AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49473

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49473.t1 MTR_5g006440 99.617 261 1 0 1 261 1 261 0.0 538
MS.gene49473.t1 MTR_1g103100 93.103 261 18 0 1 261 1 261 7.98e-176 484
MS.gene49473.t1 MTR_3g012040 91.188 261 23 0 1 261 1 261 1.05e-170 471
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49473.t1 AT4G34670 85.496 262 37 1 1 261 1 262 1.35e-162 451
MS.gene49473.t1 AT3G04840 85.115 262 38 1 1 261 1 262 1.44e-162 451

Find 80 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATCTCCTTACCAATTTC+TGG 0.102887 5.1:+1231275 MS.gene49473:CDS
CAACTTCCCAAGGTCAAATT+TGG 0.131525 5.1:-1232182 None:intergenic
GATCAAGCTCCAACAATTTC+TGG 0.179039 5.1:-1232571 None:intergenic
GGTCAAATTTGGGAGCTTTC+AGG 0.220044 5.1:-1232171 None:intergenic
GATATCATACCAATCCTTCT+TGG 0.266372 5.1:-1230056 None:intergenic
TTAGGTCATTGGTGCGAAAA+TGG 0.324689 5.1:+1231410 MS.gene49473:CDS
CCACTAACATACCCAGAAAT+TGG 0.324728 5.1:-1231287 None:intergenic
AACCTTGGTTTCTCGTACTC+AGG 0.324751 5.1:+1230117 MS.gene49473:CDS
GTTGAGAGCTGAAGATGTTC+AGG 0.329572 5.1:+1231249 MS.gene49473:CDS
CCTGACGGATTTGACTTGAT+TGG 0.374179 5.1:-1231549 None:intergenic
GAGATGAGGACAACGCATTC+AGG 0.376338 5.1:+1231218 MS.gene49473:CDS
CTTTGAGAATGTTCTGCATT+GGG 0.377761 5.1:+1231482 MS.gene49473:CDS
CGCCGATCCATTTGCCAAGA+AGG 0.386423 5.1:+1230042 MS.gene49473:CDS
CACCGACAAGCTTAGGTCAT+TGG 0.388612 5.1:+1231399 MS.gene49473:CDS
ATTTGACCTTGGGAAGTTGA+TGG 0.390249 5.1:+1232186 MS.gene49473:CDS
CTGACGGATTTGACTTGATT+GGG 0.398861 5.1:-1231548 None:intergenic
TCAACCATTGTTTCATCTGC+AGG 0.407580 5.1:-1232541 None:intergenic
TACCAATCCTTCTTGGCAAA+TGG 0.412105 5.1:-1230049 None:intergenic
AACTTCCCAAGGTCAAATTT+GGG 0.419750 5.1:-1232181 None:intergenic
AAATCTCCTTACCAATTTCT+GGG 0.420398 5.1:+1231276 MS.gene49473:CDS
ATCAAGCTCCAACAATTTCT+GGG 0.427325 5.1:-1232570 None:intergenic
ACAACGCATTCAGGAAGATT+AGG 0.441352 5.1:+1231227 MS.gene49473:CDS
CAGGGATAAACTTGCGTACC+AGG 0.444164 5.1:-1232069 None:intergenic
GTTTCATTGGCTGATCTTCA+AGG 0.448595 5.1:+1231196 MS.gene49473:CDS
AAGCTCCCAAATTTGACCTT+GGG 0.449450 5.1:+1232176 MS.gene49473:CDS
CACCAAGAGGCGCTCTAACC+AGG 0.467818 5.1:+1231507 MS.gene49473:CDS
AAAGCTCCCAAATTTGACCT+TGG 0.468887 5.1:+1232175 MS.gene49473:CDS
ACTTTGAGAATGTTCTGCAT+TGG 0.470669 5.1:+1231481 MS.gene49473:CDS
GAGAATCTCAAAGGGGAAGA+AGG 0.471651 5.1:+1229914 MS.gene49473:CDS
ATCCATTTGCCAAGAAGGAT+TGG 0.479936 5.1:+1230047 MS.gene49473:CDS
CACCAATGACCTAAGCTTGT+CGG 0.488210 5.1:-1231401 None:intergenic
GGCGACCTCATGTGATTTGA+AGG 0.488763 5.1:+1232045 MS.gene49473:CDS
GAAGGAACCCCAGAAATTGT+TGG 0.488913 5.1:+1232562 MS.gene49473:CDS
TCAAATCACATGAGGTCGCC+TGG 0.493748 5.1:-1232042 None:intergenic
TCGTGTGTTTGAGGTTTCAT+TGG 0.502975 5.1:+1231183 MS.gene49473:CDS
ACTCAAACATCGTGTGTTTG+AGG 0.508854 5.1:+1231174 MS.gene49473:CDS
TTCTTCACTTGGAAGACTGA+AGG 0.526406 5.1:-1230085 None:intergenic
GCAGATGAAACAATGGTTGA+AGG 0.526419 5.1:+1232544 MS.gene49473:CDS
GACTCTCATTGAAGCTCATG+TGG 0.532944 5.1:+1231435 MS.gene49473:CDS
GACCTGGTTAGAGCGCCTCT+TGG 0.533555 5.1:-1231509 None:intergenic
TCTTCACTTGGAAGACTGAA+GGG 0.535531 5.1:-1230084 None:intergenic
CCAATCAAGTCAAATCCGTC+AGG 0.535673 5.1:+1231549 MS.gene49473:CDS
GAAGAACGTTGGCAAAACCT+TGG 0.541738 5.1:+1230102 MS.gene49473:CDS
CTCTGAAGATGTTGGTACAA+AGG 0.543428 5.1:+1232510 MS.gene49473:CDS
TTGAGAGCTGAAGATGTTCA+GGG 0.547196 5.1:+1231250 MS.gene49473:CDS
ATCCTTCTTGGCAAATGGAT+CGG 0.549227 5.1:-1230044 None:intergenic
CGTAACAAGTCCTCTTGACC+TGG 0.558833 5.1:-1231525 None:intergenic
CTTCACTTGGAAGACTGAAG+GGG 0.559550 5.1:-1230083 None:intergenic
ACATATCAGATTGCTTCTGA+AGG 0.563525 5.1:+1231151 MS.gene49473:intron
ACGCAAGTTTATCCCTGAGA+TGG 0.567629 5.1:+1232075 MS.gene49473:CDS
TCAGATTCGTAGAAAGATGA+GGG 0.572813 5.1:+1232006 MS.gene49473:intron
AGAATCTCAAAGGGGAAGAA+GGG 0.576770 5.1:+1229915 MS.gene49473:CDS
ATCAGATTCGTAGAAAGATG+AGG 0.577414 5.1:+1232005 MS.gene49473:intron
ACCTTGGTTTCTCGTACTCA+GGG 0.577882 5.1:+1230118 MS.gene49473:CDS
GGAGATTACTCTGAAGATGT+TGG 0.585493 5.1:+1232502 MS.gene49473:CDS
GGTTGGAAAAGAGATTGAGA+AGG 0.589251 5.1:+1232096 MS.gene49473:CDS
AACATACCCAGAAATTGGTA+AGG 0.595805 5.1:-1231282 None:intergenic
ATTCTGCAGAGGGTAGATGC+TGG 0.596121 5.1:-1232125 None:intergenic
CTCATGTGATTTGAAGGACC+TGG 0.596391 5.1:+1232051 MS.gene49473:CDS
AAGTTTATCCCTGAGATGGT+TGG 0.596683 5.1:+1232079 MS.gene49473:CDS
GGCTGATCTTCAAGGAGATG+AGG 0.598473 5.1:+1231204 MS.gene49473:CDS
GTCTTCCAAGTGAAGAACGT+TGG 0.603314 5.1:+1230091 MS.gene49473:CDS
GGCGCTCTAACCAGGTCAAG+AGG 0.606601 5.1:+1231515 MS.gene49473:CDS
CGAATGAAAACATTCTGCAG+AGG 0.608337 5.1:-1232136 None:intergenic
TGACCTTGGGAAGTTGATGG+AGG 0.615407 5.1:+1232189 MS.gene49473:CDS
GAATGAAAACATTCTGCAGA+GGG 0.617565 5.1:-1232135 None:intergenic
GAGAGCACAATCATGGCGGT+CGG 0.625040 5.1:+1229882 None:intergenic
ATCTCAAAGGGGAAGAAGGG+TGG 0.627186 5.1:+1229918 MS.gene49473:CDS
TTCTCGTACTCAGGGTACCA+AGG 0.636103 5.1:+1230126 MS.gene49473:CDS
GAGGGAGATCATGATAAACC+AGG 0.642253 5.1:+1232024 MS.gene49473:CDS
TCAAGCTCCAACAATTTCTG+GGG 0.646345 5.1:-1232569 None:intergenic
ATACCTCCATCAACTTCCCA+AGG 0.647762 5.1:-1232192 None:intergenic
TCTGCATTGGGTTCACCAAG+AGG 0.648028 5.1:+1231494 MS.gene49473:CDS
TCTCAAAGTATAGTTATCAG+TGG 0.652644 5.1:-1231467 None:intergenic
CTGCAGAGGGTAGATGCTGG+AGG 0.656069 5.1:-1232122 None:intergenic
CAATGACCTAAGCTTGTCGG+TGG 0.676242 5.1:-1231398 None:intergenic
CAGGTCCTTCAAATCACATG+AGG 0.679807 5.1:-1232050 None:intergenic
ACCCTGAGTACGAGAAACCA+AGG 0.681818 5.1:-1230119 None:intergenic
ACGACCTGCAGATGAAACAA+TGG 0.737865 5.1:+1232537 MS.gene49473:CDS
AAAACAAGAGAATCTCAAAG+GGG 0.749921 5.1:+1229907 MS.gene49473:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTGTTGAAAAAAATTAA+TGG - chr5.1:1232350-1232369 None:intergenic 10.0%
!! AAGTTCTTTATATATTTAAC+AGG - chr5.1:1230636-1230655 None:intergenic 15.0%
!! ACTGAAAAATAACAATAATA+TGG - chr5.1:1232435-1232454 None:intergenic 15.0%
!! ATAATTATAAGGTAGAAAAT+AGG - chr5.1:1231629-1231648 None:intergenic 15.0%
!! ATTAGTGTTTAATATTGTAT+AGG + chr5.1:1230911-1230930 MS.gene49473:intron 15.0%
!! ATTAGTGTTTAATATTGTTT+AGG + chr5.1:1231027-1231046 MS.gene49473:intron 15.0%
!! CTGAAAAATAACAATAATAT+GGG - chr5.1:1232434-1232453 None:intergenic 15.0%
!!! AGTGTTAATTTATTAACTTT+TGG + chr5.1:1231059-1231078 MS.gene49473:intron 15.0%
!!! GATATAATTAAGCTATTTTT+CGG + chr5.1:1232380-1232399 MS.gene49473:intron 15.0%
!!! GTTAATTCATTGTTTATTTT+TGG + chr5.1:1232467-1232486 MS.gene49473:intron 15.0%
!! AATAGTAACTTTCATTCAAA+TGG - chr5.1:1232261-1232280 None:intergenic 20.0%
!! ATAGTAACTTTCATTCAAAT+GGG - chr5.1:1232260-1232279 None:intergenic 20.0%
!! ATTTATGAGTAAAACAACAA+GGG - chr5.1:1231861-1231880 None:intergenic 20.0%
!! CGGTATAAAAAAAAAAACAA+CGG + chr5.1:1232400-1232419 MS.gene49473:intron 20.0%
!! GAAAGAGATATACAATTATA+AGG - chr5.1:1231748-1231767 None:intergenic 20.0%
!! TATAGCTAATTAATAGCATA+TGG + chr5.1:1231717-1231736 MS.gene49473:intron 20.0%
!! TATTTATGAGTAAAACAACA+AGG - chr5.1:1231862-1231881 None:intergenic 20.0%
! AAAAACAAGAGAATCTCAAA+GGG + chr5.1:1229906-1229925 MS.gene49473:CDS 25.0%
! AAATTACAAAAGATCACACA+AGG + chr5.1:1230782-1230801 MS.gene49473:intron 25.0%
! ACATACTTGACATTATGTAT+TGG + chr5.1:1231903-1231922 MS.gene49473:intron 25.0%
! ACCAATAAGAATAAATATGC+TGG + chr5.1:1230686-1230705 MS.gene49473:intron 25.0%
! TACGAATCTGATAAAGATAA+AGG - chr5.1:1231997-1232016 None:intergenic 25.0%
! TCCAGCATATTTATTCTTAT+TGG - chr5.1:1230690-1230709 None:intergenic 25.0%
! TCGTTTAAATTTGTGAGATT+TGG + chr5.1:1230202-1230221 MS.gene49473:intron 25.0%
! TGTTATTGTGATGAATTGTT+TGG + chr5.1:1230294-1230313 MS.gene49473:intron 25.0%
!! AGTCTAAGAAACTTTTAATG+TGG - chr5.1:1230484-1230503 None:intergenic 25.0%
!! CTTATTGGTTAAATTTAAGC+TGG - chr5.1:1230675-1230694 None:intergenic 25.0%
!! TTTTCAACACATACTTCTAT+TGG + chr5.1:1230595-1230614 MS.gene49473:intron 25.0%
!!! CATTGATTTCATGTTTTTCA+CGG + chr5.1:1230520-1230539 MS.gene49473:intron 25.0%
!!! CTATGTATGTTTTTTCTACA+GGG + chr5.1:1231364-1231383 MS.gene49473:intron 25.0%
!!! TCTAATCTCTGTTTTTTCTT+AGG + chr5.1:1231927-1231946 MS.gene49473:intron 25.0%
!!! TCTATGTATGTTTTTTCTAC+AGG + chr5.1:1231363-1231382 MS.gene49473:intron 25.0%
AAAACAAGAGAATCTCAAAG+GGG + chr5.1:1229907-1229926 MS.gene49473:CDS 30.0%
AAAATCTCCTTACCAATTTC+TGG + chr5.1:1231275-1231294 MS.gene49473:CDS 30.0%
AAATCTCCTTACCAATTTCT+GGG + chr5.1:1231276-1231295 MS.gene49473:CDS 30.0%
AGCTGGTACAGATAATTATA+AGG - chr5.1:1231640-1231659 None:intergenic 30.0%
ATGAAAGAATGAGAGTGAAA+AGG - chr5.1:1229975-1229994 None:intergenic 30.0%
ATTGTAGTGAAAATACCTGA+CGG - chr5.1:1231567-1231586 None:intergenic 30.0%
CAAAAACAAGAGAATCTCAA+AGG + chr5.1:1229905-1229924 MS.gene49473:CDS 30.0%
CCATGTCAATCAAATCAAAT+TGG - chr5.1:1231101-1231120 None:intergenic 30.0%
GAAATCAATGATATGAAGTG+AGG - chr5.1:1230510-1230529 None:intergenic 30.0%
GTCAAGTATGTATCAAAACA+AGG - chr5.1:1231894-1231913 None:intergenic 30.0%
TAACCAAAACAGCTCAATAT+GGG - chr5.1:1232319-1232338 None:intergenic 30.0%
TATGTATCAAAACAAGGAGT+AGG - chr5.1:1231888-1231907 None:intergenic 30.0%
TCACAAATTTAAACGAAGCT+CGG - chr5.1:1230198-1230217 None:intergenic 30.0%
TCTCAAAGTATAGTTATCAG+TGG - chr5.1:1231470-1231489 None:intergenic 30.0%
TGAAAGAATGAGAGTGAAAA+GGG - chr5.1:1229974-1229993 None:intergenic 30.0%
TGACTAACATTCTAGTGTAA+AGG - chr5.1:1232295-1232314 None:intergenic 30.0%
TTAACCAAAACAGCTCAATA+TGG - chr5.1:1232320-1232339 None:intergenic 30.0%
TTTCACTACAATATTGCTAG+TGG + chr5.1:1231575-1231594 MS.gene49473:intron 30.0%
! AAACTTTTAATGTGGTCATC+TGG - chr5.1:1230476-1230495 None:intergenic 30.0%
! ATGAAAGAAAAAGTGTACCT+TGG - chr5.1:1230146-1230165 None:intergenic 30.0%
! TTGAGTGAATTTGTTCTTAC+TGG + chr5.1:1230965-1230984 MS.gene49473:intron 30.0%
!! CCAATTTGATTTGATTGACA+TGG + chr5.1:1231098-1231117 MS.gene49473:intron 30.0%
!! TCTCTTTGCATTTTAGCAAT+TGG + chr5.1:1230720-1230739 MS.gene49473:intron 30.0%
!!! CATTGTTTATTTTTGGTCGT+AGG + chr5.1:1232474-1232493 MS.gene49473:intron 30.0%
!!! CTCTGTTTTTTCTTAGGAAA+TGG + chr5.1:1231933-1231952 MS.gene49473:intron 30.0%
!!! GTATCAGTTGAGTCATTTTT+TGG + chr5.1:1230748-1230767 MS.gene49473:intron 30.0%
!!! TATGTTTTTTCTACAGGGAA+TGG + chr5.1:1231369-1231388 MS.gene49473:intron 30.0%
!!! TGAGTCATTTTTTGGTACAA+AGG + chr5.1:1230756-1230775 MS.gene49473:intron 30.0%
AACATACCCAGAAATTGGTA+AGG - chr5.1:1231285-1231304 None:intergenic 35.0%
AACTTCCCAAGGTCAAATTT+GGG - chr5.1:1232184-1232203 None:intergenic 35.0%
AATTATCTGTACCAGCTTGT+AGG + chr5.1:1231643-1231662 MS.gene49473:intron 35.0%
ACATATCAGATTGCTTCTGA+AGG + chr5.1:1231151-1231170 MS.gene49473:intron 35.0%
ATCAGATTCGTAGAAAGATG+AGG + chr5.1:1232005-1232024 MS.gene49473:intron 35.0%
ATCATACCCATTGGCATTAT+GGG - chr5.1:1230357-1230376 None:intergenic 35.0%
CCACAAAATATCATACCCAT+TGG - chr5.1:1230366-1230385 None:intergenic 35.0%
GAATGAAAACATTCTGCAGA+GGG - chr5.1:1232138-1232157 None:intergenic 35.0%
GATATCATACCAATCCTTCT+TGG - chr5.1:1230059-1230078 None:intergenic 35.0%
TATCATACCCATTGGCATTA+TGG - chr5.1:1230358-1230377 None:intergenic 35.0%
TATGAGTAAAACAACAAGGG+TGG - chr5.1:1231858-1231877 None:intergenic 35.0%
TCAGATTCGTAGAAAGATGA+GGG + chr5.1:1232006-1232025 MS.gene49473:intron 35.0%
TTCAAATGGGAACAAAGTAG+TGG - chr5.1:1232247-1232266 None:intergenic 35.0%
! ACTTTGAGAATGTTCTGCAT+TGG + chr5.1:1231481-1231500 MS.gene49473:CDS 35.0%
! CCAATGGGTATGATATTTTG+TGG + chr5.1:1230363-1230382 MS.gene49473:intron 35.0%
! CTTTGAGAATGTTCTGCATT+GGG + chr5.1:1231482-1231501 MS.gene49473:CDS 35.0%
!! CACAAGGTATTTTCTGTTCA+AGG + chr5.1:1230798-1230817 MS.gene49473:intron 35.0%
!!! TATTTTTGGTCGTAGGTTCA+TGG + chr5.1:1232481-1232500 MS.gene49473:intron 35.0%
AAAGCTCCCAAATTTGACCT+TGG + chr5.1:1232175-1232194 MS.gene49473:CDS 40.0%
AAGCTCCCAAATTTGACCTT+GGG + chr5.1:1232176-1232195 MS.gene49473:CDS 40.0%
AAGTTTATCCCTGAGATGGT+TGG + chr5.1:1232079-1232098 MS.gene49473:CDS 40.0%
ACAACGCATTCAGGAAGATT+AGG + chr5.1:1231227-1231246 MS.gene49473:CDS 40.0%
ACACATAGATACCACACAGT+AGG - chr5.1:1230250-1230269 None:intergenic 40.0%
ACTCAAACATCGTGTGTTTG+AGG + chr5.1:1231174-1231193 MS.gene49473:CDS 40.0%
AGAATCTCAAAGGGGAAGAA+GGG + chr5.1:1229915-1229934 MS.gene49473:CDS 40.0%
AGCTTATCCTCATTCCATCT+AGG - chr5.1:1230878-1230897 None:intergenic 40.0%
ATCCATTTGCCAAGAAGGAT+TGG + chr5.1:1230047-1230066 MS.gene49473:CDS 40.0%
ATCCTTCTTGGCAAATGGAT+CGG - chr5.1:1230047-1230066 None:intergenic 40.0%
ATTTGACCTTGGGAAGTTGA+TGG + chr5.1:1232186-1232205 MS.gene49473:CDS 40.0%
CAACTTCCCAAGGTCAAATT+TGG - chr5.1:1232185-1232204 None:intergenic 40.0%
CCAATTAACATCCTACAAGC+TGG - chr5.1:1231657-1231676 None:intergenic 40.0%
CCACTAACATACCCAGAAAT+TGG - chr5.1:1231290-1231309 None:intergenic 40.0%
CCAGCTTGTAGGATGTTAAT+TGG + chr5.1:1231654-1231673 MS.gene49473:intron 40.0%
CGAATGAAAACATTCTGCAG+AGG - chr5.1:1232139-1232158 None:intergenic 40.0%
GCAGATGAAACAATGGTTGA+AGG + chr5.1:1232544-1232563 MS.gene49473:CDS 40.0%
GGTTGGAAAAGAGATTGAGA+AGG + chr5.1:1232096-1232115 MS.gene49473:CDS 40.0%
TACCAATCCTTCTTGGCAAA+TGG - chr5.1:1230052-1230071 None:intergenic 40.0%
TCAACCATTGTTTCATCTGC+AGG - chr5.1:1232544-1232563 None:intergenic 40.0%
TCAAGCTCCAACAATTTCTG+GGG - chr5.1:1232572-1232591 None:intergenic 40.0%
TCTTCACTTGGAAGACTGAA+GGG - chr5.1:1230087-1230106 None:intergenic 40.0%
TGTGTGGTATCTATGTGTTG+TGG + chr5.1:1230252-1230271 MS.gene49473:intron 40.0%
TTAGGTCATTGGTGCGAAAA+TGG + chr5.1:1231410-1231429 MS.gene49473:CDS 40.0%
TTCTTCACTTGGAAGACTGA+AGG - chr5.1:1230088-1230107 None:intergenic 40.0%
TTGAGAGCTGAAGATGTTCA+GGG + chr5.1:1231250-1231269 MS.gene49473:CDS 40.0%
! AGTGGTTTCAAGTCTAGTAG+TGG - chr5.1:1232229-1232248 None:intergenic 40.0%
! ATCCTCATTCCATCTAGGAT+AGG - chr5.1:1230873-1230892 None:intergenic 40.0%
! ATCTCTTTTCCAACCATCTC+AGG - chr5.1:1232091-1232110 None:intergenic 40.0%
! ATTTTACCACCGACAAGCTT+AGG + chr5.1:1231392-1231411 MS.gene49473:CDS 40.0%
! CAACCCATATTGAGCTGTTT+TGG + chr5.1:1232313-1232332 MS.gene49473:intron 40.0%
! CCAATTTCTGGGTATGTTAG+TGG + chr5.1:1231287-1231306 MS.gene49473:intron 40.0%
! CTCTGAAGATGTTGGTACAA+AGG + chr5.1:1232510-1232529 MS.gene49473:CDS 40.0%
! GATTTCATGTTTTTCACGGC+TGG + chr5.1:1230524-1230543 MS.gene49473:intron 40.0%
! GGAGATTACTCTGAAGATGT+TGG + chr5.1:1232502-1232521 MS.gene49473:CDS 40.0%
! GTTTCATTGGCTGATCTTCA+AGG + chr5.1:1231196-1231215 MS.gene49473:CDS 40.0%
! TCCTCATTCCATCTAGGATA+GGG - chr5.1:1230872-1230891 None:intergenic 40.0%
! TCGTGTGTTTGAGGTTTCAT+TGG + chr5.1:1231183-1231202 MS.gene49473:CDS 40.0%
! TCTCTTTTCCAACCATCTCA+GGG - chr5.1:1232090-1232109 None:intergenic 40.0%
! TTTTGCCAACGTTCTTCACT+TGG - chr5.1:1230099-1230118 None:intergenic 40.0%
!! CTGACGGATTTGACTTGATT+GGG - chr5.1:1231551-1231570 None:intergenic 40.0%
AACCTTGGTTTCTCGTACTC+AGG + chr5.1:1230117-1230136 MS.gene49473:CDS 45.0%
ACCTTGGTTTCTCGTACTCA+GGG + chr5.1:1230118-1230137 MS.gene49473:CDS 45.0%
ACGACCTGCAGATGAAACAA+TGG + chr5.1:1232537-1232556 MS.gene49473:CDS 45.0%
ACGCAAGTTTATCCCTGAGA+TGG + chr5.1:1232075-1232094 MS.gene49473:CDS 45.0%
ACTGATCTCTTCCTACTGTG+TGG + chr5.1:1230236-1230255 MS.gene49473:intron 45.0%
ACTGTGTCCCATAATGCCAA+TGG + chr5.1:1230347-1230366 MS.gene49473:intron 45.0%
ATACCTCCATCAACTTCCCA+AGG - chr5.1:1232195-1232214 None:intergenic 45.0%
CACCAATGACCTAAGCTTGT+CGG - chr5.1:1231404-1231423 None:intergenic 45.0%
CAGGTCCTTCAAATCACATG+AGG - chr5.1:1232053-1232072 None:intergenic 45.0%
CCAATCAAGTCAAATCCGTC+AGG + chr5.1:1231549-1231568 MS.gene49473:CDS 45.0%
CTCATGTGATTTGAAGGACC+TGG + chr5.1:1232051-1232070 MS.gene49473:CDS 45.0%
CTGTGTCCCATAATGCCAAT+GGG + chr5.1:1230348-1230367 MS.gene49473:intron 45.0%
CTTCACTTGGAAGACTGAAG+GGG - chr5.1:1230086-1230105 None:intergenic 45.0%
GAAGAACGTTGGCAAAACCT+TGG + chr5.1:1230102-1230121 MS.gene49473:CDS 45.0%
GAAGGAACCCCAGAAATTGT+TGG + chr5.1:1232562-1232581 MS.gene49473:CDS 45.0%
GACTCTCATTGAAGCTCATG+TGG + chr5.1:1231435-1231454 MS.gene49473:CDS 45.0%
GAGAATCTCAAAGGGGAAGA+AGG + chr5.1:1229914-1229933 MS.gene49473:CDS 45.0%
GAGGGAGATCATGATAAACC+AGG + chr5.1:1232024-1232043 MS.gene49473:CDS 45.0%
GGTCAAATTTGGGAGCTTTC+AGG - chr5.1:1232174-1232193 None:intergenic 45.0%
GTCTTCCAAGTGAAGAACGT+TGG + chr5.1:1230091-1230110 MS.gene49473:CDS 45.0%
GTTGAGAGCTGAAGATGTTC+AGG + chr5.1:1231249-1231268 MS.gene49473:CDS 45.0%
! AAAATGCAAAGAGAGCGCAC+TGG - chr5.1:1230714-1230733 None:intergenic 45.0%
!! AGTCAGCTTTGGGAATTCAC+AGG - chr5.1:1230835-1230854 None:intergenic 45.0%
!! CCTGACGGATTTGACTTGAT+TGG - chr5.1:1231552-1231571 None:intergenic 45.0%
ACCCTGAGTACGAGAAACCA+AGG - chr5.1:1230122-1230141 None:intergenic 50.0%
ACCTCTTGCCCTATCCTAGA+TGG + chr5.1:1230861-1230880 MS.gene49473:intron 50.0%
AGGTGGAGCAAGTCAGCTTT+GGG - chr5.1:1230845-1230864 None:intergenic 50.0%
ATCTCAAAGGGGAAGAAGGG+TGG + chr5.1:1229918-1229937 MS.gene49473:CDS 50.0%
ATTCTGCAGAGGGTAGATGC+TGG - chr5.1:1232128-1232147 None:intergenic 50.0%
CAATGACCTAAGCTTGTCGG+TGG - chr5.1:1231401-1231420 None:intergenic 50.0%
CACCGACAAGCTTAGGTCAT+TGG + chr5.1:1231399-1231418 MS.gene49473:CDS 50.0%
CAGGGATAAACTTGCGTACC+AGG - chr5.1:1232072-1232091 None:intergenic 50.0%
CGTAACAAGTCCTCTTGACC+TGG - chr5.1:1231528-1231547 None:intergenic 50.0%
GAGATGAGGACAACGCATTC+AGG + chr5.1:1231218-1231237 MS.gene49473:CDS 50.0%
GCCCTATCCTAGATGGAATG+AGG + chr5.1:1230868-1230887 MS.gene49473:intron 50.0%
GGCGACCTCATGTGATTTGA+AGG + chr5.1:1232045-1232064 MS.gene49473:CDS 50.0%
GGCTGATCTTCAAGGAGATG+AGG + chr5.1:1231204-1231223 MS.gene49473:CDS 50.0%
TCAAATCACATGAGGTCGCC+TGG - chr5.1:1232045-1232064 None:intergenic 50.0%
TCTGCATTGGGTTCACCAAG+AGG + chr5.1:1231494-1231513 MS.gene49473:CDS 50.0%
! ATCTAGGATAGGGCAAGAGG+TGG - chr5.1:1230862-1230881 None:intergenic 50.0%
! TCCATCTAGGATAGGGCAAG+AGG - chr5.1:1230865-1230884 None:intergenic 50.0%
! TTCTCGTACTCAGGGTACCA+AGG + chr5.1:1230126-1230145 MS.gene49473:CDS 50.0%
!! CAGCTTTGGGAATTCACAGG+TGG - chr5.1:1230832-1230851 None:intergenic 50.0%
!! TGACCTTGGGAAGTTGATGG+AGG + chr5.1:1232189-1232208 MS.gene49473:CDS 50.0%
CGCCGATCCATTTGCCAAGA+AGG + chr5.1:1230042-1230061 MS.gene49473:CDS 55.0%
GAGGTGGAGCAAGTCAGCTT+TGG - chr5.1:1230846-1230865 None:intergenic 55.0%
CTGCAGAGGGTAGATGCTGG+AGG - chr5.1:1232125-1232144 None:intergenic 60.0%
GGCGCTCTAACCAGGTCAAG+AGG + chr5.1:1231515-1231534 MS.gene49473:CDS 60.0%
! GACCTGGTTAGAGCGCCTCT+TGG - chr5.1:1231512-1231531 None:intergenic 60.0%
!! CACCAAGAGGCGCTCTAACC+AGG + chr5.1:1231507-1231526 MS.gene49473:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 1229894 1232591 1229894 ID=MS.gene49473
chr5.1 mRNA 1229894 1232591 1229894 ID=MS.gene49473.t1;Parent=MS.gene49473
chr5.1 exon 1229894 1229952 1229894 ID=MS.gene49473.t1.exon1;Parent=MS.gene49473.t1
chr5.1 CDS 1229894 1229952 1229894 ID=cds.MS.gene49473.t1;Parent=MS.gene49473.t1
chr5.1 exon 1230042 1230147 1230042 ID=MS.gene49473.t1.exon2;Parent=MS.gene49473.t1
chr5.1 CDS 1230042 1230147 1230042 ID=cds.MS.gene49473.t1;Parent=MS.gene49473.t1
chr5.1 exon 1231160 1231297 1231160 ID=MS.gene49473.t1.exon3;Parent=MS.gene49473.t1
chr5.1 CDS 1231160 1231297 1231160 ID=cds.MS.gene49473.t1;Parent=MS.gene49473.t1
chr5.1 exon 1231385 1231570 1231385 ID=MS.gene49473.t1.exon4;Parent=MS.gene49473.t1
chr5.1 CDS 1231385 1231570 1231385 ID=cds.MS.gene49473.t1;Parent=MS.gene49473.t1
chr5.1 exon 1232010 1232210 1232010 ID=MS.gene49473.t1.exon5;Parent=MS.gene49473.t1
chr5.1 CDS 1232010 1232210 1232010 ID=cds.MS.gene49473.t1;Parent=MS.gene49473.t1
chr5.1 exon 1232496 1232591 1232496 ID=MS.gene49473.t1.exon6;Parent=MS.gene49473.t1
chr5.1 CDS 1232496 1232591 1232496 ID=cds.MS.gene49473.t1;Parent=MS.gene49473.t1
Gene Sequence

>MS.gene49473

ATGGCGGTCGGCAAAAACAAGAGAATCTCAAAGGGGAAGAAGGGTGGCAAGAAGAAAGCGTAAGCTTTTCATTCATTCCCTTTTCACTCTCATTCTTTCATTTTTGTTATTTAGCTCAGATTTTTTTGATTCAATTATCAATTTACAGCGCCGATCCATTTGCCAAGAAGGATTGGTATGATATCAAAGCCCCTTCAGTCTTCCAAGTGAAGAACGTTGGCAAAACCTTGGTTTCTCGTACTCAGGGTACCAAGGTACACTTTTTCTTTCATATTCTGTATCAAAATTTATAATATTTTAACCGAGCTTCGTTTAAATTTGTGAGATTTGGTTATTTTGTTTACTGATCTCTTCCTACTGTGTGGTATCTATGTGTTGTGGAATTTAGATTGCTCTTAAATGTTATTGTGATGAATTGTTTGGCAATATAGTTTATAGAACTAATGTTGTTATACTGTGTCCCATAATGCCAATGGGTATGATATTTTGTGGTTTTTAATTTAATTTTTTTTATCTTATCTGAAGAACATTAACAATGAATATAGTCTATAAAATGAATGCTTATATGCTTCATTAATACCAGATGACCACATTAAAAGTTTCTTAGACTAGACCTCACTTCATATCATTGATTTCATGTTTTTCACGGCTGGAAATAAAGAATGAAAATTGCTAGCAACATACTACTCACTATTACAATCTTTTCAACACATACTTCTATTGGTTGAAATTTATAAATCCTGTTAAATATATAAAGAACTTGCATAATTAAGTGAGACCAGCTTAAATTTAACCAATAAGAATAAATATGCTGGAACCAGTGCGCTCTCTTTGCATTTTAGCAATTGGCAAATGTATCAGTTGAGTCATTTTTTGGTACAAAGGTAAAAATTACAAAAGATCACACAAGGTATTTTCTGTTCAAGGATGCAATTCCACCTGTGAATTCCCAAAGCTGACTTGCTCCACCTCTTGCCCTATCCTAGATGGAATGAGGATAAGCTTAGATCATGATTAATTAGTGTTTAATATTGTATAGGAGTTTTATATTATTTTTTTAAAGTTATAGTTTTGAGTGAATTTGTTCTTACTGGCAATGTAATTTTTTGACTTGATTTTCTAGTTCATGATTAATTAGTGTTTAATATTGTTTAGGAGTCTTAAGAGTGTTAATTTATTAACTTTTGGTGTATTTGTTGTATGTCCAATTTGATTTGATTGACATGGTTATCTTAATGATGATTCTATGTTGTTTTTACATATCAGATTGCTTCTGAAGGACTCAAACATCGTGTGTTTGAGGTTTCATTGGCTGATCTTCAAGGAGATGAGGACAACGCATTCAGGAAGATTAGGTTGAGAGCTGAAGATGTTCAGGGAAAAAATCTCCTTACCAATTTCTGGGTATGTTAGTGGCTGTGTATTTTTTTTATTTTGTTTTATTGTTGCATGAAAAGTGTATGATGTATTCTATGTATGTTTTTTCTACAGGGAATGGATTTTACCACCGACAAGCTTAGGTCATTGGTGCGAAAATGGCAGACTCTCATTGAAGCTCATGTGGATGTTAAGACCACTGATAACTATACTTTGAGAATGTTCTGCATTGGGTTCACCAAGAGGCGCTCTAACCAGGTCAAGAGGACTTGTTACGCCCAATCAAGTCAAATCCGTCAGGTATTTTCACTACAATATTGCTAGTGGTTTCATTTGAACTCTGCTTTGTCTTCAACCTATTTTCTACCTTATAATTATCTGTACCAGCTTGTAGGATGTTAATTGGTGTACACTGTAATTGCATATCTCTTTTAGTCAAGCACTTATATAGCTAATTAATAGCATATGGTGTGACCTTATAATTGTATATCTCTTTCAATCAAGCACATATAACTAATTTATAGTGTATGTAGTAAATTAAGTTATATCTTTACTAGTTAATTTTTCTCTCTGATTTTGCATATCCACCCTTGTTGTTTTACTCATAAATATCTCCTACTCCTTGTTTTGATACATACTTGACATTATGTATTGGTTCTAATCTCTGTTTTTTCTTAGGAAATGGTTTGTTATGTCAGATAGCATAAAAAGCTTATATGTTTTCCTTTATCTTTATCAGATTCGTAGAAAGATGAGGGAGATCATGATAAACCAGGCGACCTCATGTGATTTGAAGGACCTGGTACGCAAGTTTATCCCTGAGATGGTTGGAAAAGAGATTGAGAAGGCTACCTCCAGCATCTACCCTCTGCAGAATGTTTTCATTCGTAAAGTTAAGATCCTGAAAGCTCCCAAATTTGACCTTGGGAAGTTGATGGAGGTATGTCTTTTATGTCCACTACTAGACTTGAAACCACTACTTTGTTCCCATTTGAATGAAAGTTACTATTATTTAATGATTCCTTTACACTAGAATGTTAGTCAACCCATATTGAGCTGTTTTGGTTAAAGATTTTCCATTAATTTTTTTCAACAAATTATGATCGTAAGATATAATTAAGCTATTTTTCGGTATAAAAAAAAAAACAACGGTTGAATGACCCATATTATTGTTATTTTTCAGTTTTCGTCAGTTTGTTAATTCATTGTTTATTTTTGGTCGTAGGTTCATGGAGATTACTCTGAAGATGTTGGTACAAAGGTTGAACGACCTGCAGATGAAACAATGGTTGAAGGAACCCCAGAAATTGTTGGAGCTTGA
Protein sequence

>MS.gene49473.t1

MAVGKNKRISKGKKGGKKKAADPFAKKDWYDIKAPSVFQVKNVGKTLVSRTQGTKIASEGLKHRVFEVSLADLQGDEDNAFRKIRLRAEDVQGKNLLTNFWGMDFTTDKLRSLVRKWQTLIEAHVDVKTTDNYTLRMFCIGFTKRRSNQVKRTCYAQSSQIRQIRRKMREIMINQATSCDLKDLVRKFIPEMVGKEIEKATSSIYPLQNVFIRKVKILKAPKFDLGKLMEVHGDYSEDVGTKVERPADETMVEGTPEIVGA