AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene001031

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene001031.t1 MTR_5g006440 99.234 261 2 0 1 261 1 261 0.0 536
MS.gene001031.t1 MTR_1g103100 92.720 261 19 0 1 261 1 261 7.99e-175 482
MS.gene001031.t1 MTR_3g012040 90.805 261 24 0 1 261 1 261 1.10e-169 469
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene001031.t1 AT4G34670 85.115 262 38 1 1 261 1 262 8.50e-162 449
MS.gene001031.t1 AT3G04840 84.733 262 39 1 1 261 1 262 9.92e-162 449

Find 80 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATCTCCTTACCAATTTC+TGG 0.102887 5.4:+2203931 MS.gene001031:CDS
CAACTTCCCAAGGTCAAATT+TGG 0.131525 5.4:-2204838 None:intergenic
GATCAAGCTCCAACAATTTC+TGG 0.179039 5.4:-2205226 None:intergenic
GGTCAAATTTGGGAGCTTTC+AGG 0.220044 5.4:-2204827 None:intergenic
GATATCATACCAATCCTTCT+TGG 0.266372 5.4:-2202714 None:intergenic
TTAGGTCATTGGTGCGAAAA+TGG 0.324689 5.4:+2204066 MS.gene001031:CDS
CCACTAACATACCCAGAAAT+TGG 0.324728 5.4:-2203943 None:intergenic
AACCTTGGTTTCTCGTACTC+AGG 0.324751 5.4:+2202775 MS.gene001031:CDS
GTTGAGAGCTGAAGATGTTC+AGG 0.329572 5.4:+2203905 MS.gene001031:CDS
CCTGATGGATTTGACTTGAT+TGG 0.369999 5.4:-2204205 None:intergenic
GAGATGAGGACAACGCATTC+AGG 0.376338 5.4:+2203874 MS.gene001031:CDS
CTTTGAGAATGTTCTGCATT+GGG 0.377761 5.4:+2204138 MS.gene001031:CDS
CGCCGATCCATTTGCCAAGA+AGG 0.386423 5.4:+2202700 MS.gene001031:CDS
CACCGACAAGCTTAGGTCAT+TGG 0.388612 5.4:+2204055 MS.gene001031:CDS
ATTTGACCTTGGGAAGTTGA+TGG 0.390249 5.4:+2204842 MS.gene001031:CDS
TCAACCATTGTTTCATCTGC+AGG 0.407580 5.4:-2205196 None:intergenic
TACCAATCCTTCTTGGCAAA+TGG 0.412105 5.4:-2202707 None:intergenic
AACTTCCCAAGGTCAAATTT+GGG 0.419750 5.4:-2204837 None:intergenic
AAATCTCCTTACCAATTTCT+GGG 0.420398 5.4:+2203932 MS.gene001031:CDS
ATCAAGCTCCAACAATTTCT+GGG 0.427325 5.4:-2205225 None:intergenic
CTGATGGATTTGACTTGATT+GGG 0.436527 5.4:-2204204 None:intergenic
ACAACGCATTCAGGAAGATT+AGG 0.441352 5.4:+2203883 MS.gene001031:CDS
CAGGGATAAACTTGCGTACC+AGG 0.444164 5.4:-2204725 None:intergenic
GTTTCATTGGCTGATCTTCA+AGG 0.448595 5.4:+2203852 MS.gene001031:CDS
AAGCTCCCAAATTTGACCTT+GGG 0.449450 5.4:+2204832 MS.gene001031:CDS
CACCAAGAGGCGCTCTAACC+AGG 0.467818 5.4:+2204163 MS.gene001031:CDS
AAAGCTCCCAAATTTGACCT+TGG 0.468887 5.4:+2204831 MS.gene001031:CDS
ACTTTGAGAATGTTCTGCAT+TGG 0.470669 5.4:+2204137 MS.gene001031:CDS
GAGAATCTCAAAGGGGAAGA+AGG 0.471651 5.4:+2202572 MS.gene001031:CDS
ATCCATTTGCCAAGAAGGAT+TGG 0.479936 5.4:+2202705 MS.gene001031:CDS
CACCAATGACCTAAGCTTGT+CGG 0.488210 5.4:-2204057 None:intergenic
GGCGACCTCATGTGATTTGA+AGG 0.488763 5.4:+2204701 MS.gene001031:CDS
GAAGGAACCCCAGAAATTGT+TGG 0.488913 5.4:+2205217 MS.gene001031:CDS
TCAAATCACATGAGGTCGCC+TGG 0.493748 5.4:-2204698 None:intergenic
TCGTGTGTTTGAGGTTTCAT+TGG 0.502975 5.4:+2203839 MS.gene001031:CDS
ACTCAAACATCGTGTGTTTG+AGG 0.508854 5.4:+2203830 MS.gene001031:CDS
CCAATCAAGTCAAATCCATC+AGG 0.524361 5.4:+2204205 MS.gene001031:CDS
TTCTTCACTTGGAAGACTGA+AGG 0.526406 5.4:-2202743 None:intergenic
GCAGATGAAACAATGGTTGA+AGG 0.526419 5.4:+2205199 MS.gene001031:CDS
GACTCTCATTGAAGCTCATG+TGG 0.532944 5.4:+2204091 MS.gene001031:CDS
GACCTGGTTAGAGCGCCTCT+TGG 0.533555 5.4:-2204165 None:intergenic
TCTTCACTTGGAAGACTGAA+GGG 0.535531 5.4:-2202742 None:intergenic
GAAGAACGTTGGCAAAACCT+TGG 0.541738 5.4:+2202760 MS.gene001031:CDS
CTCTGAAGATGTTGGTACAA+AGG 0.543428 5.4:+2205165 MS.gene001031:CDS
TTGAGAGCTGAAGATGTTCA+GGG 0.547196 5.4:+2203906 MS.gene001031:CDS
ATCCTTCTTGGCAAATGGAT+CGG 0.549227 5.4:-2202702 None:intergenic
CGTAACAAGTCCTCTTGACC+TGG 0.558833 5.4:-2204181 None:intergenic
CTTCACTTGGAAGACTGAAG+GGG 0.559550 5.4:-2202741 None:intergenic
ACATATCAGATTGCTTCTGA+AGG 0.563525 5.4:+2203807 MS.gene001031:intron
ACGCAAGTTTATCCCTGAGA+TGG 0.567629 5.4:+2204731 MS.gene001031:CDS
TCAGATTCGTAGAAAGATGA+GGG 0.572813 5.4:+2204662 MS.gene001031:intron
AGAATCTCAAAGGGGAAGAA+GGG 0.576770 5.4:+2202573 MS.gene001031:CDS
ATCAGATTCGTAGAAAGATG+AGG 0.577414 5.4:+2204661 MS.gene001031:intron
ACCTTGGTTTCTCGTACTCA+GGG 0.577882 5.4:+2202776 MS.gene001031:CDS
GGAGATTACTCTGAAGATGT+TGG 0.585493 5.4:+2205157 MS.gene001031:CDS
GGTTGGAAAAGAGATTGAGA+AGG 0.589251 5.4:+2204752 MS.gene001031:CDS
AACATACCCAGAAATTGGTA+AGG 0.595805 5.4:-2203938 None:intergenic
ATTCTGCAGAGGGTAGATGC+TGG 0.596121 5.4:-2204781 None:intergenic
CTCATGTGATTTGAAGGACC+TGG 0.596391 5.4:+2204707 MS.gene001031:CDS
AAGTTTATCCCTGAGATGGT+TGG 0.596683 5.4:+2204735 MS.gene001031:CDS
GGCTGATCTTCAAGGAGATG+AGG 0.598473 5.4:+2203860 MS.gene001031:CDS
GTCTTCCAAGTGAAGAACGT+TGG 0.603314 5.4:+2202749 MS.gene001031:CDS
GGCGCTCTAACCAGGTCAAG+AGG 0.606601 5.4:+2204171 MS.gene001031:CDS
CGAATGAAAACATTCTGCAG+AGG 0.608337 5.4:-2204792 None:intergenic
TGACCTTGGGAAGTTGATGG+AGG 0.615407 5.4:+2204845 MS.gene001031:CDS
GAATGAAAACATTCTGCAGA+GGG 0.617565 5.4:-2204791 None:intergenic
GAGAGCACAATCATGGCGGT+CGG 0.625040 5.4:+2202540 None:intergenic
ATCTCAAAGGGGAAGAAGGG+TGG 0.627186 5.4:+2202576 MS.gene001031:CDS
TTCTCGTACTCAGGGTACCA+AGG 0.636103 5.4:+2202784 MS.gene001031:CDS
GAGGGAGATCATGATAAACC+AGG 0.642253 5.4:+2204680 MS.gene001031:CDS
TCAAGCTCCAACAATTTCTG+GGG 0.646345 5.4:-2205224 None:intergenic
ATACCTCCATCAACTTCCCA+AGG 0.647762 5.4:-2204848 None:intergenic
TCTGCATTGGGTTCACCAAG+AGG 0.648028 5.4:+2204150 MS.gene001031:CDS
TCTCAAAGTATAGTTATCAG+TGG 0.652644 5.4:-2204123 None:intergenic
CTGCAGAGGGTAGATGCTGG+AGG 0.656069 5.4:-2204778 None:intergenic
CAATGACCTAAGCTTGTCGG+TGG 0.676242 5.4:-2204054 None:intergenic
CAGGTCCTTCAAATCACATG+AGG 0.679807 5.4:-2204706 None:intergenic
ACCCTGAGTACGAGAAACCA+AGG 0.681818 5.4:-2202777 None:intergenic
ACGACCTGCAGATGAAACAA+TGG 0.737865 5.4:+2205192 MS.gene001031:CDS
AAAACAAGAGAATCTCAAAG+GGG 0.749921 5.4:+2202565 MS.gene001031:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTGTTGAAAAAAATTAA+TGG - chr5.4:2205006-2205025 None:intergenic 10.0%
!! AAGTTCTTTATATATTTAAC+AGG - chr5.4:2203293-2203312 None:intergenic 15.0%
!! ACTGAAAAATAACAATAATA+TGG - chr5.4:2205090-2205109 None:intergenic 15.0%
!! ATAATTATAAGGTAGAAAAT+AGG - chr5.4:2204285-2204304 None:intergenic 15.0%
!! ATTAGTGTTTAATATTGTAT+AGG + chr5.4:2203568-2203587 MS.gene001031:intron 15.0%
!! ATTAGTGTTTAATATTGTTT+AGG + chr5.4:2203683-2203702 MS.gene001031:intron 15.0%
!! CTGAAAAATAACAATAATAT+GGG - chr5.4:2205089-2205108 None:intergenic 15.0%
!!! AGTGTTAATTTATTAACTTT+TGG + chr5.4:2203715-2203734 MS.gene001031:intron 15.0%
!!! GATATAATTAAGCTATTTTT+CGG + chr5.4:2205036-2205055 MS.gene001031:intron 15.0%
!!! GTTAATTCATTGTTTATTTT+TGG + chr5.4:2205122-2205141 MS.gene001031:intron 15.0%
!! AATAGTAACTTTCATTCAAA+TGG - chr5.4:2204917-2204936 None:intergenic 20.0%
!! ATAGTAACTTTCATTCAAAT+GGG - chr5.4:2204916-2204935 None:intergenic 20.0%
!! ATTTATGAGTAAAACAACAA+GGG - chr5.4:2204517-2204536 None:intergenic 20.0%
!! GAAAGAGATATACAATTATA+AGG - chr5.4:2204404-2204423 None:intergenic 20.0%
!! TATAGCTAATTAATAGCATA+TGG + chr5.4:2204373-2204392 MS.gene001031:intron 20.0%
!! TATTTATGAGTAAAACAACA+AGG - chr5.4:2204518-2204537 None:intergenic 20.0%
!! TCGGTAAAAAAAAAAAACAA+CGG + chr5.4:2205055-2205074 MS.gene001031:intron 20.0%
! AAAAACAAGAGAATCTCAAA+GGG + chr5.4:2202564-2202583 MS.gene001031:CDS 25.0%
! AAATTACAAAAGATCACACA+AGG + chr5.4:2203439-2203458 MS.gene001031:intron 25.0%
! ACATACTTGACATTATGTAT+TGG + chr5.4:2204559-2204578 MS.gene001031:intron 25.0%
! ACCAATAAGAATAAATATGC+TGG + chr5.4:2203343-2203362 MS.gene001031:intron 25.0%
! TACGAATCTGATAAAGATAA+AGG - chr5.4:2204653-2204672 None:intergenic 25.0%
! TCCAGCATATTTATTCTTAT+TGG - chr5.4:2203347-2203366 None:intergenic 25.0%
! TCGTTTAAATTTGTGAGATT+TGG + chr5.4:2202860-2202879 MS.gene001031:intron 25.0%
! TGTTATTGTGATGAATTGTT+TGG + chr5.4:2202952-2202971 MS.gene001031:intron 25.0%
!! AGTCTAAGAAACTTTTAATG+TGG - chr5.4:2203141-2203160 None:intergenic 25.0%
!! CTTATTGGTTAAATTTAAGC+TGG - chr5.4:2203332-2203351 None:intergenic 25.0%
!! TTTTCAACACATACTTCTAT+TGG + chr5.4:2203252-2203271 MS.gene001031:intron 25.0%
!!! CATTGATTTCATGTTTTTCA+CGG + chr5.4:2203177-2203196 MS.gene001031:intron 25.0%
!!! CTATGTATGTTTTTTCTACA+GGG + chr5.4:2204020-2204039 MS.gene001031:intron 25.0%
!!! TCTAATCTCTGTTTTTTCTT+AGG + chr5.4:2204583-2204602 MS.gene001031:intron 25.0%
!!! TCTATGTATGTTTTTTCTAC+AGG + chr5.4:2204019-2204038 MS.gene001031:intron 25.0%
AAAACAAGAGAATCTCAAAG+GGG + chr5.4:2202565-2202584 MS.gene001031:CDS 30.0%
AAAATCTCCTTACCAATTTC+TGG + chr5.4:2203931-2203950 MS.gene001031:CDS 30.0%
AAATCTCCTTACCAATTTCT+GGG + chr5.4:2203932-2203951 MS.gene001031:CDS 30.0%
AGCTGGTACAGATAATTATA+AGG - chr5.4:2204296-2204315 None:intergenic 30.0%
ATGAAAGAATGAGAGTGAAA+AGG - chr5.4:2202633-2202652 None:intergenic 30.0%
ATTGTAGTGAAAATACCTGA+TGG - chr5.4:2204223-2204242 None:intergenic 30.0%
CAAAAACAAGAGAATCTCAA+AGG + chr5.4:2202563-2202582 MS.gene001031:CDS 30.0%
CCATGTCAATCAAATCAAAT+TGG - chr5.4:2203757-2203776 None:intergenic 30.0%
GAAATCAATGATATGAAGTG+AGG - chr5.4:2203167-2203186 None:intergenic 30.0%
GTCAAGTATGTATCAAAACA+AGG - chr5.4:2204550-2204569 None:intergenic 30.0%
TAACCAAAACAGCTCAATAT+GGG - chr5.4:2204975-2204994 None:intergenic 30.0%
TATGTATCAAAACAAGGAGT+AGG - chr5.4:2204544-2204563 None:intergenic 30.0%
TCACAAATTTAAACGAAGCT+CGG - chr5.4:2202856-2202875 None:intergenic 30.0%
TCTCAAAGTATAGTTATCAG+TGG - chr5.4:2204126-2204145 None:intergenic 30.0%
TGAAAGAATGAGAGTGAAAA+GGG - chr5.4:2202632-2202651 None:intergenic 30.0%
TGACTAACATTCTAGTGTAA+AGG - chr5.4:2204951-2204970 None:intergenic 30.0%
TTAACCAAAACAGCTCAATA+TGG - chr5.4:2204976-2204995 None:intergenic 30.0%
TTTCACTACAATATTGCTAG+TGG + chr5.4:2204231-2204250 MS.gene001031:intron 30.0%
! ATGAAAGAAAAAGTGTACCT+TGG - chr5.4:2202804-2202823 None:intergenic 30.0%
! TTGAGTGAATTTGTTCTTAC+TGG + chr5.4:2203621-2203640 MS.gene001031:intron 30.0%
!! CCAATTTGATTTGATTGACA+TGG + chr5.4:2203754-2203773 MS.gene001031:intron 30.0%
!! TCTCTTTGCATTTTAGCAAT+TGG + chr5.4:2203377-2203396 MS.gene001031:intron 30.0%
!!! CATTGTTTATTTTTGGTCGT+AGG + chr5.4:2205129-2205148 MS.gene001031:intron 30.0%
!!! CTCTGTTTTTTCTTAGGAAA+TGG + chr5.4:2204589-2204608 MS.gene001031:intron 30.0%
!!! GTATCAGTTGAGTCATTTTT+TGG + chr5.4:2203405-2203424 MS.gene001031:intron 30.0%
!!! TATGTTTTTTCTACAGGGAA+TGG + chr5.4:2204025-2204044 MS.gene001031:intron 30.0%
!!! TGAGTCATTTTTTGGTACAA+AGG + chr5.4:2203413-2203432 MS.gene001031:intron 30.0%
AACATACCCAGAAATTGGTA+AGG - chr5.4:2203941-2203960 None:intergenic 35.0%
AACTTCCCAAGGTCAAATTT+GGG - chr5.4:2204840-2204859 None:intergenic 35.0%
AATTATCTGTACCAGCTTGT+AGG + chr5.4:2204299-2204318 MS.gene001031:intron 35.0%
ACATATCAGATTGCTTCTGA+AGG + chr5.4:2203807-2203826 MS.gene001031:intron 35.0%
ATCAGATTCGTAGAAAGATG+AGG + chr5.4:2204661-2204680 MS.gene001031:intron 35.0%
ATCATACCCATTGGCATTAT+GGG - chr5.4:2203015-2203034 None:intergenic 35.0%
CCACAAAATATCATACCCAT+TGG - chr5.4:2203024-2203043 None:intergenic 35.0%
GAATGAAAACATTCTGCAGA+GGG - chr5.4:2204794-2204813 None:intergenic 35.0%
GATATCATACCAATCCTTCT+TGG - chr5.4:2202717-2202736 None:intergenic 35.0%
TATCATACCCATTGGCATTA+TGG - chr5.4:2203016-2203035 None:intergenic 35.0%
TATGAGTAAAACAACAAGGG+TGG - chr5.4:2204514-2204533 None:intergenic 35.0%
TCAGATTCGTAGAAAGATGA+GGG + chr5.4:2204662-2204681 MS.gene001031:intron 35.0%
TTCAAATGGGAACAAAGTAG+TGG - chr5.4:2204903-2204922 None:intergenic 35.0%
! ACTTTGAGAATGTTCTGCAT+TGG + chr5.4:2204137-2204156 MS.gene001031:CDS 35.0%
! CCAATGGGTATGATATTTTG+TGG + chr5.4:2203021-2203040 MS.gene001031:intron 35.0%
! CTTTGAGAATGTTCTGCATT+GGG + chr5.4:2204138-2204157 MS.gene001031:CDS 35.0%
!! CACAAGGTATTTTCTGTTCA+AGG + chr5.4:2203455-2203474 MS.gene001031:intron 35.0%
!! CTGATGGATTTGACTTGATT+GGG - chr5.4:2204207-2204226 None:intergenic 35.0%
!!! TATTTTTGGTCGTAGGTTCA+TGG + chr5.4:2205136-2205155 MS.gene001031:intron 35.0%
AAAGCTCCCAAATTTGACCT+TGG + chr5.4:2204831-2204850 MS.gene001031:CDS 40.0%
AAGCTCCCAAATTTGACCTT+GGG + chr5.4:2204832-2204851 MS.gene001031:CDS 40.0%
AAGTTTATCCCTGAGATGGT+TGG + chr5.4:2204735-2204754 MS.gene001031:CDS 40.0%
ACAACGCATTCAGGAAGATT+AGG + chr5.4:2203883-2203902 MS.gene001031:CDS 40.0%
ACACATAGATACCACACAGT+AGG - chr5.4:2202908-2202927 None:intergenic 40.0%
ACTCAAACATCGTGTGTTTG+AGG + chr5.4:2203830-2203849 MS.gene001031:CDS 40.0%
AGAATCTCAAAGGGGAAGAA+GGG + chr5.4:2202573-2202592 MS.gene001031:CDS 40.0%
AGCTTATCCTCATTCCATCT+AGG - chr5.4:2203535-2203554 None:intergenic 40.0%
ATCCATTTGCCAAGAAGGAT+TGG + chr5.4:2202705-2202724 MS.gene001031:CDS 40.0%
ATCCTTCTTGGCAAATGGAT+CGG - chr5.4:2202705-2202724 None:intergenic 40.0%
ATTTGACCTTGGGAAGTTGA+TGG + chr5.4:2204842-2204861 MS.gene001031:CDS 40.0%
CAACTTCCCAAGGTCAAATT+TGG - chr5.4:2204841-2204860 None:intergenic 40.0%
CCAATCAAGTCAAATCCATC+AGG + chr5.4:2204205-2204224 MS.gene001031:CDS 40.0%
CCAATTAACATCCTACAAGC+TGG - chr5.4:2204313-2204332 None:intergenic 40.0%
CCACTAACATACCCAGAAAT+TGG - chr5.4:2203946-2203965 None:intergenic 40.0%
CCAGCTTGTAGGATGTTAAT+TGG + chr5.4:2204310-2204329 MS.gene001031:intron 40.0%
CGAATGAAAACATTCTGCAG+AGG - chr5.4:2204795-2204814 None:intergenic 40.0%
GCAGATGAAACAATGGTTGA+AGG + chr5.4:2205199-2205218 MS.gene001031:CDS 40.0%
GGTTGGAAAAGAGATTGAGA+AGG + chr5.4:2204752-2204771 MS.gene001031:CDS 40.0%
TACCAATCCTTCTTGGCAAA+TGG - chr5.4:2202710-2202729 None:intergenic 40.0%
TCAACCATTGTTTCATCTGC+AGG - chr5.4:2205199-2205218 None:intergenic 40.0%
TCAAGCTCCAACAATTTCTG+GGG - chr5.4:2205227-2205246 None:intergenic 40.0%
TCTTCACTTGGAAGACTGAA+GGG - chr5.4:2202745-2202764 None:intergenic 40.0%
TGTGTGGTATCTATGTGTTG+TGG + chr5.4:2202910-2202929 MS.gene001031:intron 40.0%
TTAGGTCATTGGTGCGAAAA+TGG + chr5.4:2204066-2204085 MS.gene001031:CDS 40.0%
TTCTTCACTTGGAAGACTGA+AGG - chr5.4:2202746-2202765 None:intergenic 40.0%
TTGAGAGCTGAAGATGTTCA+GGG + chr5.4:2203906-2203925 MS.gene001031:CDS 40.0%
! AGTGGTTTCAAGTCTAGTAG+TGG - chr5.4:2204885-2204904 None:intergenic 40.0%
! ATCCTCATTCCATCTAGGAT+AGG - chr5.4:2203530-2203549 None:intergenic 40.0%
! ATCTCTTTTCCAACCATCTC+AGG - chr5.4:2204747-2204766 None:intergenic 40.0%
! ATTTTACCACCGACAAGCTT+AGG + chr5.4:2204048-2204067 MS.gene001031:CDS 40.0%
! CAACCCATATTGAGCTGTTT+TGG + chr5.4:2204969-2204988 MS.gene001031:intron 40.0%
! CCAATTTCTGGGTATGTTAG+TGG + chr5.4:2203943-2203962 MS.gene001031:intron 40.0%
! CTCTGAAGATGTTGGTACAA+AGG + chr5.4:2205165-2205184 MS.gene001031:CDS 40.0%
! GATTTCATGTTTTTCACGGC+TGG + chr5.4:2203181-2203200 MS.gene001031:intron 40.0%
! GGAGATTACTCTGAAGATGT+TGG + chr5.4:2205157-2205176 MS.gene001031:CDS 40.0%
! GTTTCATTGGCTGATCTTCA+AGG + chr5.4:2203852-2203871 MS.gene001031:CDS 40.0%
! TCCTCATTCCATCTAGGATA+GGG - chr5.4:2203529-2203548 None:intergenic 40.0%
! TCGTGTGTTTGAGGTTTCAT+TGG + chr5.4:2203839-2203858 MS.gene001031:CDS 40.0%
! TCTCTTTTCCAACCATCTCA+GGG - chr5.4:2204746-2204765 None:intergenic 40.0%
! TTTTGCCAACGTTCTTCACT+TGG - chr5.4:2202757-2202776 None:intergenic 40.0%
!! CCTGATGGATTTGACTTGAT+TGG - chr5.4:2204208-2204227 None:intergenic 40.0%
AACCTTGGTTTCTCGTACTC+AGG + chr5.4:2202775-2202794 MS.gene001031:CDS 45.0%
ACCTTGGTTTCTCGTACTCA+GGG + chr5.4:2202776-2202795 MS.gene001031:CDS 45.0%
ACGACCTGCAGATGAAACAA+TGG + chr5.4:2205192-2205211 MS.gene001031:CDS 45.0%
ACGCAAGTTTATCCCTGAGA+TGG + chr5.4:2204731-2204750 MS.gene001031:CDS 45.0%
ACTGATCTCTTCCTACTGTG+TGG + chr5.4:2202894-2202913 MS.gene001031:intron 45.0%
ACTGTGTCCCATAATGCCAA+TGG + chr5.4:2203005-2203024 MS.gene001031:intron 45.0%
ATACCTCCATCAACTTCCCA+AGG - chr5.4:2204851-2204870 None:intergenic 45.0%
CACCAATGACCTAAGCTTGT+CGG - chr5.4:2204060-2204079 None:intergenic 45.0%
CAGGTCCTTCAAATCACATG+AGG - chr5.4:2204709-2204728 None:intergenic 45.0%
CTCATGTGATTTGAAGGACC+TGG + chr5.4:2204707-2204726 MS.gene001031:CDS 45.0%
CTGTGTCCCATAATGCCAAT+GGG + chr5.4:2203006-2203025 MS.gene001031:intron 45.0%
CTTCACTTGGAAGACTGAAG+GGG - chr5.4:2202744-2202763 None:intergenic 45.0%
GAAGAACGTTGGCAAAACCT+TGG + chr5.4:2202760-2202779 MS.gene001031:CDS 45.0%
GAAGGAACCCCAGAAATTGT+TGG + chr5.4:2205217-2205236 MS.gene001031:CDS 45.0%
GACTCTCATTGAAGCTCATG+TGG + chr5.4:2204091-2204110 MS.gene001031:CDS 45.0%
GAGAATCTCAAAGGGGAAGA+AGG + chr5.4:2202572-2202591 MS.gene001031:CDS 45.0%
GAGGGAGATCATGATAAACC+AGG + chr5.4:2204680-2204699 MS.gene001031:CDS 45.0%
GGTCAAATTTGGGAGCTTTC+AGG - chr5.4:2204830-2204849 None:intergenic 45.0%
GTCTTCCAAGTGAAGAACGT+TGG + chr5.4:2202749-2202768 MS.gene001031:CDS 45.0%
GTTGAGAGCTGAAGATGTTC+AGG + chr5.4:2203905-2203924 MS.gene001031:CDS 45.0%
! AAAATGCAAAGAGAGCGCAC+TGG - chr5.4:2203371-2203390 None:intergenic 45.0%
!! AGTCAGCTTTGGGAATTCAC+AGG - chr5.4:2203492-2203511 None:intergenic 45.0%
ACCCTGAGTACGAGAAACCA+AGG - chr5.4:2202780-2202799 None:intergenic 50.0%
ACCTCTTGCCCTATCCTAGA+TGG + chr5.4:2203518-2203537 MS.gene001031:intron 50.0%
AGGTGGAGCAAGTCAGCTTT+GGG - chr5.4:2203502-2203521 None:intergenic 50.0%
ATCTCAAAGGGGAAGAAGGG+TGG + chr5.4:2202576-2202595 MS.gene001031:CDS 50.0%
ATTCTGCAGAGGGTAGATGC+TGG - chr5.4:2204784-2204803 None:intergenic 50.0%
CAATGACCTAAGCTTGTCGG+TGG - chr5.4:2204057-2204076 None:intergenic 50.0%
CACCGACAAGCTTAGGTCAT+TGG + chr5.4:2204055-2204074 MS.gene001031:CDS 50.0%
CAGGGATAAACTTGCGTACC+AGG - chr5.4:2204728-2204747 None:intergenic 50.0%
CGTAACAAGTCCTCTTGACC+TGG - chr5.4:2204184-2204203 None:intergenic 50.0%
GAGATGAGGACAACGCATTC+AGG + chr5.4:2203874-2203893 MS.gene001031:CDS 50.0%
GCCCTATCCTAGATGGAATG+AGG + chr5.4:2203525-2203544 MS.gene001031:intron 50.0%
GGCGACCTCATGTGATTTGA+AGG + chr5.4:2204701-2204720 MS.gene001031:CDS 50.0%
GGCTGATCTTCAAGGAGATG+AGG + chr5.4:2203860-2203879 MS.gene001031:CDS 50.0%
TCAAATCACATGAGGTCGCC+TGG - chr5.4:2204701-2204720 None:intergenic 50.0%
TCTGCATTGGGTTCACCAAG+AGG + chr5.4:2204150-2204169 MS.gene001031:CDS 50.0%
! ATCTAGGATAGGGCAAGAGG+TGG - chr5.4:2203519-2203538 None:intergenic 50.0%
! TCCATCTAGGATAGGGCAAG+AGG - chr5.4:2203522-2203541 None:intergenic 50.0%
! TTCTCGTACTCAGGGTACCA+AGG + chr5.4:2202784-2202803 MS.gene001031:CDS 50.0%
!! CAGCTTTGGGAATTCACAGG+TGG - chr5.4:2203489-2203508 None:intergenic 50.0%
!! TGACCTTGGGAAGTTGATGG+AGG + chr5.4:2204845-2204864 MS.gene001031:CDS 50.0%
CGCCGATCCATTTGCCAAGA+AGG + chr5.4:2202700-2202719 MS.gene001031:CDS 55.0%
GAGGTGGAGCAAGTCAGCTT+TGG - chr5.4:2203503-2203522 None:intergenic 55.0%
CTGCAGAGGGTAGATGCTGG+AGG - chr5.4:2204781-2204800 None:intergenic 60.0%
GGCGCTCTAACCAGGTCAAG+AGG + chr5.4:2204171-2204190 MS.gene001031:CDS 60.0%
! GACCTGGTTAGAGCGCCTCT+TGG - chr5.4:2204168-2204187 None:intergenic 60.0%
!! CACCAAGAGGCGCTCTAACC+AGG + chr5.4:2204163-2204182 MS.gene001031:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 2202552 2205246 2202552 ID=MS.gene001031
chr5.4 mRNA 2202552 2205246 2202552 ID=MS.gene001031.t1;Parent=MS.gene001031
chr5.4 exon 2202552 2202610 2202552 ID=MS.gene001031.t1.exon1;Parent=MS.gene001031.t1
chr5.4 CDS 2202552 2202610 2202552 ID=cds.MS.gene001031.t1;Parent=MS.gene001031.t1
chr5.4 exon 2202700 2202805 2202700 ID=MS.gene001031.t1.exon2;Parent=MS.gene001031.t1
chr5.4 CDS 2202700 2202805 2202700 ID=cds.MS.gene001031.t1;Parent=MS.gene001031.t1
chr5.4 exon 2203816 2203953 2203816 ID=MS.gene001031.t1.exon3;Parent=MS.gene001031.t1
chr5.4 CDS 2203816 2203953 2203816 ID=cds.MS.gene001031.t1;Parent=MS.gene001031.t1
chr5.4 exon 2204041 2204226 2204041 ID=MS.gene001031.t1.exon4;Parent=MS.gene001031.t1
chr5.4 CDS 2204041 2204226 2204041 ID=cds.MS.gene001031.t1;Parent=MS.gene001031.t1
chr5.4 exon 2204666 2204866 2204666 ID=MS.gene001031.t1.exon5;Parent=MS.gene001031.t1
chr5.4 CDS 2204666 2204866 2204666 ID=cds.MS.gene001031.t1;Parent=MS.gene001031.t1
chr5.4 exon 2205151 2205246 2205151 ID=MS.gene001031.t1.exon6;Parent=MS.gene001031.t1
chr5.4 CDS 2205151 2205246 2205151 ID=cds.MS.gene001031.t1;Parent=MS.gene001031.t1
Gene Sequence

>MS.gene001031

ATGGCGGTCGGCAAAAACAAGAGAATCTCAAAGGGGAAGAAGGGTGGCAAGAAGAAAGCGTAAGCTTTTCATTCATTCCCTTTTCACTCTCATTCTTTCATTTTTGTTATTTAGCTCAGATTTTTTTGATTCAATTATCAATTTACAGCGCCGATCCATTTGCCAAGAAGGATTGGTATGATATCAAAGCCCCTTCAGTCTTCCAAGTGAAGAACGTTGGCAAAACCTTGGTTTCTCGTACTCAGGGTACCAAGGTACACTTTTTCTTTCATATTCTGTATCAAAATTTATAATATTTTAACCGAGCTTCGTTTAAATTTGTGAGATTTGGTTATTTTGTTTACTGATCTCTTCCTACTGTGTGGTATCTATGTGTTGTGGAATTTAGATTGCTCTTAAATGTTATTGTGATGAATTGTTTGGCAATATAGTTTATAGAACTAATGTTGTTATACTGTGTCCCATAATGCCAATGGGTATGATATTTTGTGGTTTTTAATTTAATTTTTTTTATCTTATCTGAAGAACATTAACAATGAATATAGTCTATAAAATGAATGCTTATATGCTTCATTAATACAGATGACCACATTAAAAGTTTCTTAGACTAGACCTCACTTCATATCATTGATTTCATGTTTTTCACGGCTGGAAATAAAGAATGAAAATTGCTAGCAACATACTACTCACTATTACAATCTTTTCAACACATACTTCTATTGGTTGAAATTTATAAATCCTGTTAAATATATAAAGAACTTGCATAATTAAGTGAGACCAGCTTAAATTTAACCAATAAGAATAAATATGCTGGAACCAGTGCGCTCTCTTTGCATTTTAGCAATTGGCAAATGTATCAGTTGAGTCATTTTTTGGTACAAAGGTAAAAATTACAAAAGATCACACAAGGTATTTTCTGTTCAAGGATGCAATTCCACCTGTGAATTCCCAAAGCTGACTTGCTCCACCTCTTGCCCTATCCTAGATGGAATGAGGATAAGCTTAGATCATGATTAATTAGTGTTTAATATTGTATAGGAGTTTTATATTATTTTTTAAACTTATAGTTTTGAGTGAATTTGTTCTTACTGGTAATGTAATTTTTTGACTTGATTTTCTAGTTCATGATTAATTAGTGTTTAATATTGTTTAGGAGTCTTAAGAGTGTTAATTTATTAACTTTTGGTGTATTTGTTGTATGTCCAATTTGATTTGATTGACATGGTTATCTTAATGATGATTCTATGTTGTTTTTACATATCAGATTGCTTCTGAAGGACTCAAACATCGTGTGTTTGAGGTTTCATTGGCTGATCTTCAAGGAGATGAGGACAACGCATTCAGGAAGATTAGGTTGAGAGCTGAAGATGTTCAGGGAAAAAATCTCCTTACCAATTTCTGGGTATGTTAGTGGCTGTGTATTTTTTTTATTTTGTTTTATTGTTGCATGAAAATTGTATGATGTATTCTATGTATGTTTTTTCTACAGGGAATGGATTTTACCACCGACAAGCTTAGGTCATTGGTGCGAAAATGGCAGACTCTCATTGAAGCTCATGTGGATGTTAAGACCACTGATAACTATACTTTGAGAATGTTCTGCATTGGGTTCACCAAGAGGCGCTCTAACCAGGTCAAGAGGACTTGTTACGCCCAATCAAGTCAAATCCATCAGGTATTTTCACTACAATATTGCTAGTGGTTTCATTTGAACTCTGCTTTGTCTTCAACCTATTTTCTACCTTATAATTATCTGTACCAGCTTGTAGGATGTTAATTGGTGTACACTGTAATTGCATATCTCTTTTAGTCAAGCACTTATATAGCTAATTAATAGCATATGGTGTGACCTTATAATTGTATATCTCTTTCAATCAAGCACATATAACTAATTTATAGTGTATGTAGTAAATTAAGTTATATCTTTACTAGTTAATTTTTCTCTCTGATTTTGCATATCCACCCTTGTTGTTTTACTCATAAATATCTCCTACTCCTTGTTTTGATACATACTTGACATTATGTATTGGTTCTAATCTCTGTTTTTTCTTAGGAAATGGTTTGTTATGTCAGATAGCATAAAAAGCTTATATGTTTTCCTTTATCTTTATCAGATTCGTAGAAAGATGAGGGAGATCATGATAAACCAGGCGACCTCATGTGATTTGAAGGACCTGGTACGCAAGTTTATCCCTGAGATGGTTGGAAAAGAGATTGAGAAGGCTACCTCCAGCATCTACCCTCTGCAGAATGTTTTCATTCGTAAAGTTAAGATCCTGAAAGCTCCCAAATTTGACCTTGGGAAGTTGATGGAGGTATGTCTTTTATGTCCACTACTAGACTTGAAACCACTACTTTGTTCCCATTTGAATGAAAGTTACTATTATTTAATGATTCCTTTACACTAGAATGTTAGTCAACCCATATTGAGCTGTTTTGGTTAAAGATTTTCCATTAATTTTTTTCAACAAATTATGATCGTAAGATATAATTAAGCTATTTTTCGGTAAAAAAAAAAAACAACGGTTGAATGACCCATATTATTGTTATTTTTCAGTTTTCGTCAGTTTGTTAATTCATTGTTTATTTTTGGTCGTAGGTTCATGGAGATTACTCTGAAGATGTTGGTACAAAGGTTGAACGACCTGCAGATGAAACAATGGTTGAAGGAACCCCAGAAATTGTTGGAGCTTGA
Protein sequence

>MS.gene001031.t1

MAVGKNKRISKGKKGGKKKAADPFAKKDWYDIKAPSVFQVKNVGKTLVSRTQGTKIASEGLKHRVFEVSLADLQGDEDNAFRKIRLRAEDVQGKNLLTNFWGMDFTTDKLRSLVRKWQTLIEAHVDVKTTDNYTLRMFCIGFTKRRSNQVKRTCYAQSSQIHQIRRKMREIMINQATSCDLKDLVRKFIPEMVGKEIEKATSSIYPLQNVFIRKVKILKAPKFDLGKLMEVHGDYSEDVGTKVERPADETMVEGTPEIVGA