Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47311.t1 | XP_013451547.1 | 95.2 | 63 | 3 | 0 | 1 | 63 | 1 | 63 | 3.50E-10 | 74.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47311.t1 | I3SGW9 | 95.2 | 63 | 3 | 0 | 1 | 63 | 1 | 63 | 2.5e-10 | 74.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene47311 | 0.835684 | 1.45E-56 | -1.69E-46 |
| MS.gene04918 | MS.gene47311 | 0.801018 | 1.10E-48 | -1.69E-46 |
| MS.gene049368 | MS.gene47311 | 0.839714 | 1.34E-57 | -1.69E-46 |
| MS.gene049499 | MS.gene47311 | 0.811195 | 7.90E-51 | -1.69E-46 |
| MS.gene049786 | MS.gene47311 | 0.803801 | 2.93E-49 | -1.69E-46 |
| MS.gene049983 | MS.gene47311 | 0.802357 | 5.83E-49 | -1.69E-46 |
| MS.gene050084 | MS.gene47311 | 0.81281 | 3.51E-51 | -1.69E-46 |
| MS.gene050085 | MS.gene47311 | 0.838048 | 3.62E-57 | -1.69E-46 |
| MS.gene050384 | MS.gene47311 | 0.820395 | 7.02E-53 | -1.69E-46 |
| MS.gene050386 | MS.gene47311 | 0.824817 | 6.57E-54 | -1.69E-46 |
| MS.gene050654 | MS.gene47311 | 0.806857 | 6.71E-50 | -1.69E-46 |
| MS.gene050689 | MS.gene47311 | 0.809979 | 1.45E-50 | -1.69E-46 |
| MS.gene050752 | MS.gene47311 | 0.844212 | 8.69E-59 | -1.69E-46 |
| MS.gene050838 | MS.gene47311 | 0.810141 | 1.34E-50 | -1.69E-46 |
| MS.gene050941 | MS.gene47311 | 0.821902 | 3.15E-53 | -1.69E-46 |
| MS.gene050955 | MS.gene47311 | 0.809275 | 2.05E-50 | -1.69E-46 |
| MS.gene051003 | MS.gene47311 | 0.867902 | 9.71E-66 | -1.69E-46 |
| MS.gene051184 | MS.gene47311 | 0.803569 | 3.27E-49 | -1.69E-46 |
| MS.gene051234 | MS.gene47311 | 0.803216 | 3.87E-49 | -1.69E-46 |
| MS.gene051343 | MS.gene47311 | 0.847853 | 8.89E-60 | -1.69E-46 |
| MS.gene05134 | MS.gene47311 | 0.80282 | 4.68E-49 | -1.69E-46 |
| MS.gene051676 | MS.gene47311 | 0.822417 | 2.40E-53 | -1.69E-46 |
| MS.gene051856 | MS.gene47311 | 0.850242 | 1.93E-60 | -1.69E-46 |
| MS.gene051975 | MS.gene47311 | 0.83869 | 2.48E-57 | -1.69E-46 |
| MS.gene052072 | MS.gene47311 | 0.835391 | 1.72E-56 | -1.69E-46 |
| MS.gene052296 | MS.gene47311 | 0.821513 | 3.88E-53 | -1.69E-46 |
| MS.gene052342 | MS.gene47311 | 0.800724 | 1.26E-48 | -1.69E-46 |
| MS.gene052434 | MS.gene47311 | 0.815055 | 1.12E-51 | -1.69E-46 |
| MS.gene053058 | MS.gene47311 | 0.807303 | 5.40E-50 | -1.69E-46 |
| MS.gene053295 | MS.gene47311 | 0.817371 | 3.41E-52 | -1.69E-46 |
| MS.gene053564 | MS.gene47311 | 0.80984 | 1.55E-50 | -1.69E-46 |
| MS.gene053590 | MS.gene47311 | 0.82146 | 3.99E-53 | -1.69E-46 |
| MS.gene053931 | MS.gene47311 | 0.821689 | 3.53E-53 | -1.69E-46 |
| MS.gene054403 | MS.gene47311 | 0.841449 | 4.72E-58 | -1.69E-46 |
| MS.gene05469 | MS.gene47311 | 0.833595 | 4.87E-56 | -1.69E-46 |
| MS.gene05517 | MS.gene47311 | 0.829412 | 5.22E-55 | -1.69E-46 |
| MS.gene055311 | MS.gene47311 | 0.813078 | 3.07E-51 | -1.69E-46 |
| MS.gene055313 | MS.gene47311 | 0.806783 | 6.96E-50 | -1.69E-46 |
| MS.gene055513 | MS.gene47311 | 0.8096 | 1.75E-50 | -1.69E-46 |
| MS.gene056181 | MS.gene47311 | 0.852493 | 4.45E-61 | -1.69E-46 |
| MS.gene056699 | MS.gene47311 | 0.820664 | 6.09E-53 | -1.69E-46 |
| MS.gene056787 | MS.gene47311 | 0.842297 | 2.82E-58 | -1.69E-46 |
| MS.gene056986 | MS.gene47311 | 0.836298 | 1.02E-56 | -1.69E-46 |
| MS.gene057102 | MS.gene47311 | 0.810303 | 1.23E-50 | -1.69E-46 |
| MS.gene057140 | MS.gene47311 | 0.800986 | 1.11E-48 | -1.69E-46 |
| MS.gene057208 | MS.gene47311 | 0.808028 | 3.79E-50 | -1.69E-46 |
| MS.gene057234 | MS.gene47311 | 0.829101 | 6.21E-55 | -1.69E-46 |
| MS.gene057392 | MS.gene47311 | 0.809027 | 2.32E-50 | -1.69E-46 |
| MS.gene057694 | MS.gene47311 | 0.80061 | 1.33E-48 | -1.69E-46 |
| MS.gene057890 | MS.gene47311 | -0.807922 | 3.99E-50 | -1.69E-46 |
| MS.gene057999 | MS.gene47311 | 0.81456 | 1.45E-51 | -1.69E-46 |
| MS.gene058008 | MS.gene47311 | 0.817282 | 3.57E-52 | -1.69E-46 |
| MS.gene058009 | MS.gene47311 | 0.80471 | 1.90E-49 | -1.69E-46 |
| MS.gene058054 | MS.gene47311 | 0.828182 | 1.04E-54 | -1.69E-46 |
| MS.gene058055 | MS.gene47311 | 0.816748 | 4.71E-52 | -1.69E-46 |
| MS.gene058159 | MS.gene47311 | 0.800823 | 1.20E-48 | -1.69E-46 |
| MS.gene058292 | MS.gene47311 | 0.831045 | 2.08E-55 | -1.69E-46 |
| MS.gene058852 | MS.gene47311 | 0.818016 | 2.44E-52 | -1.69E-46 |
| MS.gene059146 | MS.gene47311 | 0.848604 | 5.51E-60 | -1.69E-46 |
| MS.gene059227 | MS.gene47311 | 0.810389 | 1.18E-50 | -1.69E-46 |
| MS.gene059360 | MS.gene47311 | 0.800586 | 1.34E-48 | -1.69E-46 |
| MS.gene059765 | MS.gene47311 | 0.809506 | 1.83E-50 | -1.69E-46 |
| MS.gene06010 | MS.gene47311 | 0.818919 | 1.52E-52 | -1.69E-46 |
| MS.gene06011 | MS.gene47311 | 0.811482 | 6.85E-51 | -1.69E-46 |
| MS.gene060244 | MS.gene47311 | 0.811842 | 5.72E-51 | -1.69E-46 |
| MS.gene060763 | MS.gene47311 | 0.801171 | 1.02E-48 | -1.69E-46 |
| MS.gene060933 | MS.gene47311 | 0.80316 | 3.98E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene47311 | 0.827545 | 1.47E-54 | -1.69E-46 |
| MS.gene060937 | MS.gene47311 | 0.83129 | 1.82E-55 | -1.69E-46 |
| MS.gene061100 | MS.gene47311 | 0.821937 | 3.09E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47311.t1 | MTR_6g027810 | 97.000 | 100 | 3 | 0 | 1 | 100 | 1 | 100 | 6.64e-55 | 166 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47311.t1 | AT1G54410 | 53.247 | 77 | 27 | 2 | 1 | 76 | 1 | 69 | 3.51e-11 | 55.1 |
Find 19 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAAGGATAAGAAGAAGA+AGG | 0.460441 | 6.4:+3159745 | MS.gene47311:CDS |
| GCAGGAATCATCAACAAGAT+TGG | 0.467463 | 6.4:+3159515 | MS.gene47311:CDS |
| GACAAGATCCATGGTGGTGA+AGG | 0.514151 | 6.4:+3159683 | MS.gene47311:CDS |
| AGGTATAGCTGAGAAGATCA+AGG | 0.553688 | 6.4:+3159661 | MS.gene47311:CDS |
| CACAAAGGTGAACACAAAGA+AGG | 0.571597 | 6.4:+3159641 | MS.gene47311:CDS |
| AGTCACAAACAAGTCATGGC+AGG | 0.585630 | 6.4:+3159497 | None:intergenic |
| ATTGGAGAGGCTCTTCATGT+AGG | 0.594022 | 6.4:+3159533 | MS.gene47311:CDS |
| GAGGCTCTTCATGTAGGAGG+AGG | 0.594348 | 6.4:+3159539 | MS.gene47311:CDS |
| ATCCATGGTGGTGAAGGTGA+AGG | 0.602593 | 6.4:+3159689 | MS.gene47311:CDS |
| GGAGAGGCTCTTCATGTAGG+AGG | 0.607476 | 6.4:+3159536 | MS.gene47311:CDS |
| AAGATCAAGGACAAGATCCA+TGG | 0.615180 | 6.4:+3159674 | MS.gene47311:CDS |
| GACCTTCACCTTCACCACCA+TGG | 0.643247 | 6.4:-3159691 | None:intergenic |
| GATAAGAAGAAGAAGGAACA+TGG | 0.650367 | 6.4:+3159752 | MS.gene47311:CDS |
| AAGAAAGAAGATGAACACAA+AGG | 0.654433 | 6.4:+3159566 | MS.gene47311:CDS |
| AATCATCAACAAGATTGGAG+AGG | 0.688291 | 6.4:+3159520 | MS.gene47311:CDS |
| AGTCATGATGACAAGCACAA+AGG | 0.694046 | 6.4:+3159596 | MS.gene47311:CDS |
| AGTCATGATGATAAGCACAA+AGG | 0.702046 | 6.4:+3159626 | MS.gene47311:CDS |
| ATCAAGGACAAGATCCATGG+TGG | 0.715898 | 6.4:+3159677 | MS.gene47311:CDS |
| GGTGAAGGTCATGAACACAA+AGG | 0.734951 | 6.4:+3159704 | MS.gene47311:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GAAGAAAGATAAGAAAAAGA+AGG | + | chr6.4:3159730-3159749 | MS.gene47311:CDS | 25.0% |
| AAAGAAGGATAAGAAGAAGA+AGG | + | chr6.4:3159745-3159764 | MS.gene47311:CDS | 30.0% | |
| AAGAAAGAAGATGAACACAA+AGG | + | chr6.4:3159566-3159585 | MS.gene47311:CDS | 30.0% | |
| AATCATCAACAAGATTGGAG+AGG | + | chr6.4:3159520-3159539 | MS.gene47311:CDS | 35.0% | |
| AGTCATGATGATAAGCACAA+AGG | + | chr6.4:3159626-3159645 | MS.gene47311:CDS | 35.0% | |
| GATAAGAAGAAGAAGGAACA+TGG | + | chr6.4:3159752-3159771 | MS.gene47311:CDS | 35.0% | |
| AAGATCAAGGACAAGATCCA+TGG | + | chr6.4:3159674-3159693 | MS.gene47311:CDS | 40.0% | |
| AGGTATAGCTGAGAAGATCA+AGG | + | chr6.4:3159661-3159680 | MS.gene47311:CDS | 40.0% | |
| AGTCATGATGACAAGCACAA+AGG | + | chr6.4:3159596-3159615 | MS.gene47311:CDS | 40.0% | |
| CACAAAGGTGAACACAAAGA+AGG | + | chr6.4:3159641-3159660 | MS.gene47311:CDS | 40.0% | |
| GCAGGAATCATCAACAAGAT+TGG | + | chr6.4:3159515-3159534 | MS.gene47311:CDS | 40.0% | |
| ATCAAGGACAAGATCCATGG+TGG | + | chr6.4:3159677-3159696 | MS.gene47311:CDS | 45.0% | |
| ATTGGAGAGGCTCTTCATGT+AGG | + | chr6.4:3159533-3159552 | MS.gene47311:CDS | 45.0% | |
| GGTGAAGGTCATGAACACAA+AGG | + | chr6.4:3159704-3159723 | MS.gene47311:CDS | 45.0% | |
| ! | ATCCATGGTGGTGAAGGTGA+AGG | + | chr6.4:3159689-3159708 | MS.gene47311:CDS | 50.0% |
| ! | GACAAGATCCATGGTGGTGA+AGG | + | chr6.4:3159683-3159702 | MS.gene47311:CDS | 50.0% |
| GACCTTCACCTTCACCACCA+TGG | - | chr6.4:3159694-3159713 | None:intergenic | 55.0% | |
| GAGGCTCTTCATGTAGGAGG+AGG | + | chr6.4:3159539-3159558 | MS.gene47311:CDS | 55.0% | |
| GGAGAGGCTCTTCATGTAGG+AGG | + | chr6.4:3159536-3159555 | MS.gene47311:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 3159512 | 3159814 | 3159512 | ID=MS.gene47311 |
| chr6.4 | mRNA | 3159512 | 3159814 | 3159512 | ID=MS.gene47311.t1;Parent=MS.gene47311 |
| chr6.4 | exon | 3159512 | 3159814 | 3159512 | ID=MS.gene47311.t1.exon1;Parent=MS.gene47311.t1 |
| chr6.4 | CDS | 3159512 | 3159814 | 3159512 | ID=cds.MS.gene47311.t1;Parent=MS.gene47311.t1 |
| Gene Sequence |
| Protein sequence |
