Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20224.t1 | KEH21251.1 | 97.9 | 869 | 18 | 0 | 1 | 869 | 1 | 869 | 0.00E+00 | 1523.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20224.t1 | Q5SMU7 | 68.7 | 674 | 204 | 3 | 197 | 869 | 126 | 793 | 7.0e-276 | 951.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20224.t1 | A0A072TVQ3 | 97.9 | 869 | 18 | 0 | 1 | 869 | 1 | 869 | 0.0e+00 | 1523.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene20224.t1 | TR | PHD |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049251 | MS.gene20224 | 0.820939 | 5.26E-53 | -1.69E-46 |
| MS.gene049397 | MS.gene20224 | 0.814976 | 1.17E-51 | -1.69E-46 |
| MS.gene049596 | MS.gene20224 | 0.857321 | 1.77E-62 | -1.69E-46 |
| MS.gene049622 | MS.gene20224 | 0.801673 | 8.06E-49 | -1.69E-46 |
| MS.gene04985 | MS.gene20224 | 0.819496 | 1.13E-52 | -1.69E-46 |
| MS.gene049989 | MS.gene20224 | 0.894865 | 1.65E-75 | -1.69E-46 |
| MS.gene050297 | MS.gene20224 | 0.863762 | 1.98E-64 | -1.69E-46 |
| MS.gene050476 | MS.gene20224 | 0.83186 | 1.31E-55 | -1.69E-46 |
| MS.gene050544 | MS.gene20224 | 0.828398 | 9.19E-55 | -1.69E-46 |
| MS.gene050648 | MS.gene20224 | 0.813921 | 2.00E-51 | -1.69E-46 |
| MS.gene050875 | MS.gene20224 | 0.822034 | 2.94E-53 | -1.69E-46 |
| MS.gene05124 | MS.gene20224 | 0.881675 | 1.97E-70 | -1.69E-46 |
| MS.gene051263 | MS.gene20224 | 0.817605 | 3.02E-52 | -1.69E-46 |
| MS.gene051423 | MS.gene20224 | 0.813993 | 1.93E-51 | -1.69E-46 |
| MS.gene051629 | MS.gene20224 | 0.839421 | 1.60E-57 | -1.69E-46 |
| MS.gene051742 | MS.gene20224 | 0.829226 | 5.79E-55 | -1.69E-46 |
| MS.gene051816 | MS.gene20224 | 0.866474 | 2.78E-65 | -1.69E-46 |
| MS.gene051896 | MS.gene20224 | 0.835574 | 1.55E-56 | -1.69E-46 |
| MS.gene051914 | MS.gene20224 | 0.879522 | 1.16E-69 | -1.69E-46 |
| MS.gene051955 | MS.gene20224 | 0.84301 | 1.82E-58 | -1.69E-46 |
| MS.gene052143 | MS.gene20224 | 0.848413 | 6.23E-60 | -1.69E-46 |
| MS.gene052169 | MS.gene20224 | 0.837747 | 4.33E-57 | -1.69E-46 |
| MS.gene052308 | MS.gene20224 | 0.876912 | 9.57E-69 | -1.69E-46 |
| MS.gene052367 | MS.gene20224 | 0.907323 | 5.73E-81 | -1.69E-46 |
| MS.gene053173 | MS.gene20224 | 0.913 | 1.02E-83 | -1.69E-46 |
| MS.gene053348 | MS.gene20224 | 0.880122 | 7.12E-70 | -1.69E-46 |
| MS.gene053416 | MS.gene20224 | 0.902166 | 1.28E-78 | -1.69E-46 |
| MS.gene053417 | MS.gene20224 | 0.899632 | 1.63E-77 | -1.69E-46 |
| MS.gene05369 | MS.gene20224 | 0.839147 | 1.89E-57 | -1.69E-46 |
| MS.gene053929 | MS.gene20224 | 0.801497 | 8.76E-49 | -1.69E-46 |
| MS.gene054459 | MS.gene20224 | 0.844435 | 7.57E-59 | -1.69E-46 |
| MS.gene054551 | MS.gene20224 | 0.907901 | 3.07E-81 | -1.69E-46 |
| MS.gene054552 | MS.gene20224 | 0.845349 | 4.29E-59 | -1.69E-46 |
| MS.gene054593 | MS.gene20224 | 0.901583 | 2.31E-78 | -1.69E-46 |
| MS.gene054633 | MS.gene20224 | 0.865158 | 7.23E-65 | -1.69E-46 |
| MS.gene054643 | MS.gene20224 | 0.810168 | 1.32E-50 | -1.69E-46 |
| MS.gene054662 | MS.gene20224 | 0.880518 | 5.14E-70 | -1.69E-46 |
| MS.gene054885 | MS.gene20224 | 0.877205 | 7.57E-69 | -1.69E-46 |
| MS.gene055308 | MS.gene20224 | 0.890801 | 7.09E-74 | -1.69E-46 |
| MS.gene055411 | MS.gene20224 | 0.833798 | 4.34E-56 | -1.69E-46 |
| MS.gene055578 | MS.gene20224 | 0.876631 | 1.20E-68 | -1.69E-46 |
| MS.gene055579 | MS.gene20224 | 0.810461 | 1.14E-50 | -1.69E-46 |
| MS.gene055957 | MS.gene20224 | 0.875094 | 4.04E-68 | -1.69E-46 |
| MS.gene056041 | MS.gene20224 | 0.90641 | 1.53E-80 | -1.69E-46 |
| MS.gene056042 | MS.gene20224 | 0.816626 | 5.02E-52 | -1.69E-46 |
| MS.gene05640 | MS.gene20224 | 0.934326 | 4.80E-96 | -1.69E-46 |
| MS.gene056526 | MS.gene20224 | 0.888068 | 8.19E-73 | -1.69E-46 |
| MS.gene056528 | MS.gene20224 | 0.85199 | 6.19E-61 | -1.69E-46 |
| MS.gene05652 | MS.gene20224 | 0.840134 | 1.04E-57 | -1.69E-46 |
| MS.gene05732 | MS.gene20224 | 0.853474 | 2.33E-61 | -1.69E-46 |
| MS.gene057358 | MS.gene20224 | 0.808298 | 3.32E-50 | -1.69E-46 |
| MS.gene057367 | MS.gene20224 | 0.863669 | 2.11E-64 | -1.69E-46 |
| MS.gene057458 | MS.gene20224 | 0.911651 | 4.78E-83 | -1.69E-46 |
| MS.gene057669 | MS.gene20224 | 0.82674 | 2.30E-54 | -1.69E-46 |
| MS.gene058013 | MS.gene20224 | 0.818112 | 2.32E-52 | -1.69E-46 |
| MS.gene058128 | MS.gene20224 | 0.848362 | 6.43E-60 | -1.69E-46 |
| MS.gene058818 | MS.gene20224 | 0.85089 | 1.27E-60 | -1.69E-46 |
| MS.gene058827 | MS.gene20224 | 0.856775 | 2.56E-62 | -1.69E-46 |
| MS.gene058828 | MS.gene20224 | 0.824574 | 7.50E-54 | -1.69E-46 |
| MS.gene058844 | MS.gene20224 | 0.845879 | 3.08E-59 | -1.69E-46 |
| MS.gene058849 | MS.gene20224 | 0.844821 | 5.96E-59 | -1.69E-46 |
| MS.gene058853 | MS.gene20224 | 0.881633 | 2.04E-70 | -1.69E-46 |
| MS.gene059300 | MS.gene20224 | 0.822458 | 2.34E-53 | -1.69E-46 |
| MS.gene059553 | MS.gene20224 | 0.910071 | 2.82E-82 | -1.69E-46 |
| MS.gene059616 | MS.gene20224 | 0.824821 | 6.56E-54 | -1.69E-46 |
| MS.gene060275 | MS.gene20224 | 0.829424 | 5.19E-55 | -1.69E-46 |
| MS.gene060473 | MS.gene20224 | 0.908835 | 1.11E-81 | -1.69E-46 |
| MS.gene060565 | MS.gene20224 | 0.907156 | 6.87E-81 | -1.69E-46 |
| MS.gene06057 | MS.gene20224 | 0.81746 | 3.26E-52 | -1.69E-46 |
| MS.gene06093 | MS.gene20224 | 0.980473 | 2.67E-150 | -1.69E-46 |
| MS.gene061099 | MS.gene20224 | 0.862671 | 4.30E-64 | -1.69E-46 |
| MS.gene061260 | MS.gene20224 | 0.914754 | 1.32E-84 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20224.t1 | MTR_8g099640 | 98.044 | 869 | 17 | 0 | 1 | 869 | 1 | 869 | 0.0 | 1780 |
| MS.gene20224.t1 | MTR_3g060290 | 87.805 | 82 | 7 | 3 | 165 | 245 | 17 | 96 | 3.15e-32 | 122 |
| MS.gene20224.t1 | MTR_3g463490 | 30.855 | 269 | 166 | 7 | 493 | 754 | 80 | 335 | 4.62e-27 | 116 |
| MS.gene20224.t1 | MTR_3g463490 | 30.855 | 269 | 166 | 7 | 493 | 754 | 80 | 335 | 7.46e-27 | 115 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20224.t1 | AT4G14700 | 65.818 | 746 | 211 | 10 | 130 | 869 | 81 | 788 | 0.0 | 967 |
| MS.gene20224.t1 | AT4G12620 | 70.606 | 677 | 180 | 6 | 197 | 869 | 131 | 792 | 0.0 | 958 |
| MS.gene20224.t1 | AT1G07270 | 28.707 | 317 | 210 | 10 | 490 | 799 | 110 | 417 | 3.97e-30 | 125 |
| MS.gene20224.t1 | AT1G07270 | 28.707 | 317 | 210 | 10 | 490 | 799 | 110 | 417 | 5.00e-30 | 125 |
| MS.gene20224.t1 | AT2G29680 | 27.469 | 324 | 207 | 11 | 489 | 799 | 112 | 420 | 3.87e-26 | 114 |
| MS.gene20224.t1 | AT2G29680 | 25.070 | 355 | 207 | 12 | 489 | 799 | 112 | 451 | 7.23e-21 | 97.8 |
Find 211 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCGATGAATCTGGCGATTT+TGG | 0.133439 | 4.4:-4661542 | None:intergenic |
| GTTAGTTACCGACTGGTTTC+TGG | 0.202474 | 4.4:-4665013 | None:intergenic |
| TGAAGCTGCTGATAATTATA+TGG | 0.208304 | 4.4:-4665431 | None:intergenic |
| CTGCACAAGGGACGGTTCTT+TGG | 0.215974 | 4.4:+4664199 | MS.gene20224:CDS |
| CGCCCAACAGAAGTCTCTTC+TGG | 0.247491 | 4.4:-4662298 | None:intergenic |
| TCATCGGTTTGCATCATTAA+TGG | 0.257487 | 4.4:-4669859 | None:intergenic |
| CTCTATATTCACGGTGTTCC+GGG | 0.266627 | 4.4:+4664504 | MS.gene20224:CDS |
| TGGCAGCTGAAGAAGTAATA+AGG | 0.285779 | 4.4:-4669839 | None:intergenic |
| CCTCTATATTCACGGTGTTC+CGG | 0.288739 | 4.4:+4664503 | MS.gene20224:CDS |
| GTCATATATGAAGCATTAAA+TGG | 0.289669 | 4.4:+4664865 | MS.gene20224:CDS |
| CTTCAGGTTGCAGCTATATC+AGG | 0.290038 | 4.4:+4665635 | MS.gene20224:intron |
| TTTACAGATTTCGATGAATC+TGG | 0.295754 | 4.4:-4661551 | None:intergenic |
| AACCTACATTCCTCCACTTC+TGG | 0.299054 | 4.4:-4661869 | None:intergenic |
| CCGATTCTTCTCCCTCTTCT+CGG | 0.303926 | 4.4:-4661359 | None:intergenic |
| CTAGCTTTGGCATACTCCTT+AGG | 0.308499 | 4.4:-4663685 | None:intergenic |
| ATCTCCCTCATTGCTAGCTT+TGG | 0.313313 | 4.4:-4663698 | None:intergenic |
| TTCTTACCCTTCGGCGGCTT+TGG | 0.317699 | 4.4:-4662049 | None:intergenic |
| GGGAGATTGAAGAAGCTTCT+TGG | 0.333604 | 4.4:-4661198 | None:intergenic |
| AAAGAATTCCAGAGCATATA+AGG | 0.339598 | 4.4:+4664242 | MS.gene20224:CDS |
| TTTGTAGAAGGGAAGAAAAC+TGG | 0.345764 | 4.4:+4664934 | MS.gene20224:CDS |
| CTAAATGAGAGGTTTGTAGA+AGG | 0.346073 | 4.4:+4664922 | MS.gene20224:CDS |
| GGAGATCAATGGTCTGAAAT+TGG | 0.353511 | 4.4:+4664732 | MS.gene20224:CDS |
| ATTGAGTGTGATAGTTGTTT+AGG | 0.355173 | 4.4:+4661920 | MS.gene20224:CDS |
| TGAGATTCGGCAACGAGATT+GGG | 0.367135 | 4.4:-4661320 | None:intergenic |
| ATACCTGCAGCAACAATATC+AGG | 0.377832 | 4.4:-4665737 | None:intergenic |
| CCACATATCCAATCCCCTTC+AGG | 0.385042 | 4.4:-4661986 | None:intergenic |
| GCTGAATTGAAGAAGAGAAA+TGG | 0.386079 | 4.4:+4661602 | MS.gene20224:CDS |
| TAGCTTTGGCATACTCCTTA+GGG | 0.393164 | 4.4:-4663684 | None:intergenic |
| TTTCAACTCACTTGAATATG+AGG | 0.394384 | 4.4:-4668476 | None:intergenic |
| TTGAGATTCGGCAACGAGAT+TGG | 0.395152 | 4.4:-4661321 | None:intergenic |
| TATTTCTGACAATCAATGTC+TGG | 0.396034 | 4.4:+4664473 | MS.gene20224:CDS |
| ATAAGTCTTACCCAACTTGC+AGG | 0.400447 | 4.4:-4669532 | None:intergenic |
| AGAGTGACGGTGACGAAGAC+TGG | 0.402154 | 4.4:+4663976 | MS.gene20224:CDS |
| TTGATCTCCACAAAACAGTA+TGG | 0.411503 | 4.4:-4664718 | None:intergenic |
| AGGAGATGCACGCCGTGCTT+TGG | 0.412726 | 4.4:+4665655 | MS.gene20224:CDS |
| GGAGATTGAAGAAGCTTCTT+GGG | 0.412877 | 4.4:-4661197 | None:intergenic |
| CTAACAAGCTTCTTACCCTT+CGG | 0.416592 | 4.4:-4662058 | None:intergenic |
| GCTATATCGGACCAACGATT+TGG | 0.417826 | 4.4:+4662345 | MS.gene20224:CDS |
| TGAGAATTGTGAACGGAGGT+TGG | 0.424141 | 4.4:-4661231 | None:intergenic |
| AAAACGGTTGTTACAAGAGC+TGG | 0.428477 | 4.4:+4661719 | MS.gene20224:CDS |
| GAAGCTGCTGATAATTATAT+GGG | 0.430667 | 4.4:-4665430 | None:intergenic |
| ATTTGAGCAGAGAGCTATAT+CGG | 0.433378 | 4.4:+4662332 | MS.gene20224:CDS |
| TTCTTTGGACTTCAGAAGAT+AGG | 0.436400 | 4.4:+4664214 | MS.gene20224:CDS |
| ATGATATCCTCCTGCAAGTT+GGG | 0.437262 | 4.4:+4669522 | MS.gene20224:CDS |
| AGCAATGGTGAAGTGTTTCC+TGG | 0.439086 | 4.4:+4669497 | MS.gene20224:CDS |
| GAGCTTTACAAAACAGGAAT+GGG | 0.441059 | 4.4:+4669050 | MS.gene20224:CDS |
| CGGAGCGAACGAAACCTTAA+GGG | 0.442571 | 4.4:-4661636 | None:intergenic |
| TCCGACGTCGCCGGATCAAT+CGG | 0.442884 | 4.4:+4661655 | MS.gene20224:CDS |
| GACGGCGGTGAATTTGAAGT+TGG | 0.443655 | 4.4:+4661794 | MS.gene20224:CDS |
| GGGCGTTGCCTCTATATTCA+CGG | 0.455755 | 4.4:+4664495 | MS.gene20224:CDS |
| GGCTTCAATCAAGTGATTTG+TGG | 0.457739 | 4.4:+4662095 | MS.gene20224:CDS |
| CATTCCAAGCTAATTGTGAT+AGG | 0.457837 | 4.4:+4665159 | MS.gene20224:CDS |
| GATCCTGATATTGTTGCTGC+AGG | 0.457925 | 4.4:+4665734 | MS.gene20224:CDS |
| ATCATTTCGAGTCGCCTCAA+TGG | 0.464299 | 4.4:+4665458 | MS.gene20224:CDS |
| ACGGCGGTGAATTTGAAGTT+GGG | 0.465132 | 4.4:+4661795 | MS.gene20224:CDS |
| CTTACCTATCACAATTAGCT+TGG | 0.465486 | 4.4:-4665163 | None:intergenic |
| AAGATGTTCCTGAAGGGGAT+TGG | 0.466368 | 4.4:+4661978 | MS.gene20224:CDS |
| GGCAGCTGAAGAAGTAATAA+GGG | 0.468817 | 4.4:-4669838 | None:intergenic |
| GTGCACGAGCTTTACAAAAC+AGG | 0.469313 | 4.4:+4669044 | MS.gene20224:CDS |
| TGATTGTTGAATTTGAGATT+CGG | 0.470559 | 4.4:-4661333 | None:intergenic |
| TCGGAGCGAACGAAACCTTA+AGG | 0.473564 | 4.4:-4661637 | None:intergenic |
| TTTCTACAAGGTAAAGACTT+AGG | 0.474900 | 4.4:-4664322 | None:intergenic |
| TTGTGAGACCTTATATGCTC+TGG | 0.474919 | 4.4:-4664250 | None:intergenic |
| TTCTTAGTTTCCGATTGATC+CGG | 0.476110 | 4.4:-4661665 | None:intergenic |
| GCCCAACAGAAGTCTCTTCT+GGG | 0.476671 | 4.4:-4662297 | None:intergenic |
| ATACGAGGAAGTAACTTCTC+TGG | 0.476939 | 4.4:-4665371 | None:intergenic |
| TTCGTTCGCTCCGACGTCGC+CGG | 0.480213 | 4.4:+4661646 | MS.gene20224:CDS |
| GCTTCAATCAAGTGATTTGT+GGG | 0.480427 | 4.4:+4662096 | MS.gene20224:CDS |
| CCTGAAGGGGATTGGATATG+TGG | 0.480446 | 4.4:+4661986 | MS.gene20224:CDS |
| TGGGAAGGGAGATTCCCTTA+AGG | 0.483741 | 4.4:+4661622 | MS.gene20224:CDS |
| CTGAATTGAAGAAGAGAAAT+GGG | 0.485254 | 4.4:+4661603 | MS.gene20224:CDS |
| GAGTGTGATAGTTGTTTAGG+TGG | 0.490466 | 4.4:+4661923 | MS.gene20224:CDS |
| TCATGTCTCTGCGGCAGCAA+TGG | 0.493304 | 4.4:+4669482 | MS.gene20224:CDS |
| TGGTCTGAAATTGGCTTCAC+CGG | 0.494433 | 4.4:+4664741 | MS.gene20224:CDS |
| TTCTGTACAATATTCTTGAC+TGG | 0.495175 | 4.4:+4665124 | MS.gene20224:CDS |
| GCAGCTACTGGTGTCGGGTA+CGG | 0.495197 | 4.4:+4662263 | MS.gene20224:CDS |
| TAAATGAGAGGTTTGTAGAA+GGG | 0.497183 | 4.4:+4664923 | MS.gene20224:CDS |
| ATTCACGGTGTTCCGGGCAC+TGG | 0.498438 | 4.4:+4664510 | MS.gene20224:CDS |
| AACAATGCAGTTGGCAATGA+CGG | 0.500318 | 4.4:+4669457 | MS.gene20224:intron |
| GTGGCACAGTTTCAAACGCT+TGG | 0.500569 | 4.4:+4663756 | MS.gene20224:CDS |
| GTTACAAGAGCTGGGAGAAA+AGG | 0.500757 | 4.4:+4661728 | MS.gene20224:CDS |
| AAAGCCTCTGGATGCCCATT+CGG | 0.503391 | 4.4:-4665400 | None:intergenic |
| GGATGGCAGCTACTGGTGTC+GGG | 0.503839 | 4.4:+4662258 | MS.gene20224:CDS |
| TTCACCTTGTAAATATTCTC+CGG | 0.504027 | 4.4:-4664760 | None:intergenic |
| CCGAGAAGAGGGAGAAGAAT+CGG | 0.504286 | 4.4:+4661359 | MS.gene20224:CDS |
| GCAGCTAACTGATGGCTTGT+CGG | 0.504655 | 4.4:-4664083 | None:intergenic |
| TGAGGTCAAGGTTGAGAAAA+CGG | 0.504688 | 4.4:+4661703 | MS.gene20224:CDS |
| ATCTGTGAGGGTCGTAAGAT+GGG | 0.506846 | 4.4:+4662010 | MS.gene20224:CDS |
| CCTCGTATATCAAGCCGAAT+GGG | 0.511380 | 4.4:+4665386 | MS.gene20224:CDS |
| GATAGTACCTTGCCAGTGCC+CGG | 0.512164 | 4.4:-4664522 | None:intergenic |
| ATCCCAGAAGAGACTTCTGT+TGG | 0.512492 | 4.4:+4662295 | MS.gene20224:CDS |
| TCAAGACGAGATTTCGCAGC+TGG | 0.512532 | 4.4:-4661395 | None:intergenic |
| CGGAAACTAAGAAGAGAAAG+AGG | 0.512657 | 4.4:+4661675 | MS.gene20224:CDS |
| GATCTGTGAGGGTCGTAAGA+TGG | 0.515072 | 4.4:+4662009 | MS.gene20224:CDS |
| TTGAAGTCAGAAGTTGATGC+AGG | 0.515204 | 4.4:+4664685 | MS.gene20224:CDS |
| ATTTCTGACAATCAATGTCT+GGG | 0.516051 | 4.4:+4664474 | MS.gene20224:CDS |
| AGCTTGGAATGTGGCTTGGT+TGG | 0.517458 | 4.4:-4665147 | None:intergenic |
| CAAATATATCAATTCCATTG+AGG | 0.517649 | 4.4:-4665472 | None:intergenic |
| TGGAGCAAATGCAATTTCAT+CGG | 0.518476 | 4.4:-4661522 | None:intergenic |
| TACAAGAAAGTTGTTTATGA+CGG | 0.522606 | 4.4:+4661776 | MS.gene20224:CDS |
| GCATTAAATGGACACAGGGT+TGG | 0.523201 | 4.4:+4664877 | MS.gene20224:CDS |
| AAAGAGGAAGAATGAGGTCA+AGG | 0.524216 | 4.4:+4661691 | MS.gene20224:CDS |
| GGTAAGGAATGAGGAGTGAG+AGG | 0.524682 | 4.4:-4661254 | None:intergenic |
| CAAACCTCTCATTTAGCAAA+CGG | 0.525477 | 4.4:-4664915 | None:intergenic |
| ATGGGGCAAAGCAAAGCCTC+TGG | 0.529383 | 4.4:-4665412 | None:intergenic |
| AGCGTTTGAAACTGTGCCAC+TGG | 0.529464 | 4.4:-4663753 | None:intergenic |
| TCAACATACTTATTTCTACA+AGG | 0.530757 | 4.4:-4664334 | None:intergenic |
| TTGAAGAAGAGAAATGGGAA+GGG | 0.532289 | 4.4:+4661608 | MS.gene20224:CDS |
| GCTCGTGCACCATAGCTGTC+AGG | 0.532743 | 4.4:-4669031 | None:intergenic |
| CGTGGATCTACGTGTAGGTA+AGG | 0.533392 | 4.4:-4661270 | None:intergenic |
| CTCGATGTTGGCCAAATCGT+TGG | 0.533909 | 4.4:-4662356 | None:intergenic |
| TGGATGGCAGCTACTGGTGT+CGG | 0.533932 | 4.4:+4662257 | MS.gene20224:CDS |
| ATCTGTAAAGAAGAGGAAGC+AGG | 0.534383 | 4.4:+4661565 | MS.gene20224:CDS |
| TCACTTGAATATGAGGTGCT+TGG | 0.534848 | 4.4:-4668469 | None:intergenic |
| CGAGCTTTACAAAACAGGAA+TGG | 0.537464 | 4.4:+4669049 | MS.gene20224:CDS |
| AATTAGCTTGGAATGTGGCT+TGG | 0.540690 | 4.4:-4665151 | None:intergenic |
| CGGTGTTCCGGGCACTGGCA+AGG | 0.541081 | 4.4:+4664515 | MS.gene20224:CDS |
| ATCCAGAAGTGGAGGAATGT+AGG | 0.542570 | 4.4:+4661867 | MS.gene20224:CDS |
| GAGATTCGGCAACGAGATTG+GGG | 0.543738 | 4.4:-4661319 | None:intergenic |
| TAAATGGACACAGGGTTGGA+TGG | 0.543748 | 4.4:+4664881 | MS.gene20224:CDS |
| GTGAAGCAGGTGCCAAGCAC+AGG | 0.543759 | 4.4:+4669772 | MS.gene20224:CDS |
| AGTGTGATAGTTGTTTAGGT+GGG | 0.547500 | 4.4:+4661924 | MS.gene20224:CDS |
| CATGATATCCTCCTGCAAGT+TGG | 0.553301 | 4.4:+4669521 | MS.gene20224:CDS |
| CCGGAACACCGTGAATATAG+AGG | 0.553353 | 4.4:-4664503 | None:intergenic |
| CATCGAAATCTGTAAAGAAG+AGG | 0.553381 | 4.4:+4661558 | MS.gene20224:CDS |
| TCCTCGTATATCAAGCCGAA+TGG | 0.555101 | 4.4:+4665385 | MS.gene20224:CDS |
| TTCTTGGGAGTTGAAGCCAT+TGG | 0.556223 | 4.4:-4661182 | None:intergenic |
| GGGATGATGTTTATGTGAAG+AGG | 0.559397 | 4.4:+4661816 | MS.gene20224:CDS |
| TGCAGGAGGATATCATGTCC+AGG | 0.562882 | 4.4:-4669515 | None:intergenic |
| GAAGAGAAAGAGGAAGAATG+AGG | 0.565359 | 4.4:+4661685 | MS.gene20224:CDS |
| AACAATCAACTCCGAGAAGA+GGG | 0.566074 | 4.4:+4661348 | MS.gene20224:CDS |
| AGCTCTCTGCTCAAATTATG+TGG | 0.566453 | 4.4:-4662325 | None:intergenic |
| TTCATACTGCAGCTAACTGA+TGG | 0.566543 | 4.4:-4664091 | None:intergenic |
| ATTGAAGAAGAGAAATGGGA+AGG | 0.568468 | 4.4:+4661607 | MS.gene20224:CDS |
| TTGGATATGTGGGATCTGTG+AGG | 0.570339 | 4.4:+4661997 | MS.gene20224:CDS |
| ACAATCCATCACCTCGATGT+TGG | 0.575437 | 4.4:-4662368 | None:intergenic |
| CTCTCCGTTTGCTAAATGAG+AGG | 0.575524 | 4.4:+4664911 | MS.gene20224:CDS |
| TGTGGGCTGCTCGCATTGAG+AGG | 0.577481 | 4.4:+4662113 | MS.gene20224:CDS |
| AGGGAAGAAAACTGGAGAAG+AGG | 0.577588 | 4.4:+4664942 | MS.gene20224:CDS |
| TCCCAGAAGAGACTTCTGTT+GGG | 0.577921 | 4.4:+4662296 | MS.gene20224:CDS |
| AAGAAGTGGATGGCAGCTAC+TGG | 0.578137 | 4.4:+4662251 | MS.gene20224:CDS |
| GGGCTTTGAGAAATAACCTC+GGG | 0.578651 | 4.4:-4669818 | None:intergenic |
| CGTTGAAAGATGTTCCTGAA+GGG | 0.581179 | 4.4:+4661972 | MS.gene20224:CDS |
| TCCGATTGATCCGGCGACGT+CGG | 0.582582 | 4.4:-4661656 | None:intergenic |
| TATGCCAAAGCTAGCAATGA+GGG | 0.585809 | 4.4:+4663694 | MS.gene20224:CDS |
| TGAGAGGTGAGAATTGTGAA+CGG | 0.585848 | 4.4:-4661238 | None:intergenic |
| TTGCTATGTCATGATCCCTA+AGG | 0.591823 | 4.4:+4663669 | MS.gene20224:CDS |
| TTGTTAGAACAATGCGTCAG+AGG | 0.591921 | 4.4:+4662074 | MS.gene20224:CDS |
| CCGTTGAAAGATGTTCCTGA+AGG | 0.592540 | 4.4:+4661971 | MS.gene20224:CDS |
| CTGAAGGGGATTGGATATGT+GGG | 0.593658 | 4.4:+4661987 | MS.gene20224:CDS |
| ACACAGGGTTGGATGGAAAG+AGG | 0.594099 | 4.4:+4664888 | MS.gene20224:CDS |
| AGGAAAGGGACTTGTTTGCA+TGG | 0.594820 | 4.4:+4668415 | MS.gene20224:intron |
| ACAAGCTTCTTACCCTTCGG+CGG | 0.596059 | 4.4:-4662055 | None:intergenic |
| CAACAATCAACTCCGAGAAG+AGG | 0.596926 | 4.4:+4661347 | MS.gene20224:CDS |
| TAGCAGTATATGGAAAGAAG+TGG | 0.597595 | 4.4:+4662237 | MS.gene20224:intron |
| AAACGGTTGTTACAAGAGCT+GGG | 0.597984 | 4.4:+4661720 | MS.gene20224:CDS |
| GTTGAAAGATGTTCCTGAAG+GGG | 0.598838 | 4.4:+4661973 | MS.gene20224:CDS |
| ATGAAGCATTAAATGGACAC+AGG | 0.600808 | 4.4:+4664872 | MS.gene20224:CDS |
| TACAGAATCTGCACAAGGGA+CGG | 0.603574 | 4.4:+4664191 | MS.gene20224:intron |
| GCTGTAAGTTGGGTGAGTGT+AGG | 0.604738 | 4.4:+4669739 | MS.gene20224:intron |
| GACTAATCTTGAAAGAGCAA+AGG | 0.606188 | 4.4:+4664276 | MS.gene20224:CDS |
| GTCTGCATATTTCCAAAGCA+CGG | 0.606454 | 4.4:-4665667 | None:intergenic |
| TGGATATGTGGGATCTGTGA+GGG | 0.607873 | 4.4:+4661998 | MS.gene20224:CDS |
| TTCACCGGAGAATATTTACA+AGG | 0.608665 | 4.4:+4664756 | MS.gene20224:CDS |
| TTCAGGGATAGCAAATACAA+TGG | 0.610395 | 4.4:+4665343 | MS.gene20224:intron |
| AATTTCTGAAGAAGAGAAGT+CGG | 0.611112 | 4.4:+4661489 | MS.gene20224:CDS |
| CGGCGGTGAATTTGAAGTTG+GGG | 0.617237 | 4.4:+4661796 | MS.gene20224:CDS |
| CCTTCAGGAACATCTTTCAA+CGG | 0.617438 | 4.4:-4661971 | None:intergenic |
| AGAAGATGCTACTTCTGATG+AGG | 0.621654 | 4.4:+4661841 | MS.gene20224:CDS |
| ACAAGAGCTGGGAGAAAAGG+TGG | 0.622286 | 4.4:+4661731 | MS.gene20224:CDS |
| TCGTCAATGAGCAAAATACA+TGG | 0.624876 | 4.4:-4664973 | None:intergenic |
| TGAGGAAGATCCAGAAGTGG+AGG | 0.628093 | 4.4:+4661859 | MS.gene20224:CDS |
| GTATGCCAAAGCTAGCAATG+AGG | 0.629062 | 4.4:+4663693 | MS.gene20224:CDS |
| CTACGTGTAGGTAAGGAATG+AGG | 0.635826 | 4.4:-4661263 | None:intergenic |
| CAATGAGCAAAATACATGGT+CGG | 0.635961 | 4.4:-4664969 | None:intergenic |
| CCCATTCGGCTTGATATACG+AGG | 0.638148 | 4.4:-4665386 | None:intergenic |
| TGAAGCATTAAATGGACACA+GGG | 0.640411 | 4.4:+4664873 | MS.gene20224:CDS |
| AGTATCGGAGTTCAAGCGCG+TGG | 0.641574 | 4.4:-4661288 | None:intergenic |
| AAGCGCGTGGATCTACGTGT+AGG | 0.642274 | 4.4:-4661275 | None:intergenic |
| GCTACTGGTGTCGGGTACGG+TGG | 0.642777 | 4.4:+4662266 | MS.gene20224:CDS |
| GCTAACTGATGGCTTGTCGG+TGG | 0.645325 | 4.4:-4664080 | None:intergenic |
| TGATGAGGAAGATCCAGAAG+TGG | 0.647235 | 4.4:+4661856 | MS.gene20224:CDS |
| AGGGCTTTGAGAAATAACCT+CGG | 0.647966 | 4.4:-4669819 | None:intergenic |
| ATCACAATTAGCTTGGAATG+TGG | 0.648587 | 4.4:-4665156 | None:intergenic |
| AGTATATGGAAAGAAGTGGA+TGG | 0.649668 | 4.4:+4662241 | MS.gene20224:intron |
| AGATTCGGCAACGAGATTGG+GGG | 0.651554 | 4.4:-4661318 | None:intergenic |
| TCATGACATAGCAATGTCGA+AGG | 0.658256 | 4.4:-4663660 | None:intergenic |
| AAGAAAGTTGTTTATGACGG+CGG | 0.658330 | 4.4:+4661779 | MS.gene20224:CDS |
| GAGATAACGACATTCATCAA+AGG | 0.658884 | 4.4:+4664447 | MS.gene20224:CDS |
| TGAGTGTACTCTCAGTGATG+AGG | 0.663135 | 4.4:+4664659 | MS.gene20224:CDS |
| TCTTGTAACCAGAAACCAGT+CGG | 0.664804 | 4.4:+4665005 | MS.gene20224:CDS |
| CGAAGACTGGAACATCGACA+AGG | 0.665993 | 4.4:+4663989 | MS.gene20224:CDS |
| AAGCTGCTGATAATTATATG+GGG | 0.670932 | 4.4:-4665429 | None:intergenic |
| GAGGTGAGAATTGTGAACGG+AGG | 0.673704 | 4.4:-4661235 | None:intergenic |
| TGACATTGATGATGAAAGAG+AGG | 0.674539 | 4.4:+4663780 | MS.gene20224:CDS |
| AGTCTTACCCAACTTGCAGG+AGG | 0.676386 | 4.4:-4669529 | None:intergenic |
| TCAGGAACATCTTTCAACGG+TGG | 0.680289 | 4.4:-4661968 | None:intergenic |
| GCTTTACAAAACAGGAATGG+GGG | 0.682677 | 4.4:+4669052 | MS.gene20224:CDS |
| AGCTTTACAAAACAGGAATG+GGG | 0.683635 | 4.4:+4669051 | MS.gene20224:CDS |
| AAAAGGTGGAAAGATTGCGA+AGG | 0.684724 | 4.4:+4661745 | MS.gene20224:CDS |
| AATGATACAGAATCTGCACA+AGG | 0.686949 | 4.4:+4664186 | MS.gene20224:intron |
| GTAAAGAAGAGGAAGCAGGA+AGG | 0.690620 | 4.4:+4661569 | MS.gene20224:CDS |
| AGTTGCAGCTCAATTTCCCG+AGG | 0.692840 | 4.4:+4669802 | MS.gene20224:CDS |
| TGAAGATGTTGAATATGATG+AGG | 0.693036 | 4.4:+4664031 | MS.gene20224:CDS |
| CAACGATTTGGCCAACATCG+AGG | 0.693338 | 4.4:+4662357 | MS.gene20224:CDS |
| TTTCTGACAATCAATGTCTG+GGG | 0.698842 | 4.4:+4664475 | MS.gene20224:CDS |
| CAAGCCGAATGGGCATCCAG+AGG | 0.706358 | 4.4:+4665396 | MS.gene20224:CDS |
| AATATGAATATGACATCCAG+TGG | 0.707312 | 4.4:+4663737 | MS.gene20224:CDS |
| ATGACGGTTTCATGTCTCTG+CGG | 0.719836 | 4.4:+4669473 | MS.gene20224:CDS |
| ATGATACAGAATCTGCACAA+GGG | 0.728041 | 4.4:+4664187 | MS.gene20224:intron |
| TAGAATTCTGATGAGAGTGA+CGG | 0.741239 | 4.4:+4663963 | MS.gene20224:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 4661185 | 4669881 | 4661185 | ID=MS.gene20224 |
| chr4.4 | mRNA | 4661185 | 4669881 | 4661185 | ID=MS.gene20224.t1;Parent=MS.gene20224 |
| chr4.4 | exon | 4661185 | 4662134 | 4661185 | ID=MS.gene20224.t1.exon1;Parent=MS.gene20224.t1 |
| chr4.4 | CDS | 4661185 | 4662134 | 4661185 | ID=cds.MS.gene20224.t1;Parent=MS.gene20224.t1 |
| chr4.4 | exon | 4662243 | 4662378 | 4662243 | ID=MS.gene20224.t1.exon2;Parent=MS.gene20224.t1 |
| chr4.4 | CDS | 4662243 | 4662378 | 4662243 | ID=cds.MS.gene20224.t1;Parent=MS.gene20224.t1 |
| chr4.4 | exon | 4663649 | 4663801 | 4663649 | ID=MS.gene20224.t1.exon3;Parent=MS.gene20224.t1 |
| chr4.4 | CDS | 4663649 | 4663801 | 4663649 | ID=cds.MS.gene20224.t1;Parent=MS.gene20224.t1 |
| chr4.4 | exon | 4663966 | 4664106 | 4663966 | ID=MS.gene20224.t1.exon4;Parent=MS.gene20224.t1 |
| chr4.4 | CDS | 4663966 | 4664106 | 4663966 | ID=cds.MS.gene20224.t1;Parent=MS.gene20224.t1 |
| chr4.4 | exon | 4664196 | 4664347 | 4664196 | ID=MS.gene20224.t1.exon5;Parent=MS.gene20224.t1 |
| chr4.4 | CDS | 4664196 | 4664347 | 4664196 | ID=cds.MS.gene20224.t1;Parent=MS.gene20224.t1 |
| chr4.4 | exon | 4664437 | 4664536 | 4664437 | ID=MS.gene20224.t1.exon6;Parent=MS.gene20224.t1 |
| chr4.4 | CDS | 4664437 | 4664536 | 4664437 | ID=cds.MS.gene20224.t1;Parent=MS.gene20224.t1 |
| chr4.4 | exon | 4664655 | 4664777 | 4664655 | ID=MS.gene20224.t1.exon7;Parent=MS.gene20224.t1 |
| chr4.4 | CDS | 4664655 | 4664777 | 4664655 | ID=cds.MS.gene20224.t1;Parent=MS.gene20224.t1 |
| chr4.4 | exon | 4664865 | 4665026 | 4664865 | ID=MS.gene20224.t1.exon8;Parent=MS.gene20224.t1 |
| chr4.4 | CDS | 4664865 | 4665026 | 4664865 | ID=cds.MS.gene20224.t1;Parent=MS.gene20224.t1 |
| chr4.4 | exon | 4665123 | 4665180 | 4665123 | ID=MS.gene20224.t1.exon9;Parent=MS.gene20224.t1 |
| chr4.4 | CDS | 4665123 | 4665180 | 4665123 | ID=cds.MS.gene20224.t1;Parent=MS.gene20224.t1 |
| chr4.4 | exon | 4665348 | 4665538 | 4665348 | ID=MS.gene20224.t1.exon10;Parent=MS.gene20224.t1 |
| chr4.4 | CDS | 4665348 | 4665538 | 4665348 | ID=cds.MS.gene20224.t1;Parent=MS.gene20224.t1 |
| chr4.4 | exon | 4665641 | 4665755 | 4665641 | ID=MS.gene20224.t1.exon11;Parent=MS.gene20224.t1 |
| chr4.4 | CDS | 4665641 | 4665755 | 4665641 | ID=cds.MS.gene20224.t1;Parent=MS.gene20224.t1 |
| chr4.4 | exon | 4668417 | 4668487 | 4668417 | ID=MS.gene20224.t1.exon12;Parent=MS.gene20224.t1 |
| chr4.4 | CDS | 4668417 | 4668487 | 4668417 | ID=cds.MS.gene20224.t1;Parent=MS.gene20224.t1 |
| chr4.4 | exon | 4668996 | 4669091 | 4668996 | ID=MS.gene20224.t1.exon13;Parent=MS.gene20224.t1 |
| chr4.4 | CDS | 4668996 | 4669091 | 4668996 | ID=cds.MS.gene20224.t1;Parent=MS.gene20224.t1 |
| chr4.4 | exon | 4669467 | 4669543 | 4669467 | ID=MS.gene20224.t1.exon14;Parent=MS.gene20224.t1 |
| chr4.4 | CDS | 4669467 | 4669543 | 4669467 | ID=cds.MS.gene20224.t1;Parent=MS.gene20224.t1 |
| chr4.4 | exon | 4669740 | 4669881 | 4669740 | ID=MS.gene20224.t1.exon15;Parent=MS.gene20224.t1 |
| chr4.4 | CDS | 4669740 | 4669881 | 4669740 | ID=cds.MS.gene20224.t1;Parent=MS.gene20224.t1 |
| Gene Sequence |
| Protein sequence |
