Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071843.t1 | XP_003594836.1 | 99.4 | 157 | 1 | 0 | 1 | 157 | 1 | 157 | 1.10E-82 | 315.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071843.t1 | E6YBW4 | 77.7 | 157 | 35 | 0 | 1 | 157 | 1 | 157 | 1.5e-64 | 246.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071843.t1 | G7IMZ2 | 99.4 | 157 | 1 | 0 | 1 | 157 | 1 | 157 | 7.9e-83 | 315.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene071843 | -0.807652 | 4.55E-50 | -1.69E-46 |
| MS.gene049217 | MS.gene071843 | -0.807243 | 5.56E-50 | -1.69E-46 |
| MS.gene049222 | MS.gene071843 | -0.814158 | 1.78E-51 | -1.69E-46 |
| MS.gene049232 | MS.gene071843 | -0.86152 | 9.69E-64 | -1.69E-46 |
| MS.gene049259 | MS.gene071843 | 0.821786 | 3.35E-53 | -1.69E-46 |
| MS.gene049320 | MS.gene071843 | -0.808462 | 3.06E-50 | -1.69E-46 |
| MS.gene049483 | MS.gene071843 | 0.816417 | 5.59E-52 | -1.69E-46 |
| MS.gene049611 | MS.gene071843 | 0.835294 | 1.82E-56 | -1.69E-46 |
| MS.gene049633 | MS.gene071843 | -0.802158 | 6.41E-49 | -1.69E-46 |
| MS.gene049634 | MS.gene071843 | 0.854149 | 1.49E-61 | -1.69E-46 |
| MS.gene04986 | MS.gene071843 | 0.801142 | 1.04E-48 | -1.69E-46 |
| MS.gene050082 | MS.gene071843 | 0.814526 | 1.47E-51 | -1.69E-46 |
| MS.gene05010 | MS.gene071843 | -0.808803 | 2.59E-50 | -1.69E-46 |
| MS.gene050170 | MS.gene071843 | -0.820967 | 5.18E-53 | -1.69E-46 |
| MS.gene050216 | MS.gene071843 | -0.876512 | 1.32E-68 | -1.69E-46 |
| MS.gene050323 | MS.gene071843 | -0.838302 | 3.12E-57 | -1.69E-46 |
| MS.gene050380 | MS.gene071843 | 0.833954 | 3.96E-56 | -1.69E-46 |
| MS.gene050381 | MS.gene071843 | 0.886871 | 2.35E-72 | -1.69E-46 |
| MS.gene050496 | MS.gene071843 | -0.830995 | 2.15E-55 | -1.69E-46 |
| MS.gene050504 | MS.gene071843 | -0.848314 | 6.63E-60 | -1.69E-46 |
| MS.gene050577 | MS.gene071843 | -0.809784 | 1.59E-50 | -1.69E-46 |
| MS.gene050612 | MS.gene071843 | -0.841719 | 4.01E-58 | -1.69E-46 |
| MS.gene050675 | MS.gene071843 | -0.812325 | 4.49E-51 | -1.69E-46 |
| MS.gene05079 | MS.gene071843 | 0.800788 | 1.22E-48 | -1.69E-46 |
| MS.gene05080 | MS.gene071843 | -0.808258 | 3.38E-50 | -1.69E-46 |
| MS.gene050868 | MS.gene071843 | -0.872946 | 2.15E-67 | -1.69E-46 |
| MS.gene050871 | MS.gene071843 | -0.876007 | 1.97E-68 | -1.69E-46 |
| MS.gene050883 | MS.gene071843 | 0.872474 | 3.09E-67 | -1.69E-46 |
| MS.gene050904 | MS.gene071843 | -0.804297 | 2.31E-49 | -1.69E-46 |
| MS.gene050986 | MS.gene071843 | -0.812861 | 3.43E-51 | -1.69E-46 |
| MS.gene051044 | MS.gene071843 | 0.83308 | 6.55E-56 | -1.69E-46 |
| MS.gene05104 | MS.gene071843 | 0.839144 | 1.89E-57 | -1.69E-46 |
| MS.gene051127 | MS.gene071843 | 0.878159 | 3.52E-69 | -1.69E-46 |
| MS.gene051138 | MS.gene071843 | 0.8556 | 5.66E-62 | -1.69E-46 |
| MS.gene051268 | MS.gene071843 | -0.835985 | 1.22E-56 | -1.69E-46 |
| MS.gene051364 | MS.gene071843 | 0.820913 | 5.34E-53 | -1.69E-46 |
| MS.gene051366 | MS.gene071843 | 0.809772 | 1.60E-50 | -1.69E-46 |
| MS.gene051410 | MS.gene071843 | 0.808697 | 2.73E-50 | -1.69E-46 |
| MS.gene05141 | MS.gene071843 | 0.840568 | 8.04E-58 | -1.69E-46 |
| MS.gene051509 | MS.gene071843 | -0.803333 | 3.67E-49 | -1.69E-46 |
| MS.gene051632 | MS.gene071843 | 0.814331 | 1.63E-51 | -1.69E-46 |
| MS.gene051651 | MS.gene071843 | 0.81164 | 6.33E-51 | -1.69E-46 |
| MS.gene051863 | MS.gene071843 | 0.84184 | 3.72E-58 | -1.69E-46 |
| MS.gene051874 | MS.gene071843 | -0.801256 | 9.81E-49 | -1.69E-46 |
| MS.gene051946 | MS.gene071843 | 0.849019 | 4.23E-60 | -1.69E-46 |
| MS.gene052066 | MS.gene071843 | -0.814136 | 1.80E-51 | -1.69E-46 |
| MS.gene052164 | MS.gene071843 | -0.828977 | 6.66E-55 | -1.69E-46 |
| MS.gene05234 | MS.gene071843 | 0.803827 | 2.89E-49 | -1.69E-46 |
| MS.gene052482 | MS.gene071843 | -0.834385 | 3.09E-56 | -1.69E-46 |
| MS.gene052486 | MS.gene071843 | -0.835625 | 1.51E-56 | -1.69E-46 |
| MS.gene052494 | MS.gene071843 | 0.805527 | 1.28E-49 | -1.69E-46 |
| MS.gene052566 | MS.gene071843 | 0.802331 | 5.90E-49 | -1.69E-46 |
| MS.gene052802 | MS.gene071843 | 0.828794 | 7.38E-55 | -1.69E-46 |
| MS.gene052966 | MS.gene071843 | -0.825186 | 5.38E-54 | -1.69E-46 |
| MS.gene053069 | MS.gene071843 | 0.815436 | 9.25E-52 | -1.69E-46 |
| MS.gene053203 | MS.gene071843 | -0.828659 | 7.95E-55 | -1.69E-46 |
| MS.gene053406 | MS.gene071843 | 0.80067 | 1.29E-48 | -1.69E-46 |
| MS.gene053420 | MS.gene071843 | 0.874902 | 4.70E-68 | -1.69E-46 |
| MS.gene053421 | MS.gene071843 | 0.814667 | 1.37E-51 | -1.69E-46 |
| MS.gene053488 | MS.gene071843 | 0.838097 | 3.52E-57 | -1.69E-46 |
| MS.gene053561 | MS.gene071843 | 0.835857 | 1.31E-56 | -1.69E-46 |
| MS.gene053633 | MS.gene071843 | -0.807409 | 5.13E-50 | -1.69E-46 |
| MS.gene053826 | MS.gene071843 | 0.829488 | 5.00E-55 | -1.69E-46 |
| MS.gene054003 | MS.gene071843 | -0.820232 | 7.65E-53 | -1.69E-46 |
| MS.gene054054 | MS.gene071843 | 0.838096 | 3.52E-57 | -1.69E-46 |
| MS.gene054056 | MS.gene071843 | 0.812058 | 5.13E-51 | -1.69E-46 |
| MS.gene054595 | MS.gene071843 | -0.83101 | 2.13E-55 | -1.69E-46 |
| MS.gene054616 | MS.gene071843 | 0.813103 | 3.03E-51 | -1.69E-46 |
| MS.gene054706 | MS.gene071843 | 0.828945 | 6.78E-55 | -1.69E-46 |
| MS.gene054707 | MS.gene071843 | 0.836543 | 8.80E-57 | -1.69E-46 |
| MS.gene054708 | MS.gene071843 | 0.831349 | 1.76E-55 | -1.69E-46 |
| MS.gene05470 | MS.gene071843 | -0.812814 | 3.51E-51 | -1.69E-46 |
| MS.gene054954 | MS.gene071843 | -0.821931 | 3.11E-53 | -1.69E-46 |
| MS.gene055124 | MS.gene071843 | 0.888717 | 4.61E-73 | -1.69E-46 |
| MS.gene055139 | MS.gene071843 | 0.837344 | 5.49E-57 | -1.69E-46 |
| MS.gene055154 | MS.gene071843 | 0.854343 | 1.31E-61 | -1.69E-46 |
| MS.gene055202 | MS.gene071843 | 0.883187 | 5.55E-71 | -1.69E-46 |
| MS.gene055240 | MS.gene071843 | 0.802292 | 6.01E-49 | -1.69E-46 |
| MS.gene055502 | MS.gene071843 | -0.832401 | 9.66E-56 | -1.69E-46 |
| MS.gene055577 | MS.gene071843 | -0.823543 | 1.31E-53 | -1.69E-46 |
| MS.gene055748 | MS.gene071843 | -0.800548 | 1.37E-48 | -1.69E-46 |
| MS.gene055750 | MS.gene071843 | -0.820942 | 5.25E-53 | -1.69E-46 |
| MS.gene055751 | MS.gene071843 | -0.813628 | 2.32E-51 | -1.69E-46 |
| MS.gene05589 | MS.gene071843 | -0.85506 | 8.12E-62 | -1.69E-46 |
| MS.gene055942 | MS.gene071843 | 0.808322 | 3.28E-50 | -1.69E-46 |
| MS.gene055959 | MS.gene071843 | 0.811496 | 6.80E-51 | -1.69E-46 |
| MS.gene056224 | MS.gene071843 | 0.833071 | 6.58E-56 | -1.69E-46 |
| MS.gene056298 | MS.gene071843 | 0.825599 | 4.29E-54 | -1.69E-46 |
| MS.gene056309 | MS.gene071843 | 0.804049 | 2.60E-49 | -1.69E-46 |
| MS.gene056310 | MS.gene071843 | 0.870714 | 1.18E-66 | -1.69E-46 |
| MS.gene056311 | MS.gene071843 | 0.872583 | 2.84E-67 | -1.69E-46 |
| MS.gene056313 | MS.gene071843 | 0.875458 | 3.03E-68 | -1.69E-46 |
| MS.gene056387 | MS.gene071843 | -0.819817 | 9.52E-53 | -1.69E-46 |
| MS.gene056504 | MS.gene071843 | -0.872544 | 2.93E-67 | -1.69E-46 |
| MS.gene056510 | MS.gene071843 | -0.822419 | 2.39E-53 | -1.69E-46 |
| MS.gene056856 | MS.gene071843 | 0.827366 | 1.63E-54 | -1.69E-46 |
| MS.gene056857 | MS.gene071843 | 0.805138 | 1.54E-49 | -1.69E-46 |
| MS.gene057014 | MS.gene071843 | 0.856347 | 3.42E-62 | -1.69E-46 |
| MS.gene057086 | MS.gene071843 | -0.814951 | 1.19E-51 | -1.69E-46 |
| MS.gene057291 | MS.gene071843 | 0.810709 | 1.01E-50 | -1.69E-46 |
| MS.gene057293 | MS.gene071843 | 0.803042 | 4.21E-49 | -1.69E-46 |
| MS.gene057473 | MS.gene071843 | 0.807401 | 5.15E-50 | -1.69E-46 |
| MS.gene057618 | MS.gene071843 | 0.804148 | 2.48E-49 | -1.69E-46 |
| MS.gene057768 | MS.gene071843 | 0.838064 | 3.59E-57 | -1.69E-46 |
| MS.gene058041 | MS.gene071843 | -0.831037 | 2.09E-55 | -1.69E-46 |
| MS.gene058068 | MS.gene071843 | 0.82892 | 6.87E-55 | -1.69E-46 |
| MS.gene058093 | MS.gene071843 | 0.844869 | 5.79E-59 | -1.69E-46 |
| MS.gene058099 | MS.gene071843 | 0.879896 | 8.56E-70 | -1.69E-46 |
| MS.gene058108 | MS.gene071843 | 0.905895 | 2.65E-80 | -1.69E-46 |
| MS.gene058109 | MS.gene071843 | 0.900079 | 1.05E-77 | -1.69E-46 |
| MS.gene058111 | MS.gene071843 | 0.889225 | 2.93E-73 | -1.69E-46 |
| MS.gene058117 | MS.gene071843 | 0.844625 | 6.73E-59 | -1.69E-46 |
| MS.gene058157 | MS.gene071843 | -0.845637 | 3.59E-59 | -1.69E-46 |
| MS.gene058170 | MS.gene071843 | 0.844846 | 5.87E-59 | -1.69E-46 |
| MS.gene058171 | MS.gene071843 | 0.997202 | 1.58E-238 | -1.69E-46 |
| MS.gene058173 | MS.gene071843 | 0.99255 | 5.70E-194 | -1.69E-46 |
| MS.gene058174 | MS.gene071843 | 0.839992 | 1.14E-57 | -1.69E-46 |
| MS.gene058175 | MS.gene071843 | 0.998604 | 3.42E-270 | -1.69E-46 |
| MS.gene058176 | MS.gene071843 | 0.80938 | 1.95E-50 | -1.69E-46 |
| MS.gene058179 | MS.gene071843 | 0.866143 | 3.54E-65 | -1.69E-46 |
| MS.gene058180 | MS.gene071843 | 0.85251 | 4.40E-61 | -1.69E-46 |
| MS.gene058334 | MS.gene071843 | 0.811465 | 6.91E-51 | -1.69E-46 |
| MS.gene05837 | MS.gene071843 | -0.828085 | 1.09E-54 | -1.69E-46 |
| MS.gene058493 | MS.gene071843 | 0.837522 | 4.95E-57 | -1.69E-46 |
| MS.gene058494 | MS.gene071843 | 0.835649 | 1.48E-56 | -1.69E-46 |
| MS.gene058496 | MS.gene071843 | 0.801833 | 7.47E-49 | -1.69E-46 |
| MS.gene058840 | MS.gene071843 | 0.812647 | 3.82E-51 | -1.69E-46 |
| MS.gene05884 | MS.gene071843 | 0.804706 | 1.90E-49 | -1.69E-46 |
| MS.gene059022 | MS.gene071843 | -0.826473 | 2.66E-54 | -1.69E-46 |
| MS.gene059037 | MS.gene071843 | 0.864206 | 1.44E-64 | -1.69E-46 |
| MS.gene059038 | MS.gene071843 | 0.830315 | 3.15E-55 | -1.69E-46 |
| MS.gene059044 | MS.gene071843 | 0.833865 | 4.17E-56 | -1.69E-46 |
| MS.gene059045 | MS.gene071843 | 0.913205 | 8.06E-84 | -1.69E-46 |
| MS.gene05913 | MS.gene071843 | -0.804306 | 2.30E-49 | -1.69E-46 |
| MS.gene059223 | MS.gene071843 | 0.849625 | 2.87E-60 | -1.69E-46 |
| MS.gene059367 | MS.gene071843 | -0.829458 | 5.09E-55 | -1.69E-46 |
| MS.gene059690 | MS.gene071843 | 0.817895 | 2.60E-52 | -1.69E-46 |
| MS.gene059694 | MS.gene071843 | 0.817244 | 3.65E-52 | -1.69E-46 |
| MS.gene059697 | MS.gene071843 | 0.818217 | 2.20E-52 | -1.69E-46 |
| MS.gene059703 | MS.gene071843 | 0.827482 | 1.53E-54 | -1.69E-46 |
| MS.gene059865 | MS.gene071843 | -0.871169 | 8.38E-67 | -1.69E-46 |
| MS.gene059916 | MS.gene071843 | 0.814312 | 1.64E-51 | -1.69E-46 |
| MS.gene059943 | MS.gene071843 | 0.824006 | 1.02E-53 | -1.69E-46 |
| MS.gene059944 | MS.gene071843 | 0.804354 | 2.25E-49 | -1.69E-46 |
| MS.gene06000 | MS.gene071843 | -0.825174 | 5.41E-54 | -1.69E-46 |
| MS.gene060187 | MS.gene071843 | 0.858098 | 1.04E-62 | -1.69E-46 |
| MS.gene060482 | MS.gene071843 | -0.812156 | 4.89E-51 | -1.69E-46 |
| MS.gene060593 | MS.gene071843 | 0.814867 | 1.24E-51 | -1.69E-46 |
| MS.gene060595 | MS.gene071843 | 0.859709 | 3.43E-63 | -1.69E-46 |
| MS.gene060703 | MS.gene071843 | -0.803351 | 3.63E-49 | -1.69E-46 |
| MS.gene060762 | MS.gene071843 | -0.829997 | 3.76E-55 | -1.69E-46 |
| MS.gene060778 | MS.gene071843 | -0.862377 | 5.29E-64 | -1.69E-46 |
| MS.gene06077 | MS.gene071843 | -0.844312 | 8.17E-59 | -1.69E-46 |
| MS.gene060982 | MS.gene071843 | -0.832709 | 8.10E-56 | -1.69E-46 |
| MS.gene061044 | MS.gene071843 | 0.80296 | 4.38E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071843.t1 | MTR_2g035170 | 99.363 | 157 | 1 | 0 | 1 | 157 | 1 | 157 | 2.37e-112 | 315 |
| MS.gene071843.t1 | MTR_2g035100 | 60.510 | 157 | 62 | 0 | 1 | 157 | 1 | 157 | 3.63e-63 | 191 |
| MS.gene071843.t1 | MTR_2g035105 | 59.236 | 157 | 64 | 0 | 1 | 157 | 1 | 157 | 6.68e-62 | 188 |
| MS.gene071843.t1 | MTR_2g035150 | 58.599 | 157 | 65 | 0 | 1 | 157 | 1 | 157 | 9.68e-62 | 187 |
| MS.gene071843.t1 | MTR_2g035120 | 57.962 | 157 | 66 | 0 | 1 | 157 | 1 | 157 | 1.55e-59 | 182 |
| MS.gene071843.t1 | MTR_2g035130 | 56.688 | 157 | 68 | 0 | 1 | 157 | 1 | 157 | 3.96e-58 | 178 |
| MS.gene071843.t1 | MTR_6g033450 | 54.140 | 157 | 72 | 0 | 1 | 157 | 1 | 157 | 1.75e-54 | 169 |
| MS.gene071843.t1 | MTR_4g120970 | 54.140 | 157 | 72 | 0 | 1 | 157 | 1 | 157 | 1.75e-54 | 169 |
| MS.gene071843.t1 | MTR_2g035320 | 48.408 | 157 | 81 | 0 | 1 | 157 | 1 | 157 | 8.37e-50 | 157 |
| MS.gene071843.t1 | MTR_2g035190 | 47.771 | 157 | 82 | 0 | 1 | 157 | 1 | 157 | 3.07e-48 | 153 |
| MS.gene071843.t1 | MTR_2g035210 | 47.771 | 157 | 82 | 0 | 1 | 157 | 1 | 157 | 1.01e-47 | 152 |
| MS.gene071843.t1 | MTR_2g035220 | 49.315 | 146 | 74 | 0 | 12 | 157 | 12 | 157 | 3.32e-47 | 150 |
| MS.gene071843.t1 | MTR_2g035320 | 50.000 | 100 | 50 | 0 | 58 | 157 | 23 | 122 | 8.93e-31 | 107 |
| MS.gene071843.t1 | MTR_2g435310 | 36.184 | 152 | 94 | 2 | 5 | 155 | 7 | 156 | 2.83e-30 | 107 |
| MS.gene071843.t1 | MTR_4g120760 | 32.692 | 156 | 104 | 1 | 1 | 155 | 1 | 156 | 1.60e-28 | 103 |
| MS.gene071843.t1 | MTR_4g120760 | 32.692 | 156 | 104 | 1 | 1 | 155 | 1 | 156 | 1.77e-28 | 103 |
| MS.gene071843.t1 | MTR_4g120950 | 39.752 | 161 | 90 | 5 | 1 | 157 | 1 | 158 | 2.86e-25 | 95.1 |
| MS.gene071843.t1 | MTR_4g120940 | 56.164 | 73 | 32 | 0 | 85 | 157 | 47 | 119 | 1.37e-20 | 82.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGTACAACTTTAGCATTAT+TGG | 0.213546 | 2.2:-53739953 | MS.gene071843:CDS |
| TGTACAACTTTAGCATTATT+GGG | 0.243931 | 2.2:-53739952 | MS.gene071843:CDS |
| TGGATCACTTCCACAAGCTT+TGG | 0.317240 | 2.2:+53740592 | None:intergenic |
| ATTAAACACATTCAGTAATC+AGG | 0.345127 | 2.2:+53739728 | None:intergenic |
| CATTTGGTCCTTCAACCAAT+TGG | 0.345692 | 2.2:+53739885 | None:intergenic |
| GGATCACTTCCACAAGCTTT+GGG | 0.357297 | 2.2:+53740593 | None:intergenic |
| ATGGCTTTGAAGAGCCTAAC+AGG | 0.389381 | 2.2:+53740640 | None:intergenic |
| CTGCTGCTGTTCCTCCTGTT+AGG | 0.389483 | 2.2:-53740654 | MS.gene071843:CDS |
| CTTCAGTGAACTCAATACTT+TGG | 0.401346 | 2.2:+53740572 | None:intergenic |
| ATCAACATGTACATTCCTAA+TGG | 0.402440 | 2.2:+53739856 | None:intergenic |
| ACTTAAATTATAATCACCTT+TGG | 0.409104 | 2.2:+53739826 | None:intergenic |
| TTCCTAATGGACCCTCCATT+TGG | 0.409557 | 2.2:+53739869 | None:intergenic |
| GCTTGTTGAAGGGTACCTTT+TGG | 0.410083 | 2.2:-53739756 | MS.gene071843:CDS |
| CACTCTGTGTAGCACATACT+TGG | 0.421047 | 2.2:+53740000 | None:intergenic |
| CTTCCACAAGCTTTGGGAAG+AGG | 0.431558 | 2.2:+53740599 | None:intergenic |
| AGTGAGGAGGAGTTGAAAGC+TGG | 0.432811 | 2.2:-53739806 | MS.gene071843:CDS |
| GGACTTGTAAAGCTTGTTGA+AGG | 0.441945 | 2.2:-53739767 | MS.gene071843:CDS |
| GACCAAATGGAGGGTCCATT+AGG | 0.448487 | 2.2:-53739871 | MS.gene071843:CDS |
| GCTGGTAAAGCAAAGGTTGA+AGG | 0.449190 | 2.2:-53739788 | MS.gene071843:CDS |
| AAGAAGCTCACCACAGTTGA+AGG | 0.458485 | 2.2:-53740526 | MS.gene071843:intron |
| ACTGAAGGAACTGGTGGTGC+TGG | 0.463110 | 2.2:-53740556 | MS.gene071843:CDS |
| AGTAATCAGGATTTGCCAAA+AGG | 0.469472 | 2.2:+53739741 | None:intergenic |
| CTAACAGGAGGAACAGCAGC+AGG | 0.474433 | 2.2:+53740655 | None:intergenic |
| AGTCTCATTCAAGAGCCAAT+TGG | 0.492931 | 2.2:-53739900 | MS.gene071843:CDS |
| TTCAAGAGCCAATTGGTTGA+AGG | 0.493896 | 2.2:-53739893 | MS.gene071843:CDS |
| ATTGAGTTCACTGAAGGAAC+TGG | 0.500969 | 2.2:-53740565 | MS.gene071843:CDS |
| TAACCTCTTCCCAAAGCTTG+TGG | 0.518650 | 2.2:-53740602 | MS.gene071843:CDS |
| CACTGGATTGGCTGACACAT+TGG | 0.522999 | 2.2:-53739927 | MS.gene071843:CDS |
| TGATTATAATTTAAGTGAGG+AGG | 0.547716 | 2.2:-53739819 | MS.gene071843:CDS |
| CAATTGGTTGAAGGACCAAA+TGG | 0.561796 | 2.2:-53739884 | MS.gene071843:CDS |
| AACAGGAGGAACAGCAGCAG+GGG | 0.564864 | 2.2:+53740657 | None:intergenic |
| GTACAACTTTAGCATTATTG+GGG | 0.564914 | 2.2:-53739951 | MS.gene071843:CDS |
| CATCATGGGTGTACAAACTC+AGG | 0.570571 | 2.2:-53740689 | None:intergenic |
| CAAAGTATTGAGTTCACTGA+AGG | 0.574960 | 2.2:-53740571 | MS.gene071843:CDS |
| TAATTACTGACCTTCAACTG+TGG | 0.582513 | 2.2:+53740516 | None:intergenic |
| TAACAGGAGGAACAGCAGCA+GGG | 0.612336 | 2.2:+53740656 | None:intergenic |
| TTGGTTGAAGGACCAAATGG+AGG | 0.621672 | 2.2:-53739881 | MS.gene071843:CDS |
| GACTTGTAAAGCTTGTTGAA+GGG | 0.631158 | 2.2:-53739766 | MS.gene071843:CDS |
| TGGTTGAAGGACCAAATGGA+GGG | 0.640692 | 2.2:-53739880 | MS.gene071843:CDS |
| GTTGAAAGCTGGTAAAGCAA+AGG | 0.651458 | 2.2:-53739795 | MS.gene071843:CDS |
| CAAGTATGTGCTACACAGAG+TGG | 0.654201 | 2.2:-53739999 | MS.gene071843:CDS |
| AGGTGATTATAATTTAAGTG+AGG | 0.654716 | 2.2:-53739822 | MS.gene071843:CDS |
| TACAACTTTAGCATTATTGG+GGG | 0.677045 | 2.2:-53739950 | MS.gene071843:CDS |
| GTTATGAAACTCTAATGACA+TGG | 0.696174 | 2.2:+53740621 | None:intergenic |
| GAGTTCACTGAAGGAACTGG+TGG | 0.720921 | 2.2:-53740562 | MS.gene071843:CDS |
| GCTTTGAAGAGCCTAACAGG+AGG | 0.746272 | 2.2:+53740643 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAAAATAATGATCA+TGG | + | chr2.2:53740241-53740260 | None:intergenic | 10.0% |
| !! | AGTTAGTTAAATTAAATTAA+TGG | - | chr2.2:53740361-53740380 | MS.gene071843:intron | 10.0% |
| !!! | ATTTTCTCTTATTTTAATTG+AGG | + | chr2.2:53740017-53740036 | None:intergenic | 15.0% |
| !!! | TTTTTATAGAAAAATGTTAG+TGG | - | chr2.2:53740261-53740280 | MS.gene071843:intron | 15.0% |
| !! | ACTTAAATTATAATCACCTT+TGG | + | chr2.2:53740601-53740620 | None:intergenic | 20.0% |
| !! | TGGTTAATTCGTTAAATTAA+AGG | - | chr2.2:53740281-53740300 | MS.gene071843:intron | 20.0% |
| !! | TTCGTTAAATTAAAGGAATA+TGG | - | chr2.2:53740288-53740307 | MS.gene071843:intron | 20.0% |
| !!! | TATTTTAATTCCATGACTTT+AGG | - | chr2.2:53739938-53739957 | MS.gene071843:CDS | 20.0% |
| ! | AAGTTTACAACGATATAAGT+AGG | + | chr2.2:53740124-53740143 | None:intergenic | 25.0% |
| ! | AGGTGATTATAATTTAAGTG+AGG | - | chr2.2:53740602-53740621 | MS.gene071843:CDS | 25.0% |
| ! | ATATAAGTAGGTGCAAATTA+TGG | + | chr2.2:53740112-53740131 | None:intergenic | 25.0% |
| ! | TGATTATAATTTAAGTGAGG+AGG | - | chr2.2:53740605-53740624 | MS.gene071843:CDS | 25.0% |
| ! | TGTACAACTTTAGCATTATT+GGG | - | chr2.2:53740472-53740491 | MS.gene071843:intron | 25.0% |
| !!! | CATGTTGATTATTTTACCAA+AGG | - | chr2.2:53740582-53740601 | MS.gene071843:CDS | 25.0% |
| ATCAACATGTACATTCCTAA+TGG | + | chr2.2:53740571-53740590 | None:intergenic | 30.0% | |
| GTACAACTTTAGCATTATTG+GGG | - | chr2.2:53740473-53740492 | MS.gene071843:intron | 30.0% | |
| GTGTACAACTTTAGCATTAT+TGG | - | chr2.2:53740471-53740490 | MS.gene071843:intron | 30.0% | |
| GTTATGAAACTCTAATGACA+TGG | + | chr2.2:53739806-53739825 | None:intergenic | 30.0% | |
| TACAACTTTAGCATTATTGG+GGG | - | chr2.2:53740474-53740493 | MS.gene071843:intron | 30.0% | |
| TGAGTGAATTTGATGAATAC+AGG | - | chr2.2:53740393-53740412 | MS.gene071843:intron | 30.0% | |
| ! | GTCTTACTTGTGATGATTTT+TGG | + | chr2.2:53740184-53740203 | None:intergenic | 30.0% |
| AGACTCAAATCCTAAAGTCA+TGG | + | chr2.2:53739951-53739970 | None:intergenic | 35.0% | |
| AGTAATCAGGATTTGCCAAA+AGG | + | chr2.2:53740686-53740705 | None:intergenic | 35.0% | |
| CTTCAGTGAACTCAATACTT+TGG | + | chr2.2:53739855-53739874 | None:intergenic | 35.0% | |
| GTGAATTTGATGAATACAGG+TGG | - | chr2.2:53740396-53740415 | MS.gene071843:intron | 35.0% | |
| TAATTACTGACCTTCAACTG+TGG | + | chr2.2:53739911-53739930 | None:intergenic | 35.0% | |
| ! | TCATTTTACAGTTGTCTCAG+AGG | - | chr2.2:53740064-53740083 | MS.gene071843:intron | 35.0% |
| !! | CAAAGTATTGAGTTCACTGA+AGG | - | chr2.2:53739853-53739872 | MS.gene071843:CDS | 35.0% |
| !! | GACTTGTAAAGCTTGTTGAA+GGG | - | chr2.2:53740658-53740677 | MS.gene071843:CDS | 35.0% |
| AGTCTCATTCAAGAGCCAAT+TGG | - | chr2.2:53740524-53740543 | MS.gene071843:intron | 40.0% | |
| ATTGAGTTCACTGAAGGAAC+TGG | - | chr2.2:53739859-53739878 | MS.gene071843:CDS | 40.0% | |
| CAATTGGTTGAAGGACCAAA+TGG | - | chr2.2:53740540-53740559 | MS.gene071843:CDS | 40.0% | |
| CATTTGGTCCTTCAACCAAT+TGG | + | chr2.2:53740542-53740561 | None:intergenic | 40.0% | |
| CTTTGTTAGAGTGACAAGTG+AGG | - | chr2.2:53739969-53739988 | MS.gene071843:CDS | 40.0% | |
| GTTGAAAGCTGGTAAAGCAA+AGG | - | chr2.2:53740629-53740648 | MS.gene071843:CDS | 40.0% | |
| TTCAAGAGCCAATTGGTTGA+AGG | - | chr2.2:53740531-53740550 | MS.gene071843:CDS | 40.0% | |
| !! | GGACTTGTAAAGCTTGTTGA+AGG | - | chr2.2:53740657-53740676 | MS.gene071843:CDS | 40.0% |
| AAGAAGCTCACCACAGTTGA+AGG | - | chr2.2:53739898-53739917 | MS.gene071843:CDS | 45.0% | |
| CAAGTATGTGCTACACAGAG+TGG | - | chr2.2:53740425-53740444 | MS.gene071843:intron | 45.0% | |
| CACTCTGTGTAGCACATACT+TGG | + | chr2.2:53740427-53740446 | None:intergenic | 45.0% | |
| GCTGGTAAAGCAAAGGTTGA+AGG | - | chr2.2:53740636-53740655 | MS.gene071843:CDS | 45.0% | |
| GGATCACTTCCACAAGCTTT+GGG | + | chr2.2:53739834-53739853 | None:intergenic | 45.0% | |
| TAACCTCTTCCCAAAGCTTG+TGG | - | chr2.2:53739822-53739841 | MS.gene071843:CDS | 45.0% | |
| TGGATCACTTCCACAAGCTT+TGG | + | chr2.2:53739835-53739854 | None:intergenic | 45.0% | |
| TGGTTGAAGGACCAAATGGA+GGG | - | chr2.2:53740544-53740563 | MS.gene071843:CDS | 45.0% | |
| TTGGTTGAAGGACCAAATGG+AGG | - | chr2.2:53740543-53740562 | MS.gene071843:CDS | 45.0% | |
| ! | TTCCTAATGGACCCTCCATT+TGG | + | chr2.2:53740558-53740577 | None:intergenic | 45.0% |
| ! | TTTAGCATTATTGGGGGCAC+TGG | - | chr2.2:53740480-53740499 | MS.gene071843:intron | 45.0% |
| !! | ATGGCTTTGAAGAGCCTAAC+AGG | + | chr2.2:53739787-53739806 | None:intergenic | 45.0% |
| !!! | GCTTGTTGAAGGGTACCTTT+TGG | - | chr2.2:53740668-53740687 | MS.gene071843:CDS | 45.0% |
| AGTGAGGAGGAGTTGAAAGC+TGG | - | chr2.2:53740618-53740637 | MS.gene071843:CDS | 50.0% | |
| GACCAAATGGAGGGTCCATT+AGG | - | chr2.2:53740553-53740572 | MS.gene071843:CDS | 50.0% | |
| GAGTTCACTGAAGGAACTGG+TGG | - | chr2.2:53739862-53739881 | MS.gene071843:CDS | 50.0% | |
| TAACAGGAGGAACAGCAGCA+GGG | + | chr2.2:53739771-53739790 | None:intergenic | 50.0% | |
| !! | CACTGGATTGGCTGACACAT+TGG | - | chr2.2:53740497-53740516 | MS.gene071843:intron | 50.0% |
| !! | CATTATTGGGGGCACTGGAT+TGG | - | chr2.2:53740485-53740504 | MS.gene071843:intron | 50.0% |
| !! | CTTCCACAAGCTTTGGGAAG+AGG | + | chr2.2:53739828-53739847 | None:intergenic | 50.0% |
| !! | GCTTTGAAGAGCCTAACAGG+AGG | + | chr2.2:53739784-53739803 | None:intergenic | 50.0% |
| AACAGGAGGAACAGCAGCAG+GGG | + | chr2.2:53739770-53739789 | None:intergenic | 55.0% | |
| CTAACAGGAGGAACAGCAGC+AGG | + | chr2.2:53739772-53739791 | None:intergenic | 55.0% | |
| CTGCTGCTGTTCCTCCTGTT+AGG | - | chr2.2:53739770-53739789 | MS.gene071843:CDS | 55.0% | |
| ! | ACTGAAGGAACTGGTGGTGC+TGG | - | chr2.2:53739868-53739887 | MS.gene071843:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.2 | gene | 53739739 | 53740707 | 53739739 | ID=MS.gene071843 |
| chr2.2 | mRNA | 53739739 | 53740707 | 53739739 | ID=MS.gene071843.t1;Parent=MS.gene071843 |
| chr2.2 | exon | 53740527 | 53740707 | 53740527 | ID=MS.gene071843.t1.exon1;Parent=MS.gene071843.t1 |
| chr2.2 | CDS | 53740527 | 53740707 | 53740527 | ID=cds.MS.gene071843.t1;Parent=MS.gene071843.t1 |
| chr2.2 | exon | 53739739 | 53740031 | 53739739 | ID=MS.gene071843.t1.exon2;Parent=MS.gene071843.t1 |
| chr2.2 | CDS | 53739739 | 53740031 | 53739739 | ID=cds.MS.gene071843.t1;Parent=MS.gene071843.t1 |
| Gene Sequence |
| Protein sequence |
