Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21886.t1 | XP_024640983.1 | 98.7 | 463 | 6 | 0 | 1 | 463 | 1 | 463 | 8.60E-269 | 935.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21886.t1 | Q54PW5 | 46.2 | 411 | 174 | 6 | 31 | 438 | 103 | 469 | 4.5e-109 | 396.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21886.t1 | G7K6J3 | 98.7 | 463 | 6 | 0 | 1 | 463 | 1 | 463 | 6.2e-269 | 935.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene21886 | 0.810481 | 1.13E-50 | -1.69E-46 |
| MS.gene049207 | MS.gene21886 | 0.815204 | 1.04E-51 | -1.69E-46 |
| MS.gene049254 | MS.gene21886 | 0.803295 | 3.73E-49 | -1.69E-46 |
| MS.gene049283 | MS.gene21886 | 0.804452 | 2.15E-49 | -1.69E-46 |
| MS.gene049983 | MS.gene21886 | 0.812783 | 3.56E-51 | -1.69E-46 |
| MS.gene050559 | MS.gene21886 | 0.800979 | 1.12E-48 | -1.69E-46 |
| MS.gene051198 | MS.gene21886 | 0.826854 | 2.16E-54 | -1.69E-46 |
| MS.gene051363 | MS.gene21886 | 0.82522 | 5.28E-54 | -1.69E-46 |
| MS.gene051597 | MS.gene21886 | 0.804442 | 2.16E-49 | -1.69E-46 |
| MS.gene051975 | MS.gene21886 | 0.809085 | 2.25E-50 | -1.69E-46 |
| MS.gene052108 | MS.gene21886 | 0.803473 | 3.43E-49 | -1.69E-46 |
| MS.gene052500 | MS.gene21886 | 0.80302 | 4.25E-49 | -1.69E-46 |
| MS.gene052582 | MS.gene21886 | 0.810105 | 1.36E-50 | -1.69E-46 |
| MS.gene052584 | MS.gene21886 | 0.822171 | 2.73E-53 | -1.69E-46 |
| MS.gene052826 | MS.gene21886 | 0.801223 | 9.96E-49 | -1.69E-46 |
| MS.gene052954 | MS.gene21886 | 0.831951 | 1.25E-55 | -1.69E-46 |
| MS.gene053006 | MS.gene21886 | 0.818774 | 1.64E-52 | -1.69E-46 |
| MS.gene053008 | MS.gene21886 | 0.822024 | 2.96E-53 | -1.69E-46 |
| MS.gene053058 | MS.gene21886 | 0.834191 | 3.46E-56 | -1.69E-46 |
| MS.gene053079 | MS.gene21886 | 0.800753 | 1.24E-48 | -1.69E-46 |
| MS.gene053080 | MS.gene21886 | 0.833256 | 5.92E-56 | -1.69E-46 |
| MS.gene05308 | MS.gene21886 | 0.812343 | 4.45E-51 | -1.69E-46 |
| MS.gene053226 | MS.gene21886 | 0.838358 | 3.02E-57 | -1.69E-46 |
| MS.gene05364 | MS.gene21886 | 0.80101 | 1.10E-48 | -1.69E-46 |
| MS.gene05415 | MS.gene21886 | 0.802818 | 4.68E-49 | -1.69E-46 |
| MS.gene054479 | MS.gene21886 | 0.820364 | 7.13E-53 | -1.69E-46 |
| MS.gene054651 | MS.gene21886 | 0.822943 | 1.81E-53 | -1.69E-46 |
| MS.gene054657 | MS.gene21886 | 0.823482 | 1.35E-53 | -1.69E-46 |
| MS.gene054658 | MS.gene21886 | 0.801321 | 9.51E-49 | -1.69E-46 |
| MS.gene054994 | MS.gene21886 | 0.812774 | 3.58E-51 | -1.69E-46 |
| MS.gene055166 | MS.gene21886 | 0.811465 | 6.91E-51 | -1.69E-46 |
| MS.gene05517 | MS.gene21886 | 0.817939 | 2.54E-52 | -1.69E-46 |
| MS.gene055222 | MS.gene21886 | 0.833195 | 6.13E-56 | -1.69E-46 |
| MS.gene055623 | MS.gene21886 | 0.812531 | 4.05E-51 | -1.69E-46 |
| MS.gene055778 | MS.gene21886 | 0.810983 | 8.79E-51 | -1.69E-46 |
| MS.gene055841 | MS.gene21886 | 0.825562 | 4.38E-54 | -1.69E-46 |
| MS.gene056215 | MS.gene21886 | 0.801135 | 1.04E-48 | -1.69E-46 |
| MS.gene056403 | MS.gene21886 | 0.814115 | 1.82E-51 | -1.69E-46 |
| MS.gene056506 | MS.gene21886 | 0.807725 | 4.39E-50 | -1.69E-46 |
| MS.gene05659 | MS.gene21886 | 0.800057 | 1.72E-48 | -1.69E-46 |
| MS.gene056605 | MS.gene21886 | 0.819968 | 8.79E-53 | -1.69E-46 |
| MS.gene056776 | MS.gene21886 | 0.816462 | 5.46E-52 | -1.69E-46 |
| MS.gene056973 | MS.gene21886 | 0.807974 | 3.89E-50 | -1.69E-46 |
| MS.gene057208 | MS.gene21886 | 0.832662 | 8.32E-56 | -1.69E-46 |
| MS.gene057314 | MS.gene21886 | 0.824696 | 7.02E-54 | -1.69E-46 |
| MS.gene057693 | MS.gene21886 | 0.814797 | 1.28E-51 | -1.69E-46 |
| MS.gene057869 | MS.gene21886 | 0.810813 | 9.56E-51 | -1.69E-46 |
| MS.gene057915 | MS.gene21886 | 0.80949 | 1.84E-50 | -1.69E-46 |
| MS.gene058010 | MS.gene21886 | 0.805599 | 1.23E-49 | -1.69E-46 |
| MS.gene058126 | MS.gene21886 | 0.956468 | 2.75E-114 | -1.69E-46 |
| MS.gene058548 | MS.gene21886 | 0.847921 | 8.52E-60 | -1.69E-46 |
| MS.gene058549 | MS.gene21886 | 0.820159 | 7.95E-53 | -1.69E-46 |
| MS.gene058781 | MS.gene21886 | 0.802542 | 5.34E-49 | -1.69E-46 |
| MS.gene058783 | MS.gene21886 | 0.809694 | 1.67E-50 | -1.69E-46 |
| MS.gene058979 | MS.gene21886 | 0.823854 | 1.11E-53 | -1.69E-46 |
| MS.gene05954 | MS.gene21886 | 0.813784 | 2.15E-51 | -1.69E-46 |
| MS.gene05978 | MS.gene21886 | 0.836988 | 6.77E-57 | -1.69E-46 |
| MS.gene05979 | MS.gene21886 | 0.846161 | 2.58E-59 | -1.69E-46 |
| MS.gene059830 | MS.gene21886 | 0.807838 | 4.16E-50 | -1.69E-46 |
| MS.gene059842 | MS.gene21886 | 0.824491 | 7.84E-54 | -1.69E-46 |
| MS.gene059935 | MS.gene21886 | 0.807628 | 4.61E-50 | -1.69E-46 |
| MS.gene060310 | MS.gene21886 | 0.807848 | 4.14E-50 | -1.69E-46 |
| MS.gene060429 | MS.gene21886 | 0.805279 | 1.44E-49 | -1.69E-46 |
| MS.gene060803 | MS.gene21886 | 0.804866 | 1.76E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene21886 | 0.818958 | 1.49E-52 | -1.69E-46 |
| MS.gene060937 | MS.gene21886 | 0.812939 | 3.29E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21886.t1 | MTR_5g007970 | 98.704 | 463 | 6 | 0 | 1 | 463 | 1 | 463 | 0.0 | 951 |
| MS.gene21886.t1 | MTR_1g008370 | 23.797 | 374 | 223 | 10 | 64 | 383 | 126 | 491 | 1.70e-18 | 88.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21886.t1 | AT5G62030 | 70.312 | 448 | 118 | 5 | 10 | 450 | 6 | 445 | 0.0 | 639 |
| MS.gene21886.t1 | AT3G59630 | 24.706 | 425 | 251 | 13 | 55 | 432 | 9 | 411 | 2.52e-24 | 105 |
| MS.gene21886.t1 | AT3G59630 | 24.706 | 425 | 251 | 13 | 55 | 432 | 9 | 411 | 2.52e-24 | 105 |
Find 123 sgRNAs with CRISPR-Local
Find 154 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTAAGGATTTAGGGTTTA+TGG | 0.120386 | 5.1:-2187918 | MS.gene21886:CDS |
| GGGACTATTCAGTTTGCTTC+TGG | 0.141078 | 5.1:-2187875 | MS.gene21886:CDS |
| AAGTTGAATTCTAAGGATTT+AGG | 0.180160 | 5.1:-2187926 | MS.gene21886:CDS |
| ACAAGGGAATCCTAAGATTT+TGG | 0.190611 | 5.1:-2187504 | MS.gene21886:CDS |
| GCTGAGATTCTTATTCATTA+TGG | 0.202025 | 5.1:-2188046 | MS.gene21886:CDS |
| GGAGAGATTGGAAATGAAAA+TGG | 0.217434 | 5.1:-2187483 | MS.gene21886:CDS |
| TTCAGTTTGCTTCTGGGATT+AGG | 0.232646 | 5.1:-2187868 | MS.gene21886:CDS |
| TACCCTAATTTCTCCAATTC+AGG | 0.242150 | 5.1:+2187836 | None:intergenic |
| AAGCCTGAATTGGAGAAATT+AGG | 0.248444 | 5.1:-2187839 | MS.gene21886:CDS |
| ATTTAGGGTTTATGGGTAAA+AGG | 0.253235 | 5.1:-2187910 | MS.gene21886:CDS |
| TCCAATCTCTCCAAAATCTT+AGG | 0.253400 | 5.1:+2187494 | None:intergenic |
| GAAATTAGGGTATAGGGTTT+TGG | 0.278652 | 5.1:-2187825 | MS.gene21886:CDS |
| TCAGTTTGCTTCTGGGATTA+GGG | 0.286217 | 5.1:-2187867 | MS.gene21886:CDS |
| TCCTAGACTCTCTATTGATT+GGG | 0.286257 | 5.1:-2187357 | MS.gene21886:CDS |
| TCAAACAAAGAGATTCTATA+AGG | 0.295399 | 5.1:+2187413 | None:intergenic |
| GCCGAGGCGACTGAAGATTT+TGG | 0.298442 | 5.1:-2187173 | MS.gene21886:CDS |
| AGTTGAATTCTAAGGATTTA+GGG | 0.301959 | 5.1:-2187925 | MS.gene21886:CDS |
| TACTGTTAAGTTGAATTCTA+AGG | 0.302039 | 5.1:-2187933 | MS.gene21886:CDS |
| GATAGCGAATCCGGGGATTA+AGG | 0.306937 | 5.1:-2187666 | MS.gene21886:CDS |
| GGACTATTCAGTTTGCTTCT+GGG | 0.318873 | 5.1:-2187874 | MS.gene21886:CDS |
| GAGTACATGAGAAGGCCTTC+AGG | 0.322311 | 5.1:+2188184 | None:intergenic |
| TCTAAGGATTTAGGGTTTAT+GGG | 0.324406 | 5.1:-2187917 | MS.gene21886:CDS |
| ATTGGAGAAATTAGGGTATA+GGG | 0.325559 | 5.1:-2187831 | MS.gene21886:CDS |
| AATTGGAGAAATTAGGGTAT+AGG | 0.337620 | 5.1:-2187832 | MS.gene21886:CDS |
| GTCCTAGACTCTCTATTGAT+TGG | 0.343341 | 5.1:-2187358 | MS.gene21886:CDS |
| GGTAAAAGGGTTATTCTTGC+TGG | 0.344947 | 5.1:-2187896 | MS.gene21886:CDS |
| TGAGGATTGAATCTGGGATT+TGG | 0.359458 | 5.1:+2188323 | None:intergenic |
| GATTTCTCTGCTATTGCTCT+TGG | 0.366141 | 5.1:-2188070 | MS.gene21886:CDS |
| AGCGAAGGAGGCAAGGAATT+GGG | 0.371820 | 5.1:-2187552 | MS.gene21886:CDS |
| CAAGGTTGTGAAGATGTTAC+AGG | 0.372583 | 5.1:-2187239 | MS.gene21886:CDS |
| TCCAACTCTTTCCCCACCTT+CGG | 0.380494 | 5.1:+2187752 | None:intergenic |
| TCGAAGTTCACAAATGCGTT+TGG | 0.385078 | 5.1:-2188249 | MS.gene21886:CDS |
| GAGAGATTGGAAATGAAAAT+GGG | 0.385615 | 5.1:-2187482 | MS.gene21886:CDS |
| AGCCTGAATTGGAGAAATTA+GGG | 0.386228 | 5.1:-2187838 | MS.gene21886:CDS |
| AGCGTTTATGATAGCGAATC+CGG | 0.400296 | 5.1:-2187675 | MS.gene21886:CDS |
| CAAACAAAGAGATTCTATAA+GGG | 0.414212 | 5.1:+2187414 | None:intergenic |
| AAGCGAAGGAGGCAAGGAAT+TGG | 0.416971 | 5.1:-2187553 | MS.gene21886:CDS |
| GGGTCGTTGAGGATTGAATC+TGG | 0.417738 | 5.1:+2188316 | None:intergenic |
| GTAACTCACTGTTACGTTCT+CGG | 0.423153 | 5.1:-2188124 | MS.gene21886:CDS |
| TAGGGCTGTGAAGCCTGAAT+TGG | 0.423463 | 5.1:-2187849 | MS.gene21886:CDS |
| ACTTCGAAGGAGTAGTTTGA+TGG | 0.426404 | 5.1:+2188265 | None:intergenic |
| GGGGAAAGAGTTGGATGATA+AGG | 0.427023 | 5.1:-2187744 | MS.gene21886:CDS |
| CAGTCGAAGCCGTTGTCTGC+TGG | 0.443942 | 5.1:-2187797 | MS.gene21886:CDS |
| CTCTTTGTTTGAAGATTCTG+TGG | 0.444587 | 5.1:-2187402 | MS.gene21886:CDS |
| AGCGATGCGTTTAGCGGCGG+TGG | 0.450582 | 5.1:+2188216 | None:intergenic |
| GCCTCAAAAGGAGTCAGAAC+TGG | 0.454686 | 5.1:+2187317 | None:intergenic |
| TTGAAGATTCTGTGGATGCT+TGG | 0.461951 | 5.1:-2187394 | MS.gene21886:CDS |
| TTTAGGGTTTATGGGTAAAA+GGG | 0.464356 | 5.1:-2187909 | MS.gene21886:CDS |
| GTTGCTCTAACGGTAGCTGC+GGG | 0.466214 | 5.1:-2187199 | MS.gene21886:CDS |
| AGAAACAGTGATGGTGTTGT+TGG | 0.468978 | 5.1:+2188384 | None:intergenic |
| TATGATCATGTGGGTATGAA+GGG | 0.470330 | 5.1:-2187599 | MS.gene21886:CDS |
| ATATGATCATGTGGGTATGA+AGG | 0.476747 | 5.1:-2187600 | MS.gene21886:CDS |
| TGATAAGGAAGAGAGTGTTT+TGG | 0.478103 | 5.1:-2187729 | MS.gene21886:CDS |
| AGGTTGTTCTGGAAATTCCA+TGG | 0.486487 | 5.1:+2188414 | None:intergenic |
| TGGTGTTTGTGTCGGATGGA+AGG | 0.488027 | 5.1:-2187709 | MS.gene21886:CDS |
| TGCGTTTAGCGGCGGTGGAA+CGG | 0.489236 | 5.1:+2188221 | None:intergenic |
| GCGTTTATGATAGCGAATCC+GGG | 0.502842 | 5.1:-2187674 | MS.gene21886:CDS |
| AGTGCTATCTCAGCCTCAAA+AGG | 0.509062 | 5.1:+2187305 | None:intergenic |
| AAAACAACTTCCCCACATAA+GGG | 0.512334 | 5.1:+2187630 | None:intergenic |
| GTAAAAGGGTTATTCTTGCT+GGG | 0.516662 | 5.1:-2187895 | MS.gene21886:CDS |
| TAGGAAAATTTCAGTAACTT+CGG | 0.516766 | 5.1:-2187069 | MS.gene21886:CDS |
| ACCAAAATCTTCAGTCGCCT+CGG | 0.518601 | 5.1:+2187172 | None:intergenic |
| GAAACAGTGATGGTGTTGTT+GGG | 0.521747 | 5.1:+2188385 | None:intergenic |
| GATATCTTCACCACCTTCGC+CGG | 0.525270 | 5.1:-2188148 | MS.gene21886:CDS |
| AGTACATGAGAAGGCCTTCA+GGG | 0.526196 | 5.1:+2188185 | None:intergenic |
| AGGGTGTACTGCACCGAAGG+TGG | 0.530199 | 5.1:-2187765 | MS.gene21886:CDS |
| CGGTAGCTGCGGGGATGCCG+AGG | 0.530356 | 5.1:-2187189 | MS.gene21886:CDS |
| GACTATTATGCTCAAGATGG+AGG | 0.530446 | 5.1:-2187134 | MS.gene21886:CDS |
| CGAAGGAGTAGTTTGATGGA+AGG | 0.531570 | 5.1:+2188269 | None:intergenic |
| TTTAGGTATGACCCTTATGT+GGG | 0.532658 | 5.1:-2187641 | MS.gene21886:CDS |
| TGATGGAAGGATTGAGATTG+CGG | 0.540466 | 5.1:+2188282 | None:intergenic |
| TACCTAAAAGCCTTAATCCC+CGG | 0.540870 | 5.1:+2187656 | None:intergenic |
| AGATTGCGGCGTTGAGAAGT+GGG | 0.549039 | 5.1:+2188296 | None:intergenic |
| GAGATTGCGGCGTTGAGAAG+TGG | 0.551098 | 5.1:+2188295 | None:intergenic |
| AAGAGCGATTGTTGCTCTAA+CGG | 0.551801 | 5.1:-2187209 | MS.gene21886:CDS |
| GTTCTCGGTGATGTTACCTA+TGG | 0.552000 | 5.1:-2188109 | MS.gene21886:CDS |
| GGGTGTACTGCACCGAAGGT+GGG | 0.552210 | 5.1:-2187764 | MS.gene21886:CDS |
| GGTCGTTGAGGATTGAATCT+GGG | 0.555765 | 5.1:+2188317 | None:intergenic |
| AACGCATTTGTGAACTTCGA+AGG | 0.558188 | 5.1:+2188252 | None:intergenic |
| GGTGTACTGCACCGAAGGTG+GGG | 0.558556 | 5.1:-2187763 | MS.gene21886:CDS |
| TTGGAGGAATATGATCATGT+GGG | 0.563003 | 5.1:-2187608 | MS.gene21886:CDS |
| CGTTGAGAAGTGGGTCGTTG+AGG | 0.563741 | 5.1:+2188305 | None:intergenic |
| GGATAAGAGAGTACATGAGA+AGG | 0.565832 | 5.1:+2188176 | None:intergenic |
| CCTAGACTCTCTATTGATTG+GGG | 0.565888 | 5.1:-2187356 | MS.gene21886:CDS |
| ACTATTATGCTCAAGATGGA+GGG | 0.566137 | 5.1:-2187133 | MS.gene21886:CDS |
| TTGAAGAGCGATGCGTTTAG+CGG | 0.568818 | 5.1:+2188210 | None:intergenic |
| ATGCTCAAGATGGAGGGGAG+TGG | 0.569680 | 5.1:-2187127 | MS.gene21886:CDS |
| TCTTGAGCATAATAGTCCAT+TGG | 0.570183 | 5.1:+2187140 | None:intergenic |
| GCAGACAACGGCTTCGACTG+CGG | 0.570442 | 5.1:+2187800 | None:intergenic |
| ATGGACTATTATGCTCAAGA+TGG | 0.571439 | 5.1:-2187137 | MS.gene21886:CDS |
| TTTAGGGTGTACTGCACCGA+AGG | 0.572206 | 5.1:-2187768 | MS.gene21886:CDS |
| ATCGCTCTTCAATTCCCTGA+AGG | 0.572586 | 5.1:-2188199 | MS.gene21886:CDS |
| TTCTTTGATAGAAACAGTGA+TGG | 0.575244 | 5.1:+2188375 | None:intergenic |
| TTTGAGGCTGAGATAGCACT+GGG | 0.575711 | 5.1:-2187302 | MS.gene21886:CDS |
| CTATTATGCTCAAGATGGAG+GGG | 0.577204 | 5.1:-2187132 | MS.gene21886:CDS |
| ATCAATACAACAAGCACCAT+AGG | 0.579949 | 5.1:+2188093 | None:intergenic |
| GCGAAGGAGGCAAGGAATTG+GGG | 0.585334 | 5.1:-2187551 | MS.gene21886:CDS |
| AAAAGCCTAGTCGTCCTGCT+AGG | 0.588814 | 5.1:-2187088 | MS.gene21886:CDS |
| TGGGATTGATACCTGGATGG+TGG | 0.589217 | 5.1:-2187283 | MS.gene21886:CDS |
| TAAAGGATTTGATTATACTG+TGG | 0.592986 | 5.1:-2187456 | MS.gene21886:CDS |
| GGGATTGATACCTGGATGGT+GGG | 0.594275 | 5.1:-2187282 | MS.gene21886:CDS |
| AGAGATTGGAAATGAAAATG+GGG | 0.600592 | 5.1:-2187481 | MS.gene21886:CDS |
| ATAGCACTGGGATTGATACC+TGG | 0.600649 | 5.1:-2187290 | MS.gene21886:CDS |
| ACCGAAGGTGGGGAAAGAGT+TGG | 0.600972 | 5.1:-2187753 | MS.gene21886:CDS |
| TGTTGCTCTAACGGTAGCTG+CGG | 0.603328 | 5.1:-2187200 | MS.gene21886:CDS |
| CCCCAATCAATAGAGAGTCT+AGG | 0.603832 | 5.1:+2187356 | None:intergenic |
| ATGTGGGTATGAAGGGGTCG+AGG | 0.608316 | 5.1:-2187592 | MS.gene21886:CDS |
| TTTGGAGGAATATGATCATG+TGG | 0.610687 | 5.1:-2187609 | MS.gene21886:CDS |
| GTGAGTTACACCGGCGAAGG+TGG | 0.617454 | 5.1:+2188138 | None:intergenic |
| AAAACTCAGATGCAGAAACA+AGG | 0.622881 | 5.1:-2187257 | MS.gene21886:CDS |
| TTAGGGACTTTGGGTAGACA+AGG | 0.624346 | 5.1:-2187521 | MS.gene21886:CDS |
| GAGATTGGAAATGAAAATGG+GGG | 0.625888 | 5.1:-2187480 | MS.gene21886:CDS |
| ACAGTGAGTTACACCGGCGA+AGG | 0.629851 | 5.1:+2188135 | None:intergenic |
| AACGTAACAGTGAGTTACAC+CGG | 0.640720 | 5.1:+2188129 | None:intergenic |
| TAGGGACTTTGGGTAGACAA+GGG | 0.641045 | 5.1:-2187520 | MS.gene21886:CDS |
| CGTTTATGATAGCGAATCCG+GGG | 0.643038 | 5.1:-2187673 | MS.gene21886:CDS |
| AAGAGCGATGCGTTTAGCGG+CGG | 0.644502 | 5.1:+2188213 | None:intergenic |
| CACTGGGATTGATACCTGGA+TGG | 0.644522 | 5.1:-2187286 | MS.gene21886:CDS |
| ATGATCATGTGGGTATGAAG+GGG | 0.649107 | 5.1:-2187598 | MS.gene21886:CDS |
| AAAATCTCACCAGCAGACAA+CGG | 0.661511 | 5.1:+2187788 | None:intergenic |
| TTAGGTATGACCCTTATGTG+GGG | 0.683162 | 5.1:-2187640 | MS.gene21886:CDS |
| TTGCTCTAACGGTAGCTGCG+GGG | 0.685362 | 5.1:-2187198 | MS.gene21886:CDS |
| AGGTGGTGAAGATATCAGAG+AGG | 0.733119 | 5.1:+2188155 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CAAACAAAGAGATTCTATAA+GGG | + | chr5.1:2188044-2188063 | None:intergenic | 25.0% |
| ! | TAAAGGATTTGATTATACTG+TGG | - | chr5.1:2187999-2188018 | MS.gene21886:CDS | 25.0% |
| ! | TAGGAAAATTTCAGTAACTT+CGG | - | chr5.1:2188386-2188405 | MS.gene21886:CDS | 25.0% |
| ! | TCAAACAAAGAGATTCTATA+AGG | + | chr5.1:2188045-2188064 | None:intergenic | 25.0% |
| ! | TCAACAAAAACATAAAGACA+AGG | + | chr5.1:2187472-2187491 | None:intergenic | 25.0% |
| !! | AAGTTGAATTCTAAGGATTT+AGG | - | chr5.1:2187529-2187548 | MS.gene21886:CDS | 25.0% |
| !! | AGTTGAATTCTAAGGATTTA+GGG | - | chr5.1:2187530-2187549 | MS.gene21886:CDS | 25.0% |
| !!! | TACTGTTAAGTTGAATTCTA+AGG | - | chr5.1:2187522-2187541 | MS.gene21886:CDS | 25.0% |
| AATTGGAGAAATTAGGGTAT+AGG | - | chr5.1:2187623-2187642 | MS.gene21886:CDS | 30.0% | |
| AGAGATTGGAAATGAAAATG+GGG | - | chr5.1:2187974-2187993 | MS.gene21886:CDS | 30.0% | |
| ATTGGAGAAATTAGGGTATA+GGG | - | chr5.1:2187624-2187643 | MS.gene21886:CDS | 30.0% | |
| GAGAGATTGGAAATGAAAAT+GGG | - | chr5.1:2187973-2187992 | MS.gene21886:CDS | 30.0% | |
| GCTGAGATTCTTATTCATTA+TGG | - | chr5.1:2187409-2187428 | MS.gene21886:CDS | 30.0% | |
| TTCTTTGATAGAAACAGTGA+TGG | + | chr5.1:2187083-2187102 | None:intergenic | 30.0% | |
| ! | ATTTAGGGTTTATGGGTAAA+AGG | - | chr5.1:2187545-2187564 | MS.gene21886:CDS | 30.0% |
| ! | GGAATTTTTGTCGAATCAAT+AGG | + | chr5.1:2187451-2187470 | None:intergenic | 30.0% |
| ! | TCTAAGGATTTAGGGTTTAT+GGG | - | chr5.1:2187538-2187557 | MS.gene21886:CDS | 30.0% |
| ! | TGGTTTTTGATGAATCGTTT+TGG | + | chr5.1:2187115-2187134 | None:intergenic | 30.0% |
| ! | TTCTAAGGATTTAGGGTTTA+TGG | - | chr5.1:2187537-2187556 | MS.gene21886:CDS | 30.0% |
| ! | TTTAGGGTTTATGGGTAAAA+GGG | - | chr5.1:2187546-2187565 | MS.gene21886:CDS | 30.0% |
| !! | AAATGCAATTTTGAAAGCGA+AGG | - | chr5.1:2187888-2187907 | MS.gene21886:CDS | 30.0% |
| !!! | ACTAGGCTTTTTCATATAAG+AGG | + | chr5.1:2188358-2188377 | None:intergenic | 30.0% |
| !!! | TTGATGAATCGTTTTGGTTT+TGG | + | chr5.1:2187109-2187128 | None:intergenic | 30.0% |
| AAAAACAACTTCCCCACATA+AGG | + | chr5.1:2187829-2187848 | None:intergenic | 35.0% | |
| AAAACAACTTCCCCACATAA+GGG | + | chr5.1:2187828-2187847 | None:intergenic | 35.0% | |
| AAAACTCAGATGCAGAAACA+AGG | - | chr5.1:2188198-2188217 | MS.gene21886:CDS | 35.0% | |
| AAGCCTGAATTGGAGAAATT+AGG | - | chr5.1:2187616-2187635 | MS.gene21886:CDS | 35.0% | |
| ACTATTATGCTCAAGATGGA+GGG | - | chr5.1:2188322-2188341 | MS.gene21886:CDS | 35.0% | |
| AGCCTGAATTGGAGAAATTA+GGG | - | chr5.1:2187617-2187636 | MS.gene21886:CDS | 35.0% | |
| ATATGATCATGTGGGTATGA+AGG | - | chr5.1:2187855-2187874 | MS.gene21886:CDS | 35.0% | |
| ATCAATACAACAAGCACCAT+AGG | + | chr5.1:2187365-2187384 | None:intergenic | 35.0% | |
| GAAATGAAAATGGGGGATAA+AGG | - | chr5.1:2187982-2188001 | MS.gene21886:CDS | 35.0% | |
| GAGATTGGAAATGAAAATGG+GGG | - | chr5.1:2187975-2187994 | MS.gene21886:CDS | 35.0% | |
| GGAGAGATTGGAAATGAAAA+TGG | - | chr5.1:2187972-2187991 | MS.gene21886:CDS | 35.0% | |
| GTAAAAGGGTTATTCTTGCT+GGG | - | chr5.1:2187560-2187579 | MS.gene21886:CDS | 35.0% | |
| TACCCTAATTTCTCCAATTC+AGG | + | chr5.1:2187622-2187641 | None:intergenic | 35.0% | |
| TATGATCATGTGGGTATGAA+GGG | - | chr5.1:2187856-2187875 | MS.gene21886:CDS | 35.0% | |
| TCCAATCTCTCCAAAATCTT+AGG | + | chr5.1:2187964-2187983 | None:intergenic | 35.0% | |
| TCTTGAGCATAATAGTCCAT+TGG | + | chr5.1:2188318-2188337 | None:intergenic | 35.0% | |
| TTGGAGGAATATGATCATGT+GGG | - | chr5.1:2187847-2187866 | MS.gene21886:CDS | 35.0% | |
| TTTAGGTATGACCCTTATGT+GGG | - | chr5.1:2187814-2187833 | MS.gene21886:CDS | 35.0% | |
| TTTGGAGGAATATGATCATG+TGG | - | chr5.1:2187846-2187865 | MS.gene21886:CDS | 35.0% | |
| ! | ACAAGGGAATCCTAAGATTT+TGG | - | chr5.1:2187951-2187970 | MS.gene21886:CDS | 35.0% |
| ! | ATGGACTATTATGCTCAAGA+TGG | - | chr5.1:2188318-2188337 | MS.gene21886:CDS | 35.0% |
| ! | CTCTTTGTTTGAAGATTCTG+TGG | - | chr5.1:2188053-2188072 | MS.gene21886:CDS | 35.0% |
| ! | GAAATTAGGGTATAGGGTTT+TGG | - | chr5.1:2187630-2187649 | MS.gene21886:CDS | 35.0% |
| ! | GTTACTGAAATTTTCCTAGC+AGG | + | chr5.1:2188384-2188403 | None:intergenic | 35.0% |
| ! | TCCTAGACTCTCTATTGATT+GGG | - | chr5.1:2188098-2188117 | MS.gene21886:CDS | 35.0% |
| ! | TTTTGGTGGAGATTATCCAA+TGG | - | chr5.1:2188299-2188318 | MS.gene21886:CDS | 35.0% |
| !! | TCCTAAGATTTTGGAGAGAT+TGG | - | chr5.1:2187960-2187979 | MS.gene21886:CDS | 35.0% |
| !! | TTATGTGGGGAAGTTGTTTT+TGG | - | chr5.1:2187828-2187847 | MS.gene21886:CDS | 35.0% |
| !! | TTTTAGGTATGACCCTTATG+TGG | - | chr5.1:2187813-2187832 | MS.gene21886:CDS | 35.0% |
| !!! | TGATAAGGAAGAGAGTGTTT+TGG | - | chr5.1:2187726-2187745 | MS.gene21886:CDS | 35.0% |
| AAAATCTCACCAGCAGACAA+CGG | + | chr5.1:2187670-2187689 | None:intergenic | 40.0% | |
| AACGCATTTGTGAACTTCGA+AGG | + | chr5.1:2187206-2187225 | None:intergenic | 40.0% | |
| AACGTAACAGTGAGTTACAC+CGG | + | chr5.1:2187329-2187348 | None:intergenic | 40.0% | |
| AAGAGCGATTGTTGCTCTAA+CGG | - | chr5.1:2188246-2188265 | MS.gene21886:CDS | 40.0% | |
| ACTTCGAAGGAGTAGTTTGA+TGG | + | chr5.1:2187193-2187212 | None:intergenic | 40.0% | |
| ATGATCATGTGGGTATGAAG+GGG | - | chr5.1:2187857-2187876 | MS.gene21886:CDS | 40.0% | |
| CAAGGTTGTGAAGATGTTAC+AGG | - | chr5.1:2188216-2188235 | MS.gene21886:CDS | 40.0% | |
| CTATTATGCTCAAGATGGAG+GGG | - | chr5.1:2188323-2188342 | MS.gene21886:CDS | 40.0% | |
| GACTATTATGCTCAAGATGG+AGG | - | chr5.1:2188321-2188340 | MS.gene21886:CDS | 40.0% | |
| GATTTCTCTGCTATTGCTCT+TGG | - | chr5.1:2187385-2187404 | MS.gene21886:CDS | 40.0% | |
| GGTAAAAGGGTTATTCTTGC+TGG | - | chr5.1:2187559-2187578 | MS.gene21886:CDS | 40.0% | |
| GTAACTCACTGTTACGTTCT+CGG | - | chr5.1:2187331-2187350 | MS.gene21886:CDS | 40.0% | |
| GTCCTAGACTCTCTATTGAT+TGG | - | chr5.1:2188097-2188116 | MS.gene21886:CDS | 40.0% | |
| TACCTAAAAGCCTTAATCCC+CGG | + | chr5.1:2187802-2187821 | None:intergenic | 40.0% | |
| TCGAAGTTCACAAATGCGTT+TGG | - | chr5.1:2187206-2187225 | MS.gene21886:CDS | 40.0% | |
| TGATGGAAGGATTGAGATTG+CGG | + | chr5.1:2187176-2187195 | None:intergenic | 40.0% | |
| TTAGGTATGACCCTTATGTG+GGG | - | chr5.1:2187815-2187834 | MS.gene21886:CDS | 40.0% | |
| TTGAAGATTCTGTGGATGCT+TGG | - | chr5.1:2188061-2188080 | MS.gene21886:CDS | 40.0% | |
| ! | AGCGTTTATGATAGCGAATC+CGG | - | chr5.1:2187780-2187799 | MS.gene21886:CDS | 40.0% |
| ! | CCTAGACTCTCTATTGATTG+GGG | - | chr5.1:2188099-2188118 | MS.gene21886:CDS | 40.0% |
| ! | GGACTATTCAGTTTGCTTCT+GGG | - | chr5.1:2187581-2187600 | MS.gene21886:CDS | 40.0% |
| ! | GGATAAGAGAGTACATGAGA+AGG | + | chr5.1:2187282-2187301 | None:intergenic | 40.0% |
| ! | TGAGGATTGAATCTGGGATT+TGG | + | chr5.1:2187135-2187154 | None:intergenic | 40.0% |
| ! | TGTCTGCTGGTGAGATTTTA+GGG | - | chr5.1:2187671-2187690 | MS.gene21886:CDS | 40.0% |
| ! | TTGTCTGCTGGTGAGATTTT+AGG | - | chr5.1:2187670-2187689 | MS.gene21886:CDS | 40.0% |
| !! | AGAAACAGTGATGGTGTTGT+TGG | + | chr5.1:2187074-2187093 | None:intergenic | 40.0% |
| !! | GAAACAGTGATGGTGTTGTT+GGG | + | chr5.1:2187073-2187092 | None:intergenic | 40.0% |
| !! | TCAGTTTGCTTCTGGGATTA+GGG | - | chr5.1:2187588-2187607 | MS.gene21886:CDS | 40.0% |
| !! | TGCAATTTTGAAAGCGAAGG+AGG | - | chr5.1:2187891-2187910 | MS.gene21886:CDS | 40.0% |
| !! | TTCAGTTTGCTTCTGGGATT+AGG | - | chr5.1:2187587-2187606 | MS.gene21886:CDS | 40.0% |
| !!! | ATGGTGTTGTTGGGTTTTTC+AGG | + | chr5.1:2187064-2187083 | None:intergenic | 40.0% |
| !!! | GAGTGTTTTGGTGTTTGTGT+CGG | - | chr5.1:2187738-2187757 | MS.gene21886:CDS | 40.0% |
| !!! | TTGGGTTTTTCAGGTTGTTC+TGG | + | chr5.1:2187055-2187074 | None:intergenic | 40.0% |
| ACCAAAATCTTCAGTCGCCT+CGG | + | chr5.1:2188286-2188305 | None:intergenic | 45.0% | |
| AGGTGGTGAAGATATCAGAG+AGG | + | chr5.1:2187303-2187322 | None:intergenic | 45.0% | |
| AGTACATGAGAAGGCCTTCA+GGG | + | chr5.1:2187273-2187292 | None:intergenic | 45.0% | |
| AGTGCTATCTCAGCCTCAAA+AGG | + | chr5.1:2188153-2188172 | None:intergenic | 45.0% | |
| ATCGCTCTTCAATTCCCTGA+AGG | - | chr5.1:2187256-2187275 | MS.gene21886:CDS | 45.0% | |
| CCCCAATCAATAGAGAGTCT+AGG | + | chr5.1:2188102-2188121 | None:intergenic | 45.0% | |
| CGAAGGAGTAGTTTGATGGA+AGG | + | chr5.1:2187189-2187208 | None:intergenic | 45.0% | |
| GGGGAAAGAGTTGGATGATA+AGG | - | chr5.1:2187711-2187730 | MS.gene21886:CDS | 45.0% | |
| GGTCGTTGAGGATTGAATCT+GGG | + | chr5.1:2187141-2187160 | None:intergenic | 45.0% | |
| GTTCTCGGTGATGTTACCTA+TGG | - | chr5.1:2187346-2187365 | MS.gene21886:CDS | 45.0% | |
| TAGGGACTTTGGGTAGACAA+GGG | - | chr5.1:2187935-2187954 | MS.gene21886:CDS | 45.0% | |
| TTAGGGACTTTGGGTAGACA+AGG | - | chr5.1:2187934-2187953 | MS.gene21886:CDS | 45.0% | |
| TTGAAGAGCGATGCGTTTAG+CGG | + | chr5.1:2187248-2187267 | None:intergenic | 45.0% | |
| ! | ATAGCACTGGGATTGATACC+TGG | - | chr5.1:2188165-2188184 | MS.gene21886:CDS | 45.0% |
| ! | ATCCGGGGATTAAGGCTTTT+AGG | - | chr5.1:2187797-2187816 | MS.gene21886:CDS | 45.0% |
| ! | ATTTTCCTAGCAGGACGACT+AGG | + | chr5.1:2188375-2188394 | None:intergenic | 45.0% |
| ! | CGTTTATGATAGCGAATCCG+GGG | - | chr5.1:2187782-2187801 | MS.gene21886:CDS | 45.0% |
| ! | GCGTTTATGATAGCGAATCC+GGG | - | chr5.1:2187781-2187800 | MS.gene21886:CDS | 45.0% |
| ! | GGGACTATTCAGTTTGCTTC+TGG | - | chr5.1:2187580-2187599 | MS.gene21886:CDS | 45.0% |
| ! | TTTGAGGCTGAGATAGCACT+GGG | - | chr5.1:2188153-2188172 | MS.gene21886:CDS | 45.0% |
| !! | ACCAGTTCTGACTCCTTTTG+AGG | - | chr5.1:2188137-2188156 | MS.gene21886:CDS | 45.0% |
| !! | TTTTGAAAGCGAAGGAGGCA+AGG | - | chr5.1:2187896-2187915 | MS.gene21886:CDS | 45.0% |
| !!! | AGGAATTGGGGCGTTGTTTT+AGG | - | chr5.1:2187916-2187935 | MS.gene21886:CDS | 45.0% |
| !!! | GGAATTGGGGCGTTGTTTTA+GGG | - | chr5.1:2187917-2187936 | MS.gene21886:CDS | 45.0% |
| !!! | GGCGTTGTTTTAGGGACTTT+GGG | - | chr5.1:2187925-2187944 | MS.gene21886:CDS | 45.0% |
| !!! | GTTTTGGTGTTTGTGTCGGA+TGG | - | chr5.1:2187742-2187761 | MS.gene21886:CDS | 45.0% |
| !!! | TGTGGGGAAGTTGTTTTTGG+AGG | - | chr5.1:2187831-2187850 | MS.gene21886:CDS | 45.0% |
| !!! | TTTTGAGGCTGAGATAGCAC+TGG | - | chr5.1:2188152-2188171 | MS.gene21886:CDS | 45.0% |
| AAAAGCCTAGTCGTCCTGCT+AGG | - | chr5.1:2188367-2188386 | MS.gene21886:CDS | 50.0% | |
| AAGCGAAGGAGGCAAGGAAT+TGG | - | chr5.1:2187902-2187921 | MS.gene21886:CDS | 50.0% | |
| AGCGAAGGAGGCAAGGAATT+GGG | - | chr5.1:2187903-2187922 | MS.gene21886:CDS | 50.0% | |
| CACTGGGATTGATACCTGGA+TGG | - | chr5.1:2188169-2188188 | MS.gene21886:CDS | 50.0% | |
| GAGTACATGAGAAGGCCTTC+AGG | + | chr5.1:2187274-2187293 | None:intergenic | 50.0% | |
| GATAGCGAATCCGGGGATTA+AGG | - | chr5.1:2187789-2187808 | MS.gene21886:CDS | 50.0% | |
| GATATCTTCACCACCTTCGC+CGG | - | chr5.1:2187307-2187326 | MS.gene21886:CDS | 50.0% | |
| GCCTCAAAAGGAGTCAGAAC+TGG | + | chr5.1:2188141-2188160 | None:intergenic | 50.0% | |
| GGGATTGATACCTGGATGGT+GGG | - | chr5.1:2188173-2188192 | MS.gene21886:CDS | 50.0% | |
| GGGTCGTTGAGGATTGAATC+TGG | + | chr5.1:2187142-2187161 | None:intergenic | 50.0% | |
| TAGGGCTGTGAAGCCTGAAT+TGG | - | chr5.1:2187606-2187625 | MS.gene21886:CDS | 50.0% | |
| TCCAACTCTTTCCCCACCTT+CGG | + | chr5.1:2187706-2187725 | None:intergenic | 50.0% | |
| TGGGATTGATACCTGGATGG+TGG | - | chr5.1:2188172-2188191 | MS.gene21886:CDS | 50.0% | |
| TGGTGTTTGTGTCGGATGGA+AGG | - | chr5.1:2187746-2187765 | MS.gene21886:CDS | 50.0% | |
| TGTTGCTCTAACGGTAGCTG+CGG | - | chr5.1:2188255-2188274 | MS.gene21886:CDS | 50.0% | |
| ! | AGATTGCGGCGTTGAGAAGT+GGG | + | chr5.1:2187162-2187181 | None:intergenic | 50.0% |
| ! | GAGGCGACTGAAGATTTTGG+TGG | - | chr5.1:2188285-2188304 | MS.gene21886:CDS | 50.0% |
| ! | TGAGTTTTCTCCCACCATCC+AGG | + | chr5.1:2188186-2188205 | None:intergenic | 50.0% |
| ! | TTTAGGGTGTACTGCACCGA+AGG | - | chr5.1:2187687-2187706 | MS.gene21886:CDS | 50.0% |
| !!! | GGGCGTTGTTTTAGGGACTT+TGG | - | chr5.1:2187924-2187943 | MS.gene21886:CDS | 50.0% |
| AAGAGCGATGCGTTTAGCGG+CGG | + | chr5.1:2187245-2187264 | None:intergenic | 55.0% | |
| ACAGTGAGTTACACCGGCGA+AGG | + | chr5.1:2187323-2187342 | None:intergenic | 55.0% | |
| ACCGAAGGTGGGGAAAGAGT+TGG | - | chr5.1:2187702-2187721 | MS.gene21886:CDS | 55.0% | |
| ATGCTCAAGATGGAGGGGAG+TGG | - | chr5.1:2188328-2188347 | MS.gene21886:CDS | 55.0% | |
| ATGTGGGTATGAAGGGGTCG+AGG | - | chr5.1:2187863-2187882 | MS.gene21886:CDS | 55.0% | |
| CGTTGAGAAGTGGGTCGTTG+AGG | + | chr5.1:2187153-2187172 | None:intergenic | 55.0% | |
| GCGAAGGAGGCAAGGAATTG+GGG | - | chr5.1:2187904-2187923 | MS.gene21886:CDS | 55.0% | |
| GTTGCTCTAACGGTAGCTGC+GGG | - | chr5.1:2188256-2188275 | MS.gene21886:CDS | 55.0% | |
| TTGCTCTAACGGTAGCTGCG+GGG | - | chr5.1:2188257-2188276 | MS.gene21886:CDS | 55.0% | |
| ! | GAGATTGCGGCGTTGAGAAG+TGG | + | chr5.1:2187163-2187182 | None:intergenic | 55.0% |
| ! | GCCGAGGCGACTGAAGATTT+TGG | - | chr5.1:2188282-2188301 | MS.gene21886:CDS | 55.0% |
| AGGGTGTACTGCACCGAAGG+TGG | - | chr5.1:2187690-2187709 | MS.gene21886:CDS | 60.0% | |
| CAGTCGAAGCCGTTGTCTGC+TGG | - | chr5.1:2187658-2187677 | MS.gene21886:CDS | 60.0% | |
| GCAGACAACGGCTTCGACTG+CGG | + | chr5.1:2187658-2187677 | None:intergenic | 60.0% | |
| GGGTGTACTGCACCGAAGGT+GGG | - | chr5.1:2187691-2187710 | MS.gene21886:CDS | 60.0% | |
| GGTGTACTGCACCGAAGGTG+GGG | - | chr5.1:2187692-2187711 | MS.gene21886:CDS | 60.0% | |
| GTGAGTTACACCGGCGAAGG+TGG | + | chr5.1:2187320-2187339 | None:intergenic | 60.0% | |
| TGCGTTTAGCGGCGGTGGAA+CGG | + | chr5.1:2187237-2187256 | None:intergenic | 60.0% | |
| AGCGATGCGTTTAGCGGCGG+TGG | + | chr5.1:2187242-2187261 | None:intergenic | 65.0% | |
| CGGTAGCTGCGGGGATGCCG+AGG | - | chr5.1:2188266-2188285 | MS.gene21886:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 2187043 | 2188434 | 2187043 | ID=MS.gene21886 |
| chr5.1 | mRNA | 2187043 | 2188434 | 2187043 | ID=MS.gene21886.t1;Parent=MS.gene21886 |
| chr5.1 | exon | 2187043 | 2188434 | 2187043 | ID=MS.gene21886.t1.exon1;Parent=MS.gene21886.t1 |
| chr5.1 | CDS | 2187043 | 2188434 | 2187043 | ID=cds.MS.gene21886.t1;Parent=MS.gene21886.t1 |
| Gene Sequence |
| Protein sequence |
