AlfalfaGEDB | Msa1399530

M. sativa cultivar zhongmu-4 / Msa1399530

KEGG: K02918
GO: -
PFAM: PF00831.26

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1399530	XP_013470470.1	97.521	121	3	0	1	121	1	121	3.29e-76	231

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1399530	sp\|Q9M5L0\|RL35_EUPES	91.597	119	10	0	2	120	4	122	7.30e-72	213

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1399530	I3T2Q3	97.521	121	3	0	1	121	1	121	1.57e-76	231

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0002750	Msa1399530	0.868579	5.877599e-66	-8.615850e-47
Msa0002790	Msa1399530	0.856136	3.944812e-62	-8.615850e-47
Msa0003780	Msa1399530	0.931111	6.112049e-94	-8.615850e-47
Msa0022970	Msa1399530	0.811977	5.344615e-51	-8.615850e-47
Msa0025730	Msa1399530	0.806896	6.582544e-50	-8.615850e-47
Msa0027260	Msa1399530	0.834261	3.320929e-56	-8.615850e-47
Msa0029270	Msa1399530	0.806674	7.335610e-50	-8.615850e-47
Msa0032090	Msa1399530	0.845133	4.910344e-59	-8.615850e-47
Msa0032260	Msa1399530	0.801534	8.606009e-49	-8.615850e-47
Msa0032430	Msa1399530	0.824833	6.515213e-54	-8.615850e-47
Msa0032580	Msa1399530	0.817120	3.888483e-52	-8.615850e-47
Msa0037250	Msa1399530	0.800093	1.694337e-48	-8.615850e-47
Msa0040690	Msa1399530	0.888169	7.491669e-73	-8.615850e-47
Msa0042280	Msa1399530	0.827502	1.509894e-54	-8.615850e-47
Msa0043660	Msa1399530	0.822564	2.214137e-53	-8.615850e-47
Msa0045730	Msa1399530	0.881072	3.251734e-70	-8.615850e-47
Msa0047160	Msa1399530	0.830385	3.025587e-55	-8.615850e-47
Msa0048180	Msa1399530	0.817117	3.894663e-52	-8.615850e-47
Msa0049370	Msa1399530	0.810420	1.162872e-50	-8.615850e-47
Msa0055180	Msa1399530	0.807186	5.716218e-50	-8.615850e-47
Msa0056470	Msa1399530	0.826123	3.223754e-54	-8.615850e-47
Msa0074320	Msa1399530	0.810749	9.875497e-51	-8.615850e-47
Msa0077000	Msa1399530	0.807021	6.194797e-50	-8.615850e-47
Msa0086750	Msa1399530	0.844012	9.835151e-59	-8.615850e-47
Msa0091410	Msa1399530	0.804293	2.315797e-49	-8.615850e-47
Msa0091880	Msa1399530	0.825430	4.708248e-54	-8.615850e-47
Msa0094470	Msa1399530	0.836729	7.888285e-57	-8.615850e-47
Msa0097580	Msa1399530	0.809898	1.507268e-50	-8.615850e-47
Msa0100740	Msa1399530	0.801598	8.350063e-49	-8.615850e-47
Msa0116130	Msa1399530	0.823219	1.558403e-53	-8.615850e-47
Msa0118710	Msa1399530	0.820496	6.652374e-53	-8.615850e-47
Msa1358250	Msa1399530	0.804495	2.102350e-49	-8.615850e-47
Msa1362790	Msa1399530	0.828885	7.009339e-55	-8.615850e-47
Msa1363330	Msa1399530	0.864966	8.309652e-65	-8.615850e-47
Msa1366180	Msa1399530	0.860782	1.624445e-63	-8.615850e-47
Msa1374120	Msa1399530	0.914642	1.509126e-84	-8.615850e-47
Msa1379760	Msa1399530	0.835849	1.320807e-56	-8.615850e-47
Msa1382620	Msa1399530	0.869000	4.295965e-66	-8.615850e-47
Msa1386600	Msa1399530	0.865113	7.470046e-65	-8.615850e-47
Msa1394290	Msa1399530	0.860072	2.664021e-63	-8.615850e-47
Msa1399530	Msa1410440	0.812496	4.117928e-51	-8.615850e-47
Msa1399530	Msa1413500	0.806984	6.306146e-50	-8.615850e-47
Msa1399530	Msa1427560	0.824454	8.000265e-54	-8.615850e-47
Msa1399530	Msa1432700	0.836828	7.443691e-57	-8.615850e-47
Msa1399530	Msa1433310	0.817014	4.108432e-52	-8.615850e-47
Msa1399530	Msa1437860	0.817949	2.528526e-52	-8.615850e-47
Msa1399530	Msa1448690	0.860799	1.605241e-63	-8.615850e-47
Msa1399530	Msa1456050	0.815551	8.723207e-52	-8.615850e-47
Msa1399530	Msa1457920	0.831704	1.435514e-55	-8.615850e-47
Msa0603320	Msa1399530	0.817837	2.680140e-52	-8.615850e-47
Msa0608320	Msa1399530	0.820815	5.620460e-53	-8.615850e-47
Msa0615440	Msa1399530	0.862535	4.730197e-64	-8.615850e-47
Msa0620890	Msa1399530	0.809566	1.775848e-50	-8.615850e-47
Msa0622750	Msa1399530	0.904062	1.816079e-79	-8.615850e-47
Msa0623200	Msa1399530	0.890090	1.349039e-73	-8.615850e-47
Msa0623790	Msa1399530	0.824600	7.392170e-54	-8.615850e-47
Msa0643560	Msa1399530	0.820876	5.441017e-53	-8.615850e-47
Msa0647500	Msa1399530	0.800809	1.210736e-48	-8.615850e-47
Msa0661300	Msa1399530	0.827269	1.717492e-54	-8.615850e-47
Msa0669520	Msa1399530	0.802351	5.846717e-49	-8.615850e-47
Msa0688850	Msa1399530	0.810827	9.496213e-51	-8.615850e-47
Msa0689260	Msa1399530	0.800536	1.376422e-48	-8.615850e-47
Msa0698490	Msa1399530	0.862620	4.455559e-64	-8.615850e-47
Msa0698660	Msa1399530	0.864273	1.368714e-64	-8.615850e-47
Msa0698780	Msa1399530	0.834257	3.328182e-56	-8.615850e-47
Msa0738100	Msa1399530	0.814613	1.409227e-51	-8.615850e-47
Msa0753140	Msa1399530	0.818670	1.736264e-52	-8.615850e-47
Msa0780490	Msa1399530	0.831284	1.820999e-55	-8.615850e-47
Msa0785130	Msa1399530	0.802801	4.722722e-49	-8.615850e-47
Msa0788850	Msa1399530	0.847583	1.054729e-59	-8.615850e-47
Msa0792760	Msa1399530	0.814961	1.180181e-51	-8.615850e-47
Msa0806880	Msa1399530	0.801935	7.118803e-49	-8.615850e-47
Msa0817540	Msa1399530	0.804370	2.232013e-49	-8.615850e-47
Msa0830540	Msa1399530	0.807400	5.149114e-50	-8.615850e-47
Msa0127050	Msa1399530	0.807814	4.206675e-50	-8.615850e-47
Msa0127310	Msa1399530	0.821167	4.663118e-53	-8.615850e-47
Msa0128860	Msa1399530	0.830763	2.445656e-55	-8.615850e-47
Msa0133540	Msa1399530	0.819951	8.870083e-53	-8.615850e-47
Msa0137550	Msa1399530	0.832557	8.830757e-56	-8.615850e-47
Msa0140820	Msa1399530	0.821622	3.660216e-53	-8.615850e-47
Msa0146340	Msa1399530	0.927026	2.083798e-91	-8.615850e-47
Msa0150300	Msa1399530	0.850451	1.684028e-60	-8.615850e-47
Msa0169310	Msa1399530	0.803102	4.091873e-49	-8.615850e-47
Msa0172070	Msa1399530	0.829732	4.367016e-55	-8.615850e-47
Msa0172970	Msa1399530	0.815195	1.047065e-51	-8.615850e-47
Msa0174730	Msa1399530	0.831250	1.856396e-55	-8.615850e-47
Msa0180020	Msa1399530	0.804621	1.978647e-49	-8.615850e-47
Msa0181490	Msa1399530	0.800260	1.566488e-48	-8.615850e-47
Msa0187690	Msa1399530	0.822750	2.004088e-53	-8.615850e-47
Msa0187930	Msa1399530	0.857792	1.281712e-62	-8.615850e-47
Msa0189300	Msa1399530	0.807915	4.003651e-50	-8.615850e-47
Msa0189700	Msa1399530	0.820592	6.324141e-53	-8.615850e-47
Msa0194540	Msa1399530	0.844985	5.385563e-59	-8.615850e-47
Msa0220720	Msa1399530	0.800119	1.673740e-48	-8.615850e-47
Msa0230490	Msa1399530	0.814644	1.386788e-51	-8.615850e-47
Msa0231660	Msa1399530	0.802430	5.631219e-49	-8.615850e-47
Msa0232010	Msa1399530	0.827136	1.848310e-54	-8.615850e-47
Msa0232080	Msa1399530	0.831219	1.889777e-55	-8.615850e-47
Msa1257510	Msa1399530	0.850415	1.723069e-60	-8.615850e-47
Msa1265630	Msa1399530	0.830689	2.549020e-55	-8.615850e-47
Msa1266060	Msa1399530	0.810224	1.282099e-50	-8.615850e-47
Msa1300380	Msa1399530	0.827150	1.833511e-54	-8.615850e-47
Msa1305050	Msa1399530	0.809420	1.908851e-50	-8.615850e-47
Msa1327510	Msa1399530	0.800408	1.461426e-48	-8.615850e-47
Msa1338190	Msa1399530	0.815252	1.017070e-51	-8.615850e-47
Msa1343260	Msa1399530	0.822598	2.174479e-53	-8.615850e-47
Msa0366890	Msa1399530	0.820614	6.248776e-53	-8.615850e-47
Msa0369450	Msa1399530	0.815377	9.538506e-52	-8.615850e-47
Msa0385580	Msa1399530	0.846528	2.052057e-59	-8.615850e-47
Msa0395890	Msa1399530	0.827756	1.311928e-54	-8.615850e-47
Msa0402650	Msa1399530	0.816442	5.519433e-52	-8.615850e-47
Msa0414800	Msa1399530	0.809246	2.079944e-50	-8.615850e-47
Msa0427150	Msa1399530	0.820238	7.624062e-53	-8.615850e-47
Msa0443390	Msa1399530	0.815279	1.002750e-51	-8.615850e-47
Msa0447220	Msa1399530	0.822732	2.024174e-53	-8.615850e-47
Msa0479430	Msa1399530	0.816256	6.075017e-52	-8.615850e-47
Msa0989540	Msa1399530	0.832351	9.931694e-56	-8.615850e-47
Msa1006840	Msa1399530	0.844616	6.771549e-59	-8.615850e-47
Msa1010310	Msa1399530	0.805672	1.192151e-49	-8.615850e-47
Msa1012740	Msa1399530	0.810506	1.114394e-50	-8.615850e-47
Msa1013400	Msa1399530	0.834593	2.740016e-56	-8.615850e-47
Msa1019240	Msa1399530	0.842375	2.686912e-58	-8.615850e-47
Msa1019260	Msa1399530	0.841820	3.768666e-58	-8.615850e-47
Msa1043690	Msa1399530	0.918482	1.476497e-86	-8.615850e-47
Msa1051880	Msa1399530	0.861573	9.329937e-64	-8.615850e-47
Msa1072710	Msa1399530	0.801678	8.038162e-49	-8.615850e-47
Msa1081150	Msa1399530	0.853860	1.807199e-61	-8.615850e-47
Msa1088490	Msa1399530	0.845572	3.736149e-59	-8.615850e-47
Msa1093530	Msa1399530	0.823365	1.440586e-53	-8.615850e-47
Msa1104140	Msa1399530	0.886515	3.197395e-72	-8.615850e-47
Msa1104210	Msa1399530	0.845529	3.837344e-59	-8.615850e-47
Msa0244780	Msa1399530	0.860475	2.012267e-63	-8.615850e-47
Msa0250820	Msa1399530	0.851320	9.582701e-61	-8.615850e-47
Msa0258850	Msa1399530	0.810947	8.947560e-51	-8.615850e-47
Msa0262310	Msa1399530	0.816850	4.470344e-52	-8.615850e-47
Msa0264010	Msa1399530	0.861356	1.086390e-63	-8.615850e-47
Msa0268130	Msa1399530	0.858847	6.219458e-63	-8.615850e-47
Msa0289580	Msa1399530	0.877563	5.683664e-69	-8.615850e-47
Msa0289610	Msa1399530	0.879315	1.377532e-69	-8.615850e-47
Msa0298140	Msa1399530	0.816386	5.679330e-52	-8.615850e-47
Msa0298200	Msa1399530	0.804566	2.031124e-49	-8.615850e-47
Msa0298990	Msa1399530	0.864835	9.136603e-65	-8.615850e-47
Msa0321640	Msa1399530	0.829266	5.667934e-55	-8.615850e-47
Msa0327300	Msa1399530	0.871611	5.989855e-67	-8.615850e-47
Msa0327330	Msa1399530	0.928016	5.235790e-92	-8.615850e-47
Msa0336010	Msa1399530	0.886485	3.282650e-72	-8.615850e-47
Msa0337650	Msa1399530	0.818885	1.551782e-52	-8.615850e-47
Msa0340380	Msa1399530	0.810364	1.195941e-50	-8.615850e-47
Msa0348830	Msa1399530	0.840808	6.954776e-58	-8.615850e-47
Msa1126510	Msa1399530	0.819884	9.184057e-53	-8.615850e-47
Msa1165940	Msa1399530	0.830602	2.677874e-55	-8.615850e-47
Msa1166860	Msa1399530	0.838545	2.699090e-57	-8.615850e-47
Msa1166870	Msa1399530	0.805196	1.500342e-49	-8.615850e-47
Msa1181830	Msa1399530	0.807231	5.591193e-50	-8.615850e-47
Msa1181850	Msa1399530	0.819315	1.239146e-52	-8.615850e-47
Msa1181860	Msa1399530	0.813189	2.903767e-51	-8.615850e-47
Msa1206670	Msa1399530	0.825002	5.942537e-54	-8.615850e-47
Msa1209200	Msa1399530	0.804001	2.663538e-49	-8.615850e-47
Msa1213480	Msa1399530	0.834076	3.693587e-56	-8.615850e-47
Msa1215820	Msa1399530	0.860993	1.401930e-63	-8.615850e-47
Msa1218600	Msa1399530	0.816919	4.314083e-52	-8.615850e-47
Msa0483110	Msa1399530	0.857099	2.054864e-62	-8.615850e-47
Msa0497490	Msa1399530	0.816276	6.009799e-52	-8.615850e-47
Msa0517050	Msa1399530	0.817056	4.019532e-52	-8.615850e-47
Msa0521050	Msa1399530	0.810272	1.251830e-50	-8.615850e-47
Msa0526540	Msa1399530	0.860636	1.798981e-63	-8.615850e-47
Msa0530820	Msa1399530	0.807881	4.069591e-50	-8.615850e-47
Msa0571920	Msa1399530	0.825340	4.945271e-54	-8.615850e-47
Msa0575170	Msa1399530	0.801435	9.015503e-49	-8.615850e-47
Msa0576940	Msa1399530	0.862814	3.882650e-64	-8.615850e-47
Msa0588360	Msa1399530	0.815937	7.157690e-52	-8.615850e-47
Msa0601150	Msa1399530	0.811593	6.478930e-51	-8.615850e-47
Msa0849500	Msa1399530	0.814302	1.651133e-51	-8.615850e-47
Msa0849510	Msa1399530	0.810934	9.004995e-51	-8.615850e-47
Msa0867060	Msa1399530	0.815830	7.562321e-52	-8.615850e-47
Msa0881290	Msa1399530	0.820718	5.916528e-53	-8.615850e-47
Msa0902760	Msa1399530	0.825716	4.026780e-54	-8.615850e-47
Msa0945990	Msa1399530	0.825795	3.857292e-54	-8.615850e-47
Msa0953310	Msa1399530	0.846574	1.993794e-59	-8.615850e-47
Msa0968590	Msa1399530	0.803209	3.888982e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa1399530	MtrunA17_Chr1g0212921	97.521	121	3	1	121	1	121	3.03e-80	231
Msa1399530	MtrunA17_Chr1g0182051	91.667	120	10	2	121	3	122	3.91e-74	216
Msa1399530	MtrunA17_Chr4g0030641	90.833	120	11	2	121	4	123	7.48e-72	210
Msa1399530	MtrunA17_Chr7g0249361	89.167	120	13	2	121	49	168	3.45e-70	207
Msa1399530	MtrunA17_Chr4g0036351	84.298	121	19	1	121	1	121	1.16e-66	197
Msa1399530	MtrunA17_Chr1g0206641	87.179	39	5	9	47	2	40	1.70e-15	65.5
Msa1399530	MtrunA17_Chr5g0419591	87.879	33	4	65	97	2	34	1.94e-13	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1399530	AT2G39390.1	87.395	119	15	0	2	120	4	122	1.75e-69	204
Msa1399530	AT3G09500.1	86.555	119	16	0	2	120	4	122	5.44e-68	201
Msa1399530	AT5G02610.1	86.555	119	16	0	2	120	4	122	7.55e-68	200
Msa1399530	AT3G55170.5	84.874	119	18	0	2	120	4	122	1.53e-66	197
Msa1399530	AT3G55170.2	84.874	119	18	0	2	120	4	122	1.53e-66	197
Msa1399530	AT3G55170.1	84.874	119	18	0	2	120	4	122	1.53e-66	197
Msa1399530	AT5G02610.2	72.535	142	16	1	2	120	4	145	3.50e-62	187
Msa1399530	AT3G55170.4	85.417	96	14	0	2	97	4	99	2.20e-51	158
Msa1399530	AT3G55170.3	85.417	96	14	0	2	97	4	99	2.20e-51	158

Find 51 sgRNAs with CRISPR-Local

Find 259 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTCTCTTCAATGTTTAAAAT+TGG	0.152270	tig0022368:-70096	Msa1399530:three_prime_UTR
CGAATGGCCCTGGTCTTCTT+TGG	0.171363	tig0022368:+71173	None:intergenic
TCAAAGCAGCATTCTGCTTC+TGG	0.230853	tig0022368:+71240	None:intergenic
AGAAAATAATGATAAATAAA+TGG	0.285104	tig0022368:+70055	None:intergenic
AATTTGCTTGGGGTATAATA+TGG	0.309101	tig0022368:-70032	Msa1399530:three_prime_UTR
CAAAACTTGTCAACTATTAG+AGG	0.334393	tig0022368:+70230	None:intergenic
TTGATAGCATATTTCCTCAT+TGG	0.349490	tig0022368:+70299	None:intergenic
GAATGGCCCTGGTCTTCTTT+GGG	0.358511	tig0022368:+71174	None:intergenic
GACCGAGAGGGAGAAGAAAA+AGG	0.368054	tig0022368:-70333	Msa1399530:CDS
TGATAGCATATTTCCTCATT+GGG	0.379576	tig0022368:+70300	None:intergenic
AGGGCATCATATCTATCTAT+TGG	0.403617	tig0022368:-70263	Msa1399530:three_prime_UTR
CTATCAAGGCATAGGGTTGT+AGG	0.404286	tig0022368:-70283	Msa1399530:exon
GTTTCCTTCCTGAAGCTACT+AGG	0.414193	tig0022368:-70155	Msa1399530:three_prime_UTR
CAAAGCAGCATTCTGCTTCT+GGG	0.419055	tig0022368:+71241	None:intergenic
GAGCCTTCTGCGAATGGCCC+TGG	0.419374	tig0022368:+71163	None:intergenic
AAACAGAATAAAGAAAATAC+AGG	0.435732	tig0022368:+70196	None:intergenic
CTCCGTGTTGCTAAGGTCAC+TGG	0.447113	tig0022368:-71638	Msa1399530:CDS
GAAGCAGAATGCTGCTTTGA+GGG	0.449593	tig0022368:-71237	Msa1399530:CDS
GTAAAGCAAGTTCAGCTTTG+AGG	0.452854	tig0022368:+71660	None:intergenic
CTTGGTGAGCCTTCTGCGAA+TGG	0.472202	tig0022368:+71157	None:intergenic
AATGAGGAAATATGCTATCA+AGG	0.472756	tig0022368:-70297	Msa1399530:CDS
TGCTTTACTCCGTGTTGCTA+AGG	0.473685	tig0022368:-71645	Msa1399530:CDS
ATATGGTCGATCTCACTTGA+AGG	0.487544	tig0022368:-70015	Msa1399530:three_prime_UTR
GAAATATGCTATCAAGGCAT+AGG	0.504384	tig0022368:-70291	Msa1399530:exon
AGTCTTGGTTCCTTTGTTAG+CGG	0.510513	tig0022368:+69959	None:intergenic
AGGGTTTAATACCTGGTGCT+TGG	0.515451	tig0022368:+71139	None:intergenic
CAGTGAAGGTTCACGAGTTG+AGG	0.518240	tig0022368:-71721	Msa1399530:CDS
ATCTCCGCCCAAAGAAGACC+AGG	0.531518	tig0022368:-71181	Msa1399530:CDS
GTCGATCTCACTTGAAGGGA+AGG	0.531578	tig0022368:-70010	Msa1399530:three_prime_UTR
CAGAAGGCTCACCAAGCACC+AGG	0.535404	tig0022368:-71150	Msa1399530:intron
CTGCAGAAGAATTCAACAAT+GGG	0.536601	tig0022368:-71839	Msa1399530:intron
TATCAAGGCATAGGGTTGTA+GGG	0.546897	tig0022368:-70282	Msa1399530:exon
TTCTTTGGGCGGAGATCAAG+TGG	0.553377	tig0022368:+71188	None:intergenic
AGAAGCAGAATGCTGCTTTG+AGG	0.568112	tig0022368:-71238	Msa1399530:CDS
GGTGAGGTTGTCGATTGCTC+AGG	0.570678	tig0022368:-71279	Msa1399530:CDS
TGGCCCTGGTCTTCTTTGGG+CGG	0.571866	tig0022368:+71177	None:intergenic
AAATATGCTATCAAGGCATA+GGG	0.572355	tig0022368:-70290	Msa1399530:exon
TATGGTCGATCTCACTTGAA+GGG	0.577623	tig0022368:-70014	Msa1399530:three_prime_UTR
AGACCAGGGCCATTCGCAGA+AGG	0.591558	tig0022368:-71166	Msa1399530:CDS
AACAGAATAAAGAAAATACA+GGG	0.606344	tig0022368:+70197	None:intergenic
AGGAGACATATTTCCCAATG+AGG	0.613250	tig0022368:-70313	Msa1399530:CDS
CACCAGTGACCTTAGCAACA+CGG	0.624056	tig0022368:+71636	None:intergenic
ACATTTAAAACCGCTAACAA+AGG	0.629922	tig0022368:-69969	Msa1399530:three_prime_UTR
GCTGCAGAAGAATTCAACAA+TGG	0.630743	tig0022368:-71840	Msa1399530:exon
TCGATCTCACTTGAAGGGAA+GGG	0.631667	tig0022368:-70009	Msa1399530:three_prime_UTR
AAATCCTAGTAGCTTCAGGA+AGG	0.634941	tig0022368:+70151	None:intergenic
GTCTTCGCTGAAGACCGAGA+GGG	0.684722	tig0022368:-70345	Msa1399530:CDS
CGTGTTGCTAAGGTCACTGG+TGG	0.692805	tig0022368:-71635	Msa1399530:CDS
TCTCCGCCCAAAGAAGACCA+GGG	0.718890	tig0022368:-71180	Msa1399530:CDS
AGTCTTCGCTGAAGACCGAG+AGG	0.727322	tig0022368:-70346	Msa1399530:CDS
ACAGAATAAAGAAAATACAG+GGG	0.741158	tig0022368:+70198	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTGAATTTTTATTTACATTT+TGG	+	tig0022368:70271-70290	None:intergenic	10.0%
!!!	TTGTTTTTGATTATTTTATT+TGG	-	tig0022368:70546-70565	Msa1399530:intron	10.0%
!!!	TGTTTTTGATTATTTTATTT+GGG	-	tig0022368:70547-70566	Msa1399530:intron	10.0%
!!	AGAAAATAATGATAAATAAA+TGG	+	tig0022368:71811-71830	None:intergenic	10.0%
!!!	TTGAATTTTTATTTACATTT+TGG	+	tig0022368:70271-70290	None:intergenic	10.0%
!!!	TTGTTTTTGATTATTTTATT+TGG	-	tig0022368:70546-70565	Msa1399530:intron	10.0%
!!!	TGTTTTTGATTATTTTATTT+GGG	-	tig0022368:70547-70566	Msa1399530:intron	10.0%
!!	AGAAAATAATGATAAATAAA+TGG	+	tig0022368:71811-71830	None:intergenic	10.0%
!!	ACATGAATTATACATTAAAA+GGG	+	tig0022368:70746-70765	None:intergenic	15.0%
!!	AACATGAATTATACATTAAA+AGG	+	tig0022368:70747-70766	None:intergenic	15.0%
!!	AAATTTCAGTTTGTATATAT+AGG	+	tig0022368:71420-71439	None:intergenic	15.0%
!!	ACATGAATTATACATTAAAA+GGG	+	tig0022368:70746-70765	None:intergenic	15.0%
!!	AACATGAATTATACATTAAA+AGG	+	tig0022368:70747-70766	None:intergenic	15.0%
!!	AAATTTCAGTTTGTATATAT+AGG	+	tig0022368:71420-71439	None:intergenic	15.0%
!!!	TTGTTTGATTTCAGAAATTA+GGG	+	tig0022368:70067-70086	None:intergenic	20.0%
!!!	TTTGTTTGATTTCAGAAATT+AGG	+	tig0022368:70068-70087	None:intergenic	20.0%
!!!	CTTTATTCTTTTATGTCAAT+TGG	-	tig0022368:70292-70311	Msa1399530:CDS	20.0%
!!	GAATGAAATCTTAAATAATG+AGG	-	tig0022368:70992-71011	Msa1399530:intron	20.0%
!!!	ACATTCAATTTTATGTGTTA+AGG	+	tig0022368:71178-71197	None:intergenic	20.0%
!!	TAGTGAATTTCATGTAAAAA+TGG	+	tig0022368:71207-71226	None:intergenic	20.0%
!!	TTAGAAGATAATAACTAGAT+TGG	-	tig0022368:71260-71279	Msa1399530:CDS	20.0%
!!	AACAGAATAAAGAAAATACA+GGG	+	tig0022368:71669-71688	None:intergenic	20.0%
!!	AAACAGAATAAAGAAAATAC+AGG	+	tig0022368:71670-71689	None:intergenic	20.0%
!!!	TCATTATTTTCTAATTTGCT+TGG	-	tig0022368:71819-71838	Msa1399530:intron	20.0%
!!!	CATTATTTTCTAATTTGCTT+GGG	-	tig0022368:71820-71839	Msa1399530:intron	20.0%
!!!	ATTATTTTCTAATTTGCTTG+GGG	-	tig0022368:71821-71840	Msa1399530:intron	20.0%
!!!	TTGTTTGATTTCAGAAATTA+GGG	+	tig0022368:70067-70086	None:intergenic	20.0%
!!!	TTTGTTTGATTTCAGAAATT+AGG	+	tig0022368:70068-70087	None:intergenic	20.0%
!!!	CTTTATTCTTTTATGTCAAT+TGG	-	tig0022368:70292-70311	Msa1399530:CDS	20.0%
!!	GAATGAAATCTTAAATAATG+AGG	-	tig0022368:70992-71011	Msa1399530:intron	20.0%
!!!	ACATTCAATTTTATGTGTTA+AGG	+	tig0022368:71178-71197	None:intergenic	20.0%
!!	TAGTGAATTTCATGTAAAAA+TGG	+	tig0022368:71207-71226	None:intergenic	20.0%
!!	TTAGAAGATAATAACTAGAT+TGG	-	tig0022368:71260-71279	Msa1399530:CDS	20.0%
!!	AACAGAATAAAGAAAATACA+GGG	+	tig0022368:71669-71688	None:intergenic	20.0%
!!	AAACAGAATAAAGAAAATAC+AGG	+	tig0022368:71670-71689	None:intergenic	20.0%
!!!	TCATTATTTTCTAATTTGCT+TGG	-	tig0022368:71819-71838	Msa1399530:intron	20.0%
!!!	CATTATTTTCTAATTTGCTT+GGG	-	tig0022368:71820-71839	Msa1399530:intron	20.0%
!!!	ATTATTTTCTAATTTGCTTG+GGG	-	tig0022368:71821-71840	Msa1399530:intron	20.0%
!	AACAATTTATCCTTCAGAAT+TGG	+	tig0022368:70428-70447	None:intergenic	25.0%
!	TGATTGTGTGATGATTATAA+TGG	-	tig0022368:70467-70486	Msa1399530:intron	25.0%
!	GATTGTGTGATGATTATAAT+GGG	-	tig0022368:70468-70487	Msa1399530:intron	25.0%
!!!	AGTTTTCTTTCTAAAATCGA+AGG	-	tig0022368:71091-71110	Msa1399530:intron	25.0%
!	CTTTCAAGGATAACATTTAA+TGG	+	tig0022368:71135-71154	None:intergenic	25.0%
!	TGATTATTACTGTTCTTTCA+AGG	+	tig0022368:71149-71168	None:intergenic	25.0%
!!!	TAACTAGATTGGTTTATTGT+TGG	-	tig0022368:71271-71290	Msa1399530:CDS	25.0%
!	ACAGAATAAAGAAAATACAG+GGG	+	tig0022368:71668-71687	None:intergenic	25.0%
!!	CATTTTTATCATACTCATTG+CGG	-	tig0022368:71739-71758	Msa1399530:intron	25.0%
!!	GTCTCTTCAATGTTTAAAAT+TGG	-	tig0022368:71767-71786	Msa1399530:intron	25.0%
!	AACAATTTATCCTTCAGAAT+TGG	+	tig0022368:70428-70447	None:intergenic	25.0%
!	TGATTGTGTGATGATTATAA+TGG	-	tig0022368:70467-70486	Msa1399530:intron	25.0%
!	GATTGTGTGATGATTATAAT+GGG	-	tig0022368:70468-70487	Msa1399530:intron	25.0%
!!!	AGTTTTCTTTCTAAAATCGA+AGG	-	tig0022368:71091-71110	Msa1399530:intron	25.0%
!	CTTTCAAGGATAACATTTAA+TGG	+	tig0022368:71135-71154	None:intergenic	25.0%
!	TGATTATTACTGTTCTTTCA+AGG	+	tig0022368:71149-71168	None:intergenic	25.0%
!!!	TAACTAGATTGGTTTATTGT+TGG	-	tig0022368:71271-71290	Msa1399530:CDS	25.0%
!	ACAGAATAAAGAAAATACAG+GGG	+	tig0022368:71668-71687	None:intergenic	25.0%
!!	CATTTTTATCATACTCATTG+CGG	-	tig0022368:71739-71758	Msa1399530:intron	25.0%
!!	GTCTCTTCAATGTTTAAAAT+TGG	-	tig0022368:71767-71786	Msa1399530:intron	25.0%
!!	TTATTTTGTAGTAAGCTGCT+AGG	-	tig0022368:69967-69986	Msa1399530:three_prime_UTR	30.0%
!	TATTTTGTAGTAAGCTGCTA+GGG	-	tig0022368:69968-69987	Msa1399530:three_prime_UTR	30.0%
!	TTTGATTTCAGAAATTAGGG+TGG	+	tig0022368:70064-70083	None:intergenic	30.0%
!!	GCACGATTGTTCTAAAAATA+AGG	+	tig0022368:70348-70367	None:intergenic	30.0%
	TATCCTTCAGAATTGGTAAA+TGG	+	tig0022368:70421-70440	None:intergenic	30.0%
!	GAAGGATAAATTGTTCCTTT+TGG	-	tig0022368:70433-70452	Msa1399530:intron	30.0%
!!	AATTGTTCCTTTTGGTGTAA+TGG	-	tig0022368:70441-70460	Msa1399530:intron	30.0%
!!!	GTTTTTGTTTTGTGACTGTA+AGG	-	tig0022368:70493-70512	Msa1399530:intron	30.0%
!!	TTTATTTGGGTTTTGCAGTA+AGG	-	tig0022368:70560-70579	Msa1399530:intron	30.0%
	CATTAAAAGGGTTTAATACC+TGG	+	tig0022368:70734-70753	None:intergenic	30.0%
	TTTCGTCTCAAGTACTATTT+AGG	-	tig0022368:70960-70979	Msa1399530:intron	30.0%
!	GATCTGTATCATTTGGAATA+AGG	+	tig0022368:71029-71048	None:intergenic	30.0%
	AGGATAACATTTAATGGTGT+AGG	+	tig0022368:71129-71148	None:intergenic	30.0%
!	ATTATAGCCTTTTACACCAT+TGG	-	tig0022368:71299-71318	Msa1399530:intron	30.0%
	AAAACAACCAATGGTGTAAA+AGG	+	tig0022368:71309-71328	None:intergenic	30.0%
!!	TTTTCAACCTGATTTGTCTT+GGG	-	tig0022368:71325-71344	Msa1399530:intron	30.0%
	ACATACAAGCAAAGAGAAAA+AGG	+	tig0022368:71388-71407	None:intergenic	30.0%
	TGATAGCATATTTCCTCATT+GGG	+	tig0022368:71566-71585	None:intergenic	30.0%
!	TTGATAGCATATTTCCTCAT+TGG	+	tig0022368:71567-71586	None:intergenic	30.0%
	AATGAGGAAATATGCTATCA+AGG	-	tig0022368:71566-71585	Msa1399530:intron	30.0%
	AAATATGCTATCAAGGCATA+GGG	-	tig0022368:71573-71592	Msa1399530:intron	30.0%
	CAAAACTTGTCAACTATTAG+AGG	+	tig0022368:71636-71655	None:intergenic	30.0%
!	AATTTGCTTGGGGTATAATA+TGG	-	tig0022368:71831-71850	Msa1399530:intron	30.0%
!	ACATTTAAAACCGCTAACAA+AGG	-	tig0022368:71894-71913	Msa1399530:five_prime_UTR	30.0%
	GCAGCTTACTACAAAATAAA+TGG	+	tig0022368:69966-69985	None:intergenic	30.0%
!!	TTATTTTGTAGTAAGCTGCT+AGG	-	tig0022368:69967-69986	Msa1399530:three_prime_UTR	30.0%
!	TATTTTGTAGTAAGCTGCTA+GGG	-	tig0022368:69968-69987	Msa1399530:three_prime_UTR	30.0%
!	TTTGATTTCAGAAATTAGGG+TGG	+	tig0022368:70064-70083	None:intergenic	30.0%
!!	GCACGATTGTTCTAAAAATA+AGG	+	tig0022368:70348-70367	None:intergenic	30.0%
	TATCCTTCAGAATTGGTAAA+TGG	+	tig0022368:70421-70440	None:intergenic	30.0%
!	GAAGGATAAATTGTTCCTTT+TGG	-	tig0022368:70433-70452	Msa1399530:intron	30.0%
!!	AATTGTTCCTTTTGGTGTAA+TGG	-	tig0022368:70441-70460	Msa1399530:intron	30.0%
!!!	GTTTTTGTTTTGTGACTGTA+AGG	-	tig0022368:70493-70512	Msa1399530:intron	30.0%
!!	TTTATTTGGGTTTTGCAGTA+AGG	-	tig0022368:70560-70579	Msa1399530:intron	30.0%
	CATTAAAAGGGTTTAATACC+TGG	+	tig0022368:70734-70753	None:intergenic	30.0%
	TTTCGTCTCAAGTACTATTT+AGG	-	tig0022368:70960-70979	Msa1399530:intron	30.0%
!	GATCTGTATCATTTGGAATA+AGG	+	tig0022368:71029-71048	None:intergenic	30.0%
	AGGATAACATTTAATGGTGT+AGG	+	tig0022368:71129-71148	None:intergenic	30.0%
!	ATTATAGCCTTTTACACCAT+TGG	-	tig0022368:71299-71318	Msa1399530:intron	30.0%
	AAAACAACCAATGGTGTAAA+AGG	+	tig0022368:71309-71328	None:intergenic	30.0%
!!	TTTTCAACCTGATTTGTCTT+GGG	-	tig0022368:71325-71344	Msa1399530:intron	30.0%
	ACATACAAGCAAAGAGAAAA+AGG	+	tig0022368:71388-71407	None:intergenic	30.0%
	TGATAGCATATTTCCTCATT+GGG	+	tig0022368:71566-71585	None:intergenic	30.0%
!	TTGATAGCATATTTCCTCAT+TGG	+	tig0022368:71567-71586	None:intergenic	30.0%
	AATGAGGAAATATGCTATCA+AGG	-	tig0022368:71566-71585	Msa1399530:intron	30.0%
	AAATATGCTATCAAGGCATA+GGG	-	tig0022368:71573-71592	Msa1399530:intron	30.0%
	CAAAACTTGTCAACTATTAG+AGG	+	tig0022368:71636-71655	None:intergenic	30.0%
!	AATTTGCTTGGGGTATAATA+TGG	-	tig0022368:71831-71850	Msa1399530:intron	30.0%
!	ACATTTAAAACCGCTAACAA+AGG	-	tig0022368:71894-71913	Msa1399530:five_prime_UTR	30.0%
	CTGCAGAAGAATTCAACAAT+GGG	-	tig0022368:70024-70043	Msa1399530:three_prime_UTR	35.0%
	TCAGAAATTAGGGTGGTTTA+GGG	+	tig0022368:70057-70076	None:intergenic	35.0%
	TTCAGAAATTAGGGTGGTTT+AGG	+	tig0022368:70058-70077	None:intergenic	35.0%
!!!	TGTGTTTTGATTTGCAGTGA+AGG	-	tig0022368:70128-70147	Msa1399530:three_prime_UTR	35.0%
	GAAAAACAAAGCTGATCTGT+TGG	-	tig0022368:70167-70186	Msa1399530:three_prime_UTR	35.0%
!!	TACATTTTGGAGAGCTTGTT+GGG	+	tig0022368:70258-70277	None:intergenic	35.0%
!!	TTACATTTTGGAGAGCTTGT+TGG	+	tig0022368:70259-70278	None:intergenic	35.0%
!!	AATTGGTGCTATGATCGTAA+AGG	-	tig0022368:70309-70328	Msa1399530:CDS	35.0%
	ATCAAAGCCATTACACCAAA+AGG	+	tig0022368:70451-70470	None:intergenic	35.0%
!!!	TTTGTTTTGTGACTGTAAGG+TGG	-	tig0022368:70496-70515	Msa1399530:intron	35.0%
	GCAAAAACCACAGATACATT+GGG	+	tig0022368:70799-70818	None:intergenic	35.0%
	AGCAAAAACCACAGATACAT+TGG	+	tig0022368:70800-70819	None:intergenic	35.0%
!	ACTGCATTGATGCATTTCAT+TGG	-	tig0022368:70824-70843	Msa1399530:intron	35.0%
	TATTGTGACCATCCATCAAT+TGG	-	tig0022368:70878-70897	Msa1399530:intron	35.0%
	ATACACCAATCACCAATTGA+TGG	+	tig0022368:70893-70912	None:intergenic	35.0%
!!	ATCAGGTTGAAAACAACCAA+TGG	+	tig0022368:71318-71337	None:intergenic	35.0%
!!	GTTTTCAACCTGATTTGTCT+TGG	-	tig0022368:71324-71343	Msa1399530:intron	35.0%
	TGAATATCCCAAGACAAATC+AGG	+	tig0022368:71335-71354	None:intergenic	35.0%
	GCAAAGAGAAAAAGGTTACA+AGG	+	tig0022368:71380-71399	None:intergenic	35.0%
	TTCAAGCAGAACAGTATGTT+TGG	-	tig0022368:71460-71479	Msa1399530:intron	35.0%
	GAAATATGCTATCAAGGCAT+AGG	-	tig0022368:71572-71591	Msa1399530:intron	35.0%
!	AGGGCATCATATCTATCTAT+TGG	-	tig0022368:71600-71619	Msa1399530:intron	35.0%
!	GTTTTTCTTTATCAGACTCG+AGG	-	tig0022368:71686-71705	Msa1399530:CDS	35.0%
	CAAAAAATCCTAGTAGCTTC+AGG	+	tig0022368:71719-71738	None:intergenic	35.0%
	CTGCAGAAGAATTCAACAAT+GGG	-	tig0022368:70024-70043	Msa1399530:three_prime_UTR	35.0%
	TCAGAAATTAGGGTGGTTTA+GGG	+	tig0022368:70057-70076	None:intergenic	35.0%
	TTCAGAAATTAGGGTGGTTT+AGG	+	tig0022368:70058-70077	None:intergenic	35.0%
!!!	TGTGTTTTGATTTGCAGTGA+AGG	-	tig0022368:70128-70147	Msa1399530:three_prime_UTR	35.0%
	GAAAAACAAAGCTGATCTGT+TGG	-	tig0022368:70167-70186	Msa1399530:three_prime_UTR	35.0%
!!	TACATTTTGGAGAGCTTGTT+GGG	+	tig0022368:70258-70277	None:intergenic	35.0%
!!	TTACATTTTGGAGAGCTTGT+TGG	+	tig0022368:70259-70278	None:intergenic	35.0%
!!	AATTGGTGCTATGATCGTAA+AGG	-	tig0022368:70309-70328	Msa1399530:CDS	35.0%
	ATCAAAGCCATTACACCAAA+AGG	+	tig0022368:70451-70470	None:intergenic	35.0%
!!!	TTTGTTTTGTGACTGTAAGG+TGG	-	tig0022368:70496-70515	Msa1399530:intron	35.0%
	GCAAAAACCACAGATACATT+GGG	+	tig0022368:70799-70818	None:intergenic	35.0%
	AGCAAAAACCACAGATACAT+TGG	+	tig0022368:70800-70819	None:intergenic	35.0%
!	ACTGCATTGATGCATTTCAT+TGG	-	tig0022368:70824-70843	Msa1399530:intron	35.0%
	TATTGTGACCATCCATCAAT+TGG	-	tig0022368:70878-70897	Msa1399530:intron	35.0%
	ATACACCAATCACCAATTGA+TGG	+	tig0022368:70893-70912	None:intergenic	35.0%
!!	ATCAGGTTGAAAACAACCAA+TGG	+	tig0022368:71318-71337	None:intergenic	35.0%
!!	GTTTTCAACCTGATTTGTCT+TGG	-	tig0022368:71324-71343	Msa1399530:intron	35.0%
	TGAATATCCCAAGACAAATC+AGG	+	tig0022368:71335-71354	None:intergenic	35.0%
	GCAAAGAGAAAAAGGTTACA+AGG	+	tig0022368:71380-71399	None:intergenic	35.0%
	TTCAAGCAGAACAGTATGTT+TGG	-	tig0022368:71460-71479	Msa1399530:intron	35.0%
	GAAATATGCTATCAAGGCAT+AGG	-	tig0022368:71572-71591	Msa1399530:intron	35.0%
!	AGGGCATCATATCTATCTAT+TGG	-	tig0022368:71600-71619	Msa1399530:intron	35.0%
!	GTTTTTCTTTATCAGACTCG+AGG	-	tig0022368:71686-71705	Msa1399530:CDS	35.0%
	CAAAAAATCCTAGTAGCTTC+AGG	+	tig0022368:71719-71738	None:intergenic	35.0%
	GCTGCAGAAGAATTCAACAA+TGG	-	tig0022368:70023-70042	Msa1399530:three_prime_UTR	40.0%
	GTAAAGCAAGTTCAGCTTTG+AGG	+	tig0022368:70206-70225	None:intergenic	40.0%
	CGTCCATTTACCAATTCTGA+AGG	-	tig0022368:70415-70434	Msa1399530:intron	40.0%
!!	ATTTGGGTTTTGCAGTAAGG+TGG	-	tig0022368:70563-70582	Msa1399530:intron	40.0%
	AAAACCACAGATACATTGGG+AGG	+	tig0022368:70796-70815	None:intergenic	40.0%
!	ACCATCCATCAATTGGTGAT+TGG	-	tig0022368:70885-70904	Msa1399530:intron	40.0%
	ACCAATCACCAATTGATGGA+TGG	+	tig0022368:70889-70908	None:intergenic	40.0%
	ATGAAATTCACTACACGACC+AGG	-	tig0022368:71214-71233	Msa1399530:CDS	40.0%
	CAGAAAATTGTACAGCAACC+TGG	+	tig0022368:71235-71254	None:intergenic	40.0%
	AGGAGACATATTTCCCAATG+AGG	-	tig0022368:71550-71569	Msa1399530:intron	40.0%
	TATCAAGGCATAGGGTTGTA+GGG	-	tig0022368:71581-71600	Msa1399530:intron	40.0%
	AAATCCTAGTAGCTTCAGGA+AGG	+	tig0022368:71715-71734	None:intergenic	40.0%
	ATATGGTCGATCTCACTTGA+AGG	-	tig0022368:71848-71867	Msa1399530:five_prime_UTR	40.0%
	TATGGTCGATCTCACTTGAA+GGG	-	tig0022368:71849-71868	Msa1399530:five_prime_UTR	40.0%
	GCTGCAGAAGAATTCAACAA+TGG	-	tig0022368:70023-70042	Msa1399530:three_prime_UTR	40.0%
	GTAAAGCAAGTTCAGCTTTG+AGG	+	tig0022368:70206-70225	None:intergenic	40.0%
	CGTCCATTTACCAATTCTGA+AGG	-	tig0022368:70415-70434	Msa1399530:intron	40.0%
!!	ATTTGGGTTTTGCAGTAAGG+TGG	-	tig0022368:70563-70582	Msa1399530:intron	40.0%
	AAAACCACAGATACATTGGG+AGG	+	tig0022368:70796-70815	None:intergenic	40.0%
!	ACCATCCATCAATTGGTGAT+TGG	-	tig0022368:70885-70904	Msa1399530:intron	40.0%
	ACCAATCACCAATTGATGGA+TGG	+	tig0022368:70889-70908	None:intergenic	40.0%
	ATGAAATTCACTACACGACC+AGG	-	tig0022368:71214-71233	Msa1399530:CDS	40.0%
	CAGAAAATTGTACAGCAACC+TGG	+	tig0022368:71235-71254	None:intergenic	40.0%
	AGGAGACATATTTCCCAATG+AGG	-	tig0022368:71550-71569	Msa1399530:intron	40.0%
	TATCAAGGCATAGGGTTGTA+GGG	-	tig0022368:71581-71600	Msa1399530:intron	40.0%
	AAATCCTAGTAGCTTCAGGA+AGG	+	tig0022368:71715-71734	None:intergenic	40.0%
	ATATGGTCGATCTCACTTGA+AGG	-	tig0022368:71848-71867	Msa1399530:five_prime_UTR	40.0%
	TATGGTCGATCTCACTTGAA+GGG	-	tig0022368:71849-71868	Msa1399530:five_prime_UTR	40.0%
!	TGCTTTACTCCGTGTTGCTA+AGG	-	tig0022368:70218-70237	Msa1399530:three_prime_UTR	45.0%
	CAAAGCAGCATTCTGCTTCT+GGG	+	tig0022368:70625-70644	None:intergenic	45.0%
	TCAAAGCAGCATTCTGCTTC+TGG	+	tig0022368:70626-70645	None:intergenic	45.0%
!!	AGAAGCAGAATGCTGCTTTG+AGG	-	tig0022368:70625-70644	Msa1399530:intron	45.0%
!!	GAAGCAGAATGCTGCTTTGA+GGG	-	tig0022368:70626-70645	Msa1399530:intron	45.0%
	AGGGTTTAATACCTGGTGCT+TGG	+	tig0022368:70727-70746	None:intergenic	45.0%
	TACACCTCCCAATGTATCTG+TGG	-	tig0022368:70789-70808	Msa1399530:intron	45.0%
	CCTGCAGGATCTGTATCATT+TGG	+	tig0022368:71036-71055	None:intergenic	45.0%
	CCAAATGATACAGATCCTGC+AGG	-	tig0022368:71033-71052	Msa1399530:intron	45.0%
	ATACAGATCCTGCAGGTGTA+GGG	-	tig0022368:71040-71059	Msa1399530:intron	45.0%
!	CTCCTTTTTCTTCTCCCTCT+CGG	+	tig0022368:71535-71554	None:intergenic	45.0%
	CTATCAAGGCATAGGGTTGT+AGG	-	tig0022368:71580-71599	Msa1399530:intron	45.0%
	GTTTCCTTCCTGAAGCTACT+AGG	-	tig0022368:71708-71727	Msa1399530:CDS	45.0%
	TCGATCTCACTTGAAGGGAA+GGG	-	tig0022368:71854-71873	Msa1399530:five_prime_UTR	45.0%
!	TGCTTTACTCCGTGTTGCTA+AGG	-	tig0022368:70218-70237	Msa1399530:three_prime_UTR	45.0%
	CAAAGCAGCATTCTGCTTCT+GGG	+	tig0022368:70625-70644	None:intergenic	45.0%
	TCAAAGCAGCATTCTGCTTC+TGG	+	tig0022368:70626-70645	None:intergenic	45.0%
!!	AGAAGCAGAATGCTGCTTTG+AGG	-	tig0022368:70625-70644	Msa1399530:intron	45.0%
!!	GAAGCAGAATGCTGCTTTGA+GGG	-	tig0022368:70626-70645	Msa1399530:intron	45.0%
	AGGGTTTAATACCTGGTGCT+TGG	+	tig0022368:70727-70746	None:intergenic	45.0%
	TACACCTCCCAATGTATCTG+TGG	-	tig0022368:70789-70808	Msa1399530:intron	45.0%
	CCTGCAGGATCTGTATCATT+TGG	+	tig0022368:71036-71055	None:intergenic	45.0%
	CCAAATGATACAGATCCTGC+AGG	-	tig0022368:71033-71052	Msa1399530:intron	45.0%
	ATACAGATCCTGCAGGTGTA+GGG	-	tig0022368:71040-71059	Msa1399530:intron	45.0%
!	CTCCTTTTTCTTCTCCCTCT+CGG	+	tig0022368:71535-71554	None:intergenic	45.0%
	CTATCAAGGCATAGGGTTGT+AGG	-	tig0022368:71580-71599	Msa1399530:intron	45.0%
	GTTTCCTTCCTGAAGCTACT+AGG	-	tig0022368:71708-71727	Msa1399530:CDS	45.0%
	TCGATCTCACTTGAAGGGAA+GGG	-	tig0022368:71854-71873	Msa1399530:five_prime_UTR	45.0%
	TACACACACACACACGCAAG+AGG	-	tig0022368:69993-70012	Msa1399530:three_prime_UTR	50.0%
	CAGTGAAGGTTCACGAGTTG+AGG	-	tig0022368:70142-70161	Msa1399530:three_prime_UTR	50.0%
	CACCAGTGACCTTAGCAACA+CGG	+	tig0022368:70230-70249	None:intergenic	50.0%
!	GGTTTTGCAGTAAGGTGGTG+AGG	-	tig0022368:70568-70587	Msa1399530:intron	50.0%
	TTCTTTGGGCGGAGATCAAG+TGG	+	tig0022368:70678-70697	None:intergenic	50.0%
!	GAATGGCCCTGGTCTTCTTT+GGG	+	tig0022368:70692-70711	None:intergenic	50.0%
	GATACAGATCCTGCAGGTGT+AGG	-	tig0022368:71039-71058	Msa1399530:intron	50.0%
	GACCGAGAGGGAGAAGAAAA+AGG	-	tig0022368:71530-71549	Msa1399530:intron	50.0%
	GTCGATCTCACTTGAAGGGA+AGG	-	tig0022368:71853-71872	Msa1399530:five_prime_UTR	50.0%
	TACACACACACACACGCAAG+AGG	-	tig0022368:69993-70012	Msa1399530:three_prime_UTR	50.0%
	CAGTGAAGGTTCACGAGTTG+AGG	-	tig0022368:70142-70161	Msa1399530:three_prime_UTR	50.0%
	CACCAGTGACCTTAGCAACA+CGG	+	tig0022368:70230-70249	None:intergenic	50.0%
!	GGTTTTGCAGTAAGGTGGTG+AGG	-	tig0022368:70568-70587	Msa1399530:intron	50.0%
	TTCTTTGGGCGGAGATCAAG+TGG	+	tig0022368:70678-70697	None:intergenic	50.0%
!	GAATGGCCCTGGTCTTCTTT+GGG	+	tig0022368:70692-70711	None:intergenic	50.0%
	GATACAGATCCTGCAGGTGT+AGG	-	tig0022368:71039-71058	Msa1399530:intron	50.0%
	GACCGAGAGGGAGAAGAAAA+AGG	-	tig0022368:71530-71549	Msa1399530:intron	50.0%
	GTCGATCTCACTTGAAGGGA+AGG	-	tig0022368:71853-71872	Msa1399530:five_prime_UTR	50.0%
!	CTCCGTGTTGCTAAGGTCAC+TGG	-	tig0022368:70225-70244	Msa1399530:three_prime_UTR	55.0%
!	CGTGTTGCTAAGGTCACTGG+TGG	-	tig0022368:70228-70247	Msa1399530:three_prime_UTR	55.0%
!	GGTGAGGTTGTCGATTGCTC+AGG	-	tig0022368:70584-70603	Msa1399530:intron	55.0%
	ATCTCCGCCCAAAGAAGACC+AGG	-	tig0022368:70682-70701	Msa1399530:intron	55.0%
	TCTCCGCCCAAAGAAGACCA+GGG	-	tig0022368:70683-70702	Msa1399530:intron	55.0%
!	CGAATGGCCCTGGTCTTCTT+TGG	+	tig0022368:70693-70712	None:intergenic	55.0%
	CTTGGTGAGCCTTCTGCGAA+TGG	+	tig0022368:70709-70728	None:intergenic	55.0%
	ATCCTGCAGGTGTAGGGAGA+AGG	-	tig0022368:71046-71065	Msa1399530:intron	55.0%
	ATCCTTCTCCCTACACCTGC+AGG	+	tig0022368:71051-71070	None:intergenic	55.0%
	AGTCTTCGCTGAAGACCGAG+AGG	-	tig0022368:71517-71536	Msa1399530:intron	55.0%
	GTCTTCGCTGAAGACCGAGA+GGG	-	tig0022368:71518-71537	Msa1399530:intron	55.0%
!	CTCCGTGTTGCTAAGGTCAC+TGG	-	tig0022368:70225-70244	Msa1399530:three_prime_UTR	55.0%
!	CGTGTTGCTAAGGTCACTGG+TGG	-	tig0022368:70228-70247	Msa1399530:three_prime_UTR	55.0%
!	GGTGAGGTTGTCGATTGCTC+AGG	-	tig0022368:70584-70603	Msa1399530:intron	55.0%
	ATCTCCGCCCAAAGAAGACC+AGG	-	tig0022368:70682-70701	Msa1399530:intron	55.0%
	TCTCCGCCCAAAGAAGACCA+GGG	-	tig0022368:70683-70702	Msa1399530:intron	55.0%
!	CGAATGGCCCTGGTCTTCTT+TGG	+	tig0022368:70693-70712	None:intergenic	55.0%
	CTTGGTGAGCCTTCTGCGAA+TGG	+	tig0022368:70709-70728	None:intergenic	55.0%
	ATCCTGCAGGTGTAGGGAGA+AGG	-	tig0022368:71046-71065	Msa1399530:intron	55.0%
	ATCCTTCTCCCTACACCTGC+AGG	+	tig0022368:71051-71070	None:intergenic	55.0%
	AGTCTTCGCTGAAGACCGAG+AGG	-	tig0022368:71517-71536	Msa1399530:intron	55.0%
	GTCTTCGCTGAAGACCGAGA+GGG	-	tig0022368:71518-71537	Msa1399530:intron	55.0%
!	TGGCCCTGGTCTTCTTTGGG+CGG	+	tig0022368:70689-70708	None:intergenic	60.0%
	AGACCAGGGCCATTCGCAGA+AGG	-	tig0022368:70697-70716	Msa1399530:intron	60.0%
	CAGAAGGCTCACCAAGCACC+AGG	-	tig0022368:70713-70732	Msa1399530:intron	60.0%
!	TGGCCCTGGTCTTCTTTGGG+CGG	+	tig0022368:70689-70708	None:intergenic	60.0%
	AGACCAGGGCCATTCGCAGA+AGG	-	tig0022368:70697-70716	Msa1399530:intron	60.0%
	CAGAAGGCTCACCAAGCACC+AGG	-	tig0022368:70713-70732	Msa1399530:intron	60.0%
	GAGCCTTCTGCGAATGGCCC+TGG	+	tig0022368:70703-70722	None:intergenic	65.0%
	GAGCCTTCTGCGAATGGCCC+TGG	+	tig0022368:70703-70722	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
tig0022368	gene	69965	71920	69965	ID=Msa1399530;Name=Msa1399530
tig0022368	mRNA	69965	71920	69965	ID=Msa1399530-mRNA-1;Parent=Msa1399530;Name=Msa1399530-mRNA-1;_AED=0.01;_eAED=0.01;_QI=77\|1\|1\|1\|1\|1\|4\|327\|121
tig0022368	exon	69965	70366	69965	ID=Msa1399530-mRNA-1:exon:17210;Parent=Msa1399530-mRNA-1
tig0022368	exon	71151	71307	71151	ID=Msa1399530-mRNA-1:exon:17209;Parent=Msa1399530-mRNA-1
tig0022368	exon	71611	71740	71611	ID=Msa1399530-mRNA-1:exon:17208;Parent=Msa1399530-mRNA-1
tig0022368	exon	71840	71920	71840	ID=Msa1399530-mRNA-1:exon:17207;Parent=Msa1399530-mRNA-1
tig0022368	five_prime_UTR	71844	71920	71844	ID=Msa1399530-mRNA-1:five_prime_utr;Parent=Msa1399530-mRNA-1
tig0022368	CDS	71840	71843	71840	ID=Msa1399530-mRNA-1:cds;Parent=Msa1399530-mRNA-1
tig0022368	CDS	71611	71740	71611	ID=Msa1399530-mRNA-1:cds;Parent=Msa1399530-mRNA-1
tig0022368	CDS	71151	71307	71151	ID=Msa1399530-mRNA-1:cds;Parent=Msa1399530-mRNA-1
tig0022368	CDS	70292	70366	70292	ID=Msa1399530-mRNA-1:cds;Parent=Msa1399530-mRNA-1
tig0022368	three_prime_UTR	69965	70291	69965	ID=Msa1399530-mRNA-1:three_prime_utr;Parent=Msa1399530-mRNA-1

Gene Sequence	>Msa1399530 ATGGTGAAGGTTCACGAGTTGAGGCAGAAAAACAAAGCTGATCTGTTGGCTCAGCTCAAAGACCTCAAAGCTGAACTTGCTTTACTCCGTGTTGCTAAGGTCACTGGTGGTGCACCCAACAAGCTCTCCAAAATTAAGGTGGTGAGGTTGTCGATTGCTCAGGTGTTGACAGTGATTTCCCAGAAGCAGAATGCTGCTTTGAGGGAAGTTTACAAGAACAAGAAGTATTTGCCACTTGATCTCCGCCCAAAGAAGACCAGGGCCATTCGCAGAAGGCTCACCAAGCACCAGTCTTCGCTGAAGACCGAGAGGGAGAAGAAAAAGGAGACATATTTCCCAATGAGGAAATATGCTATCAAGGCATAG
Protein sequence	>Msa1399530 MVKVHELRQKNKADLLAQLKDLKAELALLRVAKVTGGAPNKLSKIKVVRLSIAQVLTVISQKQNAALREVYKNKKYLPLDLRPKKTRAIRRRLTKHQSSLKTEREKKKETYFPMRKYAIKA*