Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024860.t1 | XP_003610736.1 | 99.6 | 261 | 1 | 0 | 1 | 261 | 1 | 261 | 3.50E-142 | 514.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024860.t1 | Q8GTE3 | 96.2 | 261 | 10 | 0 | 1 | 261 | 1 | 261 | 7.3e-141 | 501.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024860.t1 | G7K3P1 | 99.6 | 261 | 1 | 0 | 1 | 261 | 1 | 261 | 2.5e-142 | 514.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024860.t1 | MTR_5g006440 | 99.617 | 261 | 1 | 0 | 1 | 261 | 1 | 261 | 0.0 | 538 |
| MS.gene024860.t1 | MTR_1g103100 | 93.103 | 261 | 18 | 0 | 1 | 261 | 1 | 261 | 7.98e-176 | 484 |
| MS.gene024860.t1 | MTR_3g012040 | 91.188 | 261 | 23 | 0 | 1 | 261 | 1 | 261 | 1.05e-170 | 471 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024860.t1 | AT4G34670 | 85.496 | 262 | 37 | 1 | 1 | 261 | 1 | 262 | 1.35e-162 | 451 |
| MS.gene024860.t1 | AT3G04840 | 85.115 | 262 | 38 | 1 | 1 | 261 | 1 | 262 | 1.44e-162 | 451 |
Find 80 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATCTCCTTACCAATTTC+TGG | 0.102887 | 5.3:+1569806 | MS.gene024860:CDS |
| CAACTTCCCAAGGTCAAATT+TGG | 0.131525 | 5.3:-1570713 | None:intergenic |
| GATCAAGCTCCAACAATTTC+TGG | 0.179039 | 5.3:-1571254 | None:intergenic |
| GGTCAAATTTGGGAGCTTTC+AGG | 0.220044 | 5.3:-1570702 | None:intergenic |
| GATATCATACCAATCCTTCT+TGG | 0.266372 | 5.3:-1556873 | None:intergenic |
| TTAGGTCATTGGTGCGAAAA+TGG | 0.324689 | 5.3:+1569941 | MS.gene024860:CDS |
| CCACTAACATACCCAGAAAT+TGG | 0.324728 | 5.3:-1569818 | None:intergenic |
| AACCTTGGTTTCTCGTACTC+AGG | 0.324751 | 5.3:+1556934 | MS.gene024860:CDS |
| GTTGAGAGCTGAAGATGTTC+AGG | 0.329572 | 5.3:+1569780 | MS.gene024860:CDS |
| CCTGACGGATTTGACTTGAT+TGG | 0.374179 | 5.3:-1570080 | None:intergenic |
| GAGATGAGGACAACGCATTC+AGG | 0.376338 | 5.3:+1569749 | MS.gene024860:CDS |
| CTTTGAGAATGTTCTGCATT+GGG | 0.377761 | 5.3:+1570013 | MS.gene024860:CDS |
| CGCCGATCCATTTGCCAAGA+AGG | 0.386423 | 5.3:+1556859 | MS.gene024860:CDS |
| CACCGACAAGCTTAGGTCAT+TGG | 0.388612 | 5.3:+1569930 | MS.gene024860:CDS |
| ATTTGACCTTGGGAAGTTGA+TGG | 0.390249 | 5.3:+1570717 | MS.gene024860:CDS |
| CTGACGGATTTGACTTGATT+GGG | 0.398861 | 5.3:-1570079 | None:intergenic |
| TCAACCATTGTTTCATCTGC+AGG | 0.407580 | 5.3:-1571224 | None:intergenic |
| TACCAATCCTTCTTGGCAAA+TGG | 0.412105 | 5.3:-1556866 | None:intergenic |
| AACTTCCCAAGGTCAAATTT+GGG | 0.419750 | 5.3:-1570712 | None:intergenic |
| AAATCTCCTTACCAATTTCT+GGG | 0.420398 | 5.3:+1569807 | MS.gene024860:CDS |
| ATCAAGCTCCAACAATTTCT+GGG | 0.427325 | 5.3:-1571253 | None:intergenic |
| ACAACGCATTCAGGAAGATT+AGG | 0.441352 | 5.3:+1569758 | MS.gene024860:CDS |
| CAGGGATAAACTTGCGTACC+AGG | 0.444164 | 5.3:-1570600 | None:intergenic |
| GTTTCATTGGCTGATCTTCA+AGG | 0.448595 | 5.3:+1569727 | MS.gene024860:CDS |
| AAGCTCCCAAATTTGACCTT+GGG | 0.449450 | 5.3:+1570707 | MS.gene024860:CDS |
| CACCAAGAGGCGCTCTAACC+AGG | 0.467818 | 5.3:+1570038 | MS.gene024860:CDS |
| AAAGCTCCCAAATTTGACCT+TGG | 0.468887 | 5.3:+1570706 | MS.gene024860:CDS |
| ACTTTGAGAATGTTCTGCAT+TGG | 0.470669 | 5.3:+1570012 | MS.gene024860:CDS |
| GAGAATCTCAAAGGGGAAGA+AGG | 0.471651 | 5.3:+1556731 | MS.gene024860:CDS |
| ATCCATTTGCCAAGAAGGAT+TGG | 0.479936 | 5.3:+1556864 | MS.gene024860:CDS |
| CACCAATGACCTAAGCTTGT+CGG | 0.488210 | 5.3:-1569932 | None:intergenic |
| GGCGACCTCATGTGATTTGA+AGG | 0.488763 | 5.3:+1570576 | MS.gene024860:CDS |
| GAAGGAACCCCAGAAATTGT+TGG | 0.488913 | 5.3:+1571245 | MS.gene024860:CDS |
| TCAAATCACATGAGGTCGCC+TGG | 0.493748 | 5.3:-1570573 | None:intergenic |
| TCGTGTGTTTGAGGTTTCAT+TGG | 0.502975 | 5.3:+1569714 | MS.gene024860:CDS |
| ACTCAAACATCGTGTGTTTG+AGG | 0.508854 | 5.3:+1569705 | MS.gene024860:CDS |
| TTCTTCACTTGGAAGACTGA+AGG | 0.526406 | 5.3:-1556902 | None:intergenic |
| GCAGATGAAACAATGGTTGA+AGG | 0.526419 | 5.3:+1571227 | MS.gene024860:CDS |
| GACTCTCATTGAAGCTCATG+TGG | 0.532944 | 5.3:+1569966 | MS.gene024860:CDS |
| GACCTGGTTAGAGCGCCTCT+TGG | 0.533555 | 5.3:-1570040 | None:intergenic |
| TCTTCACTTGGAAGACTGAA+GGG | 0.535531 | 5.3:-1556901 | None:intergenic |
| CCAATCAAGTCAAATCCGTC+AGG | 0.535673 | 5.3:+1570080 | MS.gene024860:CDS |
| GAAGAACGTTGGCAAAACCT+TGG | 0.541738 | 5.3:+1556919 | MS.gene024860:CDS |
| CTCTGAAGATGTTGGTACAA+AGG | 0.543428 | 5.3:+1571193 | MS.gene024860:CDS |
| TTGAGAGCTGAAGATGTTCA+GGG | 0.547196 | 5.3:+1569781 | MS.gene024860:CDS |
| ATCCTTCTTGGCAAATGGAT+CGG | 0.549227 | 5.3:-1556861 | None:intergenic |
| CGTAACAAGTCCTCTTGACC+TGG | 0.558833 | 5.3:-1570056 | None:intergenic |
| CTTCACTTGGAAGACTGAAG+GGG | 0.559550 | 5.3:-1556900 | None:intergenic |
| ACATATCAGATTGCTTCTGA+AGG | 0.563525 | 5.3:+1569682 | MS.gene024860:intron |
| ACGCAAGTTTATCCCTGAGA+TGG | 0.567629 | 5.3:+1570606 | MS.gene024860:CDS |
| TCAGATTCGTAGAAAGATGA+GGG | 0.572813 | 5.3:+1570537 | MS.gene024860:intron |
| AGAATCTCAAAGGGGAAGAA+GGG | 0.576770 | 5.3:+1556732 | MS.gene024860:CDS |
| ATCAGATTCGTAGAAAGATG+AGG | 0.577414 | 5.3:+1570536 | MS.gene024860:intron |
| ACCTTGGTTTCTCGTACTCA+GGG | 0.577882 | 5.3:+1556935 | MS.gene024860:CDS |
| GGAGATTACTCTGAAGATGT+TGG | 0.585493 | 5.3:+1571185 | MS.gene024860:CDS |
| GGTTGGAAAAGAGATTGAGA+AGG | 0.589251 | 5.3:+1570627 | MS.gene024860:CDS |
| AACATACCCAGAAATTGGTA+AGG | 0.595805 | 5.3:-1569813 | None:intergenic |
| ATTCTGCAGAGGGTAGATGC+TGG | 0.596121 | 5.3:-1570656 | None:intergenic |
| CTCATGTGATTTGAAGGACC+TGG | 0.596391 | 5.3:+1570582 | MS.gene024860:CDS |
| AAGTTTATCCCTGAGATGGT+TGG | 0.596683 | 5.3:+1570610 | MS.gene024860:CDS |
| GGCTGATCTTCAAGGAGATG+AGG | 0.598473 | 5.3:+1569735 | MS.gene024860:CDS |
| GTCTTCCAAGTGAAGAACGT+TGG | 0.603314 | 5.3:+1556908 | MS.gene024860:CDS |
| GGCGCTCTAACCAGGTCAAG+AGG | 0.606601 | 5.3:+1570046 | MS.gene024860:CDS |
| CGAATGAAAACATTCTGCAG+AGG | 0.608337 | 5.3:-1570667 | None:intergenic |
| TGACCTTGGGAAGTTGATGG+AGG | 0.615407 | 5.3:+1570720 | MS.gene024860:CDS |
| GAATGAAAACATTCTGCAGA+GGG | 0.617565 | 5.3:-1570666 | None:intergenic |
| GAGAGCACAATCATGGCGGT+CGG | 0.625040 | 5.3:+1556699 | None:intergenic |
| ATCTCAAAGGGGAAGAAGGG+TGG | 0.627186 | 5.3:+1556735 | MS.gene024860:CDS |
| TTCTCGTACTCAGGGTACCA+AGG | 0.636103 | 5.3:+1556943 | MS.gene024860:CDS |
| GAGGGAGATCATGATAAACC+AGG | 0.642253 | 5.3:+1570555 | MS.gene024860:CDS |
| TCAAGCTCCAACAATTTCTG+GGG | 0.646345 | 5.3:-1571252 | None:intergenic |
| ATACCTCCATCAACTTCCCA+AGG | 0.647762 | 5.3:-1570723 | None:intergenic |
| TCTGCATTGGGTTCACCAAG+AGG | 0.648028 | 5.3:+1570025 | MS.gene024860:CDS |
| TCTCAAAGTATAGTTATCAG+TGG | 0.652644 | 5.3:-1569998 | None:intergenic |
| CTGCAGAGGGTAGATGCTGG+AGG | 0.656069 | 5.3:-1570653 | None:intergenic |
| CAATGACCTAAGCTTGTCGG+TGG | 0.676242 | 5.3:-1569929 | None:intergenic |
| CAGGTCCTTCAAATCACATG+AGG | 0.679807 | 5.3:-1570581 | None:intergenic |
| ACCCTGAGTACGAGAAACCA+AGG | 0.681818 | 5.3:-1556936 | None:intergenic |
| ACGACCTGCAGATGAAACAA+TGG | 0.737865 | 5.3:+1571220 | MS.gene024860:CDS |
| AAAACAAGAGAATCTCAAAG+GGG | 0.749921 | 5.3:+1556724 | MS.gene024860:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 1556711 | 1571274 | 1556711 | ID=MS.gene024860 |
| chr5.3 | mRNA | 1556711 | 1571274 | 1556711 | ID=MS.gene024860.t1;Parent=MS.gene024860 |
| chr5.3 | exon | 1556711 | 1556769 | 1556711 | ID=MS.gene024860.t1.exon1;Parent=MS.gene024860.t1 |
| chr5.3 | CDS | 1556711 | 1556769 | 1556711 | ID=cds.MS.gene024860.t1;Parent=MS.gene024860.t1 |
| chr5.3 | exon | 1556859 | 1556964 | 1556859 | ID=MS.gene024860.t1.exon2;Parent=MS.gene024860.t1 |
| chr5.3 | CDS | 1556859 | 1556964 | 1556859 | ID=cds.MS.gene024860.t1;Parent=MS.gene024860.t1 |
| chr5.3 | exon | 1569691 | 1569828 | 1569691 | ID=MS.gene024860.t1.exon3;Parent=MS.gene024860.t1 |
| chr5.3 | CDS | 1569691 | 1569828 | 1569691 | ID=cds.MS.gene024860.t1;Parent=MS.gene024860.t1 |
| chr5.3 | exon | 1569916 | 1570101 | 1569916 | ID=MS.gene024860.t1.exon4;Parent=MS.gene024860.t1 |
| chr5.3 | CDS | 1569916 | 1570101 | 1569916 | ID=cds.MS.gene024860.t1;Parent=MS.gene024860.t1 |
| chr5.3 | exon | 1570541 | 1570741 | 1570541 | ID=MS.gene024860.t1.exon5;Parent=MS.gene024860.t1 |
| chr5.3 | CDS | 1570541 | 1570741 | 1570541 | ID=cds.MS.gene024860.t1;Parent=MS.gene024860.t1 |
| chr5.3 | exon | 1571179 | 1571274 | 1571179 | ID=MS.gene024860.t1.exon6;Parent=MS.gene024860.t1 |
| chr5.3 | CDS | 1571179 | 1571274 | 1571179 | ID=cds.MS.gene024860.t1;Parent=MS.gene024860.t1 |
| Gene Sequence |
| Protein sequence |
