Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71525.t1 | XP_003601570.1 | 99 | 315 | 3 | 0 | 1 | 315 | 1 | 315 | 2.70E-173 | 617.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71525.t1 | P26690 | 68.9 | 315 | 97 | 1 | 1 | 315 | 1 | 314 | 3.5e-129 | 462.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71525.t1 | G7J9I2 | 99.0 | 315 | 3 | 0 | 1 | 315 | 1 | 315 | 1.9e-173 | 617.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050380 | MS.gene71525 | 0.860093 | 2.63E-63 | -1.69E-46 |
| MS.gene050381 | MS.gene71525 | 0.877163 | 7.83E-69 | -1.69E-46 |
| MS.gene050502 | MS.gene71525 | -0.802494 | 5.46E-49 | -1.69E-46 |
| MS.gene050868 | MS.gene71525 | -0.802005 | 6.89E-49 | -1.69E-46 |
| MS.gene050871 | MS.gene71525 | -0.807346 | 5.29E-50 | -1.69E-46 |
| MS.gene050883 | MS.gene71525 | 0.820228 | 7.66E-53 | -1.69E-46 |
| MS.gene051127 | MS.gene71525 | 0.828234 | 1.01E-54 | -1.69E-46 |
| MS.gene051946 | MS.gene71525 | 0.803313 | 3.70E-49 | -1.69E-46 |
| MS.gene053319 | MS.gene71525 | 0.834474 | 2.93E-56 | -1.69E-46 |
| MS.gene053406 | MS.gene71525 | 0.810733 | 9.96E-51 | -1.69E-46 |
| MS.gene053436 | MS.gene71525 | -0.800675 | 1.29E-48 | -1.69E-46 |
| MS.gene053574 | MS.gene71525 | -0.809753 | 1.62E-50 | -1.69E-46 |
| MS.gene054003 | MS.gene71525 | -0.804414 | 2.19E-49 | -1.69E-46 |
| MS.gene054595 | MS.gene71525 | -0.812794 | 3.54E-51 | -1.69E-46 |
| MS.gene055124 | MS.gene71525 | 0.863289 | 2.77E-64 | -1.69E-46 |
| MS.gene055139 | MS.gene71525 | 0.804948 | 1.69E-49 | -1.69E-46 |
| MS.gene055154 | MS.gene71525 | 0.823568 | 1.29E-53 | -1.69E-46 |
| MS.gene055401 | MS.gene71525 | 0.827226 | 1.76E-54 | -1.69E-46 |
| MS.gene055959 | MS.gene71525 | 0.809545 | 1.79E-50 | -1.69E-46 |
| MS.gene056166 | MS.gene71525 | -0.82841 | 9.13E-55 | -1.69E-46 |
| MS.gene056310 | MS.gene71525 | 0.870535 | 1.36E-66 | -1.69E-46 |
| MS.gene056311 | MS.gene71525 | 0.82607 | 3.32E-54 | -1.69E-46 |
| MS.gene056313 | MS.gene71525 | 0.902698 | 7.43E-79 | -1.69E-46 |
| MS.gene056408 | MS.gene71525 | 0.806728 | 7.14E-50 | -1.69E-46 |
| MS.gene056422 | MS.gene71525 | -0.8394 | 1.62E-57 | -1.69E-46 |
| MS.gene057086 | MS.gene71525 | -0.804927 | 1.71E-49 | -1.69E-46 |
| MS.gene057506 | MS.gene71525 | 0.804028 | 2.63E-49 | -1.69E-46 |
| MS.gene058099 | MS.gene71525 | 0.812554 | 4.00E-51 | -1.69E-46 |
| MS.gene058108 | MS.gene71525 | 0.844059 | 9.56E-59 | -1.69E-46 |
| MS.gene058109 | MS.gene71525 | 0.839081 | 1.96E-57 | -1.69E-46 |
| MS.gene058170 | MS.gene71525 | 0.829554 | 4.82E-55 | -1.69E-46 |
| MS.gene058171 | MS.gene71525 | 0.85219 | 5.43E-61 | -1.69E-46 |
| MS.gene058173 | MS.gene71525 | 0.846759 | 1.78E-59 | -1.69E-46 |
| MS.gene058174 | MS.gene71525 | 0.833548 | 5.01E-56 | -1.69E-46 |
| MS.gene058175 | MS.gene71525 | 0.851702 | 7.47E-61 | -1.69E-46 |
| MS.gene058176 | MS.gene71525 | 0.835029 | 2.13E-56 | -1.69E-46 |
| MS.gene058179 | MS.gene71525 | 0.861254 | 1.17E-63 | -1.69E-46 |
| MS.gene058180 | MS.gene71525 | 0.867262 | 1.56E-65 | -1.69E-46 |
| MS.gene05837 | MS.gene71525 | -0.807572 | 4.73E-50 | -1.69E-46 |
| MS.gene058840 | MS.gene71525 | 0.83707 | 6.46E-57 | -1.69E-46 |
| MS.gene059037 | MS.gene71525 | 0.857666 | 1.40E-62 | -1.69E-46 |
| MS.gene059038 | MS.gene71525 | 0.80416 | 2.47E-49 | -1.69E-46 |
| MS.gene059044 | MS.gene71525 | 0.827784 | 1.29E-54 | -1.69E-46 |
| MS.gene059045 | MS.gene71525 | 0.825953 | 3.54E-54 | -1.69E-46 |
| MS.gene059367 | MS.gene71525 | -0.824275 | 8.82E-54 | -1.69E-46 |
| MS.gene059368 | MS.gene71525 | -0.818829 | 1.60E-52 | -1.69E-46 |
| MS.gene059517 | MS.gene71525 | -0.822772 | 1.98E-53 | -1.69E-46 |
| MS.gene059690 | MS.gene71525 | 0.801721 | 7.88E-49 | -1.69E-46 |
| MS.gene059691 | MS.gene71525 | 0.813235 | 2.84E-51 | -1.69E-46 |
| MS.gene059694 | MS.gene71525 | 0.817824 | 2.70E-52 | -1.69E-46 |
| MS.gene059697 | MS.gene71525 | 0.836149 | 1.11E-56 | -1.69E-46 |
| MS.gene059944 | MS.gene71525 | 0.833764 | 4.42E-56 | -1.69E-46 |
| MS.gene060482 | MS.gene71525 | -0.842976 | 1.86E-58 | -1.69E-46 |
| MS.gene060808 | MS.gene71525 | 0.831315 | 1.79E-55 | -1.69E-46 |
| MS.gene060837 | MS.gene71525 | -0.810377 | 1.19E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene040151 | MS.gene71525 | PPI |
| MS.gene71525 | MS.gene72560 | PPI |
| MS.gene71525 | MS.gene024362 | PPI |
| MS.gene71525 | MS.gene91446 | PPI |
| MS.gene71525 | MS.gene008794 | PPI |
| MS.gene71525 | MS.gene95889 | PPI |
| MS.gene71525 | MS.gene54933 | PPI |
| MS.gene71525 | MS.gene98697 | PPI |
| MS.gene71525 | MS.gene93059 | PPI |
| MS.gene71525 | MS.gene93783 | PPI |
| MS.gene71525 | MS.gene93058 | PPI |
| MS.gene71525 | MS.gene55382 | PPI |
| MS.gene71525 | MS.gene99693 | PPI |
| MS.gene71525 | MS.gene003638 | PPI |
| MS.gene71525 | MS.gene42146 | PPI |
| MS.gene71525 | MS.gene056488 | PPI |
| MS.gene71525 | MS.gene62620 | PPI |
| MS.gene71525 | MS.gene97980 | PPI |
| MS.gene71525 | MS.gene97981 | PPI |
| MS.gene71525 | MS.gene056487 | PPI |
| MS.gene71525 | MS.gene062932 | PPI |
| MS.gene71525 | MS.gene50700 | PPI |
| MS.gene71525 | MS.gene008793 | PPI |
| MS.gene71525 | MS.gene42873 | PPI |
| MS.gene71525 | MS.gene87111 | PPI |
| MS.gene71525 | MS.gene23359 | PPI |
| MS.gene71525 | MS.gene040151 | PPI |
| MS.gene71525 | MS.gene53169 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71525.t1 | MTR_3g083130 | 99.048 | 315 | 3 | 0 | 1 | 315 | 1 | 315 | 0.0 | 637 |
| MS.gene71525.t1 | MTR_5g097900 | 68.506 | 308 | 96 | 1 | 7 | 314 | 6 | 312 | 4.86e-154 | 434 |
| MS.gene71525.t1 | MTR_5g097910 | 68.182 | 308 | 97 | 1 | 7 | 314 | 6 | 312 | 1.12e-153 | 433 |
| MS.gene71525.t1 | MTR_5g097940 | 68.182 | 308 | 97 | 1 | 7 | 314 | 6 | 312 | 1.12e-153 | 433 |
| MS.gene71525.t1 | MTR_0374s0050 | 62.857 | 315 | 114 | 3 | 3 | 315 | 2 | 315 | 5.74e-143 | 406 |
| MS.gene71525.t1 | MTR_4g072320 | 50.633 | 316 | 138 | 5 | 7 | 313 | 6 | 312 | 2.05e-106 | 313 |
| MS.gene71525.t1 | MTR_6g073110 | 51.325 | 302 | 136 | 4 | 6 | 302 | 5 | 300 | 1.09e-103 | 306 |
| MS.gene71525.t1 | MTR_4g072350 | 49.686 | 318 | 144 | 6 | 7 | 313 | 6 | 318 | 1.86e-103 | 306 |
| MS.gene71525.t1 | MTR_4g072360 | 48.910 | 321 | 148 | 6 | 4 | 313 | 3 | 318 | 1.46e-102 | 303 |
| MS.gene71525.t1 | MTR_4g036845 | 40.328 | 305 | 168 | 7 | 21 | 315 | 14 | 314 | 6.42e-75 | 233 |
| MS.gene71525.t1 | MTR_8g070095 | 40.702 | 285 | 165 | 3 | 20 | 302 | 15 | 297 | 7.36e-71 | 223 |
| MS.gene71525.t1 | MTR_4g021350 | 45.230 | 283 | 149 | 5 | 20 | 302 | 15 | 291 | 1.91e-69 | 219 |
| MS.gene71525.t1 | MTR_8g070115 | 40.656 | 305 | 167 | 5 | 20 | 315 | 15 | 314 | 8.52e-68 | 214 |
| MS.gene71525.t1 | MTR_7g063580 | 41.311 | 305 | 164 | 5 | 20 | 315 | 55 | 353 | 6.65e-67 | 213 |
| MS.gene71525.t1 | MTR_4g021350 | 44.765 | 277 | 147 | 5 | 20 | 296 | 15 | 285 | 1.28e-66 | 211 |
| MS.gene71525.t1 | MTR_4g021410 | 41.118 | 304 | 156 | 5 | 20 | 315 | 16 | 304 | 1.48e-64 | 206 |
| MS.gene71525.t1 | MTR_7g070500 | 37.134 | 307 | 172 | 7 | 21 | 315 | 23 | 320 | 1.31e-62 | 201 |
| MS.gene71525.t1 | MTR_4g021450 | 40.461 | 304 | 158 | 5 | 20 | 315 | 16 | 304 | 1.26e-61 | 198 |
| MS.gene71525.t1 | MTR_4g021410 | 41.455 | 275 | 140 | 4 | 49 | 315 | 6 | 267 | 1.76e-60 | 194 |
| MS.gene71525.t1 | MTR_7g070500 | 38.545 | 275 | 160 | 5 | 21 | 294 | 23 | 289 | 3.89e-60 | 194 |
| MS.gene71525.t1 | MTR_4g072060 | 37.171 | 304 | 175 | 6 | 20 | 312 | 10 | 308 | 1.64e-52 | 175 |
| MS.gene71525.t1 | MTR_4g021410 | 41.250 | 240 | 121 | 3 | 84 | 315 | 1 | 228 | 2.83e-49 | 164 |
| MS.gene71525.t1 | MTR_4g021450 | 40.833 | 240 | 122 | 3 | 84 | 315 | 1 | 228 | 2.74e-47 | 159 |
| MS.gene71525.t1 | MTR_4g021450 | 39.130 | 184 | 101 | 2 | 140 | 315 | 5 | 185 | 1.61e-39 | 137 |
| MS.gene71525.t1 | MTR_6g072650 | 45.333 | 150 | 46 | 5 | 175 | 313 | 10 | 134 | 7.96e-29 | 108 |
| MS.gene71525.t1 | MTR_6g072710 | 47.788 | 113 | 54 | 2 | 193 | 302 | 1 | 111 | 1.17e-26 | 101 |
| MS.gene71525.t1 | MTR_3g449790 | 25.000 | 308 | 162 | 10 | 39 | 308 | 77 | 353 | 2.15e-12 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71525.t1 | AT1G59960 | 51.692 | 325 | 138 | 7 | 6 | 315 | 5 | 325 | 5.18e-114 | 333 |
| MS.gene71525.t1 | AT1G59950 | 52.681 | 317 | 138 | 5 | 10 | 315 | 4 | 319 | 7.11e-109 | 320 |
| MS.gene71525.t1 | AT5G01670 | 40.645 | 310 | 159 | 9 | 20 | 315 | 23 | 321 | 3.74e-73 | 228 |
| MS.gene71525.t1 | AT2G37790 | 42.105 | 304 | 163 | 6 | 20 | 315 | 15 | 313 | 4.85e-72 | 225 |
| MS.gene71525.t1 | AT2G37770 | 41.503 | 306 | 163 | 6 | 20 | 315 | 15 | 314 | 1.62e-71 | 224 |
| MS.gene71525.t1 | AT5G62420 | 39.869 | 306 | 169 | 7 | 21 | 315 | 14 | 315 | 7.94e-71 | 222 |
| MS.gene71525.t1 | AT5G01670 | 37.389 | 337 | 159 | 10 | 20 | 315 | 23 | 348 | 3.24e-67 | 214 |
| MS.gene71525.t1 | AT2G37760 | 39.016 | 305 | 168 | 5 | 20 | 315 | 15 | 310 | 2.21e-66 | 211 |
| MS.gene71525.t1 | AT2G37760 | 39.016 | 305 | 168 | 5 | 20 | 315 | 15 | 310 | 2.21e-66 | 211 |
| MS.gene71525.t1 | AT2G37760 | 41.071 | 280 | 155 | 4 | 20 | 298 | 15 | 285 | 4.05e-66 | 209 |
| MS.gene71525.t1 | AT2G37760 | 40.426 | 282 | 158 | 4 | 20 | 300 | 15 | 287 | 6.97e-66 | 209 |
| MS.gene71525.t1 | AT2G37760 | 40.426 | 282 | 158 | 4 | 20 | 300 | 15 | 287 | 6.97e-66 | 209 |
| MS.gene71525.t1 | AT3G53880 | 37.908 | 306 | 174 | 6 | 20 | 315 | 15 | 314 | 2.21e-62 | 201 |
| MS.gene71525.t1 | AT3G53880 | 38.129 | 278 | 166 | 4 | 20 | 296 | 15 | 287 | 2.36e-58 | 190 |
| MS.gene71525.t1 | AT2G21250 | 34.539 | 304 | 183 | 6 | 20 | 312 | 10 | 308 | 1.31e-57 | 188 |
| MS.gene71525.t1 | AT2G21260 | 35.417 | 288 | 170 | 6 | 20 | 296 | 10 | 292 | 7.85e-57 | 186 |
| MS.gene71525.t1 | AT2G37760 | 38.168 | 262 | 150 | 4 | 63 | 315 | 6 | 264 | 4.37e-54 | 177 |
| MS.gene71525.t1 | AT2G37760 | 38.168 | 262 | 150 | 4 | 63 | 315 | 6 | 264 | 4.37e-54 | 177 |
| MS.gene71525.t1 | AT2G37770 | 45.833 | 192 | 99 | 3 | 20 | 211 | 15 | 201 | 3.24e-48 | 163 |
| MS.gene71525.t1 | AT2G21250 | 36.681 | 229 | 129 | 6 | 20 | 237 | 10 | 233 | 4.54e-43 | 148 |
| MS.gene71525.t1 | AT2G21260 | 31.797 | 217 | 135 | 4 | 89 | 294 | 8 | 222 | 4.94e-35 | 127 |
| MS.gene71525.t1 | AT1G06690 | 25.763 | 295 | 166 | 7 | 39 | 297 | 88 | 365 | 2.13e-17 | 82.4 |
| MS.gene71525.t1 | AT5G53580 | 25.267 | 281 | 154 | 12 | 45 | 287 | 80 | 342 | 5.96e-12 | 66.2 |
Find 82 sgRNAs with CRISPR-Local
Find 101 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTTGTTACTTCTAAATTA+TGG | 0.129563 | 3.4:-78284322 | MS.gene71525:CDS |
| AGCCTCTTCTTGATGATCTA+TGG | 0.232119 | 3.4:-78283303 | MS.gene71525:CDS |
| CTTTGATGAGTCTCTCTTGA+TGG | 0.239320 | 3.4:+78283335 | None:intergenic |
| AATCAAAGATTTGCAAGTTT+TGG | 0.245163 | 3.4:+78283398 | None:intergenic |
| TCACCTGGTTTAGTAGTAAT+TGG | 0.253992 | 3.4:+78284106 | None:intergenic |
| CTTTGTTACTTCTAAATTAT+GGG | 0.275213 | 3.4:-78284321 | MS.gene71525:CDS |
| ATGTCTATAACCTTGTTTGA+TGG | 0.330198 | 3.4:+78284439 | None:intergenic |
| ACAATCTCTGATACTTCAAT+AGG | 0.347637 | 3.4:+78284073 | None:intergenic |
| AAAACTTGCAAATCTTTGAT+TGG | 0.362637 | 3.4:-78283396 | MS.gene71525:CDS |
| AACCTTGTTTGATGGCTTCA+AGG | 0.381003 | 3.4:+78284447 | None:intergenic |
| CCCTGCTAGCACCTTTCCTA+AGG | 0.387480 | 3.4:+78283701 | None:intergenic |
| CAATGATATACTCAAAGAAT+TGG | 0.390203 | 3.4:-78283659 | MS.gene71525:CDS |
| GTAACCTTACTGGTTGCTTC+CGG | 0.403522 | 3.4:+78284485 | None:intergenic |
| TTTCCGTTGTCCGGTGGAGG+AGG | 0.424568 | 3.4:+78284535 | None:intergenic |
| TTGGCCAATGGATCAAAATA+AGG | 0.425035 | 3.4:+78284125 | None:intergenic |
| CTTAGAGATTTCTGCAGAGC+AGG | 0.429110 | 3.4:+78284269 | None:intergenic |
| CTGCTCTGCAGAAATCTCTA+AGG | 0.431594 | 3.4:-78284268 | MS.gene71525:intron |
| GTTGCTAGCTCCAATGGCTT+TGG | 0.432705 | 3.4:+78283991 | None:intergenic |
| CCCTTAGGAAAGGTGCTAGC+AGG | 0.441076 | 3.4:-78283702 | MS.gene71525:CDS |
| GAGGAGGGGAGGACTACTTC+AGG | 0.445285 | 3.4:+78284551 | None:intergenic |
| CAATCTCTGATACTTCAATA+GGG | 0.455333 | 3.4:+78284074 | None:intergenic |
| GTTTCTCTTGTTGCCATCCA+AGG | 0.461119 | 3.4:+78283773 | None:intergenic |
| TTCCGTTGTCCGGTGGAGGA+GGG | 0.463761 | 3.4:+78284536 | None:intergenic |
| GACTCTTCAACTAGAATACT+TGG | 0.468397 | 3.4:-78284149 | MS.gene71525:CDS |
| AAAATGCCGGTGATGGGCCT+CGG | 0.468907 | 3.4:-78284515 | MS.gene71525:CDS |
| TTTGATGCAGCTGCTGCATA+TGG | 0.474976 | 3.4:-78284416 | MS.gene71525:CDS |
| TCCGTTGTCCGGTGGAGGAG+GGG | 0.476698 | 3.4:+78284537 | None:intergenic |
| GCCTCTTCTTGATGATCTAT+GGG | 0.483427 | 3.4:-78283302 | MS.gene71525:CDS |
| CCTGCTAGCACCTTTCCTAA+GGG | 0.491638 | 3.4:+78283702 | None:intergenic |
| TGAGAAGTTGCTAGCTCCAA+TGG | 0.493072 | 3.4:+78283985 | None:intergenic |
| ACAACGGAAAATGCCGGTGA+TGG | 0.497720 | 3.4:-78284522 | MS.gene71525:CDS |
| CTTGGTCTCACCAAAGCCAT+TGG | 0.503945 | 3.4:-78284001 | MS.gene71525:CDS |
| TCAATTACTTGATTCACTGC+AGG | 0.507920 | 3.4:+78283920 | None:intergenic |
| TTTGGCAGATTTGTCTTCGA+TGG | 0.511955 | 3.4:-78283477 | MS.gene71525:intron |
| GAAAGGTGTGTGGACATCAT+TGG | 0.513831 | 3.4:-78284038 | MS.gene71525:CDS |
| ATTGCAGAAGCACTTAAACT+TGG | 0.516828 | 3.4:-78284368 | MS.gene71525:CDS |
| CAACGGAAAATGCCGGTGAT+GGG | 0.522929 | 3.4:-78284521 | MS.gene71525:CDS |
| TAACTGCCTTCTCACCCCTT+AGG | 0.527768 | 3.4:-78283717 | MS.gene71525:CDS |
| CCTTAGGAAAGGTGCTAGCA+GGG | 0.529822 | 3.4:-78283701 | MS.gene71525:CDS |
| ATAGGGTATTTAACTTCACC+TGG | 0.535676 | 3.4:+78284091 | None:intergenic |
| TTGCTTCCGGTGCGGTGCCG+AGG | 0.538965 | 3.4:+78284498 | None:intergenic |
| GTAGTCCTCCCCTCCTCCAC+CGG | 0.540364 | 3.4:-78284545 | MS.gene71525:CDS |
| TTGATTCACTGCAGGAGGGA+TGG | 0.540989 | 3.4:+78283928 | None:intergenic |
| ACCTTTCCTAAGGGGTGAGA+AGG | 0.541355 | 3.4:+78283711 | None:intergenic |
| GTTGTCCGGTGGAGGAGGGG+AGG | 0.547684 | 3.4:+78284540 | None:intergenic |
| AGGTGGAAGTCAACCTTGGA+TGG | 0.547815 | 3.4:-78283786 | MS.gene71525:CDS |
| GATGGGCCTCGGCACCGCAC+CGG | 0.552144 | 3.4:-78284504 | MS.gene71525:CDS |
| TATTGAAGTATCAGAGATTG+TGG | 0.554195 | 3.4:-78284071 | MS.gene71525:CDS |
| AGGACTACTTCAGGGACTGT+AGG | 0.556376 | 3.4:+78284560 | None:intergenic |
| TAGATCATCAAGAAGAGGCT+TGG | 0.556426 | 3.4:+78283306 | None:intergenic |
| TCATCAAGAAGAGGCTTGGT+TGG | 0.556818 | 3.4:+78283310 | None:intergenic |
| AGAGCAGGAACAATGAGCTC+AGG | 0.564156 | 3.4:+78284284 | None:intergenic |
| ATTACTTGATTCACTGCAGG+AGG | 0.566545 | 3.4:+78283923 | None:intergenic |
| TCCCCTCCTCCACCGGACAA+CGG | 0.566967 | 3.4:-78284538 | MS.gene71525:CDS |
| GCGGTGCCGAGGCCCATCAC+CGG | 0.568831 | 3.4:+78284509 | None:intergenic |
| TAATAGGTGGAAGTCAACCT+TGG | 0.576953 | 3.4:-78283790 | MS.gene71525:intron |
| GTTTAGTAGTAATTGGCCAA+TGG | 0.578679 | 3.4:+78284113 | None:intergenic |
| TGCAGGAGGGATGGTTGCAA+AGG | 0.578968 | 3.4:+78283937 | None:intergenic |
| TCCCATAGATCATCAAGAAG+AGG | 0.583002 | 3.4:+78283301 | None:intergenic |
| CGCACCGGAAGCAACCAGTA+AGG | 0.584380 | 3.4:-78284489 | MS.gene71525:CDS |
| ACATGGCAAGACTATAGCTC+AGG | 0.585566 | 3.4:-78283629 | MS.gene71525:intron |
| CTTCGATGGCTATATGAGCA+AGG | 0.590577 | 3.4:-78283463 | MS.gene71525:CDS |
| GTCCTTGAAGCCATCAAACA+AGG | 0.591634 | 3.4:-78284449 | MS.gene71525:CDS |
| CACCGGACAACGGAAAATGC+CGG | 0.597803 | 3.4:-78284528 | MS.gene71525:CDS |
| CAGGGGTGCTAATTTAGTGA+TGG | 0.600153 | 3.4:-78283683 | MS.gene71525:CDS |
| AGGAGGGGAGGACTACTTCA+GGG | 0.602610 | 3.4:+78284552 | None:intergenic |
| TTAAGAGCTATGACAAGGAG+AGG | 0.604664 | 3.4:-78283426 | MS.gene71525:CDS |
| GCCTTCTCACCCCTTAGGAA+AGG | 0.609336 | 3.4:-78283712 | MS.gene71525:CDS |
| AAAGAATTGGCTGATGCACA+TGG | 0.613616 | 3.4:-78283646 | MS.gene71525:CDS |
| CTTTGATTGGTCATTGACTG+AGG | 0.619859 | 3.4:-78283383 | MS.gene71525:CDS |
| CTTACTGGTTGCTTCCGGTG+CGG | 0.628936 | 3.4:+78284490 | None:intergenic |
| CTGCTAGCACCTTTCCTAAG+GGG | 0.635830 | 3.4:+78283703 | None:intergenic |
| TGTGGTTAAGAGCTATGACA+AGG | 0.641038 | 3.4:-78283431 | MS.gene71525:CDS |
| CATCAAGAGAGACTCATCAA+AGG | 0.641861 | 3.4:-78283334 | MS.gene71525:CDS |
| TGGCCAATTACTACTAAACC+AGG | 0.647399 | 3.4:-78284109 | MS.gene71525:CDS |
| ATTGTGGAATTTGATATGAA+AGG | 0.649138 | 3.4:-78284055 | MS.gene71525:CDS |
| GAAACTTAGAGCCTTCTGCA+AGG | 0.650710 | 3.4:-78283755 | MS.gene71525:CDS |
| CTTAGGAAAGGTGCTAGCAG+GGG | 0.654145 | 3.4:-78283700 | MS.gene71525:CDS |
| CTGCAAGGAAAAGTGTATTG+TGG | 0.655539 | 3.4:-78283740 | MS.gene71525:CDS |
| TTACTTGATTCACTGCAGGA+GGG | 0.664815 | 3.4:+78283924 | None:intergenic |
| AATTTGATATGAAAGGTGTG+TGG | 0.691006 | 3.4:-78284048 | MS.gene71525:CDS |
| TGCAGCTGCTGCATATGGTG+TGG | 0.691514 | 3.4:-78284411 | MS.gene71525:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTAATGTCACAATACTT+TGG | - | chr3.4:78284368-78284387 | MS.gene71525:CDS | 20.0% |
| !! | ACATATGAAATCATGTAAAA+TGG | + | chr3.4:78284003-78284022 | None:intergenic | 20.0% |
| !! | TATATAATATGATCTTAGCA+TGG | - | chr3.4:78284266-78284285 | MS.gene71525:intron | 20.0% |
| !!! | AAATTAATTTGGATCCTAAT+AGG | - | chr3.4:78284057-78284076 | MS.gene71525:CDS | 20.0% |
| !!! | CTTTGTTACTTCTAAATTAT+GGG | - | chr3.4:78283542-78283561 | MS.gene71525:intron | 20.0% |
| !!! | TCTTTGTTACTTCTAAATTA+TGG | - | chr3.4:78283541-78283560 | MS.gene71525:intron | 20.0% |
| ! | AAAACTTGCAAATCTTTGAT+TGG | - | chr3.4:78284467-78284486 | MS.gene71525:CDS | 25.0% |
| ! | ATTGTGGAATTTGATATGAA+AGG | - | chr3.4:78283808-78283827 | MS.gene71525:intron | 25.0% |
| ! | CAATGATATACTCAAAGAAT+TGG | - | chr3.4:78284204-78284223 | MS.gene71525:intron | 25.0% |
| !! | AATCAAAGATTTGCAAGTTT+TGG | + | chr3.4:78284468-78284487 | None:intergenic | 25.0% |
| AATCACTCCAAATTAGAAGT+TGG | + | chr3.4:78284293-78284312 | None:intergenic | 30.0% | |
| ACAATCTCTGATACTTCAAT+AGG | + | chr3.4:78283793-78283812 | None:intergenic | 30.0% | |
| CAATCTCTGATACTTCAATA+GGG | + | chr3.4:78283792-78283811 | None:intergenic | 30.0% | |
| TTGGAAGAATGTCAAAAACT+TGG | - | chr3.4:78283844-78283863 | MS.gene71525:intron | 30.0% | |
| ! | AATTTGATATGAAAGGTGTG+TGG | - | chr3.4:78283815-78283834 | MS.gene71525:intron | 30.0% |
| ! | ATCTTTTGTAGTAACCTTAC+TGG | + | chr3.4:78283391-78283410 | None:intergenic | 30.0% |
| ! | ATGTCTATAACCTTGTTTGA+TGG | + | chr3.4:78283427-78283446 | None:intergenic | 30.0% |
| ! | TATTGAAGTATCAGAGATTG+TGG | - | chr3.4:78283792-78283811 | MS.gene71525:intron | 30.0% |
| ! | TTAATTTGGATCCTAATAGG+TGG | - | chr3.4:78284060-78284079 | MS.gene71525:CDS | 30.0% |
| GACTCTTCAACTAGAATACT+TGG | - | chr3.4:78283714-78283733 | MS.gene71525:CDS | 35.0% | |
| GTTTAGTAGTAATTGGCCAA+TGG | + | chr3.4:78283753-78283772 | None:intergenic | 35.0% | |
| TATGGTGTGGAAAAATCTGT+TGG | - | chr3.4:78283465-78283484 | MS.gene71525:CDS | 35.0% | |
| TCAATTACTTGATTCACTGC+AGG | + | chr3.4:78283946-78283965 | None:intergenic | 35.0% | |
| TCACCTGGTTTAGTAGTAAT+TGG | + | chr3.4:78283760-78283779 | None:intergenic | 35.0% | |
| TTGGCCAATGGATCAAAATA+AGG | + | chr3.4:78283741-78283760 | None:intergenic | 35.0% | |
| ! | AATACACTTTTCCTTGCAGA+AGG | + | chr3.4:78284122-78284141 | None:intergenic | 35.0% |
| ! | ATAGGGTATTTAACTTCACC+TGG | + | chr3.4:78283775-78283794 | None:intergenic | 35.0% |
| !! | ATTGCAGAAGCACTTAAACT+TGG | - | chr3.4:78283495-78283514 | MS.gene71525:intron | 35.0% |
| !!! | GTATACATGCTTGTTTTTGC+AGG | - | chr3.4:78283692-78283711 | MS.gene71525:CDS | 35.0% |
| !!! | TGGACCTTATTTTGATCCAT+TGG | - | chr3.4:78283734-78283753 | MS.gene71525:CDS | 35.0% |
| AAAGAATTGGCTGATGCACA+TGG | - | chr3.4:78284217-78284236 | MS.gene71525:intron | 40.0% | |
| ATTACTTGATTCACTGCAGG+AGG | + | chr3.4:78283943-78283962 | None:intergenic | 40.0% | |
| CATCAAGAGAGACTCATCAA+AGG | - | chr3.4:78284529-78284548 | MS.gene71525:CDS | 40.0% | |
| CTTTGATGAGTCTCTCTTGA+TGG | + | chr3.4:78284531-78284550 | None:intergenic | 40.0% | |
| GCATGGACCAACTTCTAATT+TGG | - | chr3.4:78284283-78284302 | MS.gene71525:CDS | 40.0% | |
| TAATAGGTGGAAGTCAACCT+TGG | - | chr3.4:78284073-78284092 | MS.gene71525:CDS | 40.0% | |
| TAGATCATCAAGAAGAGGCT+TGG | + | chr3.4:78284560-78284579 | None:intergenic | 40.0% | |
| TCCCATAGATCATCAAGAAG+AGG | + | chr3.4:78284565-78284584 | None:intergenic | 40.0% | |
| TGGCCAATTACTACTAAACC+AGG | - | chr3.4:78283754-78283773 | MS.gene71525:CDS | 40.0% | |
| TGTGGTTAAGAGCTATGACA+AGG | - | chr3.4:78284432-78284451 | MS.gene71525:CDS | 40.0% | |
| TTAAGAGCTATGACAAGGAG+AGG | - | chr3.4:78284437-78284456 | MS.gene71525:CDS | 40.0% | |
| TTACTTGATTCACTGCAGGA+GGG | + | chr3.4:78283942-78283961 | None:intergenic | 40.0% | |
| TTTGGCAGATTTGTCTTCGA+TGG | - | chr3.4:78284386-78284405 | MS.gene71525:CDS | 40.0% | |
| ! | AACCTTGTTTGATGGCTTCA+AGG | + | chr3.4:78283419-78283438 | None:intergenic | 40.0% |
| ! | AAGGTTGACTTCCACCTATT+AGG | + | chr3.4:78284074-78284093 | None:intergenic | 40.0% |
| ! | AGCCTCTTCTTGATGATCTA+TGG | - | chr3.4:78284560-78284579 | MS.gene71525:CDS | 40.0% |
| ! | CTGCAAGGAAAAGTGTATTG+TGG | - | chr3.4:78284123-78284142 | MS.gene71525:CDS | 40.0% |
| ! | CTTTGATTGGTCATTGACTG+AGG | - | chr3.4:78284480-78284499 | MS.gene71525:CDS | 40.0% |
| ! | GCCTCTTCTTGATGATCTAT+GGG | - | chr3.4:78284561-78284580 | MS.gene71525:CDS | 40.0% |
| !! | TGAGCAAGGATTGACTTTTG+TGG | - | chr3.4:78284414-78284433 | MS.gene71525:CDS | 40.0% |
| ACATGGCAAGACTATAGCTC+AGG | - | chr3.4:78284234-78284253 | MS.gene71525:intron | 45.0% | |
| CTGCTCTGCAGAAATCTCTA+AGG | - | chr3.4:78283595-78283614 | MS.gene71525:intron | 45.0% | |
| CTTAGAGATTTCTGCAGAGC+AGG | + | chr3.4:78283597-78283616 | None:intergenic | 45.0% | |
| CTTCGATGGCTATATGAGCA+AGG | - | chr3.4:78284400-78284419 | MS.gene71525:CDS | 45.0% | |
| GAAACTTAGAGCCTTCTGCA+AGG | - | chr3.4:78284108-78284127 | MS.gene71525:CDS | 45.0% | |
| GTCCTTGAAGCCATCAAACA+AGG | - | chr3.4:78283414-78283433 | MS.gene71525:CDS | 45.0% | |
| GTTTCTCTTGTTGCCATCCA+AGG | + | chr3.4:78284093-78284112 | None:intergenic | 45.0% | |
| TGAGAAGTTGCTAGCTCCAA+TGG | + | chr3.4:78283881-78283900 | None:intergenic | 45.0% | |
| TTTGATGCAGCTGCTGCATA+TGG | - | chr3.4:78283447-78283466 | MS.gene71525:CDS | 45.0% | |
| ! | GAAAGGTGTGTGGACATCAT+TGG | - | chr3.4:78283825-78283844 | MS.gene71525:intron | 45.0% |
| ! | GTAACCTTACTGGTTGCTTC+CGG | + | chr3.4:78283381-78283400 | None:intergenic | 45.0% |
| ! | TCATCAAGAAGAGGCTTGGT+TGG | + | chr3.4:78284556-78284575 | None:intergenic | 45.0% |
| !! | CAGGGGTGCTAATTTAGTGA+TGG | - | chr3.4:78284180-78284199 | MS.gene71525:intron | 45.0% |
| !!! | AATTATTGATTAAATTAATT+TGG | - | chr3.4:78284046-78284065 | MS.gene71525:CDS | 5.0% |
| ACAACGGAAAATGCCGGTGA+TGG | - | chr3.4:78283341-78283360 | MS.gene71525:CDS | 50.0% | |
| ACCTTTCCTAAGGGGTGAGA+AGG | + | chr3.4:78284155-78284174 | None:intergenic | 50.0% | |
| AGAGCAGGAACAATGAGCTC+AGG | + | chr3.4:78283582-78283601 | None:intergenic | 50.0% | |
| AGGACTACTTCAGGGACTGT+AGG | + | chr3.4:78283306-78283325 | None:intergenic | 50.0% | |
| AGGTGGAAGTCAACCTTGGA+TGG | - | chr3.4:78284077-78284096 | MS.gene71525:CDS | 50.0% | |
| CAACGGAAAATGCCGGTGAT+GGG | - | chr3.4:78283342-78283361 | MS.gene71525:CDS | 50.0% | |
| CCTGCTAGCACCTTTCCTAA+GGG | + | chr3.4:78284164-78284183 | None:intergenic | 50.0% | |
| CTGCTAGCACCTTTCCTAAG+GGG | + | chr3.4:78284163-78284182 | None:intergenic | 50.0% | |
| CTTGGTCTCACCAAAGCCAT+TGG | - | chr3.4:78283862-78283881 | MS.gene71525:intron | 50.0% | |
| GTTGCTAGCTCCAATGGCTT+TGG | + | chr3.4:78283875-78283894 | None:intergenic | 50.0% | |
| TAACTGCCTTCTCACCCCTT+AGG | - | chr3.4:78284146-78284165 | MS.gene71525:CDS | 50.0% | |
| TTGATTCACTGCAGGAGGGA+TGG | + | chr3.4:78283938-78283957 | None:intergenic | 50.0% | |
| !! | CCTTAGGAAAGGTGCTAGCA+GGG | - | chr3.4:78284162-78284181 | MS.gene71525:intron | 50.0% |
| !! | CTTAGGAAAGGTGCTAGCAG+GGG | - | chr3.4:78284163-78284182 | MS.gene71525:intron | 50.0% |
| AGGAGGGGAGGACTACTTCA+GGG | + | chr3.4:78283314-78283333 | None:intergenic | 55.0% | |
| CACCGGACAACGGAAAATGC+CGG | - | chr3.4:78283335-78283354 | MS.gene71525:CDS | 55.0% | |
| CCCTGCTAGCACCTTTCCTA+AGG | + | chr3.4:78284165-78284184 | None:intergenic | 55.0% | |
| GCCTTCTCACCCCTTAGGAA+AGG | - | chr3.4:78284151-78284170 | MS.gene71525:CDS | 55.0% | |
| TGCAGCTGCTGCATATGGTG+TGG | - | chr3.4:78283452-78283471 | MS.gene71525:CDS | 55.0% | |
| TGCAGGAGGGATGGTTGCAA+AGG | + | chr3.4:78283929-78283948 | None:intergenic | 55.0% | |
| ! | CACCGGCATTTTCCGTTGTC+CGG | + | chr3.4:78283340-78283359 | None:intergenic | 55.0% |
| ! | CATTTTCCGTTGTCCGGTGG+AGG | + | chr3.4:78283334-78283353 | None:intergenic | 55.0% |
| ! | CTTACTGGTTGCTTCCGGTG+CGG | + | chr3.4:78283376-78283395 | None:intergenic | 55.0% |
| !! | AAAATGCCGGTGATGGGCCT+CGG | - | chr3.4:78283348-78283367 | MS.gene71525:CDS | 55.0% |
| !! | CCCTTAGGAAAGGTGCTAGC+AGG | - | chr3.4:78284161-78284180 | MS.gene71525:intron | 55.0% |
| CGCACCGGAAGCAACCAGTA+AGG | - | chr3.4:78283374-78283393 | MS.gene71525:CDS | 60.0% | |
| GAGGAGGGGAGGACTACTTC+AGG | + | chr3.4:78283315-78283334 | None:intergenic | 60.0% | |
| TTCCGTTGTCCGGTGGAGGA+GGG | + | chr3.4:78283330-78283349 | None:intergenic | 60.0% | |
| TTTCCGTTGTCCGGTGGAGG+AGG | + | chr3.4:78283331-78283350 | None:intergenic | 60.0% | |
| ! | CGGCATTTTCCGTTGTCCGG+TGG | + | chr3.4:78283337-78283356 | None:intergenic | 60.0% |
| GTAGTCCTCCCCTCCTCCAC+CGG | - | chr3.4:78283318-78283337 | MS.gene71525:CDS | 65.0% | |
| TCCCCTCCTCCACCGGACAA+CGG | - | chr3.4:78283325-78283344 | MS.gene71525:CDS | 65.0% | |
| TCCGTTGTCCGGTGGAGGAG+GGG | + | chr3.4:78283329-78283348 | None:intergenic | 65.0% | |
| GTTGTCCGGTGGAGGAGGGG+AGG | + | chr3.4:78283326-78283345 | None:intergenic | 70.0% | |
| ! | TTGCTTCCGGTGCGGTGCCG+AGG | + | chr3.4:78283368-78283387 | None:intergenic | 70.0% |
| GATGGGCCTCGGCACCGCAC+CGG | - | chr3.4:78283359-78283378 | MS.gene71525:CDS | 75.0% | |
| ! | GCGGTGCCGAGGCCCATCAC+CGG | + | chr3.4:78283357-78283376 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 78283291 | 78284594 | 78283291 | ID=MS.gene71525 |
| chr3.4 | mRNA | 78283291 | 78284594 | 78283291 | ID=MS.gene71525.t1;Parent=MS.gene71525 |
| chr3.4 | exon | 78284269 | 78284594 | 78284269 | ID=MS.gene71525.t1.exon1;Parent=MS.gene71525.t1 |
| chr3.4 | CDS | 78284269 | 78284594 | 78284269 | ID=cds.MS.gene71525.t1;Parent=MS.gene71525.t1 |
| chr3.4 | exon | 78283928 | 78284171 | 78283928 | ID=MS.gene71525.t1.exon2;Parent=MS.gene71525.t1 |
| chr3.4 | CDS | 78283928 | 78284171 | 78283928 | ID=cds.MS.gene71525.t1;Parent=MS.gene71525.t1 |
| chr3.4 | exon | 78283630 | 78283806 | 78283630 | ID=MS.gene71525.t1.exon3;Parent=MS.gene71525.t1 |
| chr3.4 | CDS | 78283630 | 78283806 | 78283630 | ID=cds.MS.gene71525.t1;Parent=MS.gene71525.t1 |
| chr3.4 | exon | 78283291 | 78283491 | 78283291 | ID=MS.gene71525.t1.exon4;Parent=MS.gene71525.t1 |
| chr3.4 | CDS | 78283291 | 78283491 | 78283291 | ID=cds.MS.gene71525.t1;Parent=MS.gene71525.t1 |
| Gene Sequence |
| Protein sequence |
