AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63430


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63430.t1 MTR_3g067585 98.425 127 2 0 2 128 37 163 1.11e-92 265
MS.gene63430.t1 MTR_2g011590 82.677 127 22 0 2 128 36 162 1.58e-78 229
MS.gene63430.t1 MTR_8g107340 66.929 127 42 0 2 128 53 179 1.85e-63 191
MS.gene63430.t1 MTR_6g069470 63.780 127 46 0 2 128 49 175 5.74e-59 180
MS.gene63430.t1 MTR_1g031650 62.500 128 48 0 1 128 1 128 5.60e-58 176
MS.gene63430.t1 MTR_1g031650 62.205 127 48 0 2 128 39 165 1.57e-56 174
MS.gene63430.t1 MTR_4g084280 57.480 127 54 0 2 128 37 163 4.79e-50 157
MS.gene63430.t1 MTR_2g027100 40.496 121 70 1 2 120 37 157 3.42e-26 96.7
MS.gene63430.t1 MTR_8g038400 45.082 122 66 1 2 122 242 363 6.65e-26 100
MS.gene63430.t1 MTR_8g088320 39.516 124 74 1 2 124 197 320 8.88e-25 96.3
MS.gene63430.t1 MTR_2g030230 39.516 124 74 1 2 124 204 327 1.50e-24 95.9
MS.gene63430.t1 MTR_3g017500 42.857 126 63 2 2 122 245 366 1.43e-23 94.0
MS.gene63430.t1 MTR_3g017500 42.857 126 63 2 2 122 275 396 2.25e-23 93.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63430.t1 AT3G17980 65.354 127 44 0 2 128 49 175 4.52e-61 185
MS.gene63430.t1 AT1G48590 68.000 125 40 0 2 126 42 166 5.55e-61 185
MS.gene63430.t1 AT1G48590 68.000 125 40 0 2 126 42 166 5.55e-61 185
MS.gene63430.t1 AT1G48590 68.000 125 40 0 2 126 73 197 1.11e-60 185
MS.gene63430.t1 AT5G37740 63.846 130 44 1 2 128 37 166 2.63e-58 178
MS.gene63430.t1 AT5G37740 63.077 130 45 1 2 128 47 176 2.98e-57 176
MS.gene63430.t1 AT1G66360 60.938 128 48 1 2 127 37 164 3.19e-56 173
MS.gene63430.t1 AT1G73580 59.055 127 52 0 2 128 40 166 1.33e-55 171
MS.gene63430.t1 AT1G70810 62.992 127 47 0 2 128 37 163 2.24e-55 170
MS.gene63430.t1 AT1G70790 62.016 129 47 1 2 128 38 166 2.36e-52 163
MS.gene63430.t1 AT1G70790 62.016 129 47 1 2 128 38 166 2.36e-52 163
MS.gene63430.t1 AT1G70790 62.016 129 47 1 2 128 38 166 2.36e-52 163
MS.gene63430.t1 AT1G70790 61.719 128 47 1 3 128 2 129 6.83e-52 161
MS.gene63430.t1 AT2G01540 57.364 129 53 1 2 128 38 166 7.80e-50 157
MS.gene63430.t1 AT1G23140 55.906 127 56 0 2 128 37 163 6.45e-48 151
MS.gene63430.t1 AT1G70800 55.556 126 56 0 2 127 46 171 6.45e-48 152
MS.gene63430.t1 AT1G48590 61.682 107 38 1 23 126 59 165 1.76e-43 140
MS.gene63430.t1 AT3G07940 47.059 119 62 1 2 119 260 378 2.27e-27 104
MS.gene63430.t1 AT5G47710 37.500 128 78 1 2 127 37 164 1.08e-26 97.8
MS.gene63430.t1 AT5G47710 37.500 128 78 1 2 127 37 164 1.08e-26 97.8
MS.gene63430.t1 AT5G47710 37.500 128 78 1 2 127 37 164 1.08e-26 97.8
MS.gene63430.t1 AT5G47710 37.500 128 78 1 2 127 37 164 1.08e-26 97.8
MS.gene63430.t1 AT4G05330 40.164 122 72 1 2 122 176 297 2.99e-23 92.0
MS.gene63430.t1 AT4G05330 40.164 122 72 1 2 122 211 332 5.28e-23 92.0
MS.gene63430.t1 AT4G05330 40.164 122 72 1 2 122 211 332 5.28e-23 92.0
MS.gene63430.t1 AT4G21160 40.984 122 71 1 2 122 212 333 8.07e-23 91.3
MS.gene63430.t1 AT4G21160 40.984 122 71 1 2 122 212 333 8.07e-23 91.3
MS.gene63430.t1 AT4G21160 40.984 122 71 1 2 122 212 333 8.07e-23 91.3
MS.gene63430.t1 AT4G21160 40.984 122 71 1 2 122 212 333 8.07e-23 91.3

Find 37 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCAGCTTGGACGACAAAA+TGG 0.224120 3.4:-67989673 MS.gene63430:CDS
TTGCACTGGGTTGACATTCC+TGG 0.251961 3.4:-67989441 MS.gene63430:CDS
CAGAAGAGAGTCACATTGTT+TGG 0.286449 3.4:-67989536 MS.gene63430:CDS
TTAGTCTAAGAACCATGTTT+TGG 0.329440 3.4:+67989496 None:intergenic
CTTGTAACTATAGCCTCGTT+TGG 0.351980 3.4:+67989588 None:intergenic
GAATGTCAACCCAGTGCAAC+TGG 0.390071 3.4:+67989445 None:intergenic
AGTCACATTGTTTGGAAAGA+TGG 0.406065 3.4:-67989528 MS.gene63430:CDS
TTCAGCTTGGACGACAAAAT+GGG 0.451366 3.4:-67989672 MS.gene63430:CDS
TGGAGTATGAGGATCTGAAA+TGG 0.452578 3.4:+67990319 None:intergenic
AAGTGGAACTCCAGTTGCAC+TGG 0.453056 3.4:-67989455 MS.gene63430:CDS
TTGTAACTATAGCCTCGTTT+GGG 0.469930 3.4:+67989589 None:intergenic
GGTTGACATTCCTGGTTCAA+AGG 0.472769 3.4:-67989433 MS.gene63430:CDS
GCAGTAAAGATGCGGTTGGC+AGG 0.486595 3.4:-67989615 MS.gene63430:CDS
AAGTTACTTACTAGATGGAT+TGG 0.500412 3.4:+67990299 None:intergenic
GACTAAGAAATGTTGAATGT+GGG 0.501409 3.4:-67989479 MS.gene63430:CDS
GTTGACATTCCTGGTTCAAA+GGG 0.509321 3.4:-67989432 None:intergenic
CAAACGAGGCTATAGTTACA+AGG 0.509760 3.4:-67989587 MS.gene63430:CDS
ACTAGATGGATTGGAGTATG+AGG 0.510431 3.4:+67990308 None:intergenic
AAATGTTGAATGTGGGGAAG+TGG 0.522688 3.4:-67989472 MS.gene63430:CDS
AGACTAAGAAATGTTGAATG+TGG 0.533532 3.4:-67989480 MS.gene63430:CDS
AATGCAGTTTGTGTATGATA+AGG 0.538833 3.4:-67989700 MS.gene63430:intron
TGATGCAGAAGCTGAAGACC+AGG 0.545692 3.4:-67990391 MS.gene63430:CDS
GTCAAGTCCTCGTCCCACTC+AGG 0.564180 3.4:+67990347 None:intergenic
ACCTACATACCCTTTGAACC+AGG 0.570746 3.4:+67989423 None:intergenic
TGATAAGGACACATTCAGCT+TGG 0.578342 3.4:-67989685 MS.gene63430:CDS
TCTAAATCCTGAGTGGGACG+AGG 0.583435 3.4:-67990354 MS.gene63430:CDS
TGAAGCAGTAAAGATGCGGT+TGG 0.586646 3.4:-67989619 MS.gene63430:CDS
TCAGCTTGGACGACAAAATG+GGG 0.593533 3.4:-67989671 MS.gene63430:CDS
AAAGAATCTAAATCCTGAGT+GGG 0.594479 3.4:-67990360 MS.gene63430:CDS
AGTGGAACTCCAGTTGCACT+GGG 0.596489 3.4:-67989454 MS.gene63430:CDS
ACTAAGAAATGTTGAATGTG+GGG 0.600301 3.4:-67989478 MS.gene63430:CDS
AAAAGAATCTAAATCCTGAG+TGG 0.649840 3.4:-67990361 MS.gene63430:CDS
GTTGGCAGGTCTCCCAAACG+AGG 0.664369 3.4:-67989601 MS.gene63430:CDS
GGGGACGCTGAGTTTGACAT+CGG 0.670252 3.4:-67989651 MS.gene63430:CDS
AAACGAGGCTATAGTTACAA+GGG 0.687881 3.4:-67989586 MS.gene63430:CDS
GATGCAGAAGCTGAAGACCA+GGG 0.702840 3.4:-67990390 MS.gene63430:CDS
CAGCTTGGACGACAAAATGG+GGG 0.704105 3.4:-67989670 MS.gene63430:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGATAAATGAAAAATTT+AGG - chr3.4:67989586-67989605 MS.gene63430:CDS 10.0%
!! ACTATCTGAATATTAAAAAA+TGG - chr3.4:67990062-67990081 MS.gene63430:intron 15.0%
!!! CGAAAATAAGTTATATTTTA+GGG - chr3.4:67989785-67989804 MS.gene63430:intron 15.0%
!! ATCATGAACATTATCAAATA+TGG - chr3.4:67989810-67989829 MS.gene63430:intron 20.0%
!! TCATGAACATTATCAAATAT+GGG - chr3.4:67989811-67989830 MS.gene63430:intron 20.0%
!! TGCTTATTGTAATATGAATT+TGG - chr3.4:67989967-67989986 MS.gene63430:intron 20.0%
!! TTATTGTAATATGAATTTGG+TGG - chr3.4:67989970-67989989 MS.gene63430:intron 20.0%
!!! GCGAAAATAAGTTATATTTT+AGG - chr3.4:67989784-67989803 MS.gene63430:intron 20.0%
!!! TATAACTTATTTTCGCTATA+AGG + chr3.4:67989780-67989799 None:intergenic 20.0%
!!! TGTGTTTCAAATCAAAATTT+TGG - chr3.4:67989560-67989579 MS.gene63430:CDS 20.0%
! AAGAAAAGTTACTTACTAGA+TGG + chr3.4:67989531-67989550 None:intergenic 25.0%
! ATCTTTACTGCTTCAAAAAA+TGG + chr3.4:67990198-67990217 None:intergenic 25.0%
! CATGAACATTATCAAATATG+GGG - chr3.4:67989812-67989831 MS.gene63430:intron 25.0%
! TCTTTACTGCTTCAAAAAAT+GGG + chr3.4:67990197-67990216 None:intergenic 25.0%
!! AATTAGTTCAAGTTCTTACT+TGG - chr3.4:67989849-67989868 MS.gene63430:intron 25.0%
!! ACATGATAAGACTTTTCAAT+TGG - chr3.4:67989702-67989721 MS.gene63430:intron 25.0%
!! TGGTAAAATTTTCCAAAACA+TGG - chr3.4:67990314-67990333 MS.gene63430:CDS 25.0%
AAAAGAATCTAAATCCTGAG+TGG - chr3.4:67989461-67989480 MS.gene63430:CDS 30.0%
AAAGAATCTAAATCCTGAGT+GGG - chr3.4:67989462-67989481 MS.gene63430:CDS 30.0%
AAGTTACTTACTAGATGGAT+TGG + chr3.4:67989526-67989545 None:intergenic 30.0%
AATGCAGTTTGTGTATGATA+AGG - chr3.4:67990122-67990141 MS.gene63430:intron 30.0%
ACTAAGAAATGTTGAATGTG+GGG - chr3.4:67990344-67990363 MS.gene63430:CDS 30.0%
AGACTAAGAAATGTTGAATG+TGG - chr3.4:67990342-67990361 MS.gene63430:CDS 30.0%
GACTAAGAAATGTTGAATGT+GGG - chr3.4:67990343-67990362 MS.gene63430:CDS 30.0%
! GATATGAAATTTTCTCGCTT+TGG - chr3.4:67989618-67989637 MS.gene63430:CDS 30.0%
! TGTAATATGAATTTGGTGGA+AGG - chr3.4:67989974-67989993 MS.gene63430:intron 30.0%
! TTAGTCTAAGAACCATGTTT+TGG + chr3.4:67990329-67990348 None:intergenic 30.0%
!!! TTTTTGAAGCAGTAAAGATG+CGG - chr3.4:67990199-67990218 MS.gene63430:intron 30.0%
AAACGAGGCTATAGTTACAA+GGG - chr3.4:67990236-67990255 MS.gene63430:intron 35.0%
AGTCACATTGTTTGGAAAGA+TGG - chr3.4:67990294-67990313 MS.gene63430:intron 35.0%
TCTCATATTATCCGCTGTTA+AGG - chr3.4:67989876-67989895 MS.gene63430:intron 35.0%
TTGTAACTATAGCCTCGTTT+GGG + chr3.4:67990236-67990255 None:intergenic 35.0%
! CGTGTATTTTACCTTAACAG+CGG + chr3.4:67989890-67989909 None:intergenic 35.0%
! GAAATTTTCTCGCTTTGGTT+TGG - chr3.4:67989623-67989642 MS.gene63430:CDS 35.0%
!! TAGATTCTTTTTCACTACCC+TGG + chr3.4:67989452-67989471 None:intergenic 35.0%
AAATGTTGAATGTGGGGAAG+TGG - chr3.4:67990350-67990369 MS.gene63430:CDS 40.0%
ATTCAGCTTGGACGACAAAA+TGG - chr3.4:67990149-67990168 MS.gene63430:intron 40.0%
CAAACGAGGCTATAGTTACA+AGG - chr3.4:67990235-67990254 MS.gene63430:intron 40.0%
CAGAAGAGAGTCACATTGTT+TGG - chr3.4:67990286-67990305 MS.gene63430:intron 40.0%
CATACACAAACTGCATTGCA+AGG + chr3.4:67990119-67990138 None:intergenic 40.0%
CTTGTAACTATAGCCTCGTT+TGG + chr3.4:67990237-67990256 None:intergenic 40.0%
TGATAAGGACACATTCAGCT+TGG - chr3.4:67990137-67990156 MS.gene63430:intron 40.0%
TGGAGTATGAGGATCTGAAA+TGG + chr3.4:67989506-67989525 None:intergenic 40.0%
TTCAGCTTGGACGACAAAAT+GGG - chr3.4:67990150-67990169 MS.gene63430:intron 40.0%
! GCTAAGCAATTTTGTCTGCT+AGG + chr3.4:67990267-67990286 None:intergenic 40.0%
!! ACTAGATGGATTGGAGTATG+AGG + chr3.4:67989517-67989536 None:intergenic 40.0%
GGTTGACATTCCTGGTTCAA+AGG - chr3.4:67990389-67990408 MS.gene63430:CDS 45.0%
TCAGCTTGGACGACAAAATG+GGG - chr3.4:67990151-67990170 MS.gene63430:intron 45.0%
TGAAGCAGTAAAGATGCGGT+TGG - chr3.4:67990203-67990222 MS.gene63430:intron 45.0%
AAGTGGAACTCCAGTTGCAC+TGG - chr3.4:67990367-67990386 MS.gene63430:CDS 50.0%
AGTGGAACTCCAGTTGCACT+GGG - chr3.4:67990368-67990387 MS.gene63430:CDS 50.0%
CAGCTTGGACGACAAAATGG+GGG - chr3.4:67990152-67990171 MS.gene63430:intron 50.0%
GAATGTCAACCCAGTGCAAC+TGG + chr3.4:67990380-67990399 None:intergenic 50.0%
TCTAAATCCTGAGTGGGACG+AGG - chr3.4:67989468-67989487 MS.gene63430:CDS 50.0%
! TTGCACTGGGTTGACATTCC+TGG - chr3.4:67990381-67990400 MS.gene63430:CDS 50.0%
GCAGTAAAGATGCGGTTGGC+AGG - chr3.4:67990207-67990226 MS.gene63430:intron 55.0%
GGGGACGCTGAGTTTGACAT+CGG - chr3.4:67990171-67990190 MS.gene63430:intron 55.0%
GTCAAGTCCTCGTCCCACTC+AGG + chr3.4:67989478-67989497 None:intergenic 60.0%
GTTGGCAGGTCTCCCAAACG+AGG - chr3.4:67990221-67990240 MS.gene63430:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 67989433 67990411 67989433 ID=MS.gene63430
chr3.4 mRNA 67989433 67990411 67989433 ID=MS.gene63430.t1;Parent=MS.gene63430
chr3.4 exon 67990310 67990411 67990310 ID=MS.gene63430.t1.exon1;Parent=MS.gene63430.t1
chr3.4 CDS 67990310 67990411 67990310 ID=cds.MS.gene63430.t1;Parent=MS.gene63430.t1
chr3.4 exon 67989433 67989715 67989433 ID=MS.gene63430.t1.exon2;Parent=MS.gene63430.t1
chr3.4 CDS 67989433 67989715 67989433 ID=cds.MS.gene63430.t1;Parent=MS.gene63430.t1
Gene Sequence

>MS.gene63430

ATGCAGAAGCTGAAGACCAGGGTAGTGAAAAAGAATCTAAATCCTGAGTGGGACGAGGACTTGACTTTATCCATTTCAGATCCTCATACTCCAATCCATCTAGTAAGTAACTTTTCTTTAAACGTTGTGTGTTTCAAATCAAAATTTTGGTTAAAAAGATAAATGAAAAATTTAGGTTAGTTAAAGATATGAAATTTTCTCGCTTTGGTTTGGCTATCTGTATTATCATGTTGTTATTGATGCATATCACTATTATCATATGACAGAAAACATGATAAGACTTTTCAATTGGTAGAAATTTGATCTGTATATGACAGAACACATCTTAAATCATACAAACACTACCTTATAGCGAAAATAAGTTATATTTTAGGGAGATCATGAACATTATCAAATATGGGGTTTGTTGAATTTAGAATTAGTTCAAGTTCTTACTTGGTCTGTCTCATATTATCCGCTGTTAAGGTAAAATACACGTCTTCAGAAAATAATTTACTCTATTTTTTTATGTAAATCAATTTTTTGACTATATTGTGCTTATTGTAATATGAATTTGGTGGAAGGACTAATTAGTGTATGATTGAGAAGATATAAAAAAATAAGAATATTGATGAAAAATTGTAATTAGTACTATCTGAATATTAAAAAATGGAAAATAATTTGAGACAATATTCTAAACATGACCTTGCAATGCAGTTTGTGTATGATAAGGACACATTCAGCTTGGACGACAAAATGGGGGACGCTGAGTTTGACATCGGCCCATTTTTTGAAGCAGTAAAGATGCGGTTGGCAGGTCTCCCAAACGAGGCTATAGTTACAAGGGTGCAACCTAGCAGACAAAATTGCTTAGCAGAAGAGAGTCACATTGTTTGGAAAGATGGTAAAATTTTCCAAAACATGGTTCTTAGACTAAGAAATGTTGAATGTGGGGAAGTGGAACTCCAGTTGCACTGGGTTGACATTCCTGGTTCAAAGG

Protein sequence

>MS.gene63430.t1

MQKLKTRVVKKNLNPEWDEDLTLSISDPHTPIHLFVYDKDTFSLDDKMGDAEFDIGPFFEAVKMRLAGLPNEAIVTRVQPSRQNCLAEESHIVWKDGKIFQNMVLRLRNVECGEVELQLHWVDIPGSK