AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0872670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0872670 MtrunA17_Chr6g0453921 93.277 119 6 1 8 126 63 179 9.57e-81 238
Msa0872670 MtrunA17_Chr6g0453801 79.518 83 15 1 8 90 60 140 7.29e-45 146
Msa0872670 MtrunA17_Chr6g0453911 98.438 64 1 0 127 190 1 64 3.51e-39 128
Msa0872670 MtrunA17_Chr6g0453811 83.077 65 10 1 127 190 1 65 1.39e-30 106
Msa0872670 MtrunA17_Chr4g0041861 76.923 65 14 1 127 190 1 65 5.44e-27 97.4
Msa0872670 MtrunA17_Chr5g0440791 48.352 91 46 1 9 98 9 99 7.75e-22 85.9
Msa0872670 MtrunA17_Chr3g0144921 43.396 106 55 3 16 119 62 164 1.46e-19 81.3
Msa0872670 MtrunA17_Chr5g0440804 48.810 84 42 1 16 98 61 144 1.88e-19 80.9
Msa0872670 MtrunA17_Chr5g0440711 48.276 87 42 2 10 95 57 141 2.70e-18 77.4
Msa0872670 MtrunA17_Chr6g0453791 40.909 110 61 2 16 125 62 167 2.24e-17 75.9
Msa0872670 MtrunA17_Chr8g0381481 61.538 65 12 2 127 190 1 53 1.01e-15 68.2
Msa0872670 MtrunA17_Chr4g0001571 39.604 101 52 2 16 109 60 158 3.27e-13 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0872670 AT5G15350.1 57.522 113 46 2 16 126 60 172 6.63e-43 141
Msa0872670 AT5G03850.1 81.250 64 12 0 127 190 1 64 6.58e-31 107
Msa0872670 AT3G10090.1 81.250 64 12 0 127 190 1 64 6.58e-31 107
Msa0872670 AT5G64140.1 81.250 64 12 0 127 190 1 64 1.26e-30 107
Msa0872670 AT4G12880.2 58.904 73 29 1 11 82 16 88 3.02e-23 89.4
Msa0872670 AT4G12880.1 59.701 67 27 0 16 82 60 126 3.49e-22 87.8
Msa0872670 AT3G01070.1 60.577 104 36 4 16 114 58 161 1.71e-21 86.7
Msa0872670 AT2G27035.1 45.783 83 42 2 16 98 60 139 1.84e-16 73.6

Find 41 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGATTCATTCATTTATTTA+TGG 0.202648 6_1:-8243470 Msa0872670:intron
TATATTATGTTTGGAGTTCT+TGG 0.223464 6_1:-8240000 Msa0872670:three_prime_UTR
TATATTATGTTTGGAGTTCT+TGG 0.223464 6_1:-8240134 Msa0872670:three_prime_UTR
TGCAAATGCTTATGATTATT+AGG 0.237384 6_1:-8239960 Msa0872670:three_prime_UTR
TGCAAATGCTTATGATTATT+AGG 0.237384 6_1:-8240094 Msa0872670:three_prime_UTR
GTTTGACTCTATATTATGTT+TGG 0.286542 6_1:-8240009 Msa0872670:three_prime_UTR
GTTTGACTCTATATTATGTT+TGG 0.286542 6_1:-8240143 Msa0872670:three_prime_UTR
AATGACATCTCCTTCTCTAA+TGG 0.346915 6_1:+8240373 None:intergenic
TCAAAACTCAGCCAAATAAA+AGG 0.363604 6_1:+8240044 None:intergenic
TCAAAACTCAGCCAAATAAA+AGG 0.363604 6_1:+8240178 None:intergenic
CCGACCATCCACTTCACAAT+TGG 0.372838 6_1:-8243713 Msa0872670:CDS
TCACTTCTAGTACATTCATT+TGG 0.385696 6_1:+8243763 None:intergenic
CCACTTCACAATTGGACAAC+TGG 0.404662 6_1:-8243705 Msa0872670:CDS
CGCTCAAGTTGAGAAAGTTA+TGG 0.416411 6_1:-8240483 Msa0872670:CDS
ATGACATCTCCTTCTCTAAT+GGG 0.417593 6_1:+8240374 None:intergenic
GCAACATTCCTTTCTTCTAA+AGG 0.419170 6_1:-8243537 Msa0872670:CDS
AGATTTGTCCTTTAGAAGAA+AGG 0.430408 6_1:+8243529 None:intergenic
AAGGTCTAGTAATAATAAAT+AGG 0.443893 6_1:+8240197 None:intergenic
AGTAATAATAAATAGGTCAT+AGG 0.445981 6_1:+8240204 None:intergenic
CACAATTGGACAACTGGAGC+TGG 0.466848 6_1:-8243699 Msa0872670:CDS
TACTATTTCATCAGCGGCAA+AGG 0.470534 6_1:-8243642 Msa0872670:CDS
GCTCAAGTTGAGAAAGTTAT+GGG 0.472061 6_1:-8240482 Msa0872670:CDS
GAGAAAGTTATGGGTCGTAC+TGG 0.491245 6_1:-8240473 Msa0872670:CDS
CTTCTCTCTCAGACTCAAGT+AGG 0.493446 6_1:+8240348 None:intergenic
AAGATATAATAAAGCAATGC+TGG 0.501496 6_1:-8240280 Msa0872670:three_prime_UTR
TAATGGCGAGTCACGTTCAA+CGG 0.551424 6_1:+8243663 None:intergenic
TGAGAAAAGCTACTCTCTAT+CGG 0.552479 6_1:+8240315 None:intergenic
AGAAGAAAGGAATGTTGCAC+CGG 0.561585 6_1:+8243542 None:intergenic
ATCTAGAGGACAGGTCACTC+AGG 0.566307 6_1:-8240450 Msa0872670:CDS
CGCCATTACTATTTCATCAG+CGG 0.567316 6_1:-8243648 Msa0872670:CDS
TCGTACTGGATCTAGAGGAC+AGG 0.594159 6_1:-8240459 Msa0872670:CDS
TGCCGCTGATGAAATAGTAA+TGG 0.603131 6_1:+8243646 None:intergenic
ATGAGAATCGCTACATCATG+AGG 0.604858 6_1:-8240409 Msa0872670:CDS
TTCTCTCTCAGACTCAAGTA+GGG 0.606054 6_1:+8240349 None:intergenic
GTGAAGGGACCCATTAGAGA+AGG 0.608349 6_1:-8240383 Msa0872670:CDS
CCAGTTGTCCAATTGTGAAG+TGG 0.610427 6_1:+8243705 None:intergenic
ATGGGTCGTACTGGATCTAG+AGG 0.632832 6_1:-8240464 Msa0872670:CDS
CTACATCATGAGGAATGTGA+AGG 0.634929 6_1:-8240399 Msa0872670:CDS
TACATCATGAGGAATGTGAA+GGG 0.647475 6_1:-8240398 Msa0872670:CDS
TTGTCCAATTGTGAAGTGGA+TGG 0.661754 6_1:+8243709 None:intergenic
CCAATTGTGAAGTGGATGGT+CGG 0.668169 6_1:+8243713 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTTGTTTAATTTAATTTAT+TGG - chr6_1:8240563-8240582 Msa0872670:intron 10.0%
!! TAAATTATTTATGAAATATG+TGG + chr6_1:8240903-8240922 None:intergenic 10.0%
!!! TTAAAACTTTTGAAATTTAA+AGG + chr6_1:8241321-8241340 None:intergenic 10.0%
!!! AGTCATTTTATTAATTAAAT+TGG - chr6_1:8242164-8242183 Msa0872670:intron 10.0%
!!! GTCATTTTATTAATTAAATT+GGG - chr6_1:8242165-8242184 Msa0872670:intron 10.0%
!!! TAATTTTTTATTTTAAGACT+CGG + chr6_1:8242256-8242275 None:intergenic 10.0%
!! TTTCATAAACAAAAATATTA+AGG + chr6_1:8242879-8242898 None:intergenic 10.0%
!!! TCATTTATAATATTTTGCAT+TGG - chr6_1:8240403-8240422 Msa0872670:CDS 15.0%
!! AAAAAAAATGATCATTTGTA+GGG + chr6_1:8241555-8241574 None:intergenic 15.0%
!! AAAAAAAAATGATCATTTGT+AGG + chr6_1:8241556-8241575 None:intergenic 15.0%
!!! AAATTTTACAATGACAATAT+AGG + chr6_1:8242090-8242109 None:intergenic 15.0%
!!! TATTGTCATTGTAAAATTTT+AGG - chr6_1:8242091-8242110 Msa0872670:intron 15.0%
!!! ATTGTCATTGTAAAATTTTA+GGG - chr6_1:8242092-8242111 Msa0872670:intron 15.0%
!!! AAAATTGTTTTTTTGCAATT+GGG + chr6_1:8242640-8242659 None:intergenic 15.0%
!!! AAAAATTGTTTTTTTGCAAT+TGG + chr6_1:8242641-8242660 None:intergenic 15.0%
!! AAAATAAAAGTAACATACAA+GGG + chr6_1:8242910-8242929 None:intergenic 15.0%
!! AAACTGAAAAAATGAAATCA+AGG + chr6_1:8239971-8239990 None:intergenic 20.0%
!!! TTTATAATATTTTGCATTGG+TGG - chr6_1:8240406-8240425 Msa0872670:CDS 20.0%
!! TTAAGAAACAAGAAAAATGA+TGG + chr6_1:8240689-8240708 None:intergenic 20.0%
!! TTGAATATATAGTTTGATCA+AGG - chr6_1:8240744-8240763 Msa0872670:intron 20.0%
!! ACAAAAATAACTATGATTGA+TGG + chr6_1:8241005-8241024 None:intergenic 20.0%
!! TTGTAAAATGAAACAAGAAT+AGG - chr6_1:8241108-8241127 Msa0872670:intron 20.0%
!!! GCTTTCTAAAATTATGTTTA+TGG - chr6_1:8241794-8241813 Msa0872670:intron 20.0%
!!! TTTTTGCTTATAATAGTAAC+CGG + chr6_1:8241860-8241879 None:intergenic 20.0%
!!! TAAGCAAAAATTGACTTTTT+AGG - chr6_1:8241870-8241889 Msa0872670:intron 20.0%
!!! TAAGCAAAAATTGACTTTTT+AGG + chr6_1:8241870-8241889 None:intergenic 20.0%
!! ACCAACTAAATATGATTATA+AGG - chr6_1:8242134-8242153 Msa0872670:intron 20.0%
!! CCAACTAAATATGATTATAA+GGG - chr6_1:8242135-8242154 Msa0872670:intron 20.0%
!!! TTGCAAAAAAACAATTTTTG+TGG - chr6_1:8242642-8242661 Msa0872670:intron 20.0%
!! CAAAATAAAAGTAACATACA+AGG + chr6_1:8242911-8242930 None:intergenic 20.0%
!! AGTAATAATAAATAGGTCAT+AGG + chr6_1:8243569-8243588 None:intergenic 20.0%
!! AAGGTCTAGTAATAATAAAT+AGG + chr6_1:8243576-8243595 None:intergenic 20.0%
!!! TTATTACTAGACCTTTTATT+TGG - chr6_1:8243581-8243600 Msa0872670:CDS 20.0%
!!! TTATTACTAGACCTTTTATT+TGG - chr6_1:8243581-8243600 Msa0872670:CDS 20.0%
!! TTTCAGTTTTTGTGTATGAT+AGG - chr6_1:8239982-8240001 Msa0872670:three_prime_UTR 25.0%
! GGGATTCATTCATTTATTTA+TGG - chr6_1:8240300-8240319 Msa0872670:three_prime_UTR 25.0%
! TTTATTGGAGAAATCAAATG+CGG - chr6_1:8240578-8240597 Msa0872670:intron 25.0%
!!! GAAATGTTGTTTTATGCTTT+TGG - chr6_1:8240609-8240628 Msa0872670:intron 25.0%
!! TAGTATCAAAAAATGGACTT+AGG + chr6_1:8240713-8240732 None:intergenic 25.0%
! TAGACGATAGTATCAAAAAA+TGG + chr6_1:8240720-8240739 None:intergenic 25.0%
! GAAATTTAAAGGACTAACTT+GGG + chr6_1:8241310-8241329 None:intergenic 25.0%
! TGAAATTTAAAGGACTAACT+TGG + chr6_1:8241311-8241330 None:intergenic 25.0%
!! ATCAAAACACTGCATTTTAA+AGG - chr6_1:8241436-8241455 Msa0872670:intron 25.0%
! AATCAAAACACTGTGTTTAA+TGG + chr6_1:8241580-8241599 None:intergenic 25.0%
! TGTTAAGTTTCAAAGACTTT+GGG + chr6_1:8241604-8241623 None:intergenic 25.0%
! ATGTTAAGTTTCAAAGACTT+TGG + chr6_1:8241605-8241624 None:intergenic 25.0%
!! TAGTTCTTAAGTTGTTGAAT+TGG + chr6_1:8241634-8241653 None:intergenic 25.0%
!!! ATTGACAAGTTTTGATTGAA+TGG + chr6_1:8241715-8241734 None:intergenic 25.0%
!! ACTTGTCAATAAGTTCTTTT+TGG - chr6_1:8241725-8241744 Msa0872670:intron 25.0%
! AAGCAAAAAAAAAACTCAAG+TGG + chr6_1:8241778-8241797 None:intergenic 25.0%
! CATCTAATAAATGATGTATG+TGG + chr6_1:8241933-8241952 None:intergenic 25.0%
!! TTTTTGCTTATAATAGTGAC+CGG - chr6_1:8241968-8241987 Msa0872670:intron 25.0%
! TTTACAATGACAATATAGGT+TGG + chr6_1:8242086-8242105 None:intergenic 25.0%
! CCCTTATAATCATATTTAGT+TGG + chr6_1:8242138-8242157 None:intergenic 25.0%
! CCAAATAACATTAGCATAAA+AGG + chr6_1:8242739-8242758 None:intergenic 25.0%
!! CCTTTTATGCTAATGTTATT+TGG - chr6_1:8242736-8242755 Msa0872670:intron 25.0%
! TGAGATATGATGTATTCAAT+TGG - chr6_1:8242841-8242860 Msa0872670:intron 25.0%
! GAATTGATACTTTAGATTGA+AGG - chr6_1:8242954-8242973 Msa0872670:intron 25.0%
!!! CGGTGTTTAAAAGTTTTAAA+TGG - chr6_1:8243070-8243089 Msa0872670:intron 25.0%
! AAGATATAATAAAGCAATGC+TGG - chr6_1:8243490-8243509 Msa0872670:CDS 25.0%
!!! TGAGTTTTGAGTAAAGATTT+TGG - chr6_1:8243605-8243624 Msa0872670:CDS 25.0%
! GTTTGACTCTATATTATGTT+TGG - chr6_1:8243627-8243646 Msa0872670:CDS 25.0%
!! TATATTATGTTTGGAGTTCT+TGG - chr6_1:8243636-8243655 Msa0872670:CDS 25.0%
! TGCAAATGCTTATGATTATT+AGG - chr6_1:8243676-8243695 Msa0872670:CDS 25.0%
!!! TGAGTTTTGAGTAAAGATTT+TGG - chr6_1:8243605-8243624 Msa0872670:CDS 25.0%
! GTTTGACTCTATATTATGTT+TGG - chr6_1:8243627-8243646 Msa0872670:CDS 25.0%
!! TATATTATGTTTGGAGTTCT+TGG - chr6_1:8243636-8243655 Msa0872670:CDS 25.0%
! TGCAAATGCTTATGATTATT+AGG - chr6_1:8243676-8243695 Msa0872670:CDS 25.0%
TCACTTCTAGTACATTCATT+TGG + chr6_1:8240010-8240029 None:intergenic 30.0%
! AGATTTGTCCTTTAGAAGAA+AGG + chr6_1:8240244-8240263 None:intergenic 30.0%
!!! ATGCCTATTGTTTTTGCTAT+TGG - chr6_1:8240269-8240288 Msa0872670:three_prime_UTR 30.0%
TGAACAAATGTAACACCATT+TGG + chr6_1:8240370-8240389 None:intergenic 30.0%
! TGATTGTTATTGACCTCAAA+TGG - chr6_1:8240429-8240448 Msa0872670:CDS 30.0%
! GATTGTTATTGACCTCAAAT+GGG - chr6_1:8240430-8240449 Msa0872670:CDS 30.0%
! ATTGTTATTGACCTCAAATG+GGG - chr6_1:8240431-8240450 Msa0872670:CDS 30.0%
!!! TGTAGTTTTTAGGTAGTGTT+TGG - chr6_1:8240474-8240493 Msa0872670:CDS 30.0%
GAAATCAAATGCGGAAATTA+TGG - chr6_1:8240587-8240606 Msa0872670:intron 30.0%
ATGTGGATATCTACAAATTG+TGG + chr6_1:8240886-8240905 None:intergenic 30.0%
!! TATTTTTGTCATCCCTAAAC+CGG - chr6_1:8241016-8241035 Msa0872670:intron 30.0%
TTAAAAACAGCACATAGAAC+CGG + chr6_1:8241038-8241057 None:intergenic 30.0%
CAAACTAAAAGGATCAAAAG+TGG + chr6_1:8241279-8241298 None:intergenic 30.0%
CAGTTTAACTGATGTTAATG+TGG - chr6_1:8241393-8241412 Msa0872670:intron 30.0%
GTTACAAATGATCATTTACG+TGG - chr6_1:8241489-8241508 Msa0872670:intron 30.0%
TCAAGTGGTTCATAACTTAA+TGG + chr6_1:8241763-8241782 None:intergenic 30.0%
CATCCAATAAATGATGTATG+TGG - chr6_1:8241895-8241914 Msa0872670:intron 30.0%
AGACCACATACATCATTTAT+TGG + chr6_1:8241927-8241946 None:intergenic 30.0%
AAAATGTGACTAGTAAGATG+AGG - chr6_1:8242193-8242212 Msa0872670:intron 30.0%
AAATGTGACTAGTAAGATGA+GGG - chr6_1:8242194-8242213 Msa0872670:intron 30.0%
TTGTGTGATTTACATGTTTG+TGG - chr6_1:8242703-8242722 Msa0872670:intron 30.0%
AATATTGCGTGTATCATGTT+CGG - chr6_1:8243050-8243069 Msa0872670:intron 30.0%
ATAATCTATGTGACAGAGTA+AGG + chr6_1:8243186-8243205 None:intergenic 30.0%
TCAAAACTCAGCCAAATAAA+AGG + chr6_1:8243595-8243614 None:intergenic 30.0%
TCAAAACTCAGCCAAATAAA+AGG + chr6_1:8243595-8243614 None:intergenic 30.0%
! TGGTAGATTTTCAACACGAA+CGG + chr6_1:8240180-8240199 None:intergenic 35.0%
GCAACATTCCTTTCTTCTAA+AGG - chr6_1:8240233-8240252 Msa0872670:three_prime_UTR 35.0%
GCACCAATAGCAAAAACAAT+AGG + chr6_1:8240275-8240294 None:intergenic 35.0%
CATCCTTAAACAATGCCAAA+TGG - chr6_1:8240352-8240371 Msa0872670:CDS 35.0%
ACACCATTTGGCATTGTTTA+AGG + chr6_1:8240358-8240377 None:intergenic 35.0%
! GTGTGGTGTTTGTAGTTTTT+AGG - chr6_1:8240464-8240483 Msa0872670:CDS 35.0%
!!! TTTTGGACTAGTAGAGTAGT+AGG - chr6_1:8240626-8240645 Msa0872670:intron 35.0%
TAGTTTGAACAGTGTCTAAC+CGG - chr6_1:8240775-8240794 Msa0872670:intron 35.0%
ATATCAACGTTGGTATGGAT+AGG + chr6_1:8240857-8240876 None:intergenic 35.0%
GGGAATTCTGTCAAACTAAA+AGG + chr6_1:8241290-8241309 None:intergenic 35.0%
TTAAACTGAGACTGACAACA+GGG + chr6_1:8241381-8241400 None:intergenic 35.0%
TGTCAAAACGATGCGTTTAA+TGG - chr6_1:8241467-8241486 Msa0872670:intron 35.0%
GTGGTTCATAACTTAATGGT+GGG + chr6_1:8241759-8241778 None:intergenic 35.0%
AGTGGTTCATAACTTAATGG+TGG + chr6_1:8241760-8241779 None:intergenic 35.0%
ACATAAACATATACTCCCTC+CGG - chr6_1:8241838-8241857 Msa0872670:intron 35.0%
TGCTTATAATAGTAACCGGA+GGG + chr6_1:8241856-8241875 None:intergenic 35.0%
TTGCTTATAATAGTAACCGG+AGG + chr6_1:8241857-8241876 None:intergenic 35.0%
AATGTGACTAGTAAGATGAG+GGG - chr6_1:8242195-8242214 Msa0872670:intron 35.0%
! ACTAGTACTTTTCGTGTTCT+TGG - chr6_1:8242222-8242241 Msa0872670:intron 35.0%
AACCTAAGAACAAGGAAGAA+AGG - chr6_1:8242326-8242345 Msa0872670:intron 35.0%
AACCTTTCTTCCTTGTTCTT+AGG + chr6_1:8242331-8242350 None:intergenic 35.0%
!! TTCACGTTGTTGGTTTCAAT+TGG - chr6_1:8242674-8242693 Msa0872670:intron 35.0%
TACATGTTTGTGGTCTCAAT+TGG - chr6_1:8242713-8242732 Msa0872670:intron 35.0%
ACACCTTCAACCTAAAGTAT+CGG + chr6_1:8242792-8242811 None:intergenic 35.0%
AGTGATTGAATGCAATGTGT+CGG + chr6_1:8243015-8243034 None:intergenic 35.0%
! TGGATATTCAGATTGACTTC+AGG - chr6_1:8243090-8243109 Msa0872670:intron 35.0%
ATCACAACATTTGACTCTCA+TGG + chr6_1:8243127-8243146 None:intergenic 35.0%
!! GCTCAAGTTGAGAAAGTTAT+GGG - chr6_1:8243288-8243307 Msa0872670:intron 35.0%
TACATCATGAGGAATGTGAA+GGG - chr6_1:8243372-8243391 Msa0872670:intron 35.0%
ATGACATCTCCTTCTCTAAT+GGG + chr6_1:8243399-8243418 None:intergenic 35.0%
AATGACATCTCCTTCTCTAA+TGG + chr6_1:8243400-8243419 None:intergenic 35.0%
TGAGAAAAGCTACTCTCTAT+CGG + chr6_1:8243458-8243477 None:intergenic 35.0%
! TAGAGAGTAGCTTTTCTCAA+TGG - chr6_1:8243459-8243478 Msa0872670:intron 35.0%
! AGTAGCTTTTCTCAATGGTT+TGG - chr6_1:8243464-8243483 Msa0872670:intron 35.0%
!!! ATAAAGCAATGCTGGTTTTG+TGG - chr6_1:8243498-8243517 Msa0872670:CDS 35.0%
TTGTCCAATTGTGAAGTGGA+TGG + chr6_1:8240064-8240083 None:intergenic 40.0%
CGCCATTACTATTTCATCAG+CGG - chr6_1:8240122-8240141 Msa0872670:three_prime_UTR 40.0%
TGCCGCTGATGAAATAGTAA+TGG + chr6_1:8240127-8240146 None:intergenic 40.0%
TACTATTTCATCAGCGGCAA+AGG - chr6_1:8240128-8240147 Msa0872670:three_prime_UTR 40.0%
AGAAGAAAGGAATGTTGCAC+CGG + chr6_1:8240231-8240250 None:intergenic 40.0%
!!! TTTTTGCTATTGGTGCAGCA+TGG - chr6_1:8240279-8240298 Msa0872670:three_prime_UTR 40.0%
!!! TTTTGCTATTGGTGCAGCAT+GGG - chr6_1:8240280-8240299 Msa0872670:three_prime_UTR 40.0%
TTGACCTCAAATGGGGTTAA+AGG - chr6_1:8240438-8240457 Msa0872670:CDS 40.0%
TGACCTCAAATGGGGTTAAA+GGG - chr6_1:8240439-8240458 Msa0872670:CDS 40.0%
TATCCATTGACATCCCTAGA+AGG + chr6_1:8240806-8240825 None:intergenic 40.0%
GCATGATATCAACGTTGGTA+TGG + chr6_1:8240862-8240881 None:intergenic 40.0%
CTAACAGCATGCGAATAATG+TGG - chr6_1:8241231-8241250 Msa0872670:intron 40.0%
GTTAAACTGAGACTGACAAC+AGG + chr6_1:8241382-8241401 None:intergenic 40.0%
!! ACTGCATTTTAAAGGCTCGA+GGG - chr6_1:8241444-8241463 Msa0872670:intron 40.0%
!! GTCAATGTCATTGACTTGTG+TGG + chr6_1:8241688-8241707 None:intergenic 40.0%
TTGCTTATAATAGTGACCGG+AGG - chr6_1:8241971-8241990 Msa0872670:intron 40.0%
TGCTTATAATAGTGACCGGA+GGG - chr6_1:8241972-8241991 Msa0872670:intron 40.0%
! TTCATTTGAAGTACTCCCTC+CGG + chr6_1:8241990-8242009 None:intergenic 40.0%
TTGTTCTTAGGTTTGTGGTC+TGG + chr6_1:8242319-8242338 None:intergenic 40.0%
AGACCACAAACCTAAGAACA+AGG - chr6_1:8242318-8242337 Msa0872670:intron 40.0%
CTTCCTTGTTCTTAGGTTTG+TGG + chr6_1:8242324-8242343 None:intergenic 40.0%
! ACGAACTTGATGTTGGTGAA+GGG + chr6_1:8242398-8242417 None:intergenic 40.0%
! AACGAACTTGATGTTGGTGA+AGG + chr6_1:8242399-8242418 None:intergenic 40.0%
CAGAGAAACGAACTTGATGT+TGG + chr6_1:8242405-8242424 None:intergenic 40.0%
!! GCTCTTGTTGTGTTTTTGTG+AGG - chr6_1:8242516-8242535 Msa0872670:intron 40.0%
GTTCAAACACTTCACGTTGT+TGG - chr6_1:8242664-8242683 Msa0872670:intron 40.0%
TGTCCAACACTTACTTGCAT+CGG + chr6_1:8242992-8243011 None:intergenic 40.0%
! ATTCAGATTGACTTCAGGCA+CGG - chr6_1:8243095-8243114 Msa0872670:intron 40.0%
GAATGTGTTTGTGTTTGAGC+AGG - chr6_1:8243244-8243263 Msa0872670:intron 40.0%
!! CGCTCAAGTTGAGAAAGTTA+TGG - chr6_1:8243287-8243306 Msa0872670:intron 40.0%
ATGAGAATCGCTACATCATG+AGG - chr6_1:8243361-8243380 Msa0872670:intron 40.0%
CTACATCATGAGGAATGTGA+AGG - chr6_1:8243371-8243390 Msa0872670:intron 40.0%
TTCTCTCTCAGACTCAAGTA+GGG + chr6_1:8243424-8243443 None:intergenic 40.0%
CCAATTGTGAAGTGGATGGT+CGG + chr6_1:8240060-8240079 None:intergenic 45.0%
CCAGTTGTCCAATTGTGAAG+TGG + chr6_1:8240068-8240087 None:intergenic 45.0%
CCACTTCACAATTGGACAAC+TGG - chr6_1:8240065-8240084 Msa0872670:three_prime_UTR 45.0%
TAATGGCGAGTCACGTTCAA+CGG + chr6_1:8240110-8240129 None:intergenic 45.0%
! AGCGGCAAAGGATTTTGCTT+TGG - chr6_1:8240140-8240159 Msa0872670:three_prime_UTR 45.0%
! GGCAAAGGATTTTGCTTTGG+TGG - chr6_1:8240143-8240162 Msa0872670:three_prime_UTR 45.0%
!!! TTTTCAGGTGCAGCTTTTGG+TGG + chr6_1:8240206-8240225 None:intergenic 45.0%
!!! GCTTTTTCAGGTGCAGCTTT+TGG + chr6_1:8240209-8240228 None:intergenic 45.0%
AAAGCTGCACCTGAAAAAGC+CGG - chr6_1:8240209-8240228 Msa0872670:three_prime_UTR 45.0%
!!! AATGTTGCACCGGCTTTTTC+AGG + chr6_1:8240221-8240240 None:intergenic 45.0%
TAGGTCCTTCTTGTCCATGT+GGG - chr6_1:8240645-8240664 Msa0872670:intron 45.0%
! TAGCCTTCTAGGGATGTCAA+TGG - chr6_1:8240800-8240819 Msa0872670:intron 45.0%
! CAGCACATAGAACCGGTTTA+GGG + chr6_1:8241031-8241050 None:intergenic 45.0%
! ACAGCACATAGAACCGGTTT+AGG + chr6_1:8241032-8241051 None:intergenic 45.0%
GAGACTGACAACAGGGATAA+TGG + chr6_1:8241374-8241393 None:intergenic 45.0%
!! CACTGCATTTTAAAGGCTCG+AGG - chr6_1:8241443-8241462 Msa0872670:intron 45.0%
GATCATTTACGTGGCCAGAA+AGG - chr6_1:8241498-8241517 Msa0872670:intron 45.0%
GACAACAAGAACTGCCTTTC+TGG + chr6_1:8241515-8241534 None:intergenic 45.0%
TAACCGCAGCTGCGAAATTA+GGG - chr6_1:8242362-8242381 Msa0872670:intron 45.0%
! AACTTGATGTTGGTGAAGGG+AGG + chr6_1:8242395-8242414 None:intergenic 45.0%
TATAGCACCGCCGATACTTT+AGG - chr6_1:8242779-8242798 Msa0872670:intron 45.0%
!! CCTTCAACCTAAAGTATCGG+CGG + chr6_1:8242789-8242808 None:intergenic 45.0%
TGTCCGATGCAAGTAAGTGT+TGG - chr6_1:8242986-8243005 Msa0872670:intron 45.0%
GTGTTTGTGTTTGAGCAGGA+TGG - chr6_1:8243248-8243267 Msa0872670:intron 45.0%
GAGAAAGTTATGGGTCGTAC+TGG - chr6_1:8243297-8243316 Msa0872670:intron 45.0%
CTTCTCTCTCAGACTCAAGT+AGG + chr6_1:8243425-8243444 None:intergenic 45.0%
CCGACCATCCACTTCACAAT+TGG - chr6_1:8240057-8240076 Msa0872670:three_prime_UTR 50.0%
CACAATTGGACAACTGGAGC+TGG - chr6_1:8240071-8240090 Msa0872670:three_prime_UTR 50.0%
CCACCCTTTAACCCCATTTG+AGG + chr6_1:8240445-8240464 None:intergenic 50.0%
CCTCAAATGGGGTTAAAGGG+TGG - chr6_1:8240442-8240461 Msa0872670:CDS 50.0%
AATGGGGTTAAAGGGTGGTG+TGG - chr6_1:8240447-8240466 Msa0872670:CDS 50.0%
GTAGGTCCTTCTTGTCCATG+TGG - chr6_1:8240644-8240663 Msa0872670:intron 50.0%
TTCCACCCACATGGACAAGA+AGG + chr6_1:8240653-8240672 None:intergenic 50.0%
CAACTAGTGTTCCACCCACA+TGG + chr6_1:8240662-8240681 None:intergenic 50.0%
! TCTAACCGGTCTAGCCTTCT+AGG - chr6_1:8240789-8240808 Msa0872670:intron 50.0%
! CTAACCGGTCTAGCCTTCTA+GGG - chr6_1:8240790-8240809 Msa0872670:intron 50.0%
ACATCCCTAGAAGGCTAGAC+CGG + chr6_1:8240797-8240816 None:intergenic 50.0%
GTGGCGCATGATATCAACGT+TGG + chr6_1:8240867-8240886 None:intergenic 50.0%
GTAACCGCAGCTGCGAAATT+AGG - chr6_1:8242361-8242380 Msa0872670:intron 50.0%
GAACCCTAATTTCGCAGCTG+CGG + chr6_1:8242368-8242387 None:intergenic 50.0%
!! CCGCCGATACTTTAGGTTGA+AGG - chr6_1:8242786-8242805 Msa0872670:intron 50.0%
ATGGGTCGTACTGGATCTAG+AGG - chr6_1:8243306-8243325 Msa0872670:intron 50.0%
TCGTACTGGATCTAGAGGAC+AGG - chr6_1:8243311-8243330 Msa0872670:intron 50.0%
ATCTAGAGGACAGGTCACTC+AGG - chr6_1:8243320-8243339 Msa0872670:intron 50.0%
GTGAAGGGACCCATTAGAGA+AGG - chr6_1:8243387-8243406 Msa0872670:intron 50.0%
! TCAGGTGCAGCTTTTGGTGG+TGG + chr6_1:8240203-8240222 None:intergenic 55.0%
GTCCTTCTTGTCCATGTGGG+TGG - chr6_1:8240648-8240667 Msa0872670:intron 55.0%
GATGTCCGATGCGTGCGTAT+CGG - chr6_1:8242816-8242835 Msa0872670:intron 55.0%
TCAGTCCGATACGCACGCAT+CGG + chr6_1:8242824-8242843 None:intergenic 55.0%
!!! GGTGCAGCTTTTGGTGGTGG+TGG + chr6_1:8240200-8240219 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 8239945 8243847 8239945 ID=Msa0872670;Name=Msa0872670
chr6_1 mRNA 8239945 8243847 8239945 ID=Msa0872670-mRNA-1;Parent=Msa0872670;Name=Msa0872670-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0|0.5|1|1|1|2|386|190
chr6_1 exon 8239945 8240526 8239945 ID=Msa0872670-mRNA-1:exon:2470;Parent=Msa0872670-mRNA-1
chr6_1 exon 8243471 8243847 8243471 ID=Msa0872670-mRNA-1:exon:2469;Parent=Msa0872670-mRNA-1
chr6_1 CDS 8243471 8243847 8243471 ID=Msa0872670-mRNA-1:cds;Parent=Msa0872670-mRNA-1
chr6_1 CDS 8240331 8240526 8240331 ID=Msa0872670-mRNA-1:cds;Parent=Msa0872670-mRNA-1
chr6_1 three_prime_UTR 8239945 8240330 8239945 ID=Msa0872670-mRNA-1:three_prime_utr;Parent=Msa0872670-mRNA-1
Gene Sequence

>Msa0872670

ATGATAATATACTATGTTACTAACCTTGATTTCATTTTTTCAGTTTTTGTGTATGATAGGAACCAAATGAATGTACTAGAAGTGAACAAAACAGATTATGAAACTTGCAATTCCGACCATCCACTTCACAATTGGACAACTGGAGCTGGAAGAGATGTCGTTCCGTTGAACGTGACTCGCCATTACTATTTCATCAGCGGCAAAGGATTTTGCTTTGGTGGTATGAAGTTAGCCGTTCGTGTTGAAAATCTACCACCACCACCAAAAGCTGCACCTGAAAAAGCCGGTGCAACATTCCTTTCTTCTAAAGGACAAATCTTTCTCATGCCTATTGTTTTTGCTATTGGTGCAGCATGGGATTCATTCATTTATTTATGGATGGAATCACAGATTAAGCACGCTCAAGTTGAGAAAGTTATGGGTCGTACTGGATCTAGAGGACAGGTCACTCAGGTTAGAGTCAAGATTCTGCATGAGAATCGCTACATCATGAGGAATGTGAAGGGACCCATTAGAGAAGGAGATGTCATTGCCCTACTTGAGTCTGAGAGAGAAGCAAGAAGACTCCGATAG
Protein sequence

>Msa0872670

MIIYYVTNLDFIFSVFVYDRNQMNVLEVNKTDYETCNSDHPLHNWTTGAGRDVVPLNVTRHYYFISGKGFCFGGMKLAVRVENLPPPPKAAPEKAGATFLSSKGQIFLMPIVFAIGAAWDSFIYLWMESQIKHAQVEKVMGRTGSRGQVTQVRVKILHENRYIMRNVKGPIREGDVIALLESEREARRLR*