AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066427


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066427.t1 MTR_8g030830 96.066 305 12 0 1 305 1 305 0.0 583
MS.gene066427.t1 MTR_8g446960 96.066 305 12 0 1 305 1 305 0.0 562
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066427.t1 AT1G13870 70.627 303 84 4 1 303 1 298 6.99e-157 440

Find 60 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATTCTTTGAATAACATTA+AGG 0.212951 8.1:+72092780 MS.gene066427:CDS
CGATGCAATATTTGAAGATT+TGG 0.217452 8.1:+72092936 MS.gene066427:CDS
AGAATTTAAGAGGAGTTCTT+AGG 0.298956 8.1:+72092713 MS.gene066427:CDS
CCAGCTAATGGCATTTCTAT+AGG 0.311339 8.1:+72093252 MS.gene066427:CDS
TGGTGTTTGGCTCGTGCTGC+AGG 0.319369 8.1:+72092820 MS.gene066427:CDS
TCCCATCTATTTCTTCTCTC+TGG 0.324920 8.1:-72092976 None:intergenic
CCGATATGAGCTATGGTGTT+TGG 0.374786 8.1:+72092807 MS.gene066427:CDS
TGACGATTCTTTGAGAGCTT+CGG 0.376709 8.1:-72092605 None:intergenic
GGCTAGACTTAGCGCAGCTT+TGG 0.378208 8.1:-72092584 None:intergenic
CAGCTAATGGCATTTCTATA+GGG 0.379509 8.1:+72093253 MS.gene066427:CDS
AGGATGATGGTTGTAGAAAA+TGG 0.390890 8.1:+72092875 MS.gene066427:CDS
GATTCTTTGAATAACATTAA+GGG 0.396934 8.1:+72092781 MS.gene066427:CDS
AACCAGAGAGAAGAAATAGA+TGG 0.404380 8.1:+72092974 MS.gene066427:CDS
CTTGGTATGCTACCAGCTAA+TGG 0.416633 8.1:+72093240 MS.gene066427:CDS
TACTGTGATGTTGAGGATGA+TGG 0.439873 8.1:+72092862 MS.gene066427:CDS
CGCACATTCATGAAATTGAC+AGG 0.447358 8.1:+72093339 MS.gene066427:CDS
GAAGCGCAATCTCGTGATCT+TGG 0.451998 8.1:+72093222 MS.gene066427:CDS
ATCAATCTTTCGAGATCAGT+TGG 0.454210 8.1:+72093291 MS.gene066427:CDS
GTGTTGCTTTATCCAGCTCA+TGG 0.454264 8.1:-72093176 None:intergenic
ACTGATCTCGAAAGATTGAT+AGG 0.457284 8.1:-72093288 None:intergenic
CCATCAGAGAAGAATTTAAG+AGG 0.458052 8.1:+72092703 MS.gene066427:CDS
GATTACTTGAACAGAGAACT+TGG 0.460253 8.1:+72093426 MS.gene066427:CDS
CGTCGCAACCTACGAAGCTC+TGG 0.462348 8.1:-72093318 None:intergenic
TGCAAATTCTCTCCATGAGC+TGG 0.472289 8.1:+72093164 MS.gene066427:CDS
GAAGTTTGAGTCGCAATAGT+TGG 0.474380 8.1:-72093129 None:intergenic
GAGACCTCCTCCTTCTGATT+CGG 0.480952 8.1:+72093380 MS.gene066427:CDS
TTCGTAGTTTCAAATGGCAC+TGG 0.481368 8.1:+72092528 None:intergenic
TTCTTAGGTCAGAAGTTGAC+AGG 0.485003 8.1:+72092728 MS.gene066427:CDS
CCTCTTAAATTCTTCTCTGA+TGG 0.488428 8.1:-72092703 None:intergenic
AAGCTTGTTTCCATCTTGAT+CGG 0.496584 8.1:+72092659 MS.gene066427:CDS
CAATATTTGAAGATTTGGTA+AGG 0.503933 8.1:+72092941 MS.gene066427:CDS
CCTATAGAAATGCCATTAGC+TGG 0.505528 8.1:-72093252 None:intergenic
TCAATCTTTCGAGATCAGTT+GGG 0.511829 8.1:+72093292 MS.gene066427:CDS
AACTTTGATTCCGATCAAGA+TGG 0.519631 8.1:-72092669 None:intergenic
TTTGTTTGAGTTGAAATCAT+CGG 0.521912 8.1:+72093005 MS.gene066427:CDS
CCAAACACCATAGCTCATAT+CGG 0.531120 8.1:-72092807 None:intergenic
TTGGGTTGCCAGAGCTTCGT+AGG 0.533470 8.1:+72093310 MS.gene066427:CDS
AATCAAGAGCGAAGGGAGAA+AGG 0.534973 8.1:+72092898 MS.gene066427:CDS
ACCAGAGAGAAGAAATAGAT+GGG 0.553983 8.1:+72092975 MS.gene066427:CDS
TGTTGTTTACTGTGATGTTG+AGG 0.554593 8.1:+72092855 MS.gene066427:CDS
TTGCAGTATCTTCACATCGC+GGG 0.554850 8.1:-72093106 None:intergenic
GGTGTTTGGCTCGTGCTGCA+GGG 0.562065 8.1:+72092821 MS.gene066427:CDS
GATGATTTCAACTCAAACAA+AGG 0.581112 8.1:-72093003 None:intergenic
TATTTGAAGATTTGGTAAGG+AGG 0.589953 8.1:+72092944 MS.gene066427:CDS
AGGGTTACCGATATGAGCTA+TGG 0.591762 8.1:+72092800 MS.gene066427:CDS
CAGGGCAAACAAGTTTGAGT+GGG 0.594880 8.1:+72093358 MS.gene066427:CDS
GAAAATGGAATCAAGAGCGA+AGG 0.596859 8.1:+72092890 MS.gene066427:CDS
ACAGGGCAAACAAGTTTGAG+TGG 0.597798 8.1:+72093357 MS.gene066427:CDS
TTAGCACTATCCGAATCAGA+AGG 0.613709 8.1:-72093390 None:intergenic
AAAATGGAATCAAGAGCGAA+GGG 0.624157 8.1:+72092891 MS.gene066427:CDS
GACAGCATCATCTACAACGG+AGG 0.629029 8.1:-72093052 None:intergenic
ATGGCACTGGTTGTAATATG+TGG 0.645253 8.1:+72092541 MS.gene066427:CDS
CTATCCGAATCAGAAGGAGG+AGG 0.657212 8.1:-72093384 None:intergenic
GATCTCGAAAGATTGATAGG+AGG 0.664316 8.1:-72093285 None:intergenic
GCACTATCCGAATCAGAAGG+AGG 0.671033 8.1:-72093387 None:intergenic
GCTGGATAAAGCAACACAAG+AGG 0.673279 8.1:+72093182 MS.gene066427:CDS
ATATGTGGTCAACCATGCAG+TGG 0.681649 8.1:+72092556 MS.gene066427:CDS
GTTGCAGTATCTTCACATCG+CGG 0.688808 8.1:-72093107 None:intergenic
AGAGACAGCATCATCTACAA+CGG 0.703655 8.1:-72093055 None:intergenic
GCACATTCATGAAATTGACA+GGG 0.710510 8.1:+72093340 MS.gene066427:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATTCTTTGAATAACATTAA+GGG + chr8.1:72092781-72092800 MS.gene066427:CDS 20.0%
!! TGATTCTTTGAATAACATTA+AGG + chr8.1:72092780-72092799 MS.gene066427:CDS 20.0%
!! CAATATTTGAAGATTTGGTA+AGG + chr8.1:72092941-72092960 MS.gene066427:CDS 25.0%
!!! TTTGTTTGAGTTGAAATCAT+CGG + chr8.1:72093005-72093024 MS.gene066427:CDS 25.0%
AGAATTTAAGAGGAGTTCTT+AGG + chr8.1:72092713-72092732 MS.gene066427:CDS 30.0%
CGATGCAATATTTGAAGATT+TGG + chr8.1:72092936-72092955 MS.gene066427:CDS 30.0%
CTCTTACATAACCAAAAAAG+TGG + chr8.1:72093074-72093093 MS.gene066427:CDS 30.0%
GATGATTTCAACTCAAACAA+AGG - chr8.1:72093006-72093025 None:intergenic 30.0%
! TATTTGAAGATTTGGTAAGG+AGG + chr8.1:72092944-72092963 MS.gene066427:CDS 30.0%
AAAATGGAATCAAGAGCGAA+GGG + chr8.1:72092891-72092910 MS.gene066427:CDS 35.0%
AACCAGAGAGAAGAAATAGA+TGG + chr8.1:72092974-72092993 MS.gene066427:CDS 35.0%
AACTTTGATTCCGATCAAGA+TGG - chr8.1:72092672-72092691 None:intergenic 35.0%
ACCAGAGAGAAGAAATAGAT+GGG + chr8.1:72092975-72092994 MS.gene066427:CDS 35.0%
ACTGATCTCGAAAGATTGAT+AGG - chr8.1:72093291-72093310 None:intergenic 35.0%
ATCAATCTTTCGAGATCAGT+TGG + chr8.1:72093291-72093310 MS.gene066427:CDS 35.0%
CAGCTAATGGCATTTCTATA+GGG + chr8.1:72093253-72093272 MS.gene066427:CDS 35.0%
CCATCAGAGAAGAATTTAAG+AGG + chr8.1:72092703-72092722 MS.gene066427:CDS 35.0%
CCTCTTAAATTCTTCTCTGA+TGG - chr8.1:72092706-72092725 None:intergenic 35.0%
GATTACTTGAACAGAGAACT+TGG + chr8.1:72093426-72093445 MS.gene066427:CDS 35.0%
GCACATTCATGAAATTGACA+GGG + chr8.1:72093340-72093359 MS.gene066427:CDS 35.0%
TCAATCTTTCGAGATCAGTT+GGG + chr8.1:72093292-72093311 MS.gene066427:CDS 35.0%
! AAGCTTGTTTCCATCTTGAT+CGG + chr8.1:72092659-72092678 MS.gene066427:CDS 35.0%
! AGGATGATGGTTGTAGAAAA+TGG + chr8.1:72092875-72092894 MS.gene066427:CDS 35.0%
! GACTCAAACTTCACGTTTTT+CGG + chr8.1:72093140-72093159 MS.gene066427:CDS 35.0%
! TGTTGTTTACTGTGATGTTG+AGG + chr8.1:72092855-72092874 MS.gene066427:CDS 35.0%
!!! GGTTTTTGAGTCCACTTTTT+TGG - chr8.1:72093088-72093107 None:intergenic 35.0%
AGAGACAGCATCATCTACAA+CGG - chr8.1:72093058-72093077 None:intergenic 40.0%
CCAAACACCATAGCTCATAT+CGG - chr8.1:72092810-72092829 None:intergenic 40.0%
CCAGCTAATGGCATTTCTAT+AGG + chr8.1:72093252-72093271 MS.gene066427:CDS 40.0%
CCTATAGAAATGCCATTAGC+TGG - chr8.1:72093255-72093274 None:intergenic 40.0%
CGCACATTCATGAAATTGAC+AGG + chr8.1:72093339-72093358 MS.gene066427:CDS 40.0%
GAAAATGGAATCAAGAGCGA+AGG + chr8.1:72092890-72092909 MS.gene066427:CDS 40.0%
GAAGTTTGAGTCGCAATAGT+TGG - chr8.1:72093132-72093151 None:intergenic 40.0%
GATCTCGAAAGATTGATAGG+AGG - chr8.1:72093288-72093307 None:intergenic 40.0%
TACTGTGATGTTGAGGATGA+TGG + chr8.1:72092862-72092881 MS.gene066427:CDS 40.0%
TTAGCACTATCCGAATCAGA+AGG - chr8.1:72093393-72093412 None:intergenic 40.0%
TTCTTAGGTCAGAAGTTGAC+AGG + chr8.1:72092728-72092747 MS.gene066427:CDS 40.0%
! TCCCATCTATTTCTTCTCTC+TGG - chr8.1:72092979-72092998 None:intergenic 40.0%
! TGACGATTCTTTGAGAGCTT+CGG - chr8.1:72092608-72092627 None:intergenic 40.0%
!! ATGGCACTGGTTGTAATATG+TGG + chr8.1:72092541-72092560 MS.gene066427:CDS 40.0%
AATCAAGAGCGAAGGGAGAA+AGG + chr8.1:72092898-72092917 MS.gene066427:CDS 45.0%
ACAGGGCAAACAAGTTTGAG+TGG + chr8.1:72093357-72093376 MS.gene066427:CDS 45.0%
AGGGTTACCGATATGAGCTA+TGG + chr8.1:72092800-72092819 MS.gene066427:CDS 45.0%
ATATGTGGTCAACCATGCAG+TGG + chr8.1:72092556-72092575 MS.gene066427:CDS 45.0%
CAGGGCAAACAAGTTTGAGT+GGG + chr8.1:72093358-72093377 MS.gene066427:CDS 45.0%
CCGATATGAGCTATGGTGTT+TGG + chr8.1:72092807-72092826 MS.gene066427:CDS 45.0%
CTTGGTATGCTACCAGCTAA+TGG + chr8.1:72093240-72093259 MS.gene066427:CDS 45.0%
GCTGGATAAAGCAACACAAG+AGG + chr8.1:72093182-72093201 MS.gene066427:CDS 45.0%
GTTGCAGTATCTTCACATCG+CGG - chr8.1:72093110-72093129 None:intergenic 45.0%
TGCAAATTCTCTCCATGAGC+TGG + chr8.1:72093164-72093183 MS.gene066427:CDS 45.0%
TTGCAGTATCTTCACATCGC+GGG - chr8.1:72093109-72093128 None:intergenic 45.0%
!! GCTTTGGATTTTCCACTGCA+TGG - chr8.1:72092571-72092590 None:intergenic 45.0%
!! GTGTTGCTTTATCCAGCTCA+TGG - chr8.1:72093179-72093198 None:intergenic 45.0%
CTATCCGAATCAGAAGGAGG+AGG - chr8.1:72093387-72093406 None:intergenic 50.0%
GAAGCGCAATCTCGTGATCT+TGG + chr8.1:72093222-72093241 MS.gene066427:CDS 50.0%
GACAGCATCATCTACAACGG+AGG - chr8.1:72093055-72093074 None:intergenic 50.0%
GAGACCTCCTCCTTCTGATT+CGG + chr8.1:72093380-72093399 MS.gene066427:CDS 50.0%
GCACTATCCGAATCAGAAGG+AGG - chr8.1:72093390-72093409 None:intergenic 50.0%
GGCTAGACTTAGCGCAGCTT+TGG - chr8.1:72092587-72092606 None:intergenic 55.0%
! TTGGGTTGCCAGAGCTTCGT+AGG + chr8.1:72093310-72093329 MS.gene066427:CDS 55.0%
CGTCGCAACCTACGAAGCTC+TGG - chr8.1:72093321-72093340 None:intergenic 60.0%
! GGTGTTTGGCTCGTGCTGCA+GGG + chr8.1:72092821-72092840 MS.gene066427:CDS 60.0%
! TGGTGTTTGGCTCGTGCTGC+AGG + chr8.1:72092820-72092839 MS.gene066427:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 72092541 72093458 72092541 ID=MS.gene066427
chr8.1 mRNA 72092541 72093458 72092541 ID=MS.gene066427.t1;Parent=MS.gene066427
chr8.1 exon 72092541 72093458 72092541 ID=MS.gene066427.t1.exon1;Parent=MS.gene066427.t1
chr8.1 CDS 72092541 72093458 72092541 ID=cds.MS.gene066427.t1;Parent=MS.gene066427.t1
Gene Sequence

>MS.gene066427

ATGGCACTGGTTGTAATATGTGGTCAACCATGCAGTGGAAAATCCAAAGCTGCGCTAAGTCTAGCCGAAGCTCTCAAAGAATCGTCAGAATTCAAACATCAAGTGAGAATCATAGATGAAGCTTGTTTCCATCTTGATCGGAATCAAAGTTATGCAAATATGCCATCAGAGAAGAATTTAAGAGGAGTTCTTAGGTCAGAAGTTGACAGGTCTCTGTCAAAAGATACTGTTATTATCGTTGATTCTTTGAATAACATTAAGGGTTACCGATATGAGCTATGGTGTTTGGCTCGTGCTGCAGGGATTAGATACTGTGTTGTTTACTGTGATGTTGAGGATGATGGTTGTAGAAAATGGAATCAAGAGCGAAGGGAGAAAGGTGAAGATAATTATGACGATGCAATATTTGAAGATTTGGTAAGGAGGTTTGAGAAACCAGAGAGAAGAAATAGATGGGATTCACCTTTGTTTGAGTTGAAATCATCGGCATCGTCTTCTTCGTCTGATTCTGCCTCCGTTGTAGATGATGCTGTCTCTTACATAACCAAAAAAGTGGACTCAAAAACCCGCGATGTGAAGATACTGCAACCAACTATTGCGACTCAAACTTCACGTTTTTCGGATGCAAATTCTCTCCATGAGCTGGATAAAGCAACACAAGAGGTTACTAATGCTATAGCAGAAGCGCAATCTCGTGATCTTGGTATGCTACCAGCTAATGGCATTTCTATAGGGAAAGACTTGCCTCCTATCAATCTTTCGAGATCAGTTGGGTTGCCAGAGCTTCGTAGGTTGCGACGCACATTCATGAAATTGACAGGGCAAACAAGTTTGAGTGGGAGACCTCCTCCTTCTGATTCGGATAGTGCTAAAAGAATGTTCATTGATTACTTGAACAGAGAACTTGGAACTTCATGA

Protein sequence

>MS.gene066427.t1

MALVVICGQPCSGKSKAALSLAEALKESSEFKHQVRIIDEACFHLDRNQSYANMPSEKNLRGVLRSEVDRSLSKDTVIIVDSLNNIKGYRYELWCLARAAGIRYCVVYCDVEDDGCRKWNQERREKGEDNYDDAIFEDLVRRFEKPERRNRWDSPLFELKSSASSSSSDSASVVDDAVSYITKKVDSKTRDVKILQPTIATQTSRFSDANSLHELDKATQEVTNAIAEAQSRDLGMLPANGISIGKDLPPINLSRSVGLPELRRLRRTFMKLTGQTSLSGRPPPSDSDSAKRMFIDYLNRELGTS