AlfalfaGEDB | MS.gene86455

M. sativa cultivar XinJiangDaYe / MS.gene86455

KEGG: K02183
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF13499.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86455.t1	XP_003612498.1	86.5	200	27	0	1	200	1	200	3.50E-86	327.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86455.t1	Q93Z27	47.2	197	89	6	14	200	13	204	2.7e-34	146.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86455.t1	G7K424	86.5	200	27	0	1	200	1	200	2.6e-86	327.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04918	MS.gene86455	0.80672	7.17E-50	-1.69E-46
MS.gene04919	MS.gene86455	0.809303	2.02E-50	-1.69E-46
MS.gene04920	MS.gene86455	0.823823	1.13E-53	-1.69E-46
MS.gene049254	MS.gene86455	0.815078	1.11E-51	-1.69E-46
MS.gene049283	MS.gene86455	0.862926	3.59E-64	-1.69E-46
MS.gene049419	MS.gene86455	-0.826252	3.00E-54	-1.69E-46
MS.gene049452	MS.gene86455	0.807608	4.65E-50	-1.69E-46
MS.gene049499	MS.gene86455	0.81475	1.31E-51	-1.69E-46
MS.gene049983	MS.gene86455	0.865596	5.26E-65	-1.69E-46
MS.gene04998	MS.gene86455	0.838835	2.27E-57	-1.69E-46
MS.gene050271	MS.gene86455	0.828379	9.29E-55	-1.69E-46
MS.gene050414	MS.gene86455	0.809071	2.27E-50	-1.69E-46
MS.gene050646	MS.gene86455	0.810312	1.23E-50	-1.69E-46
MS.gene050654	MS.gene86455	0.838888	2.20E-57	-1.69E-46
MS.gene050759	MS.gene86455	0.816045	6.77E-52	-1.69E-46
MS.gene050941	MS.gene86455	0.806362	8.53E-50	-1.69E-46
MS.gene050962	MS.gene86455	0.801916	7.18E-49	-1.69E-46
MS.gene051122	MS.gene86455	0.806733	7.13E-50	-1.69E-46
MS.gene051275	MS.gene86455	0.830206	3.35E-55	-1.69E-46
MS.gene051343	MS.gene86455	0.815144	1.07E-51	-1.69E-46
MS.gene051597	MS.gene86455	0.803632	3.18E-49	-1.69E-46
MS.gene051676	MS.gene86455	0.807355	5.26E-50	-1.69E-46
MS.gene051831	MS.gene86455	0.801996	6.92E-49	-1.69E-46
MS.gene051975	MS.gene86455	0.806932	6.47E-50	-1.69E-46
MS.gene052118	MS.gene86455	0.806326	8.68E-50	-1.69E-46
MS.gene052533	MS.gene86455	0.803774	2.97E-49	-1.69E-46
MS.gene052685	MS.gene86455	0.802338	5.88E-49	-1.69E-46
MS.gene052826	MS.gene86455	0.800271	1.56E-48	-1.69E-46
MS.gene052954	MS.gene86455	0.821637	3.63E-53	-1.69E-46
MS.gene053006	MS.gene86455	0.83267	8.28E-56	-1.69E-46
MS.gene053094	MS.gene86455	0.802881	4.55E-49	-1.69E-46
MS.gene053295	MS.gene86455	0.800786	1.22E-48	-1.69E-46
MS.gene053298	MS.gene86455	0.857924	1.17E-62	-1.69E-46
MS.gene053564	MS.gene86455	0.812327	4.48E-51	-1.69E-46
MS.gene053575	MS.gene86455	0.816059	6.72E-52	-1.69E-46
MS.gene053726	MS.gene86455	0.810322	1.22E-50	-1.69E-46
MS.gene053879	MS.gene86455	0.805307	1.42E-49	-1.69E-46
MS.gene053931	MS.gene86455	0.819198	1.32E-52	-1.69E-46
MS.gene053963	MS.gene86455	0.861074	1.32E-63	-1.69E-46
MS.gene05410	MS.gene86455	0.805611	1.23E-49	-1.69E-46
MS.gene05415	MS.gene86455	0.804263	2.35E-49	-1.69E-46
MS.gene054222	MS.gene86455	0.818683	1.72E-52	-1.69E-46
MS.gene05439	MS.gene86455	0.826859	2.15E-54	-1.69E-46
MS.gene054479	MS.gene86455	0.824212	9.12E-54	-1.69E-46
MS.gene05469	MS.gene86455	0.800498	1.40E-48	-1.69E-46
MS.gene054892	MS.gene86455	0.877385	6.55E-69	-1.69E-46
MS.gene054893	MS.gene86455	0.883096	5.99E-71	-1.69E-46
MS.gene054957	MS.gene86455	0.811827	5.76E-51	-1.69E-46
MS.gene055150	MS.gene86455	0.813331	2.70E-51	-1.69E-46
MS.gene055222	MS.gene86455	0.846792	1.74E-59	-1.69E-46
MS.gene055226	MS.gene86455	0.829205	5.86E-55	-1.69E-46
MS.gene055841	MS.gene86455	0.830546	2.76E-55	-1.69E-46
MS.gene056699	MS.gene86455	0.808624	2.83E-50	-1.69E-46
MS.gene056776	MS.gene86455	0.813432	2.57E-51	-1.69E-46
MS.gene056779	MS.gene86455	0.809253	2.07E-50	-1.69E-46
MS.gene056834	MS.gene86455	0.800322	1.52E-48	-1.69E-46
MS.gene05686	MS.gene86455	0.802339	5.88E-49	-1.69E-46
MS.gene05690	MS.gene86455	0.80502	1.63E-49	-1.69E-46
MS.gene056973	MS.gene86455	0.814159	1.78E-51	-1.69E-46
MS.gene056986	MS.gene86455	0.803316	3.69E-49	-1.69E-46
MS.gene057102	MS.gene86455	0.802808	4.71E-49	-1.69E-46
MS.gene057155	MS.gene86455	0.849116	3.97E-60	-1.69E-46
MS.gene057208	MS.gene86455	0.858438	8.24E-63	-1.69E-46
MS.gene057622	MS.gene86455	0.850964	1.21E-60	-1.69E-46
MS.gene057890	MS.gene86455	-0.802665	5.04E-49	-1.69E-46
MS.gene057893	MS.gene86455	-0.801959	7.04E-49	-1.69E-46
MS.gene057999	MS.gene86455	0.82222	2.66E-53	-1.69E-46
MS.gene058008	MS.gene86455	0.805621	1.22E-49	-1.69E-46
MS.gene058159	MS.gene86455	0.817759	2.79E-52	-1.69E-46
MS.gene058223	MS.gene86455	-0.802172	6.37E-49	-1.69E-46
MS.gene058279	MS.gene86455	0.818831	1.60E-52	-1.69E-46
MS.gene058429	MS.gene86455	0.835551	1.57E-56	-1.69E-46
MS.gene058592	MS.gene86455	0.840582	7.97E-58	-1.69E-46
MS.gene058781	MS.gene86455	0.800402	1.47E-48	-1.69E-46
MS.gene05878	MS.gene86455	0.832377	9.79E-56	-1.69E-46
MS.gene059146	MS.gene86455	0.808312	3.29E-50	-1.69E-46
MS.gene059170	MS.gene86455	0.804512	2.09E-49	-1.69E-46
MS.gene05952	MS.gene86455	0.87142	6.93E-67	-1.69E-46
MS.gene05956	MS.gene86455	0.820671	6.06E-53	-1.69E-46
MS.gene05985	MS.gene86455	-0.829616	4.66E-55	-1.69E-46
MS.gene06023	MS.gene86455	0.804861	1.76E-49	-1.69E-46
MS.gene060259	MS.gene86455	0.878782	2.13E-69	-1.69E-46
MS.gene060365	MS.gene86455	0.818063	2.38E-52	-1.69E-46
MS.gene060412	MS.gene86455	0.844723	6.34E-59	-1.69E-46
MS.gene060413	MS.gene86455	0.802259	6.11E-49	-1.69E-46
MS.gene060520	MS.gene86455	0.808911	2.45E-50	-1.69E-46
MS.gene060840	MS.gene86455	0.821862	3.22E-53	-1.69E-46
MS.gene060850	MS.gene86455	0.806139	9.51E-50	-1.69E-46
MS.gene060851	MS.gene86455	0.817821	2.70E-52	-1.69E-46
MS.gene060852	MS.gene86455	0.81874	1.67E-52	-1.69E-46
MS.gene060932	MS.gene86455	0.827185	1.80E-54	-1.69E-46
MS.gene060933	MS.gene86455	0.80661	7.57E-50	-1.69E-46
MS.gene060935	MS.gene86455	0.822581	2.19E-53	-1.69E-46
MS.gene060937	MS.gene86455	0.830853	2.32E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86455.t1	MTR_5g025690	95.000	200	10	0	1	200	1	200	6.68e-134	373
MS.gene86455.t1	MTR_7g011010	78.641	206	34	2	1	200	1	202	4.17e-99	285
MS.gene86455.t1	MTR_4g127560	65.934	182	54	2	27	200	17	198	8.95e-72	216
MS.gene86455.t1	MTR_2g023310	86.275	51	7	0	83	133	47	97	2.72e-23	89.4
MS.gene86455.t1	MTR_4g082050	46.789	109	53	4	89	196	35	139	4.06e-20	82.8
MS.gene86455.t1	MTR_7g074240	53.846	65	30	0	131	195	106	170	4.29e-15	70.5
MS.gene86455.t1	MTR_4g115170	43.243	74	42	0	124	197	145	218	2.58e-12	63.9
MS.gene86455.t1	MTR_2g098890	45.902	61	33	0	136	196	143	203	1.96e-11	61.2
MS.gene86455.t1	MTR_7g089760	45.902	61	33	0	136	196	82	142	4.43e-11	58.9
MS.gene86455.t1	MTR_4g067270	41.791	67	39	0	131	197	77	143	4.82e-11	58.9
MS.gene86455.t1	MTR_3g109320	35.833	120	68	2	84	195	60	178	6.06e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86455.t1	AT5G39670	47.208	197	89	6	14	200	13	204	7.66e-46	150
MS.gene86455.t1	AT3G29000	60.185	108	41	1	89	196	85	190	9.64e-37	127
MS.gene86455.t1	AT3G47480	34.731	167	105	4	32	196	15	179	1.20e-15	72.0
MS.gene86455.t1	AT4G20780	54.930	71	29	1	125	195	116	183	3.05e-15	71.2
MS.gene86455.t1	AT5G44460	48.276	87	42	1	110	196	92	175	8.51e-15	69.7
MS.gene86455.t1	AT3G59440	26.904	197	101	5	29	197	7	188	2.67e-14	68.6
MS.gene86455.t1	AT2G43290	31.538	130	70	2	85	196	79	207	7.78e-14	67.8
MS.gene86455.t1	AT1G05990	46.154	65	35	0	133	197	80	144	1.19e-12	63.2
MS.gene86455.t1	AT3G07490	43.548	62	35	0	136	197	82	143	1.45e-11	60.5
MS.gene86455.t1	AT4G03290	45.312	64	35	0	133	196	82	145	1.93e-11	60.1

Find 23 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAAAGAGTTATGTGTATTT+TGG	0.141163	5.1:+16137377	MS.gene86455:CDS
TGAAACGGTGATGGCGAAAA+TGG	0.238809	5.1:+16137194	MS.gene86455:CDS
GAGAAAGTGGAGAGATTAAG+AGG	0.257266	5.1:+16137163	MS.gene86455:CDS
CTTCTCTTCCAACTTCGTTC+CGG	0.372796	5.1:+16137084	MS.gene86455:CDS
TGATGAGAATGAGCCAAGTT+TGG	0.401446	5.1:+16137287	MS.gene86455:CDS
ATCTTCACTTCCTCCAAACT+TGG	0.413419	5.1:-16137300	None:intergenic
GATGGAAGAATTGATTTCAC+TGG	0.420051	5.1:+16137465	MS.gene86455:CDS
CTAAGTCGCCGGAACGAAGT+TGG	0.452427	5.1:-16137092	None:intergenic
TTCCGGCGACTTAGAAGTAA+AGG	0.467315	5.1:+16137101	MS.gene86455:CDS
TGAAATCAATTCTTCCATCA+TGG	0.467568	5.1:-16137461	None:intergenic
TCCCTTTACTTCTAAGTCGC+CGG	0.486137	5.1:-16137103	None:intergenic
TCAAATGTTAGCAGAGAAAG+TGG	0.493623	5.1:+16137150	MS.gene86455:CDS
GAAACGGTGATGGCGAAAAT+GGG	0.493859	5.1:+16137195	MS.gene86455:CDS
AAGTTTGGAGGAAGTGAAGA+TGG	0.496634	5.1:+16137302	MS.gene86455:CDS
AGAAAGTGGAGAGATTAAGA+GGG	0.503717	5.1:+16137164	MS.gene86455:CDS
TGAGAATGAGCCAAGTTTGG+AGG	0.510803	5.1:+16137290	MS.gene86455:CDS
AGATGGATTTATTGATGCAA+AGG	0.523959	5.1:+16137350	MS.gene86455:CDS
TAAGAGGGATGAAGTTGAAA+CGG	0.526266	5.1:+16137179	MS.gene86455:CDS
ATTTGTTTGTCTTATTGAAG+AGG	0.533554	5.1:+16136933	MS.gene86455:CDS
CTTAGAAGTAAAGGGAGAGA+AGG	0.536217	5.1:+16137110	MS.gene86455:CDS
TCCGGCGACTTAGAAGTAAA+GGG	0.551355	5.1:+16137102	MS.gene86455:CDS
CGGAACGAAGTTGGAAGAGA+AGG	0.553899	5.1:-16137083	None:intergenic
GGATGAAGTTGAAACGGTGA+TGG	0.589256	5.1:+16137185	MS.gene86455:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAAAGTTTTTCTCAACTTTT+TGG	+	chr5.1:16137055-16137074	MS.gene86455:CDS	20.0%
!	CACTGGATTTGTAAAAATTA+TGG	+	chr5.1:16137482-16137501	MS.gene86455:CDS	25.0%
!	GATGAACTTGATGAAAAATA+TGG	+	chr5.1:16137240-16137259	MS.gene86455:CDS	25.0%
!	TGATGAAAAATATGGTTCAA+AGG	+	chr5.1:16137248-16137267	MS.gene86455:CDS	25.0%
!!	ATTTGTTTGTCTTATTGAAG+AGG	+	chr5.1:16136933-16136952	MS.gene86455:CDS	25.0%
!!	CAAAGAGTTATGTGTATTTT+GGG	+	chr5.1:16137378-16137397	MS.gene86455:CDS	25.0%
!!!	AATTTTGATGAAAACCATGA+TGG	+	chr5.1:16137447-16137466	MS.gene86455:CDS	25.0%
!!!	ACTTCATGTTCAACTTTTTT+TGG	+	chr5.1:16136988-16137007	MS.gene86455:CDS	25.0%
	TGAAATCAATTCTTCCATCA+TGG	-	chr5.1:16137464-16137483	None:intergenic	30.0%
!	AGATGGATTTATTGATGCAA+AGG	+	chr5.1:16137350-16137369	MS.gene86455:CDS	30.0%
!	GCAAAGAGTTATGTGTATTT+TGG	+	chr5.1:16137377-16137396	MS.gene86455:CDS	30.0%
!	GTTTTTGATGAGAACAAAGA+TGG	+	chr5.1:16137333-16137352	MS.gene86455:CDS	30.0%
!!!	TATGTGTATTTTGGGATTGA+AGG	+	chr5.1:16137386-16137405	MS.gene86455:CDS	30.0%
	AGAAAGTGGAGAGATTAAGA+GGG	+	chr5.1:16137164-16137183	MS.gene86455:CDS	35.0%
	TAAGAGGGATGAAGTTGAAA+CGG	+	chr5.1:16137179-16137198	MS.gene86455:CDS	35.0%
	TCAAATGTTAGCAGAGAAAG+TGG	+	chr5.1:16137150-16137169	MS.gene86455:CDS	35.0%
!	GATGGAAGAATTGATTTCAC+TGG	+	chr5.1:16137465-16137484	MS.gene86455:CDS	35.0%
!!!	TGTATTTTGGGATTGAAGGA+AGG	+	chr5.1:16137390-16137409	MS.gene86455:CDS	35.0%
	AAGTTTGGAGGAAGTGAAGA+TGG	+	chr5.1:16137302-16137321	MS.gene86455:CDS	40.0%
	ATCTTCACTTCCTCCAAACT+TGG	-	chr5.1:16137303-16137322	None:intergenic	40.0%
	CTTAGAAGTAAAGGGAGAGA+AGG	+	chr5.1:16137110-16137129	MS.gene86455:CDS	40.0%
	GAGAAAGTGGAGAGATTAAG+AGG	+	chr5.1:16137163-16137182	MS.gene86455:CDS	40.0%
	TGATGAGAATGAGCCAAGTT+TGG	+	chr5.1:16137287-16137306	MS.gene86455:CDS	40.0%
	CTTCTCTTCCAACTTCGTTC+CGG	+	chr5.1:16137084-16137103	MS.gene86455:CDS	45.0%
	GGATGAAGTTGAAACGGTGA+TGG	+	chr5.1:16137185-16137204	MS.gene86455:CDS	45.0%
	TCCCTTTACTTCTAAGTCGC+CGG	-	chr5.1:16137106-16137125	None:intergenic	45.0%
	TCCGGCGACTTAGAAGTAAA+GGG	+	chr5.1:16137102-16137121	MS.gene86455:CDS	45.0%
	TGAGAATGAGCCAAGTTTGG+AGG	+	chr5.1:16137290-16137309	MS.gene86455:CDS	45.0%
	TTCCGGCGACTTAGAAGTAA+AGG	+	chr5.1:16137101-16137120	MS.gene86455:CDS	45.0%
!	GAAACGGTGATGGCGAAAAT+GGG	+	chr5.1:16137195-16137214	MS.gene86455:CDS	45.0%
!	TGAAACGGTGATGGCGAAAA+TGG	+	chr5.1:16137194-16137213	MS.gene86455:CDS	45.0%
	CGGAACGAAGTTGGAAGAGA+AGG	-	chr5.1:16137086-16137105	None:intergenic	50.0%
	CTAAGTCGCCGGAACGAAGT+TGG	-	chr5.1:16137095-16137114	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	16136919	16137521	16136919	ID=MS.gene86455
chr5.1	mRNA	16136919	16137521	16136919	ID=MS.gene86455.t1;Parent=MS.gene86455
chr5.1	exon	16136919	16137521	16136919	ID=MS.gene86455.t1.exon1;Parent=MS.gene86455.t1
chr5.1	CDS	16136919	16137521	16136919	ID=cds.MS.gene86455.t1;Parent=MS.gene86455.t1

Gene Sequence

>MS.gene86455

ATGGTTTTTAGTCAATTTGTTTGTCTTATTGAAGAGGAGAAGACATCACAGAATGATGATAGCAACTCAACTTCATGTTCAACTTTTTTTGGTTTAATAGATTTTTTCATATATTCAACATTATTCATTAGAATACAAAAGTTTTTCTCAACTTTTTGGTTTTTCCTTCTCTTCCAACTTCGTTCCGGCGACTTAGAAGTAAAGGGAGAGAAGGATATATCAGAAACTGAATCAAATGTTAGCAGAGAAAGTGGAGAGATTAAGAGGGATGAAGTTGAAACGGTGATGGCGAAAATGGGAATTTTTTGCAGCTCAGAAAGTGATGAACTTGATGAAAAATATGGTTCAAAGGAACTTTCTGAATTGTTTGATGAGAATGAGCCAAGTTTGGAGGAAGTGAAGATGGCTTTTGATGTTTTTGATGAGAACAAAGATGGATTTATTGATGCAAAGGAATTGCAAAGAGTTATGTGTATTTTGGGATTGAAGGAAGGTTTAGAAGTTAAGAATTGTCAGAAGATGATCAAGAATTTTGATGAAAACCATGATGGAAGAATTGATTTCACTGGATTTGTAAAAATTATGGAAAACAGATTGTGTTGA

Protein sequence

>MS.gene86455.t1

MVFSQFVCLIEEEKTSQNDDSNSTSCSTFFGLIDFFIYSTLFIRIQKFFSTFWFFLLFQLRSGDLEVKGEKDISETESNVSRESGEIKRDEVETVMAKMGIFCSSESDELDEKYGSKELSELFDENEPSLEEVKMAFDVFDENKDGFIDAKELQRVMCILGLKEGLEVKNCQKMIKNFDENHDGRIDFTGFVKIMENRLC