Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065581.t1 | XP_013464342.1 | 96.8 | 216 | 7 | 0 | 1 | 216 | 1 | 216 | 1.70E-118 | 435.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065581.t1 | P46417 | 78.7 | 216 | 46 | 0 | 1 | 216 | 1 | 216 | 2.0e-99 | 363.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065581.t1 | A0A072V9B8 | 96.8 | 216 | 7 | 0 | 1 | 216 | 1 | 216 | 1.2e-118 | 435.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049151 | MS.gene065581 | 0.803699 | 3.08E-49 | -1.69E-46 |
| MS.gene049297 | MS.gene065581 | 0.803072 | 4.15E-49 | -1.69E-46 |
| MS.gene049346 | MS.gene065581 | 0.845686 | 3.48E-59 | -1.69E-46 |
| MS.gene049378 | MS.gene065581 | 0.839671 | 1.38E-57 | -1.69E-46 |
| MS.gene049426 | MS.gene065581 | 0.815128 | 1.08E-51 | -1.69E-46 |
| MS.gene049758 | MS.gene065581 | 0.864897 | 8.74E-65 | -1.69E-46 |
| MS.gene049910 | MS.gene065581 | 0.867349 | 1.46E-65 | -1.69E-46 |
| MS.gene049915 | MS.gene065581 | 0.836295 | 1.02E-56 | -1.69E-46 |
| MS.gene050168 | MS.gene065581 | 0.805535 | 1.27E-49 | -1.69E-46 |
| MS.gene050226 | MS.gene065581 | 0.848622 | 5.45E-60 | -1.69E-46 |
| MS.gene050227 | MS.gene065581 | 0.839947 | 1.17E-57 | -1.69E-46 |
| MS.gene050228 | MS.gene065581 | 0.8452 | 4.71E-59 | -1.69E-46 |
| MS.gene050301 | MS.gene065581 | 0.850155 | 2.04E-60 | -1.69E-46 |
| MS.gene050558 | MS.gene065581 | 0.826317 | 2.90E-54 | -1.69E-46 |
| MS.gene050642 | MS.gene065581 | 0.811753 | 5.98E-51 | -1.69E-46 |
| MS.gene050820 | MS.gene065581 | 0.812191 | 4.80E-51 | -1.69E-46 |
| MS.gene05119 | MS.gene065581 | -0.822238 | 2.64E-53 | -1.69E-46 |
| MS.gene051582 | MS.gene065581 | 0.810837 | 9.45E-51 | -1.69E-46 |
| MS.gene051662 | MS.gene065581 | 0.825567 | 4.37E-54 | -1.69E-46 |
| MS.gene051663 | MS.gene065581 | 0.850757 | 1.38E-60 | -1.69E-46 |
| MS.gene051860 | MS.gene065581 | 0.826879 | 2.13E-54 | -1.69E-46 |
| MS.gene051983 | MS.gene065581 | 0.817149 | 3.83E-52 | -1.69E-46 |
| MS.gene052077 | MS.gene065581 | 0.833079 | 6.55E-56 | -1.69E-46 |
| MS.gene052176 | MS.gene065581 | 0.808645 | 2.80E-50 | -1.69E-46 |
| MS.gene052188 | MS.gene065581 | 0.802696 | 4.96E-49 | -1.69E-46 |
| MS.gene052253 | MS.gene065581 | 0.81747 | 3.24E-52 | -1.69E-46 |
| MS.gene052340 | MS.gene065581 | 0.814166 | 1.77E-51 | -1.69E-46 |
| MS.gene052376 | MS.gene065581 | 0.807218 | 5.63E-50 | -1.69E-46 |
| MS.gene053184 | MS.gene065581 | 0.814621 | 1.40E-51 | -1.69E-46 |
| MS.gene053494 | MS.gene065581 | 0.800581 | 1.35E-48 | -1.69E-46 |
| MS.gene053577 | MS.gene065581 | 0.808001 | 3.84E-50 | -1.69E-46 |
| MS.gene053836 | MS.gene065581 | 0.898261 | 6.31E-77 | -1.69E-46 |
| MS.gene053928 | MS.gene065581 | 0.814129 | 1.80E-51 | -1.69E-46 |
| MS.gene054473 | MS.gene065581 | 0.844827 | 5.94E-59 | -1.69E-46 |
| MS.gene054542 | MS.gene065581 | 0.80615 | 9.46E-50 | -1.69E-46 |
| MS.gene054930 | MS.gene065581 | 0.869881 | 2.22E-66 | -1.69E-46 |
| MS.gene054942 | MS.gene065581 | 0.819682 | 1.02E-52 | -1.69E-46 |
| MS.gene054983 | MS.gene065581 | 0.829504 | 4.96E-55 | -1.69E-46 |
| MS.gene055116 | MS.gene065581 | 0.81138 | 7.21E-51 | -1.69E-46 |
| MS.gene055277 | MS.gene065581 | 0.819318 | 1.24E-52 | -1.69E-46 |
| MS.gene055422 | MS.gene065581 | 0.833138 | 6.33E-56 | -1.69E-46 |
| MS.gene055428 | MS.gene065581 | 0.840506 | 8.35E-58 | -1.69E-46 |
| MS.gene055604 | MS.gene065581 | 0.811956 | 5.40E-51 | -1.69E-46 |
| MS.gene056407 | MS.gene065581 | 0.822134 | 2.79E-53 | -1.69E-46 |
| MS.gene056612 | MS.gene065581 | 0.819046 | 1.43E-52 | -1.69E-46 |
| MS.gene056723 | MS.gene065581 | 0.811343 | 7.34E-51 | -1.69E-46 |
| MS.gene056875 | MS.gene065581 | 0.819208 | 1.31E-52 | -1.69E-46 |
| MS.gene056979 | MS.gene065581 | 0.808906 | 2.46E-50 | -1.69E-46 |
| MS.gene05702 | MS.gene065581 | 0.830814 | 2.38E-55 | -1.69E-46 |
| MS.gene057121 | MS.gene065581 | 0.827251 | 1.73E-54 | -1.69E-46 |
| MS.gene057439 | MS.gene065581 | 0.801853 | 7.40E-49 | -1.69E-46 |
| MS.gene057469 | MS.gene065581 | 0.822323 | 2.52E-53 | -1.69E-46 |
| MS.gene057750 | MS.gene065581 | 0.822253 | 2.62E-53 | -1.69E-46 |
| MS.gene057754 | MS.gene065581 | 0.82569 | 4.08E-54 | -1.69E-46 |
| MS.gene058002 | MS.gene065581 | 0.850348 | 1.80E-60 | -1.69E-46 |
| MS.gene05811 | MS.gene065581 | 0.824084 | 9.77E-54 | -1.69E-46 |
| MS.gene058142 | MS.gene065581 | 0.822519 | 2.27E-53 | -1.69E-46 |
| MS.gene058710 | MS.gene065581 | 0.814824 | 1.27E-51 | -1.69E-46 |
| MS.gene058749 | MS.gene065581 | 0.827448 | 1.56E-54 | -1.69E-46 |
| MS.gene058845 | MS.gene065581 | 0.811325 | 7.41E-51 | -1.69E-46 |
| MS.gene05907 | MS.gene065581 | 0.834708 | 2.56E-56 | -1.69E-46 |
| MS.gene059124 | MS.gene065581 | 0.834899 | 2.29E-56 | -1.69E-46 |
| MS.gene059285 | MS.gene065581 | 0.811236 | 7.75E-51 | -1.69E-46 |
| MS.gene059518 | MS.gene065581 | 0.821016 | 5.05E-53 | -1.69E-46 |
| MS.gene059597 | MS.gene065581 | 0.812381 | 4.36E-51 | -1.69E-46 |
| MS.gene059688 | MS.gene065581 | 0.806223 | 9.13E-50 | -1.69E-46 |
| MS.gene059692 | MS.gene065581 | 0.82083 | 5.58E-53 | -1.69E-46 |
| MS.gene059695 | MS.gene065581 | 0.814569 | 1.44E-51 | -1.69E-46 |
| MS.gene05984 | MS.gene065581 | 0.828033 | 1.13E-54 | -1.69E-46 |
| MS.gene059894 | MS.gene065581 | 0.828888 | 7.00E-55 | -1.69E-46 |
| MS.gene06037 | MS.gene065581 | 0.821283 | 4.38E-53 | -1.69E-46 |
| MS.gene060476 | MS.gene065581 | 0.833728 | 4.51E-56 | -1.69E-46 |
| MS.gene061049 | MS.gene065581 | 0.866056 | 3.77E-65 | -1.69E-46 |
| MS.gene061113 | MS.gene065581 | 0.805403 | 1.36E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065581.t1 | MTR_2g070060 | 96.759 | 216 | 7 | 0 | 1 | 216 | 1 | 216 | 4.10e-158 | 436 |
| MS.gene065581.t1 | MTR_2g070070 | 84.722 | 216 | 33 | 0 | 1 | 216 | 1 | 216 | 3.29e-132 | 370 |
| MS.gene065581.t1 | MTR_2g070110 | 82.870 | 216 | 36 | 1 | 1 | 216 | 1 | 215 | 1.11e-128 | 362 |
| MS.gene065581.t1 | MTR_2g070180 | 64.953 | 214 | 75 | 0 | 1 | 214 | 1 | 214 | 1.33e-108 | 311 |
| MS.gene065581.t1 | MTR_2g070150 | 66.667 | 204 | 68 | 0 | 1 | 204 | 1 | 204 | 5.99e-106 | 303 |
| MS.gene065581.t1 | MTR_2g070140 | 66.162 | 198 | 67 | 0 | 17 | 214 | 1 | 198 | 4.55e-102 | 294 |
| MS.gene065581.t1 | MTR_2g070130 | 61.682 | 214 | 82 | 0 | 1 | 214 | 1 | 214 | 2.21e-101 | 293 |
| MS.gene065581.t1 | MTR_8g056940 | 64.319 | 213 | 76 | 0 | 1 | 213 | 1 | 213 | 4.96e-101 | 291 |
| MS.gene065581.t1 | MTR_2g070210 | 60.092 | 218 | 85 | 1 | 1 | 216 | 1 | 218 | 7.63e-96 | 278 |
| MS.gene065581.t1 | MTR_2g070200 | 61.321 | 212 | 80 | 2 | 1 | 210 | 1 | 212 | 1.18e-94 | 276 |
| MS.gene065581.t1 | MTR_3g467420 | 58.491 | 212 | 88 | 0 | 3 | 214 | 8 | 219 | 9.97e-94 | 273 |
| MS.gene065581.t1 | MTR_4g124130 | 60.000 | 210 | 83 | 1 | 1 | 209 | 1 | 210 | 1.58e-90 | 265 |
| MS.gene065581.t1 | MTR_3g467430 | 56.604 | 212 | 92 | 0 | 3 | 214 | 8 | 219 | 3.68e-90 | 264 |
| MS.gene065581.t1 | MTR_2g070120 | 54.067 | 209 | 96 | 0 | 1 | 209 | 1 | 209 | 2.15e-82 | 244 |
| MS.gene065581.t1 | MTR_5g076900 | 49.065 | 214 | 107 | 2 | 2 | 214 | 4 | 216 | 6.94e-72 | 218 |
| MS.gene065581.t1 | MTR_0186s0030 | 66.667 | 141 | 47 | 0 | 1 | 141 | 1 | 141 | 1.72e-63 | 194 |
| MS.gene065581.t1 | MTR_5g040430 | 36.697 | 218 | 131 | 2 | 2 | 212 | 5 | 222 | 4.12e-49 | 160 |
| MS.gene065581.t1 | MTR_5g037380 | 39.910 | 223 | 125 | 4 | 3 | 216 | 4 | 226 | 3.23e-47 | 155 |
| MS.gene065581.t1 | MTR_1g090060 | 40.092 | 217 | 125 | 3 | 4 | 216 | 3 | 218 | 6.41e-45 | 149 |
| MS.gene065581.t1 | MTR_8g087425 | 38.073 | 218 | 130 | 4 | 2 | 216 | 3 | 218 | 1.04e-44 | 149 |
| MS.gene065581.t1 | MTR_2g072120 | 91.549 | 71 | 6 | 0 | 47 | 117 | 1 | 71 | 1.38e-43 | 141 |
| MS.gene065581.t1 | MTR_4g019780 | 37.981 | 208 | 124 | 4 | 4 | 208 | 8 | 213 | 3.06e-42 | 142 |
| MS.gene065581.t1 | MTR_8g087410 | 39.320 | 206 | 119 | 5 | 1 | 202 | 2 | 205 | 5.36e-42 | 142 |
| MS.gene065581.t1 | MTR_4g019790 | 39.623 | 212 | 123 | 4 | 3 | 211 | 6 | 215 | 9.20e-42 | 141 |
| MS.gene065581.t1 | MTR_4g059730 | 38.426 | 216 | 122 | 5 | 1 | 209 | 1 | 212 | 1.51e-40 | 138 |
| MS.gene065581.t1 | MTR_1g090070 | 37.900 | 219 | 128 | 5 | 4 | 216 | 9 | 225 | 2.52e-40 | 137 |
| MS.gene065581.t1 | MTR_7g065720 | 35.909 | 220 | 129 | 5 | 4 | 216 | 7 | 221 | 4.23e-40 | 137 |
| MS.gene065581.t1 | MTR_6g080440 | 37.321 | 209 | 126 | 4 | 3 | 208 | 2 | 208 | 5.13e-40 | 137 |
| MS.gene065581.t1 | MTR_3g099757 | 38.164 | 207 | 125 | 3 | 3 | 207 | 4 | 209 | 5.96e-40 | 136 |
| MS.gene065581.t1 | MTR_7g065750 | 40.299 | 201 | 114 | 5 | 4 | 200 | 7 | 205 | 1.22e-39 | 135 |
| MS.gene065581.t1 | MTR_7g065660 | 37.209 | 215 | 130 | 4 | 5 | 216 | 8 | 220 | 1.70e-39 | 135 |
| MS.gene065581.t1 | MTR_7g065630 | 36.111 | 216 | 129 | 5 | 4 | 216 | 25 | 234 | 2.14e-39 | 135 |
| MS.gene065581.t1 | MTR_1g090100 | 41.089 | 202 | 114 | 3 | 11 | 208 | 9 | 209 | 3.68e-39 | 134 |
| MS.gene065581.t1 | MTR_7g065590 | 37.037 | 216 | 131 | 4 | 4 | 216 | 7 | 220 | 2.80e-38 | 132 |
| MS.gene065581.t1 | MTR_8g061950 | 35.849 | 212 | 127 | 3 | 3 | 205 | 4 | 215 | 7.54e-38 | 131 |
| MS.gene065581.t1 | MTR_7g065740 | 35.885 | 209 | 128 | 4 | 4 | 208 | 7 | 213 | 1.47e-37 | 130 |
| MS.gene065581.t1 | MTR_1g090090 | 39.713 | 209 | 121 | 4 | 4 | 208 | 3 | 210 | 3.60e-37 | 129 |
| MS.gene065581.t1 | MTR_7g065710 | 35.545 | 211 | 127 | 5 | 1 | 208 | 1 | 205 | 8.99e-37 | 128 |
| MS.gene065581.t1 | MTR_1g090150 | 36.364 | 209 | 127 | 4 | 4 | 208 | 3 | 209 | 3.17e-36 | 127 |
| MS.gene065581.t1 | MTR_7g065230 | 37.037 | 216 | 126 | 5 | 4 | 216 | 7 | 215 | 1.27e-35 | 125 |
| MS.gene065581.t1 | MTR_7g065265 | 36.538 | 208 | 127 | 4 | 4 | 208 | 7 | 212 | 2.22e-34 | 122 |
| MS.gene065581.t1 | MTR_7g065270 | 36.058 | 208 | 128 | 4 | 4 | 208 | 7 | 212 | 6.49e-34 | 121 |
| MS.gene065581.t1 | MTR_7g065680 | 35.577 | 208 | 125 | 5 | 4 | 208 | 7 | 208 | 6.84e-34 | 121 |
| MS.gene065581.t1 | MTR_7g065600 | 35.349 | 215 | 134 | 4 | 5 | 216 | 8 | 220 | 1.83e-33 | 120 |
| MS.gene065581.t1 | MTR_1g115195 | 34.615 | 208 | 128 | 4 | 4 | 205 | 3 | 208 | 2.50e-33 | 119 |
| MS.gene065581.t1 | MTR_7g065700 | 35.545 | 211 | 127 | 5 | 1 | 208 | 4 | 208 | 3.16e-33 | 119 |
| MS.gene065581.t1 | MTR_7g065290 | 36.058 | 208 | 128 | 4 | 4 | 208 | 7 | 212 | 1.26e-32 | 117 |
| MS.gene065581.t1 | MTR_7g065640 | 32.394 | 213 | 125 | 4 | 5 | 216 | 8 | 202 | 2.30e-30 | 111 |
| MS.gene065581.t1 | MTR_7g065260 | 37.129 | 202 | 122 | 4 | 4 | 202 | 14 | 213 | 5.35e-30 | 111 |
| MS.gene065581.t1 | MTR_4g043640 | 57.143 | 42 | 18 | 0 | 148 | 189 | 44 | 85 | 4.73e-13 | 62.8 |
| MS.gene065581.t1 | MTR_5g037390 | 41.860 | 86 | 48 | 1 | 30 | 113 | 13 | 98 | 8.54e-13 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065581.t1 | AT1G78380 | 64.019 | 214 | 77 | 0 | 1 | 214 | 1 | 214 | 1.73e-103 | 298 |
| MS.gene065581.t1 | AT1G17180 | 61.574 | 216 | 83 | 0 | 1 | 216 | 1 | 216 | 2.86e-102 | 295 |
| MS.gene065581.t1 | AT1G78370 | 60.930 | 215 | 84 | 0 | 1 | 215 | 1 | 215 | 2.37e-97 | 282 |
| MS.gene065581.t1 | AT1G17170 | 60.648 | 216 | 84 | 1 | 1 | 216 | 1 | 215 | 5.32e-96 | 279 |
| MS.gene065581.t1 | AT1G78360 | 61.395 | 215 | 82 | 1 | 1 | 214 | 1 | 215 | 2.00e-95 | 278 |
| MS.gene065581.t1 | AT1G78340 | 60.563 | 213 | 84 | 0 | 1 | 213 | 1 | 213 | 2.16e-95 | 277 |
| MS.gene065581.t1 | AT1G78320 | 61.951 | 205 | 78 | 0 | 1 | 205 | 1 | 205 | 1.94e-93 | 273 |
| MS.gene065581.t1 | AT1G17190 | 57.209 | 215 | 91 | 1 | 1 | 214 | 1 | 215 | 1.15e-88 | 260 |
| MS.gene065581.t1 | AT1G53680 | 54.673 | 214 | 95 | 2 | 3 | 214 | 6 | 219 | 4.97e-87 | 256 |
| MS.gene065581.t1 | AT3G43800 | 51.643 | 213 | 99 | 3 | 4 | 212 | 5 | 217 | 7.64e-78 | 233 |
| MS.gene065581.t1 | AT1G78320 | 53.659 | 205 | 68 | 1 | 1 | 205 | 1 | 178 | 2.06e-74 | 223 |
| MS.gene065581.t1 | AT1G10360 | 41.935 | 217 | 117 | 5 | 1 | 208 | 1 | 217 | 7.16e-50 | 162 |
| MS.gene065581.t1 | AT3G09270 | 42.647 | 204 | 114 | 2 | 5 | 205 | 7 | 210 | 8.20e-49 | 159 |
| MS.gene065581.t1 | AT1G59700 | 41.284 | 218 | 113 | 5 | 4 | 209 | 6 | 220 | 1.66e-46 | 154 |
| MS.gene065581.t1 | AT1G59670 | 44.495 | 218 | 106 | 5 | 4 | 209 | 6 | 220 | 5.83e-46 | 152 |
| MS.gene065581.t1 | AT2G29420 | 39.171 | 217 | 129 | 2 | 2 | 216 | 7 | 222 | 6.59e-46 | 152 |
| MS.gene065581.t1 | AT1G10370 | 39.189 | 222 | 121 | 4 | 2 | 212 | 3 | 221 | 6.24e-44 | 147 |
| MS.gene065581.t1 | AT1G74590 | 40.513 | 195 | 110 | 4 | 3 | 191 | 6 | 200 | 1.03e-42 | 144 |
| MS.gene065581.t1 | AT2G29490 | 37.674 | 215 | 130 | 2 | 5 | 216 | 8 | 221 | 2.41e-42 | 142 |
| MS.gene065581.t1 | AT1G27130 | 36.697 | 218 | 123 | 4 | 3 | 208 | 5 | 219 | 3.50e-42 | 142 |
| MS.gene065581.t1 | AT2G29470 | 37.089 | 213 | 127 | 5 | 5 | 212 | 8 | 218 | 1.83e-40 | 138 |
| MS.gene065581.t1 | AT1G27140 | 39.024 | 205 | 115 | 5 | 15 | 209 | 17 | 221 | 2.52e-40 | 138 |
| MS.gene065581.t1 | AT2G29480 | 40.488 | 205 | 114 | 6 | 5 | 204 | 8 | 209 | 8.63e-40 | 136 |
| MS.gene065581.t1 | AT2G29440 | 38.462 | 221 | 122 | 6 | 4 | 216 | 6 | 220 | 1.92e-39 | 135 |
| MS.gene065581.t1 | AT2G29450 | 39.234 | 209 | 114 | 6 | 4 | 204 | 6 | 209 | 2.55e-39 | 135 |
| MS.gene065581.t1 | AT2G29460 | 37.156 | 218 | 129 | 6 | 4 | 216 | 7 | 221 | 1.23e-38 | 133 |
| MS.gene065581.t1 | AT1G69920 | 35.874 | 223 | 114 | 5 | 5 | 208 | 35 | 247 | 4.11e-38 | 132 |
| MS.gene065581.t1 | AT1G69930 | 36.019 | 211 | 128 | 3 | 5 | 208 | 13 | 223 | 6.06e-38 | 132 |
| MS.gene065581.t1 | AT5G62480 | 33.036 | 224 | 140 | 3 | 1 | 214 | 5 | 228 | 1.69e-37 | 130 |
| MS.gene065581.t1 | AT5G62480 | 27.679 | 224 | 126 | 4 | 1 | 214 | 5 | 202 | 1.29e-21 | 89.0 |
| MS.gene065581.t1 | AT5G62480 | 27.679 | 224 | 125 | 4 | 1 | 214 | 5 | 201 | 1.51e-21 | 89.0 |
Find 36 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGAGCTCAAGCTAGATTT+TGG | 0.158333 | 2.2:-21903823 | MS.gene065581:CDS |
| GCATAAAACCAGCTATAAAA+AGG | 0.227426 | 2.2:+21902908 | None:intergenic |
| ACTGGAATCTTCTTATGAAT+TGG | 0.280289 | 2.2:+21903950 | None:intergenic |
| TGCAATACATAGATGAAGTT+TGG | 0.296178 | 2.2:-21903883 | MS.gene065581:CDS |
| ACGTTTGGGTTTCTTGATAT+TGG | 0.309792 | 2.2:-21902938 | MS.gene065581:CDS |
| GGTTGATTATAGTGACAAAA+AGG | 0.317820 | 2.2:-21903801 | MS.gene065581:intron |
| GACATATGGCAACTTCAAAA+TGG | 0.325407 | 2.2:-21902879 | MS.gene065581:CDS |
| ATTCCAGTTCTTATCCATAA+TGG | 0.326052 | 2.2:-21903935 | MS.gene065581:CDS |
| GATACATGGGCTAGCATGTT+TGG | 0.368138 | 2.2:-21904070 | MS.gene065581:CDS |
| TTGGTCCAAGCCATTAGTTT+AGG | 0.371833 | 2.2:+21902845 | None:intergenic |
| TTTATGGTGGTGACACGTTT+GGG | 0.375918 | 2.2:-21902952 | MS.gene065581:CDS |
| TTTCCATTATGGATAAGAAC+TGG | 0.399668 | 2.2:+21903932 | None:intergenic |
| ATCTCTCTCCATGCATCTCT+TGG | 0.408511 | 2.2:+21902826 | None:intergenic |
| GCTCTCTCATAAGGATTAGA+AGG | 0.410231 | 2.2:+21903839 | None:intergenic |
| TCATACACCTTCTTCTGATC+AGG | 0.419283 | 2.2:+21902785 | None:intergenic |
| AAGAAAATGTGGCTTTCAAA+AGG | 0.435917 | 2.2:-21903052 | MS.gene065581:CDS |
| AGAATATGAGGAAGGGAAGA+TGG | 0.442414 | 2.2:-21903026 | MS.gene065581:CDS |
| AACGGAGTGTCCTAAACTAA+TGG | 0.448765 | 2.2:-21902855 | MS.gene065581:CDS |
| CTAGCTTGAGCTCTCTCATA+AGG | 0.462092 | 2.2:+21903830 | None:intergenic |
| AATAATTGCAGATTCACAAA+TGG | 0.471536 | 2.2:+21903907 | None:intergenic |
| AAAGGAGAAGAATATGAGGA+AGG | 0.489666 | 2.2:-21903034 | MS.gene065581:CDS |
| ATCTCTTCCTGATCAGAAGA+AGG | 0.501564 | 2.2:-21902792 | MS.gene065581:CDS |
| TTCAAAAGGAGAAGAATATG+AGG | 0.519962 | 2.2:-21903038 | MS.gene065581:CDS |
| TGGCAACTTCAAAATGGAAA+CGG | 0.530941 | 2.2:-21902873 | MS.gene065581:CDS |
| ATGACACTTGGAAGAAAATG+TGG | 0.537717 | 2.2:-21903063 | MS.gene065581:CDS |
| AGGTAATTCTGTTAGATACA+TGG | 0.538022 | 2.2:-21904084 | MS.gene065581:CDS |
| AAGGAGAAGAATATGAGGAA+GGG | 0.539682 | 2.2:-21903033 | MS.gene065581:CDS |
| ATATGTAGGTTTATGACACT+TGG | 0.541669 | 2.2:-21903075 | MS.gene065581:intron |
| AGTGTCCTAAACTAATGGCT+TGG | 0.548948 | 2.2:-21902850 | MS.gene065581:CDS |
| TCTATCTTTAAGACACTAGA+GGG | 0.555267 | 2.2:-21902995 | MS.gene065581:CDS |
| GGATTCATCTGAAGAAGTAA+TGG | 0.570292 | 2.2:+21903971 | None:intergenic |
| GGTAATTCTGTTAGATACAT+GGG | 0.584405 | 2.2:-21904083 | MS.gene065581:CDS |
| CTCTATCTTTAAGACACTAG+AGG | 0.601881 | 2.2:-21902996 | MS.gene065581:CDS |
| GGCTTGGACCAAGAGATGCA+TGG | 0.654997 | 2.2:-21902834 | MS.gene065581:CDS |
| CTATCTTTAAGACACTAGAG+GGG | 0.684854 | 2.2:-21902994 | MS.gene065581:CDS |
| TATCTTTAAGACACTAGAGG+GGG | 0.687022 | 2.2:-21902993 | MS.gene065581:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAATAAATAAGTATATTGA+GGG | + | chr2.2:21903366-21903385 | None:intergenic | 10.0% |
| !! | AAATTGATTATGAAATTAAG+AGG | + | chr2.2:21903097-21903116 | None:intergenic | 15.0% |
| !! | GATAATAAATAAGTATATTG+AGG | + | chr2.2:21903367-21903386 | None:intergenic | 15.0% |
| !!! | CTTAATTTCATAATCAATTT+TGG | - | chr2.2:21903097-21903116 | MS.gene065581:intron | 15.0% |
| !! | AAAAAGGTAACTTCTTAATT+TGG | - | chr2.2:21903063-21903082 | MS.gene065581:CDS | 20.0% |
| !! | AAAAGGTAACTTCTTAATTT+GGG | - | chr2.2:21903064-21903083 | MS.gene065581:CDS | 20.0% |
| !! | ACATTAACTCTATTCATATA+AGG | + | chr2.2:21903425-21903444 | None:intergenic | 20.0% |
| !! | GTTCATTCAAAAATTAAACT+CGG | - | chr2.2:21903212-21903231 | MS.gene065581:intron | 20.0% |
| !!! | AATTTCATAATCAATTTTGG+TGG | - | chr2.2:21903100-21903119 | MS.gene065581:intron | 20.0% |
| !!! | ATTTCATAATCAATTTTGGT+GGG | - | chr2.2:21903101-21903120 | MS.gene065581:intron | 20.0% |
| !!! | GACTTTTTAAATATAACTCA+AGG | + | chr2.2:21903243-21903262 | None:intergenic | 20.0% |
| !!! | TTTAATGTTTGAGACTATTT+TGG | - | chr2.2:21903462-21903481 | MS.gene065581:intron | 20.0% |
| !!! | TTTTTAAATATAACTCAAGG+AGG | + | chr2.2:21903240-21903259 | None:intergenic | 20.0% |
| ! | AATAATTGCAGATTCACAAA+TGG | + | chr2.2:21902944-21902963 | None:intergenic | 25.0% |
| ! | CATAAAACCAGCTATAAAAA+GGG | + | chr2.2:21903942-21903961 | None:intergenic | 25.0% |
| ! | TTGATCTTGAATGTATATGT+AGG | - | chr2.2:21903759-21903778 | MS.gene065581:intron | 25.0% |
| !! | ATTGTCCTTCGTTTAATTTA+GGG | + | chr2.2:21903725-21903744 | None:intergenic | 25.0% |
| !! | ATTTTGAATTTAACCCACTT+GGG | - | chr2.2:21903510-21903529 | MS.gene065581:intron | 25.0% |
| !! | TATTGTCCTTCGTTTAATTT+AGG | + | chr2.2:21903726-21903745 | None:intergenic | 25.0% |
| !!! | TATTTTGAATTTAACCCACT+TGG | - | chr2.2:21903509-21903528 | MS.gene065581:intron | 25.0% |
| !!! | TTTTATGCTTTTGAGACATA+TGG | - | chr2.2:21903955-21903974 | MS.gene065581:CDS | 25.0% |
| AAGAAAATGTGGCTTTCAAA+AGG | - | chr2.2:21903796-21903815 | MS.gene065581:intron | 30.0% | |
| ACTGGAATCTTCTTATGAAT+TGG | + | chr2.2:21902901-21902920 | None:intergenic | 30.0% | |
| AGGTAATTCTGTTAGATACA+TGG | - | chr2.2:21902764-21902783 | MS.gene065581:CDS | 30.0% | |
| ATATGTAGGTTTATGACACT+TGG | - | chr2.2:21903773-21903792 | MS.gene065581:intron | 30.0% | |
| ATTACCCCTAAATTAAACGA+AGG | - | chr2.2:21903717-21903736 | MS.gene065581:intron | 30.0% | |
| ATTCCAGTTCTTATCCATAA+TGG | - | chr2.2:21902913-21902932 | MS.gene065581:CDS | 30.0% | |
| CGAAGGACAATACAAATATT+CGG | - | chr2.2:21903734-21903753 | MS.gene065581:intron | 30.0% | |
| GCATAAAACCAGCTATAAAA+AGG | + | chr2.2:21903943-21903962 | None:intergenic | 30.0% | |
| GGTAATTCTGTTAGATACAT+GGG | - | chr2.2:21902765-21902784 | MS.gene065581:CDS | 30.0% | |
| GGTTGATTATAGTGACAAAA+AGG | - | chr2.2:21903047-21903066 | MS.gene065581:CDS | 30.0% | |
| TGCAATACATAGATGAAGTT+TGG | - | chr2.2:21902965-21902984 | MS.gene065581:CDS | 30.0% | |
| TTCAAAAGGAGAAGAATATG+AGG | - | chr2.2:21903810-21903829 | MS.gene065581:CDS | 30.0% | |
| TTTCCATTATGGATAAGAAC+TGG | + | chr2.2:21902919-21902938 | None:intergenic | 30.0% | |
| ! | TTGTCCTTCGTTTAATTTAG+GGG | + | chr2.2:21903724-21903743 | None:intergenic | 30.0% |
| !! | CACAAATGGATTTTCCATTA+TGG | + | chr2.2:21902930-21902949 | None:intergenic | 30.0% |
| !! | TCTATCTTTAAGACACTAGA+GGG | - | chr2.2:21903853-21903872 | MS.gene065581:CDS | 30.0% |
| !!! | TTGAGTGACAAGCTTTTTTA+TGG | - | chr2.2:21903880-21903899 | MS.gene065581:CDS | 30.0% |
| AAAGGAGAAGAATATGAGGA+AGG | - | chr2.2:21903814-21903833 | MS.gene065581:CDS | 35.0% | |
| AAGGAGAAGAATATGAGGAA+GGG | - | chr2.2:21903815-21903834 | MS.gene065581:CDS | 35.0% | |
| ATGACACTTGGAAGAAAATG+TGG | - | chr2.2:21903785-21903804 | MS.gene065581:intron | 35.0% | |
| GACATATGGCAACTTCAAAA+TGG | - | chr2.2:21903969-21903988 | MS.gene065581:CDS | 35.0% | |
| GGATTCATCTGAAGAAGTAA+TGG | + | chr2.2:21902880-21902899 | None:intergenic | 35.0% | |
| TGGCAACTTCAAAATGGAAA+CGG | - | chr2.2:21903975-21903994 | MS.gene065581:CDS | 35.0% | |
| ! | ACGTTTGGGTTTCTTGATAT+TGG | - | chr2.2:21903910-21903929 | MS.gene065581:CDS | 35.0% |
| ! | GTTTGATCCCTTTTTATAGC+TGG | - | chr2.2:21903932-21903951 | MS.gene065581:CDS | 35.0% |
| ! | TAGAGTCTTGTCTCATTGAA+GGG | + | chr2.2:21903142-21903161 | None:intergenic | 35.0% |
| ! | TTAGAGTCTTGTCTCATTGA+AGG | + | chr2.2:21903143-21903162 | None:intergenic | 35.0% |
| !! | CTATCTTTAAGACACTAGAG+GGG | - | chr2.2:21903854-21903873 | MS.gene065581:CDS | 35.0% |
| !! | CTCTATCTTTAAGACACTAG+AGG | - | chr2.2:21903852-21903871 | MS.gene065581:CDS | 35.0% |
| !! | TATCTTTAAGACACTAGAGG+GGG | - | chr2.2:21903855-21903874 | MS.gene065581:CDS | 35.0% |
| !!! | AGTGACAAGCTTTTTTATGG+TGG | - | chr2.2:21903883-21903902 | MS.gene065581:CDS | 35.0% |
| AGAATATGAGGAAGGGAAGA+TGG | - | chr2.2:21903822-21903841 | MS.gene065581:CDS | 40.0% | |
| AGTGTCCTAAACTAATGGCT+TGG | - | chr2.2:21903998-21904017 | MS.gene065581:CDS | 40.0% | |
| ATCTCTTCCTGATCAGAAGA+AGG | - | chr2.2:21904056-21904075 | MS.gene065581:CDS | 40.0% | |
| CTAAACTAGCCCACCTAAAT+TGG | + | chr2.2:21903610-21903629 | None:intergenic | 40.0% | |
| GCTCTCTCATAAGGATTAGA+AGG | + | chr2.2:21903012-21903031 | None:intergenic | 40.0% | |
| TCATACACCTTCTTCTGATC+AGG | + | chr2.2:21904066-21904085 | None:intergenic | 40.0% | |
| ! | AACGGAGTGTCCTAAACTAA+TGG | - | chr2.2:21903993-21904012 | MS.gene065581:CDS | 40.0% |
| ! | AGAGAGCTCAAGCTAGATTT+TGG | - | chr2.2:21903025-21903044 | MS.gene065581:CDS | 40.0% |
| ! | ATTCTCCATGGTGCCAATTT+AGG | - | chr2.2:21903594-21903613 | MS.gene065581:intron | 40.0% |
| ! | GAGAGCTCAAGCTAGATTTT+GGG | - | chr2.2:21903026-21903045 | MS.gene065581:CDS | 40.0% |
| ! | TTGGTCCAAGCCATTAGTTT+AGG | + | chr2.2:21904006-21904025 | None:intergenic | 40.0% |
| ! | TTTATGGTGGTGACACGTTT+GGG | - | chr2.2:21903896-21903915 | MS.gene065581:CDS | 40.0% |
| !! | TTTTATGGTGGTGACACGTT+TGG | - | chr2.2:21903895-21903914 | MS.gene065581:CDS | 40.0% |
| ATCTCTCTCCATGCATCTCT+TGG | + | chr2.2:21904025-21904044 | None:intergenic | 45.0% | |
| CTAGCTTGAGCTCTCTCATA+AGG | + | chr2.2:21903021-21903040 | None:intergenic | 45.0% | |
| ! | TCCATGGTGCCAATTTAGGT+GGG | - | chr2.2:21903598-21903617 | MS.gene065581:intron | 45.0% |
| !! | GATACATGGGCTAGCATGTT+TGG | - | chr2.2:21902778-21902797 | MS.gene065581:CDS | 45.0% |
| !! | GTGGTATTGGTTTGGGATCT+GGG | - | chr2.2:21903541-21903560 | MS.gene065581:intron | 45.0% |
| !! | TGACCTGGTGGTATTGGTTT+GGG | - | chr2.2:21903534-21903553 | MS.gene065581:intron | 45.0% |
| !! | TTGACCTGGTGGTATTGGTT+TGG | - | chr2.2:21903533-21903552 | MS.gene065581:intron | 45.0% |
| GATCCCAAACCAATACCACC+AGG | + | chr2.2:21903540-21903559 | None:intergenic | 50.0% | |
| GTCTCAGGTTCGATTCTCCA+TGG | - | chr2.2:21903582-21903601 | MS.gene065581:intron | 50.0% | |
| ! | CTCCATGGTGCCAATTTAGG+TGG | - | chr2.2:21903597-21903616 | MS.gene065581:intron | 50.0% |
| !! | GGTGGTATTGGTTTGGGATC+TGG | - | chr2.2:21903540-21903559 | MS.gene065581:intron | 50.0% |
| !! | TTAACCCACTTGGGTTGACC+TGG | - | chr2.2:21903519-21903538 | MS.gene065581:intron | 50.0% |
| !! | TTGGGTTGACCTGGTGGTAT+TGG | - | chr2.2:21903528-21903547 | MS.gene065581:intron | 50.0% |
| ACCACCAGGTCAACCCAAGT+GGG | + | chr2.2:21903526-21903545 | None:intergenic | 55.0% | |
| GAATCGAACCTGAGACCTCG+AGG | + | chr2.2:21903578-21903597 | None:intergenic | 55.0% | |
| GCCCACCTAAATTGGCACCA+TGG | + | chr2.2:21903602-21903621 | None:intergenic | 55.0% | |
| GGCTTGGACCAAGAGATGCA+TGG | - | chr2.2:21904014-21904033 | MS.gene065581:CDS | 55.0% | |
| GTGCTTATCCTCGAGGTCTC+AGG | - | chr2.2:21903567-21903586 | MS.gene065581:intron | 55.0% | |
| TACCACCAGGTCAACCCAAG+TGG | + | chr2.2:21903527-21903546 | None:intergenic | 55.0% | |
| ! | TGGGAGTGTGCTTATCCTCG+AGG | - | chr2.2:21903560-21903579 | MS.gene065581:intron | 55.0% |
| !! | ACCCACTTGGGTTGACCTGG+TGG | - | chr2.2:21903522-21903541 | MS.gene065581:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.2 | gene | 21902754 | 21904116 | 21902754 | ID=MS.gene065581 |
| chr2.2 | mRNA | 21902754 | 21904116 | 21902754 | ID=MS.gene065581.t1;Parent=MS.gene065581 |
| chr2.2 | exon | 21903802 | 21904116 | 21903802 | ID=MS.gene065581.t1.exon1;Parent=MS.gene065581.t1 |
| chr2.2 | CDS | 21903802 | 21904116 | 21903802 | ID=cds.MS.gene065581.t1;Parent=MS.gene065581.t1 |
| chr2.2 | exon | 21902754 | 21903089 | 21902754 | ID=MS.gene065581.t1.exon2;Parent=MS.gene065581.t1 |
| chr2.2 | CDS | 21902754 | 21903089 | 21902754 | ID=cds.MS.gene065581.t1;Parent=MS.gene065581.t1 |
| Gene Sequence |
| Protein sequence |
