2.38e-44 164
Msa0251790 AT1G66020.1 28.506 435 295 8 36 460 170 598 5.32e-44 163
Msa0251790 AT5G44630.1 28.111 434 302 6 32 458 125 555 1.71e-43 161
Msa0251790 AT2G23230.2 27.962 422 298 4 37 453 173 593 7.48e-43 160
Msa0251790 AT4G20210.1 28.348 448 303 10 26 460 158 600 1.62e-42 159
Msa0251790 AT1G48820.1 29.524 420 273 8 42 455 157 559 2.45e-42 158
Msa0251790 AT3G14540.2 28.531 354 228 6 31 369 165 508 5.57e-42 156
Msa0251790 AT2G23230.1 28.029 421 294 4 37 453 173 588 1.05e-41 157
Msa0251790 AT1G48800.1 28.637 433 294 8 32 453 171 599 7.87e-41 154
Msa0251790 AT5G48110.1 28.363 342 239 6 121 460 2 339 2.69e-40 148
Msa0251790 AT4G13280.2 26.682 431 308 7 36 460 126 554 2.31e-38 147
Msa0251790 AT4G13300.1 26.402 428 309 6 36 458 126 552 6.42e-38 145
Msa0251790 AT1G79460.1 28.182 330 224 7 138 460 456 779 1.79e-34 137
Msa0251790 AT1G66020.2 32.922 243 153 6 32 269 164 401 7.23e-31 124
Msa0251790 AT4G13280.1 26.218 431 280 10 36 460 126 524 8.39e-31 125
Msa0251790 AT1G61120.1 29.885 348 210 9 60 389 379 710 2.60e-26 113
Msa0251790 AT4G02780.1 28.037 214 127 5 26 213 383 595 2.95e-12 69.3

Find 103 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTACTTGAATTTGCTAAAT+TGG 0.149491 2_2:-42103525 Msa0251790:CDS
ACCAGTTTAACAATGAAATT+AGG 0.174610 2_2:-42105062 Msa0251790:five_prime_UTR
GCAATTCGATTCTGAATTTC+AGG 0.188613 2_2:+42101853 None:intergenic
ATATGTTCTTATCTTCATTT+TGG 0.216264 2_2:+42103657 None:intergenic
TTAGCTATAAAAGGAAAATT+TGG 0.217455 2_2:+42106045 None:intergenic
CCCTAATTTCATTGTTAAAC+TGG 0.249670 2_2:+42105061 None:intergenic
CTAATTTCCAACCATCAATT+TGG 0.253018 2_2:-42105848 Msa0251790:five_prime_UTR
ATCATCAATGATGGTAATTA+AGG 0.253672 2_2:+42102854 None:intergenic
TTGTTTAGGTAATATATATT+AGG 0.277874 2_2:-42106128 Msa0251790:five_prime_UTR
GATTCCACATTCATTCAAAA+TGG 0.278555 2_2:-42102289 Msa0251790:CDS
TATACAAGTTATGCTTGTTT+AGG 0.291138 2_2:-42106142 None:intergenic
GTAAATCCATTAGCTATAAA+AGG 0.300976 2_2:+42106036 None:intergenic
TTGAGTTGTGCGATGGAAAT+TGG 0.303929 2_2:+42105929 None:intergenic
AAGAAAACTCCAACATAATT+AGG 0.306044 2_2:-42102248 Msa0251790:CDS
TTTCGTAAATATTTAAAATC+TGG 0.309886 2_2:+42102897 None:intergenic
ATCATTAAGTAGTGAATATA+AGG 0.309945 2_2:-42105808 Msa0251790:intron
TGCGACTTAAATGATAGTTT+TGG 0.311392 2_2:+42104901 None:intergenic
CCAGTTTAACAATGAAATTA+GGG 0.313400 2_2:-42105061 Msa0251790:five_prime_UTR
CAATGAAATTAGGGACATAT+TGG 0.313580 2_2:-42105052 Msa0251790:five_prime_UTR
ACGTATACTTAAGGTCTTCT+TGG 0.327820 2_2:+42103477 None:intergenic
TCTCTTGCTTCATCTAATAT+AGG 0.328498 2_2:+42103713 None:intergenic
TCAATGCAAGGTATCATATA+AGG 0.340753 2_2:-42105910 Msa0251790:five_prime_UTR
CAATGCTACATGAATGAAAC+TGG 0.341142 2_2:-42102054 Msa0251790:CDS
AAGTATAGTAGGTGCACTTA+TGG 0.345706 2_2:+42102329 None:intergenic
GATTCTGAATTTCAGGATCT+TGG 0.349688 2_2:+42101860 None:intergenic
ATTTGGTTTGTTCTCTTCGT+TGG 0.357582 2_2:+42106062 None:intergenic
CTAATGGATTTACCTATGAT+TGG 0.377736 2_2:-42106026 Msa0251790:five_prime_UTR
TTTGTACATACAATAGGAAT+AGG 0.380424 2_2:+42101814 None:intergenic
GAGCTTTGCAAGGGATAGAT+TGG 0.394984 2_2:-42102958 Msa0251790:CDS
ACTTCAAATAAGTCATGCTT+TGG 0.406823 2_2:-42103633 Msa0251790:CDS
TTTGGAGCATCCACTACATT+GGG 0.413445 2_2:-42103615 Msa0251790:CDS
TAAGCATGAGTAAGTATAGT+AGG 0.415253 2_2:+42102318 None:intergenic
CTAGAAAGAGTTCCAATCAT+AGG 0.420371 2_2:+42106014 None:intergenic
TTTGGTTTGTTCTCTTCGTT+GGG 0.422651 2_2:+42106063 None:intergenic
GATTGGAACTCTTTCTAGCT+TGG 0.425228 2_2:-42106009 Msa0251790:five_prime_UTR
GCTTCCTCCTGTTGAAAAGA+AGG 0.427249 2_2:-42105976 Msa0251790:five_prime_UTR
TTTCAGGATCTTGGTTGGTA+TGG 0.431471 2_2:+42101869 None:intergenic
TGCTGTAAATCTTGCTAGAA+TGG 0.435206 2_2:-42101920 Msa0251790:CDS
TGATCATAGGTCCAAATTGA+TGG 0.438502 2_2:+42105837 None:intergenic
ATGTAGCATTGAATTGACTT+TGG 0.445177 2_2:+42102066 None:intergenic
AGCACCTTGACGTATACTTA+AGG 0.445348 2_2:+42103468 None:intergenic
TAATGATTGTATATGATCAT+AGG 0.449975 2_2:+42105824 None:intergenic
TGCTGAAAGCCTAATTATGT+TGG 0.450526 2_2:+42102239 None:intergenic
TCTTCACAAAAGCGGTTGAT+AGG 0.463755 2_2:-42102794 Msa0251790:intron
AGGGTTCTACTGTAGCAATA+TGG 0.469057 2_2:-42105956 Msa0251790:five_prime_UTR
GGCTTGGTGTGTATCCACTA+TGG 0.480255 2_2:+42102382 None:intergenic
CTGAATTTCAGGATCTTGGT+TGG 0.483854 2_2:+42101864 None:intergenic
ATTGATGTATTGCAAAGGCT+TGG 0.488943 2_2:-42105093 Msa0251790:five_prime_UTR
CATGGTTATGATATATCTAC+AGG 0.496838 2_2:-42104949 Msa0251790:CDS
TTCATTCAAAATGGAAGACT+TGG 0.497580 2_2:-42102280 Msa0251790:CDS
CTTTGGAGCATCCACTACAT+TGG 0.499360 2_2:-42103616 Msa0251790:CDS
GAAATATATAACTTCAAGAA+AGG 0.507728 2_2:-42103803 Msa0251790:CDS
TTTGACAACAATGGTCGTAA+AGG 0.510240 2_2:+42104859 None:intergenic
AAAGCAATGCTCAATTCTAA+AGG 0.512815 2_2:-42105172 Msa0251790:five_prime_UTR
TCTAAGTATTCTCCAAGGCT+TGG 0.517090 2_2:+42102366 None:intergenic
CTTCCTCCTGTTGAAAAGAA+GGG 0.519768 2_2:-42105975 Msa0251790:five_prime_UTR
GAATTCCTCGATGGGAGGCT+AGG 0.520655 2_2:-42103592 Msa0251790:CDS
TTTATGTATCATCGTGGAGA+TGG 0.524795 2_2:-42101892 Msa0251790:CDS
TGGCTTGAAATTTGTTCCCA+CGG 0.528309 2_2:+42102917 None:intergenic
AGGTCGGTTCATTGATGTTG+AGG 0.530302 2_2:-42103783 Msa0251790:CDS
AGTGGATACACACCAAGCCT+TGG 0.533055 2_2:-42102378 Msa0251790:CDS
CATATTTGATTGAAGCAAAG+TGG 0.534124 2_2:-42102407 Msa0251790:CDS
GAGAATACTTAGAGAATGCA+TGG 0.536552 2_2:-42102356 Msa0251790:CDS
ATCTTCAAACGAAAGAAATG+AGG 0.538104 2_2:+42103739 None:intergenic
GAGATAATTTGAGCTTTGCA+AGG 0.540649 2_2:-42102968 Msa0251790:CDS
AACTGGAGCTTCTGAAGTTG+AGG 0.542366 2_2:-42102037 Msa0251790:CDS
AAACATGTTTATTAGTTCAG+TGG 0.543270 2_2:-42101747 Msa0251790:three_prime_UTR
TATATAACTTCAAGAAAGGT+CGG 0.544349 2_2:-42103799 Msa0251790:CDS
GGTCGGTTCATTGATGTTGA+GGG 0.544602 2_2:-42103782 Msa0251790:CDS
CTTCCACTCTATAAACATCA+TGG 0.551044 2_2:+42101996 None:intergenic
ATCGTACACATCATCAATGA+TGG 0.554157 2_2:+42102845 None:intergenic
CATAGGTCCAAATTGATGGT+TGG 0.559161 2_2:+42105841 None:intergenic
TTACGCCTTGCTAATGACCT+CGG 0.563093 2_2:-42102210 Msa0251790:CDS
GATGATGTGTACGATGTGTA+TGG 0.566503 2_2:-42102837 Msa0251790:CDS
AGATAATTTGAGCTTTGCAA+GGG 0.566504 2_2:-42102967 Msa0251790:CDS
AACTTACTTTATATGTTCCG+AGG 0.571077 2_2:+42102193 None:intergenic
TTCAGACTCTTGAGGCAACA+TGG 0.576372 2_2:-42104967 Msa0251790:exon
AGTCCATGATGTTTATAGAG+TGG 0.576983 2_2:-42101999 Msa0251790:CDS
CGAGGAATTCCCCAATGTAG+TGG 0.578520 2_2:+42103605 None:intergenic
TAGATGGGATCTAAATGCCA+TGG 0.583752 2_2:-42102672 Msa0251790:CDS
TACATTGGGGAATTCCTCGA+TGG 0.586748 2_2:-42103601 Msa0251790:CDS
GTAGTTTGGGAGAGAATCCA+TGG 0.590044 2_2:+42102655 None:intergenic
CCTTGCATTGAGTTGTGCGA+TGG 0.594872 2_2:+42105922 None:intergenic
AGTTTATTGATGTATTGCAA+AGG 0.603533 2_2:-42105098 Msa0251790:five_prime_UTR
ATGTTCCGAGGTCATTAGCA+AGG 0.610811 2_2:+42102205 None:intergenic
CCATCGCACAACTCAATGCA+AGG 0.612454 2_2:-42105922 Msa0251790:five_prime_UTR
TTTACGAAAAGTTATGACAA+AGG 0.613165 2_2:-42102883 Msa0251790:CDS
GAAGCAAAGTGGTACCATAG+TGG 0.615723 2_2:-42102396 Msa0251790:CDS
TTCCTCGATGGGAGGCTAGG+TGG 0.625455 2_2:-42103589 Msa0251790:CDS
TGGTGGAAAAGAACTAAACT+CGG 0.628476 2_2:-42102990 Msa0251790:CDS
ACTAGAGCTCTTCACAAAAG+CGG 0.638851 2_2:-42102802 Msa0251790:CDS
ACATTGGGGAATTCCTCGAT+GGG 0.641840 2_2:-42103600 Msa0251790:CDS
GAGTCTGAACTCAAGTGCTG+TGG 0.642243 2_2:+42104981 None:intergenic
CATTCTCTAAGTATTCTCCA+AGG 0.643831 2_2:+42102361 None:intergenic
TTGGGGAATTCCTCGATGGG+AGG 0.647931 2_2:-42103597 Msa0251790:CDS
CACTTGAGTTCAGACTCTTG+AGG 0.649831 2_2:-42104975 Msa0251790:five_prime_UTR
AAACAATCAATACACTAACA+AGG 0.650702 2_2:+42101775 None:intergenic
GGTTCATTGATATATACGAA+AGG 0.664912 2_2:-42103568 Msa0251790:CDS
TTGGAGCATCCACTACATTG+GGG 0.673224 2_2:-42103614 Msa0251790:CDS
GCATTGTTTATGTATCATCG+TGG 0.679800 2_2:-42101898 Msa0251790:CDS
AAGACCTTAAGTATACGTCA+AGG 0.685708 2_2:-42103472 Msa0251790:intron
GTACGATGTGTATGGCACAT+TGG 0.688383 2_2:-42102829 Msa0251790:CDS
AACCACCTAGCCTCCCATCG+AGG 0.722077 2_2:+42103587 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTACATTAAAAAAATTCATA+TGG - chr2_2:42102327-42102346 Msa0251790:CDS 10.0%
!!! TAAAAAAATCTTTTCAAAAA+TGG - chr2_2:42102413-42102432 Msa0251790:CDS 10.0%
!! AGTAAAAAAAAATTGATAAA+TGG + chr2_2:42102571-42102590 None:intergenic 10.0%
!!! ATTTAACATTATTTTTGTTT+TGG - chr2_2:42103748-42103767 Msa0251790:CDS 10.0%
!! ATAATATTCTTAGTAATATA+TGG + chr2_2:42103968-42103987 None:intergenic 10.0%
!! TTGTTTAGGTAATATATATT+AGG - chr2_2:42101732-42101751 Msa0251790:three_prime_UTR 15.0%
!! CAATCTTTCAAAAATATATT+AGG - chr2_2:42101762-42101781 Msa0251790:three_prime_UTR 15.0%
!!! AATTTTGAGATAATTTTCTT+AGG + chr2_2:42102253-42102272 None:intergenic 15.0%
!!! TTAAGAAATTATTTTTGTGT+GGG - chr2_2:42102363-42102382 Msa0251790:CDS 15.0%
!!! ATGAAAAACAAAATTTTGTA+GGG - chr2_2:42102529-42102548 Msa0251790:intron 15.0%
!! AAAATTGCAATTAAAATCAA+AGG + chr2_2:42103842-42103861 None:intergenic 15.0%
!!! TAATATATGGTTTCAAATTT+TGG + chr2_2:42103955-42103974 None:intergenic 15.0%
!!! TTTCGTAAATATTTAAAATC+TGG + chr2_2:42104966-42104985 None:intergenic 15.0%
!! GAAAATATTTCAATAAATTG+AGG + chr2_2:42105127-42105146 None:intergenic 15.0%
!!! TGTTATTGTTATTATTATAG+TGG - chr2_2:42105414-42105433 Msa0251790:intron 15.0%
!! TTAGCTATAAAAGGAAAATT+TGG + chr2_2:42101818-42101837 None:intergenic 20.0%
!!! AATTTTCCTTTTATAGCTAA+TGG - chr2_2:42101818-42101837 Msa0251790:CDS 20.0%
!! TAATGATTGTATATGATCAT+AGG + chr2_2:42102039-42102058 None:intergenic 20.0%
!! ATCATTAAGTAGTGAATATA+AGG - chr2_2:42102052-42102071 Msa0251790:CDS 20.0%
!!! GCATAATAATTTTTTAGAAC+TGG + chr2_2:42102144-42102163 None:intergenic 20.0%
!!! CTTAAGAAATTATTTTTGTG+TGG - chr2_2:42102362-42102381 Msa0251790:CDS 20.0%
!!! TAAGAAATTATTTTTGTGTG+GGG - chr2_2:42102364-42102383 Msa0251790:CDS 20.0%
!!! AAAATCTTTTCAAAAATGGA+AGG - chr2_2:42102417-42102436 Msa0251790:CDS 20.0%
!!! TAAACATGATTTGTTTCAAT+AGG - chr2_2:42102445-42102464 Msa0251790:intron 20.0%
!!! AAACATGATTTGTTTCAATA+GGG - chr2_2:42102446-42102465 Msa0251790:intron 20.0%
!!! AAAGTGTTATTCTTTTTATG+TGG - chr2_2:42102506-42102525 Msa0251790:intron 20.0%
!!! GATGAAAAACAAAATTTTGT+AGG - chr2_2:42102528-42102547 Msa0251790:intron 20.0%
!!! TGTTAGCATTTTTCATATTT+AGG + chr2_2:42102637-42102656 None:intergenic 20.0%
!!! TTTGCTTTAAGTATTTTGTT+AGG - chr2_2:42103515-42103534 Msa0251790:CDS 20.0%
!! TATGACTACTAAAGAATATT+TGG - chr2_2:42103650-42103669 Msa0251790:CDS 20.0%
!! GAAATATATAACTTCAAGAA+AGG - chr2_2:42104057-42104076 Msa0251790:intron 20.0%
!!! ATATGTTCTTATCTTCATTT+TGG + chr2_2:42104206-42104225 None:intergenic 20.0%
!!! TTTACTTGAATTTGCTAAAT+TGG - chr2_2:42104335-42104354 Msa0251790:intron 20.0%
!! TAGAAACTAATACTTGAAAT+CGG + chr2_2:42104420-42104439 None:intergenic 20.0%
!!! TTTCATCAGAATTTAAGTTT+TGG - chr2_2:42104543-42104562 Msa0251790:intron 20.0%
!!! TTCATCAGAATTTAAGTTTT+GGG - chr2_2:42104544-42104563 Msa0251790:intron 20.0%
!! AAAAAAAGTTAAGCATTAGT+CGG + chr2_2:42104834-42104853 None:intergenic 20.0%
!!! ACTAATGCTTAACTTTTTTT+TGG - chr2_2:42104834-42104853 Msa0251790:intron 20.0%
!!! TTTTTGGTTACATTTTAAGA+TGG - chr2_2:42104850-42104869 Msa0251790:intron 20.0%
!! AACAATTAGCGAAAAATTTA+AGG + chr2_2:42105731-42105750 None:intergenic 20.0%
!!! TAATGTTTTGTACATACAAT+AGG + chr2_2:42106055-42106074 None:intergenic 20.0%
! GTAAATCCATTAGCTATAAA+AGG + chr2_2:42101827-42101846 None:intergenic 25.0%
!! TTCTTTGCACTTTTATCTTA+GGG - chr2_2:42102103-42102122 Msa0251790:intron 25.0%
!!! TTTGAGATAATTTTCTTAGG+AGG + chr2_2:42102250-42102269 None:intergenic 25.0%
! AGATAACAGTAACAAAAATG+TGG + chr2_2:42102299-42102318 None:intergenic 25.0%
!! TTCATATTTAGGACTTGTAA+TGG + chr2_2:42102626-42102645 None:intergenic 25.0%
! TATTTCCTGAATTAAGTAGA+AGG + chr2_2:42102660-42102679 None:intergenic 25.0%
! AGTTTATTGATGTATTGCAA+AGG - chr2_2:42102762-42102781 Msa0251790:intron 25.0%
! ACCAGTTTAACAATGAAATT+AGG - chr2_2:42102798-42102817 Msa0251790:CDS 25.0%
! CCAGTTTAACAATGAAATTA+GGG - chr2_2:42102799-42102818 Msa0251790:CDS 25.0%
!! TAAAATCCTGTGATTTTGAA+CGG + chr2_2:42103307-42103326 None:intergenic 25.0%
!! TCAAAATCACAGGATTTTAA+AGG - chr2_2:42103308-42103327 Msa0251790:intron 25.0%
!! ATGAATGTGTTGATATGATT+GGG - chr2_2:42103441-42103460 Msa0251790:intron 25.0%
! GTGATTTATGATTGCATATA+GGG - chr2_2:42103492-42103511 Msa0251790:CDS 25.0%
!! AAGTATTTTGTCTAACATCT+AGG - chr2_2:42103587-42103606 Msa0251790:CDS 25.0%
!!! TGCCTTTAAGTATTTTGTTT+CGG - chr2_2:42103613-42103632 Msa0251790:CDS 25.0%
! AACCGAAACAAAATACTTAA+AGG + chr2_2:42103618-42103637 None:intergenic 25.0%
! TATATAACTTCAAGAAAGGT+CGG - chr2_2:42104061-42104080 Msa0251790:intron 25.0%
!! CAAAAATTCTTTGAGAAACT+TGG + chr2_2:42104181-42104200 None:intergenic 25.0%
!! CAAGTTTCTCAAAGAATTTT+TGG - chr2_2:42104179-42104198 Msa0251790:intron 25.0%
!! GTTTCTAGATAAATATTGCA+TGG - chr2_2:42104433-42104452 Msa0251790:intron 25.0%
!!! TAACTTAGTTGCTCATTTTT+AGG - chr2_2:42104777-42104796 Msa0251790:intron 25.0%
! TTTACGAAAAGTTATGACAA+AGG - chr2_2:42104977-42104996 Msa0251790:five_prime_UTR 25.0%
! ATCATCAATGATGGTAATTA+AGG + chr2_2:42105009-42105028 None:intergenic 25.0%
!! ATTTTTCTTCTGATTCTAGA+TGG - chr2_2:42105172-42105191 Msa0251790:five_prime_UTR 25.0%
!! TTTTTCTTCTGATTCTAGAT+GGG - chr2_2:42105173-42105192 Msa0251790:five_prime_UTR 25.0%
!! AGCATATTTTCATGTAGTTT+GGG + chr2_2:42105221-42105240 None:intergenic 25.0%
!! AAGCATATTTTCATGTAGTT+TGG + chr2_2:42105222-42105241 None:intergenic 25.0%
!! TTTGAAACTTTGAAAAAGTG+TGG - chr2_2:42105276-42105295 Msa0251790:intron 25.0%
! AAGAAAACTCCAACATAATT+AGG - chr2_2:42105612-42105631 Msa0251790:intron 25.0%
!!! GTTTTTTTTGCTTCTCTAAA+CGG - chr2_2:42105687-42105706 Msa0251790:intron 25.0%
! TTTGTACATACAATAGGAAT+AGG + chr2_2:42106049-42106068 None:intergenic 25.0%
! AAACAATCAATACACTAACA+AGG + chr2_2:42106088-42106107 None:intergenic 25.0%
! AAACATGTTTATTAGTTCAG+TGG - chr2_2:42106113-42106132 Msa0251790:five_prime_UTR 25.0%
!! CTAATGGATTTACCTATGAT+TGG - chr2_2:42101834-42101853 Msa0251790:CDS 30.0%
! TCAATGCAAGGTATCATATA+AGG - chr2_2:42101950-42101969 Msa0251790:CDS 30.0%
CTAATTTCCAACCATCAATT+TGG - chr2_2:42102012-42102031 Msa0251790:CDS 30.0%
! CTTCTTTGCACTTTTATCTT+AGG - chr2_2:42102102-42102121 Msa0251790:intron 30.0%
TAGGGATTAACAATGTTTGA+AGG - chr2_2:42102464-42102483 Msa0251790:intron 30.0%
TAACACCTTCTACTTAATTC+AGG - chr2_2:42102652-42102671 Msa0251790:CDS 30.0%
AAAGCAATGCTCAATTCTAA+AGG - chr2_2:42102688-42102707 Msa0251790:intron 30.0%
! AAGTTGAACAAGCTCATTTT+CGG + chr2_2:42102741-42102760 None:intergenic 30.0%
CCCTAATTTCATTGTTAAAC+TGG + chr2_2:42102802-42102821 None:intergenic 30.0%
CAATGAAATTAGGGACATAT+TGG - chr2_2:42102808-42102827 Msa0251790:CDS 30.0%
CATGGTTATGATATATCTAC+AGG - chr2_2:42102911-42102930 Msa0251790:CDS 30.0%
! TGCGACTTAAATGATAGTTT+TGG + chr2_2:42102962-42102981 None:intergenic 30.0%
! ATAGGATTTTGTCTGTTTGT+GGG + chr2_2:42103260-42103279 None:intergenic 30.0%
! GATGAATGTGTTGATATGAT+TGG - chr2_2:42103440-42103459 Msa0251790:intron 30.0%
! TGAATGTGTTGATATGATTG+GGG - chr2_2:42103442-42103461 Msa0251790:intron 30.0%
TTGGGGATAATCCATATAAT+TGG - chr2_2:42103459-42103478 Msa0251790:intron 30.0%
CGTGATTTATGATTGCATAT+AGG - chr2_2:42103491-42103510 Msa0251790:CDS 30.0%
TAAGTGACTATGAAGTTTCA+AGG + chr2_2:42103554-42103573 None:intergenic 30.0%
CTAAAGAATATTTGGCATCT+AGG - chr2_2:42103658-42103677 Msa0251790:CDS 30.0%
! GACTTTTGTAGAATCGATAT+AGG + chr2_2:42103807-42103826 None:intergenic 30.0%
TAATCAAGGTTGTCAAAATC+GGG + chr2_2:42103908-42103927 None:intergenic 30.0%
GTAATCAAGGTTGTCAAAAT+CGG + chr2_2:42103909-42103928 None:intergenic 30.0%
!! GATTTTGACAACCTTGATTA+CGG - chr2_2:42103908-42103927 Msa0251790:intron 30.0%
ATCTTCAAACGAAAGAAATG+AGG + chr2_2:42104124-42104143 None:intergenic 30.0%
TCTCTTGCTTCATCTAATAT+AGG + chr2_2:42104150-42104169 None:intergenic 30.0%
ACTTCAAATAAGTCATGCTT+TGG - chr2_2:42104227-42104246 Msa0251790:intron 30.0%
GGTTCATTGATATATACGAA+AGG - chr2_2:42104292-42104311 Msa0251790:intron 30.0%
!! CTCTAAAACACGATAACAAA+TGG - chr2_2:42104629-42104648 Msa0251790:intron 30.0%
TGGAAAAACATTGCAATAGT+TGG - chr2_2:42104649-42104668 Msa0251790:intron 30.0%
!!! TTGGTTACATTTTAAGATGG+TGG - chr2_2:42104853-42104872 Msa0251790:intron 30.0%
AGATAATTTGAGCTTTGCAA+GGG - chr2_2:42104893-42104912 Msa0251790:CDS 30.0%
!! GATTGGTTGAGAATTTTGTT+TGG - chr2_2:42104919-42104938 Msa0251790:CDS 30.0%
!!! TTTTCAAGTATGGAGTGATA+TGG + chr2_2:42105305-42105324 None:intergenic 30.0%
!!! CTTACCACTTTTTTCAAGTA+TGG + chr2_2:42105315-42105334 None:intergenic 30.0%
CATATTTGATTGAAGCAAAG+TGG - chr2_2:42105453-42105472 Msa0251790:intron 30.0%
TAAGCATGAGTAAGTATAGT+AGG + chr2_2:42105545-42105564 None:intergenic 30.0%
GATTCCACATTCATTCAAAA+TGG - chr2_2:42105571-42105590 Msa0251790:intron 30.0%
! TCTTCCATTTTGAATGAATG+TGG + chr2_2:42105578-42105597 None:intergenic 30.0%
TTCATTCAAAATGGAAGACT+TGG - chr2_2:42105580-42105599 Msa0251790:intron 30.0%
AACTTACTTTATATGTTCCG+AGG + chr2_2:42105670-42105689 None:intergenic 30.0%
!!! CTCTTTTTCACGCTTTAAAA+AGG + chr2_2:42105762-42105781 None:intergenic 30.0%
! TTTAAAGCGTGAAAAAGAGA+CGG - chr2_2:42105763-42105782 Msa0251790:intron 30.0%
! ATGTAGCATTGAATTGACTT+TGG + chr2_2:42105797-42105816 None:intergenic 30.0%
!! TTTGGTTTGTTCTCTTCGTT+GGG + chr2_2:42101800-42101819 None:intergenic 35.0%
!! ATTTGGTTTGTTCTCTTCGT+TGG + chr2_2:42101801-42101820 None:intergenic 35.0%
CTAGAAAGAGTTCCAATCAT+AGG + chr2_2:42101849-42101868 None:intergenic 35.0%
!!! AACAGGAGGAAGCATTTTTT+TGG + chr2_2:42101877-42101896 None:intergenic 35.0%
TGATCATAGGTCCAAATTGA+TGG + chr2_2:42102026-42102045 None:intergenic 35.0%
ATTGATGTATTGCAAAGGCT+TGG - chr2_2:42102767-42102786 Msa0251790:intron 35.0%
TTTGACAACAATGGTCGTAA+AGG + chr2_2:42103004-42103023 None:intergenic 35.0%
!! AGTCACGATTTTGACAACAA+TGG + chr2_2:42103013-42103032 None:intergenic 35.0%
TGACTTAAGTCTCAAAATCC+AGG - chr2_2:42103028-42103047 Msa0251790:intron 35.0%
CTAAGTAAAGTTGCAAATCC+TGG + chr2_2:42103049-42103068 None:intergenic 35.0%
TCAAATTCATAAACCCGCTT+GGG - chr2_2:42103079-42103098 Msa0251790:intron 35.0%
! GATAGGATTTTGTCTGTTTG+TGG + chr2_2:42103261-42103280 None:intergenic 35.0%
!! CGATTTTGTGCGATTTTGAT+AGG + chr2_2:42103278-42103297 None:intergenic 35.0%
TGAAATCCGTTCAAAATCAC+AGG - chr2_2:42103298-42103317 Msa0251790:intron 35.0%
!! ACATGACTCGGTTCTATAAT+GGG - chr2_2:42103396-42103415 Msa0251790:intron 35.0%
CGAAGAAATCGCCAATTATA+TGG + chr2_2:42103473-42103492 None:intergenic 35.0%
! GATTTTACTTCAGGTCGAAA+GGG + chr2_2:42103886-42103905 None:intergenic 35.0%
! CAAAATCGGGATTTTACTTC+AGG + chr2_2:42103895-42103914 None:intergenic 35.0%
ACGTATACTTAAGGTCTTCT+TGG + chr2_2:42104386-42104405 None:intergenic 35.0%
AAGACCTTAAGTATACGTCA+AGG - chr2_2:42104388-42104407 Msa0251790:intron 35.0%
TGGTGGAAAAGAACTAAACT+CGG - chr2_2:42104870-42104889 Msa0251790:CDS 35.0%
GAGATAATTTGAGCTTTGCA+AGG - chr2_2:42104892-42104911 Msa0251790:CDS 35.0%
ATCGTACACATCATCAATGA+TGG + chr2_2:42105018-42105037 None:intergenic 35.0%
CACTCCATACTTGAAAAAAG+TGG - chr2_2:42105308-42105327 Msa0251790:intron 35.0%
CATTCTCTAAGTATTCTCCA+AGG + chr2_2:42105502-42105521 None:intergenic 35.0%
GAGAATACTTAGAGAATGCA+TGG - chr2_2:42105504-42105523 Msa0251790:intron 35.0%
AAGTATAGTAGGTGCACTTA+TGG + chr2_2:42105534-42105553 None:intergenic 35.0%
TGCTGAAAGCCTAATTATGT+TGG + chr2_2:42105624-42105643 None:intergenic 35.0%
TTAAAGCGTGAAAAAGAGAC+GGG - chr2_2:42105764-42105783 Msa0251790:intron 35.0%
CAATGCTACATGAATGAAAC+TGG - chr2_2:42105806-42105825 Msa0251790:intron 35.0%
AGTCCATGATGTTTATAGAG+TGG - chr2_2:42105861-42105880 Msa0251790:five_prime_UTR 35.0%
CTTCCACTCTATAAACATCA+TGG + chr2_2:42105867-42105886 None:intergenic 35.0%
TGCTGTAAATCTTGCTAGAA+TGG - chr2_2:42105940-42105959 Msa0251790:five_prime_UTR 35.0%
GCATTGTTTATGTATCATCG+TGG - chr2_2:42105962-42105981 Msa0251790:five_prime_UTR 35.0%
TTTATGTATCATCGTGGAGA+TGG - chr2_2:42105968-42105987 Msa0251790:five_prime_UTR 35.0%
GATTCTGAATTTCAGGATCT+TGG + chr2_2:42106003-42106022 None:intergenic 35.0%
GCAATTCGATTCTGAATTTC+AGG + chr2_2:42106010-42106029 None:intergenic 35.0%
!! GATTGGAACTCTTTCTAGCT+TGG - chr2_2:42101851-42101870 Msa0251790:CDS 40.0%
CTTCCTCCTGTTGAAAAGAA+GGG - chr2_2:42101885-42101904 Msa0251790:CDS 40.0%
! GTAGAACCCTTCTTTTCAAC+AGG + chr2_2:42101894-42101913 None:intergenic 40.0%
AGGGTTCTACTGTAGCAATA+TGG - chr2_2:42101904-42101923 Msa0251790:CDS 40.0%
! TTGAGTTGTGCGATGGAAAT+TGG + chr2_2:42101934-42101953 None:intergenic 40.0%
! CATAGGTCCAAATTGATGGT+TGG + chr2_2:42102022-42102041 None:intergenic 40.0%
CTCAAATTCATAAACCCGCT+TGG - chr2_2:42103078-42103097 Msa0251790:intron 40.0%
! ATTTCCTCTGGTGCCAATTT+AGG - chr2_2:42103163-42103182 Msa0251790:intron 40.0%
CTAAACTAGCCCACCTAAAT+TGG + chr2_2:42103179-42103198 None:intergenic 40.0%
CATAGTAGAATCCCACAAAC+AGG - chr2_2:42103225-42103244 Msa0251790:intron 40.0%
ACCGAGTCATGTGTACAATT+CGG + chr2_2:42103388-42103407 None:intergenic 40.0%
!! CACATGACTCGGTTCTATAA+TGG - chr2_2:42103395-42103414 Msa0251790:intron 40.0%
!! CATGACTCGGTTCTATAATG+GGG - chr2_2:42103397-42103416 Msa0251790:intron 40.0%
!! ATGACTCGGTTCTATAATGG+GGG - chr2_2:42103398-42103417 Msa0251790:intron 40.0%
! GGATTTTACTTCAGGTCGAA+AGG + chr2_2:42103887-42103906 None:intergenic 40.0%
! TTTGGAGCATCCACTACATT+GGG - chr2_2:42104245-42104264 Msa0251790:intron 40.0%
AGCACCTTGACGTATACTTA+AGG + chr2_2:42104395-42104414 None:intergenic 40.0%
!! TGAGAATTTTGTTTGGACCG+TGG - chr2_2:42104926-42104945 Msa0251790:CDS 40.0%
!! GAGAATTTTGTTTGGACCGT+GGG - chr2_2:42104927-42104946 Msa0251790:CDS 40.0%
! TGGCTTGAAATTTGTTCCCA+CGG + chr2_2:42104946-42104965 None:intergenic 40.0%
GATGATGTGTACGATGTGTA+TGG - chr2_2:42105023-42105042 Msa0251790:five_prime_UTR 40.0%
ACTAGAGCTCTTCACAAAAG+CGG - chr2_2:42105058-42105077 Msa0251790:five_prime_UTR 40.0%
TCTTCACAAAAGCGGTTGAT+AGG - chr2_2:42105066-42105085 Msa0251790:five_prime_UTR 40.0%
TAGATGGGATCTAAATGCCA+TGG - chr2_2:42105188-42105207 Msa0251790:five_prime_UTR 40.0%
TCTAAGTATTCTCCAAGGCT+TGG + chr2_2:42105497-42105516 None:intergenic 40.0%
! TTTCAGGATCTTGGTTGGTA+TGG + chr2_2:42105994-42106013 None:intergenic 40.0%
CTGAATTTCAGGATCTTGGT+TGG + chr2_2:42105999-42106018 None:intergenic 40.0%
GCTTCCTCCTGTTGAAAAGA+AGG - chr2_2:42101884-42101903 Msa0251790:CDS 45.0%
! GAACCCTTCTTTTCAACAGG+AGG + chr2_2:42101891-42101910 None:intergenic 45.0%
CACTTGAGTTCAGACTCTTG+AGG - chr2_2:42102885-42102904 Msa0251790:CDS 45.0%
TTCAGACTCTTGAGGCAACA+TGG - chr2_2:42102893-42102912 Msa0251790:CDS 45.0%
ATTCATAAACCCGCTTGGGT+TGG - chr2_2:42103083-42103102 Msa0251790:intron 45.0%
TTGGCCTAGTGGTATTAGCT+TGG - chr2_2:42103102-42103121 Msa0251790:intron 45.0%
! TGGCCTAGTGGTATTAGCTT+GGG - chr2_2:42103103-42103122 Msa0251790:intron 45.0%
! GGAGGATTTTGCCTGTTTGT+GGG + chr2_2:42103239-42103258 None:intergenic 45.0%
! GGATTTTGTCTGTTTGTGGG+AGG + chr2_2:42103257-42103276 None:intergenic 45.0%
TTCGGGTCATTTGACGAACT+GGG + chr2_2:42103370-42103389 None:intergenic 45.0%
ATTCGGGTCATTTGACGAAC+TGG + chr2_2:42103371-42103390 None:intergenic 45.0%
CCGAGTCATGTGTACAATTC+GGG + chr2_2:42103387-42103406 None:intergenic 45.0%
CCCGAATTGTACACATGACT+CGG - chr2_2:42103384-42103403 Msa0251790:intron 45.0%
CTCAATCACAGCCGTAATCA+AGG + chr2_2:42103922-42103941 None:intergenic 45.0%
CTTGATTACGGCTGTGATTG+AGG - chr2_2:42103920-42103939 Msa0251790:intron 45.0%
! AGGTCGGTTCATTGATGTTG+AGG - chr2_2:42104077-42104096 Msa0251790:intron 45.0%
! GGTCGGTTCATTGATGTTGA+GGG - chr2_2:42104078-42104097 Msa0251790:intron 45.0%
!! CTTTGGAGCATCCACTACAT+TGG - chr2_2:42104244-42104263 Msa0251790:intron 45.0%
! TTGGAGCATCCACTACATTG+GGG - chr2_2:42104246-42104265 Msa0251790:intron 45.0%
TACATTGGGGAATTCCTCGA+TGG - chr2_2:42104259-42104278 Msa0251790:intron 45.0%
ACATTGGGGAATTCCTCGAT+GGG - chr2_2:42104260-42104279 Msa0251790:intron 45.0%
GAGCTTTGCAAGGGATAGAT+TGG - chr2_2:42104902-42104921 Msa0251790:CDS 45.0%
GTACGATGTGTATGGCACAT+TGG - chr2_2:42105031-42105050 Msa0251790:five_prime_UTR 45.0%
GTAGTTTGGGAGAGAATCCA+TGG + chr2_2:42105208-42105227 None:intergenic 45.0%
GAAGCAAAGTGGTACCATAG+TGG - chr2_2:42105464-42105483 Msa0251790:intron 45.0%
TTACGCCTTGCTAATGACCT+CGG - chr2_2:42105650-42105669 Msa0251790:intron 45.0%
ATGTTCCGAGGTCATTAGCA+AGG + chr2_2:42105658-42105677 None:intergenic 45.0%
!! AACTGGAGCTTCTGAAGTTG+AGG - chr2_2:42105823-42105842 Msa0251790:five_prime_UTR 45.0%
!!! TTTTTTTCATTTTTTTTATA+TGG - chr2_2:42103332-42103351 Msa0251790:intron 5.0%
!!! TTTTTTCATTTTTTTTATAT+GGG - chr2_2:42103333-42103352 Msa0251790:intron 5.0%
!! AGATATATTATTAATTTAAA+TGG - chr2_2:42104682-42104701 Msa0251790:intron 5.0%
!!! AATTTTTTAAATAATAGATT+AGG + chr2_2:42104736-42104755 None:intergenic 5.0%
! CCTTGCATTGAGTTGTGCGA+TGG + chr2_2:42101941-42101960 None:intergenic 50.0%
CCATCGCACAACTCAATGCA+AGG - chr2_2:42101938-42101957 Msa0251790:CDS 50.0%
! GAGTCTGAACTCAAGTGCTG+TGG + chr2_2:42102882-42102901 None:intergenic 50.0%
GGTCCCAAGCTAATACCACT+AGG + chr2_2:42103109-42103128 None:intergenic 50.0%
! AGTGGTATTAGCTTGGGACC+TGG - chr2_2:42103109-42103128 Msa0251790:intron 50.0%
! GTGGTATTAGCTTGGGACCT+GGG - chr2_2:42103110-42103129 Msa0251790:intron 50.0%
AAATCGAACCTGAGACCTCG+AGG + chr2_2:42103147-42103166 None:intergenic 50.0%
GTCTCAGGTTCGATTTCCTC+TGG - chr2_2:42103151-42103170 Msa0251790:intron 50.0%
! TCCTCTGGTGCCAATTTAGG+TGG - chr2_2:42103166-42103185 Msa0251790:intron 50.0%
! CCTCTGGTGCCAATTTAGGT+GGG - chr2_2:42103167-42103186 Msa0251790:intron 50.0%
! GGGAGGATTTTGCCTGTTTG+TGG + chr2_2:42103240-42103259 None:intergenic 50.0%
CGAGGAATTCCCCAATGTAG+TGG + chr2_2:42104258-42104277 None:intergenic 50.0%
!! GGCTTGGTGTGTATCCACTA+TGG + chr2_2:42105481-42105500 None:intergenic 50.0%
AGTGGATACACACCAAGCCT+TGG - chr2_2:42105482-42105501 Msa0251790:intron 50.0%
TACCACTAGGCCAACCCAAG+CGG + chr2_2:42103096-42103115 None:intergenic 55.0%
CCCACCTAAATTGGCACCAG+AGG + chr2_2:42103170-42103189 None:intergenic 55.0%
TTGGGGAATTCCTCGATGGG+AGG - chr2_2:42104263-42104282 Msa0251790:intron 55.0%
GAATTCCTCGATGGGAGGCT+AGG - chr2_2:42104268-42104287 Msa0251790:intron 55.0%
ACCACTAGGCCAACCCAAGC+GGG + chr2_2:42103095-42103114 None:intergenic 60.0%
TCGAACCTGAGACCTCGAGG+AGG + chr2_2:42103144-42103163 None:intergenic 60.0%
TTCCTCGATGGGAGGCTAGG+TGG - chr2_2:42104271-42104290 Msa0251790:intron 60.0%
AACCACCTAGCCTCCCATCG+AGG + chr2_2:42104276-42104295 None:intergenic 60.0%
!! ACCCGCTTGGGTTGGCCTAG+TGG - chr2_2:42103091-42103110 Msa0251790:intron 65.0%
! TCGAGGAGGAGCACACTCCC+AGG + chr2_2:42103130-42103149 None:intergenic 65.0%
! TGGGAGTGTGCTCCTCCTCG+AGG - chr2_2:42103129-42103148 Msa0251790:intron 65.0%
GTGCTCCTCCTCGAGGTCTC+AGG - chr2_2:42103136-42103155 Msa0251790:intron 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 42101731 42106151 42101731 ID=Msa0251790;Name=Msa0251790
chr2_2 mRNA 42101731 42106151 42101731 ID=Msa0251790-mRNA-1;Parent=Msa0251790;Name=Msa0251790-mRNA-1;_AED=0.08;_eAED=0.08;_QI=581|1|1|1|0.83|0.71|7|76|465
chr2_2 exon 42101731 42102112 42101731 ID=Msa0251790-mRNA-1:exon:13162;Parent=Msa0251790-mRNA-1
chr2_2 exon 42102200 42102448 42102200 ID=Msa0251790-mRNA-1:exon:13161;Parent=Msa0251790-mRNA-1
chr2_2 exon 42102553 42102691 42102553 ID=Msa0251790-mRNA-1:exon:13160;Parent=Msa0251790-mRNA-1
chr2_2 exon 42102795 42103013 42102795 ID=Msa0251790-mRNA-1:exon:13159;Parent=Msa0251790-mRNA-1
chr2_2 exon 42103473 42103851 42103473 ID=Msa0251790-mRNA-1:exon:13158;Parent=Msa0251790-mRNA-1
chr2_2 exon 42104865 42105208 42104865 ID=Msa0251790-mRNA-1:exon:13157;Parent=Msa0251790-mRNA-1
chr2_2 exon 42105809 42106151 42105809 ID=Msa0251790-mRNA-1:exon:13156;Parent=Msa0251790-mRNA-1
chr2_2 five_prime_UTR 42105809 42106151 42105809 ID=Msa0251790-mRNA-1:five_prime_utr;Parent=Msa0251790-mRNA-1
chr2_2 five_prime_UTR 42104971 42105208 42104971 ID=Msa0251790-mRNA-1:five_prime_utr;Parent=Msa0251790-mRNA-1
chr2_2 CDS 42104865 42104970 42104865 ID=Msa0251790-mRNA-1:cds;Parent=Msa0251790-mRNA-1
chr2_2 CDS 42103473 42103851 42103473 ID=Msa0251790-mRNA-1:cds;Parent=Msa0251790-mRNA-1
chr2_2 CDS 42102795 42103013 42102795 ID=Msa0251790-mRNA-1:cds;Parent=Msa0251790-mRNA-1
chr2_2 CDS 42102553 42102691 42102553 ID=Msa0251790-mRNA-1:cds;Parent=Msa0251790-mRNA-1
chr2_2 CDS 42102200 42102448 42102200 ID=Msa0251790-mRNA-1:cds;Parent=Msa0251790-mRNA-1
chr2_2 CDS 42101807 42102112 42101807 ID=Msa0251790-mRNA-1:cds;Parent=Msa0251790-mRNA-1
chr2_2 three_prime_UTR 42101731 42101806 42101731 ID=Msa0251790-mRNA-1:three_prime_utr;Parent=Msa0251790-mRNA-1
Gene Sequence

>Msa0251790

ATGGTTATGATATATCTACAGGTCATTGAATCTATAAGAAAAAAGCTCCAAAACTATCATTTAAGTCGCAATATTATGATTTTTCATATCCTTTACGACCATTGTTCAGATATTTTTGTTTTCTTTGAAGATGAAATATATAACTTCAAGAAAGGTCGGTTCATTGATGTTGAGGGAATGTTATCATTGTATGAAGCCTCATTTCTTTCGTTTGAAGATGAACCTATATTAGATGAAGCAAGAGATGTCACATCCAAGTTTCTCAAAGAATTTTTGGACCAAAATGAAGATAAGAACATATCACTTCAAATAAGTCATGCTTTGGAGCATCCACTACATTGGGGAATTCCTCGATGGGAGGCTAGGTGGTTCATTGATATATACGAAAGGCAACAAAATAAGAATCATGTTTTACTTGAATTTGCTAAATTGGATTTCAATATTCTTCAAAGCTTATACCAAGAAGACCTTAAGTATACGTCAAGATGGTGGAAAAGAACTAAACTCGGAGATAATTTGAGCTTTGCAAGGGATAGATTGGTTGAGAATTTTGTTTGGACCGTGGGAACAAATTTCAAGCCAGATTTTAAATATTTACGAAAAGTTATGACAAAGGTCAATTCCTTAATTACCATCATTGATGATGTGTACGATGTGTATGGCACATTGGAAGAACTAGAGCTCTTCACAAAAGCGGTTGATAGATGGGATCTAAATGCCATGGATTCTCTCCCAAACTACATGAAAATATGCTTTCATGCACTCTATAACTTTGTGAGTGAACTTGCTTTTGAAACTTTGAAAAAGTGTGGCTACCATATCACTCCATACTTGAAAAAAGTGTGGACAGATCTATGTAAATCATATTTGATTGAAGCAAAGTGGTACCATAGTGGATACACACCAAGCCTTGGAGAATACTTAGAGAATGCATGGATATCCATAAGTGCACCTACTATACTTACTCATGCTTACTTTGTGATTCCACATTCATTCAAAATGGAAGACTTGGTTAGTTTAGAAGAAAACTCCAACATAATTAGGCTTTCAGCAATAATTTTACGCCTTGCTAATGACCTCGGAACATATAAACGTGAAAAAGAGACGGGTGATATTCCAAAGTCAATTCAATGCTACATGAATGAAACTGGAGCTTCTGAAGTTGAGGCTTGTGAGTATGTTAAGTCCATGATGTTTATAGAGTGGAAGAAGTTGAATAAAGAAGCTCATAGTTCATCTTTCTCTCAATATTTCATAGATACTGCTGTAAATCTTGCTAGAATGGCATTGTTTATGTATCATCGTGGAGATGGCCATACCAACCAAGATCCTGAAATTCAGAATCGAATTGCATCATTAGTTTTTCAGCCTATTCCTATTGTATGTACAAAACATTAA
Protein sequence

>Msa0251790

MVMIYLQVIESIRKKLQNYHLSRNIMIFHILYDHCSDIFVFFEDEIYNFKKGRFIDVEGMLSLYEASFLSFEDEPILDEARDVTSKFLKEFLDQNEDKNISLQISHALEHPLHWGIPRWEARWFIDIYERQQNKNHVLLEFAKLDFNILQSLYQEDLKYTSRWWKRTKLGDNLSFARDRLVENFVWTVGTNFKPDFKYLRKVMTKVNSLITIIDDVYDVYGTLEELELFTKAVDRWDLNAMDSLPNYMKICFHALYNFVSELAFETLKKCGYHITPYLKKVWTDLCKSYLIEAKWYHSGYTPSLGEYLENAWISISAPTILTHAYFVIPHSFKMEDLVSLEENSNIIRLSAIILRLANDLGTYKREKETGDIPKSIQCYMNETGASEVEACEYVKSMMFIEWKKLNKEAHSSSFSQYFIDTAVNLARMALFMYHRGDGHTNQDPEIQNRIASLVFQPIPIVCTKH*