AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1261160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1261160 MtrunA17_Chr4g0062841 98.276 290 5 0 1 290 1 290 0.0 573
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1261160 AT5G19930.1 66.316 285 96 0 6 290 4 288 8.77e-133 378
Msa1261160 AT5G19930.2 66.316 285 96 0 6 290 4 288 8.77e-133 378

Find 93 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTTCTGATTGTAGCTTTA+TGG 0.071257 8_2:-63475630 Msa1261160:CDS
AAAGAGAAGAATAATAATTT+TGG 0.190946 8_2:-63477070 Msa1261160:five_prime_UTR
TTTGAGTGAGTTTCCGAAAT+TGG 0.205361 8_2:+63476841 None:intergenic
AAAATTATTATTCTTCTCTT+TGG 0.231956 8_2:+63477072 None:intergenic
TGTCCACCTTGTTTGAATTC+AGG 0.232979 8_2:+63476482 None:intergenic
TGTTCTGATTGTAGCTTTAT+GGG 0.234865 8_2:-63475629 Msa1261160:CDS
GCTCTCATTGGTGGTGTTAT+TGG 0.255394 8_2:-63475550 Msa1261160:CDS
TCAGTTCTTGTATCCATATC+TGG 0.264005 8_2:-63474117 Msa1261160:three_prime_UTR
CGTGTTCCTCGTTGCCTATT+CGG 0.276192 8_2:-63477045 Msa1261160:five_prime_UTR
AAAGGAGGAAGCCAAATAAA+AGG 0.279137 8_2:-63474160 Msa1261160:three_prime_UTR
TTCTAGTTATAATATAATAT+TGG 0.287067 8_2:+63477010 None:intergenic
AGCACTTCTGGTGCACTTGC+TGG 0.299865 8_2:-63476726 Msa1261160:CDS
GTGAGCTCTGAATGCGATTA+AGG 0.301312 8_2:+63476764 None:intergenic
TGATTATGCATGGCTTTGTT+AGG 0.344707 8_2:-63473985 Msa1261160:three_prime_UTR
CCCTCTATGTTGTAGGTTGT+TGG 0.355656 8_2:-63474403 Msa1261160:intron
TCATTTCTGAAACCCAATTT+CGG 0.364843 8_2:-63476854 Msa1261160:five_prime_UTR
TCCTCGTTGCCTATTCGGTT+TGG 0.372588 8_2:-63477040 Msa1261160:five_prime_UTR
CGGATCCACTGAGAAATAGA+TGG 0.376312 8_2:+63476813 None:intergenic
CTCCATTGCAGCAGCAATAA+TGG 0.378209 8_2:+63475527 None:intergenic
TTGAGTGAGTTTCCGAAATT+GGG 0.382190 8_2:+63476842 None:intergenic
GGCGCAACATTGCAATTTAG+TGG 0.386948 8_2:-63474538 Msa1261160:CDS
TCAAAGACTTGCTTGATAAG+AGG 0.393314 8_2:+63474182 None:intergenic
GGTTGCATTCCAAACCGAAT+AGG 0.409105 8_2:+63477031 None:intergenic
AACAGAGGCAATGCCACTAT+TGG 0.410928 8_2:+63475648 None:intergenic
AAGTATCATTGACTCTCTAT+TGG 0.411402 8_2:-63474560 Msa1261160:CDS
AACAAGGTGGACAAAGAAAC+TGG 0.420356 8_2:-63476472 Msa1261160:intron
TAGAATTCTCAAGCAACTAT+TGG 0.422184 8_2:-63474617 Msa1261160:CDS
AAAGAGGTTATTCACCTTGA+AGG 0.422918 8_2:+63475447 None:intergenic
TGTAATCGAATGATAGAATA+GGG 0.428013 8_2:-63474208 Msa1261160:three_prime_UTR
TAGAGCAGAATAATAAAGTA+TGG 0.431621 8_2:-63477109 None:intergenic
TAATAACATGTGAGTTTGGT+TGG 0.437158 8_2:+63476961 None:intergenic
GCCAAATAAAAGGGCGGAAA+GGG 0.442458 8_2:-63474150 Msa1261160:three_prime_UTR
GACACATGGTCTTCTGAGCT+TGG 0.442992 8_2:-63475505 Msa1261160:CDS
TTGTAATCGAATGATAGAAT+AGG 0.444371 8_2:-63474209 Msa1261160:three_prime_UTR
TTGTAGGTTGTTGGAAATCC+AGG 0.453453 8_2:-63474394 Msa1261160:intron
CCACTCCACCTCGTGGTAGT+TGG 0.459095 8_2:-63476932 Msa1261160:five_prime_UTR
AGTATCATTGACTCTCTATT+GGG 0.459444 8_2:-63474559 Msa1261160:CDS
ATAGAACTAGAAACCAGATA+TGG 0.463981 8_2:+63474104 None:intergenic
ATTTCGGAAACTCACTCAAA+TGG 0.468503 8_2:-63476838 Msa1261160:exon
TTCTCAGTGGATCCGACCAT+TGG 0.469540 8_2:-63476805 Msa1261160:CDS
GCAGCTGGTGGAAGTGTCAT+TGG 0.473800 8_2:-63474688 Msa1261160:CDS
TCGTCTAATCACAACCTTCA+AGG 0.476556 8_2:-63475461 Msa1261160:intron
GCTCTAATTACCGCTCTCAT+TGG 0.488116 8_2:-63475562 Msa1261160:CDS
GGACTTCTAGCAGCTGCAGC+TGG 0.498554 8_2:-63474703 Msa1261160:CDS
CCATTGGTTGCTGTCACTGT+TGG 0.504140 8_2:-63476789 Msa1261160:CDS
GATACAAGAACTGAATCAAA+AGG 0.504443 8_2:+63474126 None:intergenic
ATACCCCTCACTACCGTGGC+AGG 0.517394 8_2:-63474592 Msa1261160:CDS
ATACAAGTTCTGGCCAATAG+TGG 0.522110 8_2:-63475661 Msa1261160:CDS
AGCAATTGACGTTAAAATTG+TGG 0.527955 8_2:+63474297 None:intergenic
CCTGTGAGGAAGGGTACAAA+TGG 0.528132 8_2:-63474742 Msa1261160:CDS
CTAATTACCGCTCTCATTGG+TGG 0.531975 8_2:-63475559 Msa1261160:CDS
GGCCATTATTGCTGCTGCAA+TGG 0.538240 8_2:-63475529 Msa1261160:CDS
CCATTTGTACCCTTCCTCAC+AGG 0.544901 8_2:+63474742 None:intergenic
TTATCAAGCAAGTCTTTGAA+AGG 0.549680 8_2:-63474178 Msa1261160:three_prime_UTR
CTTCTAGCAGCTGCAGCTGG+TGG 0.554176 8_2:-63474700 Msa1261160:CDS
AGCCAAATAAAAGGGCGGAA+AGG 0.558019 8_2:-63474151 Msa1261160:three_prime_UTR
GCTTTATGGGTACTAACAGA+AGG 0.570914 8_2:-63475616 Msa1261160:CDS
ATAGTCCTGCCACGGTAGTG+AGG 0.574585 8_2:+63474587 None:intergenic
AAGGAGGAAGCCAAATAAAA+GGG 0.578629 8_2:-63474159 Msa1261160:three_prime_UTR
TCAAGCAAGTCTTTGAAAGG+AGG 0.586218 8_2:-63474175 Msa1261160:three_prime_UTR
ACTACCGTGGCAGGACTATG+TGG 0.586247 8_2:-63474583 Msa1261160:CDS
TTGGAGTTCTCAGTAATGAT+CGG 0.586787 8_2:-63475486 Msa1261160:CDS
ATGGGTACTAACAGAAGGGA+AGG 0.594387 8_2:-63475611 Msa1261160:CDS
TAGTCCTGCCACGGTAGTGA+GGG 0.595711 8_2:+63474588 None:intergenic
TAATCGCATTCAGAGCTCAC+AGG 0.596274 8_2:-63476761 Msa1261160:CDS
CCAACTACCACGAGGTGGAG+TGG 0.600830 8_2:+63476932 None:intergenic
ATTCTGCTCTATTCGCCAGA+AGG 0.615062 8_2:-63474515 Msa1261160:intron
GTGCATGAACTCTAAAGACT+CGG 0.615281 8_2:-63475584 Msa1261160:CDS
CAAGTGCACCAGAAGTGCTG+AGG 0.620284 8_2:+63476730 None:intergenic
GGATCCACTGAGAAATAGAT+GGG 0.623325 8_2:+63476814 None:intergenic
AAGTGCACCAGAAGTGCTGA+GGG 0.630378 8_2:+63476731 None:intergenic
CTTTATGGGTACTAACAGAA+GGG 0.631113 8_2:-63475615 Msa1261160:CDS
ATGAAGAGGATGATTATGCA+TGG 0.634894 8_2:-63473995 Msa1261160:three_prime_UTR
TTGAAGGTTGTGATTAGACG+AGG 0.644573 8_2:+63475463 None:intergenic
ATTGATCCTGAATTCAAACA+AGG 0.648969 8_2:-63476488 Msa1261160:CDS
CAGTGACAGCAACCAATGGT+CGG 0.652095 8_2:+63476793 None:intergenic
CCAACAGTGACAGCAACCAA+TGG 0.662526 8_2:+63476789 None:intergenic
AGTCCTGCCACGGTAGTGAG+GGG 0.669040 8_2:+63474589 None:intergenic
GAGGAAGCCAAATAAAAGGG+CGG 0.673162 8_2:-63474156 Msa1261160:three_prime_UTR
ACTTCCACATAGTCCTGCCA+CGG 0.681568 8_2:+63474579 None:intergenic
AACAACCAACTACCACGAGG+TGG 0.685328 8_2:+63476927 None:intergenic
GATCCTGAATTCAAACAAGG+TGG 0.686429 8_2:-63476485 Msa1261160:CDS
AATAACACCACCAATGAGAG+CGG 0.688307 8_2:+63475552 None:intergenic
TAAAGCTACAATCAGAACAG+AGG 0.688389 8_2:+63475633 None:intergenic
AAAAGGGACAAGATACGAAG+CGG 0.694625 8_2:-63476514 Msa1261160:CDS
GGTCATACCCCTCACTACCG+TGG 0.698974 8_2:-63474596 Msa1261160:CDS
GTGAGGAAGGGTACAAATGG+TGG 0.703389 8_2:-63474739 Msa1261160:CDS
AAAAGTTCCACTCCACCTCG+TGG 0.705572 8_2:-63476939 Msa1261160:five_prime_UTR
TGGACCCATCTATTTCTCAG+TGG 0.712343 8_2:-63476818 Msa1261160:CDS
GCTGCTGCAATGGAGACACA+TGG 0.733987 8_2:-63475519 Msa1261160:CDS
AATCGAATGATAGAATAGGG+CGG 0.751980 8_2:-63474205 Msa1261160:three_prime_UTR
TCCAAACCGAATAGGCAACG+AGG 0.761278 8_2:+63477039 None:intergenic
AAGAACAACCAACTACCACG+AGG 0.800597 8_2:+63476924 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTAGTTATAATATAATAT+TGG + chr8_2:63474044-63474063 None:intergenic 10.0%
!! TTTATTATAATCAATATTAC+AGG - chr8_2:63475022-63475041 Msa1261160:intron 10.0%
!! AAAATTATTATTCTTCTCTT+TGG + chr8_2:63473982-63474001 None:intergenic 15.0%
!!! AAAGAGAAGAATAATAATTT+TGG - chr8_2:63473981-63474000 Msa1261160:three_prime_UTR 15.0%
!!! TTGTTTGTTTTTTTTTTATC+AGG + chr8_2:63474709-63474728 None:intergenic 15.0%
!!! TTCATGTTTTTATCTTAATA+AGG + chr8_2:63477041-63477060 None:intergenic 15.0%
!!! CAATCTTTTTTGTTGTTTAA+TGG - chr8_2:63474434-63474453 Msa1261160:intron 20.0%
!! AAAAGAAAAAGAGCAAAAAA+GGG + chr8_2:63474632-63474651 None:intergenic 20.0%
!! TAAAAGAAAAAGAGCAAAAA+AGG + chr8_2:63474633-63474652 None:intergenic 20.0%
!!! GAGTTTTTTTTACTATAAGA+TGG - chr8_2:63474830-63474849 Msa1261160:intron 20.0%
!!! TTTTTTGAAAATTCAGTATC+CGG + chr8_2:63474948-63474967 None:intergenic 20.0%
!! AACGCTAAAAATTCTAATTA+GGG - chr8_2:63475124-63475143 Msa1261160:intron 20.0%
!! TACATAAAAAAGCTAAAACT+TGG - chr8_2:63475201-63475220 Msa1261160:intron 20.0%
!!! TAATTGCAACAATTTTCTTT+TGG - chr8_2:63475254-63475273 Msa1261160:intron 20.0%
!! CTAATTAGTAAATGCAAATT+AGG + chr8_2:63475669-63475688 None:intergenic 20.0%
!!! CTAATTTGCATTTACTAATT+AGG - chr8_2:63475667-63475686 Msa1261160:intron 20.0%
!!! AATGTTTTTGACATTGTAAA+AGG + chr8_2:63476012-63476031 None:intergenic 20.0%
!! TACATTTGAACAAAATTCAA+TGG - chr8_2:63476598-63476617 Msa1261160:intron 20.0%
!!! GATTACAAATTTTGAATTCT+TGG + chr8_2:63476830-63476849 None:intergenic 20.0%
!! ATTAAGATAAAAACATGAAG+AGG - chr8_2:63477042-63477061 Msa1261160:five_prime_UTR 20.0%
!! CTTTTAATAACATGTGAGTT+TGG + chr8_2:63474097-63474116 None:intergenic 25.0%
!! TCATTCATTCATTTTCAACA+AGG - chr8_2:63474465-63474484 Msa1261160:intron 25.0%
! AAAAAAGGGAACAAAGAAAA+GGG + chr8_2:63474618-63474637 None:intergenic 25.0%
! TAGAATTGATTAAACTTGTG+CGG - chr8_2:63474752-63474771 Msa1261160:intron 25.0%
!! TATAACTTGAGGTTAGATTT+TGG - chr8_2:63474808-63474827 Msa1261160:intron 25.0%
! TTGGATACATTTATGTAAGT+GGG + chr8_2:63474867-63474886 None:intergenic 25.0%
! ACATAAATGTATCCAACAAT+TGG - chr8_2:63474871-63474890 Msa1261160:intron 25.0%
! GAACGCTAAAAATTCTAATT+AGG - chr8_2:63475123-63475142 Msa1261160:intron 25.0%
!!! ATGGTTTTTTTGTCACATTA+TGG - chr8_2:63475316-63475335 Msa1261160:intron 25.0%
! AAATGCAAATTAGGAATAGA+AGG + chr8_2:63475660-63475679 None:intergenic 25.0%
!!! TTGATGTTCATTAATTTGCT+AGG - chr8_2:63475735-63475754 Msa1261160:intron 25.0%
! AATATTAATCAAAACATCCC+TGG - chr8_2:63475770-63475789 Msa1261160:intron 25.0%
!! TCACAGCTTAAATATGTTTT+TGG - chr8_2:63475885-63475904 Msa1261160:intron 25.0%
! ATTTGTGGATCCTTTATTAA+GGG + chr8_2:63476075-63476094 None:intergenic 25.0%
! AATTTGTGGATCCTTTATTA+AGG + chr8_2:63476076-63476095 None:intergenic 25.0%
! CTTATAAGTTATAACATCTG+TGG - chr8_2:63476181-63476200 Msa1261160:intron 25.0%
! TTGTAATCGAATGATAGAAT+AGG - chr8_2:63476842-63476861 Msa1261160:five_prime_UTR 25.0%
! TGTAATCGAATGATAGAATA+GGG - chr8_2:63476843-63476862 Msa1261160:five_prime_UTR 25.0%
!! AGCATCAGAATGATATTTTT+GGG - chr8_2:63477003-63477022 Msa1261160:intron 25.0%
TAATAACATGTGAGTTTGGT+TGG + chr8_2:63474093-63474112 None:intergenic 30.0%
!! TCATTTCTGAAACCCAATTT+CGG - chr8_2:63474197-63474216 Msa1261160:three_prime_UTR 30.0%
! TTCACGTTTTTGTTGGATTT+AGG - chr8_2:63474365-63474384 Msa1261160:CDS 30.0%
! TTCATTTTCAACAAGGTTTG+CGG - chr8_2:63474472-63474491 Msa1261160:intron 30.0%
ATTGATCCTGAATTCAAACA+AGG - chr8_2:63474563-63474582 Msa1261160:CDS 30.0%
CAAAAAAGGGAACAAAGAAA+AGG + chr8_2:63474619-63474638 None:intergenic 30.0%
ATGTAGTATAACTCAAGACA+AGG + chr8_2:63474656-63474675 None:intergenic 30.0%
CTACAATGGAATATAACTTG+AGG - chr8_2:63474797-63474816 Msa1261160:intron 30.0%
GTTGGATACATTTATGTAAG+TGG + chr8_2:63474868-63474887 None:intergenic 30.0%
!! TTTGGTGTTTAGTTTGAGTT+TGG - chr8_2:63475272-63475291 Msa1261160:intron 30.0%
!!! TTTTTTGTCACATTATGGTG+AGG - chr8_2:63475321-63475340 Msa1261160:intron 30.0%
GGGAAATTTATACCAAATTC+AGG + chr8_2:63475354-63475373 None:intergenic 30.0%
!!! ATTTCCCTACTGTTTTTTTC+AGG - chr8_2:63475367-63475386 Msa1261160:intron 30.0%
GTATCCTGAAAAAAACAGTA+GGG + chr8_2:63475374-63475393 None:intergenic 30.0%
TGTATCCTGAAAAAAACAGT+AGG + chr8_2:63475375-63475394 None:intergenic 30.0%
!!! TTTTTTCAGGATACAAGTTC+TGG - chr8_2:63475380-63475399 Msa1261160:intron 30.0%
!! TGTTCTGATTGTAGCTTTAT+GGG - chr8_2:63475422-63475441 Msa1261160:intron 30.0%
! ATGTTGTTGCTTAAACAAAG+AGG + chr8_2:63475623-63475642 None:intergenic 30.0%
! TTTCGTAATTTGTGATTGTG+TGG - chr8_2:63475939-63475958 Msa1261160:intron 30.0%
! TTCGTAATTTGTGATTGTGT+GGG - chr8_2:63475940-63475959 Msa1261160:intron 30.0%
!! GGAATGTTTTTGAGAATTTG+TGG + chr8_2:63476090-63476109 None:intergenic 30.0%
TATAACTTATAAGCCACTTG+TGG + chr8_2:63476174-63476193 None:intergenic 30.0%
!!! TTCTAGAACTTTTGACAATC+AGG - chr8_2:63476400-63476419 Msa1261160:intron 30.0%
TAGAATTCTCAAGCAACTAT+TGG - chr8_2:63476434-63476453 Msa1261160:intron 30.0%
!! AAGTATCATTGACTCTCTAT+TGG - chr8_2:63476491-63476510 Msa1261160:CDS 30.0%
!! AGTATCATTGACTCTCTATT+GGG - chr8_2:63476492-63476511 Msa1261160:CDS 30.0%
GTGTATCAAAATTTACCTTC+TGG + chr8_2:63476554-63476573 None:intergenic 30.0%
! GATACACCTTTTTCTCATAT+TGG - chr8_2:63476567-63476586 Msa1261160:CDS 30.0%
GTAATGCCAATATGAGAAAA+AGG + chr8_2:63476576-63476595 None:intergenic 30.0%
! GAGACCTGAAATCTTTTTAA+CGG + chr8_2:63476688-63476707 None:intergenic 30.0%
AGCAATTGACGTTAAAATTG+TGG + chr8_2:63476757-63476776 None:intergenic 30.0%
TTATCAAGCAAGTCTTTGAA+AGG - chr8_2:63476873-63476892 Msa1261160:five_prime_UTR 30.0%
GATACAAGAACTGAATCAAA+AGG + chr8_2:63476928-63476947 None:intergenic 30.0%
ATAGAACTAGAAACCAGATA+TGG + chr8_2:63476950-63476969 None:intergenic 30.0%
CAAAAATATCATTCTGATGC+TGG + chr8_2:63477004-63477023 None:intergenic 30.0%
! CAGCATCAGAATGATATTTT+TGG - chr8_2:63477002-63477021 Msa1261160:intron 30.0%
!!! CTTTTTGGGATTGACTATTA+GGG + chr8_2:63477096-63477115 None:intergenic 30.0%
!!! TCTTTTTGGGATTGACTATT+AGG + chr8_2:63477097-63477116 None:intergenic 30.0%
TTGAGTGAGTTTCCGAAATT+GGG + chr8_2:63474212-63474231 None:intergenic 35.0%
TTTGAGTGAGTTTCCGAAAT+TGG + chr8_2:63474213-63474232 None:intergenic 35.0%
ATTTCGGAAACTCACTCAAA+TGG - chr8_2:63474213-63474232 Msa1261160:three_prime_UTR 35.0%
! ATGTCTCTTCACGTTTTTGT+TGG - chr8_2:63474358-63474377 Msa1261160:CDS 35.0%
ATTGATTAAACTTGTGCGGA+TGG - chr8_2:63474756-63474775 Msa1261160:intron 35.0%
ACGAATAGTGATCTTAGACT+TGG + chr8_2:63474992-63475011 None:intergenic 35.0%
! TAATTAGGGTACTCTCTACA+GGG - chr8_2:63475138-63475157 Msa1261160:intron 35.0%
!! AAGCCACATTTTAGTACTTG+TGG - chr8_2:63475172-63475191 Msa1261160:intron 35.0%
!! AGCCACATTTTAGTACTTGT+GGG - chr8_2:63475173-63475192 Msa1261160:intron 35.0%
ATCCCACAAGTACTAAAATG+TGG + chr8_2:63475178-63475197 None:intergenic 35.0%
TAAAGCTACAATCAGAACAG+AGG + chr8_2:63475421-63475440 None:intergenic 35.0%
! CTGTTCTGATTGTAGCTTTA+TGG - chr8_2:63475421-63475440 Msa1261160:intron 35.0%
CTTTATGGGTACTAACAGAA+GGG - chr8_2:63475436-63475455 Msa1261160:intron 35.0%
TTGGAGTTCTCAGTAATGAT+CGG - chr8_2:63475565-63475584 Msa1261160:CDS 35.0%
AAAGAGGTTATTCACCTTGA+AGG + chr8_2:63475607-63475626 None:intergenic 35.0%
CACATTAGTTGAATGTTCCA+GGG + chr8_2:63475790-63475809 None:intergenic 35.0%
ACACATTAGTTGAATGTTCC+AGG + chr8_2:63475791-63475810 None:intergenic 35.0%
TTCTAAACTGCGAGTAATCT+TGG - chr8_2:63475821-63475840 Msa1261160:intron 35.0%
TTTATTAAGGGTTCGGATCA+GGG + chr8_2:63476063-63476082 None:intergenic 35.0%
TGGATCCTTTATTAAGGGTT+CGG + chr8_2:63476070-63476089 None:intergenic 35.0%
! TGATATTTTATCAGCCTGTG+AGG - chr8_2:63476295-63476314 Msa1261160:intron 35.0%
! AACTTTTGACAATCAGGTGT+GGG - chr8_2:63476406-63476425 Msa1261160:intron 35.0%
AATTCATCCCTCTATGTTGT+AGG - chr8_2:63476641-63476660 Msa1261160:intron 35.0%
! CCTGAAATCTTTTTAACGGT+TGG + chr8_2:63476684-63476703 None:intergenic 35.0%
CCAACCGTTAAAAAGATTTC+AGG - chr8_2:63476681-63476700 Msa1261160:intron 35.0%
AATCGAATGATAGAATAGGG+CGG - chr8_2:63476846-63476865 Msa1261160:five_prime_UTR 35.0%
! TCAAAGACTTGCTTGATAAG+AGG + chr8_2:63476872-63476891 None:intergenic 35.0%
AAAGGAGGAAGCCAAATAAA+AGG - chr8_2:63476891-63476910 Msa1261160:five_prime_UTR 35.0%
AAGGAGGAAGCCAAATAAAA+GGG - chr8_2:63476892-63476911 Msa1261160:five_prime_UTR 35.0%
TCAGTTCTTGTATCCATATC+TGG - chr8_2:63476934-63476953 Msa1261160:five_prime_UTR 35.0%
ATGAAGAGGATGATTATGCA+TGG - chr8_2:63477056-63477075 Msa1261160:five_prime_UTR 35.0%
!! TGATTATGCATGGCTTTGTT+AGG - chr8_2:63477066-63477085 Msa1261160:five_prime_UTR 35.0%
GGATCCACTGAGAAATAGAT+GGG + chr8_2:63474240-63474259 None:intergenic 40.0%
AAAAATTCCCTCAGCACTTC+TGG - chr8_2:63474313-63474332 Msa1261160:CDS 40.0%
TTCGTCTGCACTCACAAAAA+AGG - chr8_2:63474520-63474539 Msa1261160:CDS 40.0%
TCGTCTGCACTCACAAAAAA+GGG - chr8_2:63474521-63474540 Msa1261160:CDS 40.0%
AAAAGGGACAAGATACGAAG+CGG - chr8_2:63474537-63474556 Msa1261160:CDS 40.0%
GATCCTGAATTCAAACAAGG+TGG - chr8_2:63474566-63474585 Msa1261160:CDS 40.0%
! TGTCCACCTTGTTTGAATTC+AGG + chr8_2:63474572-63474591 None:intergenic 40.0%
AACAAGGTGGACAAAGAAAC+TGG - chr8_2:63474579-63474598 Msa1261160:CDS 40.0%
TTAAACTTGTGCGGATGGAT+AGG - chr8_2:63474761-63474780 Msa1261160:intron 40.0%
TAAACTTGTGCGGATGGATA+GGG - chr8_2:63474762-63474781 Msa1261160:intron 40.0%
! GGTTGACTTTGTCTCTACAA+TGG - chr8_2:63474783-63474802 Msa1261160:intron 40.0%
ATCCTGAATTAGTCCGTTTG+TGG - chr8_2:63474921-63474940 Msa1261160:intron 40.0%
TCCTGAATTAGTCCGTTTGT+GGG - chr8_2:63474922-63474941 Msa1261160:intron 40.0%
!! ATACCTCTCCACTTTTGCAT+AGG - chr8_2:63475101-63475120 Msa1261160:intron 40.0%
TTAGCGTTCCTATGCAAAAG+TGG + chr8_2:63475112-63475131 None:intergenic 40.0%
! CTAATTAGGGTACTCTCTAC+AGG - chr8_2:63475137-63475156 Msa1261160:intron 40.0%
CTTGGATGCATTCTTTGACT+TGG - chr8_2:63475219-63475238 Msa1261160:intron 40.0%
TGGCATCGCTAAATTGATTG+AGG - chr8_2:63475292-63475311 Msa1261160:intron 40.0%
GGCATCGCTAAATTGATTGA+GGG - chr8_2:63475293-63475312 Msa1261160:intron 40.0%
TCGCTAAATTGATTGAGGGA+TGG - chr8_2:63475297-63475316 Msa1261160:intron 40.0%
TTTGTCACATTATGGTGAGG+CGG - chr8_2:63475324-63475343 Msa1261160:intron 40.0%
! ATACAAGTTCTGGCCAATAG+TGG - chr8_2:63475390-63475409 Msa1261160:intron 40.0%
GCTTTATGGGTACTAACAGA+AGG - chr8_2:63475435-63475454 Msa1261160:intron 40.0%
! GTGCATGAACTCTAAAGACT+CGG - chr8_2:63475467-63475486 Msa1261160:CDS 40.0%
AATAACACCACCAATGAGAG+CGG + chr8_2:63475502-63475521 None:intergenic 40.0%
TTGAAGGTTGTGATTAGACG+AGG + chr8_2:63475591-63475610 None:intergenic 40.0%
TCGTCTAATCACAACCTTCA+AGG - chr8_2:63475590-63475609 Msa1261160:CDS 40.0%
TTCAGTTGTAGCAGATAGAG+TGG - chr8_2:63475712-63475731 Msa1261160:intron 40.0%
AATTTGTGATTGTGTGGGTG+TGG - chr8_2:63475945-63475964 Msa1261160:intron 40.0%
ATTTGTGATTGTGTGGGTGT+GGG - chr8_2:63475946-63475965 Msa1261160:intron 40.0%
TACATTGCCATGGAAAGGAA+GGG + chr8_2:63476036-63476055 None:intergenic 40.0%
TTACATTGCCATGGAAAGGA+AGG + chr8_2:63476037-63476056 None:intergenic 40.0%
GTTGTTACATTGCCATGGAA+AGG + chr8_2:63476041-63476060 None:intergenic 40.0%
CTTTATTAAGGGTTCGGATC+AGG + chr8_2:63476064-63476083 None:intergenic 40.0%
CTGATCCGAACCCTTAATAA+AGG - chr8_2:63476062-63476081 Msa1261160:intron 40.0%
TAACTCTAGTCTCTAGCCAA+TGG + chr8_2:63476122-63476141 None:intergenic 40.0%
! ATTTTATCAGCCTGTGAGGA+AGG - chr8_2:63476299-63476318 Msa1261160:intron 40.0%
! TTTTATCAGCCTGTGAGGAA+GGG - chr8_2:63476300-63476319 Msa1261160:intron 40.0%
! AATGGTGGTGTGACAAAAAC+AGG - chr8_2:63476327-63476346 Msa1261160:intron 40.0%
! GAACTTTTGACAATCAGGTG+TGG - chr8_2:63476405-63476424 Msa1261160:intron 40.0%
CCAACAACCTACAACATAGA+GGG + chr8_2:63476651-63476670 None:intergenic 40.0%
TCCAACAACCTACAACATAG+AGG + chr8_2:63476652-63476671 None:intergenic 40.0%
TTGTAGGTTGTTGGAAATCC+AGG - chr8_2:63476657-63476676 Msa1261160:intron 40.0%
!! ATCTTTTTAACGGTTGGTCC+TGG + chr8_2:63476678-63476697 None:intergenic 40.0%
! TCAAGCAAGTCTTTGAAAGG+AGG - chr8_2:63476876-63476895 Msa1261160:five_prime_UTR 40.0%
GGTTGCATTCCAAACCGAAT+AGG + chr8_2:63474023-63474042 None:intergenic 45.0%
AAGAACAACCAACTACCACG+AGG + chr8_2:63474130-63474149 None:intergenic 45.0%
! TGGACCCATCTATTTCTCAG+TGG - chr8_2:63474233-63474252 Msa1261160:three_prime_UTR 45.0%
CGGATCCACTGAGAAATAGA+TGG + chr8_2:63474241-63474260 None:intergenic 45.0%
!! GTGAGCTCTGAATGCGATTA+AGG + chr8_2:63474290-63474309 None:intergenic 45.0%
TAATCGCATTCAGAGCTCAC+AGG - chr8_2:63474290-63474309 Msa1261160:CDS 45.0%
TCAATACAATCGTCCACCAG+CGG + chr8_2:63474902-63474921 None:intergenic 45.0%
GTTCCTATGCAAAAGTGGAG+AGG + chr8_2:63475107-63475126 None:intergenic 45.0%
AACAGAGGCAATGCCACTAT+TGG + chr8_2:63475406-63475425 None:intergenic 45.0%
ATGGGTACTAACAGAAGGGA+AGG - chr8_2:63475440-63475459 Msa1261160:intron 45.0%
GCTCTAATTACCGCTCTCAT+TGG - chr8_2:63475489-63475508 Msa1261160:CDS 45.0%
CTAATTACCGCTCTCATTGG+TGG - chr8_2:63475492-63475511 Msa1261160:CDS 45.0%
!! GCTCTCATTGGTGGTGTTAT+TGG - chr8_2:63475501-63475520 Msa1261160:CDS 45.0%
CTCCATTGCAGCAGCAATAA+TGG + chr8_2:63475527-63475546 None:intergenic 45.0%
ATTGAAAGGGCTGGGTCATT+AGG - chr8_2:63475971-63475990 Msa1261160:intron 45.0%
AACATTCCCCTTCCTTTCCA+TGG - chr8_2:63476026-63476045 Msa1261160:intron 45.0%
ACATTGCCATGGAAAGGAAG+GGG + chr8_2:63476035-63476054 None:intergenic 45.0%
TCAGGGTTGTTACATTGCCA+TGG + chr8_2:63476046-63476065 None:intergenic 45.0%
ACATTCCCCTTCCTTTCCAT+TGG - chr8_2:63476027-63476046 Msa1261160:intron 45.0%
TCTAGCCAATGGAAAGGAAG+GGG + chr8_2:63476111-63476130 None:intergenic 45.0%
CTCTAGCCAATGGAAAGGAA+GGG + chr8_2:63476112-63476131 None:intergenic 45.0%
TCTCTAGCCAATGGAAAGGA+AGG + chr8_2:63476113-63476132 None:intergenic 45.0%
CTAGTCTCTAGCCAATGGAA+AGG + chr8_2:63476117-63476136 None:intergenic 45.0%
! TGTGGCAGCATGGCATTTTA+AGG + chr8_2:63476156-63476175 None:intergenic 45.0%
GGCGCAACATTGCAATTTAG+TGG - chr8_2:63476513-63476532 Msa1261160:CDS 45.0%
ATTCTGCTCTATTCGCCAGA+AGG - chr8_2:63476536-63476555 Msa1261160:CDS 45.0%
CCCTCTATGTTGTAGGTTGT+TGG - chr8_2:63476648-63476667 Msa1261160:intron 45.0%
GAGGAAGCCAAATAAAAGGG+CGG - chr8_2:63476895-63476914 Msa1261160:five_prime_UTR 45.0%
AGCCAAATAAAAGGGCGGAA+AGG - chr8_2:63476900-63476919 Msa1261160:five_prime_UTR 45.0%
GCCAAATAAAAGGGCGGAAA+GGG - chr8_2:63476901-63476920 Msa1261160:five_prime_UTR 45.0%
! ACCCTTTCCGCCCTTTTATT+TGG + chr8_2:63476905-63476924 None:intergenic 45.0%
CGTGTTCCTCGTTGCCTATT+CGG - chr8_2:63474006-63474025 Msa1261160:three_prime_UTR 50.0%
TCCTCGTTGCCTATTCGGTT+TGG - chr8_2:63474011-63474030 Msa1261160:three_prime_UTR 50.0%
TCCAAACCGAATAGGCAACG+AGG + chr8_2:63474015-63474034 None:intergenic 50.0%
AAAAGTTCCACTCCACCTCG+TGG - chr8_2:63474112-63474131 Msa1261160:three_prime_UTR 50.0%
AACAACCAACTACCACGAGG+TGG + chr8_2:63474127-63474146 None:intergenic 50.0%
TTCTCAGTGGATCCGACCAT+TGG - chr8_2:63474246-63474265 Msa1261160:three_prime_UTR 50.0%
CAGTGACAGCAACCAATGGT+CGG + chr8_2:63474261-63474280 None:intergenic 50.0%
CCAACAGTGACAGCAACCAA+TGG + chr8_2:63474265-63474284 None:intergenic 50.0%
! CCATTGGTTGCTGTCACTGT+TGG - chr8_2:63474262-63474281 Msa1261160:exon 50.0%
!! AAGTGCACCAGAAGTGCTGA+GGG + chr8_2:63474323-63474342 None:intergenic 50.0%
AATACAATCGTCCACCAGCG+GGG + chr8_2:63474900-63474919 None:intergenic 50.0%
CAATACAATCGTCCACCAGC+GGG + chr8_2:63474901-63474920 None:intergenic 50.0%
!! GCCCACAAACGGACTAATTC+AGG + chr8_2:63474926-63474945 None:intergenic 50.0%
GAATTAGTCCGTTTGTGGGC+CGG - chr8_2:63474926-63474945 Msa1261160:intron 50.0%
TTCAGTATCCGGCCCACAAA+CGG + chr8_2:63474937-63474956 None:intergenic 50.0%
TGAGGCGGTTGACCTGAATT+TGG - chr8_2:63475339-63475358 Msa1261160:intron 50.0%
GGCCATTATTGCTGCTGCAA+TGG - chr8_2:63475522-63475541 Msa1261160:CDS 50.0%
GACACATGGTCTTCTGAGCT+TGG - chr8_2:63475546-63475565 Msa1261160:CDS 50.0%
TGTGGGTGTGGGACATTGAA+AGG - chr8_2:63475957-63475976 Msa1261160:intron 50.0%
GTGGGTGTGGGACATTGAAA+GGG - chr8_2:63475958-63475977 Msa1261160:intron 50.0%
TGTGGGACATTGAAAGGGCT+GGG - chr8_2:63475963-63475982 Msa1261160:intron 50.0%
AAATGCCATGCTGCCACAAG+TGG - chr8_2:63476158-63476177 Msa1261160:intron 50.0%
ATAAGCCACTTGTGGCAGCA+TGG + chr8_2:63476166-63476185 None:intergenic 50.0%
CCATTTGTACCCTTCCTCAC+AGG + chr8_2:63476312-63476331 None:intergenic 50.0%
! CCTGTGAGGAAGGGTACAAA+TGG - chr8_2:63476309-63476328 Msa1261160:intron 50.0%
! GTGAGGAAGGGTACAAATGG+TGG - chr8_2:63476312-63476331 Msa1261160:intron 50.0%
ACTTCCACATAGTCCTGCCA+CGG + chr8_2:63476475-63476494 None:intergenic 50.0%
!! CAAGTGCACCAGAAGTGCTG+AGG + chr8_2:63474324-63474343 None:intergenic 55.0%
AGCACTTCTGGTGCACTTGC+TGG - chr8_2:63474325-63474344 Msa1261160:CDS 55.0%
ATACAATCGTCCACCAGCGG+GGG + chr8_2:63474899-63474918 None:intergenic 55.0%
GCTGCTGCAATGGAGACACA+TGG - chr8_2:63475532-63475551 Msa1261160:CDS 55.0%
GTGTGGGACATTGAAAGGGC+TGG - chr8_2:63475962-63475981 Msa1261160:intron 55.0%
!! GCAGCTGGTGGAAGTGTCAT+TGG - chr8_2:63476363-63476382 Msa1261160:intron 55.0%
TAGTCCTGCCACGGTAGTGA+GGG + chr8_2:63476466-63476485 None:intergenic 55.0%
ATAGTCCTGCCACGGTAGTG+AGG + chr8_2:63476467-63476486 None:intergenic 55.0%
ACTACCGTGGCAGGACTATG+TGG - chr8_2:63476468-63476487 Msa1261160:intron 55.0%
CCAACTACCACGAGGTGGAG+TGG + chr8_2:63474122-63474141 None:intergenic 60.0%
CCACTCCACCTCGTGGTAGT+TGG - chr8_2:63474119-63474138 Msa1261160:three_prime_UTR 60.0%
TACAATCGTCCACCAGCGGG+GGG + chr8_2:63474898-63474917 None:intergenic 60.0%
GGACTTCTAGCAGCTGCAGC+TGG - chr8_2:63476348-63476367 Msa1261160:intron 60.0%
CTTCTAGCAGCTGCAGCTGG+TGG - chr8_2:63476351-63476370 Msa1261160:intron 60.0%
GGTCATACCCCTCACTACCG+TGG - chr8_2:63476455-63476474 Msa1261160:intron 60.0%
ATACCCCTCACTACCGTGGC+AGG - chr8_2:63476459-63476478 Msa1261160:intron 60.0%
AGTCCTGCCACGGTAGTGAG+GGG + chr8_2:63476465-63476484 None:intergenic 60.0%
CCAGCGGGGGGTCCAATTGT+TGG + chr8_2:63474886-63474905 None:intergenic 65.0%
CCAACAATTGGACCCCCCGC+TGG - chr8_2:63474883-63474902 Msa1261160:intron 65.0%
ACAATTGGACCCCCCGCTGG+TGG - chr8_2:63474886-63474905 Msa1261160:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 63473947 63477126 63473947 ID=Msa1261160;Name=Msa1261160
chr8_2 mRNA 63473947 63477126 63473947 ID=Msa1261160-mRNA-1;Parent=Msa1261160;Name=Msa1261160-mRNA-1;_AED=0.08;_eAED=0.08;_QI=261|1|1|1|0.8|0.66|6|332|290
chr8_2 exon 63473947 63474410 63473947 ID=Msa1261160-mRNA-1:exon:20312;Parent=Msa1261160-mRNA-1
chr8_2 exon 63474516 63474764 63474516 ID=Msa1261160-mRNA-1:exon:20311;Parent=Msa1261160-mRNA-1
chr8_2 exon 63475462 63475684 63475462 ID=Msa1261160-mRNA-1:exon:20310;Parent=Msa1261160-mRNA-1
chr8_2 exon 63476473 63476586 63476473 ID=Msa1261160-mRNA-1:exon:20309;Parent=Msa1261160-mRNA-1
chr8_2 exon 63476687 63476997 63476687 ID=Msa1261160-mRNA-1:exon:20308;Parent=Msa1261160-mRNA-1
chr8_2 exon 63477022 63477126 63477022 ID=Msa1261160-mRNA-1:exon:20307;Parent=Msa1261160-mRNA-1
chr8_2 five_prime_UTR 63477022 63477126 63477022 ID=Msa1261160-mRNA-1:five_prime_utr;Parent=Msa1261160-mRNA-1
chr8_2 five_prime_UTR 63476842 63476997 63476842 ID=Msa1261160-mRNA-1:five_prime_utr;Parent=Msa1261160-mRNA-1
chr8_2 CDS 63476687 63476841 63476687 ID=Msa1261160-mRNA-1:cds;Parent=Msa1261160-mRNA-1
chr8_2 CDS 63476473 63476586 63476473 ID=Msa1261160-mRNA-1:cds;Parent=Msa1261160-mRNA-1
chr8_2 CDS 63475462 63475684 63475462 ID=Msa1261160-mRNA-1:cds;Parent=Msa1261160-mRNA-1
chr8_2 CDS 63474516 63474764 63474516 ID=Msa1261160-mRNA-1:cds;Parent=Msa1261160-mRNA-1
chr8_2 CDS 63474279 63474410 63474279 ID=Msa1261160-mRNA-1:cds;Parent=Msa1261160-mRNA-1
chr8_2 three_prime_UTR 63473947 63474278 63473947 ID=Msa1261160-mRNA-1:three_prime_utr;Parent=Msa1261160-mRNA-1
Gene Sequence

>Msa1261160

ATGGACCCATCTATTTCTCAGTGGATCCGACCATTGGTTGCTGTCACTGTTGGTTCCTTAATCGCATTCAGAGCTCACAGGAAAAATTCCCTCAGCACTTCTGGTGCACTTGCTGGTTTCTTCGTTATGTCTCTTCACGTTTTTGTTGGATTTAGGTTTGCGGCTATGATATTTGCTTTCTTTTTCACTTCGTCTGCACTCACAAAAAAGGGACAAGATACGAAGCGGTTGATTGATCCTGAATTCAAACAAGGTGGACAAAGAAACTGGATACAAGTTCTGGCCAATAGTGGCATTGCCTCTGTTCTGATTGTAGCTTTATGGGTACTAACAGAAGGGAAGGATCAGTGCATGAACTCTAAAGACTCGGCTCTAATTACCGCTCTCATTGGTGGTGTTATTGGCCATTATTGCTGCTGCAATGGAGACACATGGTCTTCTGAGCTTGGAGTTCTCAGTAATGATCGGCCTCGTCTAATCACAACCTTCAAGCCTGTGAGGAAGGGTACAAATGGTGGTGTGACAAAAACAGGACTTCTAGCAGCTGCAGCTGGTGGAAGTGTCATTGGAGTGTCGTATGTTCTTCTAGAACTTTTGACAATCAGGTGTGGGTCTGATAGAATTCTCAAGCAACTATTGGTCATACCCCTCACTACCGTGGCAGGACTATGTGGAAGTATCATTGACTCTCTATTGGGCGCAACATTGCAATTTAGTGGATTCTGCTCTATTCGCCAGAAGGTTGTTGGAAATCCAGGACCAACCGTTAAAAAGATTTCAGGTCTCAGTATTCTTGACAACAATGCAGTGAACTTTGTGTCAATACTATTGACCACAATTTTAACGTCAATTGCTTGTTTGTACATATTTTAA
Protein sequence

>Msa1261160

MDPSISQWIRPLVAVTVGSLIAFRAHRKNSLSTSGALAGFFVMSLHVFVGFRFAAMIFAFFFTSSALTKKGQDTKRLIDPEFKQGGQRNWIQVLANSGIASVLIVALWVLTEGKDQCMNSKDSALITALIGGVIGHYCCCNGDTWSSELGVLSNDRPRLITTFKPVRKGTNGGVTKTGLLAAAAGGSVIGVSYVLLELLTIRCGSDRILKQLLVIPLTTVAGLCGSIIDSLLGATLQFSGFCSIRQKVVGNPGPTVKKISGLSILDNNAVNFVSILLTTILTSIACLYIF*