Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77471.t1 | XP_003601570.1 | 99 | 315 | 3 | 0 | 1 | 315 | 1 | 315 | 2.70E-173 | 617.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77471.t1 | P26690 | 68.9 | 315 | 97 | 1 | 1 | 315 | 1 | 314 | 3.5e-129 | 462.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77471.t1 | G7J9I2 | 99.0 | 315 | 3 | 0 | 1 | 315 | 1 | 315 | 1.9e-173 | 617.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049232 | MS.gene77471 | -0.802501 | 5.45E-49 | -1.69E-46 |
| MS.gene049611 | MS.gene77471 | 0.801422 | 9.07E-49 | -1.69E-46 |
| MS.gene049634 | MS.gene77471 | 0.810458 | 1.14E-50 | -1.69E-46 |
| MS.gene050095 | MS.gene77471 | 0.813953 | 1.97E-51 | -1.69E-46 |
| MS.gene050096 | MS.gene77471 | 0.804905 | 1.73E-49 | -1.69E-46 |
| MS.gene050216 | MS.gene77471 | -0.821661 | 3.59E-53 | -1.69E-46 |
| MS.gene050363 | MS.gene77471 | 0.819405 | 1.18E-52 | -1.69E-46 |
| MS.gene050380 | MS.gene77471 | 0.869334 | 3.35E-66 | -1.69E-46 |
| MS.gene050381 | MS.gene77471 | 0.899542 | 1.79E-77 | -1.69E-46 |
| MS.gene050499 | MS.gene77471 | -0.802447 | 5.59E-49 | -1.69E-46 |
| MS.gene050502 | MS.gene77471 | -0.812334 | 4.47E-51 | -1.69E-46 |
| MS.gene050504 | MS.gene77471 | -0.800444 | 1.44E-48 | -1.69E-46 |
| MS.gene050662 | MS.gene77471 | -0.803438 | 3.49E-49 | -1.69E-46 |
| MS.gene050809 | MS.gene77471 | 0.80318 | 3.94E-49 | -1.69E-46 |
| MS.gene050868 | MS.gene77471 | -0.826073 | 3.31E-54 | -1.69E-46 |
| MS.gene050871 | MS.gene77471 | -0.830499 | 2.84E-55 | -1.69E-46 |
| MS.gene050883 | MS.gene77471 | 0.840965 | 6.33E-58 | -1.69E-46 |
| MS.gene05104 | MS.gene77471 | 0.815579 | 8.60E-52 | -1.69E-46 |
| MS.gene051127 | MS.gene77471 | 0.851348 | 9.41E-61 | -1.69E-46 |
| MS.gene05141 | MS.gene77471 | 0.813838 | 2.09E-51 | -1.69E-46 |
| MS.gene051509 | MS.gene77471 | -0.807228 | 5.60E-50 | -1.69E-46 |
| MS.gene051946 | MS.gene77471 | 0.824748 | 6.82E-54 | -1.69E-46 |
| MS.gene052164 | MS.gene77471 | -0.813304 | 2.74E-51 | -1.69E-46 |
| MS.gene05240 | MS.gene77471 | 0.800594 | 1.34E-48 | -1.69E-46 |
| MS.gene052966 | MS.gene77471 | -0.803838 | 2.88E-49 | -1.69E-46 |
| MS.gene053018 | MS.gene77471 | -0.814652 | 1.38E-51 | -1.69E-46 |
| MS.gene053031 | MS.gene77471 | 0.802166 | 6.38E-49 | -1.69E-46 |
| MS.gene053203 | MS.gene77471 | -0.802293 | 6.01E-49 | -1.69E-46 |
| MS.gene053319 | MS.gene77471 | 0.822621 | 2.15E-53 | -1.69E-46 |
| MS.gene053406 | MS.gene77471 | 0.810479 | 1.13E-50 | -1.69E-46 |
| MS.gene053420 | MS.gene77471 | 0.832615 | 8.54E-56 | -1.69E-46 |
| MS.gene053436 | MS.gene77471 | -0.812123 | 4.97E-51 | -1.69E-46 |
| MS.gene053488 | MS.gene77471 | 0.825966 | 3.51E-54 | -1.69E-46 |
| MS.gene053561 | MS.gene77471 | 0.837589 | 4.75E-57 | -1.69E-46 |
| MS.gene053574 | MS.gene77471 | -0.825586 | 4.32E-54 | -1.69E-46 |
| MS.gene053626 | MS.gene77471 | -0.805424 | 1.34E-49 | -1.69E-46 |
| MS.gene054003 | MS.gene77471 | -0.81155 | 6.62E-51 | -1.69E-46 |
| MS.gene054595 | MS.gene77471 | -0.827752 | 1.32E-54 | -1.69E-46 |
| MS.gene054706 | MS.gene77471 | 0.807182 | 5.73E-50 | -1.69E-46 |
| MS.gene054708 | MS.gene77471 | 0.827542 | 1.48E-54 | -1.69E-46 |
| MS.gene055124 | MS.gene77471 | 0.883518 | 4.19E-71 | -1.69E-46 |
| MS.gene055139 | MS.gene77471 | 0.809954 | 1.47E-50 | -1.69E-46 |
| MS.gene055154 | MS.gene77471 | 0.856203 | 3.77E-62 | -1.69E-46 |
| MS.gene055202 | MS.gene77471 | 0.813361 | 2.66E-51 | -1.69E-46 |
| MS.gene055401 | MS.gene77471 | 0.824223 | 9.07E-54 | -1.69E-46 |
| MS.gene055502 | MS.gene77471 | -0.800065 | 1.72E-48 | -1.69E-46 |
| MS.gene055577 | MS.gene77471 | -0.807461 | 5.00E-50 | -1.69E-46 |
| MS.gene055959 | MS.gene77471 | 0.825253 | 5.18E-54 | -1.69E-46 |
| MS.gene056166 | MS.gene77471 | -0.821192 | 4.60E-53 | -1.69E-46 |
| MS.gene056310 | MS.gene77471 | 0.880357 | 5.87E-70 | -1.69E-46 |
| MS.gene056311 | MS.gene77471 | 0.846887 | 1.64E-59 | -1.69E-46 |
| MS.gene056313 | MS.gene77471 | 0.912017 | 3.15E-83 | -1.69E-46 |
| MS.gene056408 | MS.gene77471 | 0.821791 | 3.35E-53 | -1.69E-46 |
| MS.gene056422 | MS.gene77471 | -0.848712 | 5.15E-60 | -1.69E-46 |
| MS.gene056448 | MS.gene77471 | -0.810373 | 1.19E-50 | -1.69E-46 |
| MS.gene057014 | MS.gene77471 | 0.8101 | 1.36E-50 | -1.69E-46 |
| MS.gene057086 | MS.gene77471 | -0.814965 | 1.18E-51 | -1.69E-46 |
| MS.gene058093 | MS.gene77471 | 0.823834 | 1.12E-53 | -1.69E-46 |
| MS.gene058099 | MS.gene77471 | 0.835851 | 1.32E-56 | -1.69E-46 |
| MS.gene058108 | MS.gene77471 | 0.853402 | 2.45E-61 | -1.69E-46 |
| MS.gene058109 | MS.gene77471 | 0.859269 | 4.65E-63 | -1.69E-46 |
| MS.gene058170 | MS.gene77471 | 0.825245 | 5.21E-54 | -1.69E-46 |
| MS.gene058171 | MS.gene77471 | 0.863654 | 2.13E-64 | -1.69E-46 |
| MS.gene058173 | MS.gene77471 | 0.855731 | 5.18E-62 | -1.69E-46 |
| MS.gene058174 | MS.gene77471 | 0.826959 | 2.04E-54 | -1.69E-46 |
| MS.gene058175 | MS.gene77471 | 0.862898 | 3.66E-64 | -1.69E-46 |
| MS.gene058176 | MS.gene77471 | 0.829483 | 5.02E-55 | -1.69E-46 |
| MS.gene058179 | MS.gene77471 | 0.864395 | 1.25E-64 | -1.69E-46 |
| MS.gene058180 | MS.gene77471 | 0.857878 | 1.21E-62 | -1.69E-46 |
| MS.gene058268 | MS.gene77471 | 0.801218 | 9.99E-49 | -1.69E-46 |
| MS.gene058269 | MS.gene77471 | 0.809878 | 1.52E-50 | -1.69E-46 |
| MS.gene05837 | MS.gene77471 | -0.829277 | 5.63E-55 | -1.69E-46 |
| MS.gene058840 | MS.gene77471 | 0.856996 | 2.20E-62 | -1.69E-46 |
| MS.gene059037 | MS.gene77471 | 0.871396 | 7.06E-67 | -1.69E-46 |
| MS.gene059038 | MS.gene77471 | 0.824935 | 6.16E-54 | -1.69E-46 |
| MS.gene059044 | MS.gene77471 | 0.824143 | 9.47E-54 | -1.69E-46 |
| MS.gene059045 | MS.gene77471 | 0.845083 | 5.07E-59 | -1.69E-46 |
| MS.gene059223 | MS.gene77471 | 0.807868 | 4.10E-50 | -1.69E-46 |
| MS.gene059367 | MS.gene77471 | -0.839754 | 1.31E-57 | -1.69E-46 |
| MS.gene059368 | MS.gene77471 | -0.827412 | 1.59E-54 | -1.69E-46 |
| MS.gene059517 | MS.gene77471 | -0.819438 | 1.16E-52 | -1.69E-46 |
| MS.gene059680 | MS.gene77471 | -0.803447 | 3.47E-49 | -1.69E-46 |
| MS.gene059690 | MS.gene77471 | 0.824707 | 6.98E-54 | -1.69E-46 |
| MS.gene059691 | MS.gene77471 | 0.836045 | 1.18E-56 | -1.69E-46 |
| MS.gene059694 | MS.gene77471 | 0.838081 | 3.55E-57 | -1.69E-46 |
| MS.gene059697 | MS.gene77471 | 0.857014 | 2.18E-62 | -1.69E-46 |
| MS.gene059865 | MS.gene77471 | -0.808052 | 3.74E-50 | -1.69E-46 |
| MS.gene059916 | MS.gene77471 | 0.804344 | 2.26E-49 | -1.69E-46 |
| MS.gene059943 | MS.gene77471 | 0.812063 | 5.12E-51 | -1.69E-46 |
| MS.gene059944 | MS.gene77471 | 0.842459 | 2.55E-58 | -1.69E-46 |
| MS.gene060482 | MS.gene77471 | -0.856028 | 4.24E-62 | -1.69E-46 |
| MS.gene06074 | MS.gene77471 | 0.803369 | 3.60E-49 | -1.69E-46 |
| MS.gene06077 | MS.gene77471 | -0.805552 | 1.26E-49 | -1.69E-46 |
| MS.gene060808 | MS.gene77471 | 0.834849 | 2.36E-56 | -1.69E-46 |
| MS.gene060837 | MS.gene77471 | -0.817007 | 4.12E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene72560 | MS.gene77471 | PPI |
| MS.gene008793 | MS.gene77471 | PPI |
| MS.gene53169 | MS.gene77471 | PPI |
| MS.gene93058 | MS.gene77471 | PPI |
| MS.gene62620 | MS.gene77471 | PPI |
| MS.gene056488 | MS.gene77471 | PPI |
| MS.gene77471 | MS.gene024362 | PPI |
| MS.gene97981 | MS.gene77471 | PPI |
| MS.gene87111 | MS.gene77471 | PPI |
| MS.gene93059 | MS.gene77471 | PPI |
| MS.gene99693 | MS.gene77471 | PPI |
| MS.gene77471 | MS.gene93783 | PPI |
| MS.gene55382 | MS.gene77471 | PPI |
| MS.gene040151 | MS.gene77471 | PPI |
| MS.gene062932 | MS.gene77471 | PPI |
| MS.gene54933 | MS.gene77471 | PPI |
| MS.gene95889 | MS.gene77471 | PPI |
| MS.gene056487 | MS.gene77471 | PPI |
| MS.gene50700 | MS.gene77471 | PPI |
| MS.gene23359 | MS.gene77471 | PPI |
| MS.gene42146 | MS.gene77471 | PPI |
| MS.gene77471 | MS.gene97980 | PPI |
| MS.gene42873 | MS.gene77471 | PPI |
| MS.gene003638 | MS.gene77471 | PPI |
| MS.gene98697 | MS.gene77471 | PPI |
| MS.gene008794 | MS.gene77471 | PPI |
| MS.gene91446 | MS.gene77471 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77471.t1 | MTR_3g083130 | 99.048 | 315 | 3 | 0 | 1 | 315 | 1 | 315 | 0.0 | 637 |
| MS.gene77471.t1 | MTR_5g097900 | 68.506 | 308 | 96 | 1 | 7 | 314 | 6 | 312 | 4.86e-154 | 434 |
| MS.gene77471.t1 | MTR_5g097910 | 68.182 | 308 | 97 | 1 | 7 | 314 | 6 | 312 | 1.12e-153 | 433 |
| MS.gene77471.t1 | MTR_5g097940 | 68.182 | 308 | 97 | 1 | 7 | 314 | 6 | 312 | 1.12e-153 | 433 |
| MS.gene77471.t1 | MTR_0374s0050 | 62.857 | 315 | 114 | 3 | 3 | 315 | 2 | 315 | 5.74e-143 | 406 |
| MS.gene77471.t1 | MTR_4g072320 | 50.633 | 316 | 138 | 5 | 7 | 313 | 6 | 312 | 2.05e-106 | 313 |
| MS.gene77471.t1 | MTR_6g073110 | 51.325 | 302 | 136 | 4 | 6 | 302 | 5 | 300 | 1.09e-103 | 306 |
| MS.gene77471.t1 | MTR_4g072350 | 49.686 | 318 | 144 | 6 | 7 | 313 | 6 | 318 | 1.86e-103 | 306 |
| MS.gene77471.t1 | MTR_4g072360 | 48.910 | 321 | 148 | 6 | 4 | 313 | 3 | 318 | 1.46e-102 | 303 |
| MS.gene77471.t1 | MTR_4g036845 | 40.328 | 305 | 168 | 7 | 21 | 315 | 14 | 314 | 6.42e-75 | 233 |
| MS.gene77471.t1 | MTR_8g070095 | 40.702 | 285 | 165 | 3 | 20 | 302 | 15 | 297 | 7.36e-71 | 223 |
| MS.gene77471.t1 | MTR_4g021350 | 45.230 | 283 | 149 | 5 | 20 | 302 | 15 | 291 | 1.91e-69 | 219 |
| MS.gene77471.t1 | MTR_8g070115 | 40.656 | 305 | 167 | 5 | 20 | 315 | 15 | 314 | 8.52e-68 | 214 |
| MS.gene77471.t1 | MTR_7g063580 | 41.311 | 305 | 164 | 5 | 20 | 315 | 55 | 353 | 6.65e-67 | 213 |
| MS.gene77471.t1 | MTR_4g021350 | 44.765 | 277 | 147 | 5 | 20 | 296 | 15 | 285 | 1.28e-66 | 211 |
| MS.gene77471.t1 | MTR_4g021410 | 41.118 | 304 | 156 | 5 | 20 | 315 | 16 | 304 | 1.48e-64 | 206 |
| MS.gene77471.t1 | MTR_7g070500 | 37.134 | 307 | 172 | 7 | 21 | 315 | 23 | 320 | 1.31e-62 | 201 |
| MS.gene77471.t1 | MTR_4g021450 | 40.461 | 304 | 158 | 5 | 20 | 315 | 16 | 304 | 1.26e-61 | 198 |
| MS.gene77471.t1 | MTR_4g021410 | 41.455 | 275 | 140 | 4 | 49 | 315 | 6 | 267 | 1.76e-60 | 194 |
| MS.gene77471.t1 | MTR_7g070500 | 38.545 | 275 | 160 | 5 | 21 | 294 | 23 | 289 | 3.89e-60 | 194 |
| MS.gene77471.t1 | MTR_4g072060 | 37.171 | 304 | 175 | 6 | 20 | 312 | 10 | 308 | 1.64e-52 | 175 |
| MS.gene77471.t1 | MTR_4g021410 | 41.250 | 240 | 121 | 3 | 84 | 315 | 1 | 228 | 2.83e-49 | 164 |
| MS.gene77471.t1 | MTR_4g021450 | 40.833 | 240 | 122 | 3 | 84 | 315 | 1 | 228 | 2.74e-47 | 159 |
| MS.gene77471.t1 | MTR_4g021450 | 39.130 | 184 | 101 | 2 | 140 | 315 | 5 | 185 | 1.61e-39 | 137 |
| MS.gene77471.t1 | MTR_6g072650 | 45.333 | 150 | 46 | 5 | 175 | 313 | 10 | 134 | 7.96e-29 | 108 |
| MS.gene77471.t1 | MTR_6g072710 | 47.788 | 113 | 54 | 2 | 193 | 302 | 1 | 111 | 1.17e-26 | 101 |
| MS.gene77471.t1 | MTR_3g449790 | 25.000 | 308 | 162 | 10 | 39 | 308 | 77 | 353 | 2.15e-12 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77471.t1 | AT1G59960 | 51.692 | 325 | 138 | 7 | 6 | 315 | 5 | 325 | 5.18e-114 | 333 |
| MS.gene77471.t1 | AT1G59950 | 52.681 | 317 | 138 | 5 | 10 | 315 | 4 | 319 | 7.11e-109 | 320 |
| MS.gene77471.t1 | AT5G01670 | 40.645 | 310 | 159 | 9 | 20 | 315 | 23 | 321 | 3.74e-73 | 228 |
| MS.gene77471.t1 | AT2G37790 | 42.105 | 304 | 163 | 6 | 20 | 315 | 15 | 313 | 4.85e-72 | 225 |
| MS.gene77471.t1 | AT2G37770 | 41.503 | 306 | 163 | 6 | 20 | 315 | 15 | 314 | 1.62e-71 | 224 |
| MS.gene77471.t1 | AT5G62420 | 39.869 | 306 | 169 | 7 | 21 | 315 | 14 | 315 | 7.94e-71 | 222 |
| MS.gene77471.t1 | AT5G01670 | 37.389 | 337 | 159 | 10 | 20 | 315 | 23 | 348 | 3.24e-67 | 214 |
| MS.gene77471.t1 | AT2G37760 | 39.016 | 305 | 168 | 5 | 20 | 315 | 15 | 310 | 2.21e-66 | 211 |
| MS.gene77471.t1 | AT2G37760 | 39.016 | 305 | 168 | 5 | 20 | 315 | 15 | 310 | 2.21e-66 | 211 |
| MS.gene77471.t1 | AT2G37760 | 41.071 | 280 | 155 | 4 | 20 | 298 | 15 | 285 | 4.05e-66 | 209 |
| MS.gene77471.t1 | AT2G37760 | 40.426 | 282 | 158 | 4 | 20 | 300 | 15 | 287 | 6.97e-66 | 209 |
| MS.gene77471.t1 | AT2G37760 | 40.426 | 282 | 158 | 4 | 20 | 300 | 15 | 287 | 6.97e-66 | 209 |
| MS.gene77471.t1 | AT3G53880 | 37.908 | 306 | 174 | 6 | 20 | 315 | 15 | 314 | 2.21e-62 | 201 |
| MS.gene77471.t1 | AT3G53880 | 38.129 | 278 | 166 | 4 | 20 | 296 | 15 | 287 | 2.36e-58 | 190 |
| MS.gene77471.t1 | AT2G21250 | 34.539 | 304 | 183 | 6 | 20 | 312 | 10 | 308 | 1.31e-57 | 188 |
| MS.gene77471.t1 | AT2G21260 | 35.417 | 288 | 170 | 6 | 20 | 296 | 10 | 292 | 7.85e-57 | 186 |
| MS.gene77471.t1 | AT2G37760 | 38.168 | 262 | 150 | 4 | 63 | 315 | 6 | 264 | 4.37e-54 | 177 |
| MS.gene77471.t1 | AT2G37760 | 38.168 | 262 | 150 | 4 | 63 | 315 | 6 | 264 | 4.37e-54 | 177 |
| MS.gene77471.t1 | AT2G37770 | 45.833 | 192 | 99 | 3 | 20 | 211 | 15 | 201 | 3.24e-48 | 163 |
| MS.gene77471.t1 | AT2G21250 | 36.681 | 229 | 129 | 6 | 20 | 237 | 10 | 233 | 4.54e-43 | 148 |
| MS.gene77471.t1 | AT2G21260 | 31.797 | 217 | 135 | 4 | 89 | 294 | 8 | 222 | 4.94e-35 | 127 |
| MS.gene77471.t1 | AT1G06690 | 25.763 | 295 | 166 | 7 | 39 | 297 | 88 | 365 | 2.13e-17 | 82.4 |
| MS.gene77471.t1 | AT5G53580 | 25.267 | 281 | 154 | 12 | 45 | 287 | 80 | 342 | 5.96e-12 | 66.2 |
Find 82 sgRNAs with CRISPR-Local
Find 101 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTTGTTACTTCTAAATTA+TGG | 0.129563 | 3.2:-72001397 | MS.gene77471:CDS |
| AGCCTCTTCTTGATGATCTA+TGG | 0.232119 | 3.2:-72000378 | MS.gene77471:CDS |
| CTTTGATGAGTCTCTCTTGA+TGG | 0.239320 | 3.2:+72000410 | None:intergenic |
| AATCAAAGATTTGCAAGTTT+TGG | 0.245163 | 3.2:+72000473 | None:intergenic |
| TCACCTGGTTTAGTAGTAAT+TGG | 0.253992 | 3.2:+72001181 | None:intergenic |
| CTTTGTTACTTCTAAATTAT+GGG | 0.275213 | 3.2:-72001396 | MS.gene77471:CDS |
| ATGTCTATAACCTTGTTTGA+TGG | 0.330198 | 3.2:+72001514 | None:intergenic |
| ACAATCTCTGATACTTCAAT+AGG | 0.347637 | 3.2:+72001148 | None:intergenic |
| AAAACTTGCAAATCTTTGAT+TGG | 0.362637 | 3.2:-72000471 | MS.gene77471:CDS |
| AACCTTGTTTGATGGCTTCA+AGG | 0.381003 | 3.2:+72001522 | None:intergenic |
| CCCTGCTAGCACCTTTCCTA+AGG | 0.387480 | 3.2:+72000776 | None:intergenic |
| CAATGATATACTCAAAGAAT+TGG | 0.390203 | 3.2:-72000734 | MS.gene77471:CDS |
| GTAACCTTACTGGTTGCTTC+CGG | 0.403522 | 3.2:+72001560 | None:intergenic |
| GTTACTAGCTCCAATGGCTT+TGG | 0.423894 | 3.2:+72001066 | None:intergenic |
| TTTCCGTTGTCCGGTGGAGG+AGG | 0.424568 | 3.2:+72001610 | None:intergenic |
| TTGGCCAATGGATCAAAATA+AGG | 0.425035 | 3.2:+72001200 | None:intergenic |
| CTTAGAGATTTCTGCAGAGC+AGG | 0.429110 | 3.2:+72001344 | None:intergenic |
| CTGCTCTGCAGAAATCTCTA+AGG | 0.431594 | 3.2:-72001343 | MS.gene77471:intron |
| CCCTTAGGAAAGGTGCTAGC+AGG | 0.441076 | 3.2:-72000777 | MS.gene77471:CDS |
| GAGGAGGGGAGGACTACTTC+AGG | 0.445285 | 3.2:+72001626 | None:intergenic |
| CAATCTCTGATACTTCAATA+GGG | 0.455333 | 3.2:+72001149 | None:intergenic |
| GTTTCTCTTGTTGCCATCCA+AGG | 0.461119 | 3.2:+72000848 | None:intergenic |
| TTCCGTTGTCCGGTGGAGGA+GGG | 0.463761 | 3.2:+72001611 | None:intergenic |
| GACTCTTCAACTAGAATACT+TGG | 0.468397 | 3.2:-72001224 | MS.gene77471:CDS |
| AAAATGCCGGTGATGGGCCT+CGG | 0.468907 | 3.2:-72001590 | MS.gene77471:CDS |
| TTTGATGCAGCTGCTGCATA+TGG | 0.474976 | 3.2:-72001491 | MS.gene77471:CDS |
| TCCGTTGTCCGGTGGAGGAG+GGG | 0.476698 | 3.2:+72001612 | None:intergenic |
| GCCTCTTCTTGATGATCTAT+GGG | 0.483427 | 3.2:-72000377 | MS.gene77471:CDS |
| CCTGCTAGCACCTTTCCTAA+GGG | 0.491638 | 3.2:+72000777 | None:intergenic |
| ACAACGGAAAATGCCGGTGA+TGG | 0.497720 | 3.2:-72001597 | MS.gene77471:CDS |
| CTTGGTCTCACCAAAGCCAT+TGG | 0.503945 | 3.2:-72001076 | MS.gene77471:CDS |
| TCAATTACTTGATTCACTGC+AGG | 0.507920 | 3.2:+72000995 | None:intergenic |
| TTTGGCAGATTTGTCTTCGA+TGG | 0.511955 | 3.2:-72000552 | MS.gene77471:intron |
| GAAAGGTGTGTGGACATCAT+TGG | 0.513831 | 3.2:-72001113 | MS.gene77471:CDS |
| ATTGCAGAAGCACTTAAACT+TGG | 0.516828 | 3.2:-72001443 | MS.gene77471:CDS |
| CAACGGAAAATGCCGGTGAT+GGG | 0.522929 | 3.2:-72001596 | MS.gene77471:CDS |
| TAACTGCCTTCTCACCCCTT+AGG | 0.527768 | 3.2:-72000792 | MS.gene77471:CDS |
| CCTTAGGAAAGGTGCTAGCA+GGG | 0.529822 | 3.2:-72000776 | MS.gene77471:CDS |
| ATAGGGTATTTAACTTCACC+TGG | 0.535676 | 3.2:+72001166 | None:intergenic |
| TTGCTTCCGGTGCGGTGCCG+AGG | 0.538965 | 3.2:+72001573 | None:intergenic |
| TGAGAAGTTACTAGCTCCAA+TGG | 0.540044 | 3.2:+72001060 | None:intergenic |
| GTAGTCCTCCCCTCCTCCAC+CGG | 0.540364 | 3.2:-72001620 | MS.gene77471:CDS |
| TTGATTCACTGCAGGAGGGA+TGG | 0.540989 | 3.2:+72001003 | None:intergenic |
| ACCTTTCCTAAGGGGTGAGA+AGG | 0.541355 | 3.2:+72000786 | None:intergenic |
| GTTGTCCGGTGGAGGAGGGG+AGG | 0.547684 | 3.2:+72001615 | None:intergenic |
| AGGTGGAAGTCAACCTTGGA+TGG | 0.547815 | 3.2:-72000861 | MS.gene77471:CDS |
| GATGGGCCTCGGCACCGCAC+CGG | 0.552144 | 3.2:-72001579 | MS.gene77471:CDS |
| TATTGAAGTATCAGAGATTG+TGG | 0.554195 | 3.2:-72001146 | MS.gene77471:CDS |
| AGGACTACTTCAGGGACTGT+AGG | 0.556376 | 3.2:+72001635 | None:intergenic |
| TAGATCATCAAGAAGAGGCT+TGG | 0.556426 | 3.2:+72000381 | None:intergenic |
| TCATCAAGAAGAGGCTTGGT+TGG | 0.556818 | 3.2:+72000385 | None:intergenic |
| AGAGCAGGAACAATGAGCTC+AGG | 0.564156 | 3.2:+72001359 | None:intergenic |
| ATTACTTGATTCACTGCAGG+AGG | 0.566545 | 3.2:+72000998 | None:intergenic |
| TCCCCTCCTCCACCGGACAA+CGG | 0.566967 | 3.2:-72001613 | MS.gene77471:CDS |
| GCGGTGCCGAGGCCCATCAC+CGG | 0.568831 | 3.2:+72001584 | None:intergenic |
| TAATAGGTGGAAGTCAACCT+TGG | 0.576953 | 3.2:-72000865 | MS.gene77471:intron |
| GTTTAGTAGTAATTGGCCAA+TGG | 0.578679 | 3.2:+72001188 | None:intergenic |
| TGCAGGAGGGATGGTTGCAA+AGG | 0.578968 | 3.2:+72001012 | None:intergenic |
| TCCCATAGATCATCAAGAAG+AGG | 0.583002 | 3.2:+72000376 | None:intergenic |
| CGCACCGGAAGCAACCAGTA+AGG | 0.584380 | 3.2:-72001564 | MS.gene77471:CDS |
| ACATGGCAAGACTATAGCTC+AGG | 0.585566 | 3.2:-72000704 | MS.gene77471:intron |
| CTTCGATGGCTATATGAGCA+AGG | 0.590577 | 3.2:-72000538 | MS.gene77471:CDS |
| GTCCTTGAAGCCATCAAACA+AGG | 0.591634 | 3.2:-72001524 | MS.gene77471:CDS |
| CACCGGACAACGGAAAATGC+CGG | 0.597803 | 3.2:-72001603 | MS.gene77471:CDS |
| CAGGGGTGCTAATTTAGTGA+TGG | 0.600153 | 3.2:-72000758 | MS.gene77471:CDS |
| AGGAGGGGAGGACTACTTCA+GGG | 0.602610 | 3.2:+72001627 | None:intergenic |
| TTAAGAGCTATGACAAGGAG+AGG | 0.604664 | 3.2:-72000501 | MS.gene77471:CDS |
| GCCTTCTCACCCCTTAGGAA+AGG | 0.609336 | 3.2:-72000787 | MS.gene77471:CDS |
| AAAGAATTGGCTGATGCACA+TGG | 0.613616 | 3.2:-72000721 | MS.gene77471:CDS |
| CTTTGATTGGTCATTGACTG+AGG | 0.619859 | 3.2:-72000458 | MS.gene77471:CDS |
| CTTACTGGTTGCTTCCGGTG+CGG | 0.628936 | 3.2:+72001565 | None:intergenic |
| CTGCTAGCACCTTTCCTAAG+GGG | 0.635830 | 3.2:+72000778 | None:intergenic |
| TGTGGTTAAGAGCTATGACA+AGG | 0.641038 | 3.2:-72000506 | MS.gene77471:CDS |
| CATCAAGAGAGACTCATCAA+AGG | 0.641861 | 3.2:-72000409 | MS.gene77471:CDS |
| TGGCCAATTACTACTAAACC+AGG | 0.647399 | 3.2:-72001184 | MS.gene77471:CDS |
| ATTGTGGAATTTGATATGAA+AGG | 0.649138 | 3.2:-72001130 | MS.gene77471:CDS |
| GAAACTTAGAGCCTTCTGCA+AGG | 0.650710 | 3.2:-72000830 | MS.gene77471:CDS |
| CTTAGGAAAGGTGCTAGCAG+GGG | 0.654145 | 3.2:-72000775 | MS.gene77471:CDS |
| CTGCAAGGAAAAGTGTATTG+TGG | 0.655539 | 3.2:-72000815 | MS.gene77471:CDS |
| TTACTTGATTCACTGCAGGA+GGG | 0.664815 | 3.2:+72000999 | None:intergenic |
| AATTTGATATGAAAGGTGTG+TGG | 0.691006 | 3.2:-72001123 | MS.gene77471:CDS |
| TGCAGCTGCTGCATATGGTG+TGG | 0.691514 | 3.2:-72001486 | MS.gene77471:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTAATGTCACAATACTT+TGG | - | chr3.2:72001443-72001462 | MS.gene77471:CDS | 20.0% |
| !! | ACATATGAAATCATGTAAAA+TGG | + | chr3.2:72001078-72001097 | None:intergenic | 20.0% |
| !! | TATATAATATGATCTTAGCA+TGG | - | chr3.2:72001341-72001360 | MS.gene77471:intron | 20.0% |
| !!! | AAATTAATTTGGATCCTAAT+AGG | - | chr3.2:72001132-72001151 | MS.gene77471:CDS | 20.0% |
| !!! | CTTTGTTACTTCTAAATTAT+GGG | - | chr3.2:72000617-72000636 | MS.gene77471:intron | 20.0% |
| !!! | TCTTTGTTACTTCTAAATTA+TGG | - | chr3.2:72000616-72000635 | MS.gene77471:intron | 20.0% |
| ! | AAAACTTGCAAATCTTTGAT+TGG | - | chr3.2:72001542-72001561 | MS.gene77471:CDS | 25.0% |
| ! | ATTGTGGAATTTGATATGAA+AGG | - | chr3.2:72000883-72000902 | MS.gene77471:intron | 25.0% |
| ! | CAATGATATACTCAAAGAAT+TGG | - | chr3.2:72001279-72001298 | MS.gene77471:intron | 25.0% |
| !! | AATCAAAGATTTGCAAGTTT+TGG | + | chr3.2:72001543-72001562 | None:intergenic | 25.0% |
| AATCACTCCAAATTAGAAGT+TGG | + | chr3.2:72001368-72001387 | None:intergenic | 30.0% | |
| ACAATCTCTGATACTTCAAT+AGG | + | chr3.2:72000868-72000887 | None:intergenic | 30.0% | |
| CAATCTCTGATACTTCAATA+GGG | + | chr3.2:72000867-72000886 | None:intergenic | 30.0% | |
| TTGGAAGAATGTCAAAAACT+TGG | - | chr3.2:72000919-72000938 | MS.gene77471:intron | 30.0% | |
| ! | AATTTGATATGAAAGGTGTG+TGG | - | chr3.2:72000890-72000909 | MS.gene77471:intron | 30.0% |
| ! | ATCTTTTGTAGTAACCTTAC+TGG | + | chr3.2:72000466-72000485 | None:intergenic | 30.0% |
| ! | ATGTCTATAACCTTGTTTGA+TGG | + | chr3.2:72000502-72000521 | None:intergenic | 30.0% |
| ! | TATTGAAGTATCAGAGATTG+TGG | - | chr3.2:72000867-72000886 | MS.gene77471:intron | 30.0% |
| ! | TTAATTTGGATCCTAATAGG+TGG | - | chr3.2:72001135-72001154 | MS.gene77471:CDS | 30.0% |
| GACTCTTCAACTAGAATACT+TGG | - | chr3.2:72000789-72000808 | MS.gene77471:CDS | 35.0% | |
| GTTTAGTAGTAATTGGCCAA+TGG | + | chr3.2:72000828-72000847 | None:intergenic | 35.0% | |
| TATGGTGTGGAAAAATCTGT+TGG | - | chr3.2:72000540-72000559 | MS.gene77471:CDS | 35.0% | |
| TCAATTACTTGATTCACTGC+AGG | + | chr3.2:72001021-72001040 | None:intergenic | 35.0% | |
| TCACCTGGTTTAGTAGTAAT+TGG | + | chr3.2:72000835-72000854 | None:intergenic | 35.0% | |
| TTGGCCAATGGATCAAAATA+AGG | + | chr3.2:72000816-72000835 | None:intergenic | 35.0% | |
| ! | AATACACTTTTCCTTGCAGA+AGG | + | chr3.2:72001197-72001216 | None:intergenic | 35.0% |
| ! | ATAGGGTATTTAACTTCACC+TGG | + | chr3.2:72000850-72000869 | None:intergenic | 35.0% |
| !! | ATTGCAGAAGCACTTAAACT+TGG | - | chr3.2:72000570-72000589 | MS.gene77471:intron | 35.0% |
| !!! | GTATACATGCTTGTTTTTGC+AGG | - | chr3.2:72000767-72000786 | MS.gene77471:CDS | 35.0% |
| !!! | TGGACCTTATTTTGATCCAT+TGG | - | chr3.2:72000809-72000828 | MS.gene77471:CDS | 35.0% |
| AAAGAATTGGCTGATGCACA+TGG | - | chr3.2:72001292-72001311 | MS.gene77471:intron | 40.0% | |
| ATTACTTGATTCACTGCAGG+AGG | + | chr3.2:72001018-72001037 | None:intergenic | 40.0% | |
| CATCAAGAGAGACTCATCAA+AGG | - | chr3.2:72001604-72001623 | MS.gene77471:CDS | 40.0% | |
| CTTTGATGAGTCTCTCTTGA+TGG | + | chr3.2:72001606-72001625 | None:intergenic | 40.0% | |
| GCATGGACCAACTTCTAATT+TGG | - | chr3.2:72001358-72001377 | MS.gene77471:CDS | 40.0% | |
| TAATAGGTGGAAGTCAACCT+TGG | - | chr3.2:72001148-72001167 | MS.gene77471:CDS | 40.0% | |
| TAGATCATCAAGAAGAGGCT+TGG | + | chr3.2:72001635-72001654 | None:intergenic | 40.0% | |
| TCCCATAGATCATCAAGAAG+AGG | + | chr3.2:72001640-72001659 | None:intergenic | 40.0% | |
| TGAGAAGTTACTAGCTCCAA+TGG | + | chr3.2:72000956-72000975 | None:intergenic | 40.0% | |
| TGGCCAATTACTACTAAACC+AGG | - | chr3.2:72000829-72000848 | MS.gene77471:CDS | 40.0% | |
| TGTGGTTAAGAGCTATGACA+AGG | - | chr3.2:72001507-72001526 | MS.gene77471:CDS | 40.0% | |
| TTAAGAGCTATGACAAGGAG+AGG | - | chr3.2:72001512-72001531 | MS.gene77471:CDS | 40.0% | |
| TTACTTGATTCACTGCAGGA+GGG | + | chr3.2:72001017-72001036 | None:intergenic | 40.0% | |
| TTTGGCAGATTTGTCTTCGA+TGG | - | chr3.2:72001461-72001480 | MS.gene77471:CDS | 40.0% | |
| ! | AACCTTGTTTGATGGCTTCA+AGG | + | chr3.2:72000494-72000513 | None:intergenic | 40.0% |
| ! | AAGGTTGACTTCCACCTATT+AGG | + | chr3.2:72001149-72001168 | None:intergenic | 40.0% |
| ! | AGCCTCTTCTTGATGATCTA+TGG | - | chr3.2:72001635-72001654 | MS.gene77471:CDS | 40.0% |
| ! | CTGCAAGGAAAAGTGTATTG+TGG | - | chr3.2:72001198-72001217 | MS.gene77471:CDS | 40.0% |
| ! | CTTTGATTGGTCATTGACTG+AGG | - | chr3.2:72001555-72001574 | MS.gene77471:CDS | 40.0% |
| ! | GCCTCTTCTTGATGATCTAT+GGG | - | chr3.2:72001636-72001655 | MS.gene77471:CDS | 40.0% |
| !! | TGAGCAAGGATTGACTTTTG+TGG | - | chr3.2:72001489-72001508 | MS.gene77471:CDS | 40.0% |
| ACATGGCAAGACTATAGCTC+AGG | - | chr3.2:72001309-72001328 | MS.gene77471:intron | 45.0% | |
| CTGCTCTGCAGAAATCTCTA+AGG | - | chr3.2:72000670-72000689 | MS.gene77471:intron | 45.0% | |
| CTTAGAGATTTCTGCAGAGC+AGG | + | chr3.2:72000672-72000691 | None:intergenic | 45.0% | |
| CTTCGATGGCTATATGAGCA+AGG | - | chr3.2:72001475-72001494 | MS.gene77471:CDS | 45.0% | |
| GAAACTTAGAGCCTTCTGCA+AGG | - | chr3.2:72001183-72001202 | MS.gene77471:CDS | 45.0% | |
| GTCCTTGAAGCCATCAAACA+AGG | - | chr3.2:72000489-72000508 | MS.gene77471:CDS | 45.0% | |
| GTTACTAGCTCCAATGGCTT+TGG | + | chr3.2:72000950-72000969 | None:intergenic | 45.0% | |
| GTTTCTCTTGTTGCCATCCA+AGG | + | chr3.2:72001168-72001187 | None:intergenic | 45.0% | |
| TTTGATGCAGCTGCTGCATA+TGG | - | chr3.2:72000522-72000541 | MS.gene77471:CDS | 45.0% | |
| ! | GAAAGGTGTGTGGACATCAT+TGG | - | chr3.2:72000900-72000919 | MS.gene77471:intron | 45.0% |
| ! | GTAACCTTACTGGTTGCTTC+CGG | + | chr3.2:72000456-72000475 | None:intergenic | 45.0% |
| ! | TCATCAAGAAGAGGCTTGGT+TGG | + | chr3.2:72001631-72001650 | None:intergenic | 45.0% |
| !! | CAGGGGTGCTAATTTAGTGA+TGG | - | chr3.2:72001255-72001274 | MS.gene77471:intron | 45.0% |
| !!! | AATTATTGATTAAATTAATT+TGG | - | chr3.2:72001121-72001140 | MS.gene77471:CDS | 5.0% |
| ACAACGGAAAATGCCGGTGA+TGG | - | chr3.2:72000416-72000435 | MS.gene77471:CDS | 50.0% | |
| ACCTTTCCTAAGGGGTGAGA+AGG | + | chr3.2:72001230-72001249 | None:intergenic | 50.0% | |
| AGAGCAGGAACAATGAGCTC+AGG | + | chr3.2:72000657-72000676 | None:intergenic | 50.0% | |
| AGGACTACTTCAGGGACTGT+AGG | + | chr3.2:72000381-72000400 | None:intergenic | 50.0% | |
| AGGTGGAAGTCAACCTTGGA+TGG | - | chr3.2:72001152-72001171 | MS.gene77471:CDS | 50.0% | |
| CAACGGAAAATGCCGGTGAT+GGG | - | chr3.2:72000417-72000436 | MS.gene77471:CDS | 50.0% | |
| CCTGCTAGCACCTTTCCTAA+GGG | + | chr3.2:72001239-72001258 | None:intergenic | 50.0% | |
| CTGCTAGCACCTTTCCTAAG+GGG | + | chr3.2:72001238-72001257 | None:intergenic | 50.0% | |
| CTTGGTCTCACCAAAGCCAT+TGG | - | chr3.2:72000937-72000956 | MS.gene77471:intron | 50.0% | |
| TAACTGCCTTCTCACCCCTT+AGG | - | chr3.2:72001221-72001240 | MS.gene77471:CDS | 50.0% | |
| TTGATTCACTGCAGGAGGGA+TGG | + | chr3.2:72001013-72001032 | None:intergenic | 50.0% | |
| !! | CCTTAGGAAAGGTGCTAGCA+GGG | - | chr3.2:72001237-72001256 | MS.gene77471:intron | 50.0% |
| !! | CTTAGGAAAGGTGCTAGCAG+GGG | - | chr3.2:72001238-72001257 | MS.gene77471:intron | 50.0% |
| AGGAGGGGAGGACTACTTCA+GGG | + | chr3.2:72000389-72000408 | None:intergenic | 55.0% | |
| CACCGGACAACGGAAAATGC+CGG | - | chr3.2:72000410-72000429 | MS.gene77471:CDS | 55.0% | |
| CCCTGCTAGCACCTTTCCTA+AGG | + | chr3.2:72001240-72001259 | None:intergenic | 55.0% | |
| GCCTTCTCACCCCTTAGGAA+AGG | - | chr3.2:72001226-72001245 | MS.gene77471:CDS | 55.0% | |
| TGCAGCTGCTGCATATGGTG+TGG | - | chr3.2:72000527-72000546 | MS.gene77471:CDS | 55.0% | |
| TGCAGGAGGGATGGTTGCAA+AGG | + | chr3.2:72001004-72001023 | None:intergenic | 55.0% | |
| ! | CACCGGCATTTTCCGTTGTC+CGG | + | chr3.2:72000415-72000434 | None:intergenic | 55.0% |
| ! | CATTTTCCGTTGTCCGGTGG+AGG | + | chr3.2:72000409-72000428 | None:intergenic | 55.0% |
| ! | CTTACTGGTTGCTTCCGGTG+CGG | + | chr3.2:72000451-72000470 | None:intergenic | 55.0% |
| !! | AAAATGCCGGTGATGGGCCT+CGG | - | chr3.2:72000423-72000442 | MS.gene77471:CDS | 55.0% |
| !! | CCCTTAGGAAAGGTGCTAGC+AGG | - | chr3.2:72001236-72001255 | MS.gene77471:intron | 55.0% |
| CGCACCGGAAGCAACCAGTA+AGG | - | chr3.2:72000449-72000468 | MS.gene77471:CDS | 60.0% | |
| GAGGAGGGGAGGACTACTTC+AGG | + | chr3.2:72000390-72000409 | None:intergenic | 60.0% | |
| TTCCGTTGTCCGGTGGAGGA+GGG | + | chr3.2:72000405-72000424 | None:intergenic | 60.0% | |
| TTTCCGTTGTCCGGTGGAGG+AGG | + | chr3.2:72000406-72000425 | None:intergenic | 60.0% | |
| ! | CGGCATTTTCCGTTGTCCGG+TGG | + | chr3.2:72000412-72000431 | None:intergenic | 60.0% |
| GTAGTCCTCCCCTCCTCCAC+CGG | - | chr3.2:72000393-72000412 | MS.gene77471:CDS | 65.0% | |
| TCCCCTCCTCCACCGGACAA+CGG | - | chr3.2:72000400-72000419 | MS.gene77471:CDS | 65.0% | |
| TCCGTTGTCCGGTGGAGGAG+GGG | + | chr3.2:72000404-72000423 | None:intergenic | 65.0% | |
| GTTGTCCGGTGGAGGAGGGG+AGG | + | chr3.2:72000401-72000420 | None:intergenic | 70.0% | |
| ! | TTGCTTCCGGTGCGGTGCCG+AGG | + | chr3.2:72000443-72000462 | None:intergenic | 70.0% |
| GATGGGCCTCGGCACCGCAC+CGG | - | chr3.2:72000434-72000453 | MS.gene77471:CDS | 75.0% | |
| ! | GCGGTGCCGAGGCCCATCAC+CGG | + | chr3.2:72000432-72000451 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 72000366 | 72001669 | 72000366 | ID=MS.gene77471 |
| chr3.2 | mRNA | 72000366 | 72001669 | 72000366 | ID=MS.gene77471.t1;Parent=MS.gene77471 |
| chr3.2 | exon | 72001344 | 72001669 | 72001344 | ID=MS.gene77471.t1.exon1;Parent=MS.gene77471.t1 |
| chr3.2 | CDS | 72001344 | 72001669 | 72001344 | ID=cds.MS.gene77471.t1;Parent=MS.gene77471.t1 |
| chr3.2 | exon | 72001003 | 72001246 | 72001003 | ID=MS.gene77471.t1.exon2;Parent=MS.gene77471.t1 |
| chr3.2 | CDS | 72001003 | 72001246 | 72001003 | ID=cds.MS.gene77471.t1;Parent=MS.gene77471.t1 |
| chr3.2 | exon | 72000705 | 72000881 | 72000705 | ID=MS.gene77471.t1.exon3;Parent=MS.gene77471.t1 |
| chr3.2 | CDS | 72000705 | 72000881 | 72000705 | ID=cds.MS.gene77471.t1;Parent=MS.gene77471.t1 |
| chr3.2 | exon | 72000366 | 72000566 | 72000366 | ID=MS.gene77471.t1.exon4;Parent=MS.gene77471.t1 |
| chr3.2 | CDS | 72000366 | 72000566 | 72000366 | ID=cds.MS.gene77471.t1;Parent=MS.gene77471.t1 |
| Gene Sequence |
| Protein sequence |
