AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene61739


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61739.t1 MTR_4g121110 97.222 144 1 1 1 141 1 144 2.86e-98 278
MS.gene61739.t1 MTR_7g112330 50.877 114 50 3 29 141 21 129 1.30e-30 107
MS.gene61739.t1 MTR_1g016170 63.265 49 18 0 26 74 183 231 5.50e-17 75.9
MS.gene61739.t1 MTR_5g020230 58.000 50 18 1 20 66 109 158 4.10e-13 64.3
MS.gene61739.t1 MTR_4g126350 68.421 38 12 0 22 59 178 215 5.55e-13 64.7
MS.gene61739.t1 MTR_2g038720 76.667 30 7 0 30 59 164 193 4.23e-12 62.0
MS.gene61739.t1 MTR_8g077510 64.865 37 13 0 30 66 125 161 5.03e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61739.t1 AT5G49300 54.362 149 49 6 2 141 1 139 1.73e-41 135
MS.gene61739.t1 AT3G06740 56.028 141 43 6 7 141 22 149 3.57e-40 132
MS.gene61739.t1 AT3G16870 42.675 157 43 5 27 141 39 190 1.55e-28 103
MS.gene61739.t1 AT4G16141 45.062 162 36 7 29 137 36 197 1.17e-26 99.0
MS.gene61739.t1 AT5G26930 67.442 43 13 1 30 71 26 68 1.81e-16 70.9
MS.gene61739.t1 AT5G56860 70.732 41 12 0 23 63 223 263 4.28e-15 71.2
MS.gene61739.t1 AT2G18380 50.000 66 29 1 30 91 92 157 1.18e-13 65.5
MS.gene61739.t1 AT4G36620 46.970 66 23 1 30 95 75 128 3.82e-13 64.3
MS.gene61739.t1 AT4G26150 77.419 31 7 0 30 60 199 229 5.16e-13 65.1
MS.gene61739.t1 AT3G50870 67.568 37 12 0 30 66 152 188 2.83e-12 62.8

Find 0 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGTTCATTTTTCATTTTC+AGG - 32473:15623-15642 MS.gene61739:CDS 20.0%
! AAGCAGAGATTATTGAATTT+AGG - 32473:16006-16025 MS.gene61739:intron 25.0%
! AGCAGAGATTATTGAATTTA+GGG - 32473:16007-16026 MS.gene61739:intron 25.0%
! GATTAGTGATTAATTACCTT+TGG + 32473:15771-15790 MS.gene61739:intergenic 25.0%
!! ATTGTTGTTATCTGATGAAA+CGG + 32473:15682-15701 MS.gene61739:intergenic 25.0%
TGTTTATGAATCGATCACAT+TGG - 32473:16037-16056 MS.gene61739:intron 30.0%
TTGTTGATGTCTCTTTCTTA+TGG - 32473:16093-16112 MS.gene61739:intron 30.0%
!!! TTTCATTTTCAGGTATCAGA+AGG - 32473:15633-15652 MS.gene61739:CDS 30.0%
AACGAAGAAGGAACAAGAAA+GGG - 32473:15931-15950 MS.gene61739:CDS 35.0%
AAGAAGAAGAGAGCGATATT+AGG - 32473:15892-15911 MS.gene61739:CDS 35.0%
AGAAGAAGAGAGCGATATTA+GGG - 32473:15893-15912 MS.gene61739:CDS 35.0%
CGATCACATTGGAAGAAATT+GGG - 32473:16048-16067 MS.gene61739:intron 35.0%
TCGATCACATTGGAAGAAAT+TGG - 32473:16047-16066 MS.gene61739:intron 35.0%
TTGAAGTGAAGTGTGTGATT+AGG + 32473:15562-15581 MS.gene61739:intergenic 35.0%
AAGGGGAAAAAGAGTAACAG+TGG - 32473:15949-15968 MS.gene61739:CDS 40.0%
ACGAAGAAGGAACAAGAAAG+GGG - 32473:15932-15951 MS.gene61739:CDS 40.0%
AGCAAAGGAAACAACGAAGA+AGG - 32473:15919-15938 MS.gene61739:CDS 40.0%
CAACGAAGAAGGAACAAGAA+AGG - 32473:15930-15949 MS.gene61739:CDS 40.0%
GAACTTCCAAAACTCCTCTA+TGG - 32473:15727-15746 MS.gene61739:CDS 40.0%
GCGATATTAGGGATAAGCAA+AGG - 32473:15904-15923 MS.gene61739:CDS 40.0%
GTTGTTATCTGATGAAACGG+CGG + 32473:15679-15698 MS.gene61739:intergenic 40.0%
TTAATTACCTTTGGTCCTGC+AGG + 32473:15762-15781 MS.gene61739:intergenic 40.0%
! CAATCAGCACAGGTTTTCTT+TGG + 32473:15705-15724 MS.gene61739:intergenic 40.0%
!! AAGAAAACCTGTGCTGATTG+CGG - 32473:15705-15724 MS.gene61739:CDS 40.0%
AAAAAGAGTAACAGTGGCGG+TGG - 32473:15955-15974 MS.gene61739:CDS 45.0%
GGGAAAAAGAGTAACAGTGG+CGG - 32473:15952-15971 MS.gene61739:CDS 45.0%
GGTGGTTGATCACAGTGATA+AGG - 32473:15506-15525 MS.gene61739:CDS 45.0%
AGTCACTGTGCAATGCTTGC+GGG - 32473:15857-15876 MS.gene61739:intron 50.0%
CAGTCACTGTGCAATGCTTG+CGG - 32473:15856-15875 MS.gene61739:intron 50.0%
TCCAAAACTCCTCTATGGCG+CGG - 32473:15732-15751 MS.gene61739:CDS 50.0%
AAAACTCCTCTATGGCGCGG+TGG - 32473:15735-15754 MS.gene61739:CDS 55.0%
AGTGGCGGTGGAAGCAAAGT+TGG - 32473:15967-15986 MS.gene61739:CDS 55.0%
CTGATGAAACGGCGGCGTTA+GGG + 32473:15671-15690 MS.gene61739:intergenic 55.0%
GGAAGTTCCGCAATCAGCAC+AGG + 32473:15715-15734 MS.gene61739:intergenic 55.0%
TCTGATGAAACGGCGGCGTT+AGG + 32473:15672-15691 MS.gene61739:intergenic 55.0%
TGTGCAATGCTTGCGGGATC+AGG - 32473:15863-15882 MS.gene61739:CDS 55.0%
! ACCGCGCCATAGAGGAGTTT+TGG + 32473:15736-15755 MS.gene61739:intergenic 55.0%
ATGCTTGCGGGATCAGGAGC+AGG - 32473:15869-15888 MS.gene61739:CDS 60.0%
CGGTGGTCCTGCAGGACCAA+AGG - 32473:15752-15771 MS.gene61739:CDS 65.0%
CTATGGCGCGGTGGTCCTGC+AGG - 32473:15744-15763 MS.gene61739:CDS 70.0%
GCAGGACCACCGCGCCATAG+AGG + 32473:15744-15763 MS.gene61739:intergenic 70.0%


Chromosome Type Strat End Strand Name
32473 gene 15504 16131 15504 ID=MS.gene61739
32473 mRNA 15504 16131 15504 ID=MS.gene61739.t1;Parent=MS.gene61739
32473 exon 16108 16131 16108 ID=MS.gene61739.t1.exon1;Parent=MS.gene61739.t1
32473 CDS 16108 16131 16108 ID=cds.MS.gene61739.t1;Parent=MS.gene61739.t1
32473 exon 15862 15990 15862 ID=MS.gene61739.t1.exon2;Parent=MS.gene61739.t1
32473 CDS 15862 15990 15862 ID=cds.MS.gene61739.t1;Parent=MS.gene61739.t1
32473 exon 15504 15776 15504 ID=MS.gene61739.t1.exon3;Parent=MS.gene61739.t1
32473 CDS 15504 15776 15504 ID=cds.MS.gene61739.t1;Parent=MS.gene61739.t1
Gene Sequence

>MS.gene61739

ATGGTGGTTGATCACAGTGATAAGGTAAGTGTTATTTGTTGATCAAATTAATTAACCTAATCACACACTTCACTTCAATCATTCATGATTTAATAGTTCAAATCACTAATCAATCTTGTTTTGTTCATTTTTCATTTTCAGGTATCAGAAGGTGAAGATTCAAACCCTAACGCCGCCGTTTCATCAGATAACAACAATCCAAAGAAAACCTGTGCTGATTGCGGAACTTCCAAAACTCCTCTATGGCGCGGTGGTCCTGCAGGACCAAAGGTAATTAATCACTAATCAATCGATTTGATGATTGATCACACTAATTTTGTTGTTGATCTGATCTAATATGTGATTAATTATGCAGTCACTGTGCAATGCTTGCGGGATCAGGAGCAGGAAGAAGAAGAGAGCGATATTAGGGATAAGCAAAGGAAACAACGAAGAAGGAACAAGAAAGGGGAAAAAGAGTAACAGTGGCGGTGGAAGCAAAGTTGGTGATAATTTGAACATGAAGCAGAGATTATTGAATTTAGGGAAAGAAGTGTTTATGAATCGATCACATTGGAAGAAATTGGGAGAAGATGAACAAGCTGCTGTGTTGTTGATGTCTCTTTCTTATGGATCTGTTTATGCCTAA

Protein sequence

>MS.gene61739.t1

MVVDHSDKVSEGEDSNPNAAVSSDNNNPKKTCADCGTSKTPLWRGGPAGPKSLCNACGIRSRKKKRAILGISKGNNEEGTRKGKKSNSGGGSKVGDNLNMKQRLLNLGKEVFMNRSHWKKLGEDEQAAVLLMSLSYGSVYA