AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028857

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028857.t1 MTR_2g090760 98.947 285 3 0 2 286 3 287 0.0 568
MS.gene028857.t1 MTR_4g078200 84.775 289 41 1 1 286 4 292 1.21e-179 496
MS.gene028857.t1 MTR_4g078200 89.565 230 24 0 57 286 2 231 8.19e-150 419
MS.gene028857.t1 MTR_3g008250 51.786 280 127 4 10 285 3 278 2.76e-92 275
MS.gene028857.t1 MTR_8g046300 53.755 253 114 2 25 274 20 272 3.18e-92 275
MS.gene028857.t1 MTR_5g093030 32.044 181 108 3 3 182 12 178 5.47e-26 101
MS.gene028857.t1 MTR_6g004320 35.075 134 49 1 95 228 1 96 2.98e-18 79.0
MS.gene028857.t1 MTR_5g093040 36.842 95 54 1 25 119 18 106 3.94e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028857.t1 AT1G03860 81.119 286 53 1 1 285 1 286 3.96e-166 462
MS.gene028857.t1 AT1G03860 81.119 286 53 1 1 285 1 286 3.96e-166 462
MS.gene028857.t1 AT4G28510 86.316 285 39 0 2 286 3 287 1.39e-165 461
MS.gene028857.t1 AT2G20530 81.469 286 52 1 1 286 1 285 1.64e-164 458
MS.gene028857.t1 AT2G20530 81.469 286 52 1 1 286 1 285 1.64e-164 458
MS.gene028857.t1 AT5G44140 76.259 278 65 1 1 277 1 278 4.92e-153 429
MS.gene028857.t1 AT1G03860 86.878 221 29 0 65 285 1 221 2.82e-133 376
MS.gene028857.t1 AT3G27280 51.838 272 126 2 15 285 12 279 6.42e-93 276
MS.gene028857.t1 AT3G27280 51.838 272 126 2 15 285 12 279 6.42e-93 276
MS.gene028857.t1 AT5G40770 53.257 261 121 1 15 274 12 272 2.95e-92 275
MS.gene028857.t1 AT5G14300 46.091 243 104 2 33 274 22 238 8.03e-72 221

Find 81 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACACTTTACAGGTGAAAAT+TGG 0.119569 2.3:+9546698 MS.gene028857:intron
TGCATCAACACTGGCGAAAT+TGG 0.141061 2.3:+9544742 MS.gene028857:CDS
CATTTGGTTGAGAGTACTTC+TGG 0.173279 2.3:+9545046 MS.gene028857:CDS
CTGGCGAAATTGGGAATTAT+TGG 0.260992 2.3:+9544752 MS.gene028857:CDS
TTGGGAATTATTGGTGGAAT+TGG 0.287106 2.3:+9544761 MS.gene028857:CDS
GCAAAAGATCACCTGCTTCC+AGG 0.365181 2.3:-9548039 None:intergenic
ATCACAAAATGTGTTCCTTC+AGG 0.369702 2.3:-9544977 None:intergenic
TTCTTGTGCAGCCACCTGCT+TGG 0.376184 2.3:-9547365 None:intergenic
CCCTCTCAGTCAGAATCTTC+CGG 0.382058 2.3:-9547261 None:intergenic
AAAGTTTCATGTATGATTGA+AGG 0.395524 2.3:-9546806 None:intergenic
TAAGTTGGCTGGCATTGTAC+TGG 0.400052 2.3:-9546844 None:intergenic
CAATGGCTTGTCCGATCAGT+TGG 0.421036 2.3:-9547928 None:intergenic
TGGAGAGAATTATAATGAAC+GGG 0.425726 2.3:+9546778 MS.gene028857:CDS
ACACTTTACAGGTGAAAATT+GGG 0.431100 2.3:+9546699 MS.gene028857:intron
GCATCAACACTGGCGAAATT+GGG 0.431369 2.3:+9544743 MS.gene028857:CDS
ATTTGGTTGAGAGTACTTCT+GGG 0.432938 2.3:+9545047 MS.gene028857:CDS
GCCAGCTACCTACAGTTTAT+CGG 0.442274 2.3:+9546750 MS.gene028857:CDS
TGTTCGTGCACGTCCTCATT+TGG 0.454482 2.3:+9545030 MS.gene028857:CDS
GTCCTTACTCGTCCTTTGCC+CGG 0.458860 2.3:+9546728 MS.gene028857:CDS
TTCCTCAGTGTGATGAAAGC+TGG 0.472587 2.3:-9547959 None:intergenic
TCAATTACTAGTCTGACCTT+TGG 0.487774 2.3:+9547316 MS.gene028857:CDS
TTCAGCAAACAAGGTTTACC+TGG 0.493035 2.3:+9548021 MS.gene028857:CDS
TGCTGCAATTGAAGCCAAGC+AGG 0.493081 2.3:+9547351 MS.gene028857:CDS
AACAGTCTTTACAATGTTGA+GGG 0.493383 2.3:+9544800 MS.gene028857:CDS
GAATCTTCAGCGGATCAACT+TGG 0.494004 2.3:+9548063 MS.gene028857:CDS
AAGTGCTGTAATTAGAGCAC+AGG 0.500292 2.3:+9547435 MS.gene028857:CDS
GGCCGGGCAAAGGACGAGTA+AGG 0.504744 2.3:-9546730 None:intergenic
GCGAAATTGGGAATTATTGG+TGG 0.507313 2.3:+9544755 MS.gene028857:CDS
ATCAAGAGCAATGTTGAAGT+TGG 0.509611 2.3:-9547287 None:intergenic
TGTGATGAAAGCTGGATTGT+TGG 0.511259 2.3:-9547951 None:intergenic
GTTGATGCAGCGCCACCACT+TGG 0.517803 2.3:-9544728 None:intergenic
CCTACAGTTTATCGGACCCT+TGG 0.517966 2.3:+9546758 MS.gene028857:CDS
AGGCTGTTAGTCGTGAAATC+CGG 0.519390 2.3:+9547242 MS.gene028857:intron
AATGGCTTGTCCGATCAGTT+GGG 0.519729 2.3:-9547927 None:intergenic
TAACAGTCTTTACAATGTTG+AGG 0.522899 2.3:+9544799 MS.gene028857:CDS
GTTCATTATAATTCTCTCCA+AGG 0.524825 2.3:-9546775 None:intergenic
ATCGTGTTTAACCGTCTCGT+TGG 0.527718 2.3:+9544836 MS.gene028857:CDS
GTTGTGTAGGTTTATCCTGA+AGG 0.531470 2.3:+9544962 MS.gene028857:intron
TATTGCAAATTCAGCAAACA+AGG 0.537639 2.3:+9548012 MS.gene028857:CDS
AACAAGGTTTACCTGGAAGC+AGG 0.540987 2.3:+9548028 MS.gene028857:CDS
CCTCCCGCTGTGTAATAAGT+TGG 0.543103 2.3:-9546859 None:intergenic
TCCGATAAACTGTAGGTAGC+TGG 0.544929 2.3:-9546751 None:intergenic
GTAGGTAGCTGGCCGGGCAA+AGG 0.546160 2.3:-9546740 None:intergenic
TTGTGATTCCGTGGTTTGAG+AGG 0.547203 2.3:+9544993 MS.gene028857:CDS
TTGGAGAGAATTATAATGAA+CGG 0.549347 2.3:+9546777 MS.gene028857:CDS
CCGCTGTGTAATAAGTTGGC+TGG 0.549719 2.3:-9546855 None:intergenic
ATCACCGGCCTCTCAAACCA+CGG 0.552231 2.3:-9545001 None:intergenic
AAGTTGGCTGGCATTGTACT+GGG 0.555741 2.3:-9546843 None:intergenic
TTCATTATAATTCTCTCCAA+GGG 0.556280 2.3:-9546774 None:intergenic
GAAGTACTCTCAACCAAATG+AGG 0.567361 2.3:-9545043 None:intergenic
TACTAGTCTGACCTTTGGCA+AGG 0.569845 2.3:+9547321 MS.gene028857:CDS
GATTCCGTGGTTTGAGAGGC+CGG 0.570682 2.3:+9544997 MS.gene028857:CDS
CAGCCAACTTATTACACAGC+GGG 0.571868 2.3:+9546856 MS.gene028857:CDS
GGCTGCACAAGAAGCTGAGA+GGG 0.572538 2.3:+9547375 MS.gene028857:CDS
TGGTGGCGCTGCATCAACAC+TGG 0.572841 2.3:+9544733 MS.gene028857:CDS
GCTAAAAGTGCCCAACTGAT+CGG 0.574265 2.3:+9547917 MS.gene028857:CDS
ATAAACTGTAGGTAGCTGGC+CGG 0.578623 2.3:-9546747 None:intergenic
TGAGAGGGCTAAATTTGTTG+TGG 0.593905 2.3:+9547390 MS.gene028857:CDS
TAAACTGTAGGTAGCTGGCC+GGG 0.598757 2.3:-9546746 None:intergenic
CCAAGGGTCCGATAAACTGT+AGG 0.602094 2.3:-9546758 None:intergenic
AACGCGAAAGTTCCAAGTGG+TGG 0.602185 2.3:+9544716 MS.gene028857:CDS
GAGGAAGATAGAAGCCGCGA+GGG 0.603991 2.3:+9547976 MS.gene028857:CDS
TGGGAGTCGTGATCTGCAGA+TGG 0.609717 2.3:+9545066 MS.gene028857:CDS
CCGGAAGATTCTGACTGAGA+GGG 0.615174 2.3:+9547261 MS.gene028857:CDS
TCCGGAAGATTCTGACTGAG+AGG 0.615920 2.3:+9547260 MS.gene028857:CDS
TGTCTTTGACACCAACGAGA+CGG 0.624230 2.3:-9544847 None:intergenic
TGCAATTGAAGCCAAGCAGG+TGG 0.632190 2.3:+9547354 MS.gene028857:CDS
GAAAGCTGGATTGTTGGCAA+TGG 0.633552 2.3:-9547945 None:intergenic
ACATGAAACTTTGAAAGCTG+TGG 0.633777 2.3:+9546817 MS.gene028857:CDS
TGGCTGCACAAGAAGCTGAG+AGG 0.639141 2.3:+9547374 MS.gene028857:CDS
GCACGAACATCATAGATCAC+CGG 0.645792 2.3:-9545016 None:intergenic
GACGGTTAAACACGATAGCA+CGG 0.646783 2.3:-9544829 None:intergenic
AGCAGTAAACTCCTTGCCAA+AGG 0.656274 2.3:-9547332 None:intergenic
AGTCTTTACAATGTTGAGGG+AGG 0.662722 2.3:+9544803 MS.gene028857:CDS
TCTCGTTGGTGTCAAAGACA+AGG 0.674892 2.3:+9544850 MS.gene028857:CDS
CCAACTTATTACACAGCGGG+AGG 0.682391 2.3:+9546859 MS.gene028857:CDS
TGAGGAAGATAGAAGCCGCG+AGG 0.696862 2.3:+9547975 MS.gene028857:CDS
AACATGTGCTATCTCCCTCG+CGG 0.711001 2.3:-9547990 None:intergenic
AAGAACGCGAAAGTTCCAAG+TGG 0.742151 2.3:+9544713 MS.gene028857:CDS
ATCCAGCTTTCATCACACTG+AGG 0.780168 2.3:+9547957 MS.gene028857:CDS
CCAGCCAACTTATTACACAG+CGG 0.790079 2.3:+9546855 MS.gene028857:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAACATTTCTAAATTTAA+AGG - chr2.3:9545815-9545834 None:intergenic 10.0%
!! TAAACATTTCTAAATTTAAA+GGG - chr2.3:9545814-9545833 None:intergenic 10.0%
!! GAATATGATATATCAAAAAT+GGG - chr2.3:9545925-9545944 None:intergenic 15.0%
!! GTTAAATGTTTCTTATAAAT+AGG - chr2.3:9546111-9546130 None:intergenic 15.0%
!! TGAATATGATATATCAAAAA+TGG - chr2.3:9545926-9545945 None:intergenic 15.0%
!! TTTGATATATCATATTCAAA+TGG + chr2.3:9545928-9545947 MS.gene028857:intron 15.0%
!!! AGTTTTAATGAATAATTCAT+AGG + chr2.3:9546560-9546579 MS.gene028857:intron 15.0%
!!! ATTATGTCTTCTAATATTTT+TGG + chr2.3:9546523-9546542 MS.gene028857:intron 15.0%
!!! TTTATTTTATCTTTAGCTAT+AGG - chr2.3:9545184-9545203 None:intergenic 15.0%
!! AAACATAATGAAACATATAG+AGG - chr2.3:9545585-9545604 None:intergenic 20.0%
!! AAATAATCTAACATAATGAC+AGG - chr2.3:9546970-9546989 None:intergenic 20.0%
!! ACATCCTACAATTTATTTAT+TGG + chr2.3:9547107-9547126 MS.gene028857:intron 20.0%
!! ATATATTTATAAAACTGGAG+AGG - chr2.3:9545768-9545787 None:intergenic 20.0%
!! ATGTTTCTTATAAATAGGAA+AGG - chr2.3:9546106-9546125 None:intergenic 20.0%
!! ATTCATGAAAATTATATCAC+TGG - chr2.3:9545610-9545629 None:intergenic 20.0%
!! ATTTACACATAATACTTTCA+AGG - chr2.3:9547044-9547063 None:intergenic 20.0%
!! CAAACCAATAAATAAATTGT+AGG - chr2.3:9547114-9547133 None:intergenic 20.0%
!! GAGAAAAATCTAAATACTAA+CGG - chr2.3:9546004-9546023 None:intergenic 20.0%
!! TTAATAGATCTATTCTTTGT+AGG + chr2.3:9547222-9547241 MS.gene028857:intron 20.0%
!! TTTGTGAATTGAAATGATAT+GGG + chr2.3:9544929-9544948 MS.gene028857:intron 20.0%
!!! AACTGTATTCAGATTTTAAA+AGG + chr2.3:9545645-9545664 MS.gene028857:intron 20.0%
!!! ATTTTCATGTAGAATACATT+CGG + chr2.3:9547527-9547546 MS.gene028857:intron 20.0%
!!! CGTTTTTACAAAGTTTATAT+TGG + chr2.3:9545720-9545739 MS.gene028857:intron 20.0%
!!! GTCAACTTTTTCTTATAAAT+AGG + chr2.3:9546210-9546229 MS.gene028857:intron 20.0%
!!! TGATTTGAAATAGTTCTTAA+GGG - chr2.3:9546292-9546311 None:intergenic 20.0%
!!! TTTTGTGAATTGAAATGATA+TGG + chr2.3:9544928-9544947 MS.gene028857:intron 20.0%
! AAAGTTTCATGTATGATTGA+AGG - chr2.3:9546809-9546828 None:intergenic 25.0%
! AATCTATAAACTTGAAGTAC+TGG - chr2.3:9546619-9546638 None:intergenic 25.0%
! CATTGCATAAATGATGTATT+TGG + chr2.3:9546141-9546160 MS.gene028857:intron 25.0%
! CTTATGTATAAGTTTGCATA+AGG + chr2.3:9545154-9545173 MS.gene028857:intron 25.0%
! TATCATATTCAAATGGTGTA+AGG + chr2.3:9545935-9545954 MS.gene028857:intron 25.0%
! TATTGATCAACATTGAATGT+TGG + chr2.3:9547830-9547849 MS.gene028857:intron 25.0%
! TCTAAATAAAACTGGATTTC+AGG - chr2.3:9545387-9545406 None:intergenic 25.0%
! TGAAAAAAGCTTATGCAAAA+TGG + chr2.3:9545273-9545292 MS.gene028857:intron 25.0%
! TTCATTATAATTCTCTCCAA+GGG - chr2.3:9546777-9546796 None:intergenic 25.0%
! TTCTTATAAATAGGAAAGGA+GGG - chr2.3:9546102-9546121 None:intergenic 25.0%
! TTGGAGAGAATTATAATGAA+CGG + chr2.3:9546777-9546796 MS.gene028857:CDS 25.0%
! TTTCATAAAACATATGCTAC+CGG - chr2.3:9547079-9547098 None:intergenic 25.0%
! TTTCTTAAACAATGACCTTA+GGG + chr2.3:9547736-9547755 MS.gene028857:intron 25.0%
! TTTCTTATAAATAGGAAAGG+AGG - chr2.3:9546103-9546122 None:intergenic 25.0%
!! ATACATAATGATTTTCAGAC+AGG - chr2.3:9545498-9545517 None:intergenic 25.0%
!! CTGATTTGAAATAGTTCTTA+AGG - chr2.3:9546293-9546312 None:intergenic 25.0%
!! CTTTTTCTTATAAATAGGAC+CGG + chr2.3:9546215-9546234 MS.gene028857:intron 25.0%
!! GATTTGAAATAGTTCTTAAG+GGG - chr2.3:9546291-9546310 None:intergenic 25.0%
!! TGGTTTGAATTCATTTTAAG+TGG + chr2.3:9547127-9547146 MS.gene028857:intron 25.0%
!! TTATTTTCATAAGTTCTCCA+AGG - chr2.3:9545251-9545270 None:intergenic 25.0%
!!! ATATTTTTGGATGTCAATGT+TGG + chr2.3:9546536-9546555 MS.gene028857:intron 25.0%
!!! GGTTTGAATTCATTTTAAGT+GGG + chr2.3:9547128-9547147 MS.gene028857:intron 25.0%
!!! TCTTTTGATTCCTTAAACAA+TGG - chr2.3:9547638-9547657 None:intergenic 25.0%
AAACAACAAAAACATGTACC+AGG - chr2.3:9547503-9547522 None:intergenic 30.0%
AACAGTCTTTACAATGTTGA+GGG + chr2.3:9544800-9544819 MS.gene028857:CDS 30.0%
AATAAGTTGTTCATCCAAAC+AGG - chr2.3:9545098-9545117 None:intergenic 30.0%
AATTGACTGAGATGTAAAAC+TGG - chr2.3:9547811-9547830 None:intergenic 30.0%
ACACTTTACAGGTGAAAATT+GGG + chr2.3:9546699-9546718 MS.gene028857:intron 30.0%
ACATTACTTTGTTAACTAGG+GGG - chr2.3:9545555-9545574 None:intergenic 30.0%
AGGACATTACTTTGTTAACT+AGG - chr2.3:9545558-9545577 None:intergenic 30.0%
AGTAACTGCATAAGAATTCA+CGG - chr2.3:9547471-9547490 None:intergenic 30.0%
AGTATGAATGATGCACATAA+CGG - chr2.3:9545525-9545544 None:intergenic 30.0%
ATAAGTTGTTCATCCAAACA+GGG - chr2.3:9545097-9545116 None:intergenic 30.0%
ATACCATCACAAAGAATAGA+GGG - chr2.3:9545474-9545493 None:intergenic 30.0%
ATGAAACATATAGAGGATTG+AGG - chr2.3:9545578-9545597 None:intergenic 30.0%
ATTCAGAGGCTAAACATAAA+AGG - chr2.3:9546434-9546453 None:intergenic 30.0%
ATTGGAGTTTACCTTTGTTA+AGG + chr2.3:9545738-9545757 MS.gene028857:intron 30.0%
CATGCAAGTCTAAATAAAAC+TGG - chr2.3:9545395-9545414 None:intergenic 30.0%
CATTGAGTAACTAATGTACT+TGG - chr2.3:9546180-9546199 None:intergenic 30.0%
CTTGATTTCGTTGAATTCAT+CGG + chr2.3:9544905-9544924 MS.gene028857:intron 30.0%
GACATTACTTTGTTAACTAG+GGG - chr2.3:9545556-9545575 None:intergenic 30.0%
GGACATTACTTTGTTAACTA+GGG - chr2.3:9545557-9545576 None:intergenic 30.0%
GTCATTGTTTAAGAAACTCA+AGG - chr2.3:9547732-9547751 None:intergenic 30.0%
GTTCATTATAATTCTCTCCA+AGG - chr2.3:9546778-9546797 None:intergenic 30.0%
GTTTCTTAAACAATGACCTT+AGG + chr2.3:9547735-9547754 MS.gene028857:intron 30.0%
TAACAGTCTTTACAATGTTG+AGG + chr2.3:9544799-9544818 MS.gene028857:CDS 30.0%
TATACCATCACAAAGAATAG+AGG - chr2.3:9545475-9545494 None:intergenic 30.0%
TATTGCAAATTCAGCAAACA+AGG + chr2.3:9548012-9548031 MS.gene028857:CDS 30.0%
TGGAGAGAATTATAATGAAC+GGG + chr2.3:9546778-9546797 MS.gene028857:CDS 30.0%
TGTAAATTCTTGTCATTACC+CGG + chr2.3:9547057-9547076 MS.gene028857:intron 30.0%
TTCAGAGGCTAAACATAAAA+GGG - chr2.3:9546433-9546452 None:intergenic 30.0%
TTCATAAAACATATGCTACC+GGG - chr2.3:9547078-9547097 None:intergenic 30.0%
TTGATGTGTAAAAAAGGTCA+GGG - chr2.3:9547888-9547907 None:intergenic 30.0%
! TCAACAATGATTTTGCAATC+AGG - chr2.3:9545854-9545873 None:intergenic 30.0%
!! AAACTTGAAGTACTGGAAAT+TGG - chr2.3:9546612-9546631 None:intergenic 30.0%
!! AAAGACAAGGTCATTTTCTA+GGG + chr2.3:9544863-9544882 MS.gene028857:intron 30.0%
!! ATTTTTCAAACCATGTTGCT+TGG + chr2.3:9546922-9546941 MS.gene028857:intron 30.0%
!! TTTTTCAAACCATGTTGCTT+GGG + chr2.3:9546923-9546942 MS.gene028857:intron 30.0%
!!! ACCTTTTTTACACATCAACA+GGG + chr2.3:9547890-9547909 MS.gene028857:intron 30.0%
!!! TTCCACATGAATTTGTTTTG+AGG + chr2.3:9545679-9545698 MS.gene028857:intron 30.0%
AAAGGGAAATTCAGACTACA+AGG - chr2.3:9546416-9546435 None:intergenic 35.0%
AACTCAAGGAAACTTTGTCT+AGG - chr2.3:9547718-9547737 None:intergenic 35.0%
ACATTTCAATCTTGTGATCC+TGG + chr2.3:9546035-9546054 MS.gene028857:intron 35.0%
ACCCTGTTGATGTGTAAAAA+AGG - chr2.3:9547894-9547913 None:intergenic 35.0%
AGGGAGTAGACAATAATCAT+CGG - chr2.3:9546083-9546102 None:intergenic 35.0%
ATCAAGAGCAATGTTGAAGT+TGG - chr2.3:9547290-9547309 None:intergenic 35.0%
ATCACAAAATGTGTTCCTTC+AGG - chr2.3:9544980-9544999 None:intergenic 35.0%
ATTCCTTAAACAATGGCCTA+AGG - chr2.3:9547631-9547650 None:intergenic 35.0%
CAACAATTAAATACTCCCTC+CGG - chr2.3:9546237-9546256 None:intergenic 35.0%
CACACTTTACAGGTGAAAAT+TGG + chr2.3:9546698-9546717 MS.gene028857:intron 35.0%
CATTACTTTGTTAACTAGGG+GGG - chr2.3:9545554-9545573 None:intergenic 35.0%
CTCCTCAAAACAAATTCATG+TGG - chr2.3:9545684-9545703 None:intergenic 35.0%
GTCTGTGTGGTGATTTAAAA+TGG + chr2.3:9546346-9546365 MS.gene028857:intron 35.0%
GTTATGTGCATCATTCATAC+TGG + chr2.3:9545524-9545543 MS.gene028857:intron 35.0%
GTTGATGTGTAAAAAAGGTC+AGG - chr2.3:9547889-9547908 None:intergenic 35.0%
TAGACTTGCATGTCATCATT+AGG + chr2.3:9545403-9545422 MS.gene028857:intron 35.0%
TATAGTTAAGTCCAAATGCC+AGG - chr2.3:9546056-9546075 None:intergenic 35.0%
TCATAGGTAGCAAAGATGTT+TGG + chr2.3:9546576-9546595 MS.gene028857:intron 35.0%
TGAGTATGATGCAAATTCAG+AGG - chr2.3:9546448-9546467 None:intergenic 35.0%
TGATGTGTAAAAAAGGTCAG+GGG - chr2.3:9547887-9547906 None:intergenic 35.0%
TTAAATCACCACACAGACAA+CGG - chr2.3:9546344-9546363 None:intergenic 35.0%
TTCCTTAAACAATGGCCTAA+GGG - chr2.3:9547630-9547649 None:intergenic 35.0%
TTCTTATAAATAGGACCGGA+GGG + chr2.3:9546219-9546238 MS.gene028857:intron 35.0%
TTGAGCTAATCTCTTGACAT+AGG - chr2.3:9545341-9545360 None:intergenic 35.0%
TTGGGAATTATTGGTGGAAT+TGG + chr2.3:9544761-9544780 MS.gene028857:CDS 35.0%
TTTCCCTCTATTCTTTGTGA+TGG + chr2.3:9545468-9545487 MS.gene028857:intron 35.0%
TTTCTTATAAATAGGACCGG+AGG + chr2.3:9546218-9546237 MS.gene028857:intron 35.0%
! ACATGAAACTTTGAAAGCTG+TGG + chr2.3:9546817-9546836 MS.gene028857:CDS 35.0%
! ATCTTTTGCTGAATCTTCAG+CGG + chr2.3:9548053-9548072 MS.gene028857:CDS 35.0%
! GAAGTATTTGAACAACTGCT+AGG + chr2.3:9545872-9545891 MS.gene028857:intron 35.0%
! TCAATTACTAGTCTGACCTT+TGG + chr2.3:9547316-9547335 MS.gene028857:CDS 35.0%
! TTTTGATGCCGTTAAACATC+TGG + chr2.3:9546383-9546402 MS.gene028857:intron 35.0%
!! AGATAAGACAGTGCTAGTTT+TGG - chr2.3:9547583-9547602 None:intergenic 35.0%
!! ATTTGGTTGAGAGTACTTCT+GGG + chr2.3:9545047-9545066 MS.gene028857:CDS 35.0%
!! CAAAGACAAGGTCATTTTCT+AGG + chr2.3:9544862-9544881 MS.gene028857:intron 35.0%
!!! AAGGTCATTTTCTAGGGTTT+AGG + chr2.3:9544869-9544888 MS.gene028857:intron 35.0%
!!! AGGTCATTTTCTAGGGTTTA+GGG + chr2.3:9544870-9544889 MS.gene028857:intron 35.0%
!!! ATAAGACAGTGCTAGTTTTG+GGG - chr2.3:9547581-9547600 None:intergenic 35.0%
!!! GACCTTTTTTACACATCAAC+AGG + chr2.3:9547889-9547908 MS.gene028857:intron 35.0%
!!! GATAAGACAGTGCTAGTTTT+GGG - chr2.3:9547582-9547601 None:intergenic 35.0%
AACTGGATTTCAGGCATGTT+TGG - chr2.3:9545378-9545397 None:intergenic 40.0%
AATCTTGTGATCCTGGCATT+TGG + chr2.3:9546042-9546061 MS.gene028857:intron 40.0%
ACCTGTAAAGTGTGTACATG+CGG - chr2.3:9546692-9546711 None:intergenic 40.0%
AGTCTTTACAATGTTGAGGG+AGG + chr2.3:9544803-9544822 MS.gene028857:CDS 40.0%
ATCAAACAACCCTGTGAAAG+TGG - chr2.3:9546498-9546517 None:intergenic 40.0%
ATGTGTAAAAAAGGTCAGGG+GGG - chr2.3:9547885-9547904 None:intergenic 40.0%
CTGGCGAAATTGGGAATTAT+TGG + chr2.3:9544752-9544771 MS.gene028857:CDS 40.0%
GATGTGTAAAAAAGGTCAGG+GGG - chr2.3:9547886-9547905 None:intergenic 40.0%
GATTTCAGGCATGTTTGGAT+TGG - chr2.3:9545373-9545392 None:intergenic 40.0%
GCCAATCACCAGATGTTTAA+CGG - chr2.3:9546394-9546413 None:intergenic 40.0%
GCGAAATTGGGAATTATTGG+TGG + chr2.3:9544755-9544774 MS.gene028857:CDS 40.0%
GTATTTGAACAACTGCTAGG+TGG + chr2.3:9545875-9545894 MS.gene028857:intron 40.0%
GTTGTGTAGGTTTATCCTGA+AGG + chr2.3:9544962-9544981 MS.gene028857:intron 40.0%
TCAAACAACCCTGTGAAAGT+GGG - chr2.3:9546497-9546516 None:intergenic 40.0%
TGTGATGAAAGCTGGATTGT+TGG - chr2.3:9547954-9547973 None:intergenic 40.0%
TTCAGCAAACAAGGTTTACC+TGG + chr2.3:9548021-9548040 MS.gene028857:CDS 40.0%
TTCTTATGCAGTTACTGCTG+TGG + chr2.3:9547475-9547494 MS.gene028857:intron 40.0%
! AAGTGCTGTAATTAGAGCAC+AGG + chr2.3:9547435-9547454 MS.gene028857:CDS 40.0%
! CTCTTGACATAGGTATTGTG+AGG - chr2.3:9545331-9545350 None:intergenic 40.0%
! GAACACATTTTGTGATTCCG+TGG + chr2.3:9544984-9545003 MS.gene028857:CDS 40.0%
! GAAGTACTCTCAACCAAATG+AGG - chr2.3:9545046-9545065 None:intergenic 40.0%
! TTTTACACATCAACAGGGTG+AGG + chr2.3:9547895-9547914 MS.gene028857:intron 40.0%
!! CATTTGGTTGAGAGTACTTC+TGG + chr2.3:9545046-9545065 MS.gene028857:CDS 40.0%
!! GCTGTTTTCATAAGCTACCT+TGG + chr2.3:9545231-9545250 MS.gene028857:intron 40.0%
!! TGAGAGGGCTAAATTTGTTG+TGG + chr2.3:9547390-9547409 MS.gene028857:CDS 40.0%
!!! GGTCATTTTCTAGGGTTTAG+GGG + chr2.3:9544871-9544890 MS.gene028857:intron 40.0%
AACAAGGTTTACCTGGAAGC+AGG + chr2.3:9548028-9548047 MS.gene028857:CDS 45.0%
AACACGTCATGCTAACCCTA+AGG - chr2.3:9547754-9547773 None:intergenic 45.0%
AAGAACGCGAAAGTTCCAAG+TGG + chr2.3:9544713-9544732 MS.gene028857:CDS 45.0%
AAGTTGGCTGGCATTGTACT+GGG - chr2.3:9546846-9546865 None:intergenic 45.0%
ACCGCATGTACACACTTTAC+AGG + chr2.3:9546688-9546707 MS.gene028857:intron 45.0%
AGCAGTAAACTCCTTGCCAA+AGG - chr2.3:9547335-9547354 None:intergenic 45.0%
AGGCTGTTAGTCGTGAAATC+CGG + chr2.3:9547242-9547261 MS.gene028857:intron 45.0%
ATAAACTGTAGGTAGCTGGC+CGG - chr2.3:9546750-9546769 None:intergenic 45.0%
ATCCAGCTTTCATCACACTG+AGG + chr2.3:9547957-9547976 MS.gene028857:CDS 45.0%
ATCGTGTTTAACCGTCTCGT+TGG + chr2.3:9544836-9544855 MS.gene028857:CDS 45.0%
CAGCCAACTTATTACACAGC+GGG + chr2.3:9546856-9546875 MS.gene028857:CDS 45.0%
CCAGCCAACTTATTACACAG+CGG + chr2.3:9546855-9546874 MS.gene028857:CDS 45.0%
GAAAGCTGGATTGTTGGCAA+TGG - chr2.3:9547948-9547967 None:intergenic 45.0%
GAATCTTCAGCGGATCAACT+TGG + chr2.3:9548063-9548082 MS.gene028857:CDS 45.0%
GACGGTTAAACACGATAGCA+CGG - chr2.3:9544832-9544851 None:intergenic 45.0%
GATCATTTCCGTTGTCTGTG+TGG + chr2.3:9546333-9546352 MS.gene028857:intron 45.0%
GCACGAACATCATAGATCAC+CGG - chr2.3:9545019-9545038 None:intergenic 45.0%
GCATCAACACTGGCGAAATT+GGG + chr2.3:9544743-9544762 MS.gene028857:CDS 45.0%
GCCAGCTACCTACAGTTTAT+CGG + chr2.3:9546750-9546769 MS.gene028857:CDS 45.0%
GCCGTTAAACATCTGGTGAT+TGG + chr2.3:9546390-9546409 MS.gene028857:intron 45.0%
GGAGAGGCAATCCTTAACAA+AGG - chr2.3:9545752-9545771 None:intergenic 45.0%
TACTTTGTTAACTAGGGGGG+TGG - chr2.3:9545551-9545570 None:intergenic 45.0%
TCCGATAAACTGTAGGTAGC+TGG - chr2.3:9546754-9546773 None:intergenic 45.0%
TCCTTTCTCCCACTTTCACA+GGG + chr2.3:9546486-9546505 MS.gene028857:intron 45.0%
TGCATCAACACTGGCGAAAT+TGG + chr2.3:9544742-9544761 MS.gene028857:CDS 45.0%
TGCCCTTAGGCCATTGTTTA+AGG + chr2.3:9547625-9547644 MS.gene028857:intron 45.0%
TGTCTTTGACACCAACGAGA+CGG - chr2.3:9544850-9544869 None:intergenic 45.0%
TTCCTCAGTGTGATGAAAGC+TGG - chr2.3:9547962-9547981 None:intergenic 45.0%
TTGTGATTCCGTGGTTTGAG+AGG + chr2.3:9544993-9545012 MS.gene028857:CDS 45.0%
! AATGGCTTGTCCGATCAGTT+GGG - chr2.3:9547930-9547949 None:intergenic 45.0%
! CTTGATCATGAAGTGCCCTT+AGG + chr2.3:9547612-9547631 MS.gene028857:intron 45.0%
! TAAGTTGGCTGGCATTGTAC+TGG - chr2.3:9546847-9546866 None:intergenic 45.0%
! TACTAGTCTGACCTTTGGCA+AGG + chr2.3:9547321-9547340 MS.gene028857:CDS 45.0%
!! GCTAAAAGTGCCCAACTGAT+CGG + chr2.3:9547917-9547936 MS.gene028857:CDS 45.0%
!! GGGTGTGTTGTTTGTTGTGT+AGG + chr2.3:9544949-9544968 MS.gene028857:intron 45.0%
!! TCTCGTTGGTGTCAAAGACA+AGG + chr2.3:9544850-9544869 MS.gene028857:CDS 45.0%
!! TTTATATATATTTATAAAAC+TGG - chr2.3:9545773-9545792 None:intergenic 5.0%
AACATGTGCTATCTCCCTCG+CGG - chr2.3:9547993-9548012 None:intergenic 50.0%
AACGCGAAAGTTCCAAGTGG+TGG + chr2.3:9544716-9544735 MS.gene028857:CDS 50.0%
ACCCTGTGAAAGTGGGAGAA+AGG - chr2.3:9546490-9546509 None:intergenic 50.0%
CCAACTTATTACACAGCGGG+AGG + chr2.3:9546859-9546878 MS.gene028857:CDS 50.0%
CCAAGGGTCCGATAAACTGT+AGG - chr2.3:9546761-9546780 None:intergenic 50.0%
CCCTCTCAGTCAGAATCTTC+CGG - chr2.3:9547264-9547283 None:intergenic 50.0%
CCGGAAGATTCTGACTGAGA+GGG + chr2.3:9547261-9547280 MS.gene028857:CDS 50.0%
CCTACAGTTTATCGGACCCT+TGG + chr2.3:9546758-9546777 MS.gene028857:CDS 50.0%
CCTCCCGCTGTGTAATAAGT+TGG - chr2.3:9546862-9546881 None:intergenic 50.0%
GATACCTCACCCAAGCAACA+TGG - chr2.3:9546935-9546954 None:intergenic 50.0%
GCAAAAGATCACCTGCTTCC+AGG - chr2.3:9548042-9548061 None:intergenic 50.0%
GCAGATGGTAAGTCCCTGTT+TGG + chr2.3:9545081-9545100 MS.gene028857:intron 50.0%
GTCCTTTCTCCCACTTTCAC+AGG + chr2.3:9546485-9546504 MS.gene028857:intron 50.0%
TAAACTGTAGGTAGCTGGCC+GGG - chr2.3:9546749-9546768 None:intergenic 50.0%
TCCGGAAGATTCTGACTGAG+AGG + chr2.3:9547260-9547279 MS.gene028857:CDS 50.0%
TGCAATTGAAGCCAAGCAGG+TGG + chr2.3:9547354-9547373 MS.gene028857:CDS 50.0%
TGCTGCAATTGAAGCCAAGC+AGG + chr2.3:9547351-9547370 MS.gene028857:CDS 50.0%
TGTTCGTGCACGTCCTCATT+TGG + chr2.3:9545030-9545049 MS.gene028857:CDS 50.0%
! CAAACCATGTTGCTTGGGTG+AGG + chr2.3:9546928-9546947 MS.gene028857:intron 50.0%
! CAATGGCTTGTCCGATCAGT+TGG - chr2.3:9547931-9547950 None:intergenic 50.0%
! CCGCTGTGTAATAAGTTGGC+TGG - chr2.3:9546858-9546877 None:intergenic 50.0%
ATCACCGGCCTCTCAAACCA+CGG - chr2.3:9545004-9545023 None:intergenic 55.0%
GAGGAAGATAGAAGCCGCGA+GGG + chr2.3:9547976-9547995 MS.gene028857:CDS 55.0%
GATTCCGTGGTTTGAGAGGC+CGG + chr2.3:9544997-9545016 MS.gene028857:CDS 55.0%
GGCTGCACAAGAAGCTGAGA+GGG + chr2.3:9547375-9547394 MS.gene028857:CDS 55.0%
GTCCTTACTCGTCCTTTGCC+CGG + chr2.3:9546728-9546747 MS.gene028857:CDS 55.0%
TGAGGAAGATAGAAGCCGCG+AGG + chr2.3:9547975-9547994 MS.gene028857:CDS 55.0%
TGGCTGCACAAGAAGCTGAG+AGG + chr2.3:9547374-9547393 MS.gene028857:CDS 55.0%
TGGGAGTCGTGATCTGCAGA+TGG + chr2.3:9545066-9545085 MS.gene028857:CDS 55.0%
TTCTTGTGCAGCCACCTGCT+TGG - chr2.3:9547368-9547387 None:intergenic 55.0%
GCAGTTACTGCTGTGGTGCC+TGG + chr2.3:9547482-9547501 MS.gene028857:intron 60.0%
GTTGATGCAGCGCCACCACT+TGG - chr2.3:9544731-9544750 None:intergenic 60.0%
! TGGTGGCGCTGCATCAACAC+TGG + chr2.3:9544733-9544752 MS.gene028857:CDS 60.0%
GGCCGGGCAAAGGACGAGTA+AGG - chr2.3:9546733-9546752 None:intergenic 65.0%
GTAGGTAGCTGGCCGGGCAA+AGG - chr2.3:9546743-9546762 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 9544704 9548102 9544704 ID=MS.gene028857
chr2.3 mRNA 9544704 9548102 9544704 ID=MS.gene028857.t1;Parent=MS.gene028857
chr2.3 exon 9544704 9544871 9544704 ID=MS.gene028857.t1.exon1;Parent=MS.gene028857.t1
chr2.3 CDS 9544704 9544871 9544704 ID=cds.MS.gene028857.t1;Parent=MS.gene028857.t1
chr2.3 exon 9544971 9545087 9544971 ID=MS.gene028857.t1.exon2;Parent=MS.gene028857.t1
chr2.3 CDS 9544971 9545087 9544971 ID=cds.MS.gene028857.t1;Parent=MS.gene028857.t1
chr2.3 exon 9546710 9546880 9546710 ID=MS.gene028857.t1.exon3;Parent=MS.gene028857.t1
chr2.3 CDS 9546710 9546880 9546710 ID=cds.MS.gene028857.t1;Parent=MS.gene028857.t1
chr2.3 exon 9547244 9547456 9547244 ID=MS.gene028857.t1.exon4;Parent=MS.gene028857.t1
chr2.3 CDS 9547244 9547456 9547244 ID=cds.MS.gene028857.t1;Parent=MS.gene028857.t1
chr2.3 exon 9547911 9548102 9547911 ID=MS.gene028857.t1.exon5;Parent=MS.gene028857.t1
chr2.3 CDS 9547911 9548102 9547911 ID=cds.MS.gene028857.t1;Parent=MS.gene028857.t1
Gene Sequence

>MS.gene028857

ATGAATTTTAAGAACGCGAAAGTTCCAAGTGGTGGCGCTGCATCAACACTGGCGAAATTGGGAATTATTGGTGGAATTGGTTTGTATGCTGCTGCTAACAGTCTTTACAATGTTGAGGGAGGTCACCGTGCTATCGTGTTTAACCGTCTCGTTGGTGTCAAAGACAAGGTCATTTTCTAGGGTTTAGGGGTTTGATTGTATCTTGATTTCGTTGAATTCATCGGTTTTGTGAATTGAAATGATATGGGTGTGTTGTTTGTTGTGTAGGTTTATCCTGAAGGAACACATTTTGTGATTCCGTGGTTTGAGAGGCCGGTGATCTATGATGTTCGTGCACGTCCTCATTTGGTTGAGAGTACTTCTGGGAGTCGTGATCTGCAGATGGTAAGTCCCTGTTTGGATGAACAACTTATTTGCAGCTTAAAGCATAAATACTTATCATAATAAACACTTATGTATAAGTTTGCATAAGGTTTTCCTATAGCTAAAGATAAAATAAAGTTAAATTGTTTTTGTATTTGTTTTAAGCTGTTTTCATAAGCTACCTTGGAGAACTTATGAAAATAAATTGAAAAAAGCTTATGCAAAATGGTGTAAGCTGTTATCTTAAGTTCTCTCAAGCAGCCTCACAATACCTATGTCAAGAGATTAGCTCAAATAAATAAGCCAATCCAAACATGCCTGAAATCCAGTTTTATTTAGACTTGCATGTCATCATTAGGATAAGTCTTTTCAACATAATAAATAACTTGCATATCTTCTTTTTTCCCTCTATTCTTTGTGATGGTATACCTGTCTGAAAATCATTATGTATTTTTCCGTTATGTGCATCATTCATACTGGACCACCCCCCTAGTTAACAAAGTAATGTCCTCAATCCTCTATATGTTTCATTATGTTTGACCAGTGATATAATTTTCATGAATGTGAAACAGATATAGAACTGTATTCAGATTTTAAAAGGTGCGTTCACAATTCCACATGAATTTGTTTTGAGGAGCTTATAATATAGACATCGTTTTTACAAAGTTTATATTGGAGTTTACCTTTGTTAAGGATTGCCTCTCCAGTTTTATAAATATATATAAACTCGTAAATGACAAGTCTCCCTTTAAATTTAGAAATGTTTATACATGTTTAGCTGATTCCTGATTGCAAAATCATTGTTGAAGTATTTGAACAACTGCTAGGTGGCTATTTTGTTATTTGCATAAAATGCCCATTTTTGATATATCATATTCAAATGGTGTAAGGCTTAAACATCAAAACATTTAGAAGTGTAATGATATAATTAAATCCGTTAGTATTTAGATTTTTCTCATTGATTGTAGACATTTCAATCTTGTGATCCTGGCATTTGGACTTAACTATACATGCCGATGATTATTGTCTACTCCCTCCTTTCCTATTTATAAGAAACATTTAACTTTTTTGATTCATTGCATAAATGATGTATTTGGTCTATATTCTATACCAAGTACATTAGTTACTCAATGAATCTCAAAAGTCAACTTTTTCTTATAAATAGGACCGGAGGGAGTATTTAATTGTTGTATCAGCTTAAACTTCAAAACATAACATGTGCCCCTTAAGAACTATTTCAAATCAGAAATGCTATAAAGATATCTTGATCATTTCCGTTGTCTGTGTGGTGATTTAAAATGGTTTCTTTTTGAACTTTTTGATGCCGTTAAACATCTGGTGATTGGCCTTGTAGTCTGAATTTCCCTTTTATGTTTAGCCTCTGAATTTGCATCATACTCACGTAATATCTGCAACATGTCCTTTCTCCCACTTTCACAGGGTTGTTTGATGACTTATTATGTCTTCTAATATTTTTGGATGTCAATGTTGGAAGTTTTAATGAATAATTCATAGGTAGCAAAGATGTTTGGTTTCATGAAACCAATTTCCAGTACTTCAAGTTTATAGATTATAGATGTTTTATAAATTTAAACATGTGCAATATTGATTTTTTCTTTAAACCGCATGTACACACTTTACAGGTGAAAATTGGGCTTCGAGTCCTTACTCGTCCTTTGCCCGGCCAGCTACCTACAGTTTATCGGACCCTTGGAGAGAATTATAATGAACGGGTTTTGCCTTCAATCATACATGAAACTTTGAAAGCTGTGGTTGCCCAGTACAATGCCAGCCAACTTATTACACAGCGGGAGGTAAGTAGAAAATCTAGTTGAGATGCATTAAAACTCAAGTTATTTTTCAAACCATGTTGCTTGGGTGAGGTATCTATTTTCTTTTGCCTGTCATTATGTTAGATTATTTTTGTTTCTATATTTGTCAGAAGCACGACGAATATACATACACATGATCTGTCCTTGAAAGTATTATGTGTAAATTCTTGTCATTACCCGGTAGCATATGTTTTATGAAATTAAAATCACATCCTACAATTTATTTATTGGTTTGAATTCATTTTAAGTGGGAAAGTATTATTGTTTTGTTTGTTTAATTGACATCACTGTTAATTTCAATTCTCAACAAAGAAGTATATGTTTTAATAGATCTATTCTTTGTAGGCTGTTAGTCGTGAAATCCGGAAGATTCTGACTGAGAGGGCAGCCAACTTCAACATTGCTCTTGATGATGTGTCAATTACTAGTCTGACCTTTGGCAAGGAGTTTACTGCTGCAATTGAAGCCAAGCAGGTGGCTGCACAAGAAGCTGAGAGGGCTAAATTTGTTGTGGAAAAAGCTGAGCAAGACAAGAGAAGTGCTGTAATTAGAGCACAGGTAAGAAGAAACCGTGAATTCTTATGCAGTTACTGCTGTGGTGCCTGGTACATGTTTTTGTTGTTTTTATATTTTCATGTAGAATACATTCGGAAAAAGTGTTACTTATTATTCTATCTAGCCCCAAAACTAGCACTGTCTTATCTATCTGTTCTCTTGATCATGAAGTGCCCTTAGGCCATTGTTTAAGGAATCAAAAGAAGTAGCTATTACATAGAAAATGATGTATTTATTACTTCAAGAGTTTGACTTGTATTTCCTAGACAAAGTTTCCTTGAGTTTCTTAAACAATGACCTTAGGGTTAGCATGACGTGTTAAAATTAAATATTAGTATGTAATATTTGCAATTGCCAGTTTTACATCTCAGTCAATTATTGATCAACATTGAATGTTGGAACATATTTTGCTTCTTAAATATTTAGTTCCCCCCTGACCTTTTTTACACATCAACAGGGTGAGGCTAAAAGTGCCCAACTGATCGGACAAGCCATTGCCAACAATCCAGCTTTCATCACACTGAGGAAGATAGAAGCCGCGAGGGAGATAGCACATGTTATTGCAAATTCAGCAAACAAGGTTTACCTGGAAGCAGGTGATCTTTTGCTGAATCTTCAGCGGATCAACTTGGATCCTAGCAAAAAATGA
Protein sequence

>MS.gene028857.t1

MNFKNAKVPSGGAASTLAKLGIIGGIGLYAAANSLYNVEGGHRAIVFNRLVGVKDKVYPEGTHFVIPWFERPVIYDVRARPHLVESTSGSRDLQMVKIGLRVLTRPLPGQLPTVYRTLGENYNERVLPSIIHETLKAVVAQYNASQLITQREAVSREIRKILTERAANFNIALDDVSITSLTFGKEFTAAIEAKQVAAQEAERAKFVVEKAEQDKRSAVIRAQGEAKSAQLIGQAIANNPAFITLRKIEAAREIAHVIANSANKVYLEAGDLLLNLQRINLDPSKK