Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30198.t1 | XP_013443297.1 | 82.3 | 831 | 51 | 2 | 5 | 835 | 1 | 735 | 2.10E-305 | 1058.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30198.t1 | Q96GN5 | 46.6 | 118 | 58 | 2 | 37 | 152 | 340 | 454 | 1.4e-23 | 113.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30198.t1 | A0A072TIS9 | 82.3 | 831 | 51 | 2 | 5 | 835 | 1 | 735 | 1.5e-305 | 1058.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene30198 | 0.864845 | 9.07E-65 | -1.69E-46 |
| MS.gene049159 | MS.gene30198 | 0.849292 | 3.55E-60 | -1.69E-46 |
| MS.gene049368 | MS.gene30198 | 0.835687 | 1.45E-56 | -1.69E-46 |
| MS.gene049435 | MS.gene30198 | 0.803268 | 3.78E-49 | -1.69E-46 |
| MS.gene049501 | MS.gene30198 | 0.830157 | 3.44E-55 | -1.69E-46 |
| MS.gene049821 | MS.gene30198 | 0.819103 | 1.38E-52 | -1.69E-46 |
| MS.gene049850 | MS.gene30198 | 0.832642 | 8.41E-56 | -1.69E-46 |
| MS.gene050442 | MS.gene30198 | 0.80086 | 1.18E-48 | -1.69E-46 |
| MS.gene050689 | MS.gene30198 | 0.801999 | 6.91E-49 | -1.69E-46 |
| MS.gene050901 | MS.gene30198 | 0.818675 | 1.73E-52 | -1.69E-46 |
| MS.gene051102 | MS.gene30198 | 0.814395 | 1.57E-51 | -1.69E-46 |
| MS.gene051198 | MS.gene30198 | 0.823832 | 1.12E-53 | -1.69E-46 |
| MS.gene051247 | MS.gene30198 | 0.852573 | 4.22E-61 | -1.69E-46 |
| MS.gene051490 | MS.gene30198 | 0.810365 | 1.19E-50 | -1.69E-46 |
| MS.gene05157 | MS.gene30198 | 0.830088 | 3.58E-55 | -1.69E-46 |
| MS.gene051664 | MS.gene30198 | 0.800978 | 1.12E-48 | -1.69E-46 |
| MS.gene051700 | MS.gene30198 | 0.804514 | 2.08E-49 | -1.69E-46 |
| MS.gene051719 | MS.gene30198 | 0.810649 | 1.04E-50 | -1.69E-46 |
| MS.gene051720 | MS.gene30198 | 0.82276 | 1.99E-53 | -1.69E-46 |
| MS.gene051744 | MS.gene30198 | 0.854826 | 9.51E-62 | -1.69E-46 |
| MS.gene051975 | MS.gene30198 | 0.812743 | 3.64E-51 | -1.69E-46 |
| MS.gene052012 | MS.gene30198 | 0.808803 | 2.59E-50 | -1.69E-46 |
| MS.gene052020 | MS.gene30198 | 0.839701 | 1.35E-57 | -1.69E-46 |
| MS.gene052463 | MS.gene30198 | 0.817168 | 3.79E-52 | -1.69E-46 |
| MS.gene052516 | MS.gene30198 | 0.826588 | 2.50E-54 | -1.69E-46 |
| MS.gene052609 | MS.gene30198 | 0.828134 | 1.06E-54 | -1.69E-46 |
| MS.gene052611 | MS.gene30198 | 0.800851 | 1.19E-48 | -1.69E-46 |
| MS.gene052773 | MS.gene30198 | 0.808595 | 2.87E-50 | -1.69E-46 |
| MS.gene052863 | MS.gene30198 | 0.815978 | 7.01E-52 | -1.69E-46 |
| MS.gene052896 | MS.gene30198 | 0.825867 | 3.71E-54 | -1.69E-46 |
| MS.gene053079 | MS.gene30198 | 0.819976 | 8.75E-53 | -1.69E-46 |
| MS.gene053144 | MS.gene30198 | 0.804526 | 2.07E-49 | -1.69E-46 |
| MS.gene05315 | MS.gene30198 | 0.824536 | 7.66E-54 | -1.69E-46 |
| MS.gene053226 | MS.gene30198 | 0.853066 | 3.06E-61 | -1.69E-46 |
| MS.gene053296 | MS.gene30198 | 0.813366 | 2.65E-51 | -1.69E-46 |
| MS.gene054236 | MS.gene30198 | 0.819809 | 9.56E-53 | -1.69E-46 |
| MS.gene054584 | MS.gene30198 | 0.804227 | 2.39E-49 | -1.69E-46 |
| MS.gene05458 | MS.gene30198 | 0.800527 | 1.38E-48 | -1.69E-46 |
| MS.gene05462 | MS.gene30198 | 0.815961 | 7.07E-52 | -1.69E-46 |
| MS.gene054651 | MS.gene30198 | 0.820157 | 7.95E-53 | -1.69E-46 |
| MS.gene05468 | MS.gene30198 | 0.804852 | 1.77E-49 | -1.69E-46 |
| MS.gene054775 | MS.gene30198 | 0.821472 | 3.97E-53 | -1.69E-46 |
| MS.gene055313 | MS.gene30198 | 0.809037 | 2.31E-50 | -1.69E-46 |
| MS.gene055465 | MS.gene30198 | 0.851218 | 1.02E-60 | -1.69E-46 |
| MS.gene055466 | MS.gene30198 | 0.864232 | 1.41E-64 | -1.69E-46 |
| MS.gene055468 | MS.gene30198 | 0.846832 | 1.70E-59 | -1.69E-46 |
| MS.gene055469 | MS.gene30198 | 0.845315 | 4.38E-59 | -1.69E-46 |
| MS.gene055513 | MS.gene30198 | 0.82602 | 3.41E-54 | -1.69E-46 |
| MS.gene055623 | MS.gene30198 | 0.81234 | 4.45E-51 | -1.69E-46 |
| MS.gene055778 | MS.gene30198 | 0.844574 | 6.95E-59 | -1.69E-46 |
| MS.gene055818 | MS.gene30198 | 0.827866 | 1.23E-54 | -1.69E-46 |
| MS.gene055864 | MS.gene30198 | 0.810529 | 1.10E-50 | -1.69E-46 |
| MS.gene056173 | MS.gene30198 | 0.81222 | 4.73E-51 | -1.69E-46 |
| MS.gene056174 | MS.gene30198 | 0.837283 | 5.70E-57 | -1.69E-46 |
| MS.gene056215 | MS.gene30198 | 0.858063 | 1.06E-62 | -1.69E-46 |
| MS.gene056308 | MS.gene30198 | 0.823645 | 1.24E-53 | -1.69E-46 |
| MS.gene056507 | MS.gene30198 | 0.81252 | 4.07E-51 | -1.69E-46 |
| MS.gene056595 | MS.gene30198 | 0.811708 | 6.12E-51 | -1.69E-46 |
| MS.gene05690 | MS.gene30198 | 0.816824 | 4.53E-52 | -1.69E-46 |
| MS.gene057034 | MS.gene30198 | 0.819467 | 1.14E-52 | -1.69E-46 |
| MS.gene057693 | MS.gene30198 | 0.800231 | 1.59E-48 | -1.69E-46 |
| MS.gene057869 | MS.gene30198 | 0.832951 | 7.05E-56 | -1.69E-46 |
| MS.gene057915 | MS.gene30198 | 0.867048 | 1.82E-65 | -1.69E-46 |
| MS.gene058010 | MS.gene30198 | 0.815749 | 7.88E-52 | -1.69E-46 |
| MS.gene058037 | MS.gene30198 | 0.808879 | 2.49E-50 | -1.69E-46 |
| MS.gene058292 | MS.gene30198 | 0.801092 | 1.06E-48 | -1.69E-46 |
| MS.gene05847 | MS.gene30198 | 0.812645 | 3.82E-51 | -1.69E-46 |
| MS.gene058625 | MS.gene30198 | 0.824852 | 6.45E-54 | -1.69E-46 |
| MS.gene059084 | MS.gene30198 | 0.825136 | 5.53E-54 | -1.69E-46 |
| MS.gene059170 | MS.gene30198 | 0.80104 | 1.09E-48 | -1.69E-46 |
| MS.gene059331 | MS.gene30198 | 0.810493 | 1.12E-50 | -1.69E-46 |
| MS.gene059439 | MS.gene30198 | 0.802047 | 6.75E-49 | -1.69E-46 |
| MS.gene059570 | MS.gene30198 | 0.821032 | 5.01E-53 | -1.69E-46 |
| MS.gene059625 | MS.gene30198 | 0.83604 | 1.18E-56 | -1.69E-46 |
| MS.gene059725 | MS.gene30198 | 0.814394 | 1.58E-51 | -1.69E-46 |
| MS.gene059842 | MS.gene30198 | 0.824583 | 7.46E-54 | -1.69E-46 |
| MS.gene060167 | MS.gene30198 | 0.812231 | 4.70E-51 | -1.69E-46 |
| MS.gene060310 | MS.gene30198 | 0.804716 | 1.89E-49 | -1.69E-46 |
| MS.gene060429 | MS.gene30198 | 0.813304 | 2.74E-51 | -1.69E-46 |
| MS.gene06052 | MS.gene30198 | 0.809394 | 1.93E-50 | -1.69E-46 |
| MS.gene060637 | MS.gene30198 | 0.831627 | 1.50E-55 | -1.69E-46 |
| MS.gene060803 | MS.gene30198 | 0.813746 | 2.19E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene30201 | MS.gene30198 | PPI |
| MS.gene074288 | MS.gene30198 | PPI |
| MS.gene074289 | MS.gene30198 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30198.t1 | MTR_0024s0330 | 87.960 | 598 | 55 | 2 | 238 | 835 | 155 | 735 | 0.0 | 996 |
| MS.gene30198.t1 | MTR_0024s0330 | 81.564 | 358 | 36 | 3 | 5 | 345 | 1 | 345 | 1.96e-177 | 529 |
| MS.gene30198.t1 | MTR_6g017265 | 72.436 | 468 | 94 | 2 | 398 | 834 | 171 | 634 | 0.0 | 657 |
| MS.gene30198.t1 | MTR_6g017265 | 69.136 | 243 | 54 | 4 | 5 | 238 | 1 | 231 | 3.01e-95 | 311 |
| MS.gene30198.t1 | MTR_6g017265 | 74.000 | 50 | 13 | 0 | 296 | 345 | 165 | 214 | 1.91e-11 | 68.2 |
| MS.gene30198.t1 | MTR_5g018400 | 42.647 | 136 | 73 | 2 | 33 | 166 | 148 | 280 | 3.68e-26 | 112 |
| MS.gene30198.t1 | MTR_5g018470 | 41.304 | 138 | 71 | 3 | 33 | 166 | 150 | 281 | 9.29e-25 | 108 |
| MS.gene30198.t1 | MTR_4g087240 | 36.066 | 122 | 67 | 3 | 38 | 154 | 171 | 286 | 8.52e-17 | 82.8 |
| MS.gene30198.t1 | MTR_6g070950 | 39.640 | 111 | 44 | 3 | 41 | 128 | 167 | 277 | 7.56e-14 | 73.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30198.t1 | AT5G38690 | 39.910 | 446 | 248 | 9 | 387 | 825 | 125 | 557 | 1.91e-80 | 270 |
| MS.gene30198.t1 | AT5G38690 | 62.385 | 109 | 41 | 0 | 39 | 147 | 21 | 129 | 8.97e-41 | 159 |
| MS.gene30198.t1 | AT5G38690 | 39.910 | 446 | 248 | 9 | 387 | 825 | 125 | 557 | 1.91e-80 | 270 |
| MS.gene30198.t1 | AT5G38690 | 62.385 | 109 | 41 | 0 | 39 | 147 | 21 | 129 | 8.97e-41 | 159 |
| MS.gene30198.t1 | AT1G67780 | 38.272 | 324 | 184 | 6 | 508 | 825 | 185 | 498 | 4.46e-54 | 196 |
| MS.gene30198.t1 | AT1G67780 | 69.298 | 114 | 35 | 0 | 28 | 141 | 13 | 126 | 1.02e-48 | 181 |
| MS.gene30198.t1 | AT1G67780 | 38.272 | 324 | 184 | 6 | 508 | 825 | 185 | 498 | 4.46e-54 | 196 |
| MS.gene30198.t1 | AT1G67780 | 69.298 | 114 | 35 | 0 | 28 | 141 | 13 | 126 | 1.02e-48 | 181 |
| MS.gene30198.t1 | AT1G67270 | 38.782 | 312 | 180 | 4 | 508 | 817 | 181 | 483 | 1.08e-52 | 191 |
| MS.gene30198.t1 | AT1G67270 | 64.912 | 114 | 40 | 0 | 28 | 141 | 5 | 118 | 7.23e-44 | 166 |
| MS.gene30198.t1 | AT2G23530 | 46.729 | 107 | 48 | 2 | 38 | 141 | 153 | 253 | 6.71e-24 | 107 |
| MS.gene30198.t1 | AT4G37110 | 39.130 | 138 | 67 | 2 | 11 | 148 | 64 | 184 | 2.27e-22 | 99.4 |
| MS.gene30198.t1 | AT4G37110 | 39.130 | 138 | 67 | 2 | 11 | 148 | 64 | 184 | 2.27e-22 | 99.4 |
| MS.gene30198.t1 | AT4G37110 | 41.085 | 129 | 59 | 2 | 13 | 141 | 161 | 272 | 7.97e-22 | 99.8 |
| MS.gene30198.t1 | AT4G37110 | 41.085 | 129 | 59 | 2 | 13 | 141 | 148 | 259 | 8.06e-22 | 99.4 |
Find 187 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGCTTGTGGTAGATCTATT+TGG | 0.184927 | 6.3:-13284104 | MS.gene30198:CDS |
| ATAGATTTAAAGGTTGAAAA+TGG | 0.190065 | 6.3:-13283765 | MS.gene30198:CDS |
| AAATGCGGAGTTCGTGTTAT+TGG | 0.198381 | 6.3:-13286449 | MS.gene30198:CDS |
| CCTACTGAATTCTGCTTTGT+AGG | 0.203177 | 6.3:+13283142 | None:intergenic |
| TCCAGAGTAGCTTCCTTTAC+AGG | 0.213413 | 6.3:+13284308 | None:intergenic |
| ATCGATTATGGCTACTAAAA+TGG | 0.214036 | 6.3:-13286546 | None:intergenic |
| AAACTCATGGTTGAAAGTTT+TGG | 0.234305 | 6.3:-13280897 | MS.gene30198:CDS |
| CCTACTGAATTCTGCTTCAT+AGG | 0.236786 | 6.3:+13283492 | None:intergenic |
| ACTTGTGGCAGAACTTAATT+TGG | 0.257589 | 6.3:+13284793 | None:intergenic |
| AGGTGGAATTATAGGTTTAA+AGG | 0.257983 | 6.3:-13283433 | MS.gene30198:CDS |
| CCTACTGAATTCTGCTTCAT+AGG | 0.263950 | 6.3:+13283453 | None:intergenic |
| AAAGAAGGCTTCTGAAAGTT+TGG | 0.270786 | 6.3:-13284354 | MS.gene30198:CDS |
| TGCAAATGCCTCTGCACTTT+GGG | 0.282113 | 6.3:+13284574 | None:intergenic |
| AGGTGAAAACATAGGTTTAA+AGG | 0.283661 | 6.3:-13282790 | MS.gene30198:CDS |
| AGGTGAAAACATAGGTTTAA+AGG | 0.283661 | 6.3:-13283122 | MS.gene30198:CDS |
| TGGCAAGGCTTCCCAGTTCT+TGG | 0.285176 | 6.3:+13284813 | None:intergenic |
| CCGTCGGATTGGCTTAATAA+AGG | 0.290448 | 6.3:-13280833 | MS.gene30198:CDS |
| AGGTGGAAACATAGATTTAA+AGG | 0.290482 | 6.3:-13283775 | MS.gene30198:CDS |
| AGGTGGAAACAAAGGTTTAA+AGG | 0.298135 | 6.3:-13284057 | MS.gene30198:CDS |
| GAGCTTGTAGTGGATCTATC+TGG | 0.299492 | 6.3:-13283822 | MS.gene30198:CDS |
| AGCTTGTGGTAGATCTATTT+GGG | 0.300184 | 6.3:-13284103 | MS.gene30198:CDS |
| GAGCTTGTAGTGGATCTATC+TGG | 0.303761 | 6.3:-13282837 | MS.gene30198:CDS |
| TCAAAGTCAACAGTTTGATT+TGG | 0.305707 | 6.3:+13281100 | None:intergenic |
| ATAACAATGGTAAGGCATTC+TGG | 0.307965 | 6.3:-13279623 | MS.gene30198:CDS |
| AATTCAGTAGGTGGAATTAT+AGG | 0.311137 | 6.3:-13283441 | MS.gene30198:CDS |
| ACCTAGTGAATTCAGCTTCC+CGG | 0.316117 | 6.3:+13284076 | None:intergenic |
| CCCGTTTCAGAATTTGCTTT+TGG | 0.321715 | 6.3:+13282048 | None:intergenic |
| CGAATCTATGATTCTGCTAA+TGG | 0.326535 | 6.3:-13286422 | MS.gene30198:CDS |
| AAAGCTGACTTCAACCTTCT+AGG | 0.326724 | 6.3:-13279350 | None:intergenic |
| TTAAATTAAATATCCCTAGA+AGG | 0.340416 | 6.3:+13279336 | None:intergenic |
| GCCACAAGTGCCTCTTGAAC+AGG | 0.347791 | 6.3:-13284779 | MS.gene30198:intron |
| TTAGAATTCTGCAGATTGTT+TGG | 0.349053 | 6.3:-13281737 | MS.gene30198:CDS |
| AAAGAAAGCAGCAAAGTCTA+AGG | 0.350593 | 6.3:-13280589 | MS.gene30198:CDS |
| AGTGAAGCGGGAAAACTTAA+AGG | 0.370001 | 6.3:-13282166 | MS.gene30198:CDS |
| AAAATTAAAGGAAGCTACTC+TGG | 0.377113 | 6.3:-13283376 | MS.gene30198:CDS |
| CATAAGTGGATATTATGATT+TGG | 0.388807 | 6.3:-13280810 | MS.gene30198:CDS |
| GTAGATCTATTTGGGGAAAC+CGG | 0.389454 | 6.3:-13284095 | MS.gene30198:CDS |
| GCAAAAGCTGTTGTATCAAA+TGG | 0.390668 | 6.3:-13280410 | MS.gene30198:CDS |
| ACATTTCCTCTAGGAGAGAT+TGG | 0.391866 | 6.3:-13279378 | MS.gene30198:CDS |
| AATGCATTCCCAACATCTTC+TGG | 0.397485 | 6.3:+13281767 | None:intergenic |
| CCAAAAGCAAATTCTGAAAC+GGG | 0.403615 | 6.3:-13282048 | MS.gene30198:CDS |
| GAGAATCGAGCCGGAGTACT+TGG | 0.407702 | 6.3:-13279646 | MS.gene30198:CDS |
| AAAGTTAAAGGAAGCTACTC+TGG | 0.410652 | 6.3:-13283065 | MS.gene30198:CDS |
| AGGATTACCTTGTAACATAT+AGG | 0.410945 | 6.3:+13279572 | None:intergenic |
| GTGGATCTATCTGGGGAACC+TGG | 0.413007 | 6.3:-13282828 | MS.gene30198:CDS |
| GCTTGTAAGAATCCAAGAAC+TGG | 0.414838 | 6.3:-13284825 | MS.gene30198:CDS |
| AAGTCTTAAGCAAAAGTTGC+AGG | 0.414891 | 6.3:-13280441 | MS.gene30198:CDS |
| CCTACTGAATTCTGCTTCCC+AGG | 0.418681 | 6.3:+13282810 | None:intergenic |
| TAGATTTAAAGGTTGAAAAT+GGG | 0.419153 | 6.3:-13283764 | MS.gene30198:CDS |
| GAAACTACATTAGAAAGTTT+AGG | 0.426474 | 6.3:+13282087 | None:intergenic |
| GTTCATTCAGCCAAGGCATC+CGG | 0.432115 | 6.3:-13284404 | MS.gene30198:CDS |
| TTGCAAATGCCTCTGCACTT+TGG | 0.438657 | 6.3:+13284573 | None:intergenic |
| ATTCTGCTTTGTAGGTTCCC+TGG | 0.442441 | 6.3:+13283150 | None:intergenic |
| AGGTAGAACTATTGAATGAT+TGG | 0.447488 | 6.3:-13284602 | MS.gene30198:CDS |
| GCCTGTAAAGGAAGCTACTC+TGG | 0.449925 | 6.3:-13284309 | MS.gene30198:CDS |
| GAGCTTGTAGTGGATCTATC+CGG | 0.450636 | 6.3:-13283519 | MS.gene30198:CDS |
| AGTTTGCACCAGAAGATGTT+GGG | 0.451171 | 6.3:-13281775 | MS.gene30198:CDS |
| TATACTACAGGAGGTTGTCT+TGG | 0.454756 | 6.3:-13282268 | MS.gene30198:CDS |
| AGGTGGAGTTGGTGCTTCAA+AGG | 0.454999 | 6.3:-13282211 | MS.gene30198:CDS |
| AGCTTGTAGTGGATCTATCC+AGG | 0.459153 | 6.3:-13283168 | MS.gene30198:CDS |
| GAAAACATAGGTTTAAAGGT+TGG | 0.464712 | 6.3:-13282786 | MS.gene30198:CDS |
| TAGAAGGTTGAAGTCAGCTT+TGG | 0.464863 | 6.3:+13279352 | None:intergenic |
| TTTATTTGTGATGAAGCCTT+GGG | 0.467960 | 6.3:-13280758 | MS.gene30198:CDS |
| TAAAAGAATTTCCGTCGGAT+TGG | 0.472570 | 6.3:-13280844 | MS.gene30198:CDS |
| ACCAAAAGCAAATTCTGAAA+CGG | 0.474696 | 6.3:-13282049 | MS.gene30198:CDS |
| TTTACCATTTGTCTGGGACT+TGG | 0.476747 | 6.3:+13281874 | None:intergenic |
| GGAGTTGGTGCTTCAAAGGT+TGG | 0.480973 | 6.3:-13282207 | MS.gene30198:CDS |
| GTCTAAGGTTTCTGAAGCTA+AGG | 0.481278 | 6.3:-13280574 | MS.gene30198:CDS |
| CGAATGGAAAGAAATTGAAT+CGG | 0.484997 | 6.3:-13286478 | MS.gene30198:CDS |
| CCTACTGAATTCTGCTTCGC+AGG | 0.487558 | 6.3:+13283795 | None:intergenic |
| TTGGGTCTATCAAGTGAACC+TGG | 0.487801 | 6.3:-13282249 | MS.gene30198:CDS |
| TGCTCAGGAACTGTTGTGAT+AGG | 0.488259 | 6.3:-13281917 | MS.gene30198:intron |
| TAATGGCAAAACTTGTCATC+AGG | 0.489532 | 6.3:-13286405 | MS.gene30198:intron |
| AAAGGAAGATACTCTGGAAA+AGG | 0.490343 | 6.3:-13283712 | MS.gene30198:intron |
| AAAGTTAAAGGAAGATACTC+TGG | 0.491926 | 6.3:-13283718 | MS.gene30198:CDS |
| ATACTACAGGAGGTTGTCTT+GGG | 0.492173 | 6.3:-13282267 | MS.gene30198:CDS |
| TGGCTTAATAAAGGCATAAG+TGG | 0.493521 | 6.3:-13280824 | MS.gene30198:CDS |
| AAGCTGACTTCAACCTTCTA+GGG | 0.496061 | 6.3:-13279349 | None:intergenic |
| AGCTTGTAGTGGATCTATCT+GGG | 0.497046 | 6.3:-13282836 | MS.gene30198:CDS |
| AGCTTGTAGTGGATCTATCT+GGG | 0.497046 | 6.3:-13283821 | MS.gene30198:CDS |
| CCTGGGAAAGAAAACTCCTT+AGG | 0.498484 | 6.3:-13282231 | MS.gene30198:CDS |
| AGAGTTGAAACCGGATGCCT+TGG | 0.498717 | 6.3:+13284394 | None:intergenic |
| ACCGAGTTGGTTGAGGCAAA+AGG | 0.498720 | 6.3:-13280317 | MS.gene30198:CDS |
| AAAGGAAGCTACTCTGGAAA+AGG | 0.499075 | 6.3:-13284303 | MS.gene30198:intron |
| AGCTTGTAGTGGATCTATCC+GGG | 0.503801 | 6.3:-13283518 | MS.gene30198:CDS |
| TGCTGACACAGAGTTGAAAC+CGG | 0.505301 | 6.3:+13284385 | None:intergenic |
| AATTCAGTAGGTGAAAATAT+AGG | 0.505670 | 6.3:-13283480 | MS.gene30198:CDS |
| ATATGGTGAGAAGGCAGAAG+AGG | 0.508169 | 6.3:-13284622 | MS.gene30198:CDS |
| GGTTTCTGAAGCTAAGGAAA+AGG | 0.511109 | 6.3:-13280568 | MS.gene30198:intron |
| TTGATATATTCAGGAAGCTA+AGG | 0.515389 | 6.3:-13280650 | MS.gene30198:intron |
| CTTTCTAATGTAGTTTCAGA+GGG | 0.522769 | 6.3:-13282081 | MS.gene30198:CDS |
| CACAAGAACTGAATGTGAAA+GGG | 0.523171 | 6.3:-13286508 | MS.gene30198:CDS |
| GAAAACTCCTTAGGTGGAGT+TGG | 0.525493 | 6.3:-13282222 | MS.gene30198:CDS |
| GATTTGGAACTCAACCACTA+AGG | 0.525815 | 6.3:+13281116 | None:intergenic |
| GGTGCCAAGTCCCAGACAAA+TGG | 0.526799 | 6.3:-13281878 | MS.gene30198:CDS |
| TTCTCACTGCATCACAATGT+TGG | 0.527421 | 6.3:+13279664 | None:intergenic |
| TAGATCTATTTGGGGAAACC+GGG | 0.529177 | 6.3:-13284094 | MS.gene30198:CDS |
| GAGTTTGCACCAGAAGATGT+TGG | 0.533090 | 6.3:-13281776 | MS.gene30198:CDS |
| GTCAGCAATGCTTAGAAAGA+AGG | 0.535931 | 6.3:-13284369 | MS.gene30198:CDS |
| AGCTCATACCGAGTTGGTTG+AGG | 0.535974 | 6.3:-13280324 | MS.gene30198:CDS |
| TGGCAGAACTTAATTTGGCA+AGG | 0.536819 | 6.3:+13284798 | None:intergenic |
| TTAATAAGTACATTTCCTCT+AGG | 0.538395 | 6.3:-13279387 | MS.gene30198:CDS |
| TCACAAGAACTGAATGTGAA+AGG | 0.539022 | 6.3:-13286509 | MS.gene30198:CDS |
| TGGAGCTGAGCAGAAGGATG+AGG | 0.539326 | 6.3:-13279410 | MS.gene30198:CDS |
| AACCAAGAAAGTGACGGAAA+AGG | 0.541227 | 6.3:-13284021 | MS.gene30198:intron |
| AAAGGAAGCTACTCTGGGAA+AGG | 0.544797 | 6.3:-13283059 | MS.gene30198:intron |
| AAAGGAAGCTACTCTGGGAA+AGG | 0.544797 | 6.3:-13283370 | MS.gene30198:intron |
| CTTCAACACCATCTTCATCA+TGG | 0.545569 | 6.3:+13279455 | None:intergenic |
| ATCATCAACACTGTTGCCAT+TGG | 0.546992 | 6.3:+13282137 | None:intergenic |
| TAGGCTCTTGATGTCAAGAA+AGG | 0.547393 | 6.3:-13281201 | MS.gene30198:CDS |
| ATAAACCAAACCTGTTCAAG+AGG | 0.552710 | 6.3:+13284769 | None:intergenic |
| ACTTTCTAATGTAGTTTCAG+AGG | 0.558234 | 6.3:-13282082 | MS.gene30198:CDS |
| TGGATCTATCTGGGGAACCT+GGG | 0.558717 | 6.3:-13282827 | MS.gene30198:CDS |
| ACCTGTTCAAGAGGCACTTG+TGG | 0.559250 | 6.3:+13284778 | None:intergenic |
| TCAGATTCAGGAGCTTGTAG+TGG | 0.560478 | 6.3:-13283179 | MS.gene30198:intron |
| ACGTGGACAAAACACCTTAG+TGG | 0.560975 | 6.3:-13281130 | MS.gene30198:CDS |
| CCTGTAGTATATACTTCCTC+CGG | 0.560982 | 6.3:+13282280 | None:intergenic |
| TATATTTCAGGAGCTTGTAG+TGG | 0.563462 | 6.3:-13282847 | MS.gene30198:intron |
| TATATTTCAGGAGCTTGTAG+TGG | 0.563462 | 6.3:-13283529 | MS.gene30198:intron |
| AAAGGAAACTACTCTAGGAA+AGG | 0.563977 | 6.3:-13282727 | MS.gene30198:intron |
| ATTCTGCTTCATAGGTTCCC+CGG | 0.564942 | 6.3:+13283500 | None:intergenic |
| GAAAACCAAGAAAGTGAAGC+GGG | 0.567532 | 6.3:-13282178 | MS.gene30198:CDS |
| TGAAGCACCAACTCCACCTA+AGG | 0.568130 | 6.3:+13282215 | None:intergenic |
| CCGGAGGAAGTATATACTAC+AGG | 0.570771 | 6.3:-13282280 | MS.gene30198:CDS |
| GTACTTGGATAACAATGGTA+AGG | 0.570868 | 6.3:-13279631 | MS.gene30198:CDS |
| GAATATGCCTATATGTTACA+AGG | 0.571950 | 6.3:-13279579 | MS.gene30198:intron |
| TGTTTATGGAGCTGAGCAGA+AGG | 0.575038 | 6.3:-13279416 | MS.gene30198:CDS |
| CTTGTAAGAATCCAAGAACT+GGG | 0.576666 | 6.3:-13284824 | MS.gene30198:CDS |
| AGAAAACCAAGAAAGTGAAG+CGG | 0.576898 | 6.3:-13282179 | MS.gene30198:CDS |
| AAGTTAAAGGAAACTACTCT+AGG | 0.578905 | 6.3:-13282732 | MS.gene30198:CDS |
| AAATTAAAGGAAGCTACTCT+GGG | 0.582970 | 6.3:-13283375 | MS.gene30198:CDS |
| AAGTTAAAGGAAGCTACTCT+GGG | 0.583380 | 6.3:-13283064 | MS.gene30198:CDS |
| AAAAGAGGTGAACAACCTAC+TGG | 0.584751 | 6.3:-13284434 | MS.gene30198:CDS |
| CATCACAAATAAAATTCAGA+AGG | 0.585281 | 6.3:+13280768 | None:intergenic |
| TGAATTTAGATATGGTGAGA+AGG | 0.586700 | 6.3:-13284631 | MS.gene30198:intron |
| AAATACTTACATCGTGCCCA+AGG | 0.591448 | 6.3:+13280742 | None:intergenic |
| CCTATGAAGCAGAATTCAGT+AGG | 0.595536 | 6.3:-13283453 | MS.gene30198:CDS |
| AGCTAAAGCTCATACCGAGT+TGG | 0.595550 | 6.3:-13280330 | MS.gene30198:CDS |
| TTATATAATACAGGAGCTTG+TGG | 0.595987 | 6.3:-13284117 | MS.gene30198:intron |
| AATTCAGTAGGTGAAAACAT+AGG | 0.596996 | 6.3:-13282798 | MS.gene30198:CDS |
| AATTCAGTAGGTGAAAACAT+AGG | 0.596996 | 6.3:-13283130 | MS.gene30198:CDS |
| ATTCTGCAGATTGTTTGGAA+AGG | 0.597032 | 6.3:-13281732 | MS.gene30198:intron |
| GGGAAAGAAAACTCCTTAGG+TGG | 0.598078 | 6.3:-13282228 | MS.gene30198:CDS |
| CCTACAAAGCAGAATTCAGT+AGG | 0.601636 | 6.3:-13283142 | MS.gene30198:CDS |
| TATAATACAGGAGCTTGTAG+TGG | 0.603396 | 6.3:-13283832 | MS.gene30198:intron |
| ATAGGAGATGGTGACAATGC+TGG | 0.603532 | 6.3:-13281899 | MS.gene30198:CDS |
| CCTATGAAGCAGAATTCAGT+AGG | 0.603826 | 6.3:-13283492 | MS.gene30198:CDS |
| TGATGCAGTGAGAATCGAGC+CGG | 0.604885 | 6.3:-13279655 | MS.gene30198:CDS |
| CATCGTGTTCTGAAATTGAG+AGG | 0.604942 | 6.3:+13280381 | None:intergenic |
| AACTTAAAGGAAATATCCAA+TGG | 0.606792 | 6.3:-13282153 | MS.gene30198:CDS |
| TCAGCCATAACTGCTAGCAA+TGG | 0.613966 | 6.3:-13280923 | MS.gene30198:CDS |
| CCTGGGAAGCAGAATTCAGT+AGG | 0.614086 | 6.3:-13282810 | MS.gene30198:CDS |
| AATTCACTAGGTGGAAACAA+AGG | 0.618686 | 6.3:-13284065 | MS.gene30198:CDS |
| CCATTGTTATCCAAGTACTC+CGG | 0.619911 | 6.3:+13279636 | None:intergenic |
| CCTGCGAAGCAGAATTCAGT+AGG | 0.620601 | 6.3:-13283795 | MS.gene30198:CDS |
| GTGAAAGGGAGTGATGCGAA+TGG | 0.625227 | 6.3:-13286494 | MS.gene30198:CDS |
| TTTGGAATCGAACAATGCTG+TGG | 0.625301 | 6.3:-13284336 | MS.gene30198:CDS |
| ATGAAGATCCATGATGAAGA+TGG | 0.630584 | 6.3:-13279463 | MS.gene30198:CDS |
| TGAGAAAACCAAGAAAGTGA+CGG | 0.630755 | 6.3:-13284027 | MS.gene30198:CDS |
| AGAAAACCAAGACAATGAAG+CGG | 0.634544 | 6.3:-13283090 | MS.gene30198:CDS |
| AGAAAACCAAGACAATGAAG+CGG | 0.634544 | 6.3:-13283401 | MS.gene30198:CDS |
| GCTTGTAGTGGATCTATCCA+GGG | 0.637847 | 6.3:-13283167 | MS.gene30198:CDS |
| ACCGGGAAGCTGAATTCACT+AGG | 0.643409 | 6.3:-13284077 | MS.gene30198:CDS |
| TGGGTCTATCAAGTGAACCT+GGG | 0.644898 | 6.3:-13282248 | MS.gene30198:CDS |
| GGAACTGTTGTGATAGGAGA+TGG | 0.646822 | 6.3:-13281911 | MS.gene30198:CDS |
| ATGAAGCAGAATTCAGTAGG+TGG | 0.650442 | 6.3:-13283450 | MS.gene30198:CDS |
| CCGGAGTACTTGGATAACAA+TGG | 0.653215 | 6.3:-13279636 | MS.gene30198:CDS |
| TGTACTAAAAGAATTTCCGT+CGG | 0.654987 | 6.3:-13280849 | MS.gene30198:CDS |
| TATACTAGTTCATTCAGCCA+AGG | 0.657723 | 6.3:-13284411 | MS.gene30198:CDS |
| GCGAAGCAGAATTCAGTAGG+TGG | 0.661073 | 6.3:-13283792 | MS.gene30198:CDS |
| TTCTTATAAATAAGGACCGG+AGG | 0.662465 | 6.3:-13282296 | MS.gene30198:intron |
| GAATGAACTAGTATACCAGT+AGG | 0.672480 | 6.3:+13284419 | None:intergenic |
| TGGTAACCAATCTCTCCTAG+AGG | 0.673606 | 6.3:+13279372 | None:intergenic |
| CCTTTATTAAGCCAATCCGA+CGG | 0.674604 | 6.3:+13280833 | None:intergenic |
| GGAAAACCAAGACAATGAAG+CGG | 0.677635 | 6.3:-13283743 | MS.gene30198:CDS |
| TTGAATCGGAACAATAAATG+CGG | 0.678075 | 6.3:-13286464 | MS.gene30198:CDS |
| CAAAAGCAAATTCTGAAACG+GGG | 0.680931 | 6.3:-13282047 | MS.gene30198:CDS |
| GCTTGTAGTGGATCTATCTG+GGG | 0.681442 | 6.3:-13282835 | MS.gene30198:CDS |
| GCTTGTAGTGGATCTATCTG+GGG | 0.681442 | 6.3:-13283820 | MS.gene30198:CDS |
| CGTAAGCAAAATTTGCGACG+TGG | 0.687278 | 6.3:-13281147 | MS.gene30198:CDS |
| GAGGAAGTATATACTACAGG+AGG | 0.692587 | 6.3:-13282277 | MS.gene30198:CDS |
| GCTTGTGGTAGATCTATTTG+GGG | 0.695308 | 6.3:-13284102 | MS.gene30198:CDS |
| GCTTGTAGTGGATCTATCCG+GGG | 0.698456 | 6.3:-13283517 | MS.gene30198:CDS |
| TGGATGTGCCCAAAGTGCAG+AGG | 0.714972 | 6.3:-13284582 | MS.gene30198:CDS |
| TTGTCATCTCAGTGCCAAGG+GGG | 0.718267 | 6.3:+13281813 | None:intergenic |
| GGGAAGCTGAATTCACTAGG+TGG | 0.722903 | 6.3:-13284074 | MS.gene30198:CDS |
| TTTGTCATCTCAGTGCCAAG+GGG | 0.736357 | 6.3:+13281812 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 13279351 | 13286561 | 13279351 | ID=MS.gene30198 |
| chr6.3 | mRNA | 13279351 | 13286561 | 13279351 | ID=MS.gene30198.t1;Parent=MS.gene30198 |
| chr6.3 | exon | 13286406 | 13286561 | 13286406 | ID=MS.gene30198.t1.exon1;Parent=MS.gene30198.t1 |
| chr6.3 | CDS | 13286406 | 13286561 | 13286406 | ID=cds.MS.gene30198.t1;Parent=MS.gene30198.t1 |
| chr6.3 | exon | 13284780 | 13284877 | 13284780 | ID=MS.gene30198.t1.exon2;Parent=MS.gene30198.t1 |
| chr6.3 | CDS | 13284780 | 13284877 | 13284780 | ID=cds.MS.gene30198.t1;Parent=MS.gene30198.t1 |
| chr6.3 | exon | 13284558 | 13284644 | 13284558 | ID=MS.gene30198.t1.exon3;Parent=MS.gene30198.t1 |
| chr6.3 | CDS | 13284558 | 13284644 | 13284558 | ID=cds.MS.gene30198.t1;Parent=MS.gene30198.t1 |
| chr6.3 | exon | 13284304 | 13284460 | 13284304 | ID=MS.gene30198.t1.exon4;Parent=MS.gene30198.t1 |
| chr6.3 | CDS | 13284304 | 13284460 | 13284304 | ID=cds.MS.gene30198.t1;Parent=MS.gene30198.t1 |
| chr6.3 | exon | 13284022 | 13284126 | 13284022 | ID=MS.gene30198.t1.exon5;Parent=MS.gene30198.t1 |
| chr6.3 | CDS | 13284022 | 13284126 | 13284022 | ID=cds.MS.gene30198.t1;Parent=MS.gene30198.t1 |
| chr6.3 | exon | 13283713 | 13283844 | 13283713 | ID=MS.gene30198.t1.exon6;Parent=MS.gene30198.t1 |
| chr6.3 | CDS | 13283713 | 13283844 | 13283713 | ID=cds.MS.gene30198.t1;Parent=MS.gene30198.t1 |
| chr6.3 | exon | 13283371 | 13283541 | 13283371 | ID=MS.gene30198.t1.exon7;Parent=MS.gene30198.t1 |
| chr6.3 | CDS | 13283371 | 13283541 | 13283371 | ID=cds.MS.gene30198.t1;Parent=MS.gene30198.t1 |
| chr6.3 | exon | 13283060 | 13283191 | 13283060 | ID=MS.gene30198.t1.exon8;Parent=MS.gene30198.t1 |
| chr6.3 | CDS | 13283060 | 13283191 | 13283060 | ID=cds.MS.gene30198.t1;Parent=MS.gene30198.t1 |
| chr6.3 | exon | 13282728 | 13282859 | 13282728 | ID=MS.gene30198.t1.exon9;Parent=MS.gene30198.t1 |
| chr6.3 | CDS | 13282728 | 13282859 | 13282728 | ID=cds.MS.gene30198.t1;Parent=MS.gene30198.t1 |
| chr6.3 | exon | 13282025 | 13282304 | 13282025 | ID=MS.gene30198.t1.exon10;Parent=MS.gene30198.t1 |
| chr6.3 | CDS | 13282025 | 13282304 | 13282025 | ID=cds.MS.gene30198.t1;Parent=MS.gene30198.t1 |
| chr6.3 | exon | 13281733 | 13281932 | 13281733 | ID=MS.gene30198.t1.exon11;Parent=MS.gene30198.t1 |
| chr6.3 | CDS | 13281733 | 13281932 | 13281733 | ID=cds.MS.gene30198.t1;Parent=MS.gene30198.t1 |
| chr6.3 | exon | 13281066 | 13281220 | 13281066 | ID=MS.gene30198.t1.exon12;Parent=MS.gene30198.t1 |
| chr6.3 | CDS | 13281066 | 13281220 | 13281066 | ID=cds.MS.gene30198.t1;Parent=MS.gene30198.t1 |
| chr6.3 | exon | 13280752 | 13280949 | 13280752 | ID=MS.gene30198.t1.exon13;Parent=MS.gene30198.t1 |
| chr6.3 | CDS | 13280752 | 13280949 | 13280752 | ID=cds.MS.gene30198.t1;Parent=MS.gene30198.t1 |
| chr6.3 | exon | 13280569 | 13280659 | 13280569 | ID=MS.gene30198.t1.exon14;Parent=MS.gene30198.t1 |
| chr6.3 | CDS | 13280569 | 13280659 | 13280569 | ID=cds.MS.gene30198.t1;Parent=MS.gene30198.t1 |
| chr6.3 | exon | 13280303 | 13280468 | 13280303 | ID=MS.gene30198.t1.exon15;Parent=MS.gene30198.t1 |
| chr6.3 | CDS | 13280303 | 13280468 | 13280303 | ID=cds.MS.gene30198.t1;Parent=MS.gene30198.t1 |
| chr6.3 | exon | 13279580 | 13279690 | 13279580 | ID=MS.gene30198.t1.exon16;Parent=MS.gene30198.t1 |
| chr6.3 | CDS | 13279580 | 13279690 | 13279580 | ID=cds.MS.gene30198.t1;Parent=MS.gene30198.t1 |
| chr6.3 | exon | 13279351 | 13279487 | 13279351 | ID=MS.gene30198.t1.exon17;Parent=MS.gene30198.t1 |
| chr6.3 | CDS | 13279351 | 13279487 | 13279351 | ID=cds.MS.gene30198.t1;Parent=MS.gene30198.t1 |
| Gene Sequence |
| Protein sequence |
