Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0003640 | Msa0772480 | 0.801010 | 1.101643e-48 | -8.615850e-47 |
| Msa0003720 | Msa0772480 | 0.800447 | 1.435034e-48 | -8.615850e-47 |
| Msa0006040 | Msa0772480 | 0.808941 | 2.418306e-50 | -8.615850e-47 |
| Msa0006290 | Msa0772480 | 0.814429 | 1.547682e-51 | -8.615850e-47 |
| Msa0010490 | Msa0772480 | 0.833155 | 6.273013e-56 | -8.615850e-47 |
| Msa0010500 | Msa0772480 | 0.815791 | 7.712778e-52 | -8.615850e-47 |
| Msa0010820 | Msa0772480 | 0.801829 | 7.487025e-49 | -8.615850e-47 |
| Msa0011960 | Msa0772480 | 0.867986 | 9.127184e-66 | -8.615850e-47 |
| Msa0019390 | Msa0772480 | 0.865114 | 7.467607e-65 | -8.615850e-47 |
| Msa0021220 | Msa0772480 | 0.826806 | 2.215844e-54 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0772480 | MtrunA17_Chr5g0403031 | 95.109 | 184 | 9 | 0 | 1 | 184 | 1 | 184 | 1.22e-123 | 346 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0772480 | AT4G37090.1 | 45.946 | 185 | 88 | 4 | 1 | 180 | 1 | 178 | 4.92e-33 | 116 |
| Msa0772480 | AT4G37090.2 | 45.946 | 185 | 86 | 5 | 1 | 180 | 1 | 176 | 1.81e-26 | 99.8 |
Find 44 sgRNAs with CRISPR-Local
Find 193 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACAACAGGCATTTGCAA+AGG | 0.613402 | 5_2:+46853294 | None:intergenic |
| AAGAGAAAATCCAAGGATTC+AGG | 0.267478 | 5_2:-46854590 | Msa0772480:CDS |
| AAGCATTTCAACAATGCTTT+TGG | 0.238659 | 5_2:+46854772 | None:intergenic |
| AAGGCAGTCTTTAAGCATTC+AGG | 0.411790 | 5_2:+46854432 | None:intergenic |
| AAGTAGACGTAATGCTTGGT+TGG | 0.572908 | 5_2:+46854350 | None:intergenic |
| AATTAAAGGCTACCACTTCA+AGG | 0.462256 | 5_2:-46854613 | Msa0772480:CDS |
| AGAAAGAACGTCGGCGAAAA+TGG | 0.201521 | 5_2:-46854922 | Msa0772480:CDS |
| AGAAATCCCACAGCAGTGTT+TGG | 0.406449 | 5_2:+46854797 | None:intergenic |
| AGAGAAAATCCAAGGATTCA+GGG | 0.497993 | 5_2:-46854589 | Msa0772480:intron |
| AGAGATTCGAAAGATTAAAA+AGG | 0.343332 | 5_2:-46856109 | Msa0772480:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CAAAATCAGGTTTAGTTATG+AGG | - | chr5_2:46853560-46853579 | Msa0772480:intron | 30.0% | |
| TTGCAATGAGTAAAGTAACT+AGG | - | chr5_2:46853633-46853652 | Msa0772480:intron | 30.0% | |
| TGCAATGAGTAAAGTAACTA+GGG | - | chr5_2:46853634-46853653 | Msa0772480:intron | 30.0% | |
| GATATTGATCAATCTTGATG+TGG | - | chr5_2:46853758-46853777 | Msa0772480:intron | 30.0% | |
| TTATTAATTGGTTGGCACTA+CGG | - | chr5_2:46854125-46854144 | Msa0772480:intron | 30.0% | |
| TGCAGTATAAGAGAGAATAA+AGG | + | chr5_2:46854585-46854604 | None:intergenic | 30.0% | |
| TAATATGACTTTACATGGCA+GGG | - | chr5_2:46854685-46854704 | Msa0772480:intron | 30.0% | |
| ATATTGAAGAGAAAGAACGT+CGG | - | chr5_2:46854711-46854730 | Msa0772480:intron | 30.0% | |
| CCAAAGCTACTAAAAAATCT+AGG | - | chr5_2:46854768-46854787 | Msa0772480:CDS | 30.0% | |
| TTTATTAACTGAGACTGATG+AGG | - | chr5_2:46854997-46855016 | Msa0772480:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 46853270 | 46856394 | 46853270 | ID=Msa0772480;Name=Msa0772480 |
| chr5_2 | mRNA | 46853270 | 46856394 | 46853270 | ID=Msa0772480-mRNA-1;Parent=Msa0772480;Name=Msa0772480-mRNA-1;_AED=0.22;_eAED=0.22;_QI=70|1|1|1|0.8|0.66|6|131|184 |
| chr5_2 | exon | 46853270 | 46853357 | 46853270 | ID=Msa0772480-mRNA-1:exon:14032;Parent=Msa0772480-mRNA-1 |
| chr5_2 | exon | 46854277 | 46854488 | 46854277 | ID=Msa0772480-mRNA-1:exon:14031;Parent=Msa0772480-mRNA-1 |
| chr5_2 | exon | 46854590 | 46854673 | 46854590 | ID=Msa0772480-mRNA-1:exon:14030;Parent=Msa0772480-mRNA-1 |
| chr5_2 | exon | 46854758 | 46854958 | 46854758 | ID=Msa0772480-mRNA-1:exon:14029;Parent=Msa0772480-mRNA-1 |
| chr5_2 | exon | 46856096 | 46856148 | 46856096 | ID=Msa0772480-mRNA-1:exon:14028;Parent=Msa0772480-mRNA-1 |
| chr5_2 | exon | 46856277 | 46856394 | 46856277 | ID=Msa0772480-mRNA-1:exon:14027;Parent=Msa0772480-mRNA-1 |
| chr5_2 | five_prime_UTR | 46856325 | 46856394 | 46856325 | ID=Msa0772480-mRNA-1:five_prime_utr;Parent=Msa0772480-mRNA-1 |
| chr5_2 | CDS | 46856277 | 46856324 | 46856277 | ID=Msa0772480-mRNA-1:cds;Parent=Msa0772480-mRNA-1 |
| chr5_2 | CDS | 46856096 | 46856148 | 46856096 | ID=Msa0772480-mRNA-1:cds;Parent=Msa0772480-mRNA-1 |
| chr5_2 | CDS | 46854758 | 46854958 | 46854758 | ID=Msa0772480-mRNA-1:cds;Parent=Msa0772480-mRNA-1 |
| chr5_2 | CDS | 46854590 | 46854673 | 46854590 | ID=Msa0772480-mRNA-1:cds;Parent=Msa0772480-mRNA-1 |
| chr5_2 | CDS | 46854320 | 46854488 | 46854320 | ID=Msa0772480-mRNA-1:cds;Parent=Msa0772480-mRNA-1 |
| chr5_2 | three_prime_UTR | 46854277 | 46854319 | 46854277 | ID=Msa0772480-mRNA-1:three_prime_utr;Parent=Msa0772480-mRNA-1 |
| chr5_2 | three_prime_UTR | 46853270 | 46853357 | 46853270 | ID=Msa0772480-mRNA-1:three_prime_utr;Parent=Msa0772480-mRNA-1 |
| Gene Sequence |
>Msa0772480 ATGTCAGACAACGATTCTGATGCCCCTGAGGAATTCACAGCTGAACAGGGTATTCAGCTAGACGAAGAGATTCGAAAGATTAAAAAGGAAAACAAAATCAGGGTTAATATTGAAGAGAAAGAACGTCGGCGAAAATGGGCTCAAAATTTAACACCTCGACCATCCAAAGCTACTAAAAAATCTAGGGACACATCAAACACTGAATCTGAGCAAGAACCAAACACTGAATCTCAGCAAAAACCAAACACTGCTGTGGGATTTCTTCCCAAAAGCATTGTTGAAATGCTTGCAGCTAAGGAGAAAAAAAATTTATTAACTGAGACTGATGAGGAGGAAGATAAATTAAAGGCTACCACTTCAAGGAAGAGAAAATCCAAGGATTCAGGTTCTGAACCTATTATTGTGAGCAAACCAGCTCCTCCTGAATGCTTAAAGACTGCCTTGGATTTCTTGAAAGAAAGGAAAATGTCAGTTCCAAGATCGTCTGCTGTTTTGAAGAATTCCAACCAAGCATTACGTCTACTTTCCAGTTCCGGGATCATACACCAGAAATGA |
| Protein sequence |
>Msa0772480 MSDNDSDAPEEFTAEQGIQLDEEIRKIKKENKIRVNIEEKERRRKWAQNLTPRPSKATKKSRDTSNTESEQEPNTESQQKPNTAVGFLPKSIVEMLAAKEKKNLLTETDEEEDKLKATTSRKRKSKDSGSEPIIVSKPAPPECLKTALDFLKERKMSVPRSSAVLKNSNQALRLLSSSGIIHQK* |
