AlfalfaGEDB | MS.gene27084

M. sativa cultivar XinJiangDaYe / MS.gene27084

KEGG: K09487
GO: GO:0000166 GO:0003674 GO:0005488 GO:0005515 GO:0005524 GO:0006457 GO:0008144 GO:0008150 GO:0009987 GO:0017076 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0051082 GO:0097159 GO:0097367 GO:1901265 GO:1901363
PFAM: PF00183.21 PF02518.29

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27084.t1	XP_003589505.1	99.5	781	4	0	1	781	1	781	0.00E+00	1404.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27084.t1	P35016	87.9	782	94	1	1	781	1	782	0.0e+00	1247.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27084.t1	G7I7Q4	99.5	781	4	0	1	781	1	781	0.0e+00	1404.8

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene27084	0.813478	2.51E-51	-1.69E-46
MS.gene049254	MS.gene27084	0.833077	6.56E-56	-1.69E-46
MS.gene049264	MS.gene27084	0.805663	1.20E-49	-1.69E-46
MS.gene049501	MS.gene27084	0.806066	9.85E-50	-1.69E-46
MS.gene049567	MS.gene27084	0.825621	4.24E-54	-1.69E-46
MS.gene050719	MS.gene27084	0.821963	3.05E-53	-1.69E-46
MS.gene050721	MS.gene27084	0.819942	8.91E-53	-1.69E-46
MS.gene050724	MS.gene27084	0.805276	1.44E-49	-1.69E-46
MS.gene050780	MS.gene27084	0.843932	1.03E-58	-1.69E-46
MS.gene051247	MS.gene27084	0.842616	2.32E-58	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene020833	MS.gene27084	PPI
MS.gene020834	MS.gene27084	PPI
MS.gene020886	MS.gene27084	PPI
MS.gene026627	MS.gene27084	PPI
MS.gene026628	MS.gene27084	PPI
MS.gene029690	MS.gene27084	PPI
MS.gene040499	MS.gene27084	PPI
MS.gene061126	MS.gene27084	PPI
MS.gene27084	MS.gene30654	PPI
MS.gene27084	MS.gene006231	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MS.gene27084.t1	MTR_1g025430	99.511	818	4	0	1	818	1	818	1644
MS.gene27084.t1	MTR_6g452990	49.508	711	317	7	75	780	2	675	648
MS.gene27084.t1	MTR_1g099840	47.743	731	327	9	75	799	7	688	635
MS.gene27084.t1	MTR_5g096430	48.718	702	319	8	78	775	5	669	618
MS.gene27084.t1	MTR_5g096460	48.718	702	319	8	78	775	5	669	618
MS.gene27084.t1	MTR_5g097320	46.164	743	345	15	77	804	89	791	597
MS.gene27084.t1	MTR_0009s0390	44.912	737	352	15	76	801	80	773	590

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MS.gene27084.t1	AT4G24190	83.184	779	126	3	1	778	1	775	1244
MS.gene27084.t1	AT4G24190	83.184	779	126	3	1	778	1	775	1243
MS.gene27084.t1	AT5G52640	49.041	730	330	8	75	799	2	694	648
MS.gene27084.t1	AT5G56000	47.765	716	333	7	75	786	2	680	627
MS.gene27084.t1	AT5G56010	47.943	705	326	7	75	775	2	669	617
MS.gene27084.t1	AT5G56030	47.943	705	326	7	75	775	2	669	616
MS.gene27084.t1	AT3G07770	47.525	707	321	13	77	773	94	760	610
MS.gene27084.t1	AT3G07770	47.525	707	321	13	77	773	94	760	610
MS.gene27084.t1	AT3G07770	47.525	707	321	13	77	773	94	760	610
MS.gene27084.t1	AT2G04030	45.200	750	338	17	75	799	74	775	602

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Find 170 sgRNAs with CRISPR-Local

Find 318 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTCGGTGCTGTTCCTCA+TGG	0.549531	1.1:+529746	MS.gene27084:CDS
AAATGACGATGGTGATGTCA+AGG	0.662375	1.1:+534451	MS.gene27084:CDS
AAATTTCAAGCGAATGCGGA+AGG	0.606036	1.1:+529683	MS.gene27084:CDS
AACTTCCCTATCTATATCTG+GGG	0.620396	1.1:+532089	MS.gene27084:CDS
AAGAGAGAGAAGAAGAAGAG+TGG	0.506230	1.1:-529443	None:intergenic
AAGAGTAGTTAAGAACCCTG+AGG	0.776155	1.1:+534108	MS.gene27084:CDS
AAGCAAAGAGGTTGATGTGG+AGG	0.610301	1.1:+532112	MS.gene27084:CDS
AAGCATACATGCGCGGCAAG+AGG	0.548258	1.1:+534038	MS.gene27084:CDS
AAGCTTACGGATCATATCAA+GGG	0.627367	1.1:-533023	None:intergenic
AAGTCCTGAGGTGCTTTAGG+TGG	0.640658	1.1:-532692	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACTTCAGGAAACATAGTT+AGG	-	chr1.1:531913-531932	None:intergenic	30.0%
AAGAATATAGGTGTTCTTGA+AGG	+	chr1.1:531645-531664	MS.gene27084:intron	30.0%
AAGCTAACATGGAAAACTTA+TGG	-	chr1.1:530758-530777	None:intergenic	30.0%
AATTTCTCTGCACATTTCAT+TGG	+	chr1.1:529544-529563	MS.gene27084:intron	30.0%
ACAAGCTGAAAACATATAAG+CGG	-	chr1.1:530733-530752	None:intergenic	30.0%
ACTATTTATGTGATACCCAA+CGG	+	chr1.1:532518-532537	MS.gene27084:intron	30.0%
ACTCAAAAAGCTCAAATACT+TGG	-	chr1.1:532807-532826	None:intergenic	30.0%
ATAAAGATATCCACTTCTCT+TGG	+	chr1.1:532546-532565	MS.gene27084:intron	30.0%
ATTGAAATGTGTGTGAATGA+AGG	+	chr1.1:529871-529890	MS.gene27084:intron	30.0%
ATTTCTCGTGATACATTCAA+TGG	-	chr1.1:532950-532969	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	529421	534484	529421	ID=MS.gene27084
chr1.1	mRNA	529421	534484	529421	ID=MS.gene27084.t1;Parent=MS.gene27084
chr1.1	exon	529421	529487	529421	ID=MS.gene27084.t1.exon1;Parent=MS.gene27084.t1
chr1.1	CDS	529421	529487	529421	ID=cds.MS.gene27084.t1;Parent=MS.gene27084.t1
chr1.1	exon	529678	529798	529678	ID=MS.gene27084.t1.exon2;Parent=MS.gene27084.t1
chr1.1	CDS	529678	529798	529678	ID=cds.MS.gene27084.t1;Parent=MS.gene27084.t1
chr1.1	exon	529893	530046	529893	ID=MS.gene27084.t1.exon3;Parent=MS.gene27084.t1
chr1.1	CDS	529893	530046	529893	ID=cds.MS.gene27084.t1;Parent=MS.gene27084.t1
chr1.1	exon	530452	530532	530452	ID=MS.gene27084.t1.exon4;Parent=MS.gene27084.t1
chr1.1	CDS	530452	530532	530452	ID=cds.MS.gene27084.t1;Parent=MS.gene27084.t1
chr1.1	exon	530884	530995	530884	ID=MS.gene27084.t1.exon5;Parent=MS.gene27084.t1
chr1.1	CDS	530884	530995	530884	ID=cds.MS.gene27084.t1;Parent=MS.gene27084.t1
chr1.1	exon	531086	531212	531086	ID=MS.gene27084.t1.exon6;Parent=MS.gene27084.t1
chr1.1	CDS	531086	531212	531086	ID=cds.MS.gene27084.t1;Parent=MS.gene27084.t1
chr1.1	exon	531316	531454	531316	ID=MS.gene27084.t1.exon7;Parent=MS.gene27084.t1
chr1.1	CDS	531316	531454	531316	ID=cds.MS.gene27084.t1;Parent=MS.gene27084.t1
chr1.1	exon	532059	532179	532059	ID=MS.gene27084.t1.exon8;Parent=MS.gene27084.t1
chr1.1	CDS	532059	532179	532059	ID=cds.MS.gene27084.t1;Parent=MS.gene27084.t1
chr1.1	exon	532307	532503	532307	ID=MS.gene27084.t1.exon9;Parent=MS.gene27084.t1
chr1.1	CDS	532307	532503	532307	ID=cds.MS.gene27084.t1;Parent=MS.gene27084.t1
chr1.1	exon	532611	532826	532611	ID=MS.gene27084.t1.exon10;Parent=MS.gene27084.t1
chr1.1	CDS	532611	532826	532611	ID=cds.MS.gene27084.t1;Parent=MS.gene27084.t1
chr1.1	exon	532923	533088	532923	ID=MS.gene27084.t1.exon11;Parent=MS.gene27084.t1
chr1.1	CDS	532923	533088	532923	ID=cds.MS.gene27084.t1;Parent=MS.gene27084.t1
chr1.1	exon	533232	533370	533232	ID=MS.gene27084.t1.exon12;Parent=MS.gene27084.t1
chr1.1	CDS	533232	533370	533232	ID=cds.MS.gene27084.t1;Parent=MS.gene27084.t1
chr1.1	exon	533467	533617	533467	ID=MS.gene27084.t1.exon13;Parent=MS.gene27084.t1
chr1.1	CDS	533467	533617	533467	ID=cds.MS.gene27084.t1;Parent=MS.gene27084.t1
chr1.1	exon	533725	534129	533725	ID=MS.gene27084.t1.exon14;Parent=MS.gene27084.t1
chr1.1	CDS	533725	534129	533725	ID=cds.MS.gene27084.t1;Parent=MS.gene27084.t1
chr1.1	exon	534224	534484	534224	ID=MS.gene27084.t1.exon15;Parent=MS.gene27084.t1
chr1.1	CDS	534224	534484	534224	ID=cds.MS.gene27084.t1;Parent=MS.gene27084.t1

Gene Sequence

>MS.gene27084

ATGAGGAAGTGGACGATTCCTTCCACTCTTCTTCTTCTCTCTCTTCTTCTTCTTCTCGCAGATCAAGGTTCGTTCATTCTTCATTCTTCAATTTCGTTTACGAATCGAAAGTTTGTTATGTATAATTTCTCTGCACATTTCATTGGTCGTTCATAGTTCAATTTCGTTTACGCATCGAAAATTTGTTATGAATAATTTTTACGCACATTTCATTTCAATGGTGGATTTCGTTTACTAAATGAAAATTTGTTTGTTAGGTCAAAAATTTCAAGCGAATGCGGAAGGTAATTCCGATGAAGTAGTAGATCCACCAAAGGTGGAAGAAAAACTCGGTGCTGTTCCTCATGGACTTTCAACCGATTCAGATGTTGTCAAGAGGTATCGATTTCTCTCTGATTTGAATTTCGATCTATGTTTCTATGTTTTTCTGTATTGATTTGTGATTTTGAGATTGAAATGTGTGTGAATGAAGGGAATCGGAGTCGATTTCGAAGAGATCCCTTCGCAGTAATGCGGAGAAGTTTGAGTTTCAAGCTGAAGTTTCAAGGCTTATGGATATTATCATCAATTCACTTTACAGTAATAAGGATATTTTCCTCAGGGAACTGATTTCAAATGCTTCTGATGTAAGCTCATTCTCTTTTACGTATATACATATATGCTCATTATGTACGTTTTGAGTTTTGAATCGGTGTGTGTTTAGTTCTGCGGTGACAAAAATTGAATAGGTAAAATTGATTATGGCATAAAGTGAGTTGAATGTAGAGTAATTTATGTTTGTATACATAGTCTAAAAATTAGTTTTAGAAACCAAAGCTTTACATTCTAACTTCAAGTTAGAATTGATTTTGACGGCAAAATCAATTATATTAGAGAGAAACCAACCATACATGTAAATCAATTCTACGCTTTTAGAATCAATTTTGGCTCCTCTCGAAGTGGAACCAAACATATACTTTGAAAATCCTTGCATTTATAATTTGTACATGAAATTGAACTTGTATTGATCACAAATAAGTTCTATATTGTAGGCACTGGACAAGATTAGGTTCCTATCCCTTACTGATAAAGACATTTTGGGTGAAGGTGACAACGCCAAGCTTGAAATCCAGGTTTGTATACTTTAATTATTATTATTATTATTCTATTTTCATTTAGTTAATTAGTTTGTCTCTGTAGGTTTTTTAATGATAAGGACCAAAGAATGATGGTGTTTTAATGCTGTTTTAATGGGTTTATTTTTGGACTGGCTTATATGAACTTATCTATTGGCTTAACTTGTAAAACTATTTGGGATAGCTTATTTAAACCGCTTATATGTTTTCAGCTTGTTTCCATAAGTTTTCCATGTTAGCTTATGAAAACCACTTATAGCTTATGTGAAAACAGTTTGACTTTATGCTATAAATTTAATTAATTTGTTTATCTAAAAAAGGTCTATATCTTGTTACTTACTGATGCAGATTAAGTTAGATAAGGAAAAGAAAATCCTATCTATTAGAGACAGAGGTATTGGTATGACAAAGGAGGATTTGGTAAAGAATTTGGGTACCATAGCAAAATCTGGAACTTCAGGTATGTATATAGTTTTGGGCTCTTAGCTGAAATGTTTGCCGTTTGTGATTTGGTGTGAAGGATTTTAATTGGAACTACGTTTGCTTGCAGCATTTGTTGAGAAAATGCAGACAAGTGGTGATCTCAATCTAATTGGGCAATTTGGAGTTGGTTTCTACTCTGTGTATCTTGTTGCTGACTATGTTGAAGTTATTAGCAAGCATAATGATGACAAACAGTGAGTGTTTTAGACTTGAAATTGCTTTTCTGATTGGTATGTTGTGGATGTTTTTGGAAATATTATGCCAGCCTATTGATTTTGGAAATGATCTGACTTTCAGGTATGTATGGGAATCTAAAGCTGATGGAGCATTTGCAATTTCAGAAGATACATGGAATGAACCACTAGGACGTGGAACAGAGATTAAAATACACCTCAAAGAAGAGGCCAGCGAGTACGTGGAAGAGTATAAGCTCAAAGTATGCCCCAAACTATCACTTTTATTATAACCAGTTGCTTGATTTTAAAATCACACGTGTTCTATTTCTATTTTAATAAACATAGTTCTTTATTTTTCACTGCATGGGTATCATTTAGTTTAACCTAGATGTCAACAATTTGCACTTTACATGTATAGCAAGTGATACTACCTACACTTTGTTCATGCTTAAGAATATAGGTGTTCTTGAAGGTTATAAATCAAATTTGTGGTGTCTTATAGCTTCTTTAACTCTCTGGCGTGCGTGCGTGCATAAGGCATGTGTTTTTGAAGTGATTGCACATGTTGTTGATTTTCATTGAGTAGATATACAATATCGTATACAATAATAAGTAGGGGGTAGTGTTTCTATGGAGTATGCATACCTTAGAAGGTAAACTCTTATGGGTTAAATTAAATTATCACAGGGTGATTGGTTTACGAAAACAGCACTCGCCTAACTATGTTTCCTGAAGTTTAAAAGATGGCAGAAAAAAAAAAAAAAAAAAGAGAAATCTGTTTTAATATATATTCTTTTACATTATCTATTCAATATACAATTGGTCTATTGCTAAATCTGGTTTAATAACTTCTGTTTTTCACAGGAATTGGTGAAGAGATACTCTGAATTTATTAACTTCCCTATCTATATCTGGGGAAGCAAAGAGGTTGATGTGGAGGTTCCCGCTGATGAAGACGACGAGTCTTCTGAGGAAGAGGATACAAGTATGTATAATTTAAGGATTCAGTTGGATCTTCATTTTTCAAGATTCTGTGTTTTGTTCGATATTAAAATTGATATATCTGTAGAAATTTAAGTTGTCTTTCGTACCATATTTTACTCTTCAATCAGCTGAAAGCCCCAAGGAGGAAAGTGAAGATGAAGATGCTGATAAAGATGAAGATGAAGAAAAAAAGCCTAAAACAAAGACAGTGAAGGAAACGACTTACGAGTGGGAACTCTTAAATGATGTGAAGGCTATATGGCTGAGGAATCCAAAGGAGGTTACAGAGGAAGAGTACACAAAATTCTACCACTCTCTTGCCAAGGTAATATTGCCATCACTATTTATGTGATACCCAACGGAACATATAAAGATATCCACTTCTCTTGGAGCTTGATGATACTTATAATGTTGGGGATTTGTGTTTTGCAGGATTTTAGCGATGACAAACCTTTATCATGGAGCCACTTTACTGCTGAAGGTGATGTCGAGTTCAAAGCAGTCCTTTATGTGCCACCTAAAGCACCTCAGGACTTGTACGAGAGTTATTACAATTCAAACAAATCCAACCTGAAGTTGTATGTTAGACGAGTGTTCATTTCAGATGAATTTGATGAATTGTTGCCCAAGTATTTGAGCTTTTTGAGTGTAAGTTTGATTTGCTGTGCTATTTTACATGTGTATATGTTATGAAGATCAGAGTAGTTTTTCTTAAACAAATGTTCTTTTTGGCATTGGGAACAGGGCCTTGTTGATTCTGACACTTTGCCATTGAATGTATCACGAGAAATGCTTCAACAACACAGCAGTCTGAAAACAATAAAAAAGAAACTTATTAGGAAAGCCCTTGATATGATCCGTAAGCTTGCCGAAGAGGACCCTGACGAGTCCACTGACAAAGAAAAGAAAGGTATGGTGTAATGACTGTTGGGTTTTATATGCAAGAATCCTAATTCTTTTATGGTTTCTCATCCCTTTTCAAATGTAAGCGAGCGGTTTCCTGAATTCCCTTCACTGACTTTTCGCTTCTACCAATTTTAACTTTTGATGCAGAAGAGACATCCTCTGATGTTGATGAGAAGAGAGGTCAATACACCAAGTTCTGGAATGAGTTTGGAAAATCTATTAAACTTGGTATCATTGAGGATGCCACCAACAGAAATCGTTTATCAAAACTGCTCAGATTTGAAACGTACTACTTCTTTCCTTGTTATTTAACTCTTATAGGAGTGTCATTTCCCCATTTCATCAGTTAACAATGTAGCTGATTCTTGGAACTTATTTACAGCACCAAGTCTGAGGGTAAATTGACATCTCTTGATCAGTACATATCTAGAATGAAAGCTGGACAGAAGGATATCTTTTACATAACTGGAACCAGCAAAGAACAACTGGAAAATTCACCATTCCTTGAGCGTCTGAAGAAGAAAAACTTTGAGGTGCAGCTTAGAAGTGTTTTGATTTTTATGCGAAGTCTTGTTTCCAGAAATTGTATTTATTTTGACATCTTCTAAGTGTATTTTTTTTTTTTCAATATATGCTACAGGTTATTTTCTTCACTGACCCAGTTGATGAATACTTGATGCAATACCTGATGGATTATGAAGATAAGAAGTTCCAAAATGTGTCCAAGGAGGGTCTGAAACTCGGGAAGGATTCTAAAGACAAGGAACTAAAGGAATCCTTCAAGGATCTAACTAAGTGGTGGAAAAATTCCCTTGCAAATGACAATGTCGATGATGTGAAGATATCCAACCGATTGGATAACACTCCTTGTGTAGTGGTGACATCAAAATTTGGTTGGAGTGCAAACATGGAGAGAATTATGCAGTCTCAAACTTTGTCAGATGCTAAAAAGCAAGCATACATGCGCGGCAAGAGGGTTCTTGAGATCAATCCTAGACACCCTATCATCAAGGAACTCCGGGAAAGAGTAGTTAAGAACCCTGAGGTACGTTCTAAATTTGAACTTTTGTATATTGCCCTTATTTTATGTCTTCAACATAGTTGGGCTTTTTGGTTTACTCAATTGGCTTACTTTTCAGGATGAGAGTGTGAAGCAAACGGCACAGCTGATGTACCAGACTGCACTTTTCGAAAGTGGTTTCTTGTTGAATGATCCCAAGGATTTTGCTTCTAGAATTTATGATTCAGTGAAGACTAGCTTAGACATCAGTCCTGATGCAACAGTTGAGGAGGAAGATGAGACTGAAGTTGAGGTTGAAAGTGAAACAAAAGAAGAAGCGCCTGCTTCAAACGAAGCTGATGAGGTAAATGACGATGGTGATGTCAAGGATGAGTTGTAG

Protein sequence

>MS.gene27084.t1

MRKWTIPSTLLLLSLLLLLADQGQKFQANAEGNSDEVVDPPKVEEKLGAVPHGLSTDSDVVKRESESISKRSLRSNAEKFEFQAEVSRLMDIIINSLYSNKDIFLRELISNASDALDKIRFLSLTDKDILGEGDNAKLEIQIKLDKEKKILSIRDRGIGMTKEDLVKNLGTIAKSGTSAFVEKMQTSGDLNLIGQFGVGFYSVYLVADYVEVISKHNDDKQYVWESKADGAFAISEDTWNEPLGRGTEIKIHLKEEASEYVEEYKLKELVKRYSEFINFPIYIWGSKEVDVEVPADEDDESSEEEDTTESPKEESEDEDADKDEDEEKKPKTKTVKETTYEWELLNDVKAIWLRNPKEVTEEEYTKFYHSLAKDFSDDKPLSWSHFTAEGDVEFKAVLYVPPKAPQDLYESYYNSNKSNLKLYVRRVFISDEFDELLPKYLSFLSGLVDSDTLPLNVSREMLQQHSSLKTIKKKLIRKALDMIRKLAEEDPDESTDKEKKEETSSDVDEKRGQYTKFWNEFGKSIKLGIIEDATNRNRLSKLLRFETTKSEGKLTSLDQYISRMKAGQKDIFYITGTSKEQLENSPFLERLKKKNFEVIFFTDPVDEYLMQYLMDYEDKKFQNVSKEGLKLGKDSKDKELKESFKDLTKWWKNSLANDNVDDVKISNRLDNTPCVVVTSKFGWSANMERIMQSQTLSDAKKQAYMRGKRVLEINPRHPIIKELRERVVKNPEDESVKQTAQLMYQTALFESGFLLNDPKDFASRIYDSVKTSLDISPDATVEEEDETEVEVESETKEEAPASNEADEVNDDGDVKDEL