AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24651


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24651.t1 MTR_7g104020 100.000 168 0 0 1 168 1 168 2.79e-118 331
MS.gene24651.t1 MTR_7g104020 98.246 171 0 1 1 168 1 171 3.32e-116 326
MS.gene24651.t1 MTR_2g085025 69.032 155 46 1 14 166 20 174 6.12e-63 191
MS.gene24651.t1 MTR_1g492930 59.494 158 61 1 14 168 4 161 6.71e-59 181
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24651.t1 AT2G36410 77.564 156 34 1 10 165 38 192 1.68e-84 247
MS.gene24651.t1 AT2G36410 76.101 159 34 2 10 165 38 195 2.67e-82 241
MS.gene24651.t1 AT3G09980 82.911 158 27 0 9 166 21 178 1.76e-81 239
MS.gene24651.t1 AT3G52920 80.137 146 29 0 19 164 17 162 4.51e-81 238
MS.gene24651.t1 AT3G52920 78.523 149 29 1 19 164 17 165 7.78e-79 232
MS.gene24651.t1 AT5G03660 72.785 158 43 0 6 163 12 169 1.21e-65 198
MS.gene24651.t1 AT2G27740 60.526 152 58 1 14 163 19 170 2.87e-62 190
MS.gene24651.t1 AT2G36410 76.415 106 24 1 10 115 38 142 2.98e-54 169
MS.gene24651.t1 AT5G03660 70.504 139 41 0 6 144 12 150 8.90e-54 167
MS.gene24651.t1 AT3G52900 53.659 164 72 2 6 166 2 164 1.62e-52 165
MS.gene24651.t1 AT5G03660 62.025 158 38 1 6 163 12 147 9.39e-47 150
MS.gene24651.t1 AT2G36355 50.331 151 75 0 16 166 5 155 5.15e-45 145
MS.gene24651.t1 AT2G27740 57.692 104 42 1 14 115 19 122 8.57e-39 129
MS.gene24651.t1 AT2G36355 58.621 87 36 0 80 166 27 113 8.20e-31 108
MS.gene24651.t1 AT2G36355 58.333 84 35 0 83 166 1 84 5.65e-30 105
MS.gene24651.t1 AT2G36355 46.000 100 54 0 16 115 5 104 1.05e-22 88.2

Find 31 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGTTGGTTGTACAGAATTC+AGG 0.260998 7.2:+8760001 MS.gene24651:CDS
ACAGCTAATTACTAAATTAA+TGG 0.284229 7.2:+8761189 MS.gene24651:CDS
AAGCTTTCAATGAAAAGAAT+AGG 0.336763 7.2:+8761158 MS.gene24651:CDS
AGTGAAACTTCTTTCCTCAT+TGG 0.356625 7.2:-8760928 None:intergenic
TAACCTAGCTCACCTTCTTC+TGG 0.386647 7.2:-8761008 None:intergenic
AGCTTTCAATGAAAAGAATA+GGG 0.424472 7.2:+8761159 MS.gene24651:CDS
CTTTCCTCATTGGATCTGCA+AGG 0.450818 7.2:-8760918 None:intergenic
AGAAATTGAAAAGAAGAAGA+TGG 0.462319 7.2:+8760108 MS.gene24651:CDS
CTGAATTCTGTACAACCAAC+TGG 0.468188 7.2:-8760000 None:intergenic
AAGATTGAGGATGAAGAAGC+TGG 0.477057 7.2:+8761930 MS.gene24651:CDS
GCTAAATCATTGCATTTGCA+TGG 0.478899 7.2:-8761977 None:intergenic
GACAAGGTTCAGTTTCAGTT+GGG 0.484764 7.2:+8760139 MS.gene24651:CDS
TTTCCTCATTGGATCTGCAA+GGG 0.489503 7.2:-8760917 None:intergenic
AGACAAGGTTCAGTTTCAGT+TGG 0.495018 7.2:+8760138 MS.gene24651:CDS
AGTTGGTTGTACAGAATTCA+GGG 0.496303 7.2:+8760002 MS.gene24651:CDS
TGGTCATACCTGCCAGAAGA+AGG 0.536387 7.2:+8760996 MS.gene24651:CDS
CTTTAGCAGTCACTTGTCAA+AGG 0.542571 7.2:+8760033 MS.gene24651:CDS
CTAGCTCACCTTCTTCTGGC+AGG 0.555949 7.2:-8761004 None:intergenic
TTCTGGCAGGTATGACCAAG+TGG 0.569790 7.2:-8760991 None:intergenic
TGTTGATGTGATTAGCTGGT+GGG 0.572662 7.2:+8761898 MS.gene24651:intron
GTTGATGTGATTAGCTGGTG+GGG 0.573033 7.2:+8761899 MS.gene24651:intron
AGGAAGATGAAGAGATGTCA+AGG 0.586395 7.2:+8760053 MS.gene24651:CDS
AACAAAGAATTAAAGCCACT+TGG 0.593321 7.2:+8760976 MS.gene24651:CDS
TTGATGTGATTAGCTGGTGG+GGG 0.596450 7.2:+8761900 MS.gene24651:intron
GCTAATTACTAAATTAATGG+AGG 0.597346 7.2:+8761192 MS.gene24651:CDS
CAAAATATAAACCTCACGAA+TGG 0.615296 7.2:-8760194 None:intergenic
ACGTCTTGCTACCATTCGTG+AGG 0.618633 7.2:+8760183 MS.gene24651:CDS
GAAGATGGAAGTTAGAGACA+AGG 0.621610 7.2:+8760123 MS.gene24651:CDS
AAGCCCTTGCAGATCCAATG+AGG 0.655089 7.2:+8760914 MS.gene24651:CDS
AAGCAACAGTAACAACCAGT+TGG 0.659075 7.2:+8759985 MS.gene24651:CDS
ATTGAGGATGAAGAAGCTGG+AGG 0.703230 7.2:+8761933 MS.gene24651:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGGAAAAAAAAAAATAA+TGG - chr7.2:8760805-8760824 None:intergenic 10.0%
!! AAATCTAAGAAGAAAAAAAA+AGG - chr7.2:8760222-8760241 None:intergenic 15.0%
!! AACATAAGTACAAAAATTTA+AGG + chr7.2:8760344-8760363 MS.gene24651:intron 15.0%
!! AATTCAATTTCAAATTTCAA+TGG - chr7.2:8761098-8761117 None:intergenic 15.0%
!! GAATATACATAAATATCTTT+AGG + chr7.2:8761796-8761815 MS.gene24651:intron 15.0%
!! TGTAATAATTTGATTAATTG+AGG + chr7.2:8760546-8760565 MS.gene24651:intron 15.0%
!!! TAAATTTTTGTACTTATGTT+TGG - chr7.2:8760344-8760363 None:intergenic 15.0%
!!! TTTTTTTCCAAAAATGAAAA+GGG - chr7.2:8760278-8760297 None:intergenic 15.0%
!! AAAAAAAAAAATAATGGCTG+AGG - chr7.2:8760799-8760818 None:intergenic 20.0%
!! ACAGCTAATTACTAAATTAA+TGG + chr7.2:8761189-8761208 MS.gene24651:CDS 20.0%
!! ATACATATTACAAATGAGAA+TGG + chr7.2:8761647-8761666 MS.gene24651:intron 20.0%
!!! AAACTGTTAATTAGTGTTTT+TGG + chr7.2:8761048-8761067 MS.gene24651:intron 20.0%
!!! AACAAATTTTAGTTTCAAGT+TGG + chr7.2:8761387-8761406 MS.gene24651:intron 20.0%
!!! ATAAAATTGATTTTGACATG+AGG - chr7.2:8761728-8761747 None:intergenic 20.0%
!!! CTTTTTTTCCAAAAATGAAA+AGG - chr7.2:8760279-8760298 None:intergenic 20.0%
!!! TGATTTCGTAAAATTGATTT+TGG + chr7.2:8761298-8761317 MS.gene24651:intron 20.0%
!!! TTGAATTTTTGATACGTTAT+AGG + chr7.2:8761111-8761130 MS.gene24651:intron 20.0%
! AAATTTAAGGATTCAATACG+AGG + chr7.2:8760357-8760376 MS.gene24651:intron 25.0%
! AAGCTTTCAATGAAAAGAAT+AGG + chr7.2:8761158-8761177 MS.gene24651:CDS 25.0%
! AATCACATCAACAATTTAAG+AGG - chr7.2:8761891-8761910 None:intergenic 25.0%
! AGAAATTGAAAAGAAGAAGA+TGG + chr7.2:8760108-8760127 MS.gene24651:CDS 25.0%
! AGCTTTCAATGAAAAGAATA+GGG + chr7.2:8761159-8761178 MS.gene24651:CDS 25.0%
! ATCAACATGCTTATGAAAAA+AGG - chr7.2:8760822-8760841 None:intergenic 25.0%
! GCTAATTACTAAATTAATGG+AGG + chr7.2:8761192-8761211 MS.gene24651:CDS 25.0%
! TAAATTTGAGTGTAGAAGTT+AGG - chr7.2:8761537-8761556 None:intergenic 25.0%
! TGAGGGAAAAAAAAAAAAAC+TGG - chr7.2:8761680-8761699 None:intergenic 25.0%
!! ATTTCAGTTTTACTCAAACT+TGG + chr7.2:8760249-8760268 MS.gene24651:intron 25.0%
!! TATGTTATTTGAGGAATATC+AGG + chr7.2:8760885-8760904 MS.gene24651:intron 25.0%
!! TGCAAACTTTATGTTATTTG+AGG + chr7.2:8760876-8760895 MS.gene24651:intron 25.0%
AAATCAATTCTACTCTACCA+AGG - chr7.2:8761458-8761477 None:intergenic 30.0%
AAATTGTTGATGTGATTAGC+TGG + chr7.2:8761894-8761913 MS.gene24651:intron 30.0%
AACAAAGAATTAAAGCCACT+TGG + chr7.2:8760976-8760995 MS.gene24651:CDS 30.0%
CAAAATATAAACCTCACGAA+TGG - chr7.2:8760197-8760216 None:intergenic 30.0%
GATCATTCACAAAAACGATT+AGG - chr7.2:8761231-8761250 None:intergenic 30.0%
GTAATATGTATTTGCTTCTG+CGG - chr7.2:8761638-8761657 None:intergenic 30.0%
GTATATTCAATGTGTGTGTT+TGG - chr7.2:8761784-8761803 None:intergenic 30.0%
TCAATTCAATCAACCTTCTT+TGG + chr7.2:8761430-8761449 MS.gene24651:intron 30.0%
TGGCTGAGGATATTATTTAA+AGG - chr7.2:8760785-8760804 None:intergenic 30.0%
! CTTTTCATTGAAAGCTTCAA+GGG - chr7.2:8761155-8761174 None:intergenic 30.0%
! TCTTTTCATTGAAAGCTTCA+AGG - chr7.2:8761156-8761175 None:intergenic 30.0%
! TTGGAAACCCTTTTCATTTT+TGG + chr7.2:8760268-8760287 MS.gene24651:intron 30.0%
!! AAATTGATTTTGACATGAGG+AGG - chr7.2:8761725-8761744 None:intergenic 30.0%
ACTTGATTATACCCGATCTA+TGG + chr7.2:8760588-8760607 MS.gene24651:intron 35.0%
AGTGAAACTTCTTTCCTCAT+TGG - chr7.2:8760931-8760950 None:intergenic 35.0%
AGTTGGTTGTACAGAATTCA+GGG + chr7.2:8760002-8760021 MS.gene24651:CDS 35.0%
ATCTTTAGGCTGATACATGT+GGG + chr7.2:8761810-8761829 MS.gene24651:intron 35.0%
GAAAACTCTTATAATTCCCC+TGG + chr7.2:8760700-8760719 MS.gene24651:intron 35.0%
GTAGTTGTTATGTTGGAATC+TGG + chr7.2:8760382-8760401 MS.gene24651:intron 35.0%
TATCTTTAGGCTGATACATG+TGG + chr7.2:8761809-8761828 MS.gene24651:intron 35.0%
TTCTGACAAGAACAAATCCA+GGG - chr7.2:8760720-8760739 None:intergenic 35.0%
TTTCTGACAAGAACAAATCC+AGG - chr7.2:8760721-8760740 None:intergenic 35.0%
! AATGTGTGTGTTTGGTTTCT+CGG - chr7.2:8761776-8761795 None:intergenic 35.0%
! GTCTGCTCTATCAACTTTTA+GGG + chr7.2:8760075-8760094 MS.gene24651:CDS 35.0%
! TCTAGAACAGAACCATAGAT+CGG - chr7.2:8760603-8760622 None:intergenic 35.0%
AAGATTGAGGATGAAGAAGC+TGG + chr7.2:8761930-8761949 MS.gene24651:CDS 40.0%
AAGCAACAGTAACAACCAGT+TGG + chr7.2:8759985-8760004 MS.gene24651:CDS 40.0%
AGACAAGGTTCAGTTTCAGT+TGG + chr7.2:8760138-8760157 MS.gene24651:CDS 40.0%
AGGAAGATGAAGAGATGTCA+AGG + chr7.2:8760053-8760072 MS.gene24651:CDS 40.0%
CAGTTGGTTGTACAGAATTC+AGG + chr7.2:8760001-8760020 MS.gene24651:CDS 40.0%
CGAGGAAGTAGTTGTTATGT+TGG + chr7.2:8760375-8760394 MS.gene24651:intron 40.0%
CTAGAACAGAACCATAGATC+GGG - chr7.2:8760602-8760621 None:intergenic 40.0%
CTGAATTCTGTACAACCAAC+TGG - chr7.2:8760003-8760022 None:intergenic 40.0%
CTTTAGCAGTCACTTGTCAA+AGG + chr7.2:8760033-8760052 MS.gene24651:CDS 40.0%
GAAGATGGAAGTTAGAGACA+AGG + chr7.2:8760123-8760142 MS.gene24651:CDS 40.0%
GACAAGGTTCAGTTTCAGTT+GGG + chr7.2:8760139-8760158 MS.gene24651:CDS 40.0%
TCACATCAGAACTACACACT+AGG - chr7.2:8761256-8761275 None:intergenic 40.0%
TCTGACAAGAACAAATCCAG+GGG - chr7.2:8760719-8760738 None:intergenic 40.0%
TCTGGTGAACAAGAGTTTGT+TGG + chr7.2:8760400-8760419 MS.gene24651:intron 40.0%
TCTTTAGGCTGATACATGTG+GGG + chr7.2:8761811-8761830 MS.gene24651:intron 40.0%
TGTCCACTTGAGAAACATGA+GGG - chr7.2:8761697-8761716 None:intergenic 40.0%
TTGTCCACTTGAGAAACATG+AGG - chr7.2:8761698-8761717 None:intergenic 40.0%
TTTCCCTCATGTTTCTCAAG+TGG + chr7.2:8761691-8761710 MS.gene24651:intron 40.0%
TTTCCTCATTGGATCTGCAA+GGG - chr7.2:8760920-8760939 None:intergenic 40.0%
! ATCAACCTTCTTTGGTTCCT+TGG + chr7.2:8761438-8761457 MS.gene24651:intron 40.0%
! GGTCTGCTCTATCAACTTTT+AGG + chr7.2:8760074-8760093 MS.gene24651:CDS 40.0%
! TGTTGATGTGATTAGCTGGT+GGG + chr7.2:8761898-8761917 MS.gene24651:intron 40.0%
! TTGTTGATGTGATTAGCTGG+TGG + chr7.2:8761897-8761916 MS.gene24651:intron 40.0%
ATTGAGGATGAAGAAGCTGG+AGG + chr7.2:8761933-8761952 MS.gene24651:CDS 45.0%
CATCAGAACTACACACTAGG+AGG - chr7.2:8761253-8761272 None:intergenic 45.0%
CTCTACCAAGGAACCAAAGA+AGG - chr7.2:8761446-8761465 None:intergenic 45.0%
CTTTCCTCATTGGATCTGCA+AGG - chr7.2:8760921-8760940 None:intergenic 45.0%
TAACCTAGCTCACCTTCTTC+TGG - chr7.2:8761011-8761030 None:intergenic 45.0%
TGGGGGAAAGTGAAAGATTG+AGG + chr7.2:8761917-8761936 MS.gene24651:CDS 45.0%
! GTTGATGTGATTAGCTGGTG+GGG + chr7.2:8761899-8761918 MS.gene24651:intron 45.0%
! TTGATGTGATTAGCTGGTGG+GGG + chr7.2:8761900-8761919 MS.gene24651:intron 45.0%
AAGCCCTTGCAGATCCAATG+AGG + chr7.2:8760914-8760933 MS.gene24651:CDS 50.0%
ACGTCTTGCTACCATTCGTG+AGG + chr7.2:8760183-8760202 MS.gene24651:CDS 50.0%
TGGTCATACCTGCCAGAAGA+AGG + chr7.2:8760996-8761015 MS.gene24651:CDS 50.0%
TTCTGGCAGGTATGACCAAG+TGG - chr7.2:8760994-8761013 None:intergenic 50.0%
CTAGCTCACCTTCTTCTGGC+AGG - chr7.2:8761007-8761026 None:intergenic 55.0%
CTGCCAGAAGAAGGTGAGCT+AGG + chr7.2:8761005-8761024 MS.gene24651:intron 55.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 8759971 8761993 8759971 ID=MS.gene24651
chr7.2 mRNA 8759971 8761993 8759971 ID=MS.gene24651.t1;Parent=MS.gene24651
chr7.2 exon 8759971 8760204 8759971 ID=MS.gene24651.t1.exon1;Parent=MS.gene24651.t1
chr7.2 CDS 8759971 8760204 8759971 ID=cds.MS.gene24651.t1;Parent=MS.gene24651.t1
chr7.2 exon 8760907 8761017 8760907 ID=MS.gene24651.t1.exon2;Parent=MS.gene24651.t1
chr7.2 CDS 8760907 8761017 8760907 ID=cds.MS.gene24651.t1;Parent=MS.gene24651.t1
chr7.2 exon 8761133 8761213 8761133 ID=MS.gene24651.t1.exon3;Parent=MS.gene24651.t1
chr7.2 CDS 8761133 8761213 8761133 ID=cds.MS.gene24651.t1;Parent=MS.gene24651.t1
chr7.2 exon 8761913 8761993 8761913 ID=MS.gene24651.t1.exon4;Parent=MS.gene24651.t1
chr7.2 CDS 8761913 8761993 8761913 ID=cds.MS.gene24651.t1;Parent=MS.gene24651.t1
Gene Sequence

>MS.gene24651

ATGAGTGAACAACAAAGCAACAGTAACAACCAGTTGGTTGTACAGAATTCAGGGAGTTTAAGCTTTAGCAGTCACTTGTCAAAGGAAGATGAAGAGATGTCAAGGTCTGCTCTATCAACTTTTAGGGCTAAAGAAGAAGAAATTGAAAAGAAGAAGATGGAAGTTAGAGACAAGGTTCAGTTTCAGTTGGGTCGTGTTGAAGAAGAAACTAAACGTCTTGCTACCATTCGTGAGGTTTATATTTTGAACCTTTTTTTTTCTTCTTAGATTTATCTTGAATTTCAGTTTTACTCAAACTTGGAAACCCTTTTCATTTTTGGAAAAAAAGTTTTTTTTTTTTTTTTAAGAAATTTTGAAAAAAGTAAATTGACCAAACATAAGTACAAAAATTTAAGGATTCAATACGAGGAAGTAGTTGTTATGTTGGAATCTGGTGAACAAGAGTTTGTTGGAATGAATTAAATTAAATTAAATTTTATTTTATTTTTTTAATTGAACTATTTATTTATTTTTGCAAAAATAACAAGTTACTTTGTCTTTTTCTTGCATGTTATTCTTAATAAATCAAGTTTTGTTGTAATAATTTGATTAATTGAGGCTTGTAGAACATGTAGAACACTTGATTATACCCGATCTATGGTTCTGTTCTAGAAACAGAGTGAATTTGCATTAAAAAATAATTATATTTTTCTTTTCTCTTGTGTTTTATGAGAAAGTAATATAAAATTTGAAAACTCTTATAATTCCCCTGGATTTGTTCTTGTCAGAAAGTAGTATTAATTAATATATGAAACAAATAATTAATAATTTGCCTTTAAATAATATCCTCAGCCATTATTTTTTTTTTTCCTTTTTTCATAAGCATGTTGATATTGATATTGATATTGATATGTAATTGAACAAATTGCAAACTTTATGTTATTTGAGGAATATCAGGAGCTTGAAGCCCTTGCAGATCCAATGAGGAAAGAAGTTTCACTTGTTAGAAAGAGAATTGATTCAGTAAACAAAGAATTAAAGCCACTTGGTCATACCTGCCAGAAGAAGGTGAGCTAGGTTATTTTTAAATTCATAGAGAAACTGTTAATTAGTGTTTTTGGTCTAATGATATTTTATTAGTGTCACCATTGAAATTTGAAATTGAATTTTTGATACGTTATAGGAGAAAGAATACAAAGATGCCCTTGAAGCTTTCAATGAAAAGAATAGGGAAAAAGTACAGCTAATTACTAAATTAATGGAGGTTAGTCTCAAATTCCTAATCGTTTTTGTGAATGATCCTCCTAGTGTGTAGTTCTGATGTGATCAAAATTGAATTTGAATGTATTGATTTCGTAAAATTGATTTTGGTTAAAAGTTAGTTGATTGTGAAATGATTTGTATGATTATTTATATTCACATCAAAGTGAGTTCACAAACAAATTTTAGTTTCAAGTTGGTATGAACTCTAAAGAAAAGATCAATTCAATCAACCTTCTTTGGTTCCTTGGTAGAGTAGAATTGATTTTAACTTTAAAATTGATTCTAACTTGAAACTATAACTTGTAGTTATTTTATTCTACACCTAACTTCTACACTCAAATTTATTGTTCAACTCACACGTGCACAAATATATGCGAATATAAATTAGTGAGAATCAATTCACTCAAAAACAATTTTTATCACCGCAGAAGCAAATACATATTACAAATGAGAATGGATTGACTCCAGTTTTTTTTTTTTTCCCTCATGTTTCTCAAGTGGACAATTTTCCTCCTCATGTCAAAATCAATTTTATGTTTATAATCATTGTTAGCTGTCTACCGAGAAACCAAACACACACATTGAATATACATAAATATCTTTAGGCTGATACATGTGGGGTTTTATGTTCTTAATTTTATTGATTGATGTTGTTGCTACTAATTATTAGTAATACCTCTTAAATTGTTGATGTGATTAGCTGGTGGGGGAAAGTGAAAGATTGAGGATGAAGAAGCTGGAGGAGCTGAGTAAGAACATAGATTCCATGCAAATGCAATGA

Protein sequence

>MS.gene24651.t1

MSEQQSNSNNQLVVQNSGSLSFSSHLSKEDEEMSRSALSTFRAKEEEIEKKKMEVRDKVQFQLGRVEEETKRLATIREELEALADPMRKEVSLVRKRIDSVNKELKPLGHTCQKKEKEYKDALEAFNEKNREKVQLITKLMELVGESERLRMKKLEELSKNIDSMQMQ