AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07777


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07777.t1 MTR_2g038250 97.059 102 3 0 69 170 143 244 8.23e-69 209
MS.gene07777.t1 MTR_1g022385 97.059 102 3 0 69 170 174 275 1.70e-68 209
MS.gene07777.t1 MTR_4g008160 88.235 102 12 0 69 170 142 243 2.09e-63 195
MS.gene07777.t1 MTR_1g022375 77.500 80 18 0 81 160 77 156 2.90e-40 133
MS.gene07777.t1 MTR_7g059170 54.902 102 46 0 69 170 145 246 2.37e-39 134
MS.gene07777.t1 MTR_1g108785 72.973 37 9 1 127 162 23 59 8.01e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07777.t1 AT2G44120 84.314 102 16 0 69 170 141 242 2.02e-62 192
MS.gene07777.t1 AT2G44120 84.314 102 16 0 69 170 146 247 2.54e-62 192
MS.gene07777.t1 AT2G01250 85.294 102 15 0 69 170 141 242 2.96e-62 192
MS.gene07777.t1 AT3G13580 83.333 102 17 0 69 170 143 244 2.58e-61 190
MS.gene07777.t1 AT3G13580 83.333 102 17 0 69 170 143 244 2.58e-61 190
MS.gene07777.t1 AT3G13580 83.333 102 17 0 69 170 143 244 2.58e-61 190
MS.gene07777.t1 AT3G13580 83.333 102 17 0 69 170 143 244 2.58e-61 190
MS.gene07777.t1 AT3G13580 83.333 102 17 0 69 170 143 244 2.58e-61 190
MS.gene07777.t1 AT3G13580 83.333 102 17 0 69 170 143 244 2.58e-61 190
MS.gene07777.t1 AT3G13580 83.333 102 17 0 69 170 143 244 2.58e-61 190
MS.gene07777.t1 AT3G13580 83.333 102 17 0 69 170 143 244 2.58e-61 190
MS.gene07777.t1 AT3G13580 83.333 102 17 0 69 170 143 244 2.58e-61 190
MS.gene07777.t1 AT1G80750 52.941 102 47 1 70 170 146 247 1.49e-29 108
MS.gene07777.t1 AT2G01250 72.973 37 10 0 69 105 141 177 9.94e-11 58.2

Find 38 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCATTAGAAGGATGAATT+AGG 0.270598 8.1:+80815034 MS.gene07777:CDS
TTTCAAGCTCAAAGCTCCTT+TGG 0.284641 8.1:+80814938 MS.gene07777:CDS
AGTGTTCAATTGCAGGTTCT+TGG 0.317764 8.1:+80814825 MS.gene07777:intron
TTCAAGCTCAAAGCTCCTTT+GGG 0.329811 8.1:+80814939 MS.gene07777:CDS
ATACTATTGTGCTTGGGATT+TGG 0.331008 8.1:+80813848 MS.gene07777:CDS
TTGATCTACAAGCGCGGTTA+TGG 0.337108 8.1:+80814476 MS.gene07777:CDS
TCAATTCTCTAACACTCTTC+AGG 0.373375 8.1:-80814457 None:intergenic
CTTTGTATACTATTGTGCTT+GGG 0.384420 8.1:+80813842 MS.gene07777:CDS
AAGGTGGTGATGCTGGTAAC+AGG 0.401808 8.1:+80814994 MS.gene07777:CDS
GTGTTCAATTGCAGGTTCTT+GGG 0.403327 8.1:+80814826 MS.gene07777:intron
GCTTTGTATACTATTGTGCT+TGG 0.452410 8.1:+80813841 MS.gene07777:CDS
ACATCAATGAACTCATTAGA+AGG 0.462677 8.1:+80815024 MS.gene07777:CDS
GAAGCGTGAAGCTAAGCTTA+AGG 0.464202 8.1:+80814417 MS.gene07777:intron
AAGCGTGAAGCTAAGCTTAA+GGG 0.467088 8.1:+80814418 MS.gene07777:intron
AGATAACTCTATCATTGAAC+AGG 0.482014 8.1:+80814529 MS.gene07777:CDS
AAGATAAATCACTATGTTGA+AGG 0.485048 8.1:+80814975 MS.gene07777:CDS
AAAGGAGCTTTGAGCTTGAA+AGG 0.504831 8.1:-80814936 None:intergenic
GAGAGGCAAACAACTTCCTA+TGG 0.504947 8.1:+80814913 MS.gene07777:CDS
ATAAGCTAGAGCAGCAGCCA+AGG 0.523738 8.1:+80813871 MS.gene07777:CDS
TATGTTGAAGGTGGTGATGC+TGG 0.533299 8.1:+80814987 MS.gene07777:CDS
AAGCTGAGAAAGAACATGGT+TGG 0.534698 8.1:+80814091 MS.gene07777:CDS
TTGCAGGTTCTTGGGAAACA+TGG 0.547799 8.1:+80814834 MS.gene07777:intron
ATCCACGAGATCATGACTGT+TGG 0.549865 8.1:+80814879 MS.gene07777:CDS
GCGGTTATGGAAAGGTTGAT+AGG 0.549876 8.1:+80814489 MS.gene07777:CDS
CTTTGAGCTTGAAAGGCCAT+AGG 0.568033 8.1:-80814929 None:intergenic
AAGCTCAAAGCTCCTTTGGG+TGG 0.570830 8.1:+80814942 MS.gene07777:CDS
TTCTTCTTCATGCCACCCAA+AGG 0.574976 8.1:-80814954 None:intergenic
ATCCAACAGTCATGATCTCG+TGG 0.585385 8.1:-80814881 None:intergenic
CTACAAGCGCGGTTATGGAA+AGG 0.599869 8.1:+80814481 MS.gene07777:CDS
CGTGAAGCTAAGCTTAAGGG+TGG 0.634387 8.1:+80814421 MS.gene07777:CDS
ATAAATCACTATGTTGAAGG+TGG 0.634554 8.1:+80814978 MS.gene07777:CDS
GAGAAAGCTGAGAAAGAACA+TGG 0.636998 8.1:+80814087 MS.gene07777:CDS
GAGGTCAGGAGAGGCTACAG+AGG 0.640976 8.1:+80814050 MS.gene07777:intron
AGGTGGTGATGCTGGTAACA+GGG 0.651466 8.1:+80814995 MS.gene07777:CDS
GCATCTACGGAGGTCAGGAG+AGG 0.664972 8.1:+80814041 MS.gene07777:intron
AGAGAATTGATCTACAAGCG+CGG 0.721136 8.1:+80814470 MS.gene07777:CDS
TGTTGGATCTCACTTCAGAG+AGG 0.724978 8.1:+80814896 MS.gene07777:CDS
TGGTGATGCTGGTAACAGGG+AGG 0.745630 8.1:+80814998 MS.gene07777:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAAATAGAAAAATTTAAC+CGG - chr8.1:80814796-80814815 None:intergenic 15.0%
!!! AATGTTTTAGAATTTTAGAA+TGG + chr8.1:80814355-80814374 MS.gene07777:intron 15.0%
!!! AGTTTGAATTAACTCAATTT+TGG + chr8.1:80813904-80813923 MS.gene07777:intron 20.0%
!!! TTAGAATATTTTCTTTCATG+TGG + chr8.1:80813930-80813949 MS.gene07777:intron 20.0%
!!! TTCTTTCTTTGACTTATATA+TGG + chr8.1:80814205-80814224 MS.gene07777:intron 20.0%
!!! TTTAGAATTTTAGAATGGTA+AGG + chr8.1:80814360-80814379 MS.gene07777:intron 20.0%
! AAGATAAATCACTATGTTGA+AGG + chr8.1:80814975-80814994 MS.gene07777:CDS 25.0%
! ACTATCATATAATAGTGACA+TGG + chr8.1:80814013-80814032 MS.gene07777:intron 25.0%
! CTTTCATATTTGTTCTTGTA+TGG + chr8.1:80814240-80814259 MS.gene07777:intron 25.0%
! GTTTATCCAATTAATAGTGA+GGG + chr8.1:80814312-80814331 MS.gene07777:intron 25.0%
! TCAAACTTAAAATACAACCT+TGG - chr8.1:80813891-80813910 None:intergenic 25.0%
! TGTTTATCCAATTAATAGTG+AGG + chr8.1:80814311-80814330 MS.gene07777:intron 25.0%
!! AATATTTTCTTTCATGTGGA+AGG + chr8.1:80813934-80813953 MS.gene07777:intron 25.0%
!! AATTTAAAGTACGTACTTGT+GGG + chr8.1:80814272-80814291 MS.gene07777:intron 25.0%
!! TAATTTAAAGTACGTACTTG+TGG + chr8.1:80814271-80814290 MS.gene07777:intron 25.0%
ACATCAATGAACTCATTAGA+AGG + chr8.1:80815024-80815043 MS.gene07777:CDS 30.0%
AGATAACTCTATCATTGAAC+AGG + chr8.1:80814529-80814548 MS.gene07777:CDS 30.0%
AGTCAAAGAAAGAATCTCAT+TGG - chr8.1:80814199-80814218 None:intergenic 30.0%
ATAAATCACTATGTTGAAGG+TGG + chr8.1:80814978-80814997 MS.gene07777:CDS 30.0%
CTTTGTATACTATTGTGCTT+GGG + chr8.1:80813842-80813861 MS.gene07777:CDS 30.0%
!! GGTGCTATTATTATTGTTGA+AGG + chr8.1:80814762-80814781 MS.gene07777:intron 30.0%
AACAACTCACATTCTGACTT+TGG - chr8.1:80814667-80814686 None:intergenic 35.0%
AATAATAGCACCACCATACA+GGG - chr8.1:80814754-80814773 None:intergenic 35.0%
AATTGGGTAAAGTACAGAGT+CGG - chr8.1:80814642-80814661 None:intergenic 35.0%
ACATTCTGACTTTGGCAAAT+TGG - chr8.1:80814659-80814678 None:intergenic 35.0%
ATACATGCTAGCATTCTAGT+AGG + chr8.1:80813961-80813980 MS.gene07777:intron 35.0%
ATACTATTGTGCTTGGGATT+TGG + chr8.1:80813848-80813867 MS.gene07777:CDS 35.0%
ATCCAATTAATAGTGAGGGA+TGG + chr8.1:80814316-80814335 MS.gene07777:intron 35.0%
ATTGTTGAAGGTTATGTTGC+CGG + chr8.1:80814774-80814793 MS.gene07777:intron 35.0%
CATTCTGACTTTGGCAAATT+GGG - chr8.1:80814658-80814677 None:intergenic 35.0%
CTGAACTGTATGACATAGAT+GGG + chr8.1:80814603-80814622 MS.gene07777:intron 35.0%
GCTTTGTATACTATTGTGCT+TGG + chr8.1:80813841-80813860 MS.gene07777:CDS 35.0%
GTGATAACTACATCCAATGA+GGG - chr8.1:80814172-80814191 None:intergenic 35.0%
TAATAATAGCACCACCATAC+AGG - chr8.1:80814755-80814774 None:intergenic 35.0%
TACCATCCCTCACTATTAAT+TGG - chr8.1:80814321-80814340 None:intergenic 35.0%
TCAATTCTCTAACACTCTTC+AGG - chr8.1:80814460-80814479 None:intergenic 35.0%
TCTGAACTGTATGACATAGA+TGG + chr8.1:80814602-80814621 MS.gene07777:intron 35.0%
TGACATGGTTTATGCATCTA+CGG + chr8.1:80814028-80814047 MS.gene07777:intron 35.0%
TGTGATAACTACATCCAATG+AGG - chr8.1:80814173-80814192 None:intergenic 35.0%
!!! CTTTGAAACATCCTTTTCCA+GGG + chr8.1:80814143-80814162 MS.gene07777:intron 35.0%
!!! TCTTTGAAACATCCTTTTCC+AGG + chr8.1:80814142-80814161 MS.gene07777:intron 35.0%
AAGCGTGAAGCTAAGCTTAA+GGG + chr8.1:80814418-80814437 MS.gene07777:intron 40.0%
AAGCTGAGAAAGAACATGGT+TGG + chr8.1:80814091-80814110 MS.gene07777:CDS 40.0%
AGAGAATTGATCTACAAGCG+CGG + chr8.1:80814470-80814489 MS.gene07777:CDS 40.0%
AGTGTTCAATTGCAGGTTCT+TGG + chr8.1:80814825-80814844 MS.gene07777:intron 40.0%
ATAATAGCACCACCATACAG+GGG - chr8.1:80814753-80814772 None:intergenic 40.0%
CTGACATAGTGTTCAATTGC+AGG + chr8.1:80814818-80814837 MS.gene07777:intron 40.0%
GAGAAAGCTGAGAAAGAACA+TGG + chr8.1:80814087-80814106 MS.gene07777:CDS 40.0%
GGAATACGATGATTCGCAAA+AGG + chr8.1:80814381-80814400 MS.gene07777:intron 40.0%
GTGTTCAATTGCAGGTTCTT+GGG + chr8.1:80814826-80814845 MS.gene07777:intron 40.0%
TGTGTGATCGAATGAAAGCA+GGG - chr8.1:80814574-80814593 None:intergenic 40.0%
TTCAAGCTCAAAGCTCCTTT+GGG + chr8.1:80814939-80814958 MS.gene07777:CDS 40.0%
TTTCAAGCTCAAAGCTCCTT+TGG + chr8.1:80814938-80814957 MS.gene07777:CDS 40.0%
!! AAAGGAGCTTTGAGCTTGAA+AGG - chr8.1:80814939-80814958 None:intergenic 40.0%
AATGAGGGATTCCCTGGAAA+AGG - chr8.1:80814157-80814176 None:intergenic 45.0%
ATCCAACAGTCATGATCTCG+TGG - chr8.1:80814884-80814903 None:intergenic 45.0%
ATCCACGAGATCATGACTGT+TGG + chr8.1:80814879-80814898 MS.gene07777:CDS 45.0%
CATGGTTTATGCATCTACGG+AGG + chr8.1:80814031-80814050 MS.gene07777:intron 45.0%
CTGTGTGATCGAATGAAAGC+AGG - chr8.1:80814575-80814594 None:intergenic 45.0%
CTTTGAGCTTGAAAGGCCAT+AGG - chr8.1:80814932-80814951 None:intergenic 45.0%
GAAGCGTGAAGCTAAGCTTA+AGG + chr8.1:80814417-80814436 MS.gene07777:intron 45.0%
GAGAGGCAAACAACTTCCTA+TGG + chr8.1:80814913-80814932 MS.gene07777:CDS 45.0%
TGTTGGATCTCACTTCAGAG+AGG + chr8.1:80814896-80814915 MS.gene07777:CDS 45.0%
TTCTTCTTCATGCCACCCAA+AGG - chr8.1:80814957-80814976 None:intergenic 45.0%
TTGATCTACAAGCGCGGTTA+TGG + chr8.1:80814476-80814495 MS.gene07777:CDS 45.0%
TTGCAGGTTCTTGGGAAACA+TGG + chr8.1:80814834-80814853 MS.gene07777:intron 45.0%
TTTATGCATCTACGGAGGTC+AGG + chr8.1:80814036-80814055 MS.gene07777:intron 45.0%
! GCGGTTATGGAAAGGTTGAT+AGG + chr8.1:80814489-80814508 MS.gene07777:CDS 45.0%
! TATGTTGAAGGTGGTGATGC+TGG + chr8.1:80814987-80815006 MS.gene07777:CDS 45.0%
! TTTTCCAGGGAATCCCTCAT+TGG + chr8.1:80814156-80814175 MS.gene07777:intron 45.0%
AAGCTCAAAGCTCCTTTGGG+TGG + chr8.1:80814942-80814961 MS.gene07777:CDS 50.0%
ACATCCAATGAGGGATTCCC+TGG - chr8.1:80814163-80814182 None:intergenic 50.0%
ATAAGCTAGAGCAGCAGCCA+AGG + chr8.1:80813871-80813890 MS.gene07777:CDS 50.0%
CGTGAAGCTAAGCTTAAGGG+TGG + chr8.1:80814421-80814440 MS.gene07777:CDS 50.0%
CTACAAGCGCGGTTATGGAA+AGG + chr8.1:80814481-80814500 MS.gene07777:CDS 50.0%
CTTGACCAATGTCCCCTGTA+TGG + chr8.1:80814738-80814757 MS.gene07777:intron 50.0%
! AAGGTGGTGATGCTGGTAAC+AGG + chr8.1:80814994-80815013 MS.gene07777:CDS 50.0%
! AGGTGGTGATGCTGGTAACA+GGG + chr8.1:80814995-80815014 MS.gene07777:CDS 50.0%
CACCACCATACAGGGGACAT+TGG - chr8.1:80814746-80814765 None:intergenic 55.0%
GACCAATGTCCCCTGTATGG+TGG + chr8.1:80814741-80814760 MS.gene07777:intron 55.0%
! TGGTGATGCTGGTAACAGGG+AGG + chr8.1:80814998-80815017 MS.gene07777:CDS 55.0%
GAGGTCAGGAGAGGCTACAG+AGG + chr8.1:80814050-80814069 MS.gene07777:intron 60.0%
GCATCTACGGAGGTCAGGAG+AGG + chr8.1:80814041-80814060 MS.gene07777:intron 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 80813789 80815055 80813789 ID=MS.gene07777
chr8.1 mRNA 80813789 80815055 80813789 ID=MS.gene07777.t1;Parent=MS.gene07777
chr8.1 exon 80813789 80813892 80813789 ID=MS.gene07777.t1.exon1;Parent=MS.gene07777.t1
chr8.1 CDS 80813789 80813892 80813789 ID=cds.MS.gene07777.t1;Parent=MS.gene07777.t1
chr8.1 exon 80814053 80814115 80814053 ID=MS.gene07777.t1.exon2;Parent=MS.gene07777.t1
chr8.1 CDS 80814053 80814115 80814053 ID=cds.MS.gene07777.t1;Parent=MS.gene07777.t1
chr8.1 exon 80814421 80814550 80814421 ID=MS.gene07777.t1.exon3;Parent=MS.gene07777.t1
chr8.1 CDS 80814421 80814550 80814421 ID=cds.MS.gene07777.t1;Parent=MS.gene07777.t1
chr8.1 exon 80814840 80815055 80814840 ID=MS.gene07777.t1.exon4;Parent=MS.gene07777.t1
chr8.1 CDS 80814840 80815055 80814840 ID=cds.MS.gene07777.t1;Parent=MS.gene07777.t1
Gene Sequence

>MS.gene07777

ATGCTTAATGATCGTGTTCGCACTTGTAGAGTCTTAAAACATCGCAGAATCAGCTTTGTATACTATTGTGCTTGGGATTTGGATAAGCTAGAGCAGCAGCCAAGGTTGTATTTTAAGTTTGAATTAACTCAATTTTGGTTCTTAGAATATTTTCTTTCATGTGGAAGGATGCATACATGCTAGCATTCTAGTAGGATAACTTATTTATATTTAATTTTCTTTACACTATCATATAATAGTGACATGGTTTATGCATCTACGGAGGTCAGGAGAGGCTACAGAGGTTTTTGCATCTACAGAGAAAGCTGAGAAAGAACATGGTTGGAAGTAAGTACTTGTGTCTCGAATATTTTTCTTTGAAACATCCTTTTCCAGGGAATCCCTCATTGGATGTAGTTATCACAAGACCAATGAGATTCTTTCTTTGACTTATATATGGTAATCAACTCTGCTTTCATATTTGTTCTTGTATGGAAGATTTATAATTTAAAGTACGTACTTGTGGGTGTCTTTTACTCTTTATGTTTATCCAATTAATAGTGAGGGATGGTACTTTAATGTTTTAGAATGTTTTAGAATTTTAGAATGGTAAGGAATACGATGATTCGCAAAAGGAGTTGATTGCGTTGAAGCGTGAAGCTAAGCTTAAGGGTGGATTTTATGTTGATCCTGAAGAGTGTTAGAGAATTGATCTACAAGCGCGGTTATGGAAAGGTTGATAGGCAAAGAATTGCTTTGACAGATAACTCTATCATTGAACAGGTATGTCTTTGATTAGTATTCCCTGCTTTCATTCGATCACACAGTTGTCAATCTGAACTGTATGACATAGATGGGATCTCGCTTCATTCCGACTCTGTACTTTACCCAATTTGCCAAAGTCAGAATGTGAGTTGTTTAAACTTGACTAGACAGTCTAGATTACGTGACTTTGTCTAATGTGTAATTTCTTGACCAATGTCCCCTGTATGGTGGTGCTATTATTATTGTTGAAGGTTATGTTGCCGGTTAAATTTTTCTATTTTTCATCTGACATAGTGTTCAATTGCAGGTTCTTGGGAAACATGGTATCATTTGCATTGAAGATCTCATCCACGAGATCATGACTGTTGGATCTCACTTCAGAGAGGCAAACAACTTCCTATGGCCTTTCAAGCTCAAAGCTCCTTTGGGTGGCATGAAGAAGAAGATAAATCACTATGTTGAAGGTGGTGATGCTGGTAACAGGGAGGATTACATCAATGAACTCATTAGAAGGATGAATTAG

Protein sequence

>MS.gene07777.t1

MLNDRVRTCRVLKHRRISFVYYCAWDLDKLEQQPRSGEATEVFASTEKAEKEHGWNVKLSLRVDFMLILKSVRELIYKRGYGKVDRQRIALTDNSIIEQVLGKHGIICIEDLIHEIMTVGSHFREANNFLWPFKLKAPLGGMKKKINHYVEGGDAGNREDYINELIRRMN