AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0753790


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0753790 MtrunA17_Chr8g0375221 91.667 36 3 0 35 70 317 352 9.96e-17 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0753790 AT2G02148.2 82.353 34 6 0 2 35 179 212 3.94e-11 57.4
Msa0753790 AT2G02148.5 82.353 34 6 0 2 35 179 212 4.72e-11 57.0
Msa0753790 AT2G02148.4 82.353 34 6 0 2 35 179 212 4.72e-11 57.0
Msa0753790 AT2G02148.1 82.353 34 6 0 2 35 179 212 4.72e-11 57.0
Msa0753790 AT2G02148.3 82.353 34 6 0 2 35 179 212 4.72e-11 57.0

Find 17 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCATGATGTTGCCTCATTT+TGG 0.197106 5_2:+7397932 Msa0753790:CDS
ATCATGCTTAAAGGGATATC+TGG 0.221086 5_2:-7397917 None:intergenic
AAAGGGATATCTGGGAAATT+TGG 0.243233 5_2:-7397908 None:intergenic
TGAGGCAACATCATGCTTAA+AGG 0.260855 5_2:-7397926 None:intergenic
ATTATGCTTGCTTCGTTGAA+TGG 0.390277 5_2:+7396667 Msa0753790:exon
GAGGCAACATCATGCTTAAA+GGG 0.411665 5_2:-7397925 None:intergenic
ATGCAGGTGGTTTGCATAGA+GGG 0.485307 5_2:+7396725 Msa0753790:CDS
ACGAGAGAGATTCGGTATGA+AGG 0.490408 5_2:+7396619 Msa0753790:five_prime_UTR
AGAGGAGGACGAGAGAGATT+CGG 0.496065 5_2:+7396611 None:intergenic
CCACGCCTATGCTTGTGTCT+TGG 0.506145 5_2:-7397869 None:intergenic
TCATGCTTAAAGGGATATCT+GGG 0.515190 5_2:-7397916 None:intergenic
GATGCAGGTGGTTTGCATAG+AGG 0.539542 5_2:+7396724 Msa0753790:CDS
CGTGTTGCTCTTGAAGATGC+AGG 0.579378 5_2:+7396709 Msa0753790:CDS
CCAAGACACAAGCATAGGCG+TGG 0.662445 5_2:+7397869 Msa0753790:CDS
ACTCTCCAAGACACAAGCAT+AGG 0.666512 5_2:+7397864 Msa0753790:CDS
GTTGCTCTTGAAGATGCAGG+TGG 0.672716 5_2:+7396712 Msa0753790:CDS
CTGCATCTTCAAGAGCAACA+CGG 0.678512 5_2:-7396708 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTAATGTTTTATTGCTTT+AGG + chr5_2:7396857-7396876 Msa0753790:intron 15.0%
!!! TTTAATGTTTTATTGCTTTA+GGG + chr5_2:7396858-7396877 Msa0753790:intron 15.0%
!!! CATTTAATTTAATGTATTCT+AGG + chr5_2:7396908-7396927 Msa0753790:intron 15.0%
!! GATCAAATTAAAAATAAACA+CGG - chr5_2:7397126-7397145 None:intergenic 15.0%
!! AAAATTCAAACATAATAAAG+AGG - chr5_2:7397423-7397442 None:intergenic 15.0%
!!! TTTATTATGTTTGAATTTTG+TGG + chr5_2:7397424-7397443 Msa0753790:intron 15.0%
!! CATAAAAATTCAAACAAAAA+AGG - chr5_2:7397451-7397470 None:intergenic 15.0%
!!! ATTATTGTGTCAATTTTAAT+TGG + chr5_2:7397592-7397611 Msa0753790:intron 15.0%
!! TTCATTAAAATGTAACAAAA+GGG - chr5_2:7397654-7397673 None:intergenic 15.0%
!!! ATTTAATGTTTTATTGCTTT+AGG + chr5_2:7396857-7396876 Msa0753790:intron 15.0%
!!! TTTAATGTTTTATTGCTTTA+GGG + chr5_2:7396858-7396877 Msa0753790:intron 15.0%
!!! CATTTAATTTAATGTATTCT+AGG + chr5_2:7396908-7396927 Msa0753790:intron 15.0%
!! GATCAAATTAAAAATAAACA+CGG - chr5_2:7397126-7397145 None:intergenic 15.0%
!! AAAATTCAAACATAATAAAG+AGG - chr5_2:7397423-7397442 None:intergenic 15.0%
!!! TTTATTATGTTTGAATTTTG+TGG + chr5_2:7397424-7397443 Msa0753790:intron 15.0%
!! CATAAAAATTCAAACAAAAA+AGG - chr5_2:7397451-7397470 None:intergenic 15.0%
!!! ATTATTGTGTCAATTTTAAT+TGG + chr5_2:7397592-7397611 Msa0753790:intron 15.0%
!! TTCATTAAAATGTAACAAAA+GGG - chr5_2:7397654-7397673 None:intergenic 15.0%
!! ACAAAGAAATTTCCTAAAAA+AGG + chr5_2:7396751-7396770 Msa0753790:CDS 20.0%
!!! ATATAAAAAGGACCTTTTTT+AGG - chr5_2:7396766-7396785 None:intergenic 20.0%
!! AATCTGCATTGAATATAAAA+AGG - chr5_2:7396778-7396797 None:intergenic 20.0%
!!! TGTTTTTAGAGATTGAAAAT+TGG + chr5_2:7397469-7397488 Msa0753790:intron 20.0%
!! CTTCATTAAAATGTAACAAA+AGG - chr5_2:7397655-7397674 None:intergenic 20.0%
!!! CTTTTGTTACATTTTAATGA+AGG + chr5_2:7397653-7397672 Msa0753790:intron 20.0%
!! AATGAGTGTAATAACAAAAT+CGG + chr5_2:7397697-7397716 Msa0753790:intron 20.0%
!! ACAAAGAAATTTCCTAAAAA+AGG + chr5_2:7396751-7396770 Msa0753790:CDS 20.0%
!!! ATATAAAAAGGACCTTTTTT+AGG - chr5_2:7396766-7396785 None:intergenic 20.0%
!! AATCTGCATTGAATATAAAA+AGG - chr5_2:7396778-7396797 None:intergenic 20.0%
!!! TGTTTTTAGAGATTGAAAAT+TGG + chr5_2:7397469-7397488 Msa0753790:intron 20.0%
!! CTTCATTAAAATGTAACAAA+AGG - chr5_2:7397655-7397674 None:intergenic 20.0%
!!! CTTTTGTTACATTTTAATGA+AGG + chr5_2:7397653-7397672 Msa0753790:intron 20.0%
!! AATGAGTGTAATAACAAAAT+CGG + chr5_2:7397697-7397716 Msa0753790:intron 20.0%
! TCTTATTGTATAACTAGTTC+TGG - chr5_2:7397007-7397026 None:intergenic 25.0%
! AAAATTTGCCAATGAAAACA+GGG - chr5_2:7397224-7397243 None:intergenic 25.0%
! AAAAATTTGCCAATGAAAAC+AGG - chr5_2:7397225-7397244 None:intergenic 25.0%
! TTCAAACATAATAAAGAGGA+AGG - chr5_2:7397419-7397438 None:intergenic 25.0%
! AATAACAAAATCGGTTATGA+TGG + chr5_2:7397706-7397725 Msa0753790:intron 25.0%
! ATAACAAAATCGGTTATGAT+GGG + chr5_2:7397707-7397726 Msa0753790:intron 25.0%
!! CATTTCATTTGAAATTTGCA+AGG + chr5_2:7397742-7397761 Msa0753790:intron 25.0%
!!! CTTGCTTTGTTTTTGTTTAT+AGG + chr5_2:7397783-7397802 Msa0753790:intron 25.0%
! TATAGGTTTAAAAGATTGAC+AGG + chr5_2:7397800-7397819 Msa0753790:intron 25.0%
! TCTTATTGTATAACTAGTTC+TGG - chr5_2:7397007-7397026 None:intergenic 25.0%
! AAAATTTGCCAATGAAAACA+GGG - chr5_2:7397224-7397243 None:intergenic 25.0%
! AAAAATTTGCCAATGAAAAC+AGG - chr5_2:7397225-7397244 None:intergenic 25.0%
! TTCAAACATAATAAAGAGGA+AGG - chr5_2:7397419-7397438 None:intergenic 25.0%
! AATAACAAAATCGGTTATGA+TGG + chr5_2:7397706-7397725 Msa0753790:intron 25.0%
! ATAACAAAATCGGTTATGAT+GGG + chr5_2:7397707-7397726 Msa0753790:intron 25.0%
!! CATTTCATTTGAAATTTGCA+AGG + chr5_2:7397742-7397761 Msa0753790:intron 25.0%
!!! CTTGCTTTGTTTTTGTTTAT+AGG + chr5_2:7397783-7397802 Msa0753790:intron 25.0%
! TATAGGTTTAAAAGATTGAC+AGG + chr5_2:7397800-7397819 Msa0753790:intron 25.0%
!! TGCTTTTAGCTATATAGTGT+TGG + chr5_2:7396808-7396827 Msa0753790:intron 30.0%
AGGGTCATATGATGTATTAT+TGG + chr5_2:7396877-7396896 Msa0753790:intron 30.0%
TATTATTTCCTAAGCTCTGT+AGG + chr5_2:7397329-7397348 Msa0753790:intron 30.0%
! ATCTATTTTCTATAGCTCAG+TGG + chr5_2:7397384-7397403 Msa0753790:intron 30.0%
GATTGAAAATTGGTACTATG+CGG + chr5_2:7397479-7397498 Msa0753790:intron 30.0%
AAAATGTAACAAAAGGGAAC+AGG - chr5_2:7397648-7397667 None:intergenic 30.0%
!! TGCTTTTAGCTATATAGTGT+TGG + chr5_2:7396808-7396827 Msa0753790:intron 30.0%
AGGGTCATATGATGTATTAT+TGG + chr5_2:7396877-7396896 Msa0753790:intron 30.0%
TATTATTTCCTAAGCTCTGT+AGG + chr5_2:7397329-7397348 Msa0753790:intron 30.0%
! ATCTATTTTCTATAGCTCAG+TGG + chr5_2:7397384-7397403 Msa0753790:intron 30.0%
GATTGAAAATTGGTACTATG+CGG + chr5_2:7397479-7397498 Msa0753790:intron 30.0%
AAAATGTAACAAAAGGGAAC+AGG - chr5_2:7397648-7397667 None:intergenic 30.0%
ATTATGCTTGCTTCGTTGAA+TGG + chr5_2:7396667-7396686 Msa0753790:exon 35.0%
! TAGCTATATAGTGTTGGAAG+TGG + chr5_2:7396814-7396833 Msa0753790:intron 35.0%
ATATGTACATAGTCATGTCG+AGG - chr5_2:7396939-7396958 None:intergenic 35.0%
GTTCGCTTTGTGGTAATTAA+TGG + chr5_2:7397034-7397053 Msa0753790:intron 35.0%
! AAGCCTTTTCCCATTAAGTT+GGG - chr5_2:7397160-7397179 None:intergenic 35.0%
TTTGAGCTGAAGATCTGTTA+TGG + chr5_2:7397271-7397290 Msa0753790:intron 35.0%
! GTATTCTATCTGTAGTTCTG+TGG + chr5_2:7397531-7397550 Msa0753790:intron 35.0%
AAACAAAGCAAGTGAGACTA+AGG - chr5_2:7397775-7397794 None:intergenic 35.0%
GGTTTAAAAGATTGACAGGT+TGG + chr5_2:7397804-7397823 Msa0753790:intron 35.0%
AAAGGGATATCTGGGAAATT+TGG - chr5_2:7397911-7397930 None:intergenic 35.0%
TCATGCTTAAAGGGATATCT+GGG - chr5_2:7397919-7397938 None:intergenic 35.0%
ATCATGCTTAAAGGGATATC+TGG - chr5_2:7397920-7397939 None:intergenic 35.0%
!! TTGCCTCATTTTGGAAAATG+AGG + chr5_2:7397941-7397960 Msa0753790:CDS 35.0%
ATTATGCTTGCTTCGTTGAA+TGG + chr5_2:7396667-7396686 Msa0753790:exon 35.0%
! TAGCTATATAGTGTTGGAAG+TGG + chr5_2:7396814-7396833 Msa0753790:intron 35.0%
ATATGTACATAGTCATGTCG+AGG - chr5_2:7396939-7396958 None:intergenic 35.0%
GTTCGCTTTGTGGTAATTAA+TGG + chr5_2:7397034-7397053 Msa0753790:intron 35.0%
! AAGCCTTTTCCCATTAAGTT+GGG - chr5_2:7397160-7397179 None:intergenic 35.0%
TTTGAGCTGAAGATCTGTTA+TGG + chr5_2:7397271-7397290 Msa0753790:intron 35.0%
! GTATTCTATCTGTAGTTCTG+TGG + chr5_2:7397531-7397550 Msa0753790:intron 35.0%
AAACAAAGCAAGTGAGACTA+AGG - chr5_2:7397775-7397794 None:intergenic 35.0%
GGTTTAAAAGATTGACAGGT+TGG + chr5_2:7397804-7397823 Msa0753790:intron 35.0%
AAAGGGATATCTGGGAAATT+TGG - chr5_2:7397911-7397930 None:intergenic 35.0%
TCATGCTTAAAGGGATATCT+GGG - chr5_2:7397919-7397938 None:intergenic 35.0%
ATCATGCTTAAAGGGATATC+TGG - chr5_2:7397920-7397939 None:intergenic 35.0%
ACTGAATTCCATGCCTATTG+AGG + chr5_2:7397075-7397094 Msa0753790:intron 40.0%
!!! CTTTTCCCATTAAGTTGGGT+CGG - chr5_2:7397156-7397175 None:intergenic 40.0%
! CAAGCCTTTTCCCATTAAGT+TGG - chr5_2:7397161-7397180 None:intergenic 40.0%
CAACTTAATGGGAAAAGGCT+TGG + chr5_2:7397159-7397178 Msa0753790:intron 40.0%
! TTGTGTTGCCCTGTTTTCAT+TGG + chr5_2:7397213-7397232 Msa0753790:intron 40.0%
GAGGCAACATCATGCTTAAA+GGG - chr5_2:7397928-7397947 None:intergenic 40.0%
TGAGGCAACATCATGCTTAA+AGG - chr5_2:7397929-7397948 None:intergenic 40.0%
! AGCATGATGTTGCCTCATTT+TGG + chr5_2:7397932-7397951 Msa0753790:CDS 40.0%
ACTGAATTCCATGCCTATTG+AGG + chr5_2:7397075-7397094 Msa0753790:intron 40.0%
!!! CTTTTCCCATTAAGTTGGGT+CGG - chr5_2:7397156-7397175 None:intergenic 40.0%
! CAAGCCTTTTCCCATTAAGT+TGG - chr5_2:7397161-7397180 None:intergenic 40.0%
CAACTTAATGGGAAAAGGCT+TGG + chr5_2:7397159-7397178 Msa0753790:intron 40.0%
! TTGTGTTGCCCTGTTTTCAT+TGG + chr5_2:7397213-7397232 Msa0753790:intron 40.0%
GAGGCAACATCATGCTTAAA+GGG - chr5_2:7397928-7397947 None:intergenic 40.0%
TGAGGCAACATCATGCTTAA+AGG - chr5_2:7397929-7397948 None:intergenic 40.0%
! AGCATGATGTTGCCTCATTT+TGG + chr5_2:7397932-7397951 Msa0753790:CDS 40.0%
! ACGAGAGAGATTCGGTATGA+AGG + chr5_2:7396619-7396638 Msa0753790:five_prime_UTR 45.0%
CTGCATCTTCAAGAGCAACA+CGG - chr5_2:7396711-7396730 None:intergenic 45.0%
! ATGCAGGTGGTTTGCATAGA+GGG + chr5_2:7396725-7396744 Msa0753790:CDS 45.0%
TTGCAACTTCAACAGAGACG+AGG + chr5_2:7396972-7396991 Msa0753790:intron 45.0%
!! ATTAAGTTGGGTCGGCTACA+TGG - chr5_2:7397148-7397167 None:intergenic 45.0%
ATGTAGCCGACCCAACTTAA+TGG + chr5_2:7397147-7397166 Msa0753790:intron 45.0%
TGTAGCCGACCCAACTTAAT+GGG + chr5_2:7397148-7397167 Msa0753790:intron 45.0%
CGACCCAACTTAATGGGAAA+AGG + chr5_2:7397154-7397173 Msa0753790:intron 45.0%
TGGCTGCTGCTATACATACT+TGG + chr5_2:7397291-7397310 Msa0753790:intron 45.0%
CCTATAAGCCTACAGAGCTT+AGG - chr5_2:7397340-7397359 None:intergenic 45.0%
CCTAAGCTCTGTAGGCTTAT+AGG + chr5_2:7397337-7397356 Msa0753790:intron 45.0%
ACTCTCCAAGACACAAGCAT+AGG + chr5_2:7397864-7397883 Msa0753790:CDS 45.0%
! ACGAGAGAGATTCGGTATGA+AGG + chr5_2:7396619-7396638 Msa0753790:five_prime_UTR 45.0%
CTGCATCTTCAAGAGCAACA+CGG - chr5_2:7396711-7396730 None:intergenic 45.0%
! ATGCAGGTGGTTTGCATAGA+GGG + chr5_2:7396725-7396744 Msa0753790:CDS 45.0%
TTGCAACTTCAACAGAGACG+AGG + chr5_2:7396972-7396991 Msa0753790:intron 45.0%
!! ATTAAGTTGGGTCGGCTACA+TGG - chr5_2:7397148-7397167 None:intergenic 45.0%
ATGTAGCCGACCCAACTTAA+TGG + chr5_2:7397147-7397166 Msa0753790:intron 45.0%
TGTAGCCGACCCAACTTAAT+GGG + chr5_2:7397148-7397167 Msa0753790:intron 45.0%
CGACCCAACTTAATGGGAAA+AGG + chr5_2:7397154-7397173 Msa0753790:intron 45.0%
TGGCTGCTGCTATACATACT+TGG + chr5_2:7397291-7397310 Msa0753790:intron 45.0%
CCTATAAGCCTACAGAGCTT+AGG - chr5_2:7397340-7397359 None:intergenic 45.0%
CCTAAGCTCTGTAGGCTTAT+AGG + chr5_2:7397337-7397356 Msa0753790:intron 45.0%
ACTCTCCAAGACACAAGCAT+AGG + chr5_2:7397864-7397883 Msa0753790:CDS 45.0%
! CGTGTTGCTCTTGAAGATGC+AGG + chr5_2:7396709-7396728 Msa0753790:CDS 50.0%
GTTGCTCTTGAAGATGCAGG+TGG + chr5_2:7396712-7396731 Msa0753790:CDS 50.0%
! GATGCAGGTGGTTTGCATAG+AGG + chr5_2:7396724-7396743 Msa0753790:CDS 50.0%
CTTCAACAGAGACGAGGACT+AGG + chr5_2:7396978-7396997 Msa0753790:intron 50.0%
! AGAGAAGCGTGTTCGCTTTG+TGG + chr5_2:7397024-7397043 Msa0753790:intron 50.0%
! CGTGTTGCTCTTGAAGATGC+AGG + chr5_2:7396709-7396728 Msa0753790:CDS 50.0%
GTTGCTCTTGAAGATGCAGG+TGG + chr5_2:7396712-7396731 Msa0753790:CDS 50.0%
! GATGCAGGTGGTTTGCATAG+AGG + chr5_2:7396724-7396743 Msa0753790:CDS 50.0%
CTTCAACAGAGACGAGGACT+AGG + chr5_2:7396978-7396997 Msa0753790:intron 50.0%
! AGAGAAGCGTGTTCGCTTTG+TGG + chr5_2:7397024-7397043 Msa0753790:intron 50.0%
CTCAGCGTCCTCAATAGGCA+TGG - chr5_2:7397086-7397105 None:intergenic 55.0%
TGCCTATTGAGGACGCTGAG+TGG + chr5_2:7397086-7397105 Msa0753790:intron 55.0%
CACCACTCAGCGTCCTCAAT+AGG - chr5_2:7397091-7397110 None:intergenic 55.0%
CCACGCCTATGCTTGTGTCT+TGG - chr5_2:7397872-7397891 None:intergenic 55.0%
CCAAGACACAAGCATAGGCG+TGG + chr5_2:7397869-7397888 Msa0753790:CDS 55.0%
CTCAGCGTCCTCAATAGGCA+TGG - chr5_2:7397086-7397105 None:intergenic 55.0%
TGCCTATTGAGGACGCTGAG+TGG + chr5_2:7397086-7397105 Msa0753790:intron 55.0%
CACCACTCAGCGTCCTCAAT+AGG - chr5_2:7397091-7397110 None:intergenic 55.0%
CCACGCCTATGCTTGTGTCT+TGG - chr5_2:7397872-7397891 None:intergenic 55.0%
CCAAGACACAAGCATAGGCG+TGG + chr5_2:7397869-7397888 Msa0753790:CDS 55.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 7396617 7397961 7396617 ID=Msa0753790;Name=Msa0753790
chr5_2 mRNA 7396617 7397961 7396617 ID=Msa0753790-mRNA-1;Parent=Msa0753790;Name=Msa0753790-mRNA-1;_AED=0.48;_eAED=0.51;_QI=53|0|0.5|1|0|0|2|0|80
chr5_2 exon 7396617 7396772 7396617 ID=Msa0753790-mRNA-1:exon:3341;Parent=Msa0753790-mRNA-1
chr5_2 exon 7397822 7397961 7397822 ID=Msa0753790-mRNA-1:exon:3342;Parent=Msa0753790-mRNA-1
chr5_2 five_prime_UTR 7396617 7396669 7396617 ID=Msa0753790-mRNA-1:five_prime_utr;Parent=Msa0753790-mRNA-1
chr5_2 CDS 7396670 7396772 7396670 ID=Msa0753790-mRNA-1:cds;Parent=Msa0753790-mRNA-1
chr5_2 CDS 7397822 7397961 7397822 ID=Msa0753790-mRNA-1:cds;Parent=Msa0753790-mRNA-1
Gene Sequence

>Msa0753790

ATGCTTGCTTCGTTGAATGGTATTCGCAATAAGTCTATCCGTGTTGCTCTTGAAGATGCAGGTGGTTTGCATAGAGGGCTCACAAAGAAATTTCCTAAAAAAGGTTGGAATGAAGTAGCAATCTCAGCTAACGATTACAAATTCTACTCTCCAAGACACAAGCATAGGCGTGGTGCATCAATTTCACTTCCAAATTTCCCAGATATCCCTTTAAGCATGATGTTGCCTCATTTTGGAAAATGA

Protein sequence

>Msa0753790

MLASLNGIRNKSIRVALEDAGGLHRGLTKKFPKKGWNEVAISANDYKFYSPRHKHRRGASISLPNFPDIPLSMMLPHFGK*