AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77160.t1 MTR_1g103100 97.701 261 6 0 1 261 1 261 0.0 530
MS.gene77160.t1 MTR_3g012040 95.785 261 11 0 1 261 1 261 0.0 497
MS.gene77160.t1 MTR_5g006440 94.636 261 14 0 1 261 1 261 9.71e-179 492
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77160.t1 AT3G04840 85.115 262 38 1 1 261 1 262 1.68e-163 453
MS.gene77160.t1 AT4G34670 85.496 262 37 1 1 261 1 262 4.36e-163 452

Find 78 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCAAACTTGGGAGCTTTA+AGG 0.168578 2.4:+32010251 None:intergenic
AATATCATACCAATCCTTCT+TGG 0.295389 2.4:+32012244 None:intergenic
GAAGGATTGGTATGATATTA+AGG 0.312711 2.4:-32012240 MS.gene77160:CDS
CTGACTAGATTGTGCATAAC+AGG 0.339955 2.4:+32010667 None:intergenic
ATTTGGTCCTTGTAGGTTCA+TGG 0.344435 2.4:-32009629 MS.gene77160:intron
AAAGCTCCCAAGTTTGATCT+TGG 0.357514 2.4:-32010247 MS.gene77160:CDS
TAAGAACAAGCGGATCTCTA+AGG 0.365175 2.4:-32012397 MS.gene77160:CDS
GTTTGATCTTGGAAAATTGA+TGG 0.369245 2.4:-32010236 MS.gene77160:CDS
AAATTCATTCCCGAGATGAT+TGG 0.381702 2.4:-32010343 MS.gene77160:CDS
GGGACGAAGACCATGCATTC+AGG 0.393353 2.4:-32010992 MS.gene77160:CDS
ATCCTGTGATTTGAAGGAAT+TGG 0.402781 2.4:-32010371 MS.gene77160:CDS
GACCCTCGTGTCTCGTACTC+AGG 0.406163 2.4:-32012183 MS.gene77160:CDS
TACCAATCCTTCTTGGCAAA+TGG 0.412105 2.4:+32012251 None:intergenic
TAGAGTGTTTGAGGTTTCAT+TGG 0.413434 2.4:-32011027 MS.gene77160:CDS
GTTTCATTGGCTGATCTTCA+AGG 0.434675 2.4:-32011014 MS.gene77160:CDS
TGCTGATCCATTTGCCAAGA+AGG 0.436339 2.4:-32012258 MS.gene77160:CDS
AATTCATTCCCGAGATGATT+GGG 0.437177 2.4:-32010342 MS.gene77160:CDS
AGCATCATCCTGTGATTTGA+AGG 0.439297 2.4:-32010377 MS.gene77160:CDS
TACCAAGAGGAGAGCTAACC+AGG 0.440122 2.4:-32010699 MS.gene77160:CDS
TCCGTCACCATTTCATCAGC+AGG 0.444686 2.4:+32009569 None:intergenic
ACTCAAGCATAGAGTGTTTG+AGG 0.454600 2.4:-32011036 MS.gene77160:CDS
ACCATGCATTCAGGAAGATT+AGG 0.462005 2.4:-32010983 MS.gene77160:CDS
ATCCATTTGCCAAGAAGGAT+TGG 0.465718 2.4:-32012253 MS.gene77160:CDS
ATCTCCTTCCCAATCATCTC+GGG 0.483111 2.4:+32010334 None:intergenic
GAGGAACCTACTGAAATTGT+TGG 0.484047 2.4:-32009548 MS.gene77160:CDS
TGTTCAGATCCGTAGGAAGA+TGG 0.490433 2.4:-32010419 MS.gene77160:intron
CACCTGGTTAGCTCTCCTCT+TGG 0.502596 2.4:+32010697 None:intergenic
AACTGACAAACTGAGGTCAT+TGG 0.506332 2.4:-32010807 MS.gene77160:CDS
TGAGGTCATTGGTGCGTAAG+TGG 0.507827 2.4:-32010796 MS.gene77160:CDS
CGGATCTCTAAGGGGAAGAA+AGG 0.516719 2.4:-32012387 MS.gene77160:CDS
AAGAACAAGCGGATCTCTAA+GGG 0.521243 2.4:-32012396 MS.gene77160:CDS
CTTGTTCTTACCAACGGCCA+TGG 0.523346 2.4:+32012410 None:intergenic
ACCTAATCTTCCTGAATGCA+TGG 0.523822 2.4:+32010982 None:intergenic
TTTCATTGGCTGATCTTCAA+GGG 0.523908 2.4:-32011013 MS.gene77160:CDS
TCATCTCGGGAATGAATTTG+CGG 0.528925 2.4:+32010347 None:intergenic
AGAGAGGCCTGCTGATGAAA+TGG 0.532099 2.4:-32009576 MS.gene77160:CDS
TCTTGCCGACATTCTTCACC+TGG 0.534041 2.4:+32012204 None:intergenic
AATCTCCTTCCCAATCATCT+CGG 0.535678 2.4:+32010333 None:intergenic
TTGAGAGCTGAAGATGTTCA+AGG 0.537527 2.4:-32010960 MS.gene77160:CDS
CGGATGAACACATTCTGCAA+GGG 0.539130 2.4:+32010286 None:intergenic
TCAAATCACAGGATGATGCT+TGG 0.544201 2.4:+32010380 None:intergenic
CATAACAGGTCCTCTTCACC+TGG 0.545447 2.4:+32010681 None:intergenic
GGAGAGCTAACCAGGTGAAG+AGG 0.557404 2.4:-32010691 MS.gene77160:CDS
GAATGACAGATTGCTTCAGA+TGG 0.560187 2.4:-32011059 MS.gene77160:intron
CTCTGAGGATGTTGGCACAA+AGG 0.562498 2.4:-32009600 MS.gene77160:CDS
CTTTATTTGTTCAGATCCGT+AGG 0.563835 2.4:-32010426 MS.gene77160:intron
GATCCGCTTGTTCTTACCAA+CGG 0.569816 2.4:+32012404 None:intergenic
GACCAATTCCTTCAAATCAC+AGG 0.574407 2.4:+32010369 None:intergenic
TCGGATGAACACATTCTGCA+AGG 0.575354 2.4:+32010285 None:intergenic
ACAATCTAGTCAGATTAGAC+AGG 0.580594 2.4:-32010657 MS.gene77160:intron
CAAGCTCCAACAATTTCAGT+AGG 0.582102 2.4:+32009542 None:intergenic
GGAGATTACTCTGAGGATGT+TGG 0.587473 2.4:-32009608 MS.gene77160:CDS
TTCTTCACCTGGAAAACTGA+AGG 0.588083 2.4:+32012215 None:intergenic
TGATCTCAACCATCTTCCTA+CGG 0.590794 2.4:+32010410 None:intergenic
TTCATTGGCTGATCTTCAAG+GGG 0.595752 2.4:-32011012 MS.gene77160:CDS
CAGTAGTCTTGACATCCACA+TGG 0.596992 2.4:+32010756 None:intergenic
ACTCTGCAAACCATGGCCGT+TGG 0.603780 2.4:-32012420 None:intergenic
CATTCCCGAGATGATTGGGA+AGG 0.605231 2.4:-32010338 MS.gene77160:CDS
TGATCTTGGAAAATTGATGG+AGG 0.612140 2.4:-32010233 MS.gene77160:intron
TGAGAGCTGAAGATGTTCAA+GGG 0.612312 2.4:-32010959 MS.gene77160:CDS
ACCCTCGTGTCTCGTACTCA+GGG 0.615233 2.4:-32012182 MS.gene77160:CDS
ATCTCTAAGGGGAAGAAAGG+TGG 0.621403 2.4:-32012384 MS.gene77160:CDS
AACTCTTGTTGAAGCCCATG+TGG 0.622205 2.4:-32010771 MS.gene77160:CDS
GTCTCGTACTCAGGGTACCA+AGG 0.623684 2.4:-32012174 MS.gene77160:intron
TGGCCGTTGGTAAGAACAAG+CGG 0.624911 2.4:-32012407 MS.gene77160:CDS
GCCTGCTGATGAAATGGTGA+CGG 0.629382 2.4:-32009570 MS.gene77160:CDS
GGATGAACACATTCTGCAAG+GGG 0.631103 2.4:+32010287 None:intergenic
CAAGGGGTAAATACTAGACG+TGG 0.633754 2.4:+32010303 None:intergenic
CTACTGATAATTACACATTG+AGG 0.636083 2.4:-32010739 MS.gene77160:CDS
TTTGCATTGGCTTTACCAAG+AGG 0.642280 2.4:-32010712 MS.gene77160:CDS
TACCCTGAGTACGAGACACG+AGG 0.648373 2.4:+32012180 None:intergenic
ATGTTGGCACAAAGGTAGAG+AGG 0.654238 2.4:-32009592 MS.gene77160:CDS
ATTTCACAACTGACAAACTG+AGG 0.681977 2.4:-32010814 MS.gene77160:CDS
TGCTGATGAAATGGTGACGG+AGG 0.686062 2.4:-32009567 MS.gene77160:CDS
ACCCTGAGTACGAGACACGA+GGG 0.700568 2.4:+32012181 None:intergenic
AGTAGTCTTGACATCCACAT+GGG 0.706350 2.4:+32010757 None:intergenic
AGAACAAGCGGATCTCTAAG+GGG 0.721950 2.4:-32012395 MS.gene77160:CDS
GGTTCATGGAGATTACTCTG+AGG 0.741731 2.4:-32009615 MS.gene77160:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATATTATGAAAATAAATAT+TGG + chr2.4:32011785-32011804 None:intergenic 10.0%
!!! ATTTTTTTTTTTATTTACAG+GGG - chr2.4:32011108-32011127 MS.gene77160:intron 10.0%
!!! GATTTTTTTTTTTATTTACA+GGG - chr2.4:32011107-32011126 MS.gene77160:intron 10.0%
!!! TGATTTTTTTTTTTATTTAC+AGG - chr2.4:32011106-32011125 MS.gene77160:intron 10.0%
!! GTAAAAAATGAAAATTTGAA+TGG + chr2.4:32009628-32009647 None:intergenic 15.0%
!!! GTTTTTTGTTTTTTGTAATT+TGG - chr2.4:32009817-32009836 MS.gene77160:intron 15.0%
!!! TAAGAGAATATTTCTTTTTT+TGG + chr2.4:32012068-32012087 None:intergenic 15.0%
!!! TTGGTATAAAAATTGTTTTA+TGG + chr2.4:32010356-32010375 None:intergenic 15.0%
!! ATATATAAAGAAGAAATCAC+TGG - chr2.4:32011435-32011454 MS.gene77160:intron 20.0%
!! ATATTCAATGAAAGTAAGAT+AGG + chr2.4:32009886-32009905 None:intergenic 20.0%
!! CTTAATGCTTATTTAGAAAA+TGG - chr2.4:32010860-32010879 MS.gene77160:intron 20.0%
!!! AAAATCTTTTGACAAATTTC+TGG - chr2.4:32011014-32011033 MS.gene77160:CDS 20.0%
!!! AAATCTTTTGACAAATTTCT+GGG - chr2.4:32011015-32011034 MS.gene77160:CDS 20.0%
!!! ATTTGAATGGATTTTGAAAA+AGG + chr2.4:32009615-32009634 None:intergenic 20.0%
!!! CAATACATTATTTCTAGTTT+TGG - chr2.4:32012144-32012163 MS.gene77160:intron 20.0%
!!! TCTTGTCTTTTAAATAATGT+TGG + chr2.4:32010558-32010577 None:intergenic 20.0%
!!! TTTGAATGGATTTTGAAAAA+GGG + chr2.4:32009614-32009633 None:intergenic 20.0%
! AAAAACAAAAAACATGACAG+AGG + chr2.4:32009810-32009829 None:intergenic 25.0%
! AAACCATTATAGAAAATGCA+AGG + chr2.4:32009657-32009676 None:intergenic 25.0%
! AACAGCATTTCATAAAAGAA+AGG + chr2.4:32011415-32011434 None:intergenic 25.0%
! AAGAAAATGTTGTCAAATAG+CGG + chr2.4:32010318-32010337 None:intergenic 25.0%
! AATAATCTGAAGGTTGTTTA+TGG + chr2.4:32011924-32011943 None:intergenic 25.0%
! ACAACATTATGACAAATAGA+TGG + chr2.4:32011058-32011077 None:intergenic 25.0%
! ACTTATAACCATCTAAAAAG+TGG - chr2.4:32012276-32012295 MS.gene77160:intron 25.0%
! ATATATTAGTGTATCCAACA+CGG - chr2.4:32011364-32011383 MS.gene77160:intron 25.0%
! ATTAAGCTATCTAAACAATC+AGG + chr2.4:32009919-32009938 None:intergenic 25.0%
! ATTATGACAAATAGATGGTT+CGG + chr2.4:32011053-32011072 None:intergenic 25.0%
! GAGAATAAAAATGAAGACAT+GGG + chr2.4:32010661-32010680 None:intergenic 25.0%
! TATCACCTGAATAAAAACTT+TGG - chr2.4:32011801-32011820 MS.gene77160:intron 25.0%
! TCTTACTTTCATTGAATATC+TGG - chr2.4:32009887-32009906 MS.gene77160:intron 25.0%
! TTAAGCTATCTAAACAATCA+GGG + chr2.4:32009918-32009937 None:intergenic 25.0%
! TTATTTCCATAATAGCCTAT+AGG + chr2.4:32010051-32010070 None:intergenic 25.0%
! TTGAACTTGTAATAATCTGA+AGG + chr2.4:32011934-32011953 None:intergenic 25.0%
!! AAAAACTTTGGTTGTTACTT+TGG - chr2.4:32011813-32011832 MS.gene77160:intron 25.0%
!! AAATATGTCTTAGTCTTTCA+TGG - chr2.4:32010802-32010821 MS.gene77160:CDS 25.0%
!! AACAACCAAAGTTTTTATTC+AGG + chr2.4:32011809-32011828 None:intergenic 25.0%
!! AAGCTAAAAGTTTTTCCTAT+AGG - chr2.4:32010033-32010052 MS.gene77160:intron 25.0%
!! AATATGTCTTAGTCTTTCAT+GGG - chr2.4:32010803-32010822 MS.gene77160:CDS 25.0%
!! AATTTTCCAAGATCAAACTT+GGG + chr2.4:32011711-32011730 None:intergenic 25.0%
!! TAACACTACCCAATTTTAAA+AGG + chr2.4:32012014-32012033 None:intergenic 25.0%
!! TTTCCTTGCATTTTCTATAA+TGG - chr2.4:32009651-32009670 MS.gene77160:intron 25.0%
!! TTTGGAAAGTTTGAAGTAAT+TGG + chr2.4:32011882-32011901 None:intergenic 25.0%
!! TTTGTCATAATGTTGTTTGT+TGG - chr2.4:32011062-32011081 MS.gene77160:intron 25.0%
!!! ATCATGTTGCCTTTTAAAAT+TGG - chr2.4:32012002-32012021 MS.gene77160:intron 25.0%
!!! TCATGTTGCCTTTTAAAATT+GGG - chr2.4:32012003-32012022 MS.gene77160:intron 25.0%
!!! TGATTGTTGTTTTATAAGTG+TGG + chr2.4:32010486-32010505 None:intergenic 25.0%
AATATCATACCAATCCTTCT+TGG + chr2.4:32009708-32009727 None:intergenic 30.0%
AGAAAATGTTGTCAAATAGC+GGG + chr2.4:32010317-32010336 None:intergenic 30.0%
CGAGAATAAAAATGAAGACA+TGG + chr2.4:32010662-32010681 None:intergenic 30.0%
CTACTGATAATTACACATTG+AGG - chr2.4:32011210-32011229 MS.gene77160:intron 30.0%
GAACATAAGTAACTAAGTGA+CGG + chr2.4:32010129-32010148 None:intergenic 30.0%
GTTACTTATGTTCGTAGATA+CGG - chr2.4:32010136-32010155 MS.gene77160:intron 30.0%
GTTGCTGAAATTTGATCAAT+TGG - chr2.4:32010826-32010845 MS.gene77160:intron 30.0%
GTTTGATCTTGGAAAATTGA+TGG - chr2.4:32011713-32011732 MS.gene77160:intron 30.0%
TAAGCTGAAAAAACATCTCA+TGG - chr2.4:32010068-32010087 MS.gene77160:intron 30.0%
! ATATGTCTTAGTCTTTCATG+GGG - chr2.4:32010804-32010823 MS.gene77160:CDS 30.0%
! CAATTTTCCAAGATCAAACT+TGG + chr2.4:32011712-32011731 None:intergenic 30.0%
! GAAGGATTGGTATGATATTA+AGG - chr2.4:32009709-32009728 MS.gene77160:intron 30.0%
! GTTTTCACACAAGCATTTAT+TGG + chr2.4:32011975-32011994 None:intergenic 30.0%
! GTTTTTCCTATAGGCTATTA+TGG - chr2.4:32010042-32010061 MS.gene77160:intron 30.0%
! TTATACCAAAAAGCATATCG+TGG - chr2.4:32010367-32010386 MS.gene77160:CDS 30.0%
!! AATGGATTTTGAAAAAGGGT+TGG + chr2.4:32009610-32009629 None:intergenic 30.0%
!! CTTTAAGGATCTTCACTTTT+CGG + chr2.4:32011686-32011705 None:intergenic 30.0%
!! GGTTTGATTTGTACATTGTT+AGG - chr2.4:32009838-32009857 MS.gene77160:intron 30.0%
!! GGTTTGATTTTGTTTCGATT+TGG - chr2.4:32012303-32012322 MS.gene77160:intron 30.0%
AAATTCATTCCCGAGATGAT+TGG - chr2.4:32011606-32011625 MS.gene77160:intron 35.0%
AACCATCTAAAAAGTGGCTT+TGG - chr2.4:32012282-32012301 MS.gene77160:intron 35.0%
AACTTCCATTCTCATGTAAG+TGG + chr2.4:32010708-32010727 None:intergenic 35.0%
AATTCATTCCCGAGATGATT+GGG - chr2.4:32011607-32011626 MS.gene77160:intron 35.0%
ACAATCTAGTCAGATTAGAC+AGG - chr2.4:32011292-32011311 MS.gene77160:intron 35.0%
AGATAGATATTTCGAGCAGA+GGG + chr2.4:32010444-32010463 None:intergenic 35.0%
ATCCTGTGATTTGAAGGAAT+TGG - chr2.4:32011578-32011597 MS.gene77160:intron 35.0%
ATTCTCATGTAAGTGGAAAG+AGG + chr2.4:32010701-32010720 None:intergenic 35.0%
ATTTCACAACTGACAAACTG+AGG - chr2.4:32011135-32011154 MS.gene77160:intron 35.0%
ATTTCTTAACATGTGCCCTA+AGG + chr2.4:32012045-32012064 None:intergenic 35.0%
CGAAATATCTATCTAGCACT+GGG - chr2.4:32010450-32010469 MS.gene77160:intron 35.0%
CTACGGATCTGAACAAATAA+AGG + chr2.4:32011525-32011544 None:intergenic 35.0%
CTTCTAAACTTGTGCCTTAA+GGG - chr2.4:32012167-32012186 MS.gene77160:intron 35.0%
CTTTCCAAAAGCTCTCTATT+CGG - chr2.4:32011893-32011912 MS.gene77160:intron 35.0%
GACAAGAAAAAAGACTGAAC+TGG - chr2.4:32010571-32010590 MS.gene77160:intron 35.0%
GTAGATCACATATCGCTATA+GGG + chr2.4:32010422-32010441 None:intergenic 35.0%
TAGATAGATATTTCGAGCAG+AGG + chr2.4:32010445-32010464 None:intergenic 35.0%
TCATGCTAAAAGAGAACAGA+AGG + chr2.4:32012233-32012252 None:intergenic 35.0%
TCCTTGTAACCAAAACCAAT+GGG + chr2.4:32010777-32010796 None:intergenic 35.0%
TCGAAATATCTATCTAGCAC+TGG - chr2.4:32010449-32010468 MS.gene77160:intron 35.0%
TCTTTCCACTTACATGAGAA+TGG - chr2.4:32010700-32010719 MS.gene77160:CDS 35.0%
TGAACTGGCATTATCATGAT+GGG - chr2.4:32010586-32010605 MS.gene77160:intron 35.0%
TGATCTTGGAAAATTGATGG+AGG - chr2.4:32011716-32011735 MS.gene77160:intron 35.0%
TTCCTTGTAACCAAAACCAA+TGG + chr2.4:32010778-32010797 None:intergenic 35.0%
TTTCTTAACATGTGCCCTAA+GGG + chr2.4:32012044-32012063 None:intergenic 35.0%
! ACATTGAGGATGTTTTGCAT+TGG - chr2.4:32011224-32011243 MS.gene77160:intron 35.0%
! CTTTATTTGTTCAGATCCGT+AGG - chr2.4:32011523-32011542 MS.gene77160:intron 35.0%
! TAGAGTGTTTGAGGTTTCAT+TGG - chr2.4:32010922-32010941 MS.gene77160:intron 35.0%
! TTGTTCCACGATATGCTTTT+TGG + chr2.4:32010375-32010394 None:intergenic 35.0%
! TTTCATTGGCTGATCTTCAA+GGG - chr2.4:32010936-32010955 MS.gene77160:CDS 35.0%
!! AATAATGTTGGTCTTGCTTG+TGG + chr2.4:32010546-32010565 None:intergenic 35.0%
!! AATGTTAACTTGTGCCCTTA+AGG + chr2.4:32012184-32012203 None:intergenic 35.0%
!! ATCCAACACATGTTTTAGGT+AGG - chr2.4:32011388-32011407 MS.gene77160:intron 35.0%
!! TTTCTAAAGTATCGAGTCCA+GGG - chr2.4:32010624-32010643 MS.gene77160:intron 35.0%
!!! AAATCACTGGTTTTGAACCT+AGG - chr2.4:32011448-32011467 MS.gene77160:intron 35.0%
!!! AACCAAAGCCACTTTTTAGA+TGG + chr2.4:32012287-32012306 None:intergenic 35.0%
!!! GCTATTTAACCCATTGGTTT+TGG - chr2.4:32010765-32010784 MS.gene77160:CDS 35.0%
!!! TTTTCTAAAGTATCGAGTCC+AGG - chr2.4:32010623-32010642 MS.gene77160:intron 35.0%
AAAGCTCCCAAGTTTGATCT+TGG - chr2.4:32011702-32011721 MS.gene77160:intron 40.0%
AACTGACAAACTGAGGTCAT+TGG - chr2.4:32011142-32011161 MS.gene77160:intron 40.0%
AAGAACAAGCGGATCTCTAA+GGG - chr2.4:32009553-32009572 MS.gene77160:CDS 40.0%
AATCTCCTTCCCAATCATCT+CGG + chr2.4:32011619-32011638 None:intergenic 40.0%
ACCATGCATTCAGGAAGATT+AGG - chr2.4:32010966-32010985 MS.gene77160:CDS 40.0%
ACCTAATCTTCCTGAATGCA+TGG + chr2.4:32010970-32010989 None:intergenic 40.0%
AGAAGACAAAGCATGATCCT+AGG + chr2.4:32011468-32011487 None:intergenic 40.0%
AGCATCATCCTGTGATTTGA+AGG - chr2.4:32011572-32011591 MS.gene77160:intron 40.0%
AGCTATCTAAACAATCAGGG+AGG + chr2.4:32009915-32009934 None:intergenic 40.0%
ATCCATTTGCCAAGAAGGAT+TGG - chr2.4:32009696-32009715 MS.gene77160:intron 40.0%
ATTTGGTCCTTGTAGGTTCA+TGG - chr2.4:32012320-32012339 MS.gene77160:intron 40.0%
CAAGCTCCAACAATTTCAGT+AGG + chr2.4:32012410-32012429 None:intergenic 40.0%
CACTAAGTATACTCAGTCCA+AGG + chr2.4:32010186-32010205 None:intergenic 40.0%
CTGAACTGGCATTATCATGA+TGG - chr2.4:32010585-32010604 MS.gene77160:intron 40.0%
CTGACTAGATTGTGCATAAC+AGG + chr2.4:32011285-32011304 None:intergenic 40.0%
CTTGTAGTGCAGAAAACAGT+GGG - chr2.4:32010248-32010267 MS.gene77160:CDS 40.0%
GAATGACAGATTGCTTCAGA+TGG - chr2.4:32010890-32010909 MS.gene77160:intron 40.0%
GACAGAGGAAAACAATACCT+TGG + chr2.4:32009795-32009814 None:intergenic 40.0%
GACCAATTCCTTCAAATCAC+AGG + chr2.4:32011583-32011602 None:intergenic 40.0%
GAGGAACCTACTGAAATTGT+TGG - chr2.4:32012401-32012420 MS.gene77160:CDS 40.0%
GATCAAACTTGGGAGCTTTA+AGG + chr2.4:32011701-32011720 None:intergenic 40.0%
GCTATCTAAACAATCAGGGA+GGG + chr2.4:32009914-32009933 None:intergenic 40.0%
GCTTCTAAACTTGTGCCTTA+AGG - chr2.4:32012166-32012185 MS.gene77160:intron 40.0%
GGTAGATCACATATCGCTAT+AGG + chr2.4:32010423-32010442 None:intergenic 40.0%
GTAATCTCCATGAACCTACA+AGG + chr2.4:32012330-32012349 None:intergenic 40.0%
GTCCTACCTAAAACATGTGT+TGG + chr2.4:32011393-32011412 None:intergenic 40.0%
TAAGAACAAGCGGATCTCTA+AGG - chr2.4:32009552-32009571 MS.gene77160:CDS 40.0%
TACCAATCCTTCTTGGCAAA+TGG + chr2.4:32009701-32009720 None:intergenic 40.0%
TCAAATCACAGGATGATGCT+TGG + chr2.4:32011572-32011591 None:intergenic 40.0%
TCATCTCGGGAATGAATTTG+CGG + chr2.4:32011605-32011624 None:intergenic 40.0%
TCTTGTAGTGCAGAAAACAG+TGG - chr2.4:32010247-32010266 MS.gene77160:CDS 40.0%
TGAGAGCTGAAGATGTTCAA+GGG - chr2.4:32010990-32011009 MS.gene77160:CDS 40.0%
TGATCTCAACCATCTTCCTA+CGG + chr2.4:32011542-32011561 None:intergenic 40.0%
TTCTTCACCTGGAAAACTGA+AGG + chr2.4:32009737-32009756 None:intergenic 40.0%
TTGAGAGCTGAAGATGTTCA+AGG - chr2.4:32010989-32011008 MS.gene77160:CDS 40.0%
! ACTCAAGCATAGAGTGTTTG+AGG - chr2.4:32010913-32010932 MS.gene77160:intron 40.0%
! AGTAGTCTTGACATCCACAT+GGG + chr2.4:32011195-32011214 None:intergenic 40.0%
! CGGTATCCAACACATGTTTT+AGG - chr2.4:32011384-32011403 MS.gene77160:intron 40.0%
! CTAGCTGCTATTTAACCCAT+TGG - chr2.4:32010759-32010778 MS.gene77160:CDS 40.0%
! GAAACCGAATAGAGAGCTTT+TGG + chr2.4:32011900-32011919 None:intergenic 40.0%
! GTTTCATTGGCTGATCTTCA+AGG - chr2.4:32010935-32010954 MS.gene77160:CDS 40.0%
! GTTTTCCAGGTGAAGAATGT+CGG - chr2.4:32009740-32009759 MS.gene77160:intron 40.0%
! TTCATTGGCTGATCTTCAAG+GGG - chr2.4:32010937-32010956 MS.gene77160:CDS 40.0%
!! AGTGTTAACTTGTGCCCTTA+GGG - chr2.4:32012027-32012046 MS.gene77160:intron 40.0%
!! CAATGGGTTAAATAGCAGCT+AGG + chr2.4:32010761-32010780 None:intergenic 40.0%
!! TAGTGTTAACTTGTGCCCTT+AGG - chr2.4:32012026-32012045 MS.gene77160:intron 40.0%
!! TGTTTCGATTTGGTCCTTGT+AGG - chr2.4:32012313-32012332 MS.gene77160:intron 40.0%
!! TTCTAAAGTATCGAGTCCAG+GGG - chr2.4:32010625-32010644 MS.gene77160:intron 40.0%
!! TTGGTTGTTACTTTGGTCAC+TGG - chr2.4:32011820-32011839 MS.gene77160:intron 40.0%
!! TTGTCAAATAGCGGGTAAAG+TGG + chr2.4:32010309-32010328 None:intergenic 40.0%
!! TTTGCATTGGCTTTACCAAG+AGG - chr2.4:32011237-32011256 MS.gene77160:intron 40.0%
!!! ACCCATTGGTTTTGGTTACA+AGG - chr2.4:32010773-32010792 MS.gene77160:CDS 40.0%
AACTCTTGTTGAAGCCCATG+TGG - chr2.4:32011178-32011197 MS.gene77160:intron 45.0%
ACATGTGTTGGATACCGTGT+TGG + chr2.4:32011381-32011400 None:intergenic 45.0%
ACTCAGTCCAAGGACATGTT+TGG + chr2.4:32010176-32010195 None:intergenic 45.0%
AGAACAAGCGGATCTCTAAG+GGG - chr2.4:32009554-32009573 MS.gene77160:CDS 45.0%
ATCTCCTTCCCAATCATCTC+GGG + chr2.4:32011618-32011637 None:intergenic 45.0%
ATGTTGGCACAAAGGTAGAG+AGG - chr2.4:32012357-32012376 MS.gene77160:intron 45.0%
CAAGGGGTAAATACTAGACG+TGG + chr2.4:32011649-32011668 None:intergenic 45.0%
CGGATGAACACATTCTGCAA+GGG + chr2.4:32011666-32011685 None:intergenic 45.0%
GATCCGCTTGTTCTTACCAA+CGG + chr2.4:32009548-32009567 None:intergenic 45.0%
GGATGAACACATTCTGCAAG+GGG + chr2.4:32011665-32011684 None:intergenic 45.0%
GGTTCATGGAGATTACTCTG+AGG - chr2.4:32012334-32012353 MS.gene77160:intron 45.0%
GTCCAAGGACATGTTTGGAT+TGG + chr2.4:32010171-32010190 None:intergenic 45.0%
TCGGATGAACACATTCTGCA+AGG + chr2.4:32011667-32011686 None:intergenic 45.0%
TGCCAATCCAAACATGTCCT+TGG - chr2.4:32010166-32010185 MS.gene77160:intron 45.0%
TGCTGATCCATTTGCCAAGA+AGG - chr2.4:32009691-32009710 MS.gene77160:intron 45.0%
TGTTCAGATCCGTAGGAAGA+TGG - chr2.4:32011530-32011549 MS.gene77160:intron 45.0%
! ATCTCTAAGGGGAAGAAAGG+TGG - chr2.4:32009565-32009584 MS.gene77160:CDS 45.0%
! CAGTAGTCTTGACATCCACA+TGG + chr2.4:32011196-32011215 None:intergenic 45.0%
! GGAGATTACTCTGAGGATGT+TGG - chr2.4:32012341-32012360 MS.gene77160:intron 45.0%
! TAAGGCTCCTTCAGTTTTCC+AGG - chr2.4:32009727-32009746 MS.gene77160:intron 45.0%
!! ATATTATGAAAATAAATATT+GGG + chr2.4:32011784-32011803 None:intergenic 5.0%
!!! AAACAAAATTAAAATTTTAA+AGG + chr2.4:32012209-32012228 None:intergenic 5.0%
!!! AACAAAATTAAAATTTTAAA+GGG + chr2.4:32012208-32012227 None:intergenic 5.0%
AGAGAGGCCTGCTGATGAAA+TGG - chr2.4:32012373-32012392 MS.gene77160:CDS 50.0%
CATAACAGGTCCTCTTCACC+TGG + chr2.4:32011271-32011290 None:intergenic 50.0%
CATTCCCGAGATGATTGGGA+AGG - chr2.4:32011611-32011630 MS.gene77160:intron 50.0%
GCCTGCTGATGAAATGGTGA+CGG - chr2.4:32012379-32012398 MS.gene77160:CDS 50.0%
TACCAAGAGGAGAGCTAACC+AGG - chr2.4:32011250-32011269 MS.gene77160:intron 50.0%
TCCGTCACCATTTCATCAGC+AGG + chr2.4:32012383-32012402 None:intergenic 50.0%
TCTTGCCGACATTCTTCACC+TGG + chr2.4:32009748-32009767 None:intergenic 50.0%
TGAGGTCATTGGTGCGTAAG+TGG - chr2.4:32011153-32011172 MS.gene77160:intron 50.0%
! CGGATCTCTAAGGGGAAGAA+AGG - chr2.4:32009562-32009581 MS.gene77160:CDS 50.0%
! CTCTGAGGATGTTGGCACAA+AGG - chr2.4:32012349-32012368 MS.gene77160:intron 50.0%
! TGGCCGTTGGTAAGAACAAG+CGG - chr2.4:32009542-32009561 MS.gene77160:CDS 50.0%
!! ATGTTGGTCTTGCTTGTGGC+AGG + chr2.4:32010542-32010561 None:intergenic 50.0%
!! TGCTGATGAAATGGTGACGG+AGG - chr2.4:32012382-32012401 MS.gene77160:CDS 50.0%
!! TGTTGGTCTTGCTTGTGGCA+GGG + chr2.4:32010541-32010560 None:intergenic 50.0%
ACCCTCGTGTCTCGTACTCA+GGG - chr2.4:32009767-32009786 MS.gene77160:intron 55.0%
ACCCTGAGTACGAGACACGA+GGG + chr2.4:32009771-32009790 None:intergenic 55.0%
CATGGGAATGTCATGTCCCC+TGG + chr2.4:32010644-32010663 None:intergenic 55.0%
GGAGAGCTAACCAGGTGAAG+AGG - chr2.4:32011258-32011277 MS.gene77160:intron 55.0%
GGGACGAAGACCATGCATTC+AGG - chr2.4:32010957-32010976 MS.gene77160:CDS 55.0%
TACCCTGAGTACGAGACACG+AGG + chr2.4:32009772-32009791 None:intergenic 55.0%
! CACCTGGTTAGCTCTCCTCT+TGG + chr2.4:32011255-32011274 None:intergenic 55.0%
! GTCTCGTACTCAGGGTACCA+AGG - chr2.4:32009775-32009794 MS.gene77160:intron 55.0%
CTTGTGGCAGGGCAGAAGTG+GGG + chr2.4:32010530-32010549 None:intergenic 60.0%
GACCCTCGTGTCTCGTACTC+AGG - chr2.4:32009766-32009785 MS.gene77160:intron 60.0%
GCTTGTGGCAGGGCAGAAGT+GGG + chr2.4:32010531-32010550 None:intergenic 60.0%
TGCTTGTGGCAGGGCAGAAG+TGG + chr2.4:32010532-32010551 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 32009541 32012430 32009541 ID=MS.gene77160
chr2.4 mRNA 32009541 32012430 32009541 ID=MS.gene77160.t1;Parent=MS.gene77160
chr2.4 exon 32012372 32012430 32012372 ID=MS.gene77160.t1.exon1;Parent=MS.gene77160.t1
chr2.4 CDS 32012372 32012430 32012372 ID=cds.MS.gene77160.t1;Parent=MS.gene77160.t1
chr2.4 exon 32012175 32012280 32012175 ID=MS.gene77160.t1.exon2;Parent=MS.gene77160.t1
chr2.4 CDS 32012175 32012280 32012175 ID=cds.MS.gene77160.t1;Parent=MS.gene77160.t1
chr2.4 exon 32010935 32011072 32010935 ID=MS.gene77160.t1.exon3;Parent=MS.gene77160.t1
chr2.4 CDS 32010935 32011072 32010935 ID=cds.MS.gene77160.t1;Parent=MS.gene77160.t1
chr2.4 exon 32010658 32010843 32010658 ID=MS.gene77160.t1.exon4;Parent=MS.gene77160.t1
chr2.4 CDS 32010658 32010843 32010658 ID=cds.MS.gene77160.t1;Parent=MS.gene77160.t1
chr2.4 exon 32010234 32010434 32010234 ID=MS.gene77160.t1.exon5;Parent=MS.gene77160.t1
chr2.4 CDS 32010234 32010434 32010234 ID=cds.MS.gene77160.t1;Parent=MS.gene77160.t1
chr2.4 exon 32009541 32009636 32009541 ID=MS.gene77160.t1.exon6;Parent=MS.gene77160.t1
chr2.4 CDS 32009541 32009636 32009541 ID=cds.MS.gene77160.t1;Parent=MS.gene77160.t1
Gene Sequence

>MS.gene77160

ATGGCCGTTGGTAAGAACAAGCGGATCTCTAAGGGGAAGAAAGGTGGCAAGAAGAAAGCGTTAGTACCAACCCTTTTTCAAAATCCATTCAAATTTTCATTTTTTACAATTTTCCTTGCATTTTCTATAATGGTTTCTATGAACTTGCAGTGCTGATCCATTTGCCAAGAAGGATTGGTATGATATTAAGGCTCCTTCAGTTTTCCAGGTGAAGAATGTCGGCAAGACCCTCGTGTCTCGTACTCAGGGTACCAAGGTATTGTTTTCCTCTGTCATGTTTTTTGTTTTTTGTAATTTGGTTTGATTTGTACATTGTTAGGTTATTGTACGAATTTATATGATCCTATCTTACTTTCATTGAATATCTGGACCCTCCCTGATTGTTTAGATAGCTTAATTCAGAGCTTTTATATTCAAGTGTTGTTATATAAGTGTTTATGTATAAACTATTTTTATAACTAAAGATAGAATAAAGTCAAATTGTTTTCACATAAGCTAAAAGTTTTTCCTATAGGCTATTATGGAAATAAGCTGAAAAAACATCTCATGGATATGTCATAAGCTTTTTCGTTAAGCTCTTTCAAACCGTCACTTAGTTACTTATGTTCGTAGATACGGTCAAATATGCCAATCCAAACATGTCCTTGGACTGAGTATACTTAGTGACATTATAGTGTTCGTGTTGTGTTTTTGTCAGTGTTCTCAATCTTGTAGTGCAGAAAACAGTGGGTTGTTAAAATTCTGCTACACAATAGTGCTATAGTGCCACTTTACCCGCTATTTGACAACATTTTCTTTTGCTAAATAGTGTACCATAAAACAATTTTTATACCAAAAAGCATATCGTGGAACAATAGTGATTTGTATAAATTCAGCTACCCTATAGCGATATGTGATCTACCCTCTGCTCGAAATATCTATCTAGCACTGGGAGAGATGCGTCCACACTTATAAAACAACAATCATATCTTAAACTATCAGCTTGACCCCACTTCTGCCCTGCCACAAGCAAGACCAACATTATTTAAAAGACAAGAAAAAAGACTGAACTGGCATTATCATGATGGGCTCAAACTTAAATATTTTCTAAAGTATCGAGTCCAGGGGACATGACATTCCCATGTCTTCATTTTTATTCTCGTGATTAGAGTGAGTGTCCTCTTTCCACTTACATGAGAATGGAAGTTTTAATGTTACATTGTAACATATATTCATTTCCTAGCTGCTATTTAACCCATTGGTTTTGGTTACAAGGAATAACAAATATGTCTTAGTCTTTCATGGGGTTGCTGAAATTTGATCAATTGGAGTTTTGCACGCTTAATGCTTATTTAGAAAATGGTTGTTTTGAATGACAGATTGCTTCAGATGGACTCAAGCATAGAGTGTTTGAGGTTTCATTGGCTGATCTTCAAGGGGACGAAGACCATGCATTCAGGAAGATTAGGTTGAGAGCTGAAGATGTTCAAGGGAAAAATCTTTTGACAAATTTCTGGGTATGATAAGCTGCCGAACCATCTATTTGTCATAATGTTGTTTGTTGGTATTTTTTTTTAATTTGATTTTGATTTTTTTTTTTATTTACAGGGGATGAATTTCACAACTGACAAACTGAGGTCATTGGTGCGTAAGTGGCAAACTCTTGTTGAAGCCCATGTGGATGTCAAGACTACTGATAATTACACATTGAGGATGTTTTGCATTGGCTTTACCAAGAGGAGAGCTAACCAGGTGAAGAGGACCTGTTATGCACAATCTAGTCAGATTAGACAGGTAGATACATTGACGCTTTTCTTTTCAAATCATACTAGTTTTCTAGATTTATATATTAGTGTATCCAACACGGTATCCAACACATGTTTTAGGTAGGACCTTTCTTTTATGAAATGCTGTTATATATAAAGAAGAAATCACTGGTTTTGAACCTAGGATCATGCTTTGTCTTCTTCTAAGATATTTCTTATTAGTTACTAATGTTACACCTTTATTTGTTCAGATCCGTAGGAAGATGGTTGAGATCATGATCAACCAAGCATCATCCTGTGATTTGAAGGAATTGGTCCGCAAATTCATTCCCGAGATGATTGGGAAGGAGATTGAGAAAGCCACGTCTAGTATTTACCCCTTGCAGAATGTGTTCATCCGAAAAGTGAAGATCCTTAAAGCTCCCAAGTTTGATCTTGGAAAATTGATGGAGGTATTTTTTTGAAACAACTTTTTTATGTTTGTTCTCATTCACACCCAATATTTATTTTCATAATATCACCTGAATAAAAACTTTGGTTGTTACTTTGGTCACTGGTGTTATTTGCTTGACGAGATGAACTCTTTAATATAACCAATTACTTCAAACTTTCCAAAAGCTCTCTATTCGGTTTCTCCATAAACAACCTTCAGATTATTACAAGTTCAAAACTAAATACTTACTGATCCAATAAATGCTTGTGTGAAAACAAACTTAATCATGTTGCCTTTTAAAATTGGGTAGTGTTAACTTGTGCCCTTAGGGCACATGTTAAGAAATCCAAAAAAAGAAATATTCTCTTAAACTTTGTGCATTCAATTCATTAAAACATTAAAAAGTAAATTTAATGCACGCATTTCAATACATTATTTCTAGTTTTGGCTTCTAAACTTGTGCCTTAAGGGCACAAGTTAACATTTCCCTTTAAAATTTTAATTTTGTTTTCCTTCTGTTCTCTTTTAGCATGAGTATGCAGTTGTCACGTGTGCACACTTATAACCATCTAAAAAGTGGCTTTGGTTTGATTTTGTTTCGATTTGGTCCTTGTAGGTTCATGGAGATTACTCTGAGGATGTTGGCACAAAGGTAGAGAGGCCTGCTGATGAAATGGTGACGGAGGAACCTACTGAAATTGTTGGAGCTTGA
Protein sequence

>MS.gene77160.t1

MAVGKNKRISKGKKGGKKKAADPFAKKDWYDIKAPSVFQVKNVGKTLVSRTQGTKIASDGLKHRVFEVSLADLQGDEDHAFRKIRLRAEDVQGKNLLTNFWGMNFTTDKLRSLVRKWQTLVEAHVDVKTTDNYTLRMFCIGFTKRRANQVKRTCYAQSSQIRQIRRKMVEIMINQASSCDLKELVRKFIPEMIGKEIEKATSSIYPLQNVFIRKVKILKAPKFDLGKLMEVHGDYSEDVGTKVERPADEMVTEEPTEIVGA