Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49816.t1 | XP_013451547.1 | 95.2 | 63 | 3 | 0 | 1 | 63 | 1 | 63 | 3.50E-10 | 74.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49816.t1 | I3SGW9 | 95.2 | 63 | 3 | 0 | 1 | 63 | 1 | 63 | 2.5e-10 | 74.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049166 | MS.gene49816 | 0.807029 | 6.17E-50 | -1.69E-46 |
| MS.gene049368 | MS.gene49816 | 0.802693 | 4.97E-49 | -1.69E-46 |
| MS.gene049786 | MS.gene49816 | 0.806037 | 9.99E-50 | -1.69E-46 |
| MS.gene050084 | MS.gene49816 | 0.824247 | 8.95E-54 | -1.69E-46 |
| MS.gene050085 | MS.gene49816 | 0.850149 | 2.05E-60 | -1.69E-46 |
| MS.gene050114 | MS.gene49816 | 0.80695 | 6.41E-50 | -1.69E-46 |
| MS.gene050317 | MS.gene49816 | 0.840951 | 6.38E-58 | -1.69E-46 |
| MS.gene050320 | MS.gene49816 | 0.804556 | 2.04E-49 | -1.69E-46 |
| MS.gene050359 | MS.gene49816 | 0.804526 | 2.07E-49 | -1.69E-46 |
| MS.gene050386 | MS.gene49816 | 0.801355 | 9.36E-49 | -1.69E-46 |
| MS.gene050752 | MS.gene49816 | 0.838353 | 3.03E-57 | -1.69E-46 |
| MS.gene050838 | MS.gene49816 | 0.812973 | 3.24E-51 | -1.69E-46 |
| MS.gene050941 | MS.gene49816 | 0.800464 | 1.42E-48 | -1.69E-46 |
| MS.gene051003 | MS.gene49816 | 0.850428 | 1.71E-60 | -1.69E-46 |
| MS.gene051184 | MS.gene49816 | 0.839835 | 1.25E-57 | -1.69E-46 |
| MS.gene051233 | MS.gene49816 | 0.800096 | 1.69E-48 | -1.69E-46 |
| MS.gene051245 | MS.gene49816 | 0.808107 | 3.64E-50 | -1.69E-46 |
| MS.gene051343 | MS.gene49816 | 0.829982 | 3.79E-55 | -1.69E-46 |
| MS.gene051676 | MS.gene49816 | 0.816171 | 6.35E-52 | -1.69E-46 |
| MS.gene051748 | MS.gene49816 | 0.819693 | 1.02E-52 | -1.69E-46 |
| MS.gene051756 | MS.gene49816 | 0.833875 | 4.15E-56 | -1.69E-46 |
| MS.gene051757 | MS.gene49816 | 0.813466 | 2.52E-51 | -1.69E-46 |
| MS.gene051831 | MS.gene49816 | 0.80207 | 6.68E-49 | -1.69E-46 |
| MS.gene051856 | MS.gene49816 | 0.821928 | 3.11E-53 | -1.69E-46 |
| MS.gene051903 | MS.gene49816 | 0.823498 | 1.34E-53 | -1.69E-46 |
| MS.gene051975 | MS.gene49816 | 0.820812 | 5.63E-53 | -1.69E-46 |
| MS.gene052072 | MS.gene49816 | 0.817347 | 3.46E-52 | -1.69E-46 |
| MS.gene052242 | MS.gene49816 | -0.801822 | 7.51E-49 | -1.69E-46 |
| MS.gene052296 | MS.gene49816 | 0.823991 | 1.03E-53 | -1.69E-46 |
| MS.gene052434 | MS.gene49816 | 0.841225 | 5.41E-58 | -1.69E-46 |
| MS.gene053058 | MS.gene49816 | 0.806825 | 6.81E-50 | -1.69E-46 |
| MS.gene053109 | MS.gene49816 | 0.806122 | 9.59E-50 | -1.69E-46 |
| MS.gene053167 | MS.gene49816 | 0.800853 | 1.19E-48 | -1.69E-46 |
| MS.gene053295 | MS.gene49816 | 0.826983 | 2.01E-54 | -1.69E-46 |
| MS.gene053381 | MS.gene49816 | 0.803637 | 3.17E-49 | -1.69E-46 |
| MS.gene05344 | MS.gene49816 | -0.80721 | 5.65E-50 | -1.69E-46 |
| MS.gene053564 | MS.gene49816 | 0.804594 | 2.00E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene49816 | 0.820827 | 5.58E-53 | -1.69E-46 |
| MS.gene053952 | MS.gene49816 | 0.800562 | 1.36E-48 | -1.69E-46 |
| MS.gene054403 | MS.gene49816 | 0.834399 | 3.07E-56 | -1.69E-46 |
| MS.gene05469 | MS.gene49816 | 0.840176 | 1.02E-57 | -1.69E-46 |
| MS.gene055311 | MS.gene49816 | 0.819083 | 1.40E-52 | -1.69E-46 |
| MS.gene055441 | MS.gene49816 | 0.810021 | 1.42E-50 | -1.69E-46 |
| MS.gene055603 | MS.gene49816 | 0.809099 | 2.24E-50 | -1.69E-46 |
| MS.gene056181 | MS.gene49816 | 0.844902 | 5.67E-59 | -1.69E-46 |
| MS.gene056787 | MS.gene49816 | 0.849989 | 2.27E-60 | -1.69E-46 |
| MS.gene056849 | MS.gene49816 | 0.810961 | 8.89E-51 | -1.69E-46 |
| MS.gene056986 | MS.gene49816 | 0.831631 | 1.50E-55 | -1.69E-46 |
| MS.gene057055 | MS.gene49816 | 0.802371 | 5.79E-49 | -1.69E-46 |
| MS.gene057094 | MS.gene49816 | 0.820869 | 5.46E-53 | -1.69E-46 |
| MS.gene057140 | MS.gene49816 | 0.808176 | 3.52E-50 | -1.69E-46 |
| MS.gene057234 | MS.gene49816 | 0.848721 | 5.12E-60 | -1.69E-46 |
| MS.gene05781 | MS.gene49816 | 0.819574 | 1.08E-52 | -1.69E-46 |
| MS.gene057890 | MS.gene49816 | -0.809098 | 2.24E-50 | -1.69E-46 |
| MS.gene057999 | MS.gene49816 | 0.837672 | 4.53E-57 | -1.69E-46 |
| MS.gene058054 | MS.gene49816 | 0.83884 | 2.26E-57 | -1.69E-46 |
| MS.gene058055 | MS.gene49816 | 0.838203 | 3.31E-57 | -1.69E-46 |
| MS.gene058238 | MS.gene49816 | 0.818812 | 1.61E-52 | -1.69E-46 |
| MS.gene058292 | MS.gene49816 | 0.812002 | 5.28E-51 | -1.69E-46 |
| MS.gene058639 | MS.gene49816 | 0.807138 | 5.85E-50 | -1.69E-46 |
| MS.gene058654 | MS.gene49816 | 0.808554 | 2.93E-50 | -1.69E-46 |
| MS.gene058906 | MS.gene49816 | 0.800628 | 1.32E-48 | -1.69E-46 |
| MS.gene059050 | MS.gene49816 | 0.841726 | 3.99E-58 | -1.69E-46 |
| MS.gene059146 | MS.gene49816 | 0.836972 | 6.84E-57 | -1.69E-46 |
| MS.gene059171 | MS.gene49816 | 0.801717 | 7.89E-49 | -1.69E-46 |
| MS.gene059360 | MS.gene49816 | 0.828115 | 1.08E-54 | -1.69E-46 |
| MS.gene06010 | MS.gene49816 | 0.847714 | 9.71E-60 | -1.69E-46 |
| MS.gene06011 | MS.gene49816 | 0.84437 | 7.88E-59 | -1.69E-46 |
| MS.gene060465 | MS.gene49816 | 0.800339 | 1.51E-48 | -1.69E-46 |
| MS.gene060702 | MS.gene49816 | 0.830608 | 2.67E-55 | -1.69E-46 |
| MS.gene060763 | MS.gene49816 | 0.832491 | 9.17E-56 | -1.69E-46 |
| MS.gene060869 | MS.gene49816 | 0.817552 | 3.11E-52 | -1.69E-46 |
| MS.gene060870 | MS.gene49816 | 0.829075 | 6.30E-55 | -1.69E-46 |
| MS.gene060935 | MS.gene49816 | 0.800001 | 1.77E-48 | -1.69E-46 |
| MS.gene060937 | MS.gene49816 | 0.807565 | 4.75E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49816.t1 | MTR_6g027810 | 97.000 | 100 | 3 | 0 | 1 | 100 | 1 | 100 | 6.64e-55 | 166 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49816.t1 | AT1G54410 | 53.247 | 77 | 27 | 2 | 1 | 76 | 1 | 69 | 3.51e-11 | 55.1 |
Find 19 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAAGGATAAGAAGAAGA+AGG | 0.460441 | 6.3:+19770308 | MS.gene49816:CDS |
| GCAGGAATCATCAACAAGAT+TGG | 0.467463 | 6.3:+19770078 | MS.gene49816:CDS |
| GACAAGATCCATGGTGGTGA+AGG | 0.514151 | 6.3:+19770246 | MS.gene49816:CDS |
| AGGTATAGCTGAGAAGATCA+AGG | 0.553688 | 6.3:+19770224 | MS.gene49816:CDS |
| CACAAAGGTGAACACAAAGA+AGG | 0.571597 | 6.3:+19770204 | MS.gene49816:CDS |
| AGTCACAAACAAGTCATGGC+AGG | 0.585630 | 6.3:+19770060 | None:intergenic |
| ATTGGAGAGGCTCTTCATGT+AGG | 0.594022 | 6.3:+19770096 | MS.gene49816:CDS |
| GAGGCTCTTCATGTAGGAGG+AGG | 0.594348 | 6.3:+19770102 | MS.gene49816:CDS |
| ATCCATGGTGGTGAAGGTGA+AGG | 0.602593 | 6.3:+19770252 | MS.gene49816:CDS |
| GGAGAGGCTCTTCATGTAGG+AGG | 0.607476 | 6.3:+19770099 | MS.gene49816:CDS |
| AAGATCAAGGACAAGATCCA+TGG | 0.615180 | 6.3:+19770237 | MS.gene49816:CDS |
| GACCTTCACCTTCACCACCA+TGG | 0.643247 | 6.3:-19770254 | None:intergenic |
| GATAAGAAGAAGAAGGAACA+TGG | 0.650367 | 6.3:+19770315 | MS.gene49816:CDS |
| AAGAAAGAAGATGAACACAA+AGG | 0.654433 | 6.3:+19770129 | MS.gene49816:CDS |
| AATCATCAACAAGATTGGAG+AGG | 0.688291 | 6.3:+19770083 | MS.gene49816:CDS |
| AGTCATGATGACAAGCACAA+AGG | 0.694046 | 6.3:+19770159 | MS.gene49816:CDS |
| AGTCATGATGATAAGCACAA+AGG | 0.702046 | 6.3:+19770189 | MS.gene49816:CDS |
| ATCAAGGACAAGATCCATGG+TGG | 0.715898 | 6.3:+19770240 | MS.gene49816:CDS |
| GGTGAAGGTCATGAACACAA+AGG | 0.734951 | 6.3:+19770267 | MS.gene49816:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GAAGAAAGATAAGAAAAAGA+AGG | + | chr6.3:19770293-19770312 | MS.gene49816:CDS | 25.0% |
| AAAGAAGGATAAGAAGAAGA+AGG | + | chr6.3:19770308-19770327 | MS.gene49816:CDS | 30.0% | |
| AAGAAAGAAGATGAACACAA+AGG | + | chr6.3:19770129-19770148 | MS.gene49816:CDS | 30.0% | |
| AATCATCAACAAGATTGGAG+AGG | + | chr6.3:19770083-19770102 | MS.gene49816:CDS | 35.0% | |
| AGTCATGATGATAAGCACAA+AGG | + | chr6.3:19770189-19770208 | MS.gene49816:CDS | 35.0% | |
| GATAAGAAGAAGAAGGAACA+TGG | + | chr6.3:19770315-19770334 | MS.gene49816:CDS | 35.0% | |
| AAGATCAAGGACAAGATCCA+TGG | + | chr6.3:19770237-19770256 | MS.gene49816:CDS | 40.0% | |
| AGGTATAGCTGAGAAGATCA+AGG | + | chr6.3:19770224-19770243 | MS.gene49816:CDS | 40.0% | |
| AGTCATGATGACAAGCACAA+AGG | + | chr6.3:19770159-19770178 | MS.gene49816:CDS | 40.0% | |
| CACAAAGGTGAACACAAAGA+AGG | + | chr6.3:19770204-19770223 | MS.gene49816:CDS | 40.0% | |
| GCAGGAATCATCAACAAGAT+TGG | + | chr6.3:19770078-19770097 | MS.gene49816:CDS | 40.0% | |
| ATCAAGGACAAGATCCATGG+TGG | + | chr6.3:19770240-19770259 | MS.gene49816:CDS | 45.0% | |
| ATTGGAGAGGCTCTTCATGT+AGG | + | chr6.3:19770096-19770115 | MS.gene49816:CDS | 45.0% | |
| GGTGAAGGTCATGAACACAA+AGG | + | chr6.3:19770267-19770286 | MS.gene49816:CDS | 45.0% | |
| ! | ATCCATGGTGGTGAAGGTGA+AGG | + | chr6.3:19770252-19770271 | MS.gene49816:CDS | 50.0% |
| ! | GACAAGATCCATGGTGGTGA+AGG | + | chr6.3:19770246-19770265 | MS.gene49816:CDS | 50.0% |
| GACCTTCACCTTCACCACCA+TGG | - | chr6.3:19770257-19770276 | None:intergenic | 55.0% | |
| GAGGCTCTTCATGTAGGAGG+AGG | + | chr6.3:19770102-19770121 | MS.gene49816:CDS | 55.0% | |
| GGAGAGGCTCTTCATGTAGG+AGG | + | chr6.3:19770099-19770118 | MS.gene49816:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 19770075 | 19770377 | 19770075 | ID=MS.gene49816 |
| chr6.3 | mRNA | 19770075 | 19770377 | 19770075 | ID=MS.gene49816.t1;Parent=MS.gene49816 |
| chr6.3 | exon | 19770075 | 19770377 | 19770075 | ID=MS.gene49816.t1.exon1;Parent=MS.gene49816.t1 |
| chr6.3 | CDS | 19770075 | 19770377 | 19770075 | ID=cds.MS.gene49816.t1;Parent=MS.gene49816.t1 |
| Gene Sequence |
| Protein sequence |
