AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1327510

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1327510 MtrunA17_Chr8g0366031 100.000 255 0 0 1 255 737 991 0.0 538
Msa1327510 MtrunA17_Chr7g0220451 81.569 255 47 0 1 255 826 1080 5.30e-150 446
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1327510 AT5G15700.1 74.510 255 65 0 1 255 757 1011 1.43e-138 414
Msa1327510 AT1G68990.2 74.902 255 64 0 1 255 729 983 2.23e-137 410
Msa1327510 AT1G68990.1 74.902 255 64 0 1 255 722 976 2.26e-137 410
Msa1327510 AT2G24120.1 72.941 255 69 0 1 255 739 993 2.69e-135 405
Msa1327510 AT5G15700.2 74.286 245 63 0 1 245 757 1001 7.51e-132 398
Msa1327510 AT2G24120.2 68.707 147 46 0 1 147 739 885 1.27e-62 211

Find 95 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAGAGAGTGAACAAAATT+TGG 0.151766 8_4:-44884181 None:intergenic
CAGGGGTGCATGATTCTTAT+TGG 0.190275 8_4:+44884354 Msa1327510:intron
TGAGGCTGCAAGAAGTATTA+TGG 0.213750 8_4:+44883522 Msa1327510:CDS
AGTAGCGCTAGAACAAATTT+TGG 0.226034 8_4:-44882871 None:intergenic
TAATATTATGATTAGGATAA+AGG 0.235217 8_4:-44882766 None:intergenic
GTATAAGTTATTTCAAATAA+TGG 0.257894 8_4:-44885648 None:intergenic
CTCTTGTGAAGCAACCAAAT+TGG 0.267918 8_4:+44885504 Msa1327510:three_prime_UTR
CAAGAGAGTGAACAAAATTT+GGG 0.306701 8_4:-44884180 None:intergenic
ACTCTACGAAACTCCGATAT+TGG 0.319241 8_4:+44884433 Msa1327510:CDS
GGAACTTAGTACTCCTTGTT+TGG 0.329894 8_4:+44885272 Msa1327510:three_prime_UTR
CGTTACAGGTCATGGTAAAA+AGG 0.336398 8_4:+44884140 Msa1327510:intron
CTGTCTGCTTTACCAACTTT+CGG 0.346118 8_4:-44883269 None:intergenic
AGGATTGAAGTGATCATATA+AGG 0.350570 8_4:+44885456 Msa1327510:three_prime_UTR
GTTCCTGCAGGAGACTAATA+TGG 0.355954 8_4:-44885254 None:intergenic
TGTAACATAGACTAATAAAA+AGG 0.357886 8_4:+44885436 Msa1327510:three_prime_UTR
AATATTATGATTAGGATAAA+GGG 0.365525 8_4:-44882765 None:intergenic
TGAAAGTTAATATTATGATT+AGG 0.366731 8_4:-44882773 None:intergenic
AGTCTCCTCGTTCCGGTAAA+GGG 0.367091 8_4:-44885021 None:intergenic
AATATACTATTCATAGATAA+AGG 0.371374 8_4:-44882798 None:intergenic
AGTGTTCAAAGCAAATATTT+TGG 0.372708 8_4:+44885228 Msa1327510:three_prime_UTR
TTGGTTTGTGGAGAGGGAAA+TGG 0.374510 8_4:+44885567 Msa1327510:three_prime_UTR
GAGGCTGCAAGAAGTATTAT+GGG 0.379156 8_4:+44883523 Msa1327510:CDS
AGCCTCAAACATCTCTTCTA+AGG 0.392432 8_4:-44883506 None:intergenic
CTTGTTTGGAACTAGAAATC+TGG 0.400729 8_4:+44885286 Msa1327510:three_prime_UTR
TCTTGTGAAGCAACCAAATT+GGG 0.409539 8_4:+44885505 Msa1327510:three_prime_UTR
AACGCATGTGGATCAGTTGC+AGG 0.411067 8_4:-44882966 None:intergenic
GTGCGATGGGTCACTCCCCT+TGG 0.416735 8_4:+44883708 Msa1327510:CDS
TGCGATGGGTCACTCCCCTT+GGG 0.416968 8_4:+44883709 Msa1327510:CDS
ACAGTGATGACGTCAGTCTA+TGG 0.429518 8_4:+44883288 Msa1327510:CDS
AGATCCAAGTCTCCTCGTTC+CGG 0.432465 8_4:-44885028 None:intergenic
TGTAAGATATATACTTGCAT+GGG 0.433198 8_4:+44885168 Msa1327510:three_prime_UTR
CAAGCAGGGATGAACTTTGC+AGG 0.433433 8_4:+44884241 Msa1327510:CDS
AAGAAAGAGTGCCCCACATA+TGG 0.436033 8_4:+44885327 Msa1327510:three_prime_UTR
TCTCGGTAAGGTTGTACAAC+TGG 0.437051 8_4:-44883735 None:intergenic
AAGTCTCCTCGTTCCGGTAA+AGG 0.443118 8_4:-44885022 None:intergenic
CCAGGTGTCTTCCTACTTCT+CGG 0.446736 8_4:-44883752 None:intergenic
TAAGGAGCGTTGTGCGGTTG+AGG 0.450901 8_4:+44883353 Msa1327510:CDS
TCGCACTGCTTGGTTTGTGC+AGG 0.466104 8_4:-44883691 None:intergenic
ATCCACATGCGTTGCATGCT+AGG 0.469299 8_4:+44882976 Msa1327510:CDS
TCAACTTGTAATGCCATATG+TGG 0.470354 8_4:-44885340 None:intergenic
AATAAAGGGGTTGTTGCGCC+TGG 0.484015 8_4:+44882704 None:intergenic
TTTACCGGAACGAGGAGACT+TGG 0.490240 8_4:+44885024 Msa1327510:CDS
TCTATGGAGTGACTTACATC+GGG 0.496903 8_4:+44883304 Msa1327510:CDS
TTGTAAGATATATACTTGCA+TGG 0.500352 8_4:+44885167 Msa1327510:three_prime_UTR
GCTGTAGTGTCAATACTTGA+AGG 0.503590 8_4:-44883922 None:intergenic
AGGAAATGAACAGGATACTC+AGG 0.504918 8_4:+44884396 Msa1327510:CDS
CACATTGGAGTTAACTCCAC+TGG 0.506591 8_4:-44885137 None:intergenic
TGTCTTCCTACTTCTCGGTA+AGG 0.506968 8_4:-44883747 None:intergenic
GCACAAACCAAGCAGTGCGA+TGG 0.515196 8_4:+44883694 Msa1327510:CDS
TCTTACAAATTTCATCACAT+TGG 0.522540 8_4:-44885152 None:intergenic
TGCCTTAGAAGAGATGTTTG+AGG 0.523072 8_4:+44883504 Msa1327510:CDS
CCAGATCAAAAGGAGGCTTA+AGG 0.526268 8_4:+44883335 Msa1327510:CDS
GCGACGTTGAGGAAATGAAC+AGG 0.527386 8_4:+44884387 Msa1327510:CDS
GGGCCCGAGACCAGATCAAA+AGG 0.528984 8_4:+44883325 Msa1327510:CDS
GCAGTTGCTTGTAAACAAGC+AGG 0.531102 8_4:+44884226 Msa1327510:CDS
TTGCCATATTAGTCTCCTGC+AGG 0.537031 8_4:+44885251 Msa1327510:three_prime_UTR
TGCTAGGCGTTTGATCAACC+AGG 0.539952 8_4:+44882992 Msa1327510:CDS
GAGTGACCCATCGCACTGCT+TGG 0.544553 8_4:-44883701 None:intergenic
AGAAGTATTATGGGTTGGCT+AGG 0.551256 8_4:+44883532 Msa1327510:CDS
GGAAATATACAATGAGTGCC+AGG 0.561864 8_4:-44882722 None:intergenic
CACAAACCAAGCAGTGCGAT+GGG 0.562671 8_4:+44883695 Msa1327510:CDS
CGCCTAGCATGCAACGCATG+TGG 0.562848 8_4:-44882978 None:intergenic
CAACTTGTAATGCCATATGT+GGG 0.566377 8_4:-44885339 None:intergenic
CTCTTCTAAGGCAGTGAGAG+TGG 0.571123 8_4:-44883494 None:intergenic
AAGGAGTACTAAGTTCCTGC+AGG 0.571589 8_4:-44885266 None:intergenic
TACCTTCACATGTGGTCCAG+TGG 0.571838 8_4:+44885121 Msa1327510:three_prime_UTR
CTGCAAGAAGTATTATGGGT+TGG 0.577075 8_4:+44883527 Msa1327510:CDS
TCAAGTATTGACACTACAGC+GGG 0.577354 8_4:+44883925 Msa1327510:CDS
CTCCACTGGACCACATGTGA+AGG 0.579062 8_4:-44885123 None:intergenic
ACATTAGGTGGACCGAAAGT+TGG 0.579207 8_4:+44883257 Msa1327510:intron
GTCTATGGAGTGACTTACAT+CGG 0.591460 8_4:+44883303 Msa1327510:CDS
GTCTCCTCGTTCCGGTAAAG+GGG 0.591775 8_4:-44885020 None:intergenic
GTACAACCTTACCGAGAAGT+AGG 0.593326 8_4:+44883741 Msa1327510:CDS
CTGTAGTGTCAATACTTGAA+GGG 0.597211 8_4:-44883921 None:intergenic
CAGATTTCTAGTTCCAAACA+AGG 0.599407 8_4:-44885285 None:intergenic
GACACATGCATGCGACGTTG+AGG 0.600599 8_4:+44884376 Msa1327510:CDS
GTAAGATATATACTTGCATG+GGG 0.616463 8_4:+44885169 Msa1327510:three_prime_UTR
CCGAGAAGTAGGAAGACACC+TGG 0.620898 8_4:+44883752 Msa1327510:CDS
CAGTTGCTTGTAAACAAGCA+GGG 0.625484 8_4:+44884227 Msa1327510:CDS
GTTGTACAACTGGAAGCCCA+AGG 0.625869 8_4:-44883725 None:intergenic
TCTCCTCGTTCCGGTAAAGG+GGG 0.625906 8_4:-44885019 None:intergenic
TTCAAGTATTGACACTACAG+CGG 0.629318 8_4:+44883924 Msa1327510:CDS
TTGTACAACTGGAAGCCCAA+GGG 0.635369 8_4:-44883724 None:intergenic
GGAAATGAACAGGATACTCA+GGG 0.637226 8_4:+44884397 Msa1327510:CDS
AAGAGAGTGAACAAAATTTG+GGG 0.638223 8_4:-44884179 None:intergenic
AGTCTATGTTACACCAACCA+TGG 0.640712 8_4:-44885426 None:intergenic
GAGGCTTAAGGAGCGTTGTG+CGG 0.659755 8_4:+44883347 Msa1327510:CDS
ACTACAGCGGGAGACTGACA+AGG 0.666691 8_4:+44883937 Msa1327510:CDS
AACTTGTAATGCCATATGTG+GGG 0.667167 8_4:-44885338 None:intergenic
CCCGAGACCAGATCAAAAGG+AGG 0.677771 8_4:+44883328 Msa1327510:CDS
TGTACAACTGGAAGCCCAAG+GGG 0.678513 8_4:-44883723 None:intergenic
TCTAGTACTACCTTCACATG+TGG 0.682361 8_4:+44885113 Msa1327510:three_prime_UTR
TTGGCTAGGTGATTGTGCAA+AGG 0.687857 8_4:+44883546 Msa1327510:CDS
AGAGAGTGAACAAAATTTGG+GGG 0.694041 8_4:-44884178 None:intergenic
CTATGGAGTGACTTACATCG+GGG 0.760912 8_4:+44883305 Msa1327510:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTATTTTAAATTTTTGTAA+TGG + chr8_4:44883051-44883070 Msa1327510:intron 10.0%
!!! CTTTAATTTAAACTAAAATT+GGG - chr8_4:44884103-44884122 None:intergenic 10.0%
!!! TCTTTAATTTAAACTAAAAT+TGG - chr8_4:44884104-44884123 None:intergenic 10.0%
!!! TCTGATAATATTATTTTTAT+TGG - chr8_4:44885689-44885708 None:intergenic 10.0%
!! AATATTATGATTAGGATAAA+GGG - chr8_4:44882768-44882787 None:intergenic 15.0%
!! TAATATTATGATTAGGATAA+AGG - chr8_4:44882769-44882788 None:intergenic 15.0%
!! TGAAAGTTAATATTATGATT+AGG - chr8_4:44882776-44882795 None:intergenic 15.0%
!! AATATACTATTCATAGATAA+AGG - chr8_4:44882801-44882820 None:intergenic 15.0%
!! AAGTAAGAGAAATTAATATA+TGG + chr8_4:44883415-44883434 Msa1327510:intron 15.0%
!! AAACAACAAAAGTAAAATTT+AGG - chr8_4:44883796-44883815 None:intergenic 15.0%
!!! AATTATTTTAGTCACTAATA+TGG + chr8_4:44883869-44883888 Msa1327510:intron 15.0%
!! AATATTCAAATCTAATTACA+TGG - chr8_4:44883991-44884010 None:intergenic 15.0%
!!! GTATTTTATTTCATTCTTTT+TGG + chr8_4:44885548-44885567 Msa1327510:three_prime_UTR 15.0%
!!! GTATAAGTTATTTCAAATAA+TGG - chr8_4:44885651-44885670 None:intergenic 15.0%
!! AAAAAAAAAATAGAACCAGA+GGG + chr8_4:44883088-44883107 Msa1327510:intron 20.0%
!! TTAATATATGGAGTTAAATG+TGG + chr8_4:44883427-44883446 Msa1327510:intron 20.0%
!!! TACTTTACATTTTCCAATAT+CGG - chr8_4:44884449-44884468 None:intergenic 20.0%
!!! AATGTAAAGTATCTTCATTT+AGG + chr8_4:44884458-44884477 Msa1327510:intron 20.0%
!!! ATTACAAGTTGATTTTGAAT+TGG + chr8_4:44885351-44885370 Msa1327510:three_prime_UTR 20.0%
!! TGTAACATAGACTAATAAAA+AGG + chr8_4:44885436-44885455 Msa1327510:three_prime_UTR 20.0%
! GAAAAAAAAAATAGAACCAG+AGG + chr8_4:44883087-44883106 Msa1327510:intron 25.0%
! AATGTGCTTTCATATCATAA+AGG - chr8_4:44883183-44883202 None:intergenic 25.0%
! TATTCATAACTGACAACATT+AGG + chr8_4:44883242-44883261 Msa1327510:intron 25.0%
! TAGTCACTAATATGGAAAAT+TGG + chr8_4:44883877-44883896 Msa1327510:intron 25.0%
! CTAATATGGAAAATTGGATT+TGG + chr8_4:44883883-44883902 Msa1327510:intron 25.0%
! TTCAAATCTAATTACATGGT+AGG - chr8_4:44883987-44884006 None:intergenic 25.0%
!! TTTGTGTTCTTGTTTGTATT+AGG + chr8_4:44884300-44884319 Msa1327510:intron 25.0%
! TAAATCAATGCTTAATTGCA+GGG + chr8_4:44884336-44884355 Msa1327510:intron 25.0%
!!! TTTCCTTATTTTCTGGTATT+GGG + chr8_4:44884535-44884554 Msa1327510:intron 25.0%
! ATGCCTTAAAATTAAGTACA+TGG + chr8_4:44884564-44884583 Msa1327510:intron 25.0%
!! TGTCCATGTACTTAATTTTA+AGG - chr8_4:44884570-44884589 None:intergenic 25.0%
! TCAAATAAGTCAATTCAAAC+AGG - chr8_4:44884743-44884762 None:intergenic 25.0%
! AATAAGATGAAAACAGCTTA+TGG - chr8_4:44884822-44884841 None:intergenic 25.0%
!!! TTACAGCTTGTTGTATTTTT+TGG + chr8_4:44884877-44884896 Msa1327510:intron 25.0%
! TCTTACAAATTTCATCACAT+TGG - chr8_4:44885155-44885174 None:intergenic 25.0%
! TTGTAAGATATATACTTGCA+TGG + chr8_4:44885167-44885186 Msa1327510:three_prime_UTR 25.0%
! TGTAAGATATATACTTGCAT+GGG + chr8_4:44885168-44885187 Msa1327510:three_prime_UTR 25.0%
!! AGTGTTCAAAGCAAATATTT+TGG + chr8_4:44885228-44885247 Msa1327510:three_prime_UTR 25.0%
!!! ATTTCATTCTTTTTGGTTTG+TGG + chr8_4:44885555-44885574 Msa1327510:three_prime_UTR 25.0%
!!! ATATGTAGCATTTTTTGTCA+TGG + chr8_4:44885595-44885614 Msa1327510:three_prime_UTR 25.0%
! ATGCATCTCTTTTCATGATT+TGG - chr8_4:44882938-44882957 None:intergenic 30.0%
CTTGAAAGTAAAAAGAAACC+TGG - chr8_4:44883013-44883032 None:intergenic 30.0%
TTCATGTGTTGTATATGAAG+TGG + chr8_4:44883136-44883155 Msa1327510:intron 30.0%
TTGATATAAGAATAAGCCTG+AGG - chr8_4:44883216-44883235 None:intergenic 30.0%
!! TGATTATGCTTTGACTTGTA+TGG + chr8_4:44883619-44883638 Msa1327510:intron 30.0%
TTCTACAAGCCAAATAATTC+AGG + chr8_4:44883834-44883853 Msa1327510:intron 30.0%
TAAACTGCACCTGAATTATT+TGG - chr8_4:44883846-44883865 None:intergenic 30.0%
! ATTGGACTCATTCGTTTTAA+TGG + chr8_4:44884062-44884081 Msa1327510:intron 30.0%
ATATTTCATACACACGTTAC+AGG + chr8_4:44884126-44884145 Msa1327510:intron 30.0%
AAGAGAGTGAACAAAATTTG+GGG - chr8_4:44884182-44884201 None:intergenic 30.0%
CAAGAGAGTGAACAAAATTT+GGG - chr8_4:44884183-44884202 None:intergenic 30.0%
TCAAGAGAGTGAACAAAATT+TGG - chr8_4:44884184-44884203 None:intergenic 30.0%
! AATTTTGTTCACTCTCTTGA+TGG + chr8_4:44884184-44884203 Msa1327510:CDS 30.0%
ACAAGAACACAAATACAAGA+CGG - chr8_4:44884293-44884312 None:intergenic 30.0%
GTAAATCAATGCTTAATTGC+AGG + chr8_4:44884335-44884354 Msa1327510:intron 30.0%
AAATCAATGCTTAATTGCAG+GGG + chr8_4:44884337-44884356 Msa1327510:intron 30.0%
TAAATGCCACAATATAATCC+TGG + chr8_4:44884502-44884521 Msa1327510:intron 30.0%
CAATACCAGAAAATAAGGAA+AGG - chr8_4:44884536-44884555 None:intergenic 30.0%
!!! CTTTCCTTATTTTCTGGTAT+TGG + chr8_4:44884534-44884553 Msa1327510:intron 30.0%
TAACCCAATACCAGAAAATA+AGG - chr8_4:44884541-44884560 None:intergenic 30.0%
AAGACCTATCTTTGAATTCT+TGG + chr8_4:44884611-44884630 Msa1327510:intron 30.0%
AATGCCAAGAATTCAAAGAT+AGG - chr8_4:44884618-44884637 None:intergenic 30.0%
ACTTATTTGAGCTTATCTAC+CGG + chr8_4:44884753-44884772 Msa1327510:intron 30.0%
GCAGTCTAGTTGAAAAAATA+GGG - chr8_4:44885073-44885092 None:intergenic 30.0%
TGCAGTCTAGTTGAAAAAAT+AGG - chr8_4:44885074-44885093 None:intergenic 30.0%
GTAAGATATATACTTGCATG+GGG + chr8_4:44885169-44885188 Msa1327510:three_prime_UTR 30.0%
AGGATTGAAGTGATCATATA+AGG + chr8_4:44885456-44885475 Msa1327510:three_prime_UTR 30.0%
! TAAGGTGATTTTCTCAGATA+TGG + chr8_4:44885474-44885493 Msa1327510:three_prime_UTR 30.0%
ACAAAAAGCAAAACCCAATT+TGG - chr8_4:44885521-44885540 None:intergenic 30.0%
! AGTAGCGCTAGAACAAATTT+TGG - chr8_4:44882874-44882893 None:intergenic 35.0%
TCATAACTGACAACATTAGG+TGG + chr8_4:44883245-44883264 Msa1327510:intron 35.0%
!! TCTTACTTTTGCAGCATAAC+AGG - chr8_4:44883403-44883422 None:intergenic 35.0%
!!! TAATGGTTGAGTTTTGAACG+TGG + chr8_4:44883656-44883675 Msa1327510:intron 35.0%
!! CTTCTGTAAAGAAAAGTACC+AGG - chr8_4:44883773-44883792 None:intergenic 35.0%
CTGTAGTGTCAATACTTGAA+GGG - chr8_4:44883924-44883943 None:intergenic 35.0%
!! TTCAAGTATTGACACTACAG+CGG + chr8_4:44883924-44883943 Msa1327510:CDS 35.0%
AGAGAGTGAACAAAATTTGG+GGG - chr8_4:44884181-44884200 None:intergenic 35.0%
CCACAATATAATCCTGGTTT+GGG + chr8_4:44884508-44884527 Msa1327510:intron 35.0%
GACTTGATGTGATATAACAC+AGG - chr8_4:44884680-44884699 None:intergenic 35.0%
! TCAAGTCAAACTCCTTTTTG+TGG + chr8_4:44884693-44884712 Msa1327510:intron 35.0%
! CAAGTCAAACTCCTTTTTGT+GGG + chr8_4:44884694-44884713 Msa1327510:intron 35.0%
TACAAGGAAATCCCACAAAA+AGG - chr8_4:44884708-44884727 None:intergenic 35.0%
GATTTCCTTGTACTGAACTT+AGG + chr8_4:44884716-44884735 Msa1327510:intron 35.0%
! CTGATCACTAACCTTTTCAT+TGG + chr8_4:44884941-44884960 Msa1327510:intron 35.0%
GCATTTGGTATCCAATGAAA+AGG - chr8_4:44884955-44884974 None:intergenic 35.0%
AATTCTCCAACAACTGCATT+TGG - chr8_4:44884970-44884989 None:intergenic 35.0%
AAAGGGGGAAACTTCAAATT+GGG - chr8_4:44885007-44885026 None:intergenic 35.0%
TAAAGGGGGAAACTTCAAAT+TGG - chr8_4:44885008-44885027 None:intergenic 35.0%
!! CAGATTTCTAGTTCCAAACA+AGG - chr8_4:44885288-44885307 None:intergenic 35.0%
! CTTGTTTGGAACTAGAAATC+TGG + chr8_4:44885286-44885305 Msa1327510:three_prime_UTR 35.0%
AACTTGTAATGCCATATGTG+GGG - chr8_4:44885341-44885360 None:intergenic 35.0%
CAACTTGTAATGCCATATGT+GGG - chr8_4:44885342-44885361 None:intergenic 35.0%
TCAACTTGTAATGCCATATG+TGG - chr8_4:44885343-44885362 None:intergenic 35.0%
!!! AAGCCAATCTGTTTTTTCCA+TGG + chr8_4:44885409-44885428 Msa1327510:three_prime_UTR 35.0%
CAACCATGGAAAAAACAGAT+TGG - chr8_4:44885415-44885434 None:intergenic 35.0%
!!! CAATCTGTTTTTTCCATGGT+TGG + chr8_4:44885413-44885432 Msa1327510:three_prime_UTR 35.0%
TCTTGTGAAGCAACCAAATT+GGG + chr8_4:44885505-44885524 Msa1327510:three_prime_UTR 35.0%
!!! ATTCTTTTTGGTTTGTGGAG+AGG + chr8_4:44885560-44885579 Msa1327510:three_prime_UTR 35.0%
!!! TTCTTTTTGGTTTGTGGAGA+GGG + chr8_4:44885561-44885580 Msa1327510:three_prime_UTR 35.0%
GGAAATATACAATGAGTGCC+AGG - chr8_4:44882725-44882744 None:intergenic 40.0%
!!! GTCATGTGAGTCACTTTTTC+GGG - chr8_4:44882746-44882765 None:intergenic 40.0%
!!! GGTCATGTGAGTCACTTTTT+CGG - chr8_4:44882747-44882766 None:intergenic 40.0%
!! CATAGCTCTAAAAATCCCTC+TGG - chr8_4:44883106-44883125 None:intergenic 40.0%
!!! CAGAGGGATTTTTAGAGCTA+TGG + chr8_4:44883104-44883123 Msa1327510:intron 40.0%
CACATTCATTTGTCAACCTC+AGG + chr8_4:44883197-44883216 Msa1327510:intron 40.0%
CTGTCTGCTTTACCAACTTT+CGG - chr8_4:44883272-44883291 None:intergenic 40.0%
GTCTATGGAGTGACTTACAT+CGG + chr8_4:44883303-44883322 Msa1327510:CDS 40.0%
TCTATGGAGTGACTTACATC+GGG + chr8_4:44883304-44883323 Msa1327510:CDS 40.0%
AGTGAGAGTGGTCTAAACAA+TGG - chr8_4:44883485-44883504 None:intergenic 40.0%
TGCCTTAGAAGAGATGTTTG+AGG + chr8_4:44883504-44883523 Msa1327510:CDS 40.0%
AGCCTCAAACATCTCTTCTA+AGG - chr8_4:44883509-44883528 None:intergenic 40.0%
!! TGAGGCTGCAAGAAGTATTA+TGG + chr8_4:44883522-44883541 Msa1327510:CDS 40.0%
!! GAGGCTGCAAGAAGTATTAT+GGG + chr8_4:44883523-44883542 Msa1327510:CDS 40.0%
!! CTGCAAGAAGTATTATGGGT+TGG + chr8_4:44883527-44883546 Msa1327510:CDS 40.0%
!! AGAAGTATTATGGGTTGGCT+AGG + chr8_4:44883532-44883551 Msa1327510:CDS 40.0%
TGGCATCTTCGTCATTGTAA+TGG + chr8_4:44883639-44883658 Msa1327510:intron 40.0%
!!! TTGAGTTTTGAACGTGGAGT+AGG + chr8_4:44883662-44883681 Msa1327510:intron 40.0%
GCTGTAGTGTCAATACTTGA+AGG - chr8_4:44883925-44883944 None:intergenic 40.0%
!! TCAAGTATTGACACTACAGC+GGG + chr8_4:44883925-44883944 Msa1327510:CDS 40.0%
ATGATTATGCATCACAGCGT+AGG + chr8_4:44884039-44884058 Msa1327510:intron 40.0%
CGTTACAGGTCATGGTAAAA+AGG + chr8_4:44884140-44884159 Msa1327510:intron 40.0%
CAGTTGCTTGTAAACAAGCA+GGG + chr8_4:44884227-44884246 Msa1327510:CDS 40.0%
AGGAAATGAACAGGATACTC+AGG + chr8_4:44884396-44884415 Msa1327510:CDS 40.0%
GGAAATGAACAGGATACTCA+GGG + chr8_4:44884397-44884416 Msa1327510:CDS 40.0%
ACTCTACGAAACTCCGATAT+TGG + chr8_4:44884433-44884452 Msa1327510:CDS 40.0%
GCCACAATATAATCCTGGTT+TGG + chr8_4:44884507-44884526 Msa1327510:intron 40.0%
CCCAAACCAGGATTATATTG+TGG - chr8_4:44884511-44884530 None:intergenic 40.0%
!!! GGGTTCCTTTCCTTATTTTC+TGG + chr8_4:44884528-44884547 Msa1327510:intron 40.0%
CAAAGATAGGTCTTCATCCA+TGG - chr8_4:44884605-44884624 None:intergenic 40.0%
TAACACAGGTGTGTATCAAC+AGG - chr8_4:44884666-44884685 None:intergenic 40.0%
TGGATACCAAATGCAGTTGT+TGG + chr8_4:44884961-44884980 Msa1327510:intron 40.0%
! TTTGAAGTTTCCCCCTTTAC+CGG + chr8_4:44885009-44885028 Msa1327510:CDS 40.0%
TCTAGTACTACCTTCACATG+TGG + chr8_4:44885113-44885132 Msa1327510:three_prime_UTR 40.0%
GGAACTTAGTACTCCTTGTT+TGG + chr8_4:44885272-44885291 Msa1327510:three_prime_UTR 40.0%
AGTCTATGTTACACCAACCA+TGG - chr8_4:44885429-44885448 None:intergenic 40.0%
CTCTTGTGAAGCAACCAAAT+TGG + chr8_4:44885504-44885523 Msa1327510:three_prime_UTR 40.0%
ACATTAGGTGGACCGAAAGT+TGG + chr8_4:44883257-44883276 Msa1327510:intron 45.0%
! ACAGTGATGACGTCAGTCTA+TGG + chr8_4:44883288-44883307 Msa1327510:CDS 45.0%
CTATGGAGTGACTTACATCG+GGG + chr8_4:44883305-44883324 Msa1327510:CDS 45.0%
! CCTTAAGCCTCCTTTTGATC+TGG - chr8_4:44883338-44883357 None:intergenic 45.0%
CCAGATCAAAAGGAGGCTTA+AGG + chr8_4:44883335-44883354 Msa1327510:CDS 45.0%
TTGGCTAGGTGATTGTGCAA+AGG + chr8_4:44883546-44883565 Msa1327510:CDS 45.0%
TTGTACAACTGGAAGCCCAA+GGG - chr8_4:44883727-44883746 None:intergenic 45.0%
TCTCGGTAAGGTTGTACAAC+TGG - chr8_4:44883738-44883757 None:intergenic 45.0%
GTACAACCTTACCGAGAAGT+AGG + chr8_4:44883741-44883760 Msa1327510:CDS 45.0%
TGTCTTCCTACTTCTCGGTA+AGG - chr8_4:44883750-44883769 None:intergenic 45.0%
TATGCATCACAGCGTAGGAT+TGG + chr8_4:44884044-44884063 Msa1327510:intron 45.0%
CATACACACGTTACAGGTCA+TGG + chr8_4:44884132-44884151 Msa1327510:intron 45.0%
GCAGTTGCTTGTAAACAAGC+AGG + chr8_4:44884226-44884245 Msa1327510:CDS 45.0%
! CAGGGGTGCATGATTCTTAT+TGG + chr8_4:44884354-44884373 Msa1327510:intron 45.0%
TAAGGAAAGGAACCCAAACC+AGG - chr8_4:44884523-44884542 None:intergenic 45.0%
TCTTCATCCATGGCAATGCA+AGG - chr8_4:44884595-44884614 None:intergenic 45.0%
CAGGTCCTAAGTTCAGTACA+AGG - chr8_4:44884724-44884743 None:intergenic 45.0%
!! CAGCATGAAAGTGCTTATGC+CGG - chr8_4:44884775-44884794 None:intergenic 45.0%
CACATTGGAGTTAACTCCAC+TGG - chr8_4:44885140-44885159 None:intergenic 45.0%
! TTGCCATATTAGTCTCCTGC+AGG + chr8_4:44885251-44885270 Msa1327510:three_prime_UTR 45.0%
GTTCCTGCAGGAGACTAATA+TGG - chr8_4:44885257-44885276 None:intergenic 45.0%
!! AAGGAGTACTAAGTTCCTGC+AGG - chr8_4:44885269-44885288 None:intergenic 45.0%
!! GGGGCACTCTTTCTTTTCAA+TGG - chr8_4:44885322-44885341 None:intergenic 45.0%
!! AAGAAAGAGTGCCCCACATA+TGG + chr8_4:44885327-44885346 Msa1327510:three_prime_UTR 45.0%
!! TTGGTTTGTGGAGAGGGAAA+TGG + chr8_4:44885567-44885586 Msa1327510:three_prime_UTR 45.0%
!! ATTTAACTAAATAAAAAAAA+AGG - chr8_4:44884487-44884506 None:intergenic 5.0%
AACGCATGTGGATCAGTTGC+AGG - chr8_4:44882969-44882988 None:intergenic 50.0%
ATCCACATGCGTTGCATGCT+AGG + chr8_4:44882976-44882995 Msa1327510:CDS 50.0%
TGCTAGGCGTTTGATCAACC+AGG + chr8_4:44882992-44883011 Msa1327510:CDS 50.0%
! CCTCCTTTTGATCTGGTCTC+GGG - chr8_4:44883331-44883350 None:intergenic 50.0%
! GCCTCCTTTTGATCTGGTCT+CGG - chr8_4:44883332-44883351 None:intergenic 50.0%
!! CTCTTCTAAGGCAGTGAGAG+TGG - chr8_4:44883497-44883516 None:intergenic 50.0%
CACAAACCAAGCAGTGCGAT+GGG + chr8_4:44883695-44883714 Msa1327510:CDS 50.0%
TGTACAACTGGAAGCCCAAG+GGG - chr8_4:44883726-44883745 None:intergenic 50.0%
GTTGTACAACTGGAAGCCCA+AGG - chr8_4:44883728-44883747 None:intergenic 50.0%
CCAGGTGTCTTCCTACTTCT+CGG - chr8_4:44883755-44883774 None:intergenic 50.0%
CAAGCAGGGATGAACTTTGC+AGG + chr8_4:44884241-44884260 Msa1327510:CDS 50.0%
GCGACGTTGAGGAAATGAAC+AGG + chr8_4:44884387-44884406 Msa1327510:CDS 50.0%
GGACATACCTTGCATTGCCA+TGG + chr8_4:44884585-44884604 Msa1327510:intron 50.0%
AGTCTCCTCGTTCCGGTAAA+GGG - chr8_4:44885024-44885043 None:intergenic 50.0%
AAGTCTCCTCGTTCCGGTAA+AGG - chr8_4:44885025-44885044 None:intergenic 50.0%
TTTACCGGAACGAGGAGACT+TGG + chr8_4:44885024-44885043 Msa1327510:CDS 50.0%
AGATCCAAGTCTCCTCGTTC+CGG - chr8_4:44885031-44885050 None:intergenic 50.0%
TACCTTCACATGTGGTCCAG+TGG + chr8_4:44885121-44885140 Msa1327510:three_prime_UTR 50.0%
CCCGAGACCAGATCAAAAGG+AGG + chr8_4:44883328-44883347 Msa1327510:CDS 55.0%
!! GAGGCTTAAGGAGCGTTGTG+CGG + chr8_4:44883347-44883366 Msa1327510:CDS 55.0%
!! TAAGGAGCGTTGTGCGGTTG+AGG + chr8_4:44883353-44883372 Msa1327510:CDS 55.0%
!! TCGCACTGCTTGGTTTGTGC+AGG - chr8_4:44883694-44883713 None:intergenic 55.0%
GCACAAACCAAGCAGTGCGA+TGG + chr8_4:44883694-44883713 Msa1327510:CDS 55.0%
CCGAGAAGTAGGAAGACACC+TGG + chr8_4:44883752-44883771 Msa1327510:CDS 55.0%
ACTACAGCGGGAGACTGACA+AGG + chr8_4:44883937-44883956 Msa1327510:CDS 55.0%
GACACATGCATGCGACGTTG+AGG + chr8_4:44884376-44884395 Msa1327510:CDS 55.0%
TTTCCCCCTTTACCGGAACG+AGG + chr8_4:44885016-44885035 Msa1327510:CDS 55.0%
TCTCCTCGTTCCGGTAAAGG+GGG - chr8_4:44885022-44885041 None:intergenic 55.0%
GTCTCCTCGTTCCGGTAAAG+GGG - chr8_4:44885023-44885042 None:intergenic 55.0%
CTCCACTGGACCACATGTGA+AGG - chr8_4:44885126-44885145 None:intergenic 55.0%
CGCCTAGCATGCAACGCATG+TGG - chr8_4:44882981-44883000 None:intergenic 60.0%
GGGCCCGAGACCAGATCAAA+AGG + chr8_4:44883325-44883344 Msa1327510:CDS 60.0%
GAGTGACCCATCGCACTGCT+TGG - chr8_4:44883704-44883723 None:intergenic 60.0%
TGCGATGGGTCACTCCCCTT+GGG + chr8_4:44883709-44883728 Msa1327510:CDS 60.0%
GTGCGATGGGTCACTCCCCT+TGG + chr8_4:44883708-44883727 Msa1327510:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 44882719 44885713 44882719 ID=Msa1327510;Name=Msa1327510
chr8_4 mRNA 44882719 44885713 44882719 ID=Msa1327510-mRNA-1;Parent=Msa1327510;Name=Msa1327510-mRNA-1;_AED=0.00;_eAED=0.00;_QI=223|1|1|1|1|1|8|626|255
chr8_4 exon 44882719 44883013 44882719 ID=Msa1327510-mRNA-1:exon:12482;Parent=Msa1327510-mRNA-1
chr8_4 exon 44883264 44883416 44883264 ID=Msa1327510-mRNA-1:exon:12483;Parent=Msa1327510-mRNA-1
chr8_4 exon 44883493 44883567 44883493 ID=Msa1327510-mRNA-1:exon:12484;Parent=Msa1327510-mRNA-1
chr8_4 exon 44883684 44883773 44883684 ID=Msa1327510-mRNA-1:exon:12485;Parent=Msa1327510-mRNA-1
chr8_4 exon 44883911 44883958 44883911 ID=Msa1327510-mRNA-1:exon:12486;Parent=Msa1327510-mRNA-1
chr8_4 exon 44884148 44884262 44884148 ID=Msa1327510-mRNA-1:exon:12487;Parent=Msa1327510-mRNA-1
chr8_4 exon 44884357 44884460 44884357 ID=Msa1327510-mRNA-1:exon:12488;Parent=Msa1327510-mRNA-1
chr8_4 exon 44884977 44885713 44884977 ID=Msa1327510-mRNA-1:exon:12489;Parent=Msa1327510-mRNA-1
chr8_4 five_prime_UTR 44882719 44882941 44882719 ID=Msa1327510-mRNA-1:five_prime_utr;Parent=Msa1327510-mRNA-1
chr8_4 CDS 44882942 44883013 44882942 ID=Msa1327510-mRNA-1:cds;Parent=Msa1327510-mRNA-1
chr8_4 CDS 44883264 44883416 44883264 ID=Msa1327510-mRNA-1:cds;Parent=Msa1327510-mRNA-1
chr8_4 CDS 44883493 44883567 44883493 ID=Msa1327510-mRNA-1:cds;Parent=Msa1327510-mRNA-1
chr8_4 CDS 44883684 44883773 44883684 ID=Msa1327510-mRNA-1:cds;Parent=Msa1327510-mRNA-1
chr8_4 CDS 44883911 44883958 44883911 ID=Msa1327510-mRNA-1:cds;Parent=Msa1327510-mRNA-1
chr8_4 CDS 44884148 44884262 44884148 ID=Msa1327510-mRNA-1:cds;Parent=Msa1327510-mRNA-1
chr8_4 CDS 44884357 44884460 44884357 ID=Msa1327510-mRNA-1:cds;Parent=Msa1327510-mRNA-1
chr8_4 CDS 44884977 44885087 44884977 ID=Msa1327510-mRNA-1:cds;Parent=Msa1327510-mRNA-1
chr8_4 three_prime_UTR 44885088 44885713 44885088 ID=Msa1327510-mRNA-1:three_prime_utr;Parent=Msa1327510-mRNA-1
Gene Sequence

>Msa1327510

ATGAAAAGAGATGCATTGAAAGATCCTGCAACTGATCCACATGCGTTGCATGCTAGGCGTTTGATCAACCAGGTGGACCGAAAGTTGGTAAAGCAGACAGTGATGACGTCAGTCTATGGAGTGACTTACATCGGGGCCCGAGACCAGATCAAAAGGAGGCTTAAGGAGCGTTGTGCGGTTGAGGATGATTCAGAACTATTTGCTGCATCCTGTTATGCTGCAAAAACCACTCTCACTGCCTTAGAAGAGATGTTTGAGGCTGCAAGAAGTATTATGGGTTGGCTAGGTGATTGTGCAAAGGTGATAGCCTGCACAAACCAAGCAGTGCGATGGGTCACTCCCCTTGGGCTTCCAGTTGTACAACCTTACCGAGAAGTAGGAAGACACCTGATCAAGACATCCCTTCAAGTATTGACACTACAGCGGGAGACTGACAAGGTCATGGTAAAAAGGCAGAGAACAGCTTTTCCCCCAAATTTTGTTCACTCTCTTGATGGTTCTCACATGATGATGACTGCAGTTGCTTGTAAACAAGCAGGGATGAACTTTGCAGGGGTGCATGATTCTTATTGGACACATGCATGCGACGTTGAGGAAATGAACAGGATACTCAGGGAAAAGTTTGTTGAACTCTACGAAACTCCGATATTGGAAAATTTGTTGGAGAATTTTGAAAAAACTTTTCCCAATTTGAAGTTTCCCCCTTTACCGGAACGAGGAGACTTGGATCTTCAAGAAGTTTTGAAGTCTCCCTATTTTTTCAACTAG
Protein sequence

>Msa1327510

MKRDALKDPATDPHALHARRLINQVDRKLVKQTVMTSVYGVTYIGARDQIKRRLKERCAVEDDSELFAASCYAAKTTLTALEEMFEAARSIMGWLGDCAKVIACTNQAVRWVTPLGLPVVQPYREVGRHLIKTSLQVLTLQRETDKVMVKRQRTAFPPNFVHSLDGSHMMMTAVACKQAGMNFAGVHDSYWTHACDVEEMNRILREKFVELYETPILENLLENFEKTFPNLKFPPLPERGDLDLQEVLKSPYFFN*