AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32276


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32276.t1 MTR_8g461390 95.154 227 11 0 1 227 1 227 5.23e-166 457
MS.gene32276.t1 MTR_4g105470 41.667 180 96 4 39 215 23 196 3.83e-33 119
MS.gene32276.t1 MTR_8g069755 35.096 208 121 5 13 215 5 203 1.40e-31 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32276.t1 AT1G17100 60.396 202 75 2 25 221 30 231 1.87e-90 266
MS.gene32276.t1 AT1G78450 48.214 224 103 4 4 222 1 216 4.99e-71 216
MS.gene32276.t1 AT1G78460 41.518 224 105 5 11 220 8 219 8.87e-50 162

Find 46 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTATCTCAATTTCATTATC+AGG 0.236313 8.3:-48890587 MS.gene32276:CDS
TTCTTGTTTGATTTGGAAAA+TGG 0.250380 8.3:-48889009 MS.gene32276:CDS
GACGTTATAATTCGTCTGTT+TGG 0.282437 8.3:-48890478 MS.gene32276:CDS
TAGGCTATTTGATTACATTC+AGG 0.303343 8.3:-48889409 MS.gene32276:CDS
GGATTTGCTTGGTTCACTTT+TGG 0.319811 8.3:+48889276 None:intergenic
GATATGGTTCTTGTTTGATT+TGG 0.325272 8.3:-48889016 MS.gene32276:CDS
TGAACATGAAGACCCTTTGC+AGG 0.347285 8.3:+48889249 None:intergenic
AATGTCTTGAATAGGAGAAT+TGG 0.347748 8.3:+48890451 None:intergenic
ATTCAAAAGGGGCATTGTAC+TGG 0.369508 8.3:+48889058 None:intergenic
CTTGTTGAAGCTACTAGAAC+TGG 0.386778 8.3:-48890425 MS.gene32276:CDS
ACAAATGAAGACTCACAAAA+TGG 0.387846 8.3:+48889315 None:intergenic
AGCTTCTATGACATCATAGT+TGG 0.390370 8.3:+48890520 None:intergenic
ACCCTATTATCATATTCAAA+AGG 0.401311 8.3:+48889045 None:intergenic
ACAAGAGAAATGTCTTGAAT+AGG 0.403479 8.3:+48890443 None:intergenic
CCCTATTATCATATTCAAAA+GGG 0.409363 8.3:+48889046 None:intergenic
TGCGGAAGCTGCAGCGTTGA+AGG 0.431888 8.3:-48889160 MS.gene32276:CDS
AGGCTATTTGATTACATTCA+GGG 0.450840 8.3:-48889408 MS.gene32276:CDS
TGAAGACCCTTTGCAGGTGG+TGG 0.452104 8.3:+48889255 None:intergenic
TTGCAGGTGGTGGATTTGCT+TGG 0.453762 8.3:+48889265 None:intergenic
AACTATGATGTCATAGAAGC+TGG 0.463929 8.3:-48890518 MS.gene32276:CDS
TATGCAGCAGTTAGACAGTT+TGG 0.464469 8.3:-48889210 MS.gene32276:CDS
ATAATAGGGTGAATGAGATA+TGG 0.465751 8.3:-48889032 MS.gene32276:CDS
GCGTTGAAGGACAGTATTGC+CGG 0.485645 8.3:-48889147 MS.gene32276:CDS
GATGTCATAGAAGCTGGAAA+TGG 0.490076 8.3:-48890512 MS.gene32276:CDS
AGCAAATCCACCACCTGCAA+AGG 0.503511 8.3:-48889262 MS.gene32276:CDS
AGGGTCTTCATGTTCAAAGA+TGG 0.509198 8.3:-48889242 MS.gene32276:CDS
GGTAAGACTTCAGAGAGAAC+AGG 0.536541 8.3:+48889348 None:intergenic
TTCAAAAGGGGCATTGTACT+GGG 0.538233 8.3:+48889059 None:intergenic
AACTCGAAGCATGCAATCCA+TGG 0.545237 8.3:-48890651 None:intergenic
GGACACTCTATGCGTTTGCA+TGG 0.549178 8.3:+48890542 None:intergenic
GCAAATCCACCACCTGCAAA+GGG 0.549447 8.3:-48889261 MS.gene32276:CDS
ATTGAACAAAGCCGTAGAGC+TGG 0.550259 8.3:-48889105 MS.gene32276:CDS
GCTTCTATGACATCATAGTT+GGG 0.553146 8.3:+48890521 None:intergenic
TGAGAGCTTCAATGTTGTGC+TGG 0.559259 8.3:+48890622 None:intergenic
TCACAAAATGGTCCATCACT+TGG 0.562399 8.3:+48889327 None:intergenic
TCTGAAGTCTTACCAAGTGA+TGG 0.564116 8.3:-48889339 MS.gene32276:CDS
GCAGCAGTTAGACAGTTTGG+TGG 0.565089 8.3:-48889207 MS.gene32276:CDS
CTGAAGCATGACCAGCTCTA+CGG 0.568630 8.3:+48889094 None:intergenic
GGCTATTTGATTACATTCAG+GGG 0.574090 8.3:-48889407 MS.gene32276:CDS
TGTTGTGCTGGTTACAGCCA+TGG 0.575258 8.3:+48890634 None:intergenic
TTTGTCAAAGATACAGATGT+TGG 0.582094 8.3:-48889183 MS.gene32276:CDS
AGCAGAAGACCACTTAGTAC+CGG 0.588835 8.3:+48889128 None:intergenic
ACAGTATTGCCGGTACTAAG+TGG 0.612734 8.3:-48889137 MS.gene32276:CDS
CCTATTATCATATTCAAAAG+GGG 0.627523 8.3:+48889047 None:intergenic
CAAAGATACAGATGTTGGTG+CGG 0.660769 8.3:-48889178 MS.gene32276:CDS
ACATGAAGACCCTTTGCAGG+TGG 0.699868 8.3:+48889252 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGTTGAATACATATAAAA+GGG - chr8.3:48889487-48889506 MS.gene32276:intron 15.0%
!! AATGTGAAATGATTTATATT+TGG - chr8.3:48889925-48889944 MS.gene32276:intron 15.0%
!! GAAAGTTGAATACATATAAA+AGG - chr8.3:48889486-48889505 MS.gene32276:intron 20.0%
!!! AAATTAATTGTCTCTAGAAT+TGG + chr8.3:48890002-48890021 None:intergenic 20.0%
!!! AGTTTTTTCAGTAATAGTTT+AGG - chr8.3:48889261-48889280 MS.gene32276:CDS 20.0%
!!! TCAATCTTTTTCTTTTTCTT+AGG - chr8.3:48890206-48890225 MS.gene32276:intron 20.0%
! AACTAATTCTAACTTGAAGT+TGG + chr8.3:48889747-48889766 None:intergenic 25.0%
! ACCCTATTATCATATTCAAA+AGG + chr8.3:48890592-48890611 None:intergenic 25.0%
! ATGCTAAAATCAATTGTTTC+TGG - chr8.3:48890041-48890060 MS.gene32276:intron 25.0%
! CCCTATTATCATATTCAAAA+GGG + chr8.3:48890591-48890610 None:intergenic 25.0%
! CCTATTATCATATTCAAAAG+GGG + chr8.3:48890590-48890609 None:intergenic 25.0%
! CTTATCTCAATTTCATTATC+AGG - chr8.3:48889047-48889066 MS.gene32276:CDS 25.0%
! TGAACTTAATGATCATTCAT+TGG + chr8.3:48890112-48890131 None:intergenic 25.0%
!! CCCTTTTGAATATGATAATA+GGG - chr8.3:48890588-48890607 MS.gene32276:CDS 25.0%
!!! AAGTTTTAATGAAGTGTTTG+TGG - chr8.3:48890146-48890165 MS.gene32276:intron 25.0%
!!! ACAATTGATTTTAGCATGTT+TGG + chr8.3:48890038-48890057 None:intergenic 25.0%
!!! TTCTTGTTTGATTTGGAAAA+TGG - chr8.3:48890625-48890644 MS.gene32276:CDS 25.0%
AATGTCTTGAATAGGAGAAT+TGG + chr8.3:48889186-48889205 None:intergenic 30.0%
ACAAATGAAGACTCACAAAA+TGG + chr8.3:48890322-48890341 None:intergenic 30.0%
ACAAGAGAAATGTCTTGAAT+AGG + chr8.3:48889194-48889213 None:intergenic 30.0%
AGGCTATTTGATTACATTCA+GGG - chr8.3:48890226-48890245 MS.gene32276:intron 30.0%
ATAATAGGGTGAATGAGATA+TGG - chr8.3:48890602-48890621 MS.gene32276:CDS 30.0%
GATCAAGTTTAGTAACTCTT+CGG + chr8.3:48890188-48890207 None:intergenic 30.0%
TAGGCTATTTGATTACATTC+AGG - chr8.3:48890225-48890244 MS.gene32276:intron 30.0%
TAGTGTGATTGGAATTGATT+TGG - chr8.3:48889413-48889432 MS.gene32276:intron 30.0%
TTTAGTGTAATTACAAGCGT+TGG - chr8.3:48889621-48889640 MS.gene32276:intron 30.0%
TTTCAAACTATTAGCTGTGT+CGG + chr8.3:48889587-48889606 None:intergenic 30.0%
TTTGTCAAAGATACAGATGT+TGG - chr8.3:48890451-48890470 MS.gene32276:CDS 30.0%
! CCCCTTTTGAATATGATAAT+AGG - chr8.3:48890587-48890606 MS.gene32276:CDS 30.0%
! GATATGGTTCTTGTTTGATT+TGG - chr8.3:48890618-48890637 MS.gene32276:CDS 30.0%
!! AATTAGTATCTTTTGACTCC+AGG + chr8.3:48889724-48889743 None:intergenic 30.0%
!! GAGTTTGATTTTGACATGTT+TGG + chr8.3:48889802-48889821 None:intergenic 30.0%
AACTATGATGTCATAGAAGC+TGG - chr8.3:48889116-48889135 MS.gene32276:CDS 35.0%
AAGTTTAGTAACTCTTCGGA+TGG + chr8.3:48890184-48890203 None:intergenic 35.0%
AGCTTCTATGACATCATAGT+TGG + chr8.3:48889117-48889136 None:intergenic 35.0%
AGTTTAGTAACTCTTCGGAT+GGG + chr8.3:48890183-48890202 None:intergenic 35.0%
GACGTTATAATTCGTCTGTT+TGG - chr8.3:48889156-48889175 MS.gene32276:CDS 35.0%
GCTTCTATGACATCATAGTT+GGG + chr8.3:48889116-48889135 None:intergenic 35.0%
GGCTATTTGATTACATTCAG+GGG - chr8.3:48890227-48890246 MS.gene32276:intron 35.0%
! TTAGTGTGTGTTTGGTTTCA+CGG + chr8.3:48890088-48890107 None:intergenic 35.0%
!! TCATTGGTTTAGTGTGTGTT+TGG + chr8.3:48890096-48890115 None:intergenic 35.0%
!!! CTACTAGAACTGGTTTTTTG+AGG - chr8.3:48889219-48889238 MS.gene32276:CDS 35.0%
!!! CTGTCATCTCAATTTTTTGC+TGG + chr8.3:48890264-48890283 None:intergenic 35.0%
ACACTTCCCAACGTAAAAAG+TGG + chr8.3:48889828-48889847 None:intergenic 40.0%
AGGGTCTTCATGTTCAAAGA+TGG - chr8.3:48890392-48890411 MS.gene32276:intron 40.0%
CAAAGATACAGATGTTGGTG+CGG - chr8.3:48890456-48890475 MS.gene32276:CDS 40.0%
CACTTCCCAACGTAAAAAGT+GGG + chr8.3:48889827-48889846 None:intergenic 40.0%
CTTGTTGAAGCTACTAGAAC+TGG - chr8.3:48889209-48889228 MS.gene32276:CDS 40.0%
GATGTCATAGAAGCTGGAAA+TGG - chr8.3:48889122-48889141 MS.gene32276:CDS 40.0%
GGGAGTTGAAAAATCATGTC+TGG - chr8.3:48889507-48889526 MS.gene32276:intron 40.0%
GTGTTTGCTTCTAGTGTGAT+TGG - chr8.3:48889402-48889421 MS.gene32276:CDS 40.0%
TATGCAGCAGTTAGACAGTT+TGG - chr8.3:48890424-48890443 MS.gene32276:CDS 40.0%
TATTAAGCACAGACACTCCT+CGG + chr8.3:48889699-48889718 None:intergenic 40.0%
TCACAAAATGGTCCATCACT+TGG + chr8.3:48890310-48890329 None:intergenic 40.0%
! TCTGAAGTCTTACCAAGTGA+TGG - chr8.3:48890295-48890314 MS.gene32276:intron 40.0%
!! ATTCAAAAGGGGCATTGTAC+TGG + chr8.3:48890579-48890598 None:intergenic 40.0%
!! GGATTTGCTTGGTTCACTTT+TGG + chr8.3:48890361-48890380 None:intergenic 40.0%
!! TTCAAAAGGGGCATTGTACT+GGG + chr8.3:48890578-48890597 None:intergenic 40.0%
!!! ACTCTTCCCACTTTTTACGT+TGG - chr8.3:48889818-48889837 MS.gene32276:intron 40.0%
!!! CTCTTCCCACTTTTTACGTT+GGG - chr8.3:48889819-48889838 MS.gene32276:intron 40.0%
ACAGTATTGCCGGTACTAAG+TGG - chr8.3:48890497-48890516 MS.gene32276:CDS 45.0%
AGCAGAAGACCACTTAGTAC+CGG + chr8.3:48890509-48890528 None:intergenic 45.0%
ATTGAACAAAGCCGTAGAGC+TGG - chr8.3:48890529-48890548 MS.gene32276:CDS 45.0%
GGTAAGACTTCAGAGAGAAC+AGG + chr8.3:48890289-48890308 None:intergenic 45.0%
TGAACATGAAGACCCTTTGC+AGG + chr8.3:48890388-48890407 None:intergenic 45.0%
TGAGAGCTTCAATGTTGTGC+TGG + chr8.3:48889015-48889034 None:intergenic 45.0%
! ATGTCTGGAGCTATGAAGCA+CGG - chr8.3:48889522-48889541 MS.gene32276:intron 45.0%
! TGTGTGTTTGGTTTCACGGT+GGG + chr8.3:48890084-48890103 None:intergenic 45.0%
!! GAGGAGTGTCTGTGCTTAAT+AGG - chr8.3:48889698-48889717 MS.gene32276:intron 45.0%
!! TACAAGCGTTGGTGTCATGT+CGG - chr8.3:48889632-48889651 MS.gene32276:intron 45.0%
ACATGAAGACCCTTTGCAGG+TGG + chr8.3:48890385-48890404 None:intergenic 50.0%
AGCAAATCCACCACCTGCAA+AGG - chr8.3:48890372-48890391 MS.gene32276:intron 50.0%
ATACGACACGTGTTCGACAC+TGG - chr8.3:48889661-48889680 MS.gene32276:intron 50.0%
CTGAAGCATGACCAGCTCTA+CGG + chr8.3:48890543-48890562 None:intergenic 50.0%
GCAAATCCACCACCTGCAAA+GGG - chr8.3:48890373-48890392 MS.gene32276:intron 50.0%
GCAGCAGTTAGACAGTTTGG+TGG - chr8.3:48890427-48890446 MS.gene32276:CDS 50.0%
GCGTTGAAGGACAGTATTGC+CGG - chr8.3:48890487-48890506 MS.gene32276:CDS 50.0%
GGACACTCTATGCGTTTGCA+TGG + chr8.3:48889095-48889114 None:intergenic 50.0%
GTGTCTGTGCTTAATAGGCC+TGG - chr8.3:48889703-48889722 MS.gene32276:intron 50.0%
TACGACACGTGTTCGACACT+GGG - chr8.3:48889662-48889681 MS.gene32276:intron 50.0%
! GTGTGTGTTTGGTTTCACGG+TGG + chr8.3:48890085-48890104 None:intergenic 50.0%
!! TGTTGTGCTGGTTACAGCCA+TGG + chr8.3:48889003-48889022 None:intergenic 50.0%
!! TTGCAGGTGGTGGATTTGCT+TGG + chr8.3:48890372-48890391 None:intergenic 50.0%
CTATGAAGCACGGATGCGGA+CGG - chr8.3:48889532-48889551 MS.gene32276:intron 55.0%
GCACAGACACTCCTCGGATT+AGG + chr8.3:48889693-48889712 None:intergenic 55.0%
GGAGCTATGAAGCACGGATG+CGG - chr8.3:48889528-48889547 MS.gene32276:intron 55.0%
TGAAGACCCTTTGCAGGTGG+TGG + chr8.3:48890382-48890401 None:intergenic 55.0%
ACTGGGACACGCCTAATCCG+AGG - chr8.3:48889679-48889698 MS.gene32276:intron 60.0%
GACGGACACAGGACACGACA+CGG - chr8.3:48889550-48889569 MS.gene32276:intron 60.0%
! TGCGGAAGCTGCAGCGTTGA+AGG - chr8.3:48890474-48890493 MS.gene32276:CDS 60.0%
GCACGGATGCGGACGGACAC+AGG - chr8.3:48889539-48889558 MS.gene32276:intron 70.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 48888993 48890663 48888993 ID=MS.gene32276
chr8.3 mRNA 48888993 48890663 48888993 ID=MS.gene32276.t1;Parent=MS.gene32276
chr8.3 exon 48890416 48890663 48890416 ID=MS.gene32276.t1.exon1;Parent=MS.gene32276.t1
chr8.3 CDS 48890416 48890663 48890416 ID=cds.MS.gene32276.t1;Parent=MS.gene32276.t1
chr8.3 exon 48888993 48889428 48888993 ID=MS.gene32276.t1.exon2;Parent=MS.gene32276.t1
chr8.3 CDS 48888993 48889428 48888993 ID=cds.MS.gene32276.t1;Parent=MS.gene32276.t1
Gene Sequence

>MS.gene32276

ATGCAATCCATGGCTGTAACCAGCACAACATTGAAGCTCTCACTGTTCTTGACTCTTATCTCAATTTCATTATCAGGTATTCTTTCATATGATATTATACCATGCAAACGCATAGAGTGTCCCAACTATGATGTCATAGAAGCTGGAAATGGCTATGAAATCAGACGTTATAATTCGTCTGTTTGGATTTCCAATTCTCCTATTCAAGACATTTCTCTTGTTGAAGCTACTAGAACTGGTTTTTTGAGGTAATTTATTTTTTTACTTAAGTTTTTTCAGTAATAGTTTAGGTTTTTGAATTCTATTTTCTATGAGTGCATTTGATTAGTATAGAAGAATTTCTATCAATAAGTTTATGATACAAAAATATGAAGTTTAACTTGCATGTTTGATTGAAAAAATTAAATGTGTGTTTGCTTCTAGTGTGATTGGAATTGATTTGGAATTAATTGATTTTATTTAAAATAAGCTTATTGTGAAGTGCTTTACGTTTGAAAGTTGAATACATATAAAAGGGAGTTGAAAAATCATGTCTGGAGCTATGAAGCACGGATGCGGACGGACACAGGACACGACACGGACACTGACACGCCGACACAGCTAATAGTTTGAAAAAAAAAGCGCATAATTTAGTGTAATTACAAGCGTTGGTGTCATGTCGGTGTCTGATACGACACGTGTTCGACACTGGGACACGCCTAATCCGAGGAGTGTCTGTGCTTAATAGGCCTGGAGTCAAAAGATACTAATTCCAACTTCAAGTTAGAATTAGTTGTAGCGACAAAATCAATTTTAATCGACATCATCCAAACATGTCAAAATCAAACTCTTCCCACTTTTTACGTTGGGAAGTGTGTTTGCTTTTGCATTTGACATAAATTGATTTTGAATGAATTGATTCTGTAAAATTGATTTTAATTAAAAGTAAGTTGAATGTGAAATGATTTATATTTGGATACACTCAATTAAAAATGAGTTGAGCTATAAATTAAGCTTCAAGTTAGAACCAATTCTAGAGACAATTAATTTTTATCGATATCATCCAAACATGCTAAAATCAATTGTTTCTGGAATCACTTTTGACACTTCCCACCGTGAAACCAAACACACACTAAACCAATGAATGATCATTAAGTTCATCTATACTGCAAAGAAGTTTTAATGAAGTGTTTGTGGTGCTGAATTGTCCCATCCGAAGAGTTACTAAACTTGATCAATCTTTTTCTTTTTCTTAGGCTATTTGATTACATTCAGGGGAAGAACAACTACCAGCAAAAAATTGAGATGACAGCACCTGTTCTCTCTGAAGTCTTACCAAGTGATGGACCATTTTGTGAGTCTTCATTTGTTGTCAGCTTTTATGTACCAAAAGTGAACCAAGCAAATCCACCACCTGCAAAGGGTCTTCATGTTCAAAGATGGAAAACTGTGTATGCAGCAGTTAGACAGTTTGGTGGTTTTGTCAAAGATACAGATGTTGGTGCGGAAGCTGCAGCGTTGAAGGACAGTATTGCCGGTACTAAGTGGTCTTCTGCTATTGAACAAAGCCGTAGAGCTGGTCATGCTTCAGTTTACTCTGTTGCCCAGTACAATGCCCCTTTTGAATATGATAATAGGGTGAATGAGATATGGTTCTTGTTTGATTTGGAAAATGGTTTGCATTCTATGTGA

Protein sequence

>MS.gene32276.t1

MQSMAVTSTTLKLSLFLTLISISLSGILSYDIIPCKRIECPNYDVIEAGNGYEIRRYNSSVWISNSPIQDISLVEATRTGFLRLFDYIQGKNNYQQKIEMTAPVLSEVLPSDGPFCESSFVVSFYVPKVNQANPPPAKGLHVQRWKTVYAAVRQFGGFVKDTDVGAEAAALKDSIAGTKWSSAIEQSRRAGHASVYSVAQYNAPFEYDNRVNEIWFLFDLENGLHSM