Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65518.t1 | XP_013451547.1 | 95.2 | 63 | 3 | 0 | 1 | 63 | 1 | 63 | 3.50E-10 | 74.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65518.t1 | I3SGW9 | 95.2 | 63 | 3 | 0 | 1 | 63 | 1 | 63 | 2.5e-10 | 74.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene65518 | 0.809102 | 2.23E-50 | -1.69E-46 |
| MS.gene049368 | MS.gene65518 | 0.819841 | 9.39E-53 | -1.69E-46 |
| MS.gene049786 | MS.gene65518 | 0.809009 | 2.34E-50 | -1.69E-46 |
| MS.gene050084 | MS.gene65518 | 0.825567 | 4.37E-54 | -1.69E-46 |
| MS.gene050085 | MS.gene65518 | 0.852837 | 3.55E-61 | -1.69E-46 |
| MS.gene050317 | MS.gene65518 | 0.825809 | 3.83E-54 | -1.69E-46 |
| MS.gene050359 | MS.gene65518 | 0.800554 | 1.36E-48 | -1.69E-46 |
| MS.gene050386 | MS.gene65518 | 0.809018 | 2.33E-50 | -1.69E-46 |
| MS.gene050654 | MS.gene65518 | 0.807704 | 4.44E-50 | -1.69E-46 |
| MS.gene050752 | MS.gene65518 | 0.839305 | 1.72E-57 | -1.69E-46 |
| MS.gene050838 | MS.gene65518 | 0.802083 | 6.64E-49 | -1.69E-46 |
| MS.gene050941 | MS.gene65518 | 0.813766 | 2.17E-51 | -1.69E-46 |
| MS.gene051003 | MS.gene65518 | 0.857872 | 1.21E-62 | -1.69E-46 |
| MS.gene051184 | MS.gene65518 | 0.832361 | 9.88E-56 | -1.69E-46 |
| MS.gene051343 | MS.gene65518 | 0.834681 | 2.60E-56 | -1.69E-46 |
| MS.gene051676 | MS.gene65518 | 0.811857 | 5.68E-51 | -1.69E-46 |
| MS.gene051756 | MS.gene65518 | 0.805675 | 1.19E-49 | -1.69E-46 |
| MS.gene051757 | MS.gene65518 | 0.802781 | 4.77E-49 | -1.69E-46 |
| MS.gene051856 | MS.gene65518 | 0.82872 | 7.68E-55 | -1.69E-46 |
| MS.gene051975 | MS.gene65518 | 0.831425 | 1.68E-55 | -1.69E-46 |
| MS.gene052072 | MS.gene65518 | 0.820793 | 5.69E-53 | -1.69E-46 |
| MS.gene052296 | MS.gene65518 | 0.820719 | 5.91E-53 | -1.69E-46 |
| MS.gene052342 | MS.gene65518 | 0.804645 | 1.96E-49 | -1.69E-46 |
| MS.gene052434 | MS.gene65518 | 0.837091 | 6.38E-57 | -1.69E-46 |
| MS.gene053058 | MS.gene65518 | 0.806881 | 6.63E-50 | -1.69E-46 |
| MS.gene053295 | MS.gene65518 | 0.821607 | 3.69E-53 | -1.69E-46 |
| MS.gene053564 | MS.gene65518 | 0.806247 | 9.02E-50 | -1.69E-46 |
| MS.gene053590 | MS.gene65518 | 0.828859 | 7.11E-55 | -1.69E-46 |
| MS.gene053931 | MS.gene65518 | 0.804049 | 2.60E-49 | -1.69E-46 |
| MS.gene054403 | MS.gene65518 | 0.836006 | 1.20E-56 | -1.69E-46 |
| MS.gene05469 | MS.gene65518 | 0.836902 | 7.13E-57 | -1.69E-46 |
| MS.gene05517 | MS.gene65518 | 0.814602 | 1.42E-51 | -1.69E-46 |
| MS.gene055311 | MS.gene65518 | 0.8213 | 4.35E-53 | -1.69E-46 |
| MS.gene055313 | MS.gene65518 | 0.805034 | 1.62E-49 | -1.69E-46 |
| MS.gene055441 | MS.gene65518 | 0.801528 | 8.63E-49 | -1.69E-46 |
| MS.gene056181 | MS.gene65518 | 0.846412 | 2.21E-59 | -1.69E-46 |
| MS.gene056787 | MS.gene65518 | 0.853097 | 2.99E-61 | -1.69E-46 |
| MS.gene056986 | MS.gene65518 | 0.833724 | 4.53E-56 | -1.69E-46 |
| MS.gene057140 | MS.gene65518 | 0.80212 | 6.52E-49 | -1.69E-46 |
| MS.gene057234 | MS.gene65518 | 0.8336 | 4.86E-56 | -1.69E-46 |
| MS.gene05781 | MS.gene65518 | 0.815195 | 1.05E-51 | -1.69E-46 |
| MS.gene057890 | MS.gene65518 | -0.808717 | 2.70E-50 | -1.69E-46 |
| MS.gene057999 | MS.gene65518 | 0.823407 | 1.41E-53 | -1.69E-46 |
| MS.gene058054 | MS.gene65518 | 0.839497 | 1.53E-57 | -1.69E-46 |
| MS.gene058055 | MS.gene65518 | 0.832669 | 8.29E-56 | -1.69E-46 |
| MS.gene058159 | MS.gene65518 | 0.803306 | 3.71E-49 | -1.69E-46 |
| MS.gene058238 | MS.gene65518 | 0.800853 | 1.19E-48 | -1.69E-46 |
| MS.gene058292 | MS.gene65518 | 0.82332 | 1.48E-53 | -1.69E-46 |
| MS.gene058646 | MS.gene65518 | 0.801592 | 8.37E-49 | -1.69E-46 |
| MS.gene058654 | MS.gene65518 | 0.80007 | 1.71E-48 | -1.69E-46 |
| MS.gene058852 | MS.gene65518 | 0.802014 | 6.86E-49 | -1.69E-46 |
| MS.gene059050 | MS.gene65518 | 0.825742 | 3.97E-54 | -1.69E-46 |
| MS.gene059146 | MS.gene65518 | 0.839185 | 1.84E-57 | -1.69E-46 |
| MS.gene059227 | MS.gene65518 | 0.801746 | 7.79E-49 | -1.69E-46 |
| MS.gene059360 | MS.gene65518 | 0.820146 | 8.00E-53 | -1.69E-46 |
| MS.gene06010 | MS.gene65518 | 0.845555 | 3.78E-59 | -1.69E-46 |
| MS.gene06011 | MS.gene65518 | 0.83272 | 8.05E-56 | -1.69E-46 |
| MS.gene060702 | MS.gene65518 | 0.816394 | 5.66E-52 | -1.69E-46 |
| MS.gene060763 | MS.gene65518 | 0.822374 | 2.45E-53 | -1.69E-46 |
| MS.gene060869 | MS.gene65518 | 0.807852 | 4.13E-50 | -1.69E-46 |
| MS.gene060870 | MS.gene65518 | 0.828274 | 9.85E-55 | -1.69E-46 |
| MS.gene060935 | MS.gene65518 | 0.815461 | 9.13E-52 | -1.69E-46 |
| MS.gene060937 | MS.gene65518 | 0.819368 | 1.20E-52 | -1.69E-46 |
| MS.gene061100 | MS.gene65518 | 0.805692 | 1.18E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65518.t1 | MTR_6g027810 | 97.000 | 100 | 3 | 0 | 1 | 100 | 1 | 100 | 6.64e-55 | 166 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65518.t1 | AT1G54410 | 53.247 | 77 | 27 | 2 | 1 | 76 | 1 | 69 | 3.51e-11 | 55.1 |
Find 19 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAAGGATAAGAAGAAGA+AGG | 0.460441 | 6.3:+19794668 | MS.gene65518:CDS |
| GCAGGAATCATCAACAAGAT+TGG | 0.467463 | 6.3:+19794438 | MS.gene65518:CDS |
| GACAAGATCCATGGTGGTGA+AGG | 0.514151 | 6.3:+19794606 | MS.gene65518:CDS |
| AGGTATAGCTGAGAAGATCA+AGG | 0.553688 | 6.3:+19794584 | MS.gene65518:CDS |
| CACAAAGGTGAACACAAAGA+AGG | 0.571597 | 6.3:+19794564 | MS.gene65518:CDS |
| AGTCACAAACAAGTCATGGC+AGG | 0.585630 | 6.3:+19794420 | None:intergenic |
| ATTGGAGAGGCTCTTCATGT+AGG | 0.594022 | 6.3:+19794456 | MS.gene65518:CDS |
| GAGGCTCTTCATGTAGGAGG+AGG | 0.594348 | 6.3:+19794462 | MS.gene65518:CDS |
| ATCCATGGTGGTGAAGGTGA+AGG | 0.602593 | 6.3:+19794612 | MS.gene65518:CDS |
| GGAGAGGCTCTTCATGTAGG+AGG | 0.607476 | 6.3:+19794459 | MS.gene65518:CDS |
| AAGATCAAGGACAAGATCCA+TGG | 0.615180 | 6.3:+19794597 | MS.gene65518:CDS |
| GACCTTCACCTTCACCACCA+TGG | 0.643247 | 6.3:-19794614 | None:intergenic |
| GATAAGAAGAAGAAGGAACA+TGG | 0.650367 | 6.3:+19794675 | MS.gene65518:CDS |
| AAGAAAGAAGATGAACACAA+AGG | 0.654433 | 6.3:+19794489 | MS.gene65518:CDS |
| AATCATCAACAAGATTGGAG+AGG | 0.688291 | 6.3:+19794443 | MS.gene65518:CDS |
| AGTCATGATGACAAGCACAA+AGG | 0.694046 | 6.3:+19794519 | MS.gene65518:CDS |
| AGTCATGATGATAAGCACAA+AGG | 0.702046 | 6.3:+19794549 | MS.gene65518:CDS |
| ATCAAGGACAAGATCCATGG+TGG | 0.715898 | 6.3:+19794600 | MS.gene65518:CDS |
| GGTGAAGGTCATGAACACAA+AGG | 0.734951 | 6.3:+19794627 | MS.gene65518:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GAAGAAAGATAAGAAAAAGA+AGG | + | chr6.3:19794653-19794672 | MS.gene65518:CDS | 25.0% |
| AAAGAAGGATAAGAAGAAGA+AGG | + | chr6.3:19794668-19794687 | MS.gene65518:CDS | 30.0% | |
| AAGAAAGAAGATGAACACAA+AGG | + | chr6.3:19794489-19794508 | MS.gene65518:CDS | 30.0% | |
| AATCATCAACAAGATTGGAG+AGG | + | chr6.3:19794443-19794462 | MS.gene65518:CDS | 35.0% | |
| AGTCATGATGATAAGCACAA+AGG | + | chr6.3:19794549-19794568 | MS.gene65518:CDS | 35.0% | |
| GATAAGAAGAAGAAGGAACA+TGG | + | chr6.3:19794675-19794694 | MS.gene65518:CDS | 35.0% | |
| AAGATCAAGGACAAGATCCA+TGG | + | chr6.3:19794597-19794616 | MS.gene65518:CDS | 40.0% | |
| AGGTATAGCTGAGAAGATCA+AGG | + | chr6.3:19794584-19794603 | MS.gene65518:CDS | 40.0% | |
| AGTCATGATGACAAGCACAA+AGG | + | chr6.3:19794519-19794538 | MS.gene65518:CDS | 40.0% | |
| CACAAAGGTGAACACAAAGA+AGG | + | chr6.3:19794564-19794583 | MS.gene65518:CDS | 40.0% | |
| GCAGGAATCATCAACAAGAT+TGG | + | chr6.3:19794438-19794457 | MS.gene65518:CDS | 40.0% | |
| ATCAAGGACAAGATCCATGG+TGG | + | chr6.3:19794600-19794619 | MS.gene65518:CDS | 45.0% | |
| ATTGGAGAGGCTCTTCATGT+AGG | + | chr6.3:19794456-19794475 | MS.gene65518:CDS | 45.0% | |
| GGTGAAGGTCATGAACACAA+AGG | + | chr6.3:19794627-19794646 | MS.gene65518:CDS | 45.0% | |
| ! | ATCCATGGTGGTGAAGGTGA+AGG | + | chr6.3:19794612-19794631 | MS.gene65518:CDS | 50.0% |
| ! | GACAAGATCCATGGTGGTGA+AGG | + | chr6.3:19794606-19794625 | MS.gene65518:CDS | 50.0% |
| GACCTTCACCTTCACCACCA+TGG | - | chr6.3:19794617-19794636 | None:intergenic | 55.0% | |
| GAGGCTCTTCATGTAGGAGG+AGG | + | chr6.3:19794462-19794481 | MS.gene65518:CDS | 55.0% | |
| GGAGAGGCTCTTCATGTAGG+AGG | + | chr6.3:19794459-19794478 | MS.gene65518:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 19794435 | 19794737 | 19794435 | ID=MS.gene65518 |
| chr6.3 | mRNA | 19794435 | 19794737 | 19794435 | ID=MS.gene65518.t1;Parent=MS.gene65518 |
| chr6.3 | exon | 19794435 | 19794737 | 19794435 | ID=MS.gene65518.t1.exon1;Parent=MS.gene65518.t1 |
| chr6.3 | CDS | 19794435 | 19794737 | 19794435 | ID=cds.MS.gene65518.t1;Parent=MS.gene65518.t1 |
| Gene Sequence |
| Protein sequence |
