AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20264


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20264.t1 MTR_5g016150 96.992 266 8 0 1 266 1 266 0.0 542
MS.gene20264.t1 MTR_4g095310 80.000 265 53 0 1 265 1 265 1.61e-167 464
MS.gene20264.t1 MTR_1g018320 57.028 249 105 2 6 253 8 255 2.27e-107 312
MS.gene20264.t1 MTR_3g045440 39.184 245 147 2 9 252 13 256 8.32e-68 211
MS.gene20264.t1 MTR_6g086560 40.157 254 149 3 9 260 22 274 1.14e-65 206
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20264.t1 AT4G37470 78.113 265 58 0 1 265 1 265 4.85e-160 446
MS.gene20264.t1 AT3G03990 58.468 248 102 1 6 253 8 254 7.79e-108 313
MS.gene20264.t1 AT3G24420 42.742 248 139 3 7 252 13 259 2.55e-75 231

Find 57 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTAAACTTGTCATGGTTTC+CGG 0.180279 5.1:+8443593 MS.gene20264:CDS
CAGCGATAGCGTTGAATAAT+TGG 0.189658 5.1:-8444287 None:intergenic
TTATGATTTACTTGCTATTT+TGG 0.190068 5.1:+8443478 MS.gene20264:CDS
CGGAAACCATGACAAGTTTA+AGG 0.259764 5.1:-8443592 None:intergenic
CTCTCAGAATCAAAGTATTC+AGG 0.261092 5.1:-8443431 None:intergenic
GGCGGTTCCGGTGGTGGTTT+CGG 0.295547 5.1:+8444521 MS.gene20264:CDS
ATAGTTCTAGCTCATGGATT+TGG 0.302677 5.1:+8443314 MS.gene20264:CDS
CTCACCAGATATTGTTATTC+CGG 0.306476 5.1:+8444620 MS.gene20264:CDS
CGCTCCACTGGCTGTGGGTG+GGG 0.319640 5.1:+8444338 MS.gene20264:CDS
AATTACAAAGCATGGTGTTA+TGG 0.329086 5.1:+8444312 MS.gene20264:CDS
GAGAGACATTCAAGCTTAGA+AGG 0.335053 5.1:+8443449 MS.gene20264:CDS
TACTAATTACCTTGGTGAAC+CGG 0.348114 5.1:-8443612 None:intergenic
CATTCTGTTTCTGCAATGAT+TGG 0.356191 5.1:+8443536 MS.gene20264:CDS
ACTGGCTGTGGGTGGGGACA+TGG 0.358536 5.1:+8444344 MS.gene20264:CDS
TGAGAAAGATATGGCGGTTC+CGG 0.374023 5.1:+8444509 MS.gene20264:CDS
ATTGAATCTTGCGTATTTGT+TGG 0.380353 5.1:+8443512 MS.gene20264:CDS
AACACCGGAATAACAATATC+TGG 0.403561 5.1:-8444624 None:intergenic
TTGAACGATGTGAATTACTT+TGG 0.403798 5.1:+8444240 MS.gene20264:CDS
TCGCTCCACTGGCTGTGGGT+GGG 0.418335 5.1:+8444337 MS.gene20264:CDS
TGGATTCGCTCCACTGGCTG+TGG 0.437225 5.1:+8444332 MS.gene20264:CDS
CAAAATGTTTCCACACAGAT+TGG 0.437928 5.1:-8443343 None:intergenic
CATGGATTCAGTGGCAGTAC+AGG 0.447432 5.1:+8444362 MS.gene20264:CDS
TTCGCTCCACTGGCTGTGGG+TGG 0.454426 5.1:+8444336 MS.gene20264:CDS
AGATATGGCGGTTCCGGTGG+TGG 0.469390 5.1:+8444515 MS.gene20264:CDS
CGAAAATCATCAACAAGATA+AGG 0.479354 5.1:-8443368 None:intergenic
ATCAAAGTATTCAGGGTTAG+TGG 0.490058 5.1:-8443423 None:intergenic
TATACAAGCTGAGAAAGATA+TGG 0.495978 5.1:+8444500 MS.gene20264:CDS
GAGTATCTTCACCAACACTT+AGG 0.503482 5.1:+8444543 MS.gene20264:CDS
GTGTTATGGATTCGCTCCAC+TGG 0.516025 5.1:+8444326 MS.gene20264:CDS
TCTCAGAATCAAAGTATTCA+GGG 0.516412 5.1:-8443430 None:intergenic
AGGTACATAGTTCTAGCTCA+TGG 0.516768 5.1:+8443308 MS.gene20264:CDS
AACGATGTGAATTACTTTGG+AGG 0.518896 5.1:+8444243 MS.gene20264:CDS
ACAAGTTTAAGGAAAAGATC+AGG 0.522449 5.1:-8443581 None:intergenic
GACACTATTAGAGCTATGTC+TGG 0.532117 5.1:-8444414 None:intergenic
TCGCTGAAAATTACAAAGCA+TGG 0.559131 5.1:+8444304 MS.gene20264:CDS
TGTTGTTCTATATGATAACA+TGG 0.566458 5.1:+8443391 MS.gene20264:CDS
GGATTCGCTCCACTGGCTGT+GGG 0.566939 5.1:+8444333 MS.gene20264:CDS
ATGTCCCCACCCACAGCCAG+TGG 0.567376 5.1:-8444342 None:intergenic
CTATATGATAACATGGGTGC+TGG 0.576349 5.1:+8443398 MS.gene20264:CDS
GTCGAACATTGTTCAACATG+AGG 0.582241 5.1:+8444391 MS.gene20264:CDS
AGGTGGTCAGTCCATTGTTG+AGG 0.589895 5.1:+8444563 MS.gene20264:CDS
GAAAGATATGGCGGTTCCGG+TGG 0.592732 5.1:+8444512 MS.gene20264:CDS
TGAATGTGTTTAAGCAACAC+CGG 0.601873 5.1:-8444639 None:intergenic
GGGTGGGGACATGGATTCAG+TGG 0.603042 5.1:+8444353 MS.gene20264:CDS
TATCTTCACCAACACTTAGG+TGG 0.616011 5.1:+8444546 MS.gene20264:CDS
AGTCTGCATGACCTCAACAA+TGG 0.633137 5.1:-8444574 None:intergenic
TATGACAAGGCACAGTAACA+AGG 0.637592 5.1:-8444476 None:intergenic
GTTGAGGTCATGCAGACTGA+TGG 0.648337 5.1:+8444579 MS.gene20264:CDS
AGATACTCCGAAACCACCAC+CGG 0.658384 5.1:-8444528 None:intergenic
ACATAGCTCTAATAGTGTCA+CGG 0.660027 5.1:+8444418 MS.gene20264:CDS
TCATGGTTTCCGGTTCACCA+AGG 0.661388 5.1:+8443603 MS.gene20264:CDS
ACAAGCTGAGAAAGATATGG+CGG 0.694733 5.1:+8444503 MS.gene20264:CDS
TCAGCTTGTATAATATGACA+AGG 0.695896 5.1:-8444489 None:intergenic
TGGACTGACCACCTAAGTGT+TGG 0.706295 5.1:-8444554 None:intergenic
TTGGAACAGACCAATCTGTG+TGG 0.709650 5.1:+8443333 MS.gene20264:CDS
GTTGTTCTATATGATAACAT+GGG 0.717956 5.1:+8443392 MS.gene20264:CDS
ATATCTGGTGAGCTTAACTG+CGG 0.718673 5.1:-8444609 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTGTAACATTGTTTAA+TGG + chr5.1:8443661-8443680 MS.gene20264:intron 15.0%
!! AATATGAAATTTGATATAGT+TGG + chr5.1:8444051-8444070 MS.gene20264:intron 15.0%
!! AAATAATCAAGAGAAATGTA+AGG - chr5.1:8443871-8443890 None:intergenic 20.0%
!! AATAATCAAGAGAAATGTAA+GGG - chr5.1:8443870-8443889 None:intergenic 20.0%
!! ATAAAACTTACTAATTACCT+TGG - chr5.1:8443623-8443642 None:intergenic 20.0%
!! TGAACAATAAACACAAAATT+TGG + chr5.1:8443693-8443712 MS.gene20264:intron 20.0%
!! TTATTATTGTTAAGCAACTT+AGG - chr5.1:8444120-8444139 None:intergenic 20.0%
!!! AGATTTTAAAAAAGGGAAAA+TGG + chr5.1:8443761-8443780 MS.gene20264:intron 20.0%
!!! TTATGATTTACTTGCTATTT+TGG + chr5.1:8443478-8443497 MS.gene20264:CDS 20.0%
!!! TTTTAGACATAGTTTGAATT+TGG + chr5.1:8443929-8443948 MS.gene20264:intron 20.0%
! AAGAACATAAAACATCTCAA+TGG - chr5.1:8443998-8444017 None:intergenic 25.0%
! AATGATATCAAATTGAACCT+AGG - chr5.1:8443828-8443847 None:intergenic 25.0%
! GTTGTTCTATATGATAACAT+GGG + chr5.1:8443392-8443411 MS.gene20264:CDS 25.0%
! TCGACTATAAGTATATAGTT+TGG + chr5.1:8443896-8443915 MS.gene20264:intron 25.0%
! TGAACCTAGGTATAAAAAAA+AGG - chr5.1:8443815-8443834 None:intergenic 25.0%
! TGTTGTTCTATATGATAACA+TGG + chr5.1:8443391-8443410 MS.gene20264:CDS 25.0%
!! TTTTATATGTGATTGTGACA+TGG + chr5.1:8444192-8444211 MS.gene20264:intron 25.0%
!!! AGGAGTAACAATATTTTAAG+TGG - chr5.1:8443795-8443814 None:intergenic 25.0%
!!! GTTTATTTTCTCTTTGAGTT+AGG + chr5.1:8444214-8444233 MS.gene20264:intron 25.0%
!!! TACTCCTTTTTTTTATACCT+AGG + chr5.1:8443808-8443827 MS.gene20264:intron 25.0%
ACAAGTTTAAGGAAAAGATC+AGG - chr5.1:8443584-8443603 None:intergenic 30.0%
ATTGAATCTTGCGTATTTGT+TGG + chr5.1:8443512-8443531 MS.gene20264:CDS 30.0%
CGAAAATCATCAACAAGATA+AGG - chr5.1:8443371-8443390 None:intergenic 30.0%
TATACAAGCTGAGAAAGATA+TGG + chr5.1:8444500-8444519 MS.gene20264:CDS 30.0%
TATTGTTAAGCAACTTAGGT+AGG - chr5.1:8444116-8444135 None:intergenic 30.0%
TCAATCAATTCAACTTTAGC+CGG + chr5.1:8443958-8443977 MS.gene20264:intron 30.0%
TCAGCTTGTATAATATGACA+AGG - chr5.1:8444492-8444511 None:intergenic 30.0%
TTGAACGATGTGAATTACTT+TGG + chr5.1:8444240-8444259 MS.gene20264:CDS 30.0%
! AATTACAAAGCATGGTGTTA+TGG + chr5.1:8444312-8444331 MS.gene20264:CDS 30.0%
! TCTCAGAATCAAAGTATTCA+GGG - chr5.1:8443433-8443452 None:intergenic 30.0%
! TCTTTTCCTTAAACTTGTCA+TGG + chr5.1:8443586-8443605 MS.gene20264:CDS 30.0%
!!! AAGTTTTAGGAACAGGAAAT+AGG + chr5.1:8443288-8443307 MS.gene20264:CDS 30.0%
AACACCGGAATAACAATATC+TGG - chr5.1:8444627-8444646 None:intergenic 35.0%
AACGATGTGAATTACTTTGG+AGG + chr5.1:8444243-8444262 MS.gene20264:CDS 35.0%
CAAAATGTTTCCACACAGAT+TGG - chr5.1:8443346-8443365 None:intergenic 35.0%
CAAGAGAAATGTAAGGGTTA+TGG - chr5.1:8443864-8443883 None:intergenic 35.0%
CAATCAATTCAACTTTAGCC+GGG + chr5.1:8443959-8443978 MS.gene20264:intron 35.0%
CTTAAACTTGTCATGGTTTC+CGG + chr5.1:8443593-8443612 MS.gene20264:CDS 35.0%
TACTAATTACCTTGGTGAAC+CGG - chr5.1:8443615-8443634 None:intergenic 35.0%
TCGCTGAAAATTACAAAGCA+TGG + chr5.1:8444304-8444323 MS.gene20264:CDS 35.0%
TGAATGTGTTTAAGCAACAC+CGG - chr5.1:8444642-8444661 None:intergenic 35.0%
! ATCAAAGTATTCAGGGTTAG+TGG - chr5.1:8443426-8443445 None:intergenic 35.0%
! CTCACCAGATATTGTTATTC+CGG + chr5.1:8444620-8444639 MS.gene20264:CDS 35.0%
! CTCTCAGAATCAAAGTATTC+AGG - chr5.1:8443434-8443453 None:intergenic 35.0%
!! ACATAGCTCTAATAGTGTCA+CGG + chr5.1:8444418-8444437 MS.gene20264:CDS 35.0%
!! ATAGTTCTAGCTCATGGATT+TGG + chr5.1:8443314-8443333 MS.gene20264:CDS 35.0%
!! CATTCTGTTTCTGCAATGAT+TGG + chr5.1:8443536-8443555 MS.gene20264:CDS 35.0%
!!! AACGTGAAAGTTTTAGGAAC+AGG + chr5.1:8443281-8443300 MS.gene20264:CDS 35.0%
AATGGTATCAAGTGTCTACC+CGG - chr5.1:8443980-8443999 None:intergenic 40.0%
ACAAGCTGAGAAAGATATGG+CGG + chr5.1:8444503-8444522 MS.gene20264:CDS 40.0%
ATATCTGGTGAGCTTAACTG+CGG - chr5.1:8444612-8444631 None:intergenic 40.0%
CGGAAACCATGACAAGTTTA+AGG - chr5.1:8443595-8443614 None:intergenic 40.0%
CTATATGATAACATGGGTGC+TGG + chr5.1:8443398-8443417 MS.gene20264:CDS 40.0%
GACACTATTAGAGCTATGTC+TGG - chr5.1:8444417-8444436 None:intergenic 40.0%
GAGAGACATTCAAGCTTAGA+AGG + chr5.1:8443449-8443468 MS.gene20264:CDS 40.0%
GAGTATCTTCACCAACACTT+AGG + chr5.1:8444543-8444562 MS.gene20264:CDS 40.0%
GTCGAACATTGTTCAACATG+AGG + chr5.1:8444391-8444410 MS.gene20264:CDS 40.0%
TATCTTCACCAACACTTAGG+TGG + chr5.1:8444546-8444565 MS.gene20264:CDS 40.0%
TATGACAAGGCACAGTAACA+AGG - chr5.1:8444479-8444498 None:intergenic 40.0%
TGTTCTTGTTGCTAACGTGT+CGG + chr5.1:8444011-8444030 MS.gene20264:intron 40.0%
! CAGCGATAGCGTTGAATAAT+TGG - chr5.1:8444290-8444309 None:intergenic 40.0%
! GCTCACAACGTGAAAGTTTT+AGG + chr5.1:8443275-8443294 MS.gene20264:CDS 40.0%
!! AGGTACATAGTTCTAGCTCA+TGG + chr5.1:8443308-8443327 MS.gene20264:CDS 40.0%
AGTCTGCATGACCTCAACAA+TGG - chr5.1:8444577-8444596 None:intergenic 45.0%
ATTGTGTGTGTGACAGTGTG+AGG - chr5.1:8443726-8443745 None:intergenic 45.0%
TGAGAAAGATATGGCGGTTC+CGG + chr5.1:8444509-8444528 MS.gene20264:CDS 45.0%
TTGGAACAGACCAATCTGTG+TGG + chr5.1:8443333-8443352 MS.gene20264:CDS 45.0%
!!! ATAAAATAGATTTTAAAAAA+GGG + chr5.1:8443754-8443773 MS.gene20264:intron 5.0%
!!! TATAAAATAGATTTTAAAAA+AGG + chr5.1:8443753-8443772 MS.gene20264:intron 5.0%
!!! TTTTTTTTTTTTTTTTTTGA+CGG + chr5.1:8444666-8444685 MS.gene20264:CDS 5.0%
AGATACTCCGAAACCACCAC+CGG - chr5.1:8444531-8444550 None:intergenic 50.0%
AGGTGGTCAGTCCATTGTTG+AGG + chr5.1:8444563-8444582 MS.gene20264:CDS 50.0%
CATGGATTCAGTGGCAGTAC+AGG + chr5.1:8444362-8444381 MS.gene20264:CDS 50.0%
GTGTTATGGATTCGCTCCAC+TGG + chr5.1:8444326-8444345 MS.gene20264:CDS 50.0%
GTTGAGGTCATGCAGACTGA+TGG + chr5.1:8444579-8444598 MS.gene20264:CDS 50.0%
TCATGGTTTCCGGTTCACCA+AGG + chr5.1:8443603-8443622 MS.gene20264:CDS 50.0%
TGGACTGACCACCTAAGTGT+TGG - chr5.1:8444557-8444576 None:intergenic 50.0%
GAAAGATATGGCGGTTCCGG+TGG + chr5.1:8444512-8444531 MS.gene20264:CDS 55.0%
GTGTGTGTGACAGTGTGAGG+CGG - chr5.1:8443723-8443742 None:intergenic 55.0%
AGATATGGCGGTTCCGGTGG+TGG + chr5.1:8444515-8444534 MS.gene20264:CDS 60.0%
GGGTGGGGACATGGATTCAG+TGG + chr5.1:8444353-8444372 MS.gene20264:CDS 60.0%
! GGATTCGCTCCACTGGCTGT+GGG + chr5.1:8444333-8444352 MS.gene20264:CDS 60.0%
! TGGATTCGCTCCACTGGCTG+TGG + chr5.1:8444332-8444351 MS.gene20264:CDS 60.0%
ACTGGCTGTGGGTGGGGACA+TGG + chr5.1:8444344-8444363 MS.gene20264:CDS 65.0%
ATGTCCCCACCCACAGCCAG+TGG - chr5.1:8444345-8444364 None:intergenic 65.0%
! GGCGGTTCCGGTGGTGGTTT+CGG + chr5.1:8444521-8444540 MS.gene20264:CDS 65.0%
! TCGCTCCACTGGCTGTGGGT+GGG + chr5.1:8444337-8444356 MS.gene20264:CDS 65.0%
! TTCGCTCCACTGGCTGTGGG+TGG + chr5.1:8444336-8444355 MS.gene20264:CDS 65.0%
! CGCTCCACTGGCTGTGGGTG+GGG + chr5.1:8444338-8444357 MS.gene20264:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 8443257 8444698 8443257 ID=MS.gene20264
chr5.1 mRNA 8443257 8444698 8443257 ID=MS.gene20264.t1;Parent=MS.gene20264
chr5.1 exon 8443257 8443624 8443257 ID=MS.gene20264.t1.exon1;Parent=MS.gene20264.t1
chr5.1 CDS 8443257 8443624 8443257 ID=cds.MS.gene20264.t1;Parent=MS.gene20264.t1
chr5.1 exon 8444236 8444698 8444236 ID=MS.gene20264.t1.exon2;Parent=MS.gene20264.t1
chr5.1 CDS 8444236 8444698 8444236 ID=cds.MS.gene20264.t1;Parent=MS.gene20264.t1
Gene Sequence

>MS.gene20264

ATGGGAATAGTGGAAGAAGCTCACAACGTGAAAGTTTTAGGAACAGGAAATAGGTACATAGTTCTAGCTCATGGATTTGGAACAGACCAATCTGTGTGGAAACATTTTGTACCTTATCTTGTTGATGATTTTCGTGTTGTTCTATATGATAACATGGGTGCTGGTACCACTAACCCTGAATACTTTGATTCTGAGAGACATTCAAGCTTAGAAGGTTATGCTTATGATTTACTTGCTATTTTGGAAGAGCTTCAAATTGAATCTTGCGTATTTGTTGGTCATTCTGTTTCTGCAATGATTGGTGCTATTGCTTCTATTACACGACCTGATCTTTTCCTTAAACTTGTCATGGTTTCCGGTTCACCAAGGTAATTAGTAAGTTTTATTTGTACAGTTTATACATAAAAATTGTAACATTGTTTAATGGTGAATTATTTGAACAATAAACACAAAATTTGGAGCACCGCCTCACACTGTCACACACACAATATATATGTATAAAATAGATTTTAAAAAAGGGAAAATGGATTTGTGTCCACTTAAAATATTGTTACTCCTTTTTTTTATACCTAGGTTCAATTTGATATCATTGAGAATTTTTTTTCCATAACCCTTACATTTCTCTTGATTATTTTGAATTCGACTATAAGTATATAGTTTGGTAAAATATTCTTTTAGACATAGTTTGAATTTGGATACTCTCAATCAATTCAACTTTAGCCGGGTAGACACTTGATACCATTGAGATGTTTTATGTTCTTGTTGCTAACGTGTCGGAAAAGACATGCTCTAGTAATATGAAATTTGATATAGTTGGATAAAGAATTCTTTTATAACTTGAATACTTTGAATCGATCCTACCTAAGTTGCTTAACAATAATAATAATAATTTTAAATTATATGCATAAAAAATTTATTAGCTAACTGTAAAACTATTTTATATGTGATTGTGACATGGTTTATTTTCTCTTTGAGTTAGGTATTTGAACGATGTGAATTACTTTGGAGGATTTGAGCAAGAAGATTTGAACCAATTATTCAACGCTATCGCTGAAAATTACAAAGCATGGTGTTATGGATTCGCTCCACTGGCTGTGGGTGGGGACATGGATTCAGTGGCAGTACAGGAGTTTAGTCGAACATTGTTCAACATGAGGCCAGACATAGCTCTAATAGTGTCACGGACAATTTTTCAAAGTGACATGAGACAGATTTTGAACCTTGTTACTGTGCCTTGTCATATTATACAAGCTGAGAAAGATATGGCGGTTCCGGTGGTGGTTTCGGAGTATCTTCACCAACACTTAGGTGGTCAGTCCATTGTTGAGGTCATGCAGACTGATGGTCATTTGCCGCAGTTAAGCTCACCAGATATTGTTATTCCGGTGTTGCTTAAACACATTCAACTTTTTTTTTTTTTTTTTTTTGACGGTAAACAATAG

Protein sequence

>MS.gene20264.t1

MGIVEEAHNVKVLGTGNRYIVLAHGFGTDQSVWKHFVPYLVDDFRVVLYDNMGAGTTNPEYFDSERHSSLEGYAYDLLAILEELQIESCVFVGHSVSAMIGAIASITRPDLFLKLVMVSGSPRYLNDVNYFGGFEQEDLNQLFNAIAENYKAWCYGFAPLAVGGDMDSVAVQEFSRTLFNMRPDIALIVSRTIFQSDMRQILNLVTVPCHIIQAEKDMAVPVVVSEYLHQHLGGQSIVEVMQTDGHLPQLSSPDIVIPVLLKHIQLFFFFFFDGKQ