Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0346700 | XP_003611121.1 | 92.929 | 99 | 7 | 0 | 1 | 99 | 111 | 209 | 1.10e-56 | 184 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0346700 | sp|Q9FFA5|RMR14_ARATH | 57.292 | 96 | 41 | 0 | 2 | 97 | 107 | 202 | 6.78e-32 | 113 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0346700 | G7KDE4 | 92.929 | 99 | 7 | 0 | 1 | 99 | 111 | 209 | 5.25e-57 | 184 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0003400 | Msa0346700 | 0.807466 | 4.985711e-50 | -8.615850e-47 |
| Msa0005270 | Msa0346700 | 0.836129 | 1.121501e-56 | -8.615850e-47 |
| Msa0027890 | Msa0346700 | 0.816123 | 6.502738e-52 | -8.615850e-47 |
| Msa0031180 | Msa0346700 | 0.801745 | 7.788487e-49 | -8.615850e-47 |
| Msa0034350 | Msa0346700 | 0.806065 | 9.855990e-50 | -8.615850e-47 |
| Msa0042550 | Msa0346700 | 0.809807 | 1.576598e-50 | -8.615850e-47 |
| Msa0048000 | Msa0346700 | 0.801293 | 9.639692e-49 | -8.615850e-47 |
| Msa0050820 | Msa0346700 | 0.817094 | 3.941200e-52 | -8.615850e-47 |
| Msa0059890 | Msa0346700 | 0.837837 | 4.106597e-57 | -8.615850e-47 |
| Msa0078480 | Msa0346700 | 0.805850 | 1.094066e-49 | -8.615850e-47 |
| Msa0079110 | Msa0346700 | 0.800840 | 1.193198e-48 | -8.615850e-47 |
| Msa0085370 | Msa0346700 | 0.801243 | 9.871187e-49 | -8.615850e-47 |
| Msa0100160 | Msa0346700 | 0.801126 | 1.043069e-48 | -8.615850e-47 |
| Msa0104800 | Msa0346700 | 0.817653 | 2.950067e-52 | -8.615850e-47 |
| Msa0112750 | Msa0346700 | 0.826861 | 2.149942e-54 | -8.615850e-47 |
| Msa0140860 | Msa0346700 | 0.819144 | 1.355245e-52 | -8.615850e-47 |
| Msa0143850 | Msa0346700 | 0.813491 | 2.491719e-51 | -8.615850e-47 |
| Msa0146540 | Msa0346700 | 0.806026 | 1.004382e-49 | -8.615850e-47 |
| Msa0151180 | Msa0346700 | 0.807191 | 5.701472e-50 | -8.615850e-47 |
| Msa0154440 | Msa0346700 | 0.803120 | 4.057329e-49 | -8.615850e-47 |
| Msa0178510 | Msa0346700 | 0.822741 | 2.013702e-53 | -8.615850e-47 |
| Msa0185510 | Msa0346700 | 0.807928 | 3.977994e-50 | -8.615850e-47 |
| Msa0200560 | Msa0346700 | 0.811926 | 5.482817e-51 | -8.615850e-47 |
| Msa0205010 | Msa0346700 | 0.800946 | 1.135095e-48 | -8.615850e-47 |
| Msa0212040 | Msa0346700 | 0.806703 | 7.230512e-50 | -8.615850e-47 |
| Msa0225220 | Msa0346700 | 0.819147 | 1.352931e-52 | -8.615850e-47 |
| Msa0225640 | Msa0346700 | 0.807031 | 6.166184e-50 | -8.615850e-47 |
| Msa0243250 | Msa0346700 | 0.807371 | 5.222448e-50 | -8.615850e-47 |
| Msa0252540 | Msa0346700 | 0.801975 | 6.985981e-49 | -8.615850e-47 |
| Msa0264780 | Msa0346700 | 0.810067 | 1.385794e-50 | -8.615850e-47 |
| Msa0276920 | Msa0346700 | 0.801403 | 9.152631e-49 | -8.615850e-47 |
| Msa0281640 | Msa0346700 | 0.811823 | 5.774653e-51 | -8.615850e-47 |
| Msa0286670 | Msa0346700 | 0.801605 | 8.321663e-49 | -8.615850e-47 |
| Msa0320800 | Msa0346700 | 0.805769 | 1.137400e-49 | -8.615850e-47 |
| Msa0326520 | Msa0346700 | 0.818051 | 2.398422e-52 | -8.615850e-47 |
| Msa0342010 | Msa0346700 | 0.827438 | 1.564796e-54 | -8.615850e-47 |
| Msa0346700 | Msa0389910 | 0.812869 | 3.411872e-51 | -8.615850e-47 |
| Msa0346700 | Msa0411410 | 0.811274 | 7.598367e-51 | -8.615850e-47 |
| Msa0346700 | Msa0432500 | 0.800870 | 1.176519e-48 | -8.615850e-47 |
| Msa0346700 | Msa0433410 | -0.803929 | 2.756428e-49 | -8.615850e-47 |
| Msa0346700 | Msa0437400 | 0.807104 | 5.948205e-50 | -8.615850e-47 |
| Msa0346700 | Msa0443000 | 0.810701 | 1.011498e-50 | -8.615850e-47 |
| Msa0346700 | Msa0463550 | 0.807447 | 5.032236e-50 | -8.615850e-47 |
| Msa0346700 | Msa0484780 | 0.846696 | 1.846885e-59 | -8.615850e-47 |
| Msa0346700 | Msa0506830 | 0.851939 | 6.402297e-61 | -8.615850e-47 |
| Msa0346700 | Msa0512900 | 0.809513 | 1.823003e-50 | -8.615850e-47 |
| Msa0346700 | Msa0530650 | 0.805843 | 1.097771e-49 | -8.615850e-47 |
| Msa0346700 | Msa0565940 | 0.807352 | 5.270695e-50 | -8.615850e-47 |
| Msa0346700 | Msa0571780 | 0.800003 | 1.767165e-48 | -8.615850e-47 |
| Msa0346700 | Msa0574390 | 0.815292 | 9.964472e-52 | -8.615850e-47 |
| Msa0346700 | Msa0576680 | 0.805458 | 1.322261e-49 | -8.615850e-47 |
| Msa0346700 | Msa0581160 | 0.809446 | 1.884692e-50 | -8.615850e-47 |
| Msa0346700 | Msa0600080 | 0.809691 | 1.669935e-50 | -8.615850e-47 |
| Msa0346700 | Msa0641730 | 0.813984 | 1.940292e-51 | -8.615850e-47 |
| Msa0346700 | Msa0693380 | 0.824790 | 6.669263e-54 | -8.615850e-47 |
| Msa0346700 | Msa0704920 | 0.806466 | 8.114340e-50 | -8.615850e-47 |
| Msa0346700 | Msa0711830 | 0.815185 | 1.052340e-51 | -8.615850e-47 |
| Msa0346700 | Msa0721280 | 0.878808 | 2.080773e-69 | -8.615850e-47 |
| Msa0346700 | Msa0750240 | 0.812581 | 3.945954e-51 | -8.615850e-47 |
| Msa0346700 | Msa0753370 | 0.804912 | 1.719709e-49 | -8.615850e-47 |
| Msa0346700 | Msa0763890 | 0.810557 | 1.086433e-50 | -8.615850e-47 |
| Msa0346700 | Msa0763920 | 0.806530 | 7.866117e-50 | -8.615850e-47 |
| Msa0346700 | Msa0773590 | 0.821485 | 3.937494e-53 | -8.615850e-47 |
| Msa0346700 | Msa0784210 | 0.804806 | 1.810410e-49 | -8.615850e-47 |
| Msa0346700 | Msa0794700 | 0.815047 | 1.129085e-51 | -8.615850e-47 |
| Msa0346700 | Msa0795360 | 0.803646 | 3.157154e-49 | -8.615850e-47 |
| Msa0346700 | Msa0795370 | 0.842608 | 2.330498e-58 | -8.615850e-47 |
| Msa0346700 | Msa0800700 | 0.812662 | 3.786851e-51 | -8.615850e-47 |
| Msa0346700 | Msa0811040 | 0.817369 | 3.418092e-52 | -8.615850e-47 |
| Msa0346700 | Msa0817460 | 0.806019 | 1.007834e-49 | -8.615850e-47 |
| Msa0346700 | Msa0830510 | 0.829760 | 4.298895e-55 | -8.615850e-47 |
| Msa0346700 | Msa0853950 | 0.812442 | 4.231371e-51 | -8.615850e-47 |
| Msa0346700 | Msa0866400 | 0.800898 | 1.161072e-48 | -8.615850e-47 |
| Msa0346700 | Msa0866410 | 0.801248 | 9.847802e-49 | -8.615850e-47 |
| Msa0346700 | Msa0873860 | 0.802134 | 6.478621e-49 | -8.615850e-47 |
| Msa0346700 | Msa0898750 | 0.802689 | 4.978843e-49 | -8.615850e-47 |
| Msa0346700 | Msa0900620 | 0.804777 | 1.835310e-49 | -8.615850e-47 |
| Msa0346700 | Msa0928130 | 0.805224 | 1.479757e-49 | -8.615850e-47 |
| Msa0346700 | Msa0939000 | 0.804226 | 2.391312e-49 | -8.615850e-47 |
| Msa0346700 | Msa0962840 | 0.824047 | 9.975140e-54 | -8.615850e-47 |
| Msa0346700 | Msa0964310 | 0.812533 | 4.042049e-51 | -8.615850e-47 |
| Msa0346700 | Msa0975940 | 0.807551 | 4.783716e-50 | -8.615850e-47 |
| Msa0346700 | Msa0986510 | 0.806421 | 8.295804e-50 | -8.615850e-47 |
| Msa0346700 | Msa1031930 | 0.839211 | 1.814736e-57 | -8.615850e-47 |
| Msa0346700 | Msa1032170 | 0.833967 | 3.933662e-56 | -8.615850e-47 |
| Msa0346700 | Msa1063420 | 0.816793 | 4.605320e-52 | -8.615850e-47 |
| Msa0346700 | Msa1071770 | 0.815189 | 1.050162e-51 | -8.615850e-47 |
| Msa0346700 | Msa1082500 | 0.828228 | 1.010086e-54 | -8.615850e-47 |
| Msa0346700 | Msa1087980 | 0.807906 | 4.020820e-50 | -8.615850e-47 |
| Msa0346700 | Msa1105500 | 0.800603 | 1.333610e-48 | -8.615850e-47 |
| Msa0346700 | Msa1126880 | 0.804554 | 2.043571e-49 | -8.615850e-47 |
| Msa0346700 | Msa1126950 | 0.802043 | 6.764930e-49 | -8.615850e-47 |
| Msa0346700 | Msa1152510 | 0.800609 | 1.329766e-48 | -8.615850e-47 |
| Msa0346700 | Msa1158660 | 0.804466 | 2.131757e-49 | -8.615850e-47 |
| Msa0346700 | Msa1158830 | 0.822870 | 1.879178e-53 | -8.615850e-47 |
| Msa0346700 | Msa1174850 | 0.801866 | 7.355061e-49 | -8.615850e-47 |
| Msa0346700 | Msa1205370 | 0.820869 | 5.461306e-53 | -8.615850e-47 |
| Msa0346700 | Msa1227150 | 0.819477 | 1.137754e-52 | -8.615850e-47 |
| Msa0346700 | Msa1250290 | 0.817695 | 2.885974e-52 | -8.615850e-47 |
| Msa0346700 | Msa1270660 | 0.809691 | 1.669253e-50 | -8.615850e-47 |
| Msa0346700 | Msa1270730 | 0.809882 | 1.518658e-50 | -8.615850e-47 |
| Msa0346700 | Msa1340260 | 0.804816 | 1.801554e-49 | -8.615850e-47 |
| Msa0346700 | Msa1348880 | 0.810396 | 1.177045e-50 | -8.615850e-47 |
| Msa0346700 | Msa1353470 | 0.820466 | 6.757567e-53 | -8.615850e-47 |
| Msa0346700 | Msa1357890 | 0.876116 | 1.802416e-68 | -8.615850e-47 |
| Msa0346700 | Msa1372150 | 0.830504 | 2.828769e-55 | -8.615850e-47 |
| Msa0346700 | Msa1392700 | 0.823742 | 1.175773e-53 | -8.615850e-47 |
| Msa0346700 | Msa1398090 | 0.871022 | 9.376853e-67 | -8.615850e-47 |
| Msa0346700 | Msa1410210 | 0.811571 | 6.550864e-51 | -8.615850e-47 |
| Msa0346700 | Msa1440940 | 0.807973 | 3.891433e-50 | -8.615850e-47 |
| Msa0346700 | Msa1457430 | 0.830458 | 2.903175e-55 | -8.615850e-47 |
| Msa0346700 | Msa1462290 | 0.815243 | 1.021746e-51 | -8.615850e-47 |
| Msa0346700 | Msa1463900 | 0.818766 | 1.651585e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0346700 | MtrunA17_Chr5g0397781 | 92.929 | 99 | 7 | 0 | 1 | 99 | 111 | 209 | 1.01e-60 | 184 |
| Msa0346700 | MtrunA17_Chr8g0386521 | 59.184 | 98 | 40 | 0 | 2 | 99 | 103 | 200 | 1.59e-35 | 120 |
| Msa0346700 | MtrunA17_Chr8g0349271 | 55.208 | 96 | 43 | 0 | 2 | 97 | 105 | 200 | 2.93e-29 | 103 |
| Msa0346700 | MtrunA17_Chr1g0203401 | 51.042 | 96 | 47 | 0 | 2 | 97 | 114 | 209 | 2.55e-27 | 99.0 |
| Msa0346700 | MtrunA17_Chr7g0247121 | 52.308 | 65 | 31 | 0 | 33 | 97 | 34 | 98 | 1.55e-17 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0346700 | AT5G23750.2 | 57.292 | 96 | 41 | 0 | 2 | 97 | 106 | 201 | 6.03e-33 | 113 |
| Msa0346700 | AT5G23750.1 | 57.292 | 96 | 41 | 0 | 2 | 97 | 107 | 202 | 6.89e-33 | 113 |
| Msa0346700 | AT5G23750.3 | 57.292 | 96 | 41 | 0 | 2 | 97 | 134 | 229 | 8.29e-33 | 114 |
| Msa0346700 | AT3G48940.1 | 56.250 | 96 | 42 | 0 | 2 | 97 | 80 | 175 | 1.49e-30 | 106 |
| Msa0346700 | AT3G61260.1 | 53.125 | 96 | 45 | 0 | 2 | 97 | 117 | 212 | 1.52e-28 | 102 |
| Msa0346700 | AT2G45820.1 | 57.292 | 96 | 41 | 0 | 2 | 97 | 95 | 190 | 1.41e-23 | 89.4 |
| Msa0346700 | AT4G00670.1 | 35.052 | 97 | 62 | 1 | 2 | 97 | 27 | 123 | 8.28e-14 | 62.8 |
| Msa0346700 | AT1G63295.1 | 37.895 | 95 | 56 | 2 | 6 | 97 | 57 | 151 | 2.80e-11 | 56.6 |
| Msa0346700 | AT1G63295.2 | 37.895 | 95 | 56 | 2 | 6 | 97 | 97 | 191 | 5.28e-11 | 56.6 |
Find 13 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCAATCTTCTGTTGGATTA+TGG | 0.203365 | 3_1:-7350543 | Msa0346700:CDS |
| AAGCATGCAAACAATTTCTT+TGG | 0.279239 | 3_1:+7349987 | None:intergenic |
| GCAATCTTCTGTTGGATTAT+GGG | 0.326593 | 3_1:-7350542 | Msa0346700:CDS |
| GAGCTTGATGAAAGATGAAA+TGG | 0.419331 | 3_1:-7350585 | None:intergenic |
| GGAAGAATGTCTTGAGGTAG+AGG | 0.455749 | 3_1:-7350045 | Msa0346700:CDS |
| TACAAGAAGCAATCTTCTGT+TGG | 0.476983 | 3_1:-7350550 | Msa0346700:CDS |
| AAGCAAGAAATCATCTATAG+AGG | 0.477360 | 3_1:-7350515 | Msa0346700:CDS |
| GGCACAACTCAAGAAATTTG+AGG | 0.555597 | 3_1:-7350494 | Msa0346700:intron |
| GAAAGATGAAATGGTCACGC+AGG | 0.578055 | 3_1:-7350576 | None:intergenic |
| ATTTCTTTGGTGCAACTCCA+CGG | 0.591194 | 3_1:+7350000 | None:intergenic |
| GCTGCAAAGTTTCGTAGCCG+TGG | 0.595886 | 3_1:-7350017 | Msa0346700:CDS |
| ATCTTCTGTTGGATTATGGG+AGG | 0.596122 | 3_1:-7350539 | Msa0346700:CDS |
| AAAGATGAAATGGTCACGCA+GGG | 0.637735 | 3_1:-7350575 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGATTATTAGTTATTATTTA+TGG | - | chr3_1:7350077-7350096 | Msa0346700:CDS | 10.0% |
| !!! | ACAAAATTGTTTTAATTAAT+AGG | - | chr3_1:7350371-7350390 | Msa0346700:intron | 10.0% |
| !!! | TTTTAATTAATAGGAAAATT+TGG | - | chr3_1:7350380-7350399 | Msa0346700:intron | 10.0% |
| !!! | GTTTTGTTTTGTTGTAAATT+AGG | - | chr3_1:7350116-7350135 | Msa0346700:CDS | 20.0% |
| !!! | ATTTTGTAAGTTACAAATGA+GGG | + | chr3_1:7350358-7350377 | None:intergenic | 20.0% |
| !!! | AATTTTGTAAGTTACAAATG+AGG | + | chr3_1:7350359-7350378 | None:intergenic | 20.0% |
| !! | ATGAATAAAGTGAGTATTGT+GGG | + | chr3_1:7350298-7350317 | None:intergenic | 25.0% |
| ! | AATAGTTGAAGCTCAAAAAA+GGG | - | chr3_1:7350482-7350501 | Msa0346700:intron | 25.0% |
| AAGCAAGAAATCATCTATAG+AGG | - | chr3_1:7350033-7350052 | Msa0346700:CDS | 30.0% | |
| TCTAGTATAACCAAGGTTTA+GGG | + | chr3_1:7350239-7350258 | None:intergenic | 30.0% | |
| TTCTAGTATAACCAAGGTTT+AGG | + | chr3_1:7350240-7350259 | None:intergenic | 30.0% | |
| ! | GATGAATAAAGTGAGTATTG+TGG | + | chr3_1:7350299-7350318 | None:intergenic | 30.0% |
| CAATAGTTGAAGCTCAAAAA+AGG | - | chr3_1:7350481-7350500 | Msa0346700:intron | 30.0% | |
| AAGCATGCAAACAATTTCTT+TGG | + | chr3_1:7350564-7350583 | None:intergenic | 30.0% | |
| TACAAGAAGCAATCTTCTGT+TGG | - | chr3_1:7349998-7350017 | Msa0346700:CDS | 35.0% | |
| AGCAATCTTCTGTTGGATTA+TGG | - | chr3_1:7350005-7350024 | Msa0346700:CDS | 35.0% | |
| GCAATCTTCTGTTGGATTAT+GGG | - | chr3_1:7350006-7350025 | Msa0346700:CDS | 35.0% | |
| CATCTCTTCTAGTATAACCA+AGG | + | chr3_1:7350246-7350265 | None:intergenic | 35.0% | |
| TGGTTATACTAGAAGAGATG+CGG | - | chr3_1:7350246-7350265 | Msa0346700:intron | 35.0% | |
| AAAAAGGGAAGAATGTCTTG+AGG | - | chr3_1:7350497-7350516 | Msa0346700:CDS | 35.0% | |
| ATCTTCTGTTGGATTATGGG+AGG | - | chr3_1:7350009-7350028 | Msa0346700:CDS | 40.0% | |
| GGCACAACTCAAGAAATTTG+AGG | - | chr3_1:7350054-7350073 | Msa0346700:CDS | 40.0% | |
| GGTTATACTAGAAGAGATGC+GGG | - | chr3_1:7350247-7350266 | Msa0346700:intron | 40.0% | |
| TCATCTCTTCTAAGCACTCT+TGG | - | chr3_1:7350270-7350289 | Msa0346700:intron | 40.0% | |
| CATCTCTTCTAAGCACTCTT+GGG | - | chr3_1:7350271-7350290 | Msa0346700:intron | 40.0% | |
| ! | ATTTCTTTGGTGCAACTCCA+CGG | + | chr3_1:7350551-7350570 | None:intergenic | 40.0% |
| CGACACATTTCAGTTCCACA+AGG | + | chr3_1:7350163-7350182 | None:intergenic | 45.0% | |
| CGAACTTGTGCATGTAACAG+AGG | + | chr3_1:7350208-7350227 | None:intergenic | 45.0% | |
| GGAAGAATGTCTTGAGGTAG+AGG | - | chr3_1:7350503-7350522 | Msa0346700:CDS | 45.0% | |
| CGAGATTGAGCCCTAAACCT+TGG | - | chr3_1:7350226-7350245 | Msa0346700:intron | 50.0% | |
| ! | TGCGTGCAAGTGTAGCCTTG+TGG | - | chr3_1:7350145-7350164 | Msa0346700:CDS | 55.0% |
| GCTGCAAAGTTTCGTAGCCG+TGG | - | chr3_1:7350531-7350550 | Msa0346700:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_1 | gene | 7349977 | 7350593 | 7349977 | ID=Msa0346700;Name=Msa0346700 |
| chr3_1 | mRNA | 7349977 | 7350593 | 7349977 | ID=Msa0346700-mRNA-1;Parent=Msa0346700;Name=Msa0346700-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0|0|1|1|1|2|0|99 |
| chr3_1 | exon | 7350495 | 7350593 | 7350495 | ID=Msa0346700-mRNA-1:exon:2086;Parent=Msa0346700-mRNA-1 |
| chr3_1 | exon | 7349977 | 7350177 | 7349977 | ID=Msa0346700-mRNA-1:exon:2085;Parent=Msa0346700-mRNA-1 |
| chr3_1 | CDS | 7350495 | 7350593 | 7350495 | ID=Msa0346700-mRNA-1:cds;Parent=Msa0346700-mRNA-1 |
| chr3_1 | CDS | 7349977 | 7350177 | 7349977 | ID=Msa0346700-mRNA-1:cds;Parent=Msa0346700-mRNA-1 |
| Gene Sequence |
| Protein sequence |
