Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0300470 | GAU27075.1 | 91.966 | 585 | 41 | 4 | 1 | 584 | 1 | 580 | 0.0 | 1087 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0300470 | sp|O82043|ILV5_PEA | 85.983 | 585 | 77 | 3 | 1 | 584 | 1 | 581 | 0.0 | 1026 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0300470 | A0A2Z6MTP4 | 91.966 | 585 | 41 | 4 | 1 | 584 | 1 | 580 | 0.0 | 1087 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0007810 | Msa0300470 | -0.820802 | 5.658067e-53 | -8.615850e-47 |
| Msa0025560 | Msa0300470 | 0.808127 | 3.607342e-50 | -8.615850e-47 |
| Msa0026250 | Msa0300470 | 0.807425 | 5.088011e-50 | -8.615850e-47 |
| Msa0028690 | Msa0300470 | 0.815642 | 8.326574e-52 | -8.615850e-47 |
| Msa0032890 | Msa0300470 | -0.805014 | 1.637919e-49 | -8.615850e-47 |
| Msa0032930 | Msa0300470 | -0.809199 | 2.128835e-50 | -8.615850e-47 |
| Msa0033220 | Msa0300470 | 0.809878 | 1.522280e-50 | -8.615850e-47 |
| Msa0034700 | Msa0300470 | -0.847021 | 1.504567e-59 | -8.615850e-47 |
| Msa0038150 | Msa0300470 | -0.850281 | 1.879578e-60 | -8.615850e-47 |
| Msa0038530 | Msa0300470 | 0.842524 | 2.453811e-58 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0300470 | MtrunA17_Chr4g0041071 | 86.644 | 584 | 73 | 3 | 1 | 583 | 1 | 580 | 0.0 | 1036 |
| Msa0300470 | MtrunA17_Chr2g0325551 | 96.078 | 255 | 10 | 0 | 330 | 584 | 1 | 255 | 0.0 | 516 |
| Msa0300470 | MtrunA17_Chr2g0325541 | 96.680 | 241 | 8 | 0 | 60 | 300 | 1 | 241 | 1.16e-168 | 478 |
| Msa0300470 | MtrunA17_Chr7g0226301 | 81.481 | 54 | 9 | 1 | 60 | 113 | 1 | 53 | 1.23e-23 | 94.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0300470 | AT3G58610.3 | 88.972 | 535 | 58 | 1 | 50 | 584 | 58 | 591 | 0.0 | 995 |
| Msa0300470 | AT3G58610.1 | 88.972 | 535 | 58 | 1 | 50 | 584 | 58 | 591 | 0.0 | 995 |
| Msa0300470 | AT3G58610.2 | 88.972 | 535 | 58 | 1 | 50 | 584 | 58 | 591 | 0.0 | 995 |
Find 202 sgRNAs with CRISPR-Local
Find 546 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGTCTGATATTGTAGTTA+AGG | 0.449986 | 2_3:+55928794 | Msa0300470:CDS |
| AAATACAACCTTAGTAGAGA+TGG | 0.570137 | 2_3:-55930815 | None:intergenic |
| AAATTGACCAGACCCGCATG+TGG | 0.643474 | 2_3:+55931242 | Msa0300470:CDS |
| AAATTGCAGTTTATAGTAAG+AGG | 0.437476 | 2_3:+55928431 | Msa0300470:intron |
| AACACCGTTGAGAGTATAAC+AGG | 0.395910 | 2_3:+55930778 | Msa0300470:CDS |
| AACCATGAAGGAAACACCAC+GGG | 0.609978 | 2_3:-55931580 | None:intergenic |
| AACTCCTGTTATACTCTCAA+CGG | 0.581395 | 2_3:-55930782 | None:intergenic |
| AAGAAGAAGATTGGAGTGTG+AGG | 0.530608 | 2_3:-55928149 | None:intergenic |
| AAGAGCTACTAATGTTGCTT+TGG | 0.368714 | 2_3:+55930507 | Msa0300470:CDS |
| AAGATACCATACGCGCGGCG+AGG | 0.629294 | 2_3:-55928230 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATTGTGATAAGGAAAGAAAG+AGG | + | chr2_3:55926482-55926501 | Msa0300470:intron | 30.0% | |
| AAGGAAAGAAAGAATATAGG+TGG | + | chr2_3:55926550-55926569 | Msa0300470:intron | 30.0% | |
| AGGAAAGAAAGAATATAGGT+GGG | + | chr2_3:55926551-55926570 | Msa0300470:intron | 30.0% | |
| TAAAAAGCCCGGTTTAAATA+TGG | + | chr2_3:55926680-55926699 | Msa0300470:intron | 30.0% | |
| AAAAAGCCCGGTTTAAATAT+GGG | + | chr2_3:55926681-55926700 | Msa0300470:intron | 30.0% | |
| ATTTCAGCCCATATTTAAAC+CGG | - | chr2_3:55926691-55926710 | None:intergenic | 30.0% | |
| AAAATACATGTAGTACATGC+GGG | - | chr2_3:55926768-55926787 | None:intergenic | 30.0% | |
| GAGCTAATTTACCATACAAA+AGG | - | chr2_3:55926877-55926896 | None:intergenic | 30.0% | |
| AAGCATGCCTAAAATTTGAT+TGG | - | chr2_3:55926963-55926982 | None:intergenic | 30.0% | |
| TGCTGTCAACATAGTTTAAT+GGG | - | chr2_3:55927027-55927046 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_3 | gene | 55926308 | 55932179 | 55926308 | ID=Msa0300470;Name=Msa0300470 |
| chr2_3 | mRNA | 55926308 | 55932179 | 55926308 | ID=Msa0300470-mRNA-1;Parent=Msa0300470;Name=Msa0300470-mRNA-1;_AED=0.08;_eAED=0.08;_QI=171|1|1|1|0.9|0.81|11|325|584 |
| chr2_3 | exon | 55926308 | 55926380 | 55926308 | ID=Msa0300470-mRNA-1:exon:17859;Parent=Msa0300470-mRNA-1 |
| chr2_3 | exon | 55927968 | 55928341 | 55927968 | ID=Msa0300470-mRNA-1:exon:17860;Parent=Msa0300470-mRNA-1 |
| chr2_3 | exon | 55928440 | 55928532 | 55928440 | ID=Msa0300470-mRNA-1:exon:17861;Parent=Msa0300470-mRNA-1 |
| chr2_3 | exon | 55928750 | 55928815 | 55928750 | ID=Msa0300470-mRNA-1:exon:17862;Parent=Msa0300470-mRNA-1 |
| chr2_3 | exon | 55929120 | 55929260 | 55929120 | ID=Msa0300470-mRNA-1:exon:17863;Parent=Msa0300470-mRNA-1 |
| chr2_3 | exon | 55930150 | 55930377 | 55930150 | ID=Msa0300470-mRNA-1:exon:17864;Parent=Msa0300470-mRNA-1 |
| chr2_3 | exon | 55930496 | 55930613 | 55930496 | ID=Msa0300470-mRNA-1:exon:17865;Parent=Msa0300470-mRNA-1 |
| chr2_3 | exon | 55930689 | 55930828 | 55930689 | ID=Msa0300470-mRNA-1:exon:17866;Parent=Msa0300470-mRNA-1 |
| chr2_3 | exon | 55930961 | 55931128 | 55930961 | ID=Msa0300470-mRNA-1:exon:17867;Parent=Msa0300470-mRNA-1 |
| chr2_3 | exon | 55931208 | 55931363 | 55931208 | ID=Msa0300470-mRNA-1:exon:17868;Parent=Msa0300470-mRNA-1 |
| chr2_3 | exon | 55931486 | 55932179 | 55931486 | ID=Msa0300470-mRNA-1:exon:17869;Parent=Msa0300470-mRNA-1 |
| chr2_3 | five_prime_UTR | 55926308 | 55926380 | 55926308 | ID=Msa0300470-mRNA-1:five_prime_utr;Parent=Msa0300470-mRNA-1 |
| chr2_3 | five_prime_UTR | 55927968 | 55928065 | 55927968 | ID=Msa0300470-mRNA-1:five_prime_utr;Parent=Msa0300470-mRNA-1 |
| chr2_3 | CDS | 55928066 | 55928341 | 55928066 | ID=Msa0300470-mRNA-1:cds;Parent=Msa0300470-mRNA-1 |
| chr2_3 | CDS | 55928440 | 55928532 | 55928440 | ID=Msa0300470-mRNA-1:cds;Parent=Msa0300470-mRNA-1 |
| chr2_3 | CDS | 55928750 | 55928815 | 55928750 | ID=Msa0300470-mRNA-1:cds;Parent=Msa0300470-mRNA-1 |
| chr2_3 | CDS | 55929120 | 55929260 | 55929120 | ID=Msa0300470-mRNA-1:cds;Parent=Msa0300470-mRNA-1 |
| chr2_3 | CDS | 55930150 | 55930377 | 55930150 | ID=Msa0300470-mRNA-1:cds;Parent=Msa0300470-mRNA-1 |
| chr2_3 | CDS | 55930496 | 55930613 | 55930496 | ID=Msa0300470-mRNA-1:cds;Parent=Msa0300470-mRNA-1 |
| chr2_3 | CDS | 55930689 | 55930828 | 55930689 | ID=Msa0300470-mRNA-1:cds;Parent=Msa0300470-mRNA-1 |
| chr2_3 | CDS | 55930961 | 55931128 | 55930961 | ID=Msa0300470-mRNA-1:cds;Parent=Msa0300470-mRNA-1 |
| chr2_3 | CDS | 55931208 | 55931363 | 55931208 | ID=Msa0300470-mRNA-1:cds;Parent=Msa0300470-mRNA-1 |
| chr2_3 | CDS | 55931486 | 55931854 | 55931486 | ID=Msa0300470-mRNA-1:cds;Parent=Msa0300470-mRNA-1 |
| chr2_3 | three_prime_UTR | 55931855 | 55932179 | 55931855 | ID=Msa0300470-mRNA-1:three_prime_utr;Parent=Msa0300470-mRNA-1 |
| Gene Sequence |
>Msa0300470 ATGGCGGCTAAGACGTCGTTTTCTCCATCTATCTCCTCCACTTCTCAAATTCTTCAACCCAAAACCTTCATCACCTCCAACAACCTCACACTCCAATCTTCTTCTTCTTCCAGACTCTTCAAAACCTTAACTCTTCACCGTCGCACGGGCATCGTCGCCTCATCCCTCGCCGCGCGTATGGTATCTTCGCCGCCGGCTACTAAACTCCCCGCCTCACTCGATTTTGATACGTCGCTTTTTAAAAAGGAAAAAGTCAACCTCGCCGGACACGAAGAGTTTATAGTAAGAGGTGGCAGGGATTTGTTCCATTTGTTGCCAGATGCTTTCAAAGGAATTAAGCAGATTGGTGTTATCGGTTGGGGATCACAGGGACCTGCTCAAGCTCAAAATCTAAGGGACTCTCTTGCTGAAGCAAAGTCTGATATTGTAGTTAAGGTTGGATTGAGGAAAGGTTCTAGTTCCTTTGCTGAAGCTCGAGATGCTGGGTTTTCTGAAGAAAGTGGGACTCTAGGTGACATGTGGGAAACTATCTCGGGCAGTGATCTCGTGCTGCTTTTGATTTCTGATTCTGCTCAGGCAGATAACTATGAAAAAATATTATCCCACATGAAGCCAAACAGCATACTAGGGCTGTCCCATGGTTTTCTTCTTGGGCATTTACAGTCTTTGGGGCATGATTTTCCAAAGAACATTAGTGTAATTGCTGTGTGCCCAAAAGGTATGGGTCCATCTGTTAGGAGGCTATATGTCCAAGGCAAAGAGATAAATGGGGCAGGAATTAATTCAAGTTTTGCAGTCCACCGAGATGTGGATGGAAGAGCTACTAATGTTGCTTTGGGGTGGTCTGTTGCTCTGGGTTCTCCATTCACTTTCGCCACTACCTTGGAGCAGGAATACAAGAGCGATATCTTTGGGGAGAGAGGGATATTACTTGGTGCTGTTCATGGAATTGTGGAATCCTTATTTAGGAGATATACTGAAAGTGGAATGGCCGAGGATTTAGCTTATAAAAACACCGTTGAGAGTATAACAGGAGTTATATCTAGAACCATCTCTACTAAGGGCATGCTAGCTGTATACAATGCTTTGTCTGAAGATGGGAAAAAGGAATTTGAGAAAGCTTACAGTGCTTCTTTTTATCCCTGCATGGAAATTTTGTATGAGTGCTATGAGGATGTAGCCAGTGGCAGTGAGATTCGTAGTGTTGTTCTGGCTGGGCGTCGTTTTTACGAGAAAGAAGGTCTGCCTGCATTTCCCATGGGTAAAATTGACCAGACCCGCATGTGGAAGGTTGGTGAGCGTGTCCGGTCTACAAGACCAGCAGGTGATCAAGGCCCATTATACCCTTTTACTGCTGGTGTCTTTGTGGCACTGATGATGGCCCAGATTGAGATCCTAAGAAGGAAAGGACATTCCTACTCTGAAATCATTAACGAGAGTCTTATTGAGGCTGTTGATTCTTTGAATCCGTTCATGCATGCCCGTGGTGTTTCCTTCATGGTTGACAATTGTTCAACCACTGCTAGGTTGGGTTCGAGGAAATGGGCTCCACGGTTTGATTACAACCTAACCCAGCAGACCTTTGTGGCAGTGGACAATGGTGCCCCTATTAACCGGGACCTAATCAGCAACTTCATATCAGATCCAGTGCATGGCGCCATTGAAGTTTGTGCTGAACTGAGACCTACGGTAGACATTTCTGTTCCTGCTGATGCAGACTTTGTTCGCCCAGAGTTGCGCCAATCCAACAATTAG |
| Protein sequence |
>Msa0300470 MAAKTSFSPSISSTSQILQPKTFITSNNLTLQSSSSSRLFKTLTLHRRTGIVASSLAARMVSSPPATKLPASLDFDTSLFKKEKVNLAGHEEFIVRGGRDLFHLLPDAFKGIKQIGVIGWGSQGPAQAQNLRDSLAEAKSDIVVKVGLRKGSSSFAEARDAGFSEESGTLGDMWETISGSDLVLLLISDSAQADNYEKILSHMKPNSILGLSHGFLLGHLQSLGHDFPKNISVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHRDVDGRATNVALGWSVALGSPFTFATTLEQEYKSDIFGERGILLGAVHGIVESLFRRYTESGMAEDLAYKNTVESITGVISRTISTKGMLAVYNALSEDGKKEFEKAYSASFYPCMEILYECYEDVASGSEIRSVVLAGRRFYEKEGLPAFPMGKIDQTRMWKVGERVRSTRPAGDQGPLYPFTAGVFVALMMAQIEILRRKGHSYSEIINESLIEAVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYNLTQQTFVAVDNGAPINRDLISNFISDPVHGAIEVCAELRPTVDISVPADADFVRPELRQSNN* |
