AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene019323

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene019323.t1 MTR_5g010000 95.299 468 21 1 2 468 27 494 0.0 877
MS.gene019323.t1 MTR_5g009970 85.288 469 68 1 1 468 27 495 0.0 811
MS.gene019323.t1 MTR_5g009970 85.714 413 58 1 57 468 9 421 0.0 718
MS.gene019323.t1 MTR_5g009970 85.279 394 57 1 76 468 1 394 0.0 679
MS.gene019323.t1 MTR_5g009970 85.276 326 47 1 144 468 1 326 0.0 552
MS.gene019323.t1 MTR_3g080810 54.565 460 196 6 15 464 35 491 1.66e-176 511
MS.gene019323.t1 MTR_3g117500 50.737 475 221 5 6 469 15 487 2.04e-166 485
MS.gene019323.t1 MTR_8g104820 49.571 466 220 7 11 464 26 488 4.36e-162 474
MS.gene019323.t1 MTR_5g085730 48.643 479 226 7 6 467 22 497 3.06e-160 471
MS.gene019323.t1 MTR_3g080190 48.637 477 225 7 11 470 36 509 2.31e-157 463
MS.gene019323.t1 MTR_3g105230 48.315 445 226 4 13 456 28 469 8.64e-157 460
MS.gene019323.t1 MTR_3g010330 50.437 458 215 7 15 464 25 478 5.17e-156 458
MS.gene019323.t1 MTR_3g023740 48.908 458 230 4 14 470 29 483 2.25e-155 457
MS.gene019323.t1 MTR_5g093590 49.041 469 222 7 14 466 33 500 2.14e-154 455
MS.gene019323.t1 MTR_1g088190 49.251 467 207 6 12 469 36 481 3.88e-153 451
MS.gene019323.t1 MTR_4g035955 48.387 465 231 4 5 464 4 464 4.85e-153 450
MS.gene019323.t1 MTR_8g099410 48.729 472 222 7 11 466 24 491 1.08e-151 449
MS.gene019323.t1 MTR_4g035930 47.912 455 228 4 15 464 15 465 6.15e-151 445
MS.gene019323.t1 MTR_4g074960 46.906 501 237 9 14 489 27 523 2.71e-150 449
MS.gene019323.t1 MTR_8g099410 48.729 472 222 7 11 466 24 491 4.51e-150 448
MS.gene019323.t1 MTR_4g035975 46.996 466 228 7 14 464 26 487 7.46e-147 435
MS.gene019323.t1 MTR_1g037340 48.261 460 227 6 15 466 25 481 1.81e-142 424
MS.gene019323.t1 MTR_3g113720 43.856 472 245 6 16 472 58 524 3.83e-132 399
MS.gene019323.t1 MTR_8g075990 45.299 468 236 6 16 468 64 526 1.60e-128 390
MS.gene019323.t1 MTR_5g010010 98.361 183 3 0 469 651 51 233 4.64e-128 377
MS.gene019323.t1 MTR_2g028480 39.567 508 254 13 15 485 111 602 5.60e-100 318
MS.gene019323.t1 MTR_3g090540 37.832 489 255 15 15 466 107 583 2.91e-98 313
MS.gene019323.t1 MTR_3g082440 37.295 488 264 13 15 469 116 594 1.45e-91 296
MS.gene019323.t1 MTR_1g088190 48.693 306 128 5 12 308 36 321 9.28e-86 272
MS.gene019323.t1 MTR_7g108925 47.771 157 81 1 310 466 43 198 9.28e-50 173
MS.gene019323.t1 MTR_0393s0050 48.649 148 74 2 310 456 20 166 5.45e-41 147
MS.gene019323.t1 MTR_1g037320 48.447 161 78 2 76 232 1 160 1.41e-39 147
MS.gene019323.t1 MTR_1g037320 51.639 122 57 2 344 464 169 289 4.20e-32 126
MS.gene019323.t1 MTR_4g128810 40.523 153 88 1 317 469 187 336 2.68e-31 125
MS.gene019323.t1 MTR_7g053480 70.213 47 14 0 468 514 50 96 4.38e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene019323.t1 AT4G23560 71.398 465 133 0 5 469 13 477 0.0 681
MS.gene019323.t1 AT4G09740 70.108 465 139 0 5 469 13 477 0.0 672
MS.gene019323.t1 AT1G02800 54.506 466 198 6 13 469 41 501 5.47e-176 510
MS.gene019323.t1 AT4G02290 51.732 462 211 5 12 464 49 507 3.93e-169 493
MS.gene019323.t1 AT1G23210 51.073 466 216 5 5 461 18 480 2.50e-168 490
MS.gene019323.t1 AT1G70710 51.522 460 211 5 11 461 24 480 9.35e-168 489
MS.gene019323.t1 AT4G39010 50.622 482 220 7 8 477 22 497 1.64e-166 486
MS.gene019323.t1 AT1G71380 50.740 473 221 7 8 473 17 484 5.62e-157 461
MS.gene019323.t1 AT3G43860 49.561 456 226 4 15 469 34 486 1.82e-154 454
MS.gene019323.t1 AT3G43860 49.561 456 226 4 15 469 34 486 1.82e-154 454
MS.gene019323.t1 AT1G22880 50.214 468 222 6 9 469 18 481 2.52e-153 452
MS.gene019323.t1 AT2G32990 47.210 466 230 7 14 464 38 502 2.80e-152 451
MS.gene019323.t1 AT4G11050 45.491 499 250 8 12 492 23 517 2.87e-149 443
MS.gene019323.t1 AT4G11050 45.491 499 250 8 12 492 23 517 4.73e-148 443
MS.gene019323.t1 AT4G11050 45.491 499 250 8 12 492 23 517 4.84e-148 443
MS.gene019323.t1 AT1G64390 45.110 501 251 9 12 496 22 514 1.29e-147 442
MS.gene019323.t1 AT4G39000 47.312 465 227 7 14 464 24 484 5.41e-147 436
MS.gene019323.t1 AT4G38990 46.218 476 239 6 10 469 18 492 2.44e-145 431
MS.gene019323.t1 AT2G44570 48.283 466 228 7 11 466 29 491 1.98e-144 429
MS.gene019323.t1 AT1G48930 47.826 506 241 7 14 498 29 532 3.02e-144 433
MS.gene019323.t1 AT2G44540 48.052 462 228 6 15 467 33 491 1.23e-142 424
MS.gene019323.t1 AT2G44560 48.590 461 225 6 15 466 33 490 2.09e-141 421
MS.gene019323.t1 AT2G44550 46.541 477 234 9 1 467 25 490 1.38e-138 414
MS.gene019323.t1 AT1G19940 46.288 458 230 5 15 461 48 500 2.15e-134 404
MS.gene019323.t1 AT1G19940 46.288 458 230 5 15 461 48 500 2.15e-134 404
MS.gene019323.t1 AT4G02290 48.744 398 192 5 76 464 1 395 1.37e-132 395
MS.gene019323.t1 AT1G75680 46.813 455 222 7 16 457 58 505 5.17e-131 396
MS.gene019323.t1 AT1G22880 48.628 401 195 6 76 469 1 397 9.59e-125 375
MS.gene019323.t1 AT4G39010 47.826 368 178 6 8 363 22 387 1.01e-116 354
MS.gene019323.t1 AT1G63970 83.237 173 29 0 479 651 59 231 3.59e-101 308
MS.gene019323.t1 AT2G44570 44.974 378 196 6 11 379 29 403 5.84e-101 314
MS.gene019323.t1 AT5G49720 39.448 507 258 13 15 485 108 601 1.97e-97 312
MS.gene019323.t1 AT1G19940 44.598 361 188 4 15 364 48 407 4.15e-95 299
MS.gene019323.t1 AT4G24260 38.878 517 263 15 15 493 108 609 3.01e-92 298
MS.gene019323.t1 AT1G65610 35.842 505 273 15 15 485 116 603 1.96e-91 296
MS.gene019323.t1 AT1G22880 49.515 309 146 5 9 310 18 323 2.26e-89 281
MS.gene019323.t1 AT4G38990 42.733 344 173 5 10 348 18 342 3.34e-83 266
MS.gene019323.t1 AT4G24260 37.209 387 200 12 15 364 108 488 6.43e-56 197
MS.gene019323.t1 AT1G63970 81.818 99 18 0 479 577 59 157 1.83e-55 186
MS.gene019323.t1 AT4G24260 42.657 286 131 9 15 271 108 389 3.23e-51 184
MS.gene019323.t1 AT3G42153 58.696 92 34 1 221 308 16 107 5.42e-30 114

Find 153 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGGTTATCCTTTAATTAT+TGG 0.154047 5.4:+4416163 MS.gene019323:CDS
CTCCCCTCCACTTCACTCTT+TGG 0.212758 5.4:-4412482 None:intergenic
TTAATTCCACCAATAATTAA+AGG 0.221109 5.4:-4416172 None:intergenic
ATGTCATACATGGTGGGATA+TGG 0.230083 5.4:+4414422 MS.gene019323:CDS
ATGAAAAGGATGAACTATTA+TGG 0.237262 5.4:+4413623 MS.gene019323:intron
CCAGAGTACGAGCAGTAAAA+TGG 0.238707 5.4:-4413498 None:intergenic
AACTATTATGGGCTGCTTCT+TGG 0.270350 5.4:+4413635 MS.gene019323:CDS
TCAGCTGCAGCCTCAGTTCC+AGG 0.287596 5.4:-4413109 None:intergenic
TTGTATTGGTCATTAGAATT+TGG 0.289530 5.4:-4414596 None:intergenic
CCCACATGAATATTAGGATT+TGG 0.290939 5.4:-4414557 None:intergenic
CTGCTCGTACTCTGGTTATC+AGG 0.293044 5.4:+4413506 MS.gene019323:CDS
TCAATCTTGTAAGTTGTTCT+TGG 0.295072 5.4:-4413076 None:intergenic
CGCTTCTGTGGTAAATATAA+AGG 0.303097 5.4:+4419279 MS.gene019323:CDS
CCTCTGTGATGTAATTGTTT+TGG 0.305598 5.4:-4414455 None:intergenic
CTTGGTGTATCCATGTCTTC+TGG 0.334811 5.4:-4413058 None:intergenic
ACATAGGTGGACTACATATT+AGG 0.343969 5.4:+4414383 MS.gene019323:intron
ATCTGTAGAGAGCTACTTCC+TGG 0.344038 5.4:-4413985 None:intergenic
ATAGCACCCACATGAATATT+AGG 0.348295 5.4:-4414563 None:intergenic
CATGAGGCAGGTTATGATAT+TGG 0.350566 5.4:+4419163 MS.gene019323:CDS
GTGTCATTGGCAGGTGGATT+TGG 0.356090 5.4:+4412670 MS.gene019323:intron
CATTGGCAGGTGGATTTGGT+TGG 0.371328 5.4:+4412674 MS.gene019323:intron
TCTTCGCAGTGCTATTCGTT+GGG 0.375871 5.4:+4412820 MS.gene019323:CDS
GCTTTAGGTCTTCCTGATAT+AGG 0.382227 5.4:+4417198 MS.gene019323:CDS
TTGAAGGAGTAGCGGAGATT+GGG 0.383157 5.4:-4416090 None:intergenic
AGCTGGGATAACAAATTTGT+TGG 0.384877 5.4:+4413739 MS.gene019323:CDS
TCCTTGTGTGGGCTTAGTTT+CGG 0.392429 5.4:-4419217 None:intergenic
TTTGAAGGAGTAGCGGAGAT+TGG 0.395299 5.4:-4416091 None:intergenic
CAGATCTTGCATCATTGTAT+TGG 0.398459 5.4:-4414610 None:intergenic
GGTTCTGTTGCAGCTTTGCT+TGG 0.409254 5.4:+4414680 MS.gene019323:CDS
CACCATGTATGACATCTTCA+TGG 0.417078 5.4:-4414414 None:intergenic
TCATTGCACCCCACTTTGGC+AGG 0.417272 5.4:-4414506 None:intergenic
TTCACCATCAGTCTTAATCT+TGG 0.417439 5.4:-4413941 None:intergenic
ATCTTCGCAGTGCTATTCGT+TGG 0.429802 5.4:+4412819 MS.gene019323:CDS
CACTTCACTCTTTGGTCCTT+AGG 0.430996 5.4:-4412474 None:intergenic
ATCGATTTCAACTGAGGCTG+TGG 0.440478 5.4:-4416062 None:intergenic
TGGCAGGTGGATTTGGTTGG+AGG 0.444755 5.4:+4412677 MS.gene019323:intron
GTGGAAGGGTTGTGATTCTT+CGG 0.447373 5.4:+4417248 MS.gene019323:CDS
TGAAAAGGATGAACTATTAT+GGG 0.456313 5.4:+4413624 MS.gene019323:intron
TGGAACTGAGGCTGCAGCTG+AGG 0.459683 5.4:+4413111 MS.gene019323:CDS
GGTTATCCTTTAATTATTGG+TGG 0.460157 5.4:+4416166 MS.gene019323:CDS
AGAATATACTCACAGATTCA+TGG 0.463953 5.4:-4417278 None:intergenic
ATTCATGTGGGTGCTATTGT+TGG 0.466637 5.4:+4414569 MS.gene019323:CDS
AAGCAGTGTCTGAATTCAGC+TGG 0.468104 5.4:+4413722 MS.gene019323:CDS
CATGAAAAGGTTGATAGCCT+TGG 0.468863 5.4:+4419310 MS.gene019323:CDS
GAAACGCAGATCATAATTGT+TGG 0.470658 5.4:+4413029 MS.gene019323:CDS
TAGATGAAGAGAAATAGTTC+TGG 0.473162 5.4:-4414532 None:intergenic
CCCACACAAGGATGCTATCA+AGG 0.475088 5.4:+4419228 MS.gene019323:CDS
GCGGGTGATAATGTGAAGTT+TGG 0.476392 5.4:+4412710 MS.gene019323:CDS
GCCATCATTGCACCCCACTT+TGG 0.479110 5.4:-4414510 None:intergenic
TTGAGATATGATTGAAGGGA+TGG 0.481296 5.4:-4414483 None:intergenic
TTACGGTGAGCTTGTTGAGT+TGG 0.481846 5.4:+4412747 MS.gene019323:CDS
GCCAAAGTGGGGTGCAATGA+TGG 0.482616 5.4:+4414509 MS.gene019323:CDS
GCAGGTTGAGATATGATTGA+AGG 0.483094 5.4:-4414488 None:intergenic
AAACCATGGCCAACCCGAAA+AGG 0.483370 5.4:-4416121 None:intergenic
TGCAAAGGCTCTAATTTCAG+AGG 0.483766 5.4:-4414276 None:intergenic
GCCTTGATAGCATCCTTGTG+TGG 0.485497 5.4:-4419229 None:intergenic
TCCAAGCAGTGCAGATAAGT+TGG 0.486196 5.4:-4419251 None:intergenic
TGTCATACATGGTGGGATAT+GGG 0.486645 5.4:+4414423 MS.gene019323:CDS
TAGCACCATGGTTGAGGTTG+TGG 0.489282 5.4:-4414183 None:intergenic
CAGAGTACGAGCAGTAAAAT+GGG 0.494790 5.4:-4413497 None:intergenic
TTCCACTTAGGATCAGAATC+AGG 0.496430 5.4:-4417231 None:intergenic
ATATGAAAGTGAGATTTCTT+CGG 0.496777 5.4:+4412781 MS.gene019323:CDS
GACCTTCATCGATTGGAACC+TGG 0.499572 5.4:+4416145 MS.gene019323:CDS
AACAAAAGCAGCATTCATAT+AGG 0.500246 5.4:-4414657 None:intergenic
AAGATTGATTCGAATTCCCC+TGG 0.503739 5.4:+4413091 MS.gene019323:CDS
ACACTGCTTGACTCCAACCT+TGG 0.505167 5.4:-4413708 None:intergenic
CCTTGATAGCATCCTTGTGT+GGG 0.507271 5.4:-4419228 None:intergenic
CGAAAAGGAAGAACCTTTGA+AGG 0.510185 5.4:-4416106 None:intergenic
GGAATTCTATGGCGGGAAGA+AGG 0.510525 5.4:+4413912 MS.gene019323:intron
ATTAATATACCTCATGATAG+AGG 0.512773 5.4:+4416190 MS.gene019323:CDS
GCCAACTTATCTGCACTGCT+TGG 0.513880 5.4:+4419250 MS.gene019323:CDS
TTTATATGTGCATTGATGCC+AGG 0.514115 5.4:+4413967 MS.gene019323:CDS
GTGATAATGTGAAGTTTGGT+TGG 0.518804 5.4:+4412714 MS.gene019323:CDS
AATGAATAGCACCATGGTTG+AGG 0.521095 5.4:-4414189 None:intergenic
CAGCTGCAGCCTCAGTTCCA+GGG 0.523289 5.4:-4413108 None:intergenic
GGCTGCTTCTTGGCTATACA+AGG 0.523550 5.4:+4413645 MS.gene019323:CDS
AAACGCAGATCATAATTGTT+GGG 0.523983 5.4:+4413030 MS.gene019323:CDS
CATGTGGGTGCTATTGTTGG+AGG 0.529821 5.4:+4414572 MS.gene019323:CDS
CAAATATAGAGGTTCTTACT+CGG 0.533103 5.4:+4413467 MS.gene019323:CDS
GTTGGAGGATACTACGACGC+GGG 0.534176 5.4:+4412692 MS.gene019323:CDS
TTCCTGATTCTGATCCTAAG+TGG 0.534340 5.4:+4417229 MS.gene019323:CDS
TAGAGCCTTTGCAAAATCAC+AGG 0.537879 5.4:+4414286 MS.gene019323:CDS
CATATCTCAACCTGCCAAAG+TGG 0.541021 5.4:+4414496 MS.gene019323:CDS
CTTGGCTATACAAGGCTAGT+GGG 0.541408 5.4:+4413653 MS.gene019323:CDS
GTCAGACTTATGCATGAGGC+AGG 0.542152 5.4:+4419151 MS.gene019323:CDS
ATATCTCAACCTGCCAAAGT+GGG 0.542805 5.4:+4414497 MS.gene019323:CDS
TCGATTTCAACTGAGGCTGT+GGG 0.542972 5.4:-4416061 None:intergenic
GGAGACTCGGCTCTCTCTGA+TGG 0.543154 5.4:+4412501 MS.gene019323:CDS
TCCAAATCCTAATATTCATG+TGG 0.544345 5.4:+4414556 MS.gene019323:CDS
CCAATAATTAAAGGATAACC+AGG 0.547141 5.4:-4416163 None:intergenic
TTCGAATTCCCCTGGAACTG+AGG 0.548903 5.4:+4413099 MS.gene019323:CDS
CTTCGCAGTGCTATTCGTTG+GGG 0.551133 5.4:+4412821 MS.gene019323:CDS
GAATCACAACCCTTCCACTT+AGG 0.553850 5.4:-4417243 None:intergenic
TGATTCTGATCCTAAGTGGA+AGG 0.553947 5.4:+4417233 MS.gene019323:CDS
AATCTCCGCTACTCCTTCAA+AGG 0.556988 5.4:+4416093 MS.gene019323:CDS
CACTGCGAAGATAATCAAGC+TGG 0.565610 5.4:-4412808 None:intergenic
AACCAGGTTCCAATCGATGA+AGG 0.565616 5.4:-4416147 None:intergenic
AAATATAGAGGTTCTTACTC+GGG 0.568349 5.4:+4413468 MS.gene019323:CDS
GCAAAGGCTCTAATTTCAGA+GGG 0.569361 5.4:-4414275 None:intergenic
TGTGATTGCTAGGTGGGAGA+TGG 0.571605 5.4:+4413007 MS.gene019323:intron
TTCTCAAGGAATTCTATGGC+GGG 0.576719 5.4:+4413905 MS.gene019323:intron
GGATTTATCGATTTCAACTG+AGG 0.577148 5.4:-4416068 None:intergenic
AGTGAAGTGGAGGGGAGACT+CGG 0.577463 5.4:+4412488 MS.gene019323:CDS
ACATCACAAGTAACCAAGGT+TGG 0.578483 5.4:+4413695 MS.gene019323:CDS
TTTCTCAAGGAATTCTATGG+CGG 0.583307 5.4:+4413904 MS.gene019323:intron
GACCAAAGAGTGAAGTGGAG+GGG 0.583433 5.4:+4412480 MS.gene019323:CDS
TCTTGGCTATACAAGGCTAG+TGG 0.585201 5.4:+4413652 MS.gene019323:CDS
AGCTGCAGCCTCAGTTCCAG+GGG 0.585982 5.4:-4413107 None:intergenic
GGACCAAAGAGTGAAGTGGA+GGG 0.589111 5.4:+4412479 MS.gene019323:CDS
TGAAGGAGTAGCGGAGATTG+GGG 0.589337 5.4:-4416089 None:intergenic
TTGGGAGCGTCCAGAAGACA+TGG 0.591746 5.4:+4413048 MS.gene019323:CDS
GGTTGCGAGGCTCATTCTGA+TGG 0.591970 5.4:+4416211 MS.gene019323:CDS
ACCAACCACGCTCTTTACAC+AGG 0.592498 5.4:+4412874 MS.gene019323:CDS
CTACAGATTAGAACAACTCC+AGG 0.598543 5.4:+4414000 MS.gene019323:CDS
GCTTGGAGTTGACGCTTCTG+TGG 0.600936 5.4:+4419267 MS.gene019323:CDS
CCAAATCCTAATATTCATGT+GGG 0.601860 5.4:+4414557 MS.gene019323:CDS
CAGGTTGAGATATGATTGAA+GGG 0.604313 5.4:-4414487 None:intergenic
ACCATGTATGACATCTTCAT+GGG 0.604739 5.4:-4414413 None:intergenic
ACGCTCAGGGAAGTTGCCTA+AGG 0.607352 5.4:+4412458 MS.gene019323:CDS
TCCCATGAAGATGTCATACA+TGG 0.607679 5.4:+4414412 MS.gene019323:CDS
GGATGTTCTGCTTCACTGCG+TGG 0.611052 5.4:+4417158 MS.gene019323:CDS
GCAGCATTCATATAGGTGGT+AGG 0.611057 5.4:-4414650 None:intergenic
AAGAACCTTTGAAGGAGTAG+CGG 0.614741 5.4:-4416098 None:intergenic
AGCTCAAACATTACTAACAC+AGG 0.615406 5.4:+4413762 MS.gene019323:CDS
AAAAGCAGCATTCATATAGG+TGG 0.619409 5.4:-4414654 None:intergenic
ACCTCATGATAGAGGTTGCG+AGG 0.621758 5.4:+4416198 MS.gene019323:CDS
ACCTGTGTAAAGAGCGTGGT+TGG 0.623720 5.4:-4412875 None:intergenic
AATTGAAGCAGCAGAAAGAG+CGG 0.625859 5.4:-4413140 None:intergenic
GCCGAAACTAAGCCCACACA+AGG 0.626457 5.4:+4419216 MS.gene019323:CDS
ATGCAACATTGATACTTCAG+AGG 0.627600 5.4:+4419194 MS.gene019323:CDS
TTAGCCAAGATTAAGACTGA+TGG 0.628467 5.4:+4413937 MS.gene019323:CDS
AGCAGTGTCTGAATTCAGCT+GGG 0.628902 5.4:+4413723 MS.gene019323:CDS
ATGAAGATGTCATACATGGT+GGG 0.629064 5.4:+4414416 MS.gene019323:CDS
AGGACCAAAGAGTGAAGTGG+AGG 0.629253 5.4:+4412478 MS.gene019323:CDS
GATTCTGATCCTAAGTGGAA+GGG 0.630398 5.4:+4417234 MS.gene019323:CDS
ATCGATGAAGGTCAAAACCA+TGG 0.631860 5.4:-4416135 None:intergenic
AAAGGCTCTAATTTCAGAGG+GGG 0.637646 5.4:-4414273 None:intergenic
TGCATAGTCATAATTAGCTG+AGG 0.638379 5.4:-4412397 None:intergenic
TTGGCTATACAAGGCTAGTG+GGG 0.646701 5.4:+4413654 MS.gene019323:CDS
GCCTCGCAACCTCTATCATG+AGG 0.647110 5.4:-4416199 None:intergenic
CATGAAGATGTCATACATGG+TGG 0.650028 5.4:+4414415 MS.gene019323:CDS
GGAGAAAATGAATAGCACCA+TGG 0.650294 5.4:-4414195 None:intergenic
ATATACCACAACCTCAACCA+TGG 0.660137 5.4:+4414178 MS.gene019323:CDS
CTAAGGACCAAAGAGTGAAG+TGG 0.660145 5.4:+4412475 MS.gene019323:CDS
TATCTCAACCTGCCAAAGTG+GGG 0.664840 5.4:+4414498 MS.gene019323:CDS
AAGCTCACCGTAAAAGACAT+CGG 0.669269 5.4:-4412737 None:intergenic
TCACACCTGTGTAAAGAGCG+TGG 0.687393 5.4:-4412879 None:intergenic
ACGTACATCACAAGTAACCA+AGG 0.694288 5.4:+4413691 MS.gene019323:CDS
ACAGGTCAGACTTATGCATG+AGG 0.696663 5.4:+4419147 MS.gene019323:intron
CATATGGATGGAATTCAGTG+TGG 0.696848 5.4:+4414236 MS.gene019323:CDS
CAAAGGCTCTAATTTCAGAG+GGG 0.704501 5.4:-4414274 None:intergenic
CCAAAACAATTACATCACAG+AGG 0.744075 5.4:+4414455 MS.gene019323:CDS
GGTTGGAGGATACTACGACG+CGG 0.797840 5.4:+4412691 MS.gene019323:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr5.4 gene 4412369 4419384 4412369 ID=MS.gene019323
chr5.4 mRNA 4412369 4419384 4412369 ID=MS.gene019323.t1;Parent=MS.gene019323
chr5.4 exon 4412369 4412536 4412369 ID=MS.gene019323.t1.exon1;Parent=MS.gene019323.t1
chr5.4 CDS 4412369 4412536 4412369 ID=cds.MS.gene019323.t1;Parent=MS.gene019323.t1
chr5.4 exon 4412683 4412895 4412683 ID=MS.gene019323.t1.exon2;Parent=MS.gene019323.t1
chr5.4 CDS 4412683 4412895 4412683 ID=cds.MS.gene019323.t1;Parent=MS.gene019323.t1
chr5.4 exon 4413019 4413219 4413019 ID=MS.gene019323.t1.exon3;Parent=MS.gene019323.t1
chr5.4 CDS 4413019 4413219 4413019 ID=cds.MS.gene019323.t1;Parent=MS.gene019323.t1
chr5.4 exon 4413450 4413527 4413450 ID=MS.gene019323.t1.exon4;Parent=MS.gene019323.t1
chr5.4 CDS 4413450 4413527 4413450 ID=cds.MS.gene019323.t1;Parent=MS.gene019323.t1
chr5.4 exon 4413631 4413783 4413631 ID=MS.gene019323.t1.exon5;Parent=MS.gene019323.t1
chr5.4 CDS 4413631 4413783 4413631 ID=cds.MS.gene019323.t1;Parent=MS.gene019323.t1
chr5.4 exon 4413913 4414021 4413913 ID=MS.gene019323.t1.exon6;Parent=MS.gene019323.t1
chr5.4 CDS 4413913 4414021 4413913 ID=cds.MS.gene019323.t1;Parent=MS.gene019323.t1
chr5.4 exon 4414141 4414307 4414141 ID=MS.gene019323.t1.exon7;Parent=MS.gene019323.t1
chr5.4 CDS 4414141 4414307 4414141 ID=cds.MS.gene019323.t1;Parent=MS.gene019323.t1
chr5.4 exon 4414389 4414701 4414389 ID=MS.gene019323.t1.exon8;Parent=MS.gene019323.t1
chr5.4 CDS 4414389 4414701 4414389 ID=cds.MS.gene019323.t1;Parent=MS.gene019323.t1
chr5.4 exon 4416044 4416232 4416044 ID=MS.gene019323.t1.exon9;Parent=MS.gene019323.t1
chr5.4 CDS 4416044 4416232 4416044 ID=cds.MS.gene019323.t1;Parent=MS.gene019323.t1
chr5.4 exon 4417157 4417287 4417157 ID=MS.gene019323.t1.exon10;Parent=MS.gene019323.t1
chr5.4 CDS 4417157 4417287 4417157 ID=cds.MS.gene019323.t1;Parent=MS.gene019323.t1
chr5.4 exon 4419151 4419384 4419151 ID=MS.gene019323.t1.exon11;Parent=MS.gene019323.t1
chr5.4 CDS 4419151 4419384 4419151 ID=cds.MS.gene019323.t1;Parent=MS.gene019323.t1
Gene Sequence

>MS.gene019323

ATGGATGATGAATTCATTAATACAAATTCCTCAGCTAATTATGACTATGCAGATGCTCTTGACAAAGCCATCATGTTTTTTGAAGGACAACGCTCAGGGAAGTTGCCTAAGGACCAAAGAGTGAAGTGGAGGGGAGACTCGGCTCTCTCTGATGGCAAGACTCAAAATGTATACAAATATACACGACATGACCTAAAATTTAACCACAATTTTGCACTAGGCTCAATATAACTTTATACAATGCTCTATATATGTGTGTTTTTTGATATGAATTCTTTATGAACAATCATGTGTATTGTATGTGTCATTGGCAGGTGGATTTGGTTGGAGGATACTACGACGCGGGTGATAATGTGAAGTTTGGTTGGCCGATGTCTTTTACGGTGAGCTTGTTGAGTTGGACTGCTGTTGAATATGAAAGTGAGATTTCTTCGGTGAACCAGCTTGATTATCTTCGCAGTGCTATTCGTTGGGGTGCAGATTTCATACTTAAAGCTCACACTTCACCAACCACGCTCTTTACACAGGTGTGATTCTATATATCCTATGATAATGTATACATTTTAGATAATATAATGTACACATAATGTGTGTGTGATTTGTGTTGCATGTTGATTAGTTCTATAATCAAATATCGTTGTGATTGCTAGGTGGGAGATGGAAACGCAGATCATAATTGTTGGGAGCGTCCAGAAGACATGGATACACCAAGAACAACTTACAAGATTGATTCGAATTCCCCTGGAACTGAGGCTGCAGCTGAGGCTGCTGCCGCTCTTTCTGCTGCTTCAATTGTCTTTAAGAAAACAGATATCAATTATTCATCCAAGCTATTAAGCCAGTCAAAATCTGTAAGCAATTCAAGGAACATGTTTTATTCTCATTCATGAATCATGATGATCAATTGGCTCTATAATCCATTGATCAAGAGCTAATATTTCCATTTTGATTTTATATAATTAAACTTAAGGCCATTAAAAAAAAAAAATATAATTAAACTTAAGAAACAAGTGAAATTTGCATTAGAGTGTTTATGTATTTAATTTAACAAAATTTACTAATAATTAAATATGTTTTGCAGTTGTTTGATTTTGCTGACAAATATAGAGGTTCTTACTCGGGTTCTTGCCCATTTTACTGCTCGTACTCTGGTTATCAGGTAAGAAATTACCTCTTGTAAAAAATAAAACATTTAAAGACTAATAATTATTTTTTCATAGATCATTCATTTATGGTTGTTATTTTAAATTGTGTATGAAAAGGATGAACTATTATGGGCTGCTTCTTGGCTATACAAGGCTAGTGGGGAAAGCAAGTACTTGACGTACATCACAAGTAACCAAGGTTGGAGTCAAGCAGTGTCTGAATTCAGCTGGGATAACAAATTTGTTGGAGCTCAAACATTACTAACACAGGTATTTACATTTATAGCTAATTACCATTAATTACCATATAGCCAAACTGTATTCTTTTAGTAAACATTAGTTCAAAGGAAAAAACTTAACTCATTAATTCTAATTGTTTTTCTTTCTTCTTTTCTCAAGGAATTCTATGGCGGGAAGAAGGAGTTAGCCAAGATTAAGACTGATGGTGAATCATTTATATGTGCATTGATGCCAGGAAGTAGCTCTCTACAGATTAGAACAACTCCAGGTATATATGTAATGATTGCAAAGTAATATTACATATAACACAAATAGTCAATCTTATTTCAATCAACGTACATATGATGGAATGAGCTCAGTTAGTGTATTATTTTGTTGTTAACTCAGGTGGGCTTCTGTATATTAGAGATAGTAGTAATTTGCAATATACCACAACCTCAACCATGGTGCTATTCATTTTCTCCAAAATCTTAAATAAAAATCATATGGATGGAATTCAGTGTGGCTCTGCCCATTTCTCCCCCTCTGAAATTAGAGCCTTTGCAAAATCACAGGTATATATACTAATCTGGTTTTGCTTCTAAAATATCAAACTCATTGGTGCATAACTAAAACTTGAATGTGACATTACATAGGTGGACTACATATTAGGAAACAATCCCATGAAGATGTCATACATGGTGGGATATGGGAGTAAATATCCAAAACAATTACATCACAGAGGTTCTTCCATCCCTTCAATCATATCTCAACCTGCCAAAGTGGGGTGCAATGATGGCCAGAACTATTTCTCTTCATCTAATCCAAATCCTAATATTCATGTGGGTGCTATTGTTGGAGGACCAAATTCTAATGACCAATACAATGATGCAAGATCTGATTACTCTCATGCAGAACCTACCACCTATATGAATGCTGCTTTTGTTGGTTCTGTTGCAGCTTTGCTTGGTGAAACTAACACCGAGACTCTTCAATTTTCTTGAATTGGTCATGCCAAGGCTAAGGAAACTGATTAGTACTGCATAGCTGATTACTAATAATTTATCTCCTCCATATCCATGTTAAGAAAGTTAGTCTAAAGTTATTACAAGTGAACTAGTTAAATAATTTGTAAGGAAGGTTAAGTTCATACTTCAAAGGACTTTACTATTGTCGTTATTATAAGTAAAAATCTCACTTCTATTTTGAGTTGTTATTACATGTTCTTATAAAATGAAATCACATGAGTCGTGTTCTTTTATCAATGAGCAGCTCATATGTTTACTTGTGGAAAAGGGAATCTGGATACTGATTATTTCAAAGATTAATTAATTTTGAAATTTATAAACTTAAATAAAATAAGTCTTTAATGAAACTTTTGAAATAAATAAATAAAAAAACATTTAAGCCACTAGAATTGTTAGAGTGATGCGAGATGTGTGTTGCATGTATGAGATCTTAGGATAAAATCTCATTGCCTACATTGTACGTAAAAATTCAAACATTTAAAGACCTCAAGTTGAACTTTTGACAATGTCGTTCATTAATCATTTCTAATCATCCGGGCTATGTAAGATGAAGTTTGCTATAATCCATTTGAAAAATATTTGATGAACACCCAAACACCCCAACAACATAATGAGAGAATCTCTCAAAAAACAAAAAATATAATGAGAGAGAAAGAAATAGAGAAAGTGATGTGATAGATGTGATATATTAGCGATGAGATAAAAAAAAGAGATAAAGAATAAATAAATAAATAAATAAGATATTGAAACATTTGTCAAATTACTTGTTAAAACATGTTCTTAAAAAAAAAAATTAAGTTGGTTAACAAATCATCCTAGACTTTAAAAAAAGTTAGACTCAAATCTAAATTTTTTGGGACTAAGTCAAACTTAAATCTTACAAAACCTAATAATTCAACCTAATTCAAACTTAAACTTTTTGGGATGATTTAAATATATAATATTTTTTGGTACATGATGATTTAAATTTTAATCTTTGACTAAAATACAAATTCAGGGTACACACATAAAATTAACAGAATATACCAATTCTGTGCCGCTCAAATAAAACCATATGATAGTGTCTGAAAACTGAAATTCACACCATTTTTTCACAACAGTAATCTCCTTAACTTCAACAACCAACCAACCAACCATGTCTTCATCTTCTCTAACAGCATCTTCTTACTCCTTCCCATTCACAGCATCCCTCAAATCTTCTCACCCAATTCCATTCTCTTGTTCTTTTCCTCTCAAACACCATACCCTTCATCTCAAATCATCACCATCACTGTTTGTTTCAGCTTCAGGAGCTGCAACACCCACAGCCTCAGTTGAAATCGATAAATCCCCAATCTCCGCTACTCCTTCAAAGGTTCTTCCTTTTCGGGTTGGCCATGGTTTTGACCTTCATCGATTGGAACCTGGTTATCCTTTAATTATTGGTGGAATTAATATACCTCATGATAGAGGTTGCGAGGCTCATTCTGATGGTATTCATTCTATCTAACCCTTATAGCATTTTCATTTTTATAATCTTTTTTACATTTCTCCGAGTTAATAGTCATTTTGATTCTTAAATGTGTGAGACACTGTCACAATTGTTCTTCAATGTATAAAGATTTCAACAACATACATAAATTTGTCTTTTTTGTTAGTTAGTTAGTTAGTTAGTGAATATTTTCTGGTCGTCAATTTAGTTCATAAAATTATTAACGAAAGATACACTCTATGCTCTTTTTGTAATTTCGAAATATTTAGGGACTATGACTCTGTTTGGTAGAAAGTAGTGGATAGTGGATAAGTTAGTTGATAGTGGATGGACTTATAGCAGATAAGCTAGCTGATAGTGGATAAACTAGTGAATTAAATTTGTAGTGTTTTGAAAATTATACGGTATAAGCTATCCATTGTAAGTTTGTGATTAGGGGCCATTTGTTTGGATTGGCTTATTTACTATGAAACACGGACACTCCACAAATTAGGTGTGTTCCGGTGTCGGACACGCTTCGCTTCCTACACTGGCACATATGATTACACTAAATTATGTCATTTTCTCAAAATTATTATCGACATCGGTGTCATGTCCCGTGTCCGTGTCTATGTTTGTGCTTCATAGCTTATTTTTTAGCTTATGAAAAATAGTTTATGCAAATAAATAAGTTGTTATGTTAATTCATAAGTTCTCACTAACGAAAATTGTATTTTCATAAGCCGTTTTCTCATAAACTATCTTGACGAGCTTATGAATAATACATAAAAGCTTATTTATTTTCATAAGCTGTTCTGGATAAGCTAAAAAATAAGCCAATCCAAATGGGCCCAAGATCTTGCCAACAACTCTATACCAAATTGAGTATTTACTCAATTTTTTCATTTTTTTTCTGAGTTTGAACATGATGATGAAATATGAACAGGGGATGTTCTGCTTCACTGCGTGGTTGATGCAATTTTAGGGGCTTTAGGTCTTCCTGATATAGGACAGATTTTTCCTGATTCTGATCCTAAGTGGAAGGGTTGTGATTCTTCGGTGTTTGTCCATGAATCTGTGAGTATATTCTACATAAATATCTTCCTTCTACTTTATTTTCTCAATTGAAATACAGTATGATTTAGGCCTTAGTGTAATTGTGTTTTCAGTTTTTCTAATGTTGTTTGAATTTAGGGAATATATTAATAGGTTACCAAATGTTCACCGTAATGATTTGTTTGTTCTGTCACAAATTGTAATTCCATTTCAATGAATTATTCATGGACACATTGCATATTGCTTTCAAGTTATTATTATGCAGTGTTAGAACTTAAATGGTTGCTAAAGTATAAGCATGATAGGAAGGTATGCCTTGCAATCAGGGACAGTGGAATGGAAAGACATTGTCATTGTTCGGATTGTTAGAATAAGATGGAATGAAATGAGATATCAAATGCATTCCATCTGCTACATCTATTTTTCATTCTTCTAATTTAGGGTGCATGGATGAAATGACATGACTTTATTATTGAACGAGTTTGGTACGTACGAGTTTAAGAAATCATGAAATTATTACTGAGTTAAAAGGGTAAGGACTTAGAAGAGATTAGCGCTTTGGTTAACAGTGGAAATAATGACATGAGTTAACAGGCTACTGATTAGCTTGATGAGCAGAAAAGCAGACTGTTCATGTACCAATCATATACCTTGTATGTATATAAGGGAATCAGCTGTGTTCAGTTTTTTTAAAGGGGACGGGAGAAGAGAGGAAGGAGAGCAAAATCTCGCATAATACTATAACATTATAAATAGACTAAATTAACCTTAAATACCACACGAAATGTTAAAATGGTGTAAAATCTAAATTTGTATGTTCTTTTCATTAAAATTACTCATATCTCCGTATTATACCAAACAAAAAAGAGAGATGTTTTTATTCCCCTCTGTTAGAACCGAATAGACCTGTAGAGGCGGGTATGCATACCAGCTCGCACCAGTCAGCACAGGCCCATATTTAAACTGATTGGTTTGCATAATCCCTTATTTAAACAGGCATATATTTAAGAAGTTCGCATACATATGTGAGTTCAAAAGGCCGGTCTGCCTACTAATGTGCATGTTTTACTTTTCCTTGTAAAAAAATTTTTGGGTAATTTTCACTTGAAAAGTAACTTAAAAATTGTTTTTCTTTTTTATGTAGATATATGGGTTGATTTGCAAAGTTGTTCATCAAACCCATTTAATCCGGTGTCACAAACTAACATACATAGGATCACAAGGTCAGCTATTTAACTGGGCACAAATTTACATCTCCCAAACAACCGTTAGCCCTTGTACTCACCTCAACATACACACATGATACACCTCAAATATGTCATTTCCGAAGAAAAAGCTTTTATTCACTCTACAGACAGTTAGCATGTGAAGTGTGACTTCCTATTTTAAACAATTCAGATACAATTCACATCTGAAAAATGAGTAATTATTCTAACAAGCTCTATCAATTTAAGCTGATACAGACCAAAAAGTAGAGAATGAGTTCAAGGATATTACAGTAAGAAAAAATTACCTAACAGTATTCAGAAGTGAGATTGTGCTCTATCAGTCAAGTGTGTAACAGGGTAATTGATAATGTCTCTTAACCAAACAAAACTTTGCTATTTTTTTTTTTTTTAGTTAAATTTTTTGCTAGTTATTGAATAAGTAAACTGTGACCTAACAGTGACTCCTCTTTTATTGTATTAATTTTGGACCCTGCATTTACCATGTGTTTAGCAGTTATTAAACAACATTTTCTGTGATTTTCTTTTGACGTATGATGGTTGTACAAATTATATTTGGTTACTGAAAGTGTTTTCTTAGGATTATGATTAAAGATAACACTGTATCTATACTTGATATTTTTAGGGTATTTTATAATATGTTATGATTTTATTACAGGTCAGACTTATGCATGAGGCAGGTTATGATATTGGAAATTTAGATGCAACATTGATACTTCAGAGGCCGAAACTAAGCCCACACAAGGATGCTATCAAGGCCAACTTATCTGCACTGCTTGGAGTTGACGCTTCTGTGGTAAATATAAAGGCAAAAACTCATGAAAAGGTTGATAGCCTTGGAGAAAATAGAAGTATAGCAGCTCACACAGTTGTTCTTCTAATGAAGAAATGA
Protein sequence

>MS.gene019323.t1

MDDEFINTNSSANYDYADALDKAIMFFEGQRSGKLPKDQRVKWRGDSALSDGKTQNVDLVGGYYDAGDNVKFGWPMSFTVSLLSWTAVEYESEISSVNQLDYLRSAIRWGADFILKAHTSPTTLFTQVGDGNADHNCWERPEDMDTPRTTYKIDSNSPGTEAAAEAAAALSAASIVFKKTDINYSSKLLSQSKSLFDFADKYRGSYSGSCPFYCSYSGYQDELLWAASWLYKASGESKYLTYITSNQGWSQAVSEFSWDNKFVGAQTLLTQEFYGGKKELAKIKTDGESFICALMPGSSSLQIRTTPGGLLYIRDSSNLQYTTTSTMVLFIFSKILNKNHMDGIQCGSAHFSPSEIRAFAKSQVDYILGNNPMKMSYMVGYGSKYPKQLHHRGSSIPSIISQPAKVGCNDGQNYFSSSNPNPNIHVGAIVGGPNSNDQYNDARSDYSHAEPTTYMNAAFVGSVAALLASGAATPTASVEIDKSPISATPSKVLPFRVGHGFDLHRLEPGYPLIIGGINIPHDRGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGCDSSVFVHESVRLMHEAGYDIGNLDATLILQRPKLSPHKDAIKANLSALLGVDASVVNIKAKTHEKVDSLGENRSIAAHTVVLLMKK