AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0601850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0601850 MtrunA17_Chr4g0028971 96.418 698 8 2 1 681 1 698 0.0 1379
Msa0601850 MtrunA17_Chr2g0326761 68.793 721 163 18 1 681 1 699 0.0 947
Msa0601850 MtrunA17_Chr1g0181211 57.536 690 217 14 9 648 24 687 0.0 735
Msa0601850 MtrunA17_Chr1g0199681 57.118 576 197 14 1 538 83 646 0.0 585
Msa0601850 MtrunA17_Chr7g0264171 54.291 536 212 12 9 520 28 554 0.0 546
Msa0601850 MtrunA17_Chr7g0264161 53.623 345 112 6 21 361 1 301 9.93e-119 357
Msa0601850 MtrunA17_Chr3g0115271 48.615 397 188 6 9 402 16 399 5.95e-117 362
Msa0601850 MtrunA17_Chr5g0424931 40.000 480 223 15 12 477 19 447 1.06e-95 305
Msa0601850 MtrunA17_Chr5g0417281 40.342 409 173 10 4 402 3 350 1.35e-85 278
Msa0601850 MtrunA17_Chr5g0436761 39.062 448 189 12 1 429 1 383 3.25e-84 273
Msa0601850 MtrunA17_Chr2g0285461 38.926 447 205 10 2 436 13 403 2.52e-83 281
Msa0601850 MtrunA17_Chr5g0433301 39.186 393 186 7 5 388 17 365 1.42e-79 270
Msa0601850 MtrunA17_Chr1g0156321 40.992 383 170 8 9 381 40 376 3.02e-78 267
Msa0601850 MtrunA17_Chr2g0289221 40.541 370 138 9 9 361 17 321 1.32e-77 252
Msa0601850 MtrunA17_Chr4g0046371 40.104 384 171 9 12 381 38 376 4.12e-77 261
Msa0601850 MtrunA17_Chr8g0376771 37.978 445 211 12 2 436 13 402 5.00e-77 265
Msa0601850 MtrunA17_Chr2g0277461 36.919 409 191 10 3 402 4 354 1.15e-76 253
Msa0601850 MtrunA17_Chr8g0385791 38.847 399 185 7 12 399 59 409 1.60e-76 262
Msa0601850 MtrunA17_Chr3g0109391 37.740 416 204 8 6 412 20 389 3.41e-76 261
Msa0601850 MtrunA17_Chr8g0346711 32.000 700 359 25 3 654 16 646 5.08e-74 252
Msa0601850 MtrunA17_Chr1g0178011 36.946 406 186 10 9 401 9 357 3.58e-73 243
Msa0601850 MtrunA17_Chr5g0417211 37.437 398 173 8 9 395 9 341 4.16e-72 239
Msa0601850 MtrunA17_Chr4g0070181 38.015 413 193 10 3 404 14 374 5.41e-72 253
Msa0601850 MtrunA17_Chr2g0301251 38.010 392 184 7 3 383 14 357 1.60e-71 251
Msa0601850 MtrunA17_Chr2g0276781 37.204 465 202 14 12 440 23 433 3.38e-71 244
Msa0601850 MtrunA17_Chr4g0029671 37.956 411 187 6 12 402 54 416 4.07e-71 246
Msa0601850 MtrunA17_Chr2g0326281 37.123 431 192 10 12 410 56 439 9.93e-70 243
Msa0601850 MtrunA17_Chr7g0239061 37.081 418 196 13 1 404 3 367 1.96e-67 234
Msa0601850 MtrunA17_Chr8g0389081 36.735 392 195 7 2 384 22 369 1.61e-63 228
Msa0601850 MtrunA17_Chr2g0282961 48.826 213 100 2 6 209 47 259 1.05e-57 206
Msa0601850 MtrunA17_Chr2g0282961 37.963 108 66 1 274 381 281 387 1.88e-18 89.7
Msa0601850 MtrunA17_Chr5g0425261 35.930 398 170 14 9 387 9 340 8.78e-57 197
Msa0601850 MtrunA17_Chr5g0424801 35.969 392 169 14 9 382 9 336 3.60e-56 196
Msa0601850 MtrunA17_Chr4g0009751 49.528 212 99 4 7 211 14 224 1.90e-53 194
Msa0601850 MtrunA17_Chr5g0424741 31.628 430 202 14 9 398 9 386 2.65e-48 177
Msa0601850 MtrunA17_Chr2g0277531 32.162 370 170 13 7 361 15 318 5.61e-48 171
Msa0601850 MtrunA17_Chr5g0425661 43.367 196 93 3 12 196 37 225 3.67e-47 166
Msa0601850 MtrunA17_Chr8g0356211 30.945 307 154 7 116 422 2 250 6.12e-39 146
Msa0601850 MtrunA17_Chr5g0425651 43.220 118 60 3 275 388 1 115 1.90e-21 90.5
Msa0601850 MtrunA17_Chr5g0424791 32.432 148 89 4 58 204 14 151 9.30e-16 75.1
Msa0601850 MtrunA17_Chr8g0345771 40.000 100 58 2 103 202 167 264 2.48e-11 66.6
Msa0601850 MtrunA17_Chr1g0178071 32.432 148 83 4 103 238 71 213 3.94e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0601850 AT2G28350.1 56.516 729 230 17 3 681 2 693 0.0 737
Msa0601850 AT4G30080.1 55.425 682 247 13 4 653 12 668 0.0 704
Msa0601850 AT2G28350.2 55.983 702 222 17 3 654 2 666 0.0 698
Msa0601850 AT1G77850.1 45.679 405 189 9 9 404 16 398 1.08e-113 354
Msa0601850 AT1G77850.2 45.679 405 189 9 9 404 16 398 1.24e-113 354
Msa0601850 AT1G59750.4 31.905 677 339 19 12 646 22 618 3.41e-81 271
Msa0601850 AT1G30330.1 40.097 414 193 8 3 407 14 381 4.90e-81 276
Msa0601850 AT1G59750.2 32.000 675 343 19 12 646 22 620 6.36e-81 270
Msa0601850 AT1G59750.1 31.672 682 339 19 12 646 22 623 2.15e-80 269
Msa0601850 AT1G59750.3 31.672 682 339 19 12 646 22 623 2.15e-80 269
Msa0601850 AT1G30330.3 40.049 412 192 8 5 407 18 383 2.27e-80 275
Msa0601850 AT1G30330.2 40.049 412 192 8 5 407 18 383 2.27e-80 275
Msa0601850 AT5G37020.2 39.075 389 184 7 9 388 19 363 7.70e-80 270
Msa0601850 AT5G37020.1 39.075 389 184 7 9 388 19 363 2.04e-79 270
Msa0601850 AT2G33860.1 38.259 379 181 6 12 381 54 388 6.69e-76 255
Msa0601850 AT5G60450.2 38.670 406 184 9 12 400 41 398 1.54e-75 258
Msa0601850 AT5G60450.1 38.670 406 184 9 12 400 65 422 2.15e-75 258
Msa0601850 AT5G62000.2 39.409 406 185 10 9 400 52 410 5.24e-74 256
Msa0601850 AT5G62000.5 39.409 406 185 10 9 400 52 410 5.24e-74 256
Msa0601850 AT5G62000.3 39.409 406 185 10 9 400 52 410 5.24e-74 256
Msa0601850 AT5G62000.1 39.409 406 185 10 9 400 52 410 5.24e-74 256
Msa0601850 AT5G62000.4 39.409 406 185 10 9 400 52 410 5.82e-74 255
Msa0601850 AT2G46530.1 39.267 382 168 12 12 381 21 350 8.28e-68 233
Msa0601850 AT2G46530.3 39.267 382 168 12 12 381 42 371 2.23e-67 233
Msa0601850 AT1G19850.2 37.783 397 174 9 4 381 35 377 2.27e-66 235
Msa0601850 AT1G19850.1 37.783 397 174 9 4 381 46 388 2.27e-66 235
Msa0601850 AT3G61830.1 36.364 429 205 13 4 418 14 388 5.47e-66 228
Msa0601850 AT1G34310.1 29.878 656 356 22 6 644 21 589 2.70e-62 218
Msa0601850 AT1G35520.1 30.243 658 351 23 6 644 21 589 7.51e-62 217
Msa0601850 AT4G23980.1 35.787 394 174 11 8 381 8 342 4.84e-61 216
Msa0601850 AT4G23980.2 35.787 394 174 10 8 381 8 342 5.01e-61 216
Msa0601850 AT1G34390.1 28.829 666 362 21 6 651 21 594 2.13e-60 213
Msa0601850 AT1G35240.1 36.364 396 183 14 6 387 21 361 1.21e-57 206
Msa0601850 AT5G37020.3 39.041 292 133 5 98 388 21 268 1.65e-57 207
Msa0601850 AT1G19220.1 48.869 221 96 4 3 211 14 229 2.33e-57 211
Msa0601850 AT1G19220.1 40.000 130 67 4 286 412 261 382 2.37e-17 87.0
Msa0601850 AT1G35540.1 34.663 401 188 13 6 395 21 358 1.25e-56 203
Msa0601850 AT1G34410.1 35.401 387 184 12 6 381 21 352 2.62e-56 202
Msa0601850 AT5G20730.3 47.059 221 100 4 3 211 15 230 6.76e-56 206
Msa0601850 AT5G20730.3 42.105 114 64 2 286 398 262 374 5.95e-17 85.9
Msa0601850 AT5G20730.2 47.059 221 100 4 3 211 15 230 7.25e-56 206
Msa0601850 AT5G20730.2 42.478 113 64 1 286 398 262 373 3.04e-18 90.1
Msa0601850 AT5G20730.1 47.059 221 100 4 3 211 15 230 7.26e-56 206
Msa0601850 AT5G20730.1 42.105 114 64 2 286 398 262 374 5.39e-17 86.3
Msa0601850 AT2G33860.2 39.051 274 123 4 108 381 2 231 3.42e-54 192
Msa0601850 AT3G61830.2 36.216 370 179 10 52 418 22 337 2.30e-53 193
Msa0601850 AT1G34170.1 33.075 387 195 12 6 381 19 352 3.22e-52 189
Msa0601850 AT2G46530.2 38.768 276 120 6 107 381 36 263 8.18e-47 174
Msa0601850 AT1G34170.2 38.636 220 118 5 6 214 19 232 7.31e-42 159
Msa0601850 AT1G34170.3 38.636 220 118 5 6 214 19 232 2.22e-41 159
Msa0601850 AT1G43950.1 39.336 211 110 6 6 207 21 222 8.94e-38 140
Msa0601850 ATMG00940.1 38.037 163 85 5 195 356 1 148 8.32e-22 93.2
Msa0601850 AT2G36080.4 36.937 111 64 3 103 208 33 142 1.75e-11 63.9
Msa0601850 AT2G36080.2 36.937 111 64 3 103 208 33 142 1.75e-11 63.9
Msa0601850 AT2G36080.3 36.937 111 64 3 103 208 33 142 4.18e-11 63.9
Msa0601850 AT2G36080.1 36.937 111 64 3 103 208 33 142 4.30e-11 64.3

Find 170 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATGATCATGTGAAATTTA+AGG 0.117603 4_2:+44913512 Msa0601850:three_prime_UTR
TTCTAGGATTAGTTGGTTTA+TGG 0.143329 4_2:+44911216 Msa0601850:CDS
ATTCTTCTTAGACAAGAATT+TGG 0.191481 4_2:+44910112 Msa0601850:five_prime_UTR
TCTTTCTTTGTTGGGGTGTT+AGG 0.196783 4_2:+44910172 Msa0601850:five_prime_UTR
TTGAAACTGAAGACTCTTCT+AGG 0.222931 4_2:+44911200 Msa0601850:CDS
ACCCTTGATTTATCACTTCT+TGG 0.257467 4_2:+44912711 Msa0601850:CDS
GGAAAGTGAGAAAGGGTATT+TGG 0.265159 4_2:+44910232 Msa0601850:CDS
TTCTTCAGAGAGTCTGAAAA+TGG 0.265474 4_2:-44909999 None:intergenic
TTGACAATTCTGACAGATTC+AGG 0.268164 4_2:+44913215 Msa0601850:CDS
TTCTCCGTGGCGCCTTCTTC+AGG 0.276881 4_2:+44911285 Msa0601850:CDS
TCATCTTGTGGATGGACTTC+AGG 0.278431 4_2:+44910884 Msa0601850:CDS
CTAATACAAACATTGATCTA+AGG 0.281084 4_2:+44910351 Msa0601850:CDS
AACAATGTAAATTGTGGAAT+TGG 0.287100 4_2:+44910917 Msa0601850:CDS
AGTTGGAATCTTTCTTTGTT+GGG 0.288375 4_2:+44910164 Msa0601850:five_prime_UTR
TAATAATGTTAACCAAGATT+TGG 0.290265 4_2:+44913709 Msa0601850:three_prime_UTR
CAGGCATGATAAGCTTTCTT+TGG 0.290621 4_2:-44912400 None:intergenic
GAGAGTCTGAAAATGGAAAT+TGG 0.293368 4_2:-44909992 None:intergenic
GGGCCAAACAGCCCCTTCTT+TGG 0.299415 4_2:+44912089 Msa0601850:CDS
TGTGGATGGACTTCAGGTTC+TGG 0.309717 4_2:+44910890 Msa0601850:CDS
CACCGGCAACAAGCTTCTTC+TGG 0.313373 4_2:-44910772 None:intergenic
AGAAGGCGCCACGGAGAATT+AGG 0.313389 4_2:-44911280 None:intergenic
GGGAACAATTGCTTCTGTTC+AGG 0.316761 4_2:+44911237 Msa0601850:CDS
ATGAGGATTCAATGGTGTTC+TGG 0.321716 4_2:+44911157 Msa0601850:CDS
TGGAATTCGAAGGGCGAAAA+AGG 0.325157 4_2:+44910838 Msa0601850:CDS
TCTAGGATTAGTTGGTTTAT+GGG 0.340586 4_2:+44911217 Msa0601850:CDS
AATTGTGGAATTGGACCTTT+TGG 0.346313 4_2:+44910926 Msa0601850:CDS
TGGCAACTTGGATTGGATAT+TGG 0.355146 4_2:+44912648 Msa0601850:CDS
AAGTTGGAATCTTTCTTTGT+TGG 0.356899 4_2:+44910163 Msa0601850:five_prime_UTR
TATCATGCCTGTTGAGTATA+GGG 0.358214 4_2:+44912412 Msa0601850:CDS
TAATGAAGCTTCTTCCTTTG+AGG 0.360496 4_2:+44910456 Msa0601850:CDS
AAGAAGAAGAAAACAAATTC+AGG 0.362993 4_2:+44909920 Msa0601850:five_prime_UTR
AGCAACAATAAACTTCAGTT+AGG 0.363663 4_2:+44912294 Msa0601850:CDS
GTGTCTGTCTGTGTTGTTGT+TGG 0.367469 4_2:+44909810 Msa0601850:five_prime_UTR
TTCACCACTGATTCTTTGTA+AGG 0.370999 4_2:+44910382 Msa0601850:CDS
TTCTTCTTAGACAAGAATTT+GGG 0.372036 4_2:+44910113 Msa0601850:five_prime_UTR
GGTTGTTGGTGTTAAATTCA+TGG 0.380615 4_2:+44910403 Msa0601850:CDS
AGTGAGGGCTGAAGATGTTA+TGG 0.385830 4_2:+44911030 Msa0601850:CDS
AAACCAAAGAAGGGGCTGTT+TGG 0.394320 4_2:-44912092 None:intergenic
GAAATTGGAATCATTCACTA+TGG 0.394513 4_2:-44909977 None:intergenic
AGGATGTGCACGGTGAGCTT+TGG 0.396378 4_2:+44910678 Msa0601850:CDS
AAGACTCTTCTAGGATTAGT+TGG 0.399400 4_2:+44911209 Msa0601850:CDS
TTATCATGCCTGTTGAGTAT+AGG 0.406824 4_2:+44912411 Msa0601850:CDS
GAGAATAAGACATTGAGAAA+TGG 0.411485 4_2:+44910974 Msa0601850:CDS
CACTCCCGAGTTCTGTGTTA+AGG 0.419777 4_2:+44911114 Msa0601850:CDS
TTCTTCCTCGGTGGAGTGAA+AGG 0.420088 4_2:-44911990 None:intergenic
AGGTGGTGGTGGTGATGGTT+TGG 0.423416 4_2:+44910502 Msa0601850:CDS
CTGATTCTTTGTAAGGTTGT+TGG 0.426252 4_2:+44910389 Msa0601850:CDS
CTAATGCTGCTGAGTTTAGT+TGG 0.428567 4_2:+44912628 Msa0601850:CDS
GCCTACTACTACAGGTTACA+TGG 0.430758 4_2:+44911880 Msa0601850:intron
GGATAGTAAACAACTTCAAA+TGG 0.432142 4_2:-44911082 None:intergenic
ACGGTGAGCTTTGGAAGTTT+AGG 0.437969 4_2:+44910687 Msa0601850:CDS
AGAAGAAAGAGTGTTAAAGT+TGG 0.438808 4_2:+44910147 Msa0601850:five_prime_UTR
TTCGCCACCACCGTCTGAAC+AGG 0.445782 4_2:-44910656 None:intergenic
TGAGGATTCAATGGTGTTCT+GGG 0.446254 4_2:+44911158 Msa0601850:CDS
AACCAAGAAGTGATAAATCA+AGG 0.446549 4_2:-44912713 None:intergenic
TAAATGTGGGGTCCAAATCT+TGG 0.447589 4_2:-44913721 None:intergenic
TTCAATGGTGTTCTGGGATG+AGG 0.448575 4_2:+44911164 Msa0601850:CDS
ACTGAAGTTTATTGTTGCTA+AGG 0.452992 4_2:-44912290 None:intergenic
GATGAAGGTGGTGGTGGTGA+TGG 0.454860 4_2:+44910497 Msa0601850:CDS
CATTATGCAAATATGCTTAG+AGG 0.456170 4_2:-44913425 None:intergenic
CAGCTATGGCATGCTTGTGC+TGG 0.456859 4_2:+44910260 Msa0601850:CDS
AGCACCTTGATGTGCATAGT+AGG 0.468204 4_2:+44912337 Msa0601850:CDS
TCAAGGTGCTGAATGTTAGT+AGG 0.470920 4_2:-44912324 None:intergenic
AAGTTTAGGCATATTTATAG+AGG 0.471597 4_2:+44910701 Msa0601850:CDS
GCTGAGTTTAGTTGGCAACT+TGG 0.473727 4_2:+44912636 Msa0601850:CDS
AACCAGAAGAAGCTTGTTGC+CGG 0.474099 4_2:+44910770 Msa0601850:CDS
AACTCGGGAGTGCTAGCTCT+TGG 0.477183 4_2:-44911103 None:intergenic
TCAGAGCTTGGTTATGATGA+AGG 0.480927 4_2:+44910482 Msa0601850:CDS
TTGGATGCATCAAACAAAAC+AGG 0.481900 4_2:+44913401 Msa0601850:CDS
TCAATGAAGGAAAGTGAGAA+AGG 0.482138 4_2:+44910224 Msa0601850:exon
CGTCGGCACTTGCTTACAAC+AGG 0.482545 4_2:+44910731 Msa0601850:CDS
ATTGTCAGATAAACCAAAGA+AGG 0.484820 4_2:-44912102 None:intergenic
GTTGGAATCTTTCTTTGTTG+GGG 0.486228 4_2:+44910165 Msa0601850:five_prime_UTR
GGTTATGATGAAGGTGGTGG+TGG 0.486406 4_2:+44910491 Msa0601850:CDS
TGAGTGGAAGAGTGAGAGTG+AGG 0.488015 4_2:+44911014 Msa0601850:CDS
TCACAAAGGTGAACATGGAT+TGG 0.488178 4_2:+44913382 Msa0601850:CDS
GTTTAGTTGGCAACTTGGAT+TGG 0.492268 4_2:+44912641 Msa0601850:CDS
GCAACAATAAACTTCAGTTA+GGG 0.494974 4_2:+44912295 Msa0601850:CDS
AGGGGCTGTTTGGCCCGAGC+TGG 0.496697 4_2:-44912082 None:intergenic
GGTTCTAACAATGTAAATTG+TGG 0.497055 4_2:+44910911 Msa0601850:CDS
CTTTCCGAGGCTTGATTACA+CGG 0.498962 4_2:+44910625 Msa0601850:CDS
AGAAGAGTCTTCAGTTTCAA+AGG 0.502379 4_2:-44911197 None:intergenic
GGAGAATTAGGCCAGCGAAT+AGG 0.504468 4_2:-44911268 None:intergenic
TTGATGGTTCATCTTGTGGA+TGG 0.504684 4_2:+44910876 Msa0601850:CDS
AGAAGATTCTCTTATCAAGA+TGG 0.506507 4_2:+44913617 Msa0601850:three_prime_UTR
AGGTTGTTGATCCTATTCGC+TGG 0.509848 4_2:+44911257 Msa0601850:CDS
AATCTGATGCAAACAATGGT+GGG 0.510020 4_2:+44910567 Msa0601850:CDS
CTATGGCATGCTTGTGCTGG+TGG 0.512929 4_2:+44910263 Msa0601850:CDS
GCATGCTTGTGCTGGTGGAA+TGG 0.513876 4_2:+44910268 Msa0601850:CDS
AGTAAACAACTTCAAATGGT+TGG 0.519885 4_2:-44911078 None:intergenic
TTGTGGAAAGTAGAAAACTT+TGG 0.520773 4_2:-44910312 None:intergenic
TGTTAAGGCTTCAGCTGTGA+AGG 0.522163 4_2:+44911129 Msa0601850:CDS
CCTTTGAGGAATTCAGAGCT+TGG 0.522861 4_2:+44910470 Msa0601850:CDS
AAATCGGTGAAGAACCCTTC+AGG 0.523462 4_2:+44912838 Msa0601850:CDS
GAAGAGTCTTCAGTTTCAAA+GGG 0.524779 4_2:-44911196 None:intergenic
GGAAACCGCAGCTTCTTCCT+CGG 0.526125 4_2:-44912002 None:intergenic
TAATACAAACATTGATCTAA+GGG 0.528805 4_2:+44910352 Msa0601850:CDS
ATATTGTTGTTTACTTAATG+TGG 0.529066 4_2:+44913464 Msa0601850:three_prime_UTR
ATAATGATGCATATATATCA+TGG 0.534101 4_2:+44913673 Msa0601850:three_prime_UTR
ATTTGTTGCTCAGTGAGTAT+TGG 0.534131 4_2:-44912516 None:intergenic
TGAAGCCTTAACACAGAACT+CGG 0.535150 4_2:-44911119 None:intergenic
TCTTCCTCGGTGGAGTGAAA+GGG 0.536181 4_2:-44911989 None:intergenic
GGCTGAGCCACCTGTTCAGA+CGG 0.539988 4_2:+44910646 Msa0601850:CDS
CAATGAAGGAAAGTGAGAAA+GGG 0.542351 4_2:+44910225 Msa0601850:exon
TGTTCAGACGGTGGTGGCGA+AGG 0.543580 4_2:+44910658 Msa0601850:CDS
GCATGTTCAGCATGTCCTTG+TGG 0.548555 4_2:-44910329 None:intergenic
CAGCTGTGAAGGCTGCTATG+AGG 0.549739 4_2:+44911140 Msa0601850:CDS
GCCACCACCGTCTGAACAGG+TGG 0.552294 4_2:-44910653 None:intergenic
GATACTAATTCGACCAACCA+CGG 0.553761 4_2:-44911942 None:intergenic
ATCGTCTCAGCACAGTACCT+TGG 0.557673 4_2:-44910602 None:intergenic
AATTGTGTGTTGGAATTCGA+AGG 0.560435 4_2:+44910828 Msa0601850:CDS
TCTGATGCAAACAATGGTGG+GGG 0.561128 4_2:+44910569 Msa0601850:CDS
ATACTAATTCGACCAACCAC+GGG 0.564828 4_2:-44911941 None:intergenic
GCCACCTGTTCAGACGGTGG+TGG 0.566017 4_2:+44910652 Msa0601850:CDS
ATTGGATATTGGTCATTGCA+AGG 0.566046 4_2:+44912659 Msa0601850:CDS
GGAACTCACAAAGGTGAACA+TGG 0.567844 4_2:+44913377 Msa0601850:CDS
GAAGCGTGTTAGCCCGTGGT+TGG 0.567936 4_2:+44911929 Msa0601850:CDS
TTGTCAGATAAACCAAAGAA+GGG 0.569032 4_2:-44912101 None:intergenic
ATAGTAAAGACAAAGAGAAG+TGG 0.569647 4_2:+44912574 Msa0601850:CDS
CAATCTGATGCAAACAATGG+TGG 0.570087 4_2:+44910566 Msa0601850:CDS
AGTTTAGGCATATTTATAGA+GGG 0.570327 4_2:+44910702 Msa0601850:CDS
ACCAAGAAGTGATAAATCAA+GGG 0.572563 4_2:-44912712 None:intergenic
AAAACTTTGGAGTTCACTTG+AGG 0.572950 4_2:-44910299 None:intergenic
ATCTGATGCAAACAATGGTG+GGG 0.580572 4_2:+44910568 Msa0601850:CDS
AAAGTCTTAGCAAAAGATGC+AGG 0.580849 4_2:-44910539 None:intergenic
CCAAGCTCTGAATTCCTCAA+AGG 0.581047 4_2:-44910470 None:intergenic
ACAGGTGCTGTGAAGCAAAT+CGG 0.584233 4_2:+44912822 Msa0601850:CDS
TTTATAGAGGGACACCGCGT+CGG 0.584417 4_2:+44910714 Msa0601850:CDS
AAATCCTACTATGCACATCA+AGG 0.584801 4_2:-44912341 None:intergenic
TGTTATGGAAGCTGTGAAGT+TGG 0.585494 4_2:+44911045 Msa0601850:CDS
GAGAATGGTGAATTGTGTGT+TGG 0.591957 4_2:+44910818 Msa0601850:CDS
GAGTGGAAGAGTGAGAGTGA+GGG 0.596583 4_2:+44911015 Msa0601850:CDS
TGACGGAAATTGAACATCGA+GGG 0.599791 4_2:-44912042 None:intergenic
TTGCCTGAAAACGTCGGTGA+CGG 0.603464 4_2:-44912059 None:intergenic
TGGAGTCAGAAGATGTTGGA+AGG 0.603517 4_2:+44912688 Msa0601850:CDS
AGGCTGCTATGAGGATTCAA+TGG 0.603915 4_2:+44911149 Msa0601850:CDS
CTCCACCGAGGAAGAAGCTG+CGG 0.604549 4_2:+44911997 Msa0601850:CDS
GGTATTTGGATCCACAGCTA+TGG 0.606132 4_2:+44910246 Msa0601850:CDS
CAACACAGACAGACACACTC+CGG 0.610206 4_2:-44909803 None:intergenic
GGCACTTGCTTACAACAGGA+TGG 0.613049 4_2:+44910735 Msa0601850:CDS
AACCGCAGCTTCTTCCTCGG+TGG 0.613433 4_2:-44911999 None:intergenic
TTCTTGGAGTCAGAAGATGT+TGG 0.615581 4_2:+44912684 Msa0601850:CDS
AAAGGGCTGAAATGAATGAC+TGG 0.619094 4_2:-44911972 None:intergenic
CCTACTACTACAGGTTACAT+GGG 0.623681 4_2:+44911881 Msa0601850:intron
GCTTGTTGCCGGTGATTCTG+TGG 0.623881 4_2:+44910781 Msa0601850:CDS
CTTGGTTATGATGAAGGTGG+TGG 0.625908 4_2:+44910488 Msa0601850:CDS
GACGGAAATTGAACATCGAG+GGG 0.629218 4_2:-44912041 None:intergenic
GCACAAGCATGCCATAGCTG+TGG 0.631502 4_2:-44910257 None:intergenic
TGAGCCACCTGTTCAGACGG+TGG 0.632661 4_2:+44910649 Msa0601850:CDS
GTGCTGAGACGATCTTTCCG+AGG 0.634969 4_2:+44910612 Msa0601850:CDS
TCAGCCGTGTAATCAAGCCT+CGG 0.643917 4_2:-44910629 None:intergenic
ACACAAAAGCAATACCTGAA+GGG 0.644153 4_2:-44912852 None:intergenic
ACGTGAAGCGTGTTAGCCCG+TGG 0.646883 4_2:+44911925 Msa0601850:CDS
AATACCTGAAGAAGGCGCCA+CGG 0.652216 4_2:-44911289 None:intergenic
TCACAGCACCTGTTGCATCA+CGG 0.662294 4_2:-44912812 None:intergenic
GAAGCCTTAACACAGAACTC+GGG 0.673594 4_2:-44911118 None:intergenic
TGGATAACAGGAACTCACAA+AGG 0.673838 4_2:+44913368 Msa0601850:CDS
GTTGTAAGCAAGTGCCGACG+CGG 0.674241 4_2:-44910728 None:intergenic
TGTCAGATAAACCAAAGAAG+GGG 0.674937 4_2:-44912100 None:intergenic
AGCTGGTTGCCTGAAAACGT+CGG 0.679058 4_2:-44912065 None:intergenic
ACAACCTTACAAAGAATCAG+TGG 0.680398 4_2:-44910386 None:intergenic
TTCGCTGGCCTAATTCTCCG+TGG 0.685289 4_2:+44911272 Msa0601850:CDS
GTGACGGAAATTGAACATCG+AGG 0.688190 4_2:-44912043 None:intergenic
CTGATGCAAACAATGGTGGG+GGG 0.689649 4_2:+44910570 Msa0601850:CDS
ATTGTGTGTTGGAATTCGAA+GGG 0.692432 4_2:+44910829 Msa0601850:CDS
GAGCTTGGTTATGATGAAGG+TGG 0.703479 4_2:+44910485 Msa0601850:CDS
ACTCAATCTGATGCAAACAA+TGG 0.706199 4_2:+44910563 Msa0601850:CDS
GTGGTGGCGAAGGATGTGCA+CGG 0.739739 4_2:+44910668 Msa0601850:CDS
ACGGAAATTGAACATCGAGG+GGG 0.745927 4_2:-44912040 None:intergenic
TCAGCCCTTTCACTCCACCG+AGG 0.764350 4_2:+44911985 Msa0601850:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTAAAAATTGAAGTTTTT+TGG - chr4_2:44910069-44910088 None:intergenic 15.0%
!!! ATATGAAGATTTTGAATAAA+GGG - chr4_2:44910094-44910113 None:intergenic 15.0%
!!! AATATGAAGATTTTGAATAA+AGG - chr4_2:44910095-44910114 None:intergenic 15.0%
!!! ATTGTTTTTTAGTTACAATT+TGG + chr4_2:44911309-44911328 Msa0601850:intron 15.0%
!! GAAAAAATATGATAAAAGTT+TGG + chr4_2:44912434-44912453 Msa0601850:CDS 15.0%
!!! ATATGAACAGAATATTTTTA+TGG + chr4_2:44913571-44913590 Msa0601850:three_prime_UTR 15.0%
!! TAATACAAACATTGATCTAA+GGG + chr4_2:44910352-44910371 Msa0601850:CDS 20.0%
!!! TTTGTTATTGTTCAGTTTTA+TGG + chr4_2:44911402-44911421 Msa0601850:intron 20.0%
!! TTAAGAACAATTAGTAGTAT+TGG - chr4_2:44911518-44911537 None:intergenic 20.0%
!! NNNNNNNNNNGTATACATGC+AGG - chr4_2:44912131-44912150 None:intergenic 20.0%
!! GAAATGTCTTATATAATAAG+AGG + chr4_2:44913323-44913342 Msa0601850:intron 20.0%
!!! ATATTGTTGTTTACTTAATG+TGG + chr4_2:44913464-44913483 Msa0601850:three_prime_UTR 20.0%
!! TTATGATCATGTGAAATTTA+AGG + chr4_2:44913512-44913531 Msa0601850:three_prime_UTR 20.0%
!!! ATAAGAGAATCTTCTTTTTT+TGG - chr4_2:44913612-44913631 None:intergenic 20.0%
!! ATAATGATGCATATATATCA+TGG + chr4_2:44913673-44913692 Msa0601850:three_prime_UTR 20.0%
! AAGAAGAAGAAAACAAATTC+AGG + chr4_2:44909920-44909939 Msa0601850:five_prime_UTR 25.0%
! ATTCTTCTTAGACAAGAATT+TGG + chr4_2:44910112-44910131 Msa0601850:five_prime_UTR 25.0%
! TTCTTCTTAGACAAGAATTT+GGG + chr4_2:44910113-44910132 Msa0601850:five_prime_UTR 25.0%
!!! AAGAAAAAGTGTTTCAATGA+AGG + chr4_2:44910211-44910230 Msa0601850:exon 25.0%
! CTAATACAAACATTGATCTA+AGG + chr4_2:44910351-44910370 Msa0601850:CDS 25.0%
! AAGTTTAGGCATATTTATAG+AGG + chr4_2:44910701-44910720 Msa0601850:CDS 25.0%
! AGTTTAGGCATATTTATAGA+GGG + chr4_2:44910702-44910721 Msa0601850:CDS 25.0%
! AACAATGTAAATTGTGGAAT+TGG + chr4_2:44910917-44910936 Msa0601850:CDS 25.0%
! AAAAAAGAAAATGCTCCAAA+AGG - chr4_2:44910944-44910963 None:intergenic 25.0%
! TAAAAAACAATACCTGAAGA+AGG - chr4_2:44911300-44911319 None:intergenic 25.0%
! TTGAACACATCTTTACATAA+GGG + chr4_2:44911342-44911361 Msa0601850:intron 25.0%
! TATTGCTAGTAATGATATTG+TGG + chr4_2:44911583-44911602 Msa0601850:intron 25.0%
! AAAAAAAAAAACCCAGAGTT+GGG - chr4_2:44911707-44911726 None:intergenic 25.0%
! AAAAAAAAAAAACCCAGAGT+TGG - chr4_2:44911708-44911727 None:intergenic 25.0%
!! TTTTTACTCTTGTGATTATG+AGG + chr4_2:44911723-44911742 Msa0601850:intron 25.0%
!!! TTTTGTATGCTTGATTTGAT+TGG + chr4_2:44911777-44911796 Msa0601850:intron 25.0%
!!! AAACATCAGTTTTGTCTTTT+TGG + chr4_2:44912489-44912508 Msa0601850:CDS 25.0%
! TAAGTTTCACAATACATTCT+AGG + chr4_2:44913027-44913046 Msa0601850:intron 25.0%
!! AAAATAGAGTGAAGTCATTT+TGG - chr4_2:44913085-44913104 None:intergenic 25.0%
!! TTTTGCTACTGTACAATAAT+GGG - chr4_2:44913124-44913143 None:intergenic 25.0%
!! ATTTTGCTACTGTACAATAA+TGG - chr4_2:44913125-44913144 None:intergenic 25.0%
!!! TGAACAGAATATTTTTATGG+AGG + chr4_2:44913574-44913593 Msa0601850:three_prime_UTR 25.0%
GAAATTGGAATCATTCACTA+TGG - chr4_2:44909980-44909999 None:intergenic 30.0%
!!! GAAGTTTTTTGGTTTTCTTG+TGG - chr4_2:44910058-44910077 None:intergenic 30.0%
!! AGAAGAAAGAGTGTTAAAGT+TGG + chr4_2:44910147-44910166 Msa0601850:five_prime_UTR 30.0%
AAGTTGGAATCTTTCTTTGT+TGG + chr4_2:44910163-44910182 Msa0601850:five_prime_UTR 30.0%
AGTTGGAATCTTTCTTTGTT+GGG + chr4_2:44910164-44910183 Msa0601850:five_prime_UTR 30.0%
TTGTGGAAAGTAGAAAACTT+TGG - chr4_2:44910315-44910334 None:intergenic 30.0%
! AAAGTTTTCTACTTTCCACA+AGG + chr4_2:44910314-44910333 Msa0601850:CDS 30.0%
CAAAAACTTCATCAGTTTCA+GGG - chr4_2:44910433-44910452 None:intergenic 30.0%
! GGTTCTAACAATGTAAATTG+TGG + chr4_2:44910911-44910930 Msa0601850:CDS 30.0%
GAGAATAAGACATTGAGAAA+TGG + chr4_2:44910974-44910993 Msa0601850:CDS 30.0%
!!! TTGAGAAATGGTTGTTTTGA+TGG + chr4_2:44910986-44911005 Msa0601850:CDS 30.0%
!!! TGTTTTGATGGTAATTTGAG+TGG + chr4_2:44910998-44911017 Msa0601850:CDS 30.0%
AGTAAACAACTTCAAATGGT+TGG - chr4_2:44911081-44911100 None:intergenic 30.0%
GGATAGTAAACAACTTCAAA+TGG - chr4_2:44911085-44911104 None:intergenic 30.0%
!! TTCTAGGATTAGTTGGTTTA+TGG + chr4_2:44911216-44911235 Msa0601850:CDS 30.0%
!! TCTAGGATTAGTTGGTTTAT+GGG + chr4_2:44911217-44911236 Msa0601850:CDS 30.0%
GTTGAACACATCTTTACATA+AGG + chr4_2:44911341-44911360 Msa0601850:intron 30.0%
! AGAACAATTAGTAGTATTGG+AGG - chr4_2:44911515-44911534 None:intergenic 30.0%
TGAAATAACCACTGTTAACT+AGG + chr4_2:44911747-44911766 Msa0601850:intron 30.0%
GAAATAACCACTGTTAACTA+GGG + chr4_2:44911748-44911767 Msa0601850:intron 30.0%
! TTCACGTTTTGTAGTAAATC+AGG - chr4_2:44911912-44911931 None:intergenic 30.0%
TTGTCAGATAAACCAAAGAA+GGG - chr4_2:44912104-44912123 None:intergenic 30.0%
ATTGTCAGATAAACCAAAGA+AGG - chr4_2:44912105-44912124 None:intergenic 30.0%
ACTGAAGTTTATTGTTGCTA+AGG - chr4_2:44912293-44912312 None:intergenic 30.0%
AGCAACAATAAACTTCAGTT+AGG + chr4_2:44912294-44912313 Msa0601850:CDS 30.0%
GCAACAATAAACTTCAGTTA+GGG + chr4_2:44912295-44912314 Msa0601850:CDS 30.0%
!!! ATTTTTTCCCTATACTCAAC+AGG - chr4_2:44912422-44912441 None:intergenic 30.0%
ATAGTAAAGACAAAGAGAAG+TGG + chr4_2:44912574-44912593 Msa0601850:CDS 30.0%
ACCAAGAAGTGATAAATCAA+GGG - chr4_2:44912715-44912734 None:intergenic 30.0%
AACCAAGAAGTGATAAATCA+AGG - chr4_2:44912716-44912735 None:intergenic 30.0%
!!! GTGTCTTTTGCTAGTTTTTT+TGG + chr4_2:44912964-44912983 Msa0601850:intron 30.0%
!!! TTTTGCTAGTTTTTTTGGTC+CGG + chr4_2:44912969-44912988 Msa0601850:intron 30.0%
TTGTAGACTAAACTAACAAC+CGG - chr4_2:44912991-44913010 None:intergenic 30.0%
TAGCAAAATAGTACTACTCT+TGG + chr4_2:44913136-44913155 Msa0601850:intron 30.0%
TAGTACTACTCTTGGATTAA+TGG + chr4_2:44913144-44913163 Msa0601850:intron 30.0%
AGTACTACTCTTGGATTAAT+GGG + chr4_2:44913145-44913164 Msa0601850:intron 30.0%
!! AGGATCTTGATTGTTTTTCA+GGG + chr4_2:44913343-44913362 Msa0601850:intron 30.0%
CATTATGCAAATATGCTTAG+AGG - chr4_2:44913428-44913447 None:intergenic 30.0%
! TTCATATTCAGACTTTTCAG+AGG - chr4_2:44913558-44913577 None:intergenic 30.0%
AGAAGATTCTCTTATCAAGA+TGG + chr4_2:44913617-44913636 Msa0601850:three_prime_UTR 30.0%
! TGAGTGAAGTTTTTGCTTGA+TGG + chr4_2:44909943-44909962 Msa0601850:five_prime_UTR 35.0%
GAGAGTCTGAAAATGGAAAT+TGG - chr4_2:44909995-44910014 None:intergenic 35.0%
TTCTTCAGAGAGTCTGAAAA+TGG - chr4_2:44910002-44910021 None:intergenic 35.0%
GTTGGAATCTTTCTTTGTTG+GGG + chr4_2:44910165-44910184 Msa0601850:five_prime_UTR 35.0%
TCAATGAAGGAAAGTGAGAA+AGG + chr4_2:44910224-44910243 Msa0601850:exon 35.0%
CAATGAAGGAAAGTGAGAAA+GGG + chr4_2:44910225-44910244 Msa0601850:exon 35.0%
!! AAAACTTTGGAGTTCACTTG+AGG - chr4_2:44910302-44910321 None:intergenic 35.0%
!! TTCACCACTGATTCTTTGTA+AGG + chr4_2:44910382-44910401 Msa0601850:CDS 35.0%
ACAACCTTACAAAGAATCAG+TGG - chr4_2:44910389-44910408 None:intergenic 35.0%
CTGATTCTTTGTAAGGTTGT+TGG + chr4_2:44910389-44910408 Msa0601850:CDS 35.0%
!! GGTTGTTGGTGTTAAATTCA+TGG + chr4_2:44910403-44910422 Msa0601850:CDS 35.0%
GCAAAAACTTCATCAGTTTC+AGG - chr4_2:44910434-44910453 None:intergenic 35.0%
TAATGAAGCTTCTTCCTTTG+AGG + chr4_2:44910456-44910475 Msa0601850:CDS 35.0%
AAAGTCTTAGCAAAAGATGC+AGG - chr4_2:44910542-44910561 None:intergenic 35.0%
ACTCAATCTGATGCAAACAA+TGG + chr4_2:44910563-44910582 Msa0601850:CDS 35.0%
AATCTGATGCAAACAATGGT+GGG + chr4_2:44910567-44910586 Msa0601850:CDS 35.0%
!! TGATTCTGTGGTGTTTTTGA+GGG + chr4_2:44910793-44910812 Msa0601850:CDS 35.0%
AATTGTGTGTTGGAATTCGA+AGG + chr4_2:44910828-44910847 Msa0601850:CDS 35.0%
ATTGTGTGTTGGAATTCGAA+GGG + chr4_2:44910829-44910848 Msa0601850:CDS 35.0%
! AATTGTGGAATTGGACCTTT+TGG + chr4_2:44910926-44910945 Msa0601850:CDS 35.0%
GAAGAGTCTTCAGTTTCAAA+GGG - chr4_2:44911199-44911218 None:intergenic 35.0%
AGAAGAGTCTTCAGTTTCAA+AGG - chr4_2:44911200-44911219 None:intergenic 35.0%
TTGAAACTGAAGACTCTTCT+AGG + chr4_2:44911200-44911219 Msa0601850:CDS 35.0%
!! AAGACTCTTCTAGGATTAGT+TGG + chr4_2:44911209-44911228 Msa0601850:CDS 35.0%
!! TTCAGTTTTATGGTCATCTC+TGG + chr4_2:44911412-44911431 Msa0601850:intron 35.0%
TGGATAATCACAAGCAAATG+TGG + chr4_2:44911603-44911622 Msa0601850:intron 35.0%
!! TATCTTTGAGATCGCAATTG+AGG - chr4_2:44911637-44911656 None:intergenic 35.0%
ATTGTTATGCCTACTACTAC+AGG + chr4_2:44911872-44911891 Msa0601850:intron 35.0%
TGTCAGATAAACCAAAGAAG+GGG - chr4_2:44912103-44912122 None:intergenic 35.0%
AAATCCTACTATGCACATCA+AGG - chr4_2:44912344-44912363 None:intergenic 35.0%
TTATCATGCCTGTTGAGTAT+AGG + chr4_2:44912411-44912430 Msa0601850:CDS 35.0%
TATCATGCCTGTTGAGTATA+GGG + chr4_2:44912412-44912431 Msa0601850:CDS 35.0%
! ATTTGTTGCTCAGTGAGTAT+TGG - chr4_2:44912519-44912538 None:intergenic 35.0%
! AGACAAAGAGAAGTGGTTTT+TGG + chr4_2:44912581-44912600 Msa0601850:CDS 35.0%
! TTTTTGGATGCATTTTCAGC+TGG + chr4_2:44912597-44912616 Msa0601850:CDS 35.0%
! TTTTGGATGCATTTTCAGCT+GGG + chr4_2:44912598-44912617 Msa0601850:CDS 35.0%
ATTGGATATTGGTCATTGCA+AGG + chr4_2:44912659-44912678 Msa0601850:CDS 35.0%
ACCCTTGATTTATCACTTCT+TGG + chr4_2:44912711-44912730 Msa0601850:CDS 35.0%
ACACAAAAGCAATACCTGAA+GGG - chr4_2:44912855-44912874 None:intergenic 35.0%
AACACAAAAGCAATACCTGA+AGG - chr4_2:44912856-44912875 None:intergenic 35.0%
AACACCGTCTAACATATTCA+AGG - chr4_2:44912899-44912918 None:intergenic 35.0%
GTACTACTCTTGGATTAATG+GGG + chr4_2:44913146-44913165 Msa0601850:intron 35.0%
TACTACTCTTGGATTAATGG+GGG + chr4_2:44913147-44913166 Msa0601850:intron 35.0%
TTGACAATTCTGACAGATTC+AGG + chr4_2:44913215-44913234 Msa0601850:CDS 35.0%
!! GAGGATCTTGATTGTTTTTC+AGG + chr4_2:44913342-44913361 Msa0601850:intron 35.0%
TTGGATGCATCAAACAAAAC+AGG + chr4_2:44913401-44913420 Msa0601850:CDS 35.0%
! TCTTTCTTTGTTGGGGTGTT+AGG + chr4_2:44910172-44910191 Msa0601850:five_prime_UTR 40.0%
!! GGAAAGTGAGAAAGGGTATT+TGG + chr4_2:44910232-44910251 Msa0601850:CDS 40.0%
TCAGAGCTTGGTTATGATGA+AGG + chr4_2:44910482-44910501 Msa0601850:CDS 40.0%
CAATCTGATGCAAACAATGG+TGG + chr4_2:44910566-44910585 Msa0601850:CDS 40.0%
ATCTGATGCAAACAATGGTG+GGG + chr4_2:44910568-44910587 Msa0601850:CDS 40.0%
CAAAAACACCACAGAATCAC+CGG - chr4_2:44910792-44910811 None:intergenic 40.0%
!! GTGATTCTGTGGTGTTTTTG+AGG + chr4_2:44910792-44910811 Msa0601850:CDS 40.0%
GAGAATGGTGAATTGTGTGT+TGG + chr4_2:44910818-44910837 Msa0601850:CDS 40.0%
!! GGTTTTGATGGTTCATCTTG+TGG + chr4_2:44910872-44910891 Msa0601850:CDS 40.0%
! TTGATGGTTCATCTTGTGGA+TGG + chr4_2:44910876-44910895 Msa0601850:CDS 40.0%
TGTTATGGAAGCTGTGAAGT+TGG + chr4_2:44911045-44911064 Msa0601850:CDS 40.0%
TGAAGCCTTAACACAGAACT+CGG - chr4_2:44911122-44911141 None:intergenic 40.0%
ATGAGGATTCAATGGTGTTC+TGG + chr4_2:44911157-44911176 Msa0601850:CDS 40.0%
TGAGGATTCAATGGTGTTCT+GGG + chr4_2:44911158-44911177 Msa0601850:CDS 40.0%
AGTTCAATTGTCATACTGCG+AGG + chr4_2:44911544-44911563 Msa0601850:intron 40.0%
CAATCTCGGCAACAATATGA+AGG - chr4_2:44911667-44911686 None:intergenic 40.0%
TCATATTGTTGCCGAGATTG+CGG + chr4_2:44911667-44911686 Msa0601850:intron 40.0%
! TGAACTTCTTTTCCCAACTC+TGG + chr4_2:44911692-44911711 Msa0601850:intron 40.0%
! GAACTTCTTTTCCCAACTCT+GGG + chr4_2:44911693-44911712 Msa0601850:intron 40.0%
AACATCTCCCTAGTTAACAG+TGG - chr4_2:44911758-44911777 None:intergenic 40.0%
CCTACTACTACAGGTTACAT+GGG + chr4_2:44911881-44911900 Msa0601850:intron 40.0%
ATACTAATTCGACCAACCAC+GGG - chr4_2:44911944-44911963 None:intergenic 40.0%
GATACTAATTCGACCAACCA+CGG - chr4_2:44911945-44911964 None:intergenic 40.0%
AAAGGGCTGAAATGAATGAC+TGG - chr4_2:44911975-44911994 None:intergenic 40.0%
TGACGGAAATTGAACATCGA+GGG - chr4_2:44912045-44912064 None:intergenic 40.0%
!! TCAAGGTGCTGAATGTTAGT+AGG - chr4_2:44912327-44912346 None:intergenic 40.0%
CAGGCATGATAAGCTTTCTT+TGG - chr4_2:44912403-44912422 None:intergenic 40.0%
CTAATGCTGCTGAGTTTAGT+TGG + chr4_2:44912628-44912647 Msa0601850:CDS 40.0%
GTTTAGTTGGCAACTTGGAT+TGG + chr4_2:44912641-44912660 Msa0601850:CDS 40.0%
! TGGCAACTTGGATTGGATAT+TGG + chr4_2:44912648-44912667 Msa0601850:CDS 40.0%
! TGGTCATTGCAAGGTTTTCT+TGG + chr4_2:44912668-44912687 Msa0601850:CDS 40.0%
TTCTTGGAGTCAGAAGATGT+TGG + chr4_2:44912684-44912703 Msa0601850:CDS 40.0%
!!! GTTTTTTACCGTGATGCAAC+AGG + chr4_2:44912804-44912823 Msa0601850:CDS 40.0%
TCTAACATATTCAAGGACCG+AGG - chr4_2:44912892-44912911 None:intergenic 40.0%
CGGTCCTTGAATATGTTAGA+CGG + chr4_2:44912892-44912911 Msa0601850:intron 40.0%
TGGTCTTTAAACGTGTGTGT+CGG - chr4_2:44913065-44913084 None:intergenic 40.0%
!!! CTTGATTGTTTTTCAGGGCA+TGG + chr4_2:44913348-44913367 Msa0601850:intron 40.0%
!!! TTTTTCAGGGCATGGATAAC+AGG + chr4_2:44913356-44913375 Msa0601850:intron 40.0%
TGGATAACAGGAACTCACAA+AGG + chr4_2:44913368-44913387 Msa0601850:CDS 40.0%
TCACAAAGGTGAACATGGAT+TGG + chr4_2:44913382-44913401 Msa0601850:CDS 40.0%
! GTGTCTGTCTGTGTTGTTGT+TGG + chr4_2:44909810-44909829 Msa0601850:five_prime_UTR 45.0%
GGTATTTGGATCCACAGCTA+TGG + chr4_2:44910246-44910265 Msa0601850:CDS 45.0%
!! CCAAGCTCTGAATTCCTCAA+AGG - chr4_2:44910473-44910492 None:intergenic 45.0%
! CCTTTGAGGAATTCAGAGCT+TGG + chr4_2:44910470-44910489 Msa0601850:CDS 45.0%
GAGCTTGGTTATGATGAAGG+TGG + chr4_2:44910485-44910504 Msa0601850:CDS 45.0%
CTTGGTTATGATGAAGGTGG+TGG + chr4_2:44910488-44910507 Msa0601850:CDS 45.0%
TCTGATGCAAACAATGGTGG+GGG + chr4_2:44910569-44910588 Msa0601850:CDS 45.0%
CTTTCCGAGGCTTGATTACA+CGG + chr4_2:44910625-44910644 Msa0601850:CDS 45.0%
!! ACGGTGAGCTTTGGAAGTTT+AGG + chr4_2:44910687-44910706 Msa0601850:CDS 45.0%
!! AACCAGAAGAAGCTTGTTGC+CGG + chr4_2:44910770-44910789 Msa0601850:CDS 45.0%
!! GTGTTTTTGAGGGCTGAGAA+TGG + chr4_2:44910803-44910822 Msa0601850:CDS 45.0%
TGGAATTCGAAGGGCGAAAA+AGG + chr4_2:44910838-44910857 Msa0601850:CDS 45.0%
GGAATTCGAAGGGCGAAAAA+GGG + chr4_2:44910839-44910858 Msa0601850:CDS 45.0%
!!! GGAATTGGTGGTGGTTTTGA+TGG + chr4_2:44910860-44910879 Msa0601850:CDS 45.0%
! TCATCTTGTGGATGGACTTC+AGG + chr4_2:44910884-44910903 Msa0601850:CDS 45.0%
AGTGAGGGCTGAAGATGTTA+TGG + chr4_2:44911030-44911049 Msa0601850:CDS 45.0%
GAAGCCTTAACACAGAACTC+GGG - chr4_2:44911121-44911140 None:intergenic 45.0%
TGTTAAGGCTTCAGCTGTGA+AGG + chr4_2:44911129-44911148 Msa0601850:CDS 45.0%
AGGCTGCTATGAGGATTCAA+TGG + chr4_2:44911149-44911168 Msa0601850:CDS 45.0%
!! TTCAATGGTGTTCTGGGATG+AGG + chr4_2:44911164-44911183 Msa0601850:CDS 45.0%
GGGAACAATTGCTTCTGTTC+AGG + chr4_2:44911237-44911256 Msa0601850:CDS 45.0%
AGGTTGTTGATCCTATTCGC+TGG + chr4_2:44911257-44911276 Msa0601850:CDS 45.0%
ATGGTCATCTCTGGTGTTTG+TGG + chr4_2:44911421-44911440 Msa0601850:intron 45.0%
AGAAGTTCACACCGCAATCT+CGG - chr4_2:44911681-44911700 None:intergenic 45.0%
GCCTACTACTACAGGTTACA+TGG + chr4_2:44911880-44911899 Msa0601850:intron 45.0%
CCCATGTAACCTGTAGTAGT+AGG - chr4_2:44911884-44911903 None:intergenic 45.0%
ACGGAAATTGAACATCGAGG+GGG - chr4_2:44912043-44912062 None:intergenic 45.0%
GACGGAAATTGAACATCGAG+GGG - chr4_2:44912044-44912063 None:intergenic 45.0%
! GTGACGGAAATTGAACATCG+AGG - chr4_2:44912046-44912065 None:intergenic 45.0%
AAACCAAAGAAGGGGCTGTT+TGG - chr4_2:44912095-44912114 None:intergenic 45.0%
AGCACCTTGATGTGCATAGT+AGG + chr4_2:44912337-44912356 Msa0601850:CDS 45.0%
! GATGCATTTTCAGCTGGGAA+AGG + chr4_2:44912603-44912622 Msa0601850:CDS 45.0%
GCTGAGTTTAGTTGGCAACT+TGG + chr4_2:44912636-44912655 Msa0601850:CDS 45.0%
TGGAGTCAGAAGATGTTGGA+AGG + chr4_2:44912688-44912707 Msa0601850:CDS 45.0%
! ACAGGTGCTGTGAAGCAAAT+CGG + chr4_2:44912822-44912841 Msa0601850:CDS 45.0%
AAATCGGTGAAGAACCCTTC+AGG + chr4_2:44912838-44912857 Msa0601850:CDS 45.0%
TGTTGAGTAAACAAGCGCCT+CGG + chr4_2:44912872-44912891 Msa0601850:intron 45.0%
TCAGGCAGCAAAAATGTGAG+AGG + chr4_2:44913233-44913252 Msa0601850:CDS 45.0%
CAGGCAGCAAAAATGTGAGA+GGG + chr4_2:44913234-44913253 Msa0601850:CDS 45.0%
GGAACTCACAAAGGTGAACA+TGG + chr4_2:44913377-44913396 Msa0601850:CDS 45.0%
GCATGTTCAGCATGTCCTTG+TGG - chr4_2:44910332-44910351 None:intergenic 50.0%
! GGTTATGATGAAGGTGGTGG+TGG + chr4_2:44910491-44910510 Msa0601850:CDS 50.0%
! CTGATGCAAACAATGGTGGG+GGG + chr4_2:44910570-44910589 Msa0601850:CDS 50.0%
ATCGTCTCAGCACAGTACCT+TGG - chr4_2:44910605-44910624 None:intergenic 50.0%
TCAGCCGTGTAATCAAGCCT+CGG - chr4_2:44910632-44910651 None:intergenic 50.0%
TTTATAGAGGGACACCGCGT+CGG + chr4_2:44910714-44910733 Msa0601850:CDS 50.0%
GGCACTTGCTTACAACAGGA+TGG + chr4_2:44910735-44910754 Msa0601850:CDS 50.0%
CGAAGGGCGAAAAAGGGAAT+TGG + chr4_2:44910845-44910864 Msa0601850:CDS 50.0%
AGGGCGAAAAAGGGAATTGG+TGG + chr4_2:44910848-44910867 Msa0601850:CDS 50.0%
GCGAAAAAGGGAATTGGTGG+TGG + chr4_2:44910851-44910870 Msa0601850:CDS 50.0%
! TGTGGATGGACTTCAGGTTC+TGG + chr4_2:44910890-44910909 Msa0601850:CDS 50.0%
TGAGTGGAAGAGTGAGAGTG+AGG + chr4_2:44911014-44911033 Msa0601850:CDS 50.0%
GAGTGGAAGAGTGAGAGTGA+GGG + chr4_2:44911015-44911034 Msa0601850:CDS 50.0%
CACTCCCGAGTTCTGTGTTA+AGG + chr4_2:44911114-44911133 Msa0601850:CDS 50.0%
GGAGAATTAGGCCAGCGAAT+AGG - chr4_2:44911271-44911290 None:intergenic 50.0%
!! AATACCTGAAGAAGGCGCCA+CGG - chr4_2:44911292-44911311 None:intergenic 50.0%
TCTTCCTCGGTGGAGTGAAA+GGG - chr4_2:44911992-44912011 None:intergenic 50.0%
TTCTTCCTCGGTGGAGTGAA+AGG - chr4_2:44911993-44912012 None:intergenic 50.0%
! TTTCCGTCACCGACGTTTTC+AGG + chr4_2:44912056-44912075 Msa0601850:CDS 50.0%
TTGCCTGAAAACGTCGGTGA+CGG - chr4_2:44912062-44912081 None:intergenic 50.0%
AGCTGGTTGCCTGAAAACGT+CGG - chr4_2:44912068-44912087 None:intergenic 50.0%
!! ACGTTTTCAGGCAACCAGCT+CGG + chr4_2:44912068-44912087 Msa0601850:CDS 50.0%
TCCTCCAAATTGAGCATGCC+TGG - chr4_2:44912250-44912269 None:intergenic 50.0%
TCACAGCACCTGTTGCATCA+CGG - chr4_2:44912815-44912834 None:intergenic 50.0%
GCACAAGCATGCCATAGCTG+TGG - chr4_2:44910260-44910279 None:intergenic 55.0%
CAGCTATGGCATGCTTGTGC+TGG + chr4_2:44910260-44910279 Msa0601850:CDS 55.0%
CTATGGCATGCTTGTGCTGG+TGG + chr4_2:44910263-44910282 Msa0601850:CDS 55.0%
! GCATGCTTGTGCTGGTGGAA+TGG + chr4_2:44910268-44910287 Msa0601850:CDS 55.0%
!! GATGAAGGTGGTGGTGGTGA+TGG + chr4_2:44910497-44910516 Msa0601850:CDS 55.0%
!! AGGTGGTGGTGGTGATGGTT+TGG + chr4_2:44910502-44910521 Msa0601850:CDS 55.0%
! GTGGGGGGTTTTCTGTTCCA+AGG + chr4_2:44910585-44910604 Msa0601850:CDS 55.0%
GTGCTGAGACGATCTTTCCG+AGG + chr4_2:44910612-44910631 Msa0601850:CDS 55.0%
AGGATGTGCACGGTGAGCTT+TGG + chr4_2:44910678-44910697 Msa0601850:CDS 55.0%
!! GTTGTAAGCAAGTGCCGACG+CGG - chr4_2:44910731-44910750 None:intergenic 55.0%
CGTCGGCACTTGCTTACAAC+AGG + chr4_2:44910731-44910750 Msa0601850:CDS 55.0%
CACCGGCAACAAGCTTCTTC+TGG - chr4_2:44910775-44910794 None:intergenic 55.0%
! GCTTGTTGCCGGTGATTCTG+TGG + chr4_2:44910781-44910800 Msa0601850:CDS 55.0%
!! AACTCGGGAGTGCTAGCTCT+TGG - chr4_2:44911106-44911125 None:intergenic 55.0%
CAGCTGTGAAGGCTGCTATG+AGG + chr4_2:44911140-44911159 Msa0601850:CDS 55.0%
! TTCGCTGGCCTAATTCTCCG+TGG + chr4_2:44911272-44911291 Msa0601850:CDS 55.0%
!! AGAAGGCGCCACGGAGAATT+AGG - chr4_2:44911283-44911302 None:intergenic 55.0%
GGAAACCGCAGCTTCTTCCT+CGG - chr4_2:44912005-44912024 None:intergenic 55.0%
!! CGTTTTCAGGCAACCAGCTC+GGG + chr4_2:44912069-44912088 Msa0601850:CDS 55.0%
GGAGCCAGGCATGCTCAATT+TGG + chr4_2:44912243-44912262 Msa0601850:intron 55.0%
GCCAGGCATGCTCAATTTGG+AGG + chr4_2:44912246-44912265 Msa0601850:intron 55.0%
GGCTGAGCCACCTGTTCAGA+CGG + chr4_2:44910646-44910665 Msa0601850:CDS 60.0%
TGAGCCACCTGTTCAGACGG+TGG + chr4_2:44910649-44910668 Msa0601850:CDS 60.0%
TTCGCCACCACCGTCTGAAC+AGG - chr4_2:44910659-44910678 None:intergenic 60.0%
! TGTTCAGACGGTGGTGGCGA+AGG + chr4_2:44910658-44910677 Msa0601850:CDS 60.0%
! GTGGTGGCGAAGGATGTGCA+CGG + chr4_2:44910668-44910687 Msa0601850:CDS 60.0%
TTCTCCGTGGCGCCTTCTTC+AGG + chr4_2:44911285-44911304 Msa0601850:CDS 60.0%
ACGTGAAGCGTGTTAGCCCG+TGG + chr4_2:44911925-44911944 Msa0601850:CDS 60.0%
GAAGCGTGTTAGCCCGTGGT+TGG + chr4_2:44911929-44911948 Msa0601850:CDS 60.0%
TCAGCCCTTTCACTCCACCG+AGG + chr4_2:44911985-44912004 Msa0601850:CDS 60.0%
CTCCACCGAGGAAGAAGCTG+CGG + chr4_2:44911997-44912016 Msa0601850:CDS 60.0%
AACCGCAGCTTCTTCCTCGG+TGG - chr4_2:44912002-44912021 None:intergenic 60.0%
AGGGGGAAGTCGAGGTTTGG+TGG - chr4_2:44912026-44912045 None:intergenic 60.0%
TCGAGGGGGAAGTCGAGGTT+TGG - chr4_2:44912029-44912048 None:intergenic 60.0%
GAACATCGAGGGGGAAGTCG+AGG - chr4_2:44912034-44912053 None:intergenic 60.0%
GGGCCAAACAGCCCCTTCTT+TGG + chr4_2:44912089-44912108 Msa0601850:CDS 60.0%
GCCACCTGTTCAGACGGTGG+TGG + chr4_2:44910652-44910671 Msa0601850:CDS 65.0%
GCCACCACCGTCTGAACAGG+TGG - chr4_2:44910656-44910675 None:intergenic 65.0%
AGGGGCTGTTTGGCCCGAGC+TGG - chr4_2:44912085-44912104 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 44909804 44913730 44909804 ID=Msa0601850;Name=Msa0601850
chr4_2 mRNA 44909804 44913730 44909804 ID=Msa0601850-mRNA-1;Parent=Msa0601850;Name=Msa0601850-mRNA-1;_AED=0.03;_eAED=0.03;_QI=423|0.75|0.6|1|1|1|5|285|681
chr4_2 exon 44909804 44911306 44909804 ID=Msa0601850-mRNA-1:exon:9988;Parent=Msa0601850-mRNA-1
chr4_2 exon 44911894 44912134 44911894 ID=Msa0601850-mRNA-1:exon:9989;Parent=Msa0601850-mRNA-1
chr4_2 exon 44912283 44912859 44912283 ID=Msa0601850-mRNA-1:exon:9990;Parent=Msa0601850-mRNA-1
chr4_2 exon 44913190 44913255 44913190 ID=Msa0601850-mRNA-1:exon:9991;Parent=Msa0601850-mRNA-1
chr4_2 exon 44913364 44913730 44913364 ID=Msa0601850-mRNA-1:exon:9992;Parent=Msa0601850-mRNA-1
chr4_2 five_prime_UTR 44909804 44910226 44909804 ID=Msa0601850-mRNA-1:five_prime_utr;Parent=Msa0601850-mRNA-1
chr4_2 CDS 44910227 44911306 44910227 ID=Msa0601850-mRNA-1:cds;Parent=Msa0601850-mRNA-1
chr4_2 CDS 44911894 44912134 44911894 ID=Msa0601850-mRNA-1:cds;Parent=Msa0601850-mRNA-1
chr4_2 CDS 44912283 44912859 44912283 ID=Msa0601850-mRNA-1:cds;Parent=Msa0601850-mRNA-1
chr4_2 CDS 44913190 44913255 44913190 ID=Msa0601850-mRNA-1:cds;Parent=Msa0601850-mRNA-1
chr4_2 CDS 44913364 44913445 44913364 ID=Msa0601850-mRNA-1:cds;Parent=Msa0601850-mRNA-1
chr4_2 three_prime_UTR 44913446 44913730 44913446 ID=Msa0601850-mRNA-1:three_prime_utr;Parent=Msa0601850-mRNA-1
Gene Sequence

>Msa0601850

ATGAAGGAAAGTGAGAAAGGGTATTTGGATCCACAGCTATGGCATGCTTGTGCTGGTGGAATGGTTCAGATACCTCAAGTGAACTCCAAAGTTTTCTACTTTCCACAAGGACATGCTGAACATGCTAATACAAACATTGATCTAAGGGTTTCAGTTTCACCACTGATTCTTTGTAAGGTTGTTGGTGTTAAATTCATGGCTGACCCTGAAACTGATGAAGTTTTTGCTGTAATGAAGCTTCTTCCTTTGAGGAATTCAGAGCTTGGTTATGATGAAGGTGGTGGTGGTGATGGTTTGGAAAATTCAGAAAAGCCTGCATCTTTTGCTAAGACTTTGACTCAATCTGATGCAAACAATGGTGGGGGGTTTTCTGTTCCAAGGTACTGTGCTGAGACGATCTTTCCGAGGCTTGATTACACGGCTGAGCCACCTGTTCAGACGGTGGTGGCGAAGGATGTGCACGGTGAGCTTTGGAAGTTTAGGCATATTTATAGAGGGACACCGCGTCGGCACTTGCTTACAACAGGATGGAGTAGTTTTGTGAACCAGAAGAAGCTTGTTGCCGGTGATTCTGTGGTGTTTTTGAGGGCTGAGAATGGTGAATTGTGTGTTGGAATTCGAAGGGCGAAAAAGGGAATTGGTGGTGGTTTTGATGGTTCATCTTGTGGATGGACTTCAGGTTCTGGTTCTAACAATGTAAATTGTGGAATTGGACCTTTTGGAGCATTTTCTTTTTTCTTGAAAGAAGAGAATAAGACATTGAGAAATGGTTGTTTTGATGGTAATTTGAGTGGAAGAGTGAGAGTGAGGGCTGAAGATGTTATGGAAGCTGTGAAGTTGGCTGCTAATAACCAACCATTTGAAGTTGTTTACTATCCAAGAGCTAGCACTCCCGAGTTCTGTGTTAAGGCTTCAGCTGTGAAGGCTGCTATGAGGATTCAATGGTGTTCTGGGATGAGGTTCAAGATGCCCTTTGAAACTGAAGACTCTTCTAGGATTAGTTGGTTTATGGGAACAATTGCTTCTGTTCAGGTTGTTGATCCTATTCGCTGGCCTAATTCTCCGTGGCGCCTTCTTCAGGTTACATGGGATGAGCCTGATTTACTACAAAACGTGAAGCGTGTTAGCCCGTGGTTGGTCGAATTAGTATCAAATGTACCAGTCATTCATTTCAGCCCTTTCACTCCACCGAGGAAGAAGCTGCGGTTTCCACCAAACCTCGACTTCCCCCTCGATGTTCAATTTCCGTCACCGACGTTTTCAGGCAACCAGCTCGGGCCAAACAGCCCCTTCTTTGGTTTATCTGACAATGCTCCTGCATATTTCAACCTTAGCAACAATAAACTTCAGTTAGGGTTGTTTCCTACTAACATTCAGCACCTTGATGTGCATAGTAGGATTTCAAATAGCAACAACATGATGAACAACAATGATAAGACCAAAGAAAGCTTATCATGCCTGTTGAGTATAGGGAAAAAATATGATAAAAGTTTGGAGAAATCTCAATCTGATGACAATGTTGTTAAGAAACATCAGTTTTGTCTTTTTGGTCAACCAATACTCACTGAGCAACAAATTTCTAGAAACTGTTCAGAAAAAAATTCAGTAGATGATAGTAAAGACAAAGAGAAGTGGTTTTTGGATGCATTTTCAGCTGGGAAAGGTTCTAATGCTGCTGAGTTTAGTTGGCAACTTGGATTGGATATTGGTCATTGCAAGGTTTTCTTGGAGTCAGAAGATGTTGGAAGGACCCTTGATTTATCACTTCTTGGTTCTTATGAAGAGCTCTACAAGAAACTTGCTAACATGTTTGATTTAGAAAAGTCTGAGATGTTGAATCATGTTTTTTACCGTGATGCAACAGGTGCTGTGAAGCAAATCGGTGAAGAACCCTTCAGTGAATTCATGAATACAGCAAAAAGATTGACAATTCTGACAGATTCAGGCAGCAAAAATGTGAGAGGGGCATGGATAACAGGAACTCACAAAGGTGAACATGGATTGGATGCATCAAACAAAACAGGACCTCTAAGCATATTTGCATAA
Protein sequence

>Msa0601850

MKESEKGYLDPQLWHACAGGMVQIPQVNSKVFYFPQGHAEHANTNIDLRVSVSPLILCKVVGVKFMADPETDEVFAVMKLLPLRNSELGYDEGGGGDGLENSEKPASFAKTLTQSDANNGGGFSVPRYCAETIFPRLDYTAEPPVQTVVAKDVHGELWKFRHIYRGTPRRHLLTTGWSSFVNQKKLVAGDSVVFLRAENGELCVGIRRAKKGIGGGFDGSSCGWTSGSGSNNVNCGIGPFGAFSFFLKEENKTLRNGCFDGNLSGRVRVRAEDVMEAVKLAANNQPFEVVYYPRASTPEFCVKASAVKAAMRIQWCSGMRFKMPFETEDSSRISWFMGTIASVQVVDPIRWPNSPWRLLQVTWDEPDLLQNVKRVSPWLVELVSNVPVIHFSPFTPPRKKLRFPPNLDFPLDVQFPSPTFSGNQLGPNSPFFGLSDNAPAYFNLSNNKLQLGLFPTNIQHLDVHSRISNSNNMMNNNDKTKESLSCLLSIGKKYDKSLEKSQSDDNVVKKHQFCLFGQPILTEQQISRNCSEKNSVDDSKDKEKWFLDAFSAGKGSNAAEFSWQLGLDIGHCKVFLESEDVGRTLDLSLLGSYEELYKKLANMFDLEKSEMLNHVFYRDATGAVKQIGEEPFSEFMNTAKRLTILTDSGSKNVRGAWITGTHKGEHGLDASNKTGPLSIFA*