Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70420.t1 | XP_003601570.1 | 99.4 | 315 | 2 | 0 | 1 | 315 | 1 | 315 | 2.40E-174 | 621.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70420.t1 | P26690 | 69.2 | 315 | 96 | 1 | 1 | 315 | 1 | 314 | 3.2e-130 | 466.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70420.t1 | G7J9I2 | 99.4 | 315 | 2 | 0 | 1 | 315 | 1 | 315 | 1.8e-174 | 621.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050216 | MS.gene70420 | -0.804671 | 1.93E-49 | -1.69E-46 |
| MS.gene050380 | MS.gene70420 | 0.869503 | 2.95E-66 | -1.69E-46 |
| MS.gene050381 | MS.gene70420 | 0.883271 | 5.17E-71 | -1.69E-46 |
| MS.gene050499 | MS.gene70420 | -0.801674 | 8.05E-49 | -1.69E-46 |
| MS.gene050502 | MS.gene70420 | -0.817411 | 3.34E-52 | -1.69E-46 |
| MS.gene050868 | MS.gene70420 | -0.811681 | 6.20E-51 | -1.69E-46 |
| MS.gene050871 | MS.gene70420 | -0.816809 | 4.57E-52 | -1.69E-46 |
| MS.gene050883 | MS.gene70420 | 0.819296 | 1.25E-52 | -1.69E-46 |
| MS.gene051127 | MS.gene70420 | 0.825773 | 3.90E-54 | -1.69E-46 |
| MS.gene05141 | MS.gene70420 | 0.805826 | 1.11E-49 | -1.69E-46 |
| MS.gene051509 | MS.gene70420 | -0.8082 | 3.48E-50 | -1.69E-46 |
| MS.gene051946 | MS.gene70420 | 0.807301 | 5.40E-50 | -1.69E-46 |
| MS.gene053319 | MS.gene70420 | 0.841778 | 3.87E-58 | -1.69E-46 |
| MS.gene053406 | MS.gene70420 | 0.810102 | 1.36E-50 | -1.69E-46 |
| MS.gene053420 | MS.gene70420 | 0.804704 | 1.90E-49 | -1.69E-46 |
| MS.gene053574 | MS.gene70420 | -0.806166 | 9.39E-50 | -1.69E-46 |
| MS.gene053626 | MS.gene70420 | -0.803905 | 2.79E-49 | -1.69E-46 |
| MS.gene054003 | MS.gene70420 | -0.800089 | 1.70E-48 | -1.69E-46 |
| MS.gene054595 | MS.gene70420 | -0.815702 | 8.07E-52 | -1.69E-46 |
| MS.gene055124 | MS.gene70420 | 0.858714 | 6.82E-63 | -1.69E-46 |
| MS.gene055139 | MS.gene70420 | 0.806079 | 9.79E-50 | -1.69E-46 |
| MS.gene055154 | MS.gene70420 | 0.824267 | 8.86E-54 | -1.69E-46 |
| MS.gene055401 | MS.gene70420 | 0.838261 | 3.20E-57 | -1.69E-46 |
| MS.gene055959 | MS.gene70420 | 0.810086 | 1.37E-50 | -1.69E-46 |
| MS.gene056166 | MS.gene70420 | -0.822777 | 1.98E-53 | -1.69E-46 |
| MS.gene056310 | MS.gene70420 | 0.873511 | 1.39E-67 | -1.69E-46 |
| MS.gene056311 | MS.gene70420 | 0.829847 | 4.09E-55 | -1.69E-46 |
| MS.gene056313 | MS.gene70420 | 0.900628 | 6.06E-78 | -1.69E-46 |
| MS.gene056408 | MS.gene70420 | 0.806253 | 9.00E-50 | -1.69E-46 |
| MS.gene056422 | MS.gene70420 | -0.839185 | 1.84E-57 | -1.69E-46 |
| MS.gene057086 | MS.gene70420 | -0.812997 | 3.20E-51 | -1.69E-46 |
| MS.gene057506 | MS.gene70420 | 0.812603 | 3.90E-51 | -1.69E-46 |
| MS.gene058099 | MS.gene70420 | 0.816745 | 4.72E-52 | -1.69E-46 |
| MS.gene058108 | MS.gene70420 | 0.854888 | 9.12E-62 | -1.69E-46 |
| MS.gene058109 | MS.gene70420 | 0.839469 | 1.56E-57 | -1.69E-46 |
| MS.gene058170 | MS.gene70420 | 0.837118 | 6.27E-57 | -1.69E-46 |
| MS.gene058171 | MS.gene70420 | 0.861478 | 9.98E-64 | -1.69E-46 |
| MS.gene058172 | MS.gene70420 | 0.806978 | 6.33E-50 | -1.69E-46 |
| MS.gene058173 | MS.gene70420 | 0.858895 | 6.02E-63 | -1.69E-46 |
| MS.gene058174 | MS.gene70420 | 0.83933 | 1.69E-57 | -1.69E-46 |
| MS.gene058175 | MS.gene70420 | 0.862279 | 5.67E-64 | -1.69E-46 |
| MS.gene058176 | MS.gene70420 | 0.844207 | 8.72E-59 | -1.69E-46 |
| MS.gene058179 | MS.gene70420 | 0.864851 | 9.03E-65 | -1.69E-46 |
| MS.gene058180 | MS.gene70420 | 0.870882 | 1.04E-66 | -1.69E-46 |
| MS.gene05837 | MS.gene70420 | -0.813438 | 2.56E-51 | -1.69E-46 |
| MS.gene058420 | MS.gene70420 | -0.80743 | 5.07E-50 | -1.69E-46 |
| MS.gene058840 | MS.gene70420 | 0.824432 | 8.10E-54 | -1.69E-46 |
| MS.gene059037 | MS.gene70420 | 0.860744 | 1.67E-63 | -1.69E-46 |
| MS.gene059038 | MS.gene70420 | 0.809353 | 1.97E-50 | -1.69E-46 |
| MS.gene059044 | MS.gene70420 | 0.838753 | 2.38E-57 | -1.69E-46 |
| MS.gene059045 | MS.gene70420 | 0.839712 | 1.34E-57 | -1.69E-46 |
| MS.gene059223 | MS.gene70420 | 0.802646 | 5.08E-49 | -1.69E-46 |
| MS.gene059367 | MS.gene70420 | -0.829872 | 4.04E-55 | -1.69E-46 |
| MS.gene059368 | MS.gene70420 | -0.825077 | 5.70E-54 | -1.69E-46 |
| MS.gene059517 | MS.gene70420 | -0.836737 | 7.85E-57 | -1.69E-46 |
| MS.gene059680 | MS.gene70420 | -0.815424 | 9.31E-52 | -1.69E-46 |
| MS.gene059694 | MS.gene70420 | 0.801607 | 8.31E-49 | -1.69E-46 |
| MS.gene059697 | MS.gene70420 | 0.820447 | 6.83E-53 | -1.69E-46 |
| MS.gene059944 | MS.gene70420 | 0.837061 | 6.49E-57 | -1.69E-46 |
| MS.gene060482 | MS.gene70420 | -0.845166 | 4.81E-59 | -1.69E-46 |
| MS.gene060808 | MS.gene70420 | 0.814888 | 1.23E-51 | -1.69E-46 |
| MS.gene060837 | MS.gene70420 | -0.806209 | 9.19E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene70420 | MS.gene91446 | PPI |
| MS.gene003638 | MS.gene70420 | PPI |
| MS.gene70420 | MS.gene008794 | PPI |
| MS.gene62620 | MS.gene70420 | PPI |
| MS.gene056488 | MS.gene70420 | PPI |
| MS.gene70420 | MS.gene98697 | PPI |
| MS.gene70420 | MS.gene93783 | PPI |
| MS.gene70420 | MS.gene93058 | PPI |
| MS.gene93059 | MS.gene70420 | PPI |
| MS.gene70420 | MS.gene99693 | PPI |
| MS.gene72560 | MS.gene70420 | PPI |
| MS.gene70420 | MS.gene42146 | PPI |
| MS.gene040151 | MS.gene70420 | PPI |
| MS.gene97981 | MS.gene70420 | PPI |
| MS.gene062932 | MS.gene70420 | PPI |
| MS.gene70420 | MS.gene97980 | PPI |
| MS.gene54933 | MS.gene70420 | PPI |
| MS.gene95889 | MS.gene70420 | PPI |
| MS.gene056487 | MS.gene70420 | PPI |
| MS.gene70420 | MS.gene008793 | PPI |
| MS.gene50700 | MS.gene70420 | PPI |
| MS.gene55382 | MS.gene70420 | PPI |
| MS.gene70420 | MS.gene87111 | PPI |
| MS.gene70420 | MS.gene024362 | PPI |
| MS.gene42873 | MS.gene70420 | PPI |
| MS.gene70420 | MS.gene53169 | PPI |
| MS.gene23359 | MS.gene70420 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70420.t1 | MTR_3g083130 | 99.365 | 315 | 2 | 0 | 1 | 315 | 1 | 315 | 0.0 | 640 |
| MS.gene70420.t1 | MTR_5g097900 | 68.831 | 308 | 95 | 1 | 7 | 314 | 6 | 312 | 2.56e-155 | 437 |
| MS.gene70420.t1 | MTR_5g097910 | 68.506 | 308 | 96 | 1 | 7 | 314 | 6 | 312 | 6.28e-155 | 436 |
| MS.gene70420.t1 | MTR_5g097940 | 68.506 | 308 | 96 | 1 | 7 | 314 | 6 | 312 | 6.28e-155 | 436 |
| MS.gene70420.t1 | MTR_0374s0050 | 63.175 | 315 | 113 | 3 | 3 | 315 | 2 | 315 | 4.07e-144 | 409 |
| MS.gene70420.t1 | MTR_4g072320 | 50.949 | 316 | 137 | 5 | 7 | 313 | 6 | 312 | 1.45e-107 | 316 |
| MS.gene70420.t1 | MTR_6g073110 | 51.656 | 302 | 135 | 4 | 6 | 302 | 5 | 300 | 7.25e-105 | 309 |
| MS.gene70420.t1 | MTR_4g072350 | 50.000 | 318 | 143 | 6 | 7 | 313 | 6 | 318 | 1.17e-104 | 309 |
| MS.gene70420.t1 | MTR_4g072360 | 49.221 | 321 | 147 | 6 | 4 | 313 | 3 | 318 | 8.06e-104 | 306 |
| MS.gene70420.t1 | MTR_4g036845 | 40.328 | 305 | 168 | 7 | 21 | 315 | 14 | 314 | 5.28e-75 | 233 |
| MS.gene70420.t1 | MTR_8g070095 | 41.053 | 285 | 164 | 3 | 20 | 302 | 15 | 297 | 5.86e-72 | 225 |
| MS.gene70420.t1 | MTR_4g021350 | 45.583 | 283 | 148 | 5 | 20 | 302 | 15 | 291 | 1.37e-70 | 221 |
| MS.gene70420.t1 | MTR_8g070115 | 40.984 | 305 | 166 | 5 | 20 | 315 | 15 | 314 | 7.42e-69 | 217 |
| MS.gene70420.t1 | MTR_7g063580 | 41.639 | 305 | 163 | 5 | 20 | 315 | 55 | 353 | 2.24e-68 | 217 |
| MS.gene70420.t1 | MTR_4g021350 | 45.126 | 277 | 146 | 5 | 20 | 296 | 15 | 285 | 9.99e-68 | 213 |
| MS.gene70420.t1 | MTR_4g021410 | 41.447 | 304 | 155 | 5 | 20 | 315 | 16 | 304 | 7.69e-66 | 209 |
| MS.gene70420.t1 | MTR_7g070500 | 37.459 | 307 | 171 | 7 | 21 | 315 | 23 | 320 | 7.82e-64 | 204 |
| MS.gene70420.t1 | MTR_4g021450 | 40.789 | 304 | 157 | 5 | 20 | 315 | 16 | 304 | 1.19e-62 | 201 |
| MS.gene70420.t1 | MTR_4g021410 | 41.818 | 275 | 139 | 4 | 49 | 315 | 6 | 267 | 1.17e-61 | 197 |
| MS.gene70420.t1 | MTR_7g070500 | 38.909 | 275 | 159 | 5 | 21 | 294 | 23 | 289 | 1.60e-61 | 198 |
| MS.gene70420.t1 | MTR_4g072060 | 37.500 | 304 | 174 | 6 | 20 | 312 | 10 | 308 | 9.68e-54 | 178 |
| MS.gene70420.t1 | MTR_4g021410 | 41.667 | 240 | 120 | 3 | 84 | 315 | 1 | 228 | 1.91e-50 | 167 |
| MS.gene70420.t1 | MTR_4g021450 | 41.250 | 240 | 121 | 3 | 84 | 315 | 1 | 228 | 2.51e-48 | 161 |
| MS.gene70420.t1 | MTR_4g021450 | 39.674 | 184 | 100 | 2 | 140 | 315 | 5 | 185 | 1.48e-40 | 140 |
| MS.gene70420.t1 | MTR_6g072650 | 46.000 | 150 | 45 | 5 | 175 | 313 | 10 | 134 | 3.49e-30 | 111 |
| MS.gene70420.t1 | MTR_6g072710 | 48.673 | 113 | 53 | 2 | 193 | 302 | 1 | 111 | 1.23e-27 | 104 |
| MS.gene70420.t1 | MTR_7g114990 | 25.850 | 294 | 161 | 9 | 44 | 302 | 54 | 325 | 1.24e-13 | 70.9 |
| MS.gene70420.t1 | MTR_3g449790 | 25.325 | 308 | 161 | 10 | 39 | 308 | 77 | 353 | 2.86e-13 | 70.1 |
| MS.gene70420.t1 | MTR_3g092140 | 23.103 | 290 | 169 | 10 | 45 | 297 | 79 | 351 | 5.78e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70420.t1 | AT1G59960 | 51.692 | 325 | 138 | 7 | 6 | 315 | 5 | 325 | 1.51e-114 | 334 |
| MS.gene70420.t1 | AT1G59950 | 52.997 | 317 | 137 | 5 | 10 | 315 | 4 | 319 | 3.14e-110 | 323 |
| MS.gene70420.t1 | AT5G01670 | 40.645 | 310 | 159 | 9 | 20 | 315 | 23 | 321 | 2.92e-73 | 229 |
| MS.gene70420.t1 | AT2G37790 | 42.434 | 304 | 162 | 6 | 20 | 315 | 15 | 313 | 3.50e-73 | 228 |
| MS.gene70420.t1 | AT2G37770 | 41.830 | 306 | 162 | 6 | 20 | 315 | 15 | 314 | 9.55e-73 | 227 |
| MS.gene70420.t1 | AT5G62420 | 39.869 | 306 | 169 | 7 | 21 | 315 | 14 | 315 | 3.93e-71 | 223 |
| MS.gene70420.t1 | AT2G37760 | 39.344 | 305 | 167 | 5 | 20 | 315 | 15 | 310 | 9.85e-68 | 214 |
| MS.gene70420.t1 | AT2G37760 | 39.344 | 305 | 167 | 5 | 20 | 315 | 15 | 310 | 9.85e-68 | 214 |
| MS.gene70420.t1 | AT2G37760 | 41.429 | 280 | 154 | 4 | 20 | 298 | 15 | 285 | 2.44e-67 | 213 |
| MS.gene70420.t1 | AT5G01670 | 37.389 | 337 | 159 | 10 | 20 | 315 | 23 | 348 | 2.76e-67 | 214 |
| MS.gene70420.t1 | AT2G37760 | 40.780 | 282 | 157 | 4 | 20 | 300 | 15 | 287 | 4.07e-67 | 212 |
| MS.gene70420.t1 | AT2G37760 | 40.780 | 282 | 157 | 4 | 20 | 300 | 15 | 287 | 4.07e-67 | 212 |
| MS.gene70420.t1 | AT3G53880 | 38.235 | 306 | 173 | 6 | 20 | 315 | 15 | 314 | 1.88e-63 | 203 |
| MS.gene70420.t1 | AT3G53880 | 38.489 | 278 | 165 | 4 | 20 | 296 | 15 | 287 | 1.63e-59 | 193 |
| MS.gene70420.t1 | AT2G21250 | 34.868 | 304 | 182 | 6 | 20 | 312 | 10 | 308 | 5.14e-59 | 192 |
| MS.gene70420.t1 | AT2G21260 | 35.764 | 288 | 169 | 6 | 20 | 296 | 10 | 292 | 2.50e-58 | 190 |
| MS.gene70420.t1 | AT2G37760 | 38.550 | 262 | 149 | 4 | 63 | 315 | 6 | 264 | 3.17e-55 | 181 |
| MS.gene70420.t1 | AT2G37760 | 38.550 | 262 | 149 | 4 | 63 | 315 | 6 | 264 | 3.17e-55 | 181 |
| MS.gene70420.t1 | AT2G37770 | 46.354 | 192 | 98 | 3 | 20 | 211 | 15 | 201 | 1.31e-49 | 167 |
| MS.gene70420.t1 | AT2G21250 | 37.118 | 229 | 128 | 6 | 20 | 237 | 10 | 233 | 1.73e-44 | 152 |
| MS.gene70420.t1 | AT2G21260 | 32.258 | 217 | 134 | 4 | 89 | 294 | 8 | 222 | 1.58e-36 | 131 |
| MS.gene70420.t1 | AT1G06690 | 26.102 | 295 | 165 | 7 | 39 | 297 | 88 | 365 | 3.19e-18 | 84.7 |
| MS.gene70420.t1 | AT5G53580 | 25.623 | 281 | 153 | 12 | 45 | 287 | 80 | 342 | 5.88e-13 | 69.3 |
| MS.gene70420.t1 | AT1G60680 | 24.573 | 293 | 167 | 12 | 45 | 302 | 49 | 322 | 6.91e-11 | 62.8 |
| MS.gene70420.t1 | AT1G60750 | 27.451 | 306 | 174 | 13 | 21 | 302 | 25 | 306 | 8.79e-11 | 62.4 |
Find 84 sgRNAs with CRISPR-Local
Find 103 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTTGTTACTTCTAAATTA+TGG | 0.129563 | 3.1:+66087547 | MS.gene70420:CDS |
| AGCCTCTTCTTGATGATCTA+TGG | 0.232119 | 3.1:+66088566 | MS.gene70420:CDS |
| CTTTGATGAGTCTCTCTTGA+TGG | 0.239320 | 3.1:-66088534 | None:intergenic |
| AATCAAAGATTTGCAAGTTT+TGG | 0.245163 | 3.1:-66088471 | None:intergenic |
| TCACCTGGTTTAGTAGTAAT+TGG | 0.253992 | 3.1:-66087763 | None:intergenic |
| CTTTGTTACTTCTAAATTAT+GGG | 0.275213 | 3.1:+66087548 | MS.gene70420:CDS |
| ATGTCTATAACCTTGTTTGA+TGG | 0.330198 | 3.1:-66087430 | None:intergenic |
| ACAATCTCTGATACTTCAAT+AGG | 0.347637 | 3.1:-66087796 | None:intergenic |
| AAAACTTGCAAATCTTTGAT+TGG | 0.362637 | 3.1:+66088473 | MS.gene70420:CDS |
| AACCTTGTTTGATGGCTTCA+AGG | 0.381003 | 3.1:-66087422 | None:intergenic |
| CCCTGCTAGCACCTTTCCTA+AGG | 0.387480 | 3.1:-66088168 | None:intergenic |
| CAATGATATACTCAAAGAAT+TGG | 0.390203 | 3.1:+66088210 | MS.gene70420:CDS |
| GTAACCTTACTGGTTGCTTC+CGG | 0.403522 | 3.1:-66087384 | None:intergenic |
| TAGAGCCTTCTGCAAGGAAA+AGG | 0.424089 | 3.1:+66088120 | MS.gene70420:CDS |
| TTTCCGTTGTCCGGTGGAGG+AGG | 0.424568 | 3.1:-66087334 | None:intergenic |
| TTGGCCAATGGATCAAAATA+AGG | 0.425035 | 3.1:-66087744 | None:intergenic |
| CTTAGAGATTTCTGCAGAGC+AGG | 0.429110 | 3.1:-66087600 | None:intergenic |
| CTGCTCTGCAGAAATCTCTA+AGG | 0.431594 | 3.1:+66087601 | MS.gene70420:CDS |
| GTTGCTAGCTCCAATGGCTT+TGG | 0.432705 | 3.1:-66087878 | None:intergenic |
| CCCTTAGGAAAGGTGCTAGC+AGG | 0.441076 | 3.1:+66088167 | MS.gene70420:CDS |
| GAGGAGGGGAGGACTACTTC+AGG | 0.445285 | 3.1:-66087318 | None:intergenic |
| CAATCTCTGATACTTCAATA+GGG | 0.455333 | 3.1:-66087795 | None:intergenic |
| GTTTCTCTTGTTGCCATCCA+AGG | 0.461119 | 3.1:-66088096 | None:intergenic |
| TTCCGTTGTCCGGTGGAGGA+GGG | 0.463761 | 3.1:-66087333 | None:intergenic |
| GACTCTTCAACTAGAATACT+TGG | 0.468397 | 3.1:+66087720 | MS.gene70420:CDS |
| AAAATGCCGGTGATGGGCCT+CGG | 0.468907 | 3.1:+66087354 | MS.gene70420:CDS |
| TTTGATGCAGCTGCTGCATA+TGG | 0.474976 | 3.1:+66087453 | MS.gene70420:CDS |
| TCCGTTGTCCGGTGGAGGAG+GGG | 0.476698 | 3.1:-66087332 | None:intergenic |
| GCCTCTTCTTGATGATCTAT+GGG | 0.483427 | 3.1:+66088567 | MS.gene70420:CDS |
| CCTGCTAGCACCTTTCCTAA+GGG | 0.491638 | 3.1:-66088167 | None:intergenic |
| TGAGAAGTTGCTAGCTCCAA+TGG | 0.493072 | 3.1:-66087884 | None:intergenic |
| ACAACGGAAAATGCCGGTGA+TGG | 0.497720 | 3.1:+66087347 | MS.gene70420:CDS |
| CTTGGTCTCACCAAAGCCAT+TGG | 0.503945 | 3.1:+66087868 | MS.gene70420:CDS |
| TCAATTACTTGATTCACTGC+AGG | 0.507920 | 3.1:-66087949 | None:intergenic |
| TTTGGCAGATTTGTCTTCGA+TGG | 0.511955 | 3.1:+66088392 | MS.gene70420:intron |
| GAAAGGTGTGTGGACATCAT+TGG | 0.513831 | 3.1:+66087831 | MS.gene70420:CDS |
| ATTGCAGAAGCACTTAAACT+TGG | 0.516828 | 3.1:+66087501 | MS.gene70420:CDS |
| CAACGGAAAATGCCGGTGAT+GGG | 0.522929 | 3.1:+66087348 | MS.gene70420:CDS |
| TAACTGCCTTCTCACCCCTT+AGG | 0.527768 | 3.1:+66088152 | MS.gene70420:CDS |
| CCTTAGGAAAGGTGCTAGCA+GGG | 0.529822 | 3.1:+66088168 | MS.gene70420:CDS |
| ATAGGGTATTTAACTTCACC+TGG | 0.535676 | 3.1:-66087778 | None:intergenic |
| TTGCTTCCGGTGCGGTGCCG+AGG | 0.538965 | 3.1:-66087371 | None:intergenic |
| GTAGTCCTCCCCTCCTCCAC+CGG | 0.540364 | 3.1:+66087324 | MS.gene70420:CDS |
| TTGATTCACTGCAGGAGGGA+TGG | 0.540989 | 3.1:-66087941 | None:intergenic |
| ACCTTTCCTAAGGGGTGAGA+AGG | 0.541355 | 3.1:-66088158 | None:intergenic |
| AGAGCCTTCTGCAAGGAAAA+GGG | 0.543832 | 3.1:+66088121 | MS.gene70420:CDS |
| GTTGTCCGGTGGAGGAGGGG+AGG | 0.547684 | 3.1:-66087329 | None:intergenic |
| AGGTGGAAGTCAACCTTGGA+TGG | 0.547815 | 3.1:+66088083 | MS.gene70420:intron |
| GATGGGCCTCGGCACCGCAC+CGG | 0.552144 | 3.1:+66087365 | MS.gene70420:CDS |
| TATTGAAGTATCAGAGATTG+TGG | 0.554195 | 3.1:+66087798 | MS.gene70420:CDS |
| AGGACTACTTCAGGGACTGT+AGG | 0.556376 | 3.1:-66087309 | None:intergenic |
| TAGATCATCAAGAAGAGGCT+TGG | 0.556426 | 3.1:-66088563 | None:intergenic |
| TCATCAAGAAGAGGCTTGGT+TGG | 0.556818 | 3.1:-66088559 | None:intergenic |
| AGAGCAGGAACAATGAGCTC+AGG | 0.564156 | 3.1:-66087585 | None:intergenic |
| ATTACTTGATTCACTGCAGG+AGG | 0.566545 | 3.1:-66087946 | None:intergenic |
| TCCCCTCCTCCACCGGACAA+CGG | 0.566967 | 3.1:+66087331 | MS.gene70420:CDS |
| GCGGTGCCGAGGCCCATCAC+CGG | 0.568831 | 3.1:-66087360 | None:intergenic |
| TAATAGGTGGAAGTCAACCT+TGG | 0.576953 | 3.1:+66088079 | MS.gene70420:intron |
| GTTTAGTAGTAATTGGCCAA+TGG | 0.578679 | 3.1:-66087756 | None:intergenic |
| TGCAGGAGGGATGGTTGCAA+AGG | 0.578968 | 3.1:-66087932 | None:intergenic |
| TCCCATAGATCATCAAGAAG+AGG | 0.583002 | 3.1:-66088568 | None:intergenic |
| CGCACCGGAAGCAACCAGTA+AGG | 0.584380 | 3.1:+66087380 | MS.gene70420:CDS |
| ACATGGCAAGACTATAGCTC+AGG | 0.585566 | 3.1:+66088240 | MS.gene70420:CDS |
| CTTCGATGGCTATATGAGCA+AGG | 0.590577 | 3.1:+66088406 | MS.gene70420:CDS |
| GTCCTTGAAGCCATCAAACA+AGG | 0.591634 | 3.1:+66087420 | MS.gene70420:CDS |
| CACCGGACAACGGAAAATGC+CGG | 0.597803 | 3.1:+66087341 | MS.gene70420:CDS |
| CAGGGGTGCTAATTTAGTGA+TGG | 0.600153 | 3.1:+66088186 | MS.gene70420:CDS |
| AGGAGGGGAGGACTACTTCA+GGG | 0.602610 | 3.1:-66087317 | None:intergenic |
| TTAAGAGCTATGACAAGGAG+AGG | 0.604664 | 3.1:+66088443 | MS.gene70420:CDS |
| GCCTTCTCACCCCTTAGGAA+AGG | 0.609336 | 3.1:+66088157 | MS.gene70420:CDS |
| AAAGAATTGGCTGATGCACA+TGG | 0.613616 | 3.1:+66088223 | MS.gene70420:CDS |
| CTTTGATTGGTCATTGACTG+AGG | 0.619859 | 3.1:+66088486 | MS.gene70420:CDS |
| CTTACTGGTTGCTTCCGGTG+CGG | 0.628936 | 3.1:-66087379 | None:intergenic |
| CTGCAAGGAAAAGGGTATTG+TGG | 0.631813 | 3.1:+66088129 | MS.gene70420:CDS |
| CTGCTAGCACCTTTCCTAAG+GGG | 0.635830 | 3.1:-66088166 | None:intergenic |
| TGTGGTTAAGAGCTATGACA+AGG | 0.641038 | 3.1:+66088438 | MS.gene70420:CDS |
| CATCAAGAGAGACTCATCAA+AGG | 0.641861 | 3.1:+66088535 | MS.gene70420:CDS |
| TGGCCAATTACTACTAAACC+AGG | 0.647399 | 3.1:+66087760 | MS.gene70420:CDS |
| ATTGTGGAATTTGATATGAA+AGG | 0.649138 | 3.1:+66087814 | MS.gene70420:CDS |
| GAAACTTAGAGCCTTCTGCA+AGG | 0.650710 | 3.1:+66088114 | MS.gene70420:CDS |
| CTTAGGAAAGGTGCTAGCAG+GGG | 0.654145 | 3.1:+66088169 | MS.gene70420:CDS |
| TTACTTGATTCACTGCAGGA+GGG | 0.664815 | 3.1:-66087945 | None:intergenic |
| AATTTGATATGAAAGGTGTG+TGG | 0.691006 | 3.1:+66087821 | MS.gene70420:CDS |
| TGCAGCTGCTGCATATGGTG+TGG | 0.691514 | 3.1:+66087458 | MS.gene70420:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTAATGTCACAATACTT+TGG | + | chr3.1:66088374-66088393 | MS.gene70420:intron | 20.0% |
| !! | ACATATGAAATCATGTAAAA+TGG | - | chr3.1:66088009-66088028 | None:intergenic | 20.0% |
| !! | TATATAATATGATCTTAGCA+TGG | + | chr3.1:66088272-66088291 | MS.gene70420:intron | 20.0% |
| !!! | AAATTAATTTGGATCCTAAT+AGG | + | chr3.1:66088063-66088082 | MS.gene70420:intron | 20.0% |
| !!! | CTTTGTTACTTCTAAATTAT+GGG | + | chr3.1:66087548-66087567 | MS.gene70420:CDS | 20.0% |
| !!! | TCTTTGTTACTTCTAAATTA+TGG | + | chr3.1:66087547-66087566 | MS.gene70420:CDS | 20.0% |
| ! | AAAACTTGCAAATCTTTGAT+TGG | + | chr3.1:66088473-66088492 | MS.gene70420:CDS | 25.0% |
| ! | ATTGTGGAATTTGATATGAA+AGG | + | chr3.1:66087814-66087833 | MS.gene70420:CDS | 25.0% |
| ! | CAATGATATACTCAAAGAAT+TGG | + | chr3.1:66088210-66088229 | MS.gene70420:CDS | 25.0% |
| !! | AATCAAAGATTTGCAAGTTT+TGG | - | chr3.1:66088474-66088493 | None:intergenic | 25.0% |
| AATCACTCCAAATTAGAAGT+TGG | - | chr3.1:66088299-66088318 | None:intergenic | 30.0% | |
| ACAATCTCTGATACTTCAAT+AGG | - | chr3.1:66087799-66087818 | None:intergenic | 30.0% | |
| CAATCTCTGATACTTCAATA+GGG | - | chr3.1:66087798-66087817 | None:intergenic | 30.0% | |
| TTGGAAGAATGTCAAAAACT+TGG | + | chr3.1:66087850-66087869 | MS.gene70420:CDS | 30.0% | |
| ! | AATTTGATATGAAAGGTGTG+TGG | + | chr3.1:66087821-66087840 | MS.gene70420:CDS | 30.0% |
| ! | ATCTTTTGTAGTAACCTTAC+TGG | - | chr3.1:66087397-66087416 | None:intergenic | 30.0% |
| ! | ATGTCTATAACCTTGTTTGA+TGG | - | chr3.1:66087433-66087452 | None:intergenic | 30.0% |
| ! | TATTGAAGTATCAGAGATTG+TGG | + | chr3.1:66087798-66087817 | MS.gene70420:CDS | 30.0% |
| ! | TTAATTTGGATCCTAATAGG+TGG | + | chr3.1:66088066-66088085 | MS.gene70420:intron | 30.0% |
| GACTCTTCAACTAGAATACT+TGG | + | chr3.1:66087720-66087739 | MS.gene70420:CDS | 35.0% | |
| GTTTAGTAGTAATTGGCCAA+TGG | - | chr3.1:66087759-66087778 | None:intergenic | 35.0% | |
| TATGGTGTGGAAAAATCTGT+TGG | + | chr3.1:66087471-66087490 | MS.gene70420:CDS | 35.0% | |
| TCAATTACTTGATTCACTGC+AGG | - | chr3.1:66087952-66087971 | None:intergenic | 35.0% | |
| TCACCTGGTTTAGTAGTAAT+TGG | - | chr3.1:66087766-66087785 | None:intergenic | 35.0% | |
| TTGGCCAATGGATCAAAATA+AGG | - | chr3.1:66087747-66087766 | None:intergenic | 35.0% | |
| ! | ATAGGGTATTTAACTTCACC+TGG | - | chr3.1:66087781-66087800 | None:intergenic | 35.0% |
| !! | ATTGCAGAAGCACTTAAACT+TGG | + | chr3.1:66087501-66087520 | MS.gene70420:CDS | 35.0% |
| !!! | GTATACATGCTTGTTTTTGC+AGG | + | chr3.1:66087698-66087717 | MS.gene70420:intron | 35.0% |
| !!! | TGGACCTTATTTTGATCCAT+TGG | + | chr3.1:66087740-66087759 | MS.gene70420:CDS | 35.0% |
| AAAGAATTGGCTGATGCACA+TGG | + | chr3.1:66088223-66088242 | MS.gene70420:CDS | 40.0% | |
| ATTACTTGATTCACTGCAGG+AGG | - | chr3.1:66087949-66087968 | None:intergenic | 40.0% | |
| CATCAAGAGAGACTCATCAA+AGG | + | chr3.1:66088535-66088554 | MS.gene70420:CDS | 40.0% | |
| CTTTGATGAGTCTCTCTTGA+TGG | - | chr3.1:66088537-66088556 | None:intergenic | 40.0% | |
| GCATGGACCAACTTCTAATT+TGG | + | chr3.1:66088289-66088308 | MS.gene70420:intron | 40.0% | |
| TAATAGGTGGAAGTCAACCT+TGG | + | chr3.1:66088079-66088098 | MS.gene70420:intron | 40.0% | |
| TAGATCATCAAGAAGAGGCT+TGG | - | chr3.1:66088566-66088585 | None:intergenic | 40.0% | |
| TCCCATAGATCATCAAGAAG+AGG | - | chr3.1:66088571-66088590 | None:intergenic | 40.0% | |
| TGGCCAATTACTACTAAACC+AGG | + | chr3.1:66087760-66087779 | MS.gene70420:CDS | 40.0% | |
| TGTGGTTAAGAGCTATGACA+AGG | + | chr3.1:66088438-66088457 | MS.gene70420:CDS | 40.0% | |
| TTAAGAGCTATGACAAGGAG+AGG | + | chr3.1:66088443-66088462 | MS.gene70420:CDS | 40.0% | |
| TTACTTGATTCACTGCAGGA+GGG | - | chr3.1:66087948-66087967 | None:intergenic | 40.0% | |
| TTTGGCAGATTTGTCTTCGA+TGG | + | chr3.1:66088392-66088411 | MS.gene70420:intron | 40.0% | |
| ! | AACCTTGTTTGATGGCTTCA+AGG | - | chr3.1:66087425-66087444 | None:intergenic | 40.0% |
| ! | AAGGTTGACTTCCACCTATT+AGG | - | chr3.1:66088080-66088099 | None:intergenic | 40.0% |
| ! | AATACCCTTTTCCTTGCAGA+AGG | - | chr3.1:66088128-66088147 | None:intergenic | 40.0% |
| ! | AGCCTCTTCTTGATGATCTA+TGG | + | chr3.1:66088566-66088585 | MS.gene70420:CDS | 40.0% |
| ! | CTTTGATTGGTCATTGACTG+AGG | + | chr3.1:66088486-66088505 | MS.gene70420:CDS | 40.0% |
| ! | GCCTCTTCTTGATGATCTAT+GGG | + | chr3.1:66088567-66088586 | MS.gene70420:CDS | 40.0% |
| !! | TGAGCAAGGATTGACTTTTG+TGG | + | chr3.1:66088420-66088439 | MS.gene70420:CDS | 40.0% |
| ACATGGCAAGACTATAGCTC+AGG | + | chr3.1:66088240-66088259 | MS.gene70420:CDS | 45.0% | |
| AGAGCCTTCTGCAAGGAAAA+GGG | + | chr3.1:66088121-66088140 | MS.gene70420:CDS | 45.0% | |
| CTGCTCTGCAGAAATCTCTA+AGG | + | chr3.1:66087601-66087620 | MS.gene70420:CDS | 45.0% | |
| CTTAGAGATTTCTGCAGAGC+AGG | - | chr3.1:66087603-66087622 | None:intergenic | 45.0% | |
| CTTCGATGGCTATATGAGCA+AGG | + | chr3.1:66088406-66088425 | MS.gene70420:CDS | 45.0% | |
| GAAACTTAGAGCCTTCTGCA+AGG | + | chr3.1:66088114-66088133 | MS.gene70420:CDS | 45.0% | |
| GTCCTTGAAGCCATCAAACA+AGG | + | chr3.1:66087420-66087439 | MS.gene70420:CDS | 45.0% | |
| GTTTCTCTTGTTGCCATCCA+AGG | - | chr3.1:66088099-66088118 | None:intergenic | 45.0% | |
| TAGAGCCTTCTGCAAGGAAA+AGG | + | chr3.1:66088120-66088139 | MS.gene70420:CDS | 45.0% | |
| TGAGAAGTTGCTAGCTCCAA+TGG | - | chr3.1:66087887-66087906 | None:intergenic | 45.0% | |
| TTTGATGCAGCTGCTGCATA+TGG | + | chr3.1:66087453-66087472 | MS.gene70420:CDS | 45.0% | |
| ! | GAAAGGTGTGTGGACATCAT+TGG | + | chr3.1:66087831-66087850 | MS.gene70420:CDS | 45.0% |
| ! | GTAACCTTACTGGTTGCTTC+CGG | - | chr3.1:66087387-66087406 | None:intergenic | 45.0% |
| ! | TCATCAAGAAGAGGCTTGGT+TGG | - | chr3.1:66088562-66088581 | None:intergenic | 45.0% |
| !! | CAGGGGTGCTAATTTAGTGA+TGG | + | chr3.1:66088186-66088205 | MS.gene70420:CDS | 45.0% |
| !! | CTGCAAGGAAAAGGGTATTG+TGG | + | chr3.1:66088129-66088148 | MS.gene70420:CDS | 45.0% |
| !!! | AATTATTGATTAAATTAATT+TGG | + | chr3.1:66088052-66088071 | MS.gene70420:intron | 5.0% |
| ACAACGGAAAATGCCGGTGA+TGG | + | chr3.1:66087347-66087366 | MS.gene70420:CDS | 50.0% | |
| ACCTTTCCTAAGGGGTGAGA+AGG | - | chr3.1:66088161-66088180 | None:intergenic | 50.0% | |
| AGAGCAGGAACAATGAGCTC+AGG | - | chr3.1:66087588-66087607 | None:intergenic | 50.0% | |
| AGGACTACTTCAGGGACTGT+AGG | - | chr3.1:66087312-66087331 | None:intergenic | 50.0% | |
| AGGTGGAAGTCAACCTTGGA+TGG | + | chr3.1:66088083-66088102 | MS.gene70420:intron | 50.0% | |
| CAACGGAAAATGCCGGTGAT+GGG | + | chr3.1:66087348-66087367 | MS.gene70420:CDS | 50.0% | |
| CCTGCTAGCACCTTTCCTAA+GGG | - | chr3.1:66088170-66088189 | None:intergenic | 50.0% | |
| CTGCTAGCACCTTTCCTAAG+GGG | - | chr3.1:66088169-66088188 | None:intergenic | 50.0% | |
| CTTGGTCTCACCAAAGCCAT+TGG | + | chr3.1:66087868-66087887 | MS.gene70420:CDS | 50.0% | |
| GTTGCTAGCTCCAATGGCTT+TGG | - | chr3.1:66087881-66087900 | None:intergenic | 50.0% | |
| TAACTGCCTTCTCACCCCTT+AGG | + | chr3.1:66088152-66088171 | MS.gene70420:CDS | 50.0% | |
| TTGATTCACTGCAGGAGGGA+TGG | - | chr3.1:66087944-66087963 | None:intergenic | 50.0% | |
| !! | CCTTAGGAAAGGTGCTAGCA+GGG | + | chr3.1:66088168-66088187 | MS.gene70420:CDS | 50.0% |
| !! | CTTAGGAAAGGTGCTAGCAG+GGG | + | chr3.1:66088169-66088188 | MS.gene70420:CDS | 50.0% |
| AGGAGGGGAGGACTACTTCA+GGG | - | chr3.1:66087320-66087339 | None:intergenic | 55.0% | |
| CACCGGACAACGGAAAATGC+CGG | + | chr3.1:66087341-66087360 | MS.gene70420:CDS | 55.0% | |
| CCCTGCTAGCACCTTTCCTA+AGG | - | chr3.1:66088171-66088190 | None:intergenic | 55.0% | |
| GCCTTCTCACCCCTTAGGAA+AGG | + | chr3.1:66088157-66088176 | MS.gene70420:CDS | 55.0% | |
| TGCAGCTGCTGCATATGGTG+TGG | + | chr3.1:66087458-66087477 | MS.gene70420:CDS | 55.0% | |
| TGCAGGAGGGATGGTTGCAA+AGG | - | chr3.1:66087935-66087954 | None:intergenic | 55.0% | |
| ! | CACCGGCATTTTCCGTTGTC+CGG | - | chr3.1:66087346-66087365 | None:intergenic | 55.0% |
| ! | CATTTTCCGTTGTCCGGTGG+AGG | - | chr3.1:66087340-66087359 | None:intergenic | 55.0% |
| ! | CTTACTGGTTGCTTCCGGTG+CGG | - | chr3.1:66087382-66087401 | None:intergenic | 55.0% |
| !! | AAAATGCCGGTGATGGGCCT+CGG | + | chr3.1:66087354-66087373 | MS.gene70420:CDS | 55.0% |
| !! | CCCTTAGGAAAGGTGCTAGC+AGG | + | chr3.1:66088167-66088186 | MS.gene70420:CDS | 55.0% |
| CGCACCGGAAGCAACCAGTA+AGG | + | chr3.1:66087380-66087399 | MS.gene70420:CDS | 60.0% | |
| GAGGAGGGGAGGACTACTTC+AGG | - | chr3.1:66087321-66087340 | None:intergenic | 60.0% | |
| TTCCGTTGTCCGGTGGAGGA+GGG | - | chr3.1:66087336-66087355 | None:intergenic | 60.0% | |
| TTTCCGTTGTCCGGTGGAGG+AGG | - | chr3.1:66087337-66087356 | None:intergenic | 60.0% | |
| ! | CGGCATTTTCCGTTGTCCGG+TGG | - | chr3.1:66087343-66087362 | None:intergenic | 60.0% |
| GTAGTCCTCCCCTCCTCCAC+CGG | + | chr3.1:66087324-66087343 | MS.gene70420:CDS | 65.0% | |
| TCCCCTCCTCCACCGGACAA+CGG | + | chr3.1:66087331-66087350 | MS.gene70420:CDS | 65.0% | |
| TCCGTTGTCCGGTGGAGGAG+GGG | - | chr3.1:66087335-66087354 | None:intergenic | 65.0% | |
| GTTGTCCGGTGGAGGAGGGG+AGG | - | chr3.1:66087332-66087351 | None:intergenic | 70.0% | |
| ! | TTGCTTCCGGTGCGGTGCCG+AGG | - | chr3.1:66087374-66087393 | None:intergenic | 70.0% |
| GATGGGCCTCGGCACCGCAC+CGG | + | chr3.1:66087365-66087384 | MS.gene70420:CDS | 75.0% | |
| ! | GCGGTGCCGAGGCCCATCAC+CGG | - | chr3.1:66087363-66087382 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 66087297 | 66088600 | 66087297 | ID=MS.gene70420 |
| chr3.1 | mRNA | 66087297 | 66088600 | 66087297 | ID=MS.gene70420.t1;Parent=MS.gene70420 |
| chr3.1 | exon | 66087297 | 66087622 | 66087297 | ID=MS.gene70420.t1.exon1;Parent=MS.gene70420.t1 |
| chr3.1 | CDS | 66087297 | 66087622 | 66087297 | ID=cds.MS.gene70420.t1;Parent=MS.gene70420.t1 |
| chr3.1 | exon | 66087720 | 66087963 | 66087720 | ID=MS.gene70420.t1.exon2;Parent=MS.gene70420.t1 |
| chr3.1 | CDS | 66087720 | 66087963 | 66087720 | ID=cds.MS.gene70420.t1;Parent=MS.gene70420.t1 |
| chr3.1 | exon | 66088085 | 66088261 | 66088085 | ID=MS.gene70420.t1.exon3;Parent=MS.gene70420.t1 |
| chr3.1 | CDS | 66088085 | 66088261 | 66088085 | ID=cds.MS.gene70420.t1;Parent=MS.gene70420.t1 |
| chr3.1 | exon | 66088400 | 66088600 | 66088400 | ID=MS.gene70420.t1.exon4;Parent=MS.gene70420.t1 |
| chr3.1 | CDS | 66088400 | 66088600 | 66088400 | ID=cds.MS.gene70420.t1;Parent=MS.gene70420.t1 |
| Gene Sequence |
| Protein sequence |
