AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene043818

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043818.t1 MTR_1g103100 97.701 261 6 0 1 261 1 261 0.0 530
MS.gene043818.t1 MTR_3g012040 95.785 261 11 0 1 261 1 261 0.0 497
MS.gene043818.t1 MTR_5g006440 94.636 261 14 0 1 261 1 261 9.71e-179 492
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043818.t1 AT3G04840 85.115 262 38 1 1 261 1 262 1.68e-163 453
MS.gene043818.t1 AT4G34670 85.496 262 37 1 1 261 1 262 4.36e-163 452

Find 78 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCAAACTTGGGAGCTTTA+AGG 0.168578 2.2:+27808659 None:intergenic
AATATCATACCAATCCTTCT+TGG 0.295389 2.2:+27810583 None:intergenic
ATTTGGTCCTTGTAGGTTCA+TGG 0.297913 2.2:-27808231 MS.gene043818:intron
GAAGGATTGGTATGATATTA+AGG 0.312711 2.2:-27810579 MS.gene043818:CDS
CTGACTAGATTGTGCATAAC+AGG 0.339955 2.2:+27809075 None:intergenic
AAAGCTCCCAAGTTTGATCT+TGG 0.357514 2.2:-27808655 MS.gene043818:CDS
TAAGAACAAGCGGATCTCTA+AGG 0.365175 2.2:-27810736 MS.gene043818:CDS
GTTTGATCTTGGAAAATTGA+TGG 0.369245 2.2:-27808644 MS.gene043818:CDS
AAATTCATTCCCGAGATGAT+TGG 0.381702 2.2:-27808751 MS.gene043818:CDS
GGGACGAAGACCATGCATTC+AGG 0.393353 2.2:-27809402 MS.gene043818:CDS
ATCCTGTGATTTGAAGGAAT+TGG 0.402781 2.2:-27808779 MS.gene043818:CDS
GACCCTCGTGTCTCGTACTC+AGG 0.406163 2.2:-27810522 MS.gene043818:CDS
TACCAATCCTTCTTGGCAAA+TGG 0.412105 2.2:+27810590 None:intergenic
TAGAGTGTTTGAGGTTTCAT+TGG 0.413434 2.2:-27809437 MS.gene043818:CDS
GTTTCATTGGCTGATCTTCA+AGG 0.434675 2.2:-27809424 MS.gene043818:CDS
TGCTGATCCATTTGCCAAGA+AGG 0.436339 2.2:-27810597 MS.gene043818:CDS
AATTCATTCCCGAGATGATT+GGG 0.437177 2.2:-27808750 MS.gene043818:CDS
AGCATCATCCTGTGATTTGA+AGG 0.439297 2.2:-27808785 MS.gene043818:CDS
TACCAAGAGGAGAGCTAACC+AGG 0.440122 2.2:-27809107 MS.gene043818:CDS
TCCGTCACCATTTCATCAGC+AGG 0.444686 2.2:+27808171 None:intergenic
ACTCAAGCATAGAGTGTTTG+AGG 0.454600 2.2:-27809446 MS.gene043818:CDS
ACCATGCATTCAGGAAGATT+AGG 0.462005 2.2:-27809393 MS.gene043818:CDS
ATCCATTTGCCAAGAAGGAT+TGG 0.465718 2.2:-27810592 MS.gene043818:CDS
ATCTCCTTCCCAATCATCTC+GGG 0.483111 2.2:+27808742 None:intergenic
GAGGAACCTACTGAAATTGT+TGG 0.484047 2.2:-27808150 MS.gene043818:CDS
TGTTCAGATCCGTAGGAAGA+TGG 0.490433 2.2:-27808827 MS.gene043818:intron
CACCTGGTTAGCTCTCCTCT+TGG 0.502596 2.2:+27809105 None:intergenic
AACTGACAAACTGAGGTCAT+TGG 0.506332 2.2:-27809215 MS.gene043818:CDS
TGAGGTCATTGGTGCGTAAG+TGG 0.507827 2.2:-27809204 MS.gene043818:CDS
CGGATCTCTAAGGGGAAGAA+AGG 0.516719 2.2:-27810726 MS.gene043818:CDS
AAGAACAAGCGGATCTCTAA+GGG 0.521243 2.2:-27810735 MS.gene043818:CDS
CTTGTTCTTACCAACGGCCA+TGG 0.523346 2.2:+27810749 None:intergenic
ACCTAATCTTCCTGAATGCA+TGG 0.523822 2.2:+27809392 None:intergenic
TTTCATTGGCTGATCTTCAA+GGG 0.523908 2.2:-27809423 MS.gene043818:CDS
TCATCTCGGGAATGAATTTG+CGG 0.528925 2.2:+27808755 None:intergenic
AGAGAGGCCTGCTGATGAAA+TGG 0.532099 2.2:-27808178 MS.gene043818:CDS
TCTTGCCGACATTCTTCACC+TGG 0.534041 2.2:+27810543 None:intergenic
AATCTCCTTCCCAATCATCT+CGG 0.535678 2.2:+27808741 None:intergenic
TTGAGAGCTGAAGATGTTCA+AGG 0.537527 2.2:-27809370 MS.gene043818:CDS
CGGATGAACACATTCTGCAA+GGG 0.539130 2.2:+27808694 None:intergenic
TCAAATCACAGGATGATGCT+TGG 0.544201 2.2:+27808788 None:intergenic
CATAACAGGTCCTCTTCACC+TGG 0.545447 2.2:+27809089 None:intergenic
GGAGAGCTAACCAGGTGAAG+AGG 0.557404 2.2:-27809099 MS.gene043818:CDS
GAATGACAGATTGCTTCAGA+TGG 0.560187 2.2:-27809469 MS.gene043818:intron
CTCTGAGGATGTTGGCACAA+AGG 0.562498 2.2:-27808202 MS.gene043818:CDS
CTTTATTTGTTCAGATCCGT+AGG 0.563835 2.2:-27808834 MS.gene043818:intron
GATCCGCTTGTTCTTACCAA+CGG 0.569816 2.2:+27810743 None:intergenic
GACCAATTCCTTCAAATCAC+AGG 0.574407 2.2:+27808777 None:intergenic
TCGGATGAACACATTCTGCA+AGG 0.575354 2.2:+27808693 None:intergenic
ACAATCTAGTCAGATTAGAC+AGG 0.580594 2.2:-27809065 MS.gene043818:intron
CAAGCTCCAACAATTTCAGT+AGG 0.582102 2.2:+27808144 None:intergenic
GGAGATTACTCTGAGGATGT+TGG 0.587473 2.2:-27808210 MS.gene043818:CDS
TTCTTCACCTGGAAAACTGA+AGG 0.588083 2.2:+27810554 None:intergenic
TGATCTCAACCATCTTCCTA+CGG 0.590794 2.2:+27808818 None:intergenic
TTCATTGGCTGATCTTCAAG+GGG 0.595752 2.2:-27809422 MS.gene043818:CDS
CAGTAGTCTTGACATCCACA+TGG 0.596992 2.2:+27809164 None:intergenic
AACAGTGAAACCATGGCCGT+TGG 0.599680 2.2:-27810759 None:intergenic
CATTCCCGAGATGATTGGGA+AGG 0.605231 2.2:-27808746 MS.gene043818:CDS
TGATCTTGGAAAATTGATGG+AGG 0.612140 2.2:-27808641 MS.gene043818:intron
TGAGAGCTGAAGATGTTCAA+GGG 0.612312 2.2:-27809369 MS.gene043818:CDS
ACCCTCGTGTCTCGTACTCA+GGG 0.615233 2.2:-27810521 MS.gene043818:CDS
ATCTCTAAGGGGAAGAAAGG+TGG 0.621403 2.2:-27810723 MS.gene043818:CDS
AACTCTTGTTGAAGCCCATG+TGG 0.622205 2.2:-27809179 MS.gene043818:CDS
GTCTCGTACTCAGGGTACCA+AGG 0.623684 2.2:-27810513 MS.gene043818:intron
TGGCCGTTGGTAAGAACAAG+CGG 0.624911 2.2:-27810746 MS.gene043818:CDS
GCCTGCTGATGAAATGGTGA+CGG 0.629382 2.2:-27808172 MS.gene043818:CDS
GGATGAACACATTCTGCAAG+GGG 0.631103 2.2:+27808695 None:intergenic
CAAGGGGTAAATACTAGACG+TGG 0.633754 2.2:+27808711 None:intergenic
CTACTGATAATTACACATTG+AGG 0.636083 2.2:-27809147 MS.gene043818:CDS
TTTGCATTGGCTTTACCAAG+AGG 0.642280 2.2:-27809120 MS.gene043818:CDS
TACCCTGAGTACGAGACACG+AGG 0.648373 2.2:+27810519 None:intergenic
ATGTTGGCACAAAGGTAGAG+AGG 0.654238 2.2:-27808194 MS.gene043818:CDS
ATTTCACAACTGACAAACTG+AGG 0.681977 2.2:-27809222 MS.gene043818:CDS
TGCTGATGAAATGGTGACGG+AGG 0.686062 2.2:-27808169 MS.gene043818:CDS
ACCCTGAGTACGAGACACGA+GGG 0.700568 2.2:+27810520 None:intergenic
AGTAGTCTTGACATCCACAT+GGG 0.706350 2.2:+27809165 None:intergenic
AGAACAAGCGGATCTCTAAG+GGG 0.721950 2.2:-27810734 MS.gene043818:CDS
GGTTCATGGAGATTACTCTG+AGG 0.741731 2.2:-27808217 MS.gene043818:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAAAATTAAAATTTTAAA+AGG + chr2.2:27810547-27810566 None:intergenic 0.0%
!! GATATTATGAAAATAAATAT+TGG + chr2.2:27810318-27810337 None:intergenic 10.0%
!!! GATTTTTTTATTTTATTTAC+AGG - chr2.2:27809639-27809658 MS.gene043818:intron 10.0%
!!! TTAGTATAAAAATTGTTTTA+TGG + chr2.2:27808887-27808906 None:intergenic 10.0%
!!! TTTTTTTATTTTATTTACAG+GGG - chr2.2:27809641-27809660 MS.gene043818:intron 10.0%
!! GTAAAAAATGAAAATTTGAA+TGG + chr2.2:27808230-27808249 None:intergenic 15.0%
!! AAAAAAATGTTGTCAAATAG+CGG + chr2.2:27808850-27808869 None:intergenic 20.0%
!! ATATTCAATGAAAGTAAGAT+AGG + chr2.2:27808481-27808500 None:intergenic 20.0%
!! CTTAATGCTTATTTAGAAAA+TGG - chr2.2:27809391-27809410 MS.gene043818:CDS 20.0%
!!! AAAATCTTTTGACAAATTTC+TGG - chr2.2:27809545-27809564 MS.gene043818:intron 20.0%
!!! AAATCTTTTGACAAATTTCT+GGG - chr2.2:27809546-27809565 MS.gene043818:intron 20.0%
!!! ATTTGAATGGATTTTGAAAA+AGG + chr2.2:27808217-27808236 None:intergenic 20.0%
!!! TCTTGTCTTTTAAATAATGT+TGG + chr2.2:27809089-27809108 None:intergenic 20.0%
!!! TTTGAATGGATTTTGAAAAA+GGG + chr2.2:27808216-27808235 None:intergenic 20.0%
! AAAAAATGTTGTCAAATAGC+GGG + chr2.2:27808849-27808868 None:intergenic 25.0%
! AAACCATTATAGAAAATGCA+AGG + chr2.2:27808259-27808278 None:intergenic 25.0%
! AACAGCATTTCATAAAAGAA+AGG + chr2.2:27809948-27809967 None:intergenic 25.0%
! AATAATCTGAAGGTTGTTTA+TGG + chr2.2:27810457-27810476 None:intergenic 25.0%
! AATTACAAAAAACATGACAG+AGG + chr2.2:27808412-27808431 None:intergenic 25.0%
! ACAACATTATGACAAATAGA+TGG + chr2.2:27809589-27809608 None:intergenic 25.0%
! ACTTATAACCATCTAAAAAG+TGG - chr2.2:27810615-27810634 MS.gene043818:intron 25.0%
! ACTTTCATTGAATATCTATC+TGG - chr2.2:27808486-27808505 MS.gene043818:intron 25.0%
! ATATATCAAGAAGAAATCAC+TGG - chr2.2:27809968-27809987 MS.gene043818:intron 25.0%
! ATATATTAGTGTATCCAACA+CGG - chr2.2:27809897-27809916 MS.gene043818:intron 25.0%
! ATTAAGCTATCTAAACAATC+AGG + chr2.2:27808518-27808537 None:intergenic 25.0%
! ATTATGACAAATAGATGGTT+CGG + chr2.2:27809584-27809603 None:intergenic 25.0%
! GAGAATAAAAATGAAGACAT+GGG + chr2.2:27809192-27809211 None:intergenic 25.0%
! TATCACCTGAATAAAAACTT+TGG - chr2.2:27810334-27810353 MS.gene043818:intron 25.0%
! TTAAGCTATCTAAACAATCA+GGG + chr2.2:27808517-27808536 None:intergenic 25.0%
! TTATTTCCATAATAGCCTAT+AGG + chr2.2:27808650-27808669 None:intergenic 25.0%
! TTGAACTTGTAATAATCTGA+AGG + chr2.2:27810467-27810486 None:intergenic 25.0%
!! AAAAACTTTGGTTGTTACTT+TGG - chr2.2:27810346-27810365 MS.gene043818:intron 25.0%
!! AAATATGTCTTAGTCTTTCA+TGG - chr2.2:27809333-27809352 MS.gene043818:intron 25.0%
!! AACAACCAAAGTTTTTATTC+AGG + chr2.2:27810342-27810361 None:intergenic 25.0%
!! AAGCTAAAAGTTTTTCCTAT+AGG - chr2.2:27808632-27808651 MS.gene043818:intron 25.0%
!! AATATGTCTTAGTCTTTCAT+GGG - chr2.2:27809334-27809353 MS.gene043818:intron 25.0%
!! AATTTTCCAAGATCAAACTT+GGG + chr2.2:27810244-27810263 None:intergenic 25.0%
!! TTATACTAAAAAGCATATCG+TGG - chr2.2:27808898-27808917 MS.gene043818:intron 25.0%
!! TTTCCTTGCATTTTCTATAA+TGG - chr2.2:27808253-27808272 MS.gene043818:intron 25.0%
!! TTTGGAAAGTTTGAAGTAAT+TGG + chr2.2:27810415-27810434 None:intergenic 25.0%
!! TTTGTCATAATGTTGTTTGT+TGG - chr2.2:27809593-27809612 MS.gene043818:intron 25.0%
!!! CTGTCATGTTTTTTGTAATT+TGG - chr2.2:27808412-27808431 MS.gene043818:intron 25.0%
!!! GGTTTGATTTTGTTTCTATT+TGG - chr2.2:27810642-27810661 MS.gene043818:intron 25.0%
!!! TGATTGTTGTTTTATAAGTG+TGG + chr2.2:27809017-27809036 None:intergenic 25.0%
AATATCATACCAATCCTTCT+TGG + chr2.2:27808310-27808329 None:intergenic 30.0%
ACTATAATGTCACTAAGTGA+CGG + chr2.2:27808728-27808747 None:intergenic 30.0%
CGAGAATAAAAATGAAGACA+TGG + chr2.2:27809193-27809212 None:intergenic 30.0%
CTACTGATAATTACACATTG+AGG - chr2.2:27809743-27809762 MS.gene043818:intron 30.0%
GTTGCTGAAATTTGATCAAT+TGG - chr2.2:27809357-27809376 MS.gene043818:CDS 30.0%
GTTTGATCTTGGAAAATTGA+TGG - chr2.2:27810246-27810265 MS.gene043818:intron 30.0%
TAAGCTGAAAAAACATCTCA+TGG - chr2.2:27808667-27808686 MS.gene043818:CDS 30.0%
! ATATGTCTTAGTCTTTCATG+GGG - chr2.2:27809335-27809354 MS.gene043818:intron 30.0%
! CAATTTTCCAAGATCAAACT+TGG + chr2.2:27810245-27810264 None:intergenic 30.0%
! GAAGGATTGGTATGATATTA+AGG - chr2.2:27808311-27808330 MS.gene043818:intron 30.0%
! GTTTTCACACAAGCATTTAT+TGG + chr2.2:27810508-27810527 None:intergenic 30.0%
! GTTTTTCCTATAGGCTATTA+TGG - chr2.2:27808641-27808660 MS.gene043818:intron 30.0%
!! AATGGATTTTGAAAAAGGGT+TGG + chr2.2:27808212-27808231 None:intergenic 30.0%
!! CTTTAAGGATCTTCACTTTT+CGG + chr2.2:27810219-27810238 None:intergenic 30.0%
!! GGTTTGATTTGTACATTGTT+AGG - chr2.2:27808433-27808452 MS.gene043818:intron 30.0%
!! TGTAGCAGAATTTTAACAAC+CGG + chr2.2:27808802-27808821 None:intergenic 30.0%
AAATTCATTCCCGAGATGAT+TGG - chr2.2:27810139-27810158 MS.gene043818:intron 35.0%
AACCATCTAAAAAGTGGCTT+TGG - chr2.2:27810621-27810640 MS.gene043818:intron 35.0%
AACTTCCATTCTCATGTAAG+TGG + chr2.2:27809239-27809258 None:intergenic 35.0%
AATTCATTCCCGAGATGATT+GGG - chr2.2:27810140-27810159 MS.gene043818:intron 35.0%
ACAATCTAGTCAGATTAGAC+AGG - chr2.2:27809825-27809844 MS.gene043818:intron 35.0%
AGATAGATATTTCGAGCAGA+GGG + chr2.2:27808975-27808994 None:intergenic 35.0%
ATCCTGTGATTTGAAGGAAT+TGG - chr2.2:27810111-27810130 MS.gene043818:intron 35.0%
ATTCTCATGTAAGTGGAAAG+AGG + chr2.2:27809232-27809251 None:intergenic 35.0%
ATTTCACAACTGACAAACTG+AGG - chr2.2:27809668-27809687 MS.gene043818:intron 35.0%
CGAAATATCTATCTAGCACT+GGG - chr2.2:27808981-27809000 MS.gene043818:intron 35.0%
CTACGGATCTGAACAAATAA+AGG + chr2.2:27810058-27810077 None:intergenic 35.0%
CTTTCCAAAAGCTCTCTATT+CGG - chr2.2:27810426-27810445 MS.gene043818:intron 35.0%
GACAAGAAAAAAGACTGAAC+TGG - chr2.2:27809102-27809121 MS.gene043818:CDS 35.0%
GTAGATCACATATCGCTATA+GGG + chr2.2:27808953-27808972 None:intergenic 35.0%
TAGATAGATATTTCGAGCAG+AGG + chr2.2:27808976-27808995 None:intergenic 35.0%
TCATGCTAAAAGAGAACAGA+AGG + chr2.2:27810572-27810591 None:intergenic 35.0%
TCCTTGTAACCAAAACCAAT+GGG + chr2.2:27809308-27809327 None:intergenic 35.0%
TCGAAATATCTATCTAGCAC+TGG - chr2.2:27808980-27808999 MS.gene043818:intron 35.0%
TCTTTCCACTTACATGAGAA+TGG - chr2.2:27809231-27809250 MS.gene043818:CDS 35.0%
TGAACTGGCATTATCATGAT+GGG - chr2.2:27809117-27809136 MS.gene043818:CDS 35.0%
TGATCTTGGAAAATTGATGG+AGG - chr2.2:27810249-27810268 MS.gene043818:intron 35.0%
TTCCTTGTAACCAAAACCAA+TGG + chr2.2:27809309-27809328 None:intergenic 35.0%
! ACATTGAGGATGTTTTGCAT+TGG - chr2.2:27809757-27809776 MS.gene043818:intron 35.0%
! CTTTATTTGTTCAGATCCGT+AGG - chr2.2:27810056-27810075 MS.gene043818:intron 35.0%
! TAGAGTGTTTGAGGTTTCAT+TGG - chr2.2:27809453-27809472 MS.gene043818:CDS 35.0%
! TGTTTCTATTTGGTCCTTGT+AGG - chr2.2:27810652-27810671 MS.gene043818:intron 35.0%
! TTTCATTGGCTGATCTTCAA+GGG - chr2.2:27809467-27809486 MS.gene043818:intron 35.0%
!! AATAATGTTGGTCTTGCTTG+TGG + chr2.2:27809077-27809096 None:intergenic 35.0%
!! ATCCAACACATGTTTTAGGT+AGG - chr2.2:27809921-27809940 MS.gene043818:intron 35.0%
!! TTTCTAAAGTATCGACTCCA+GGG - chr2.2:27809155-27809174 MS.gene043818:CDS 35.0%
!!! AAATCACTGGTTTTGAACCT+AGG - chr2.2:27809981-27810000 MS.gene043818:intron 35.0%
!!! AACCAAAGCCACTTTTTAGA+TGG + chr2.2:27810626-27810645 None:intergenic 35.0%
!!! GCTATTTAACCCATTGGTTT+TGG - chr2.2:27809296-27809315 MS.gene043818:intron 35.0%
!!! TTTTCTAAAGTATCGACTCC+AGG - chr2.2:27809154-27809173 MS.gene043818:CDS 35.0%
AAAGCTCCCAAGTTTGATCT+TGG - chr2.2:27810235-27810254 MS.gene043818:intron 40.0%
AACTGACAAACTGAGGTCAT+TGG - chr2.2:27809675-27809694 MS.gene043818:intron 40.0%
AAGAACAAGCGGATCTCTAA+GGG - chr2.2:27808155-27808174 MS.gene043818:CDS 40.0%
AATCTCCTTCCCAATCATCT+CGG + chr2.2:27810152-27810171 None:intergenic 40.0%
ACCATGCATTCAGGAAGATT+AGG - chr2.2:27809497-27809516 MS.gene043818:intron 40.0%
ACCTAATCTTCCTGAATGCA+TGG + chr2.2:27809501-27809520 None:intergenic 40.0%
AGAAGACAAAGCATGATCCT+AGG + chr2.2:27810001-27810020 None:intergenic 40.0%
AGCATCATCCTGTGATTTGA+AGG - chr2.2:27810105-27810124 MS.gene043818:intron 40.0%
AGCTATCTAAACAATCAGGG+AGG + chr2.2:27808514-27808533 None:intergenic 40.0%
ATCCATTTGCCAAGAAGGAT+TGG - chr2.2:27808298-27808317 MS.gene043818:intron 40.0%
ATTTGGTCCTTGTAGGTTCA+TGG - chr2.2:27810659-27810678 MS.gene043818:intron 40.0%
CAAGCTCCAACAATTTCAGT+AGG + chr2.2:27810749-27810768 None:intergenic 40.0%
CTGAACTGGCATTATCATGA+TGG - chr2.2:27809116-27809135 MS.gene043818:CDS 40.0%
CTGACTAGATTGTGCATAAC+AGG + chr2.2:27809818-27809837 None:intergenic 40.0%
GAATGACAGATTGCTTCAGA+TGG - chr2.2:27809421-27809440 MS.gene043818:CDS 40.0%
GACAGAGGAAAACAATACCT+TGG + chr2.2:27808397-27808416 None:intergenic 40.0%
GACCAATTCCTTCAAATCAC+AGG + chr2.2:27810116-27810135 None:intergenic 40.0%
GAGGAACCTACTGAAATTGT+TGG - chr2.2:27810740-27810759 MS.gene043818:CDS 40.0%
GATCAAACTTGGGAGCTTTA+AGG + chr2.2:27810234-27810253 None:intergenic 40.0%
GCTATCTAAACAATCAGGGA+GGG + chr2.2:27808513-27808532 None:intergenic 40.0%
GGTAGATCACATATCGCTAT+AGG + chr2.2:27808954-27808973 None:intergenic 40.0%
GTAATCTCCATGAACCTACA+AGG + chr2.2:27810669-27810688 None:intergenic 40.0%
GTCCTACCTAAAACATGTGT+TGG + chr2.2:27809926-27809945 None:intergenic 40.0%
TAAGAACAAGCGGATCTCTA+AGG - chr2.2:27808154-27808173 MS.gene043818:CDS 40.0%
TACCAATCCTTCTTGGCAAA+TGG + chr2.2:27808303-27808322 None:intergenic 40.0%
TCAAATCACAGGATGATGCT+TGG + chr2.2:27810105-27810124 None:intergenic 40.0%
TCATCTCGGGAATGAATTTG+CGG + chr2.2:27810138-27810157 None:intergenic 40.0%
TGAGAGCTGAAGATGTTCAA+GGG - chr2.2:27809521-27809540 MS.gene043818:intron 40.0%
TGATCTCAACCATCTTCCTA+CGG + chr2.2:27810075-27810094 None:intergenic 40.0%
TTCTTCACCTGGAAAACTGA+AGG + chr2.2:27808339-27808358 None:intergenic 40.0%
TTGAGAGCTGAAGATGTTCA+AGG - chr2.2:27809520-27809539 MS.gene043818:intron 40.0%
! ACTCAAGCATAGAGTGTTTG+AGG - chr2.2:27809444-27809463 MS.gene043818:CDS 40.0%
! AGTAGTCTTGACATCCACAT+GGG + chr2.2:27809728-27809747 None:intergenic 40.0%
! CGGTATCCAACACATGTTTT+AGG - chr2.2:27809917-27809936 MS.gene043818:intron 40.0%
! CTAGCTGCTATTTAACCCAT+TGG - chr2.2:27809290-27809309 MS.gene043818:intron 40.0%
! GAAACCGAATAGAGAGCTTT+TGG + chr2.2:27810433-27810452 None:intergenic 40.0%
! GTTTCATTGGCTGATCTTCA+AGG - chr2.2:27809466-27809485 MS.gene043818:intron 40.0%
! GTTTTCCAGGTGAAGAATGT+CGG - chr2.2:27808342-27808361 MS.gene043818:intron 40.0%
! TTCATTGGCTGATCTTCAAG+GGG - chr2.2:27809468-27809487 MS.gene043818:intron 40.0%
!! CAATGGGTTAAATAGCAGCT+AGG + chr2.2:27809292-27809311 None:intergenic 40.0%
!! TTCTAAAGTATCGACTCCAG+GGG - chr2.2:27809156-27809175 MS.gene043818:CDS 40.0%
!! TTGGTTGTTACTTTGGTCAC+TGG - chr2.2:27810353-27810372 MS.gene043818:intron 40.0%
!! TTGTCAAATAGCGGGTAAAG+TGG + chr2.2:27808841-27808860 None:intergenic 40.0%
!! TTTGCATTGGCTTTACCAAG+AGG - chr2.2:27809770-27809789 MS.gene043818:intron 40.0%
!!! ACCCATTGGTTTTGGTTACA+AGG - chr2.2:27809304-27809323 MS.gene043818:intron 40.0%
AACTCTTGTTGAAGCCCATG+TGG - chr2.2:27809711-27809730 MS.gene043818:intron 45.0%
ACATGTGTTGGATACCGTGT+TGG + chr2.2:27809914-27809933 None:intergenic 45.0%
AGAACAAGCGGATCTCTAAG+GGG - chr2.2:27808156-27808175 MS.gene043818:CDS 45.0%
ATCTCCTTCCCAATCATCTC+GGG + chr2.2:27810151-27810170 None:intergenic 45.0%
ATGTTGGCACAAAGGTAGAG+AGG - chr2.2:27810696-27810715 MS.gene043818:intron 45.0%
CAAGGGGTAAATACTAGACG+TGG + chr2.2:27810182-27810201 None:intergenic 45.0%
CGGATGAACACATTCTGCAA+GGG + chr2.2:27810199-27810218 None:intergenic 45.0%
CTTGTAGTGCAGAAAACAGC+CGG - chr2.2:27808780-27808799 MS.gene043818:CDS 45.0%
GATCCGCTTGTTCTTACCAA+CGG + chr2.2:27808150-27808169 None:intergenic 45.0%
GGATGAACACATTCTGCAAG+GGG + chr2.2:27810198-27810217 None:intergenic 45.0%
GGTTCATGGAGATTACTCTG+AGG - chr2.2:27810673-27810692 MS.gene043818:intron 45.0%
TCGGATGAACACATTCTGCA+AGG + chr2.2:27810200-27810219 None:intergenic 45.0%
TGCTGATCCATTTGCCAAGA+AGG - chr2.2:27808293-27808312 MS.gene043818:intron 45.0%
TGTTCAGATCCGTAGGAAGA+TGG - chr2.2:27810063-27810082 MS.gene043818:intron 45.0%
! ATCTCTAAGGGGAAGAAAGG+TGG - chr2.2:27808167-27808186 MS.gene043818:CDS 45.0%
! CAGTAGTCTTGACATCCACA+TGG + chr2.2:27809729-27809748 None:intergenic 45.0%
! GGAGATTACTCTGAGGATGT+TGG - chr2.2:27810680-27810699 MS.gene043818:intron 45.0%
! TAAGGCTCCTTCAGTTTTCC+AGG - chr2.2:27808329-27808348 MS.gene043818:intron 45.0%
!! ATATTATGAAAATAAATATT+GGG + chr2.2:27810317-27810336 None:intergenic 5.0%
!!! ATTTTTTTATTTTATTTACA+GGG - chr2.2:27809640-27809659 MS.gene043818:intron 5.0%
AGAGAGGCCTGCTGATGAAA+TGG - chr2.2:27810712-27810731 MS.gene043818:CDS 50.0%
CATAACAGGTCCTCTTCACC+TGG + chr2.2:27809804-27809823 None:intergenic 50.0%
CATTCCCGAGATGATTGGGA+AGG - chr2.2:27810144-27810163 MS.gene043818:intron 50.0%
GCCTGCTGATGAAATGGTGA+CGG - chr2.2:27810718-27810737 MS.gene043818:CDS 50.0%
TACCAAGAGGAGAGCTAACC+AGG - chr2.2:27809783-27809802 MS.gene043818:intron 50.0%
TCCGTCACCATTTCATCAGC+AGG + chr2.2:27810722-27810741 None:intergenic 50.0%
TCTTGCCGACATTCTTCACC+TGG + chr2.2:27808350-27808369 None:intergenic 50.0%
TGAGGTCATTGGTGCGTAAG+TGG - chr2.2:27809686-27809705 MS.gene043818:intron 50.0%
! CGGATCTCTAAGGGGAAGAA+AGG - chr2.2:27808164-27808183 MS.gene043818:CDS 50.0%
! CTCTGAGGATGTTGGCACAA+AGG - chr2.2:27810688-27810707 MS.gene043818:intron 50.0%
! TGGCCGTTGGTAAGAACAAG+CGG - chr2.2:27808144-27808163 MS.gene043818:CDS 50.0%
!! ATGTTGGTCTTGCTTGTGGC+AGG + chr2.2:27809073-27809092 None:intergenic 50.0%
!! TGCTGATGAAATGGTGACGG+AGG - chr2.2:27810721-27810740 MS.gene043818:CDS 50.0%
!! TGTTGGTCTTGCTTGTGGCA+GGG + chr2.2:27809072-27809091 None:intergenic 50.0%
ACCCTCGTGTCTCGTACTCA+GGG - chr2.2:27808369-27808388 MS.gene043818:intron 55.0%
ACCCTGAGTACGAGACACGA+GGG + chr2.2:27808373-27808392 None:intergenic 55.0%
CATGGGAATGTCATGTCCCC+TGG + chr2.2:27809175-27809194 None:intergenic 55.0%
GGAGAGCTAACCAGGTGAAG+AGG - chr2.2:27809791-27809810 MS.gene043818:intron 55.0%
GGGACGAAGACCATGCATTC+AGG - chr2.2:27809488-27809507 MS.gene043818:intron 55.0%
TACCCTGAGTACGAGACACG+AGG + chr2.2:27808374-27808393 None:intergenic 55.0%
! CACCTGGTTAGCTCTCCTCT+TGG + chr2.2:27809788-27809807 None:intergenic 55.0%
! GTCTCGTACTCAGGGTACCA+AGG - chr2.2:27808377-27808396 MS.gene043818:intron 55.0%
CTTGTGGCAGGGCAGAAGTG+GGG + chr2.2:27809061-27809080 None:intergenic 60.0%
GACCCTCGTGTCTCGTACTC+AGG - chr2.2:27808368-27808387 MS.gene043818:intron 60.0%
GCTTGTGGCAGGGCAGAAGT+GGG + chr2.2:27809062-27809081 None:intergenic 60.0%
TGCTTGTGGCAGGGCAGAAG+TGG + chr2.2:27809063-27809082 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 27808143 27810769 27808143 ID=MS.gene043818
chr2.2 mRNA 27808143 27810769 27808143 ID=MS.gene043818.t1;Parent=MS.gene043818
chr2.2 exon 27810711 27810769 27810711 ID=MS.gene043818.t1.exon1;Parent=MS.gene043818.t1
chr2.2 CDS 27810711 27810769 27810711 ID=cds.MS.gene043818.t1;Parent=MS.gene043818.t1
chr2.2 exon 27810514 27810619 27810514 ID=MS.gene043818.t1.exon2;Parent=MS.gene043818.t1
chr2.2 CDS 27810514 27810619 27810514 ID=cds.MS.gene043818.t1;Parent=MS.gene043818.t1
chr2.2 exon 27809345 27809482 27809345 ID=MS.gene043818.t1.exon3;Parent=MS.gene043818.t1
chr2.2 CDS 27809345 27809482 27809345 ID=cds.MS.gene043818.t1;Parent=MS.gene043818.t1
chr2.2 exon 27809066 27809251 27809066 ID=MS.gene043818.t1.exon4;Parent=MS.gene043818.t1
chr2.2 CDS 27809066 27809251 27809066 ID=cds.MS.gene043818.t1;Parent=MS.gene043818.t1
chr2.2 exon 27808642 27808842 27808642 ID=MS.gene043818.t1.exon5;Parent=MS.gene043818.t1
chr2.2 CDS 27808642 27808842 27808642 ID=cds.MS.gene043818.t1;Parent=MS.gene043818.t1
chr2.2 exon 27808143 27808238 27808143 ID=MS.gene043818.t1.exon6;Parent=MS.gene043818.t1
chr2.2 CDS 27808143 27808238 27808143 ID=cds.MS.gene043818.t1;Parent=MS.gene043818.t1
Gene Sequence

>MS.gene043818

ATGGCCGTTGGTAAGAACAAGCGGATCTCTAAGGGGAAGAAAGGTGGCAAGAAGAAAGCGTTAGTACCAACCCTTTTTCAAAATCCATTCAAATTTTCATTTTTTACAATTTTCCTTGCATTTTCTATAATGGTTTCTATGAACTTGCAGTGCTGATCCATTTGCCAAGAAGGATTGGTATGATATTAAGGCTCCTTCAGTTTTCCAGGTGAAGAATGTCGGCAAGACCCTCGTGTCTCGTACTCAGGGTACCAAGGTATTGTTTTCCTCTGTCATGTTTTTTGTAATTTGGTTTGATTTGTACATTGTTAGGTTATTGTACGAATTTATATGATCCTATCTTACTTTCATTGAATATCTATCTGGACCCTCCCTGATTGTTTAGATAGCTTAATTAAGAGCTTTTATATTTAAGTGTTGTTATATAAGTGTTTATGTATAAACTATTTTTATAACTAAAGATAGAATAAAGTCTAATTGTTTTCACATAAGCTAAAAGTTTTTCCTATAGGCTATTATGGAAATAAGCTGAAAAAACATCTCATGGATATGTCATAAGCTTTTTCGTTAAGCTCTTTCAAACCGTCACTTAGTGACATTATAGTGTTCGTGTTGTGTTTTTGTCAGTGTTCTCAATCTTGTAGTGCAGAAAACAGCCGGTTGTTAAAATTCTGCTACACAATAGTGCTATAGTGCCACTTTACCCGCTATTTGACAACATTTTTTTTGCTAAATAGTGTACCATAAAACAATTTTTATACTAAAAAGCATATCGTGGAACAATAGTGATTTGTATAAATTCAGCTACCCTATAGCGATATGTGATCTACCCTCTGCTCGAAATATCTATCTAGCACTGGGAGAGATGCGTCCACACTTATAAAACAACAATCATATCTTAAACTATCAGCTTGACCCCACTTCTGCCCTGCCACAAGCAAGACCAACATTATTTAAAAGACAAGAAAAAAGACTGAACTGGCATTATCATGATGGGCTCAAACTTAAATATTTTCTAAAGTATCGACTCCAGGGGACATGACATTCCCATGTCTTCATTTTTATTCTCGTGATTAGAGTGAGTGTCCTCTTTCCACTTACATGAGAATGGAAGTTTTAATGTTACATTGTAACATATATTCATTTCCTAGCTGCTATTTAACCCATTGGTTTTGGTTACAAGGAATAACAAATATGTCTTAGTCTTTCATGGGGTTGCTGAAATTTGATCAATTGGAGTTTTGCACGCTTAATGCTTATTTAGAAAATGGTTGTTTTGAATGACAGATTGCTTCAGATGGACTCAAGCATAGAGTGTTTGAGGTTTCATTGGCTGATCTTCAAGGGGACGAAGACCATGCATTCAGGAAGATTAGGTTGAGAGCTGAAGATGTTCAAGGGAAAAATCTTTTGACAAATTTCTGGGTATGATAAGCTGCCGAACCATCTATTTGTCATAATGTTGTTTGTTGGTATTTTTTTTTTAATTTGATTTTGATTTTTTTATTTTATTTACAGGGGATGAATTTCACAACTGACAAACTGAGGTCATTGGTGCGTAAGTGGCAAACTCTTGTTGAAGCCCATGTGGATGTCAAGACTACTGATAATTACACATTGAGGATGTTTTGCATTGGCTTTACCAAGAGGAGAGCTAACCAGGTGAAGAGGACCTGTTATGCACAATCTAGTCAGATTAGACAGGTAGATACATTGACGCTTTTCTTTTCAAATCATACTAGTTTTCTAGATTTATATATTAGTGTATCCAACACGGTATCCAACACATGTTTTAGGTAGGACCTTTCTTTTATGAAATGCTGTTATATATCAAGAAGAAATCACTGGTTTTGAACCTAGGATCATGCTTTGTCTTCTTCTAAGATATTTCTTATTAGTTACTAATGTTACACCTTTATTTGTTCAGATCCGTAGGAAGATGGTTGAGATCATGATCAACCAAGCATCATCCTGTGATTTGAAGGAATTGGTCCGCAAATTCATTCCCGAGATGATTGGGAAGGAGATTGAGAAAGCCACGTCTAGTATTTACCCCTTGCAGAATGTGTTCATCCGAAAAGTGAAGATCCTTAAAGCTCCCAAGTTTGATCTTGGAAAATTGATGGAGGTATTTTTTTGAAACAACTTTTTTATGTTTGTTCTCATTCACACCCAATATTTATTTTCATAATATCACCTGAATAAAAACTTTGGTTGTTACTTTGGTCACTGGTGTTATTTGCTTGACGAGATGAACTCTTTAATATAACCAATTACTTCAAACTTTCCAAAAGCTCTCTATTCGGTTTCTCCATAAACAACCTTCAGATTATTACAAGTTCAAAACTAAATACTTACTAATCCAATAAATGCTTGTGTGAAAACAAACTTAATCATGTTGCCTTTTAAAATTTTAATTTTATTTTCCTTCTGTTCTCTTTTAGCATGAGTATGCAGTTGTCACGTGTGCACACTTATAACCATCTAAAAAGTGGCTTTGGTTTGATTTTGTTTCTATTTGGTCCTTGTAGGTTCATGGAGATTACTCTGAGGATGTTGGCACAAAGGTAGAGAGGCCTGCTGATGAAATGGTGACGGAGGAACCTACTGAAATTGTTGGAGCTTGA
Protein sequence

>MS.gene043818.t1

MAVGKNKRISKGKKGGKKKAADPFAKKDWYDIKAPSVFQVKNVGKTLVSRTQGTKIASDGLKHRVFEVSLADLQGDEDHAFRKIRLRAEDVQGKNLLTNFWGMNFTTDKLRSLVRKWQTLVEAHVDVKTTDNYTLRMFCIGFTKRRANQVKRTCYAQSSQIRQIRRKMVEIMINQASSCDLKELVRKFIPEMIGKEIEKATSSIYPLQNVFIRKVKILKAPKFDLGKLMEVHGDYSEDVGTKVERPADEMVTEEPTEIVGA