AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1126880


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1126880 MtrunA17_Chr7g0270601 61.272 173 21 4 1 127 282 454 1.88e-50 166
Msa1126880 MtrunA17_Chr7g0269191 69.697 99 19 3 40 127 1 99 7.92e-29 101
Msa1126880 MtrunA17_Chr7g0271391 39.167 120 63 3 18 127 504 623 3.99e-22 90.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1126880 AT3G62200.1 36.054 147 74 4 1 127 505 651 3.90e-21 88.2

Find 39 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTCATTTATCGGCAATTT+TGG 0.159196 7_3:+78434540 Msa1126880:CDS
GTACATTCCCATCTGCTTAC+TGG 0.214575 7_3:+78434998 Msa1126880:three_prime_UTR
GTACATTTCAATCTGCTTAC+TGG 0.250536 7_3:+78435058 Msa1126880:three_prime_UTR
CAATTCCTTGCTTCATTTAT+CGG 0.255799 7_3:+78434531 Msa1126880:CDS
CGTGGTTCCCAGACGCAATC+TGG 0.270910 7_3:+78434496 Msa1126880:CDS
TTAGCCTCTGTAAGTGAAAA+TGG 0.275804 7_3:-78434807 None:intergenic
GGGTGATGTACTTAAGATTT+TGG 0.277803 7_3:+78434724 Msa1126880:CDS
GATGACACAGATAGTGATTC+TGG 0.347850 7_3:+78434841 Msa1126880:three_prime_UTR
AAGGTGGTTAAACGAGTCTT+TGG 0.382873 7_3:+78434417 Msa1126880:intron
ATCCTAACCCAGATTGCGTC+TGG 0.396295 7_3:-78434504 None:intergenic
TTATACCTTGTCATTCTAGA+TGG 0.412658 7_3:+78434774 Msa1126880:CDS
GAATACACTGATGAATGAAA+TGG 0.416796 7_3:+78434290 Msa1126880:CDS
CTGTAAGTGAAAATGGTAAT+CGG 0.431067 7_3:-78434800 None:intergenic
TTGTTGCAAATGAGAATCTA+TGG 0.442211 7_3:+78434457 Msa1126880:CDS
TTATAATCACAATCAAGAAA+TGG 0.466378 7_3:+78434750 Msa1126880:CDS
CCTCTACATATCTCTAACTA+CGG 0.467900 7_3:-78434924 None:intergenic
TTGCGTCTGGGAACCACGTA+AGG 0.473407 7_3:-78434491 None:intergenic
TCCCAGACGCAATCTGGGTT+AGG 0.483088 7_3:+78434502 Msa1126880:CDS
AGCAGATGGGAATGTACAAA+AGG 0.496986 7_3:-78434992 None:intergenic
TGTCTTGAAGAGCTTGTGTT+GGG 0.501883 7_3:+78434704 Msa1126880:CDS
CATTTCAATCTGCTTACTGG+TGG 0.505776 7_3:+78435061 Msa1126880:three_prime_UTR
ATTCCCATCTGCTTACTGGT+GGG 0.506945 7_3:+78435002 Msa1126880:three_prime_UTR
TGAGAATTTGCAAGGACTTG+TGG 0.513544 7_3:+78434248 Msa1126880:CDS
CATTCCCATCTGCTTACTGG+TGG 0.519665 7_3:+78435001 Msa1126880:three_prime_UTR
TTCCCATCTGCTTACTGGTG+GGG 0.524628 7_3:+78435003 Msa1126880:three_prime_UTR
GTGTCTTGAAGAGCTTGTGT+TGG 0.527052 7_3:+78434703 Msa1126880:CDS
GTGGTTCCCAGACGCAATCT+GGG 0.539558 7_3:+78434497 Msa1126880:CDS
AGTCCCCACCAGTAAGCAGA+TGG 0.550048 7_3:-78435006 None:intergenic
TCCTAACCCAGATTGCGTCT+GGG 0.566146 7_3:-78434503 None:intergenic
GAGATATGTAGAGGAATTCG+TGG 0.572067 7_3:+78434933 Msa1126880:three_prime_UTR
CCGTAGTTAGAGATATGTAG+AGG 0.580945 7_3:+78434924 Msa1126880:three_prime_UTR
TGCGTCTGGGAACCACGTAA+GGG 0.598064 7_3:-78434490 None:intergenic
GTCCCCACCAGTAAGCAGAT+GGG 0.623279 7_3:-78435005 None:intergenic
AATTGCCGATAAATGAAGCA+AGG 0.623661 7_3:-78434536 None:intergenic
AAACTACGTGAGCTTTGACA+TGG 0.629175 7_3:+78434889 Msa1126880:three_prime_UTR
TCAACACATGAGAATTTGCA+AGG 0.634115 7_3:+78434240 Msa1126880:CDS
GCGTCTGGGAACCACGTAAG+GGG 0.647796 7_3:-78434489 None:intergenic
GGAAGTGTGAACCCCTTACG+TGG 0.663822 7_3:+78434478 Msa1126880:CDS
GGCTGCCATCTAGAATGACA+AGG 0.705174 7_3:-78434779 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACATATAATAAAAGACAAA+AGG - chr7_3:78434645-78434664 None:intergenic 15.0%
!! AACATATAATAAAAGACAAA+AGG - chr7_3:78434645-78434664 None:intergenic 15.0%
!! ATATCAAATGTCTCATATTT+GGG - chr7_3:78434363-78434382 None:intergenic 20.0%
!! AATATCAAATGTCTCATATT+TGG - chr7_3:78434364-78434383 None:intergenic 20.0%
!! AATATGAGACATTTGATATT+AGG + chr7_3:78434364-78434383 Msa1126880:intron 20.0%
!! TTATAATCACAATCAAGAAA+TGG + chr7_3:78434750-78434769 Msa1126880:CDS 20.0%
!! ATATCAAATGTCTCATATTT+GGG - chr7_3:78434363-78434382 None:intergenic 20.0%
!! AATATCAAATGTCTCATATT+TGG - chr7_3:78434364-78434383 None:intergenic 20.0%
!! AATATGAGACATTTGATATT+AGG + chr7_3:78434364-78434383 Msa1126880:intron 20.0%
!! TTATAATCACAATCAAGAAA+TGG + chr7_3:78434750-78434769 Msa1126880:CDS 20.0%
! GCAATCAATTATATTTCCTT+CGG - chr7_3:78434325-78434344 None:intergenic 25.0%
! ATAATTGATTGCATTCGTTA+TGG + chr7_3:78434333-78434352 Msa1126880:intron 25.0%
!!! TATTGTTTTTCTTAGTTCCT+TGG + chr7_3:78434585-78434604 Msa1126880:intron 25.0%
! GCAATCAATTATATTTCCTT+CGG - chr7_3:78434325-78434344 None:intergenic 25.0%
! ATAATTGATTGCATTCGTTA+TGG + chr7_3:78434333-78434352 Msa1126880:intron 25.0%
!!! TATTGTTTTTCTTAGTTCCT+TGG + chr7_3:78434585-78434604 Msa1126880:intron 25.0%
GAATACACTGATGAATGAAA+TGG + chr7_3:78434290-78434309 Msa1126880:CDS 30.0%
TCAATTATATTTCCTTCGGT+AGG - chr7_3:78434321-78434340 None:intergenic 30.0%
TGAGACATTTGATATTAGGA+AGG + chr7_3:78434368-78434387 Msa1126880:intron 30.0%
GAGACATTTGATATTAGGAA+GGG + chr7_3:78434369-78434388 Msa1126880:intron 30.0%
TTGTTGCAAATGAGAATCTA+TGG + chr7_3:78434457-78434476 Msa1126880:CDS 30.0%
CAATTCCTTGCTTCATTTAT+CGG + chr7_3:78434531-78434550 Msa1126880:CDS 30.0%
TAACAAGTAATAAAGCTCCA+AGG - chr7_3:78434605-78434624 None:intergenic 30.0%
TTATACCTTGTCATTCTAGA+TGG + chr7_3:78434774-78434793 Msa1126880:CDS 30.0%
CTGTAAGTGAAAATGGTAAT+CGG - chr7_3:78434803-78434822 None:intergenic 30.0%
! ATTACCATTTTCACTTACAG+AGG + chr7_3:78434803-78434822 Msa1126880:CDS 30.0%
GAATACACTGATGAATGAAA+TGG + chr7_3:78434290-78434309 Msa1126880:CDS 30.0%
TCAATTATATTTCCTTCGGT+AGG - chr7_3:78434321-78434340 None:intergenic 30.0%
TGAGACATTTGATATTAGGA+AGG + chr7_3:78434368-78434387 Msa1126880:intron 30.0%
GAGACATTTGATATTAGGAA+GGG + chr7_3:78434369-78434388 Msa1126880:intron 30.0%
TTGTTGCAAATGAGAATCTA+TGG + chr7_3:78434457-78434476 Msa1126880:CDS 30.0%
CAATTCCTTGCTTCATTTAT+CGG + chr7_3:78434531-78434550 Msa1126880:CDS 30.0%
TAACAAGTAATAAAGCTCCA+AGG - chr7_3:78434605-78434624 None:intergenic 30.0%
TTATACCTTGTCATTCTAGA+TGG + chr7_3:78434774-78434793 Msa1126880:CDS 30.0%
CTGTAAGTGAAAATGGTAAT+CGG - chr7_3:78434803-78434822 None:intergenic 30.0%
! ATTACCATTTTCACTTACAG+AGG + chr7_3:78434803-78434822 Msa1126880:CDS 30.0%
TCAACACATGAGAATTTGCA+AGG + chr7_3:78434240-78434259 Msa1126880:CDS 35.0%
AATTGCCGATAAATGAAGCA+AGG - chr7_3:78434539-78434558 None:intergenic 35.0%
! GCTTCATTTATCGGCAATTT+TGG + chr7_3:78434540-78434559 Msa1126880:CDS 35.0%
! GGGTGATGTACTTAAGATTT+TGG + chr7_3:78434724-78434743 Msa1126880:CDS 35.0%
TTAGCCTCTGTAAGTGAAAA+TGG - chr7_3:78434810-78434829 None:intergenic 35.0%
CCTCTACATATCTCTAACTA+CGG - chr7_3:78434927-78434946 None:intergenic 35.0%
GTACATTTCAATCTGCTTAC+TGG + chr7_3:78435058-78435077 Msa1126880:three_prime_UTR 35.0%
!!! CTTACTGGTGGATTTTTTGA+TGG + chr7_3:78435073-78435092 Msa1126880:three_prime_UTR 35.0%
TCAACACATGAGAATTTGCA+AGG + chr7_3:78434240-78434259 Msa1126880:CDS 35.0%
AATTGCCGATAAATGAAGCA+AGG - chr7_3:78434539-78434558 None:intergenic 35.0%
! GCTTCATTTATCGGCAATTT+TGG + chr7_3:78434540-78434559 Msa1126880:CDS 35.0%
! GGGTGATGTACTTAAGATTT+TGG + chr7_3:78434724-78434743 Msa1126880:CDS 35.0%
TTAGCCTCTGTAAGTGAAAA+TGG - chr7_3:78434810-78434829 None:intergenic 35.0%
CCTCTACATATCTCTAACTA+CGG - chr7_3:78434927-78434946 None:intergenic 35.0%
GTACATTTCAATCTGCTTAC+TGG + chr7_3:78435058-78435077 Msa1126880:three_prime_UTR 35.0%
!!! CTTACTGGTGGATTTTTTGA+TGG + chr7_3:78435073-78435092 Msa1126880:three_prime_UTR 35.0%
TGAGAATTTGCAAGGACTTG+TGG + chr7_3:78434248-78434267 Msa1126880:CDS 40.0%
! GAAATGGTTTTTCCTACCGA+AGG + chr7_3:78434306-78434325 Msa1126880:intron 40.0%
TTGTGCAATTGAGCAACAGA+AGG + chr7_3:78434398-78434417 Msa1126880:intron 40.0%
AAGGTGGTTAAACGAGTCTT+TGG + chr7_3:78434417-78434436 Msa1126880:intron 40.0%
!! CAATTTTGGCGTCTTGTAAC+AGG + chr7_3:78434554-78434573 Msa1126880:intron 40.0%
TGTCTTGAAGAGCTTGTGTT+GGG + chr7_3:78434704-78434723 Msa1126880:CDS 40.0%
GATGACACAGATAGTGATTC+TGG + chr7_3:78434841-78434860 Msa1126880:three_prime_UTR 40.0%
! AAACTACGTGAGCTTTGACA+TGG + chr7_3:78434889-78434908 Msa1126880:three_prime_UTR 40.0%
CCGTAGTTAGAGATATGTAG+AGG + chr7_3:78434924-78434943 Msa1126880:three_prime_UTR 40.0%
GAGATATGTAGAGGAATTCG+TGG + chr7_3:78434933-78434952 Msa1126880:three_prime_UTR 40.0%
AGCAGATGGGAATGTACAAA+AGG - chr7_3:78434995-78435014 None:intergenic 40.0%
CATTTCAATCTGCTTACTGG+TGG + chr7_3:78435061-78435080 Msa1126880:three_prime_UTR 40.0%
TGAGAATTTGCAAGGACTTG+TGG + chr7_3:78434248-78434267 Msa1126880:CDS 40.0%
! GAAATGGTTTTTCCTACCGA+AGG + chr7_3:78434306-78434325 Msa1126880:intron 40.0%
TTGTGCAATTGAGCAACAGA+AGG + chr7_3:78434398-78434417 Msa1126880:intron 40.0%
AAGGTGGTTAAACGAGTCTT+TGG + chr7_3:78434417-78434436 Msa1126880:intron 40.0%
!! CAATTTTGGCGTCTTGTAAC+AGG + chr7_3:78434554-78434573 Msa1126880:intron 40.0%
TGTCTTGAAGAGCTTGTGTT+GGG + chr7_3:78434704-78434723 Msa1126880:CDS 40.0%
GATGACACAGATAGTGATTC+TGG + chr7_3:78434841-78434860 Msa1126880:three_prime_UTR 40.0%
! AAACTACGTGAGCTTTGACA+TGG + chr7_3:78434889-78434908 Msa1126880:three_prime_UTR 40.0%
CCGTAGTTAGAGATATGTAG+AGG + chr7_3:78434924-78434943 Msa1126880:three_prime_UTR 40.0%
GAGATATGTAGAGGAATTCG+TGG + chr7_3:78434933-78434952 Msa1126880:three_prime_UTR 40.0%
AGCAGATGGGAATGTACAAA+AGG - chr7_3:78434995-78435014 None:intergenic 40.0%
CATTTCAATCTGCTTACTGG+TGG + chr7_3:78435061-78435080 Msa1126880:three_prime_UTR 40.0%
TGCAATTGAGCAACAGAAGG+TGG + chr7_3:78434401-78434420 Msa1126880:intron 45.0%
GTGTCTTGAAGAGCTTGTGT+TGG + chr7_3:78434703-78434722 Msa1126880:CDS 45.0%
GTACATTCCCATCTGCTTAC+TGG + chr7_3:78434998-78435017 Msa1126880:three_prime_UTR 45.0%
ATTCCCATCTGCTTACTGGT+GGG + chr7_3:78435002-78435021 Msa1126880:three_prime_UTR 45.0%
! TACTGGTGGGGACTTTTTGT+TGG + chr7_3:78435015-78435034 Msa1126880:three_prime_UTR 45.0%
TGCAATTGAGCAACAGAAGG+TGG + chr7_3:78434401-78434420 Msa1126880:intron 45.0%
GTGTCTTGAAGAGCTTGTGT+TGG + chr7_3:78434703-78434722 Msa1126880:CDS 45.0%
GTACATTCCCATCTGCTTAC+TGG + chr7_3:78434998-78435017 Msa1126880:three_prime_UTR 45.0%
ATTCCCATCTGCTTACTGGT+GGG + chr7_3:78435002-78435021 Msa1126880:three_prime_UTR 45.0%
! TACTGGTGGGGACTTTTTGT+TGG + chr7_3:78435015-78435034 Msa1126880:three_prime_UTR 45.0%
TCCTAACCCAGATTGCGTCT+GGG - chr7_3:78434506-78434525 None:intergenic 50.0%
ATCCTAACCCAGATTGCGTC+TGG - chr7_3:78434507-78434526 None:intergenic 50.0%
! GGCTGCCATCTAGAATGACA+AGG - chr7_3:78434782-78434801 None:intergenic 50.0%
CATTCCCATCTGCTTACTGG+TGG + chr7_3:78435001-78435020 Msa1126880:three_prime_UTR 50.0%
TTCCCATCTGCTTACTGGTG+GGG + chr7_3:78435003-78435022 Msa1126880:three_prime_UTR 50.0%
TCCTAACCCAGATTGCGTCT+GGG - chr7_3:78434506-78434525 None:intergenic 50.0%
ATCCTAACCCAGATTGCGTC+TGG - chr7_3:78434507-78434526 None:intergenic 50.0%
! GGCTGCCATCTAGAATGACA+AGG - chr7_3:78434782-78434801 None:intergenic 50.0%
CATTCCCATCTGCTTACTGG+TGG + chr7_3:78435001-78435020 Msa1126880:three_prime_UTR 50.0%
TTCCCATCTGCTTACTGGTG+GGG + chr7_3:78435003-78435022 Msa1126880:three_prime_UTR 50.0%
! GGAAGTGTGAACCCCTTACG+TGG + chr7_3:78434478-78434497 Msa1126880:CDS 55.0%
TGCGTCTGGGAACCACGTAA+GGG - chr7_3:78434493-78434512 None:intergenic 55.0%
TTGCGTCTGGGAACCACGTA+AGG - chr7_3:78434494-78434513 None:intergenic 55.0%
GTGGTTCCCAGACGCAATCT+GGG + chr7_3:78434497-78434516 Msa1126880:CDS 55.0%
TCCCAGACGCAATCTGGGTT+AGG + chr7_3:78434502-78434521 Msa1126880:CDS 55.0%
GTCCCCACCAGTAAGCAGAT+GGG - chr7_3:78435008-78435027 None:intergenic 55.0%
AGTCCCCACCAGTAAGCAGA+TGG - chr7_3:78435009-78435028 None:intergenic 55.0%
! GGAAGTGTGAACCCCTTACG+TGG + chr7_3:78434478-78434497 Msa1126880:CDS 55.0%
TGCGTCTGGGAACCACGTAA+GGG - chr7_3:78434493-78434512 None:intergenic 55.0%
TTGCGTCTGGGAACCACGTA+AGG - chr7_3:78434494-78434513 None:intergenic 55.0%
GTGGTTCCCAGACGCAATCT+GGG + chr7_3:78434497-78434516 Msa1126880:CDS 55.0%
TCCCAGACGCAATCTGGGTT+AGG + chr7_3:78434502-78434521 Msa1126880:CDS 55.0%
GTCCCCACCAGTAAGCAGAT+GGG - chr7_3:78435008-78435027 None:intergenic 55.0%
AGTCCCCACCAGTAAGCAGA+TGG - chr7_3:78435009-78435028 None:intergenic 55.0%
GCGTCTGGGAACCACGTAAG+GGG - chr7_3:78434492-78434511 None:intergenic 60.0%
CGTGGTTCCCAGACGCAATC+TGG + chr7_3:78434496-78434515 Msa1126880:CDS 60.0%
GCGTCTGGGAACCACGTAAG+GGG - chr7_3:78434492-78434511 None:intergenic 60.0%
CGTGGTTCCCAGACGCAATC+TGG + chr7_3:78434496-78434515 Msa1126880:CDS 60.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 78434219 78435110 78434219 ID=Msa1126880;Name=Msa1126880
chr7_3 mRNA 78434219 78435110 78434219 ID=Msa1126880-mRNA-1;Parent=Msa1126880;Name=Msa1126880-mRNA-1;_AED=0.13;_eAED=0.18;_QI=0|0|0.33|1|0.5|0.33|3|281|132
chr7_3 exon 78434219 78434311 78434219 ID=Msa1126880-mRNA-1:exon:21893;Parent=Msa1126880-mRNA-1
chr7_3 exon 78434420 78434568 78434420 ID=Msa1126880-mRNA-1:exon:21894;Parent=Msa1126880-mRNA-1
chr7_3 exon 78434673 78435110 78434673 ID=Msa1126880-mRNA-1:exon:21895;Parent=Msa1126880-mRNA-1
chr7_3 CDS 78434219 78434311 78434219 ID=Msa1126880-mRNA-1:cds;Parent=Msa1126880-mRNA-1
chr7_3 CDS 78434420 78434568 78434420 ID=Msa1126880-mRNA-1:cds;Parent=Msa1126880-mRNA-1
chr7_3 CDS 78434673 78434829 78434673 ID=Msa1126880-mRNA-1:cds;Parent=Msa1126880-mRNA-1
chr7_3 three_prime_UTR 78434830 78435110 78434830 ID=Msa1126880-mRNA-1:three_prime_utr;Parent=Msa1126880-mRNA-1
Gene Sequence

>Msa1126880

TTAGAGACTTTGAAAACAATATCAACACATGAGAATTTGCAAGGACTTGTGGATATTATTCTAGTCACTTCGAATACACTGATGAATGAAATGGTGGTTAAACGAGTCTTTGGCACATTACGTTTGTATGTTGTTGCAAATGAGAATCTATGGAAGTGTGAACCCCTTACGTGGTTCCCAGACGCAATCTGGGTTAGGATAGAGCAATTCCTTGCTTCATTTATCGGCAATTTTGGCGTCTTATATGAAGCTTCATTAATTCTAAAGAGATTGTGTCTTGAAGAGCTTGTGTTGGGTGATGTACTTAAGATTTTGGAGATTATAATCACAATCAAGAAATGGATTATACCTTGTCATTCTAGATGGCAGCCGATTACCATTTTCACTTACAGAGGCTAA

Protein sequence

>Msa1126880

LETLKTISTHENLQGLVDIILVTSNTLMNEMVVKRVFGTLRLYVVANENLWKCEPLTWFPDAIWVRIEQFLASFIGNFGVLYEASLILKRLCLEELVLGDVLKILEIIITIKKWIIPCHSRWQPITIFTYRG*