Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77361.t1 | XP_013449157.1 | 97.7 | 176 | 3 | 1 | 1 | 175 | 118 | 293 | 1.40E-30 | 142.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77361.t1 | Q9SDW0 | 52.6 | 196 | 69 | 1 | 2 | 173 | 118 | 313 | 1.2e-09 | 64.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77361.t1 | A0A072U082 | 97.7 | 176 | 3 | 1 | 1 | 175 | 118 | 293 | 1.0e-30 | 142.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene77361 | 0.816226 | 6.17E-52 | -1.69E-46 |
| MS.gene04918 | MS.gene77361 | 0.868156 | 8.05E-66 | -1.69E-46 |
| MS.gene04919 | MS.gene77361 | 0.854704 | 1.03E-61 | -1.69E-46 |
| MS.gene04920 | MS.gene77361 | 0.873444 | 1.46E-67 | -1.69E-46 |
| MS.gene049254 | MS.gene77361 | 0.817808 | 2.72E-52 | -1.69E-46 |
| MS.gene049283 | MS.gene77361 | 0.865558 | 5.41E-65 | -1.69E-46 |
| MS.gene049368 | MS.gene77361 | 0.842011 | 3.35E-58 | -1.69E-46 |
| MS.gene049419 | MS.gene77361 | -0.840658 | 7.62E-58 | -1.69E-46 |
| MS.gene049452 | MS.gene77361 | 0.821897 | 3.16E-53 | -1.69E-46 |
| MS.gene049499 | MS.gene77361 | 0.876626 | 1.20E-68 | -1.69E-46 |
| MS.gene049680 | MS.gene77361 | 0.804447 | 2.15E-49 | -1.69E-46 |
| MS.gene049983 | MS.gene77361 | 0.889406 | 2.49E-73 | -1.69E-46 |
| MS.gene04998 | MS.gene77361 | 0.803723 | 3.04E-49 | -1.69E-46 |
| MS.gene050359 | MS.gene77361 | 0.811217 | 7.82E-51 | -1.69E-46 |
| MS.gene050414 | MS.gene77361 | 0.850463 | 1.67E-60 | -1.69E-46 |
| MS.gene050571 | MS.gene77361 | 0.805844 | 1.10E-49 | -1.69E-46 |
| MS.gene050646 | MS.gene77361 | 0.854784 | 9.77E-62 | -1.69E-46 |
| MS.gene050654 | MS.gene77361 | 0.871345 | 7.34E-67 | -1.69E-46 |
| MS.gene050737 | MS.gene77361 | 0.828445 | 8.95E-55 | -1.69E-46 |
| MS.gene050941 | MS.gene77361 | 0.852744 | 3.78E-61 | -1.69E-46 |
| MS.gene050945 | MS.gene77361 | 0.800541 | 1.37E-48 | -1.69E-46 |
| MS.gene051003 | MS.gene77361 | 0.835008 | 2.15E-56 | -1.69E-46 |
| MS.gene051043 | MS.gene77361 | 0.821189 | 4.61E-53 | -1.69E-46 |
| MS.gene051184 | MS.gene77361 | 0.821304 | 4.33E-53 | -1.69E-46 |
| MS.gene051234 | MS.gene77361 | 0.830717 | 2.51E-55 | -1.69E-46 |
| MS.gene051275 | MS.gene77361 | 0.832068 | 1.17E-55 | -1.69E-46 |
| MS.gene051332 | MS.gene77361 | 0.806533 | 7.86E-50 | -1.69E-46 |
| MS.gene051343 | MS.gene77361 | 0.810269 | 1.25E-50 | -1.69E-46 |
| MS.gene051363 | MS.gene77361 | 0.805799 | 1.12E-49 | -1.69E-46 |
| MS.gene051365 | MS.gene77361 | 0.81267 | 3.77E-51 | -1.69E-46 |
| MS.gene051541 | MS.gene77361 | 0.828393 | 9.22E-55 | -1.69E-46 |
| MS.gene051676 | MS.gene77361 | 0.822503 | 2.29E-53 | -1.69E-46 |
| MS.gene051831 | MS.gene77361 | 0.815274 | 1.01E-51 | -1.69E-46 |
| MS.gene051852 | MS.gene77361 | 0.817271 | 3.60E-52 | -1.69E-46 |
| MS.gene051856 | MS.gene77361 | 0.808621 | 2.83E-50 | -1.69E-46 |
| MS.gene05188 | MS.gene77361 | 0.805052 | 1.61E-49 | -1.69E-46 |
| MS.gene051902 | MS.gene77361 | 0.806066 | 9.85E-50 | -1.69E-46 |
| MS.gene051975 | MS.gene77361 | 0.825006 | 5.93E-54 | -1.69E-46 |
| MS.gene052016 | MS.gene77361 | 0.837072 | 6.45E-57 | -1.69E-46 |
| MS.gene052072 | MS.gene77361 | 0.817682 | 2.91E-52 | -1.69E-46 |
| MS.gene052118 | MS.gene77361 | 0.830648 | 2.61E-55 | -1.69E-46 |
| MS.gene052296 | MS.gene77361 | 0.83676 | 7.75E-57 | -1.69E-46 |
| MS.gene052307 | MS.gene77361 | 0.820013 | 8.58E-53 | -1.69E-46 |
| MS.gene052342 | MS.gene77361 | 0.839667 | 1.38E-57 | -1.69E-46 |
| MS.gene052434 | MS.gene77361 | 0.806401 | 8.38E-50 | -1.69E-46 |
| MS.gene052463 | MS.gene77361 | 0.847858 | 8.86E-60 | -1.69E-46 |
| MS.gene052518 | MS.gene77361 | 0.851929 | 6.44E-61 | -1.69E-46 |
| MS.gene052533 | MS.gene77361 | 0.828186 | 1.03E-54 | -1.69E-46 |
| MS.gene052534 | MS.gene77361 | 0.817607 | 3.02E-52 | -1.69E-46 |
| MS.gene052674 | MS.gene77361 | 0.813338 | 2.69E-51 | -1.69E-46 |
| MS.gene052675 | MS.gene77361 | 0.805169 | 1.52E-49 | -1.69E-46 |
| MS.gene052677 | MS.gene77361 | 0.808308 | 3.30E-50 | -1.69E-46 |
| MS.gene052685 | MS.gene77361 | 0.810353 | 1.20E-50 | -1.69E-46 |
| MS.gene052793 | MS.gene77361 | 0.812026 | 5.21E-51 | -1.69E-46 |
| MS.gene052855 | MS.gene77361 | 0.804163 | 2.47E-49 | -1.69E-46 |
| MS.gene052932 | MS.gene77361 | 0.813152 | 2.96E-51 | -1.69E-46 |
| MS.gene053058 | MS.gene77361 | 0.806462 | 8.13E-50 | -1.69E-46 |
| MS.gene053228 | MS.gene77361 | 0.808132 | 3.60E-50 | -1.69E-46 |
| MS.gene053272 | MS.gene77361 | -0.809231 | 2.10E-50 | -1.69E-46 |
| MS.gene053295 | MS.gene77361 | 0.82172 | 3.47E-53 | -1.69E-46 |
| MS.gene053298 | MS.gene77361 | 0.841889 | 3.61E-58 | -1.69E-46 |
| MS.gene053456 | MS.gene77361 | 0.838707 | 2.45E-57 | -1.69E-46 |
| MS.gene05350 | MS.gene77361 | 0.808436 | 3.10E-50 | -1.69E-46 |
| MS.gene053564 | MS.gene77361 | 0.815457 | 9.15E-52 | -1.69E-46 |
| MS.gene053726 | MS.gene77361 | 0.836557 | 8.72E-57 | -1.69E-46 |
| MS.gene053786 | MS.gene77361 | 0.812073 | 5.09E-51 | -1.69E-46 |
| MS.gene053879 | MS.gene77361 | 0.828969 | 6.69E-55 | -1.69E-46 |
| MS.gene053931 | MS.gene77361 | 0.829914 | 3.94E-55 | -1.69E-46 |
| MS.gene053963 | MS.gene77361 | 0.828191 | 1.03E-54 | -1.69E-46 |
| MS.gene054004 | MS.gene77361 | 0.825889 | 3.66E-54 | -1.69E-46 |
| MS.gene054178 | MS.gene77361 | 0.813565 | 2.40E-51 | -1.69E-46 |
| MS.gene054222 | MS.gene77361 | 0.803021 | 4.25E-49 | -1.69E-46 |
| MS.gene05423 | MS.gene77361 | 0.801621 | 8.26E-49 | -1.69E-46 |
| MS.gene054479 | MS.gene77361 | 0.831326 | 1.78E-55 | -1.69E-46 |
| MS.gene054489 | MS.gene77361 | 0.817254 | 3.63E-52 | -1.69E-46 |
| MS.gene05458 | MS.gene77361 | 0.846557 | 2.02E-59 | -1.69E-46 |
| MS.gene054641 | MS.gene77361 | 0.801501 | 8.74E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene77361 | 0.806222 | 9.13E-50 | -1.69E-46 |
| MS.gene05469 | MS.gene77361 | 0.801515 | 8.68E-49 | -1.69E-46 |
| MS.gene054772 | MS.gene77361 | 0.822354 | 2.48E-53 | -1.69E-46 |
| MS.gene054892 | MS.gene77361 | 0.863964 | 1.71E-64 | -1.69E-46 |
| MS.gene054893 | MS.gene77361 | 0.870084 | 1.91E-66 | -1.69E-46 |
| MS.gene054957 | MS.gene77361 | 0.805992 | 1.02E-49 | -1.69E-46 |
| MS.gene055150 | MS.gene77361 | 0.822629 | 2.14E-53 | -1.69E-46 |
| MS.gene05517 | MS.gene77361 | 0.815822 | 7.59E-52 | -1.69E-46 |
| MS.gene055513 | MS.gene77361 | 0.842886 | 1.97E-58 | -1.69E-46 |
| MS.gene05552 | MS.gene77361 | 0.804865 | 1.76E-49 | -1.69E-46 |
| MS.gene055724 | MS.gene77361 | 0.804353 | 2.25E-49 | -1.69E-46 |
| MS.gene055801 | MS.gene77361 | 0.800424 | 1.45E-48 | -1.69E-46 |
| MS.gene055841 | MS.gene77361 | 0.805376 | 1.38E-49 | -1.69E-46 |
| MS.gene055864 | MS.gene77361 | 0.815879 | 7.37E-52 | -1.69E-46 |
| MS.gene056045 | MS.gene77361 | 0.829651 | 4.57E-55 | -1.69E-46 |
| MS.gene056082 | MS.gene77361 | 0.812117 | 4.98E-51 | -1.69E-46 |
| MS.gene056181 | MS.gene77361 | 0.81632 | 5.88E-52 | -1.69E-46 |
| MS.gene056699 | MS.gene77361 | 0.848694 | 5.20E-60 | -1.69E-46 |
| MS.gene056776 | MS.gene77361 | 0.810923 | 9.06E-51 | -1.69E-46 |
| MS.gene056825 | MS.gene77361 | 0.807204 | 5.67E-50 | -1.69E-46 |
| MS.gene056831 | MS.gene77361 | 0.813605 | 2.35E-51 | -1.69E-46 |
| MS.gene056834 | MS.gene77361 | 0.812945 | 3.28E-51 | -1.69E-46 |
| MS.gene05686 | MS.gene77361 | 0.831792 | 1.37E-55 | -1.69E-46 |
| MS.gene05690 | MS.gene77361 | 0.809463 | 1.87E-50 | -1.69E-46 |
| MS.gene056973 | MS.gene77361 | 0.800732 | 1.26E-48 | -1.69E-46 |
| MS.gene056986 | MS.gene77361 | 0.807395 | 5.16E-50 | -1.69E-46 |
| MS.gene057102 | MS.gene77361 | 0.850323 | 1.83E-60 | -1.69E-46 |
| MS.gene057155 | MS.gene77361 | 0.823989 | 1.03E-53 | -1.69E-46 |
| MS.gene057208 | MS.gene77361 | 0.847061 | 1.47E-59 | -1.69E-46 |
| MS.gene057371 | MS.gene77361 | 0.833089 | 6.51E-56 | -1.69E-46 |
| MS.gene057622 | MS.gene77361 | 0.852557 | 4.27E-61 | -1.69E-46 |
| MS.gene057890 | MS.gene77361 | -0.815247 | 1.02E-51 | -1.69E-46 |
| MS.gene057999 | MS.gene77361 | 0.801583 | 8.41E-49 | -1.69E-46 |
| MS.gene058081 | MS.gene77361 | 0.814351 | 1.61E-51 | -1.69E-46 |
| MS.gene058095 | MS.gene77361 | 0.815935 | 7.17E-52 | -1.69E-46 |
| MS.gene058159 | MS.gene77361 | 0.860583 | 1.87E-63 | -1.69E-46 |
| MS.gene058261 | MS.gene77361 | 0.804926 | 1.71E-49 | -1.69E-46 |
| MS.gene058279 | MS.gene77361 | 0.82078 | 5.73E-53 | -1.69E-46 |
| MS.gene058292 | MS.gene77361 | 0.808407 | 3.14E-50 | -1.69E-46 |
| MS.gene058429 | MS.gene77361 | 0.861433 | 1.03E-63 | -1.69E-46 |
| MS.gene058592 | MS.gene77361 | 0.830632 | 2.63E-55 | -1.69E-46 |
| MS.gene058781 | MS.gene77361 | 0.851787 | 7.07E-61 | -1.69E-46 |
| MS.gene058783 | MS.gene77361 | 0.837813 | 4.17E-57 | -1.69E-46 |
| MS.gene05878 | MS.gene77361 | 0.816933 | 4.28E-52 | -1.69E-46 |
| MS.gene059146 | MS.gene77361 | 0.854096 | 1.55E-61 | -1.69E-46 |
| MS.gene059170 | MS.gene77361 | 0.853807 | 1.87E-61 | -1.69E-46 |
| MS.gene059283 | MS.gene77361 | 0.806054 | 9.91E-50 | -1.69E-46 |
| MS.gene05952 | MS.gene77361 | 0.8696 | 2.74E-66 | -1.69E-46 |
| MS.gene05985 | MS.gene77361 | -0.846239 | 2.46E-59 | -1.69E-46 |
| MS.gene059901 | MS.gene77361 | -0.820334 | 7.24E-53 | -1.69E-46 |
| MS.gene06010 | MS.gene77361 | 0.821647 | 3.61E-53 | -1.69E-46 |
| MS.gene06011 | MS.gene77361 | 0.821095 | 4.84E-53 | -1.69E-46 |
| MS.gene060244 | MS.gene77361 | 0.827224 | 1.76E-54 | -1.69E-46 |
| MS.gene060259 | MS.gene77361 | 0.861216 | 1.20E-63 | -1.69E-46 |
| MS.gene060365 | MS.gene77361 | 0.816631 | 5.01E-52 | -1.69E-46 |
| MS.gene060412 | MS.gene77361 | 0.802811 | 4.70E-49 | -1.69E-46 |
| MS.gene060435 | MS.gene77361 | 0.823474 | 1.36E-53 | -1.69E-46 |
| MS.gene060522 | MS.gene77361 | 0.810442 | 1.15E-50 | -1.69E-46 |
| MS.gene060527 | MS.gene77361 | 0.833143 | 6.32E-56 | -1.69E-46 |
| MS.gene060763 | MS.gene77361 | 0.807859 | 4.12E-50 | -1.69E-46 |
| MS.gene060777 | MS.gene77361 | 0.821207 | 4.57E-53 | -1.69E-46 |
| MS.gene060785 | MS.gene77361 | 0.807988 | 3.86E-50 | -1.69E-46 |
| MS.gene060850 | MS.gene77361 | 0.809709 | 1.65E-50 | -1.69E-46 |
| MS.gene060851 | MS.gene77361 | 0.814074 | 1.85E-51 | -1.69E-46 |
| MS.gene060852 | MS.gene77361 | 0.813557 | 2.41E-51 | -1.69E-46 |
| MS.gene060932 | MS.gene77361 | 0.866727 | 2.31E-65 | -1.69E-46 |
| MS.gene060933 | MS.gene77361 | 0.839548 | 1.48E-57 | -1.69E-46 |
| MS.gene060935 | MS.gene77361 | 0.852123 | 5.68E-61 | -1.69E-46 |
| MS.gene060937 | MS.gene77361 | 0.867751 | 1.09E-65 | -1.69E-46 |
| MS.gene061100 | MS.gene77361 | 0.861524 | 9.66E-64 | -1.69E-46 |
| MS.gene061160 | MS.gene77361 | 0.831863 | 1.31E-55 | -1.69E-46 |
| MS.gene061188 | MS.gene77361 | 0.845112 | 4.98E-59 | -1.69E-46 |
| MS.gene06121 | MS.gene77361 | 0.822349 | 2.48E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77361.t1 | MTR_7g068770 | 97.727 | 176 | 3 | 1 | 1 | 175 | 118 | 293 | 3.83e-119 | 338 |
| MS.gene77361.t1 | MTR_4g015680 | 71.676 | 173 | 49 | 0 | 1 | 173 | 107 | 279 | 7.87e-61 | 190 |
| MS.gene77361.t1 | MTR_5g026540 | 42.473 | 186 | 87 | 6 | 8 | 174 | 117 | 301 | 1.97e-21 | 89.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77361.t1 | AT5G01380 | 47.959 | 196 | 78 | 3 | 2 | 173 | 118 | 313 | 7.37e-32 | 117 |
| MS.gene77361.t1 | AT2G38250 | 52.151 | 186 | 70 | 3 | 1 | 173 | 107 | 286 | 8.77e-27 | 102 |
Find 43 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTGAAGCTCATTGTAAAA+TGG | 0.335464 | 7.2:+34961517 | None:intergenic |
| TGAGAGGAGATTGAAGGAGA+TGG | 0.403012 | 7.2:-34961206 | MS.gene77361:CDS |
| TGGAACAAAGATGGAGAGAA+AGG | 0.438127 | 7.2:-34961138 | MS.gene77361:CDS |
| GGAGATTGAAGGAGATGGAA+TGG | 0.449920 | 7.2:-34961201 | MS.gene77361:CDS |
| GAAGGATTAGAGAGGAAGCT+AGG | 0.463838 | 7.2:-34961105 | MS.gene77361:CDS |
| AGATTCTTGAGGAGTTTATG+AGG | 0.475642 | 7.2:-34961282 | MS.gene77361:CDS |
| TCAATGGATGGAAGCATTTG+AGG | 0.478413 | 7.2:-34961239 | MS.gene77361:CDS |
| TCTGAGGATGAAGAAGAGTT+AGG | 0.481694 | 7.2:-34961415 | MS.gene77361:CDS |
| AAGGAGGGTCAAAGAAGAAA+GGG | 0.488338 | 7.2:-34961450 | MS.gene77361:CDS |
| GGAGAATGAGAGGAGATTGA+AGG | 0.494385 | 7.2:-34961212 | MS.gene77361:CDS |
| AAATGCAAATGGAAGCTCAA+TGG | 0.497271 | 7.2:-34961255 | MS.gene77361:CDS |
| GATGGAATGGAGACAAAGAA+TGG | 0.497428 | 7.2:-34961188 | MS.gene77361:CDS |
| TATGAGGCAACAAATGCAAA+TGG | 0.498234 | 7.2:-34961266 | MS.gene77361:CDS |
| GGTTAATGATGGAACAAAGA+TGG | 0.500019 | 7.2:-34961147 | MS.gene77361:CDS |
| GTTAGGCAATGAAGAAAGTG+AGG | 0.500463 | 7.2:-34961398 | MS.gene77361:CDS |
| GAGACAAAGAATGGAAGCAT+TGG | 0.501965 | 7.2:-34961179 | MS.gene77361:CDS |
| GAAGGAGGGTCAAAGAAGAA+AGG | 0.513151 | 7.2:-34961451 | MS.gene77361:CDS |
| AAAGGTAAAATGATAATTGG+TGG | 0.519046 | 7.2:-34961340 | MS.gene77361:CDS |
| CAAGGATGCAAAGAATGCTA+TGG | 0.520600 | 7.2:-34961483 | MS.gene77361:CDS |
| GAGAAAGGGAAGAACAAAGA+AGG | 0.520747 | 7.2:-34961123 | MS.gene77361:CDS |
| GGAACAAAGATGGAGAGAAA+GGG | 0.522554 | 7.2:-34961137 | MS.gene77361:CDS |
| TAGTGGTAGCAACAATTTGA+AGG | 0.523300 | 7.2:-34961305 | MS.gene77361:CDS |
| GGTGGAGGAAATGATAATAG+TGG | 0.531218 | 7.2:-34961322 | MS.gene77361:CDS |
| TGGAAGCATTGGAGAATGAG+AGG | 0.532379 | 7.2:-34961168 | MS.gene77361:CDS |
| GGAGAATGAGAGGTTAATGA+TGG | 0.535043 | 7.2:-34961158 | MS.gene77361:CDS |
| CAATTTGAAGGAGATTCTTG+AGG | 0.551112 | 7.2:-34961293 | MS.gene77361:CDS |
| GGTAAAATGATAATTGGTGG+AGG | 0.559022 | 7.2:-34961337 | MS.gene77361:CDS |
| AAATGGAAATTGTTGTCTCA+TGG | 0.560448 | 7.2:+34961534 | None:intergenic |
| AAGGATTAGAGAGGAAGCTA+GGG | 0.562239 | 7.2:-34961104 | MS.gene77361:CDS |
| ATGCTATGGGCTGAAGCAGA+AGG | 0.573045 | 7.2:-34961469 | MS.gene77361:CDS |
| GGATGGAAGCATTTGAGGCA+AGG | 0.573712 | 7.2:-34961234 | MS.gene77361:CDS |
| TTGAGGCAAGGGAGAATGAG+AGG | 0.584023 | 7.2:-34961222 | MS.gene77361:CDS |
| GCAAATGGAAGCTCAATGGA+TGG | 0.584747 | 7.2:-34961251 | MS.gene77361:CDS |
| GATGGAAGCATTTGAGGCAA+GGG | 0.587229 | 7.2:-34961233 | MS.gene77361:CDS |
| AGAAAGTGAGGGAGATCACA+AGG | 0.594638 | 7.2:-34961386 | MS.gene77361:CDS |
| TTAGGCAATGAAGAAAGTGA+GGG | 0.597635 | 7.2:-34961397 | MS.gene77361:CDS |
| CTATGGGCTGAAGCAGAAGG+AGG | 0.599474 | 7.2:-34961466 | MS.gene77361:CDS |
| AGAACAAAGAAGGATTAGAG+AGG | 0.604574 | 7.2:-34961113 | MS.gene77361:CDS |
| AGGGGTGCATGTATCTTCTG+AGG | 0.631436 | 7.2:-34961431 | MS.gene77361:CDS |
| AAGGATGCAAAGAATGCTAT+GGG | 0.642173 | 7.2:-34961482 | MS.gene77361:CDS |
| AGGAGGGTCAAAGAAGAAAG+GGG | 0.670389 | 7.2:-34961449 | MS.gene77361:CDS |
| TATGGGCTGAAGCAGAAGGA+GGG | 0.673793 | 7.2:-34961465 | MS.gene77361:CDS |
| GAAAGTGAGGGAGATCACAA+GGG | 0.730407 | 7.2:-34961385 | MS.gene77361:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAGGTAAAATGATAAT+TGG | - | chr7.2:34961246-34961265 | MS.gene77361:CDS | 15.0% |
| !! | ATTAAAAAGAAGAAGAAAAA+AGG | - | chr7.2:34961231-34961250 | MS.gene77361:CDS | 15.0% |
| ! | AAAGGTAAAATGATAATTGG+TGG | - | chr7.2:34961249-34961268 | MS.gene77361:CDS | 25.0% |
| AAATGGAAATTGTTGTCTCA+TGG | + | chr7.2:34961058-34961077 | None:intergenic | 30.0% | |
| !!! | TTCAAGCTATTTTTAGTGCA+AGG | - | chr7.2:34961088-34961107 | MS.gene77361:CDS | 30.0% |
| AAATGCAAATGGAAGCTCAA+TGG | - | chr7.2:34961334-34961353 | MS.gene77361:CDS | 35.0% | |
| AAGGATGCAAAGAATGCTAT+GGG | - | chr7.2:34961107-34961126 | MS.gene77361:CDS | 35.0% | |
| AGAACAAAGAAGGATTAGAG+AGG | - | chr7.2:34961476-34961495 | MS.gene77361:CDS | 35.0% | |
| AGATTCTTGAGGAGTTTATG+AGG | - | chr7.2:34961307-34961326 | MS.gene77361:CDS | 35.0% | |
| CAATTTGAAGGAGATTCTTG+AGG | - | chr7.2:34961296-34961315 | MS.gene77361:CDS | 35.0% | |
| GCTTGAAGCTCATTGTAAAA+TGG | + | chr7.2:34961075-34961094 | None:intergenic | 35.0% | |
| GGTAAAATGATAATTGGTGG+AGG | - | chr7.2:34961252-34961271 | MS.gene77361:CDS | 35.0% | |
| TAGTGGTAGCAACAATTTGA+AGG | - | chr7.2:34961284-34961303 | MS.gene77361:CDS | 35.0% | |
| TATGAGGCAACAAATGCAAA+TGG | - | chr7.2:34961323-34961342 | MS.gene77361:CDS | 35.0% | |
| TTAGGCAATGAAGAAAGTGA+GGG | - | chr7.2:34961192-34961211 | MS.gene77361:CDS | 35.0% | |
| ! | GGTTAATGATGGAACAAAGA+TGG | - | chr7.2:34961442-34961461 | MS.gene77361:CDS | 35.0% |
| AAGGAGGGTCAAAGAAGAAA+GGG | - | chr7.2:34961139-34961158 | MS.gene77361:CDS | 40.0% | |
| AAGGATTAGAGAGGAAGCTA+GGG | - | chr7.2:34961485-34961504 | MS.gene77361:CDS | 40.0% | |
| CAAGGATGCAAAGAATGCTA+TGG | - | chr7.2:34961106-34961125 | MS.gene77361:CDS | 40.0% | |
| GAGAAAGGGAAGAACAAAGA+AGG | - | chr7.2:34961466-34961485 | MS.gene77361:CDS | 40.0% | |
| GATGGAATGGAGACAAAGAA+TGG | - | chr7.2:34961401-34961420 | MS.gene77361:CDS | 40.0% | |
| GGAACAAAGATGGAGAGAAA+GGG | - | chr7.2:34961452-34961471 | MS.gene77361:CDS | 40.0% | |
| GGAGAATGAGAGGTTAATGA+TGG | - | chr7.2:34961431-34961450 | MS.gene77361:CDS | 40.0% | |
| GGTGGAGGAAATGATAATAG+TGG | - | chr7.2:34961267-34961286 | MS.gene77361:CDS | 40.0% | |
| GTTAGGCAATGAAGAAAGTG+AGG | - | chr7.2:34961191-34961210 | MS.gene77361:CDS | 40.0% | |
| TCTGAGGATGAAGAAGAGTT+AGG | - | chr7.2:34961174-34961193 | MS.gene77361:CDS | 40.0% | |
| TGGAACAAAGATGGAGAGAA+AGG | - | chr7.2:34961451-34961470 | MS.gene77361:CDS | 40.0% | |
| ! | GAGACAAAGAATGGAAGCAT+TGG | - | chr7.2:34961410-34961429 | MS.gene77361:CDS | 40.0% |
| !! | TCAATGGATGGAAGCATTTG+AGG | - | chr7.2:34961350-34961369 | MS.gene77361:CDS | 40.0% |
| AGAAAGTGAGGGAGATCACA+AGG | - | chr7.2:34961203-34961222 | MS.gene77361:CDS | 45.0% | |
| AGGAGGGTCAAAGAAGAAAG+GGG | - | chr7.2:34961140-34961159 | MS.gene77361:CDS | 45.0% | |
| GAAAGTGAGGGAGATCACAA+GGG | - | chr7.2:34961204-34961223 | MS.gene77361:CDS | 45.0% | |
| GAAGGAGGGTCAAAGAAGAA+AGG | - | chr7.2:34961138-34961157 | MS.gene77361:CDS | 45.0% | |
| GAAGGATTAGAGAGGAAGCT+AGG | - | chr7.2:34961484-34961503 | MS.gene77361:CDS | 45.0% | |
| GCAAATGGAAGCTCAATGGA+TGG | - | chr7.2:34961338-34961357 | MS.gene77361:CDS | 45.0% | |
| GGAGAATGAGAGGAGATTGA+AGG | - | chr7.2:34961377-34961396 | MS.gene77361:CDS | 45.0% | |
| GGAGATTGAAGGAGATGGAA+TGG | - | chr7.2:34961388-34961407 | MS.gene77361:CDS | 45.0% | |
| TGAGAGGAGATTGAAGGAGA+TGG | - | chr7.2:34961383-34961402 | MS.gene77361:CDS | 45.0% | |
| !! | GATGGAAGCATTTGAGGCAA+GGG | - | chr7.2:34961356-34961375 | MS.gene77361:CDS | 45.0% |
| !! | TGGAAGCATTGGAGAATGAG+AGG | - | chr7.2:34961421-34961440 | MS.gene77361:CDS | 45.0% |
| TTGAGGCAAGGGAGAATGAG+AGG | - | chr7.2:34961367-34961386 | MS.gene77361:CDS | 50.0% | |
| ! | AGGGGTGCATGTATCTTCTG+AGG | - | chr7.2:34961158-34961177 | MS.gene77361:CDS | 50.0% |
| ! | ATGCTATGGGCTGAAGCAGA+AGG | - | chr7.2:34961120-34961139 | MS.gene77361:CDS | 50.0% |
| ! | TATGGGCTGAAGCAGAAGGA+GGG | - | chr7.2:34961124-34961143 | MS.gene77361:CDS | 50.0% |
| !! | GGATGGAAGCATTTGAGGCA+AGG | - | chr7.2:34961355-34961374 | MS.gene77361:CDS | 50.0% |
| ! | CTATGGGCTGAAGCAGAAGG+AGG | - | chr7.2:34961123-34961142 | MS.gene77361:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 34961042 | 34961569 | 34961042 | ID=MS.gene77361 |
| chr7.2 | mRNA | 34961042 | 34961569 | 34961042 | ID=MS.gene77361.t1;Parent=MS.gene77361 |
| chr7.2 | exon | 34961042 | 34961569 | 34961042 | ID=MS.gene77361.t1.exon1;Parent=MS.gene77361.t1 |
| chr7.2 | CDS | 34961042 | 34961569 | 34961042 | ID=cds.MS.gene77361.t1;Parent=MS.gene77361.t1 |
| Gene Sequence |
| Protein sequence |
